MD10G1262400.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016008
Physical Location & Seq
Forward (+)
35702543 .. 35702980
438 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1262400.v1.1.491

Sequence Viewer

Length: 438 bp
ATGTCAGCCCAACAAATCGCGAGCCACATAGAAGACGCCGAGATCTACCACGGCGAGGCCCTGTGCAGGCAGAAGTCCCGCGAGCTGCTCGACAAAATGTGCCTACCACGTGGCCTCCTTCCCTTGGACGACGTCGTCGAGTTTGGCTACAACCACACCTCCGGCTTCGTCTGGCTCAAGCAGAAGAAGAGAAAAGAGCACCGCTTCCGCGCCATTTCCAAAACGGTGTCGTATGACACGGAGGTGATGGGGTTCGTGGAGGAGCACCGGATGCGGCGGCTCTCCGGGGTCAAAAGCAAAGAGCTTCTGATTTGGGTCTCCATTTCGGATATCTACGTGGACCCTAATTCGGATCTGGATAAGATCACGTTCGCTAACGGTACCGGATCGTCTAGGTCGTTCCCCGCCACGGCGTTTGGGCTTGAAGGAGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

146

Amino Acids

16.52

Weight (kDa)

6.22

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 139 1e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016008)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46230
fragaria_vesca FvH4_3g05580
malus_domestica MD05G1284100.v1.1 MD10G1262400.v1.1
prunus_persica Prupe.4G080300_v2.0.a1
pyrus_communis pycom05g26320
rosa_chinensis RchiOBHm_Chr5g0014081
rosa_laevigata RLG00000032080
rosa_multiflora Rmu_sc0007767.1_g000010
rosa_roxburghii Rroxscaffold_1G00062540
rosa_rugosa Rorug05G0015500
rosa_samantha Rh5AG109500 Rh5BG106300 Rh5CG118000
rosa_wichuraiana Rw5G009560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AatII GACGTC 1 cut(s) 135
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 1 cut(s) 380
AccII CGCG 3 cut(s) 20, 81, 210
AciI CCGC 6 cut(s) 79, 202, 208, 274, 277, 405
AclWI GGATC 2 cut(s) 360, 394
AcvI CACGTG 1 cut(s) 110
AcyI GRCGYC 2 cut(s) 36, 132
AfaI GTAC 1 cut(s) 382
AfiI CCNNNNNNNGG 5 cut(s) 55, 66, 124, 349, 409
AgsI TTSAA 1 cut(s) 425
AleI CACNNNNGTG 1 cut(s) 242
AluBI AGCT 2 cut(s) 85, 304
AluI AGCT 2 cut(s) 85, 304
Alw21I GWGCWC 2 cut(s) 201, 267
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 2 cut(s) 360, 394
AoxI GGCC 2 cut(s) 57, 112
ApeKI GCWGC 1 cut(s) 85
Asp718I GGTACC 1 cut(s) 380
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 58, 340
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 1 cut(s) 256
AvaII GGWCC 1 cut(s) 340
BanI GGYRCC 1 cut(s) 380
BbrPI CACGTG 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 201, 267
BbvI GCAGC 1 cut(s) 72
BccI CCATC 1 cut(s) 241
BceAI ACGGC 2 cut(s) 67, 426
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 322
BfaI CTAG 1 cut(s) 393
BglII AGATCT 1 cut(s) 42
BisI GCNGC 3 cut(s) 86, 275, 278
BlsI GCNGC 3 cut(s) 87, 276, 279
Bme1390I CCNGG 1 cut(s) 286
Bme18I GGWCC 1 cut(s) 340
BmgT120I GGNCC 2 cut(s) 58, 340
BmiI GGNNCC 2 cut(s) 342, 382
BmrFI CCNGG 1 cut(s) 286
BmsI GCATC 1 cut(s) 261
BpiI GAAGAC 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 161
BpuMI CCSGG 1 cut(s) 286
BsaAI YACGTR 2 cut(s) 110, 337
BsaHI GRCGYC 2 cut(s) 36, 132
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 4 cut(s) 49, 123, 285, 408
BsaWI WCCGGW 2 cut(s) 267, 383
BsaXI ACNNNNNCTCC 4 cut(s) 99, 129, 143, 173
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 66, 124, 349, 409
BseDI CCNNGG 4 cut(s) 49, 123, 285, 408
BseGI GGATG 1 cut(s) 276
BseLI CCNNNNNNNGG 5 cut(s) 55, 66, 124, 349, 409
BseRI GAGGAG 1 cut(s) 275
BseXI GCAGC 1 cut(s) 72
BsgI GTGCAG 1 cut(s) 85
Bsh1236I CGCG 3 cut(s) 20, 81, 210
BshFI GGCC 2 cut(s) 59, 114
BshNI GGYRCC 1 cut(s) 380
BsiHKAI GWGCWC 2 cut(s) 201, 267
BsiSI CCGG 4 cut(s) 162, 268, 285, 384
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 5 cut(s) 55, 66, 124, 349, 409
BsmAI GTCTC 1 cut(s) 322
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 2 cut(s) 59, 114
Bso31I GGTCTC 1 cut(s) 322
Bsp1286I GDGCHC 2 cut(s) 201, 267
Bsp143I GATC 4 cut(s) 42, 352, 363, 386
Bsp68I TCGCGA 1 cut(s) 20
BspACI CCGC 6 cut(s) 79, 202, 208, 274, 277, 405
BspANI GGCC 2 cut(s) 59, 114
BspFNI CGCG 3 cut(s) 20, 81, 210
BspLI GGNNCC 2 cut(s) 342, 382
BspPI GGATC 2 cut(s) 360, 394
BspT107I GGYRCC 1 cut(s) 380
BspTNI GGTCTC 1 cut(s) 322
BssECI CCNNGG 4 cut(s) 49, 123, 285, 408
BssMI GATC 4 cut(s) 42, 352, 363, 386
BssNI GRCGYC 2 cut(s) 36, 132
BssT1I CCWWGG 1 cut(s) 123
Bst4CI ACNGT 2 cut(s) 226, 380
Bst6I CTCTTC 2 cut(s) 182, 426
BstACI GRCGYC 2 cut(s) 36, 132
BstAPI GCANNNNNTGC 1 cut(s) 271
BstBAI YACGTR 2 cut(s) 110, 337
BstC8I GCNNGC 3 cut(s) 22, 68, 83
BstDSI CCRYGG 2 cut(s) 49, 408
BstF5I GGATG 1 cut(s) 276
BstFNI CGCG 3 cut(s) 20, 81, 210
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 4 cut(s) 45, 355, 366, 389
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 4 cut(s) 42, 352, 363, 386
BstMWI GCNNNNNNNGC 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 284
BstUI CGCG 3 cut(s) 20, 81, 210
BstV1I GCAGC 1 cut(s) 72
BstV2I GAAGAC 1 cut(s) 39
BstX2I RGATCY 2 cut(s) 42, 352
BstYI RGATCY 2 cut(s) 42, 352
BsuRI GGCC 2 cut(s) 59, 114
BtgI CCRYGG 2 cut(s) 49, 408
BtsCI GGATG 1 cut(s) 276
BtuMI TCGCGA 1 cut(s) 20
Cac8I GCNNGC 3 cut(s) 22, 68, 83
CfoI GCGC 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 58, 340
CseI GACGC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 381
CspCI CAANNNNNGTGG 2 cut(s) 397, 432
CviQI GTAC 1 cut(s) 381
DpnI GATC 4 cut(s) 44, 354, 365, 388
DpnII GATC 4 cut(s) 42, 352, 363, 386
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
Eam1104I CTCTTC 2 cut(s) 182, 426
EarI CTCTTC 2 cut(s) 182, 426
Eco130I CCWWGG 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 322
Eco32I GATATC 1 cut(s) 331
Eco47I GGWCC 1 cut(s) 340
Eco72I CACGTG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 58
EcoRV GATATC 1 cut(s) 331
EcoT14I CCWWGG 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 123
FaiI YATR 2 cut(s) 29, 234
FaqI GGGAC 1 cut(s) 61
FauI CCCGC 2 cut(s) 86, 412
Fnu4HI GCNGC 3 cut(s) 86, 275, 278
FokI GGATG 1 cut(s) 283
Fsp4HI GCNGC 3 cut(s) 86, 275, 278
FspBI CTAG 1 cut(s) 393
GlaI GCGC 1 cut(s) 211
GluI GCNGC 3 cut(s) 86, 275, 278
HaeIII GGCC 2 cut(s) 59, 114
HapII CCGG 4 cut(s) 162, 268, 285, 384
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 212
Hin1I GRCGYC 2 cut(s) 36, 132
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HpaII CCGG 4 cut(s) 162, 268, 285, 384
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 3 cut(s) 309, 328, 352
Hpy188III TCNNGA 2 cut(s) 19, 356
Hpy8I GTNNAC 1 cut(s) 340
Hpy99I CGWCG 3 cut(s) 134, 137, 140
HpyAV CCTTC 2 cut(s) 128, 419
HpyCH4III ACNGT 2 cut(s) 226, 380
HpyCH4IV ACGT 4 cut(s) 109, 132, 336, 368
HpyCH4V TGCA 1 cut(s) 66
HpyF10VI GCNNNNNNNGC 1 cut(s) 271
HpySE526I ACGT 4 cut(s) 109, 132, 336, 368
Hsp92I GRCGYC 2 cut(s) 36, 132
HspAI GCGC 1 cut(s) 210
KpnI GGTACC 1 cut(s) 384
Kzo9I GATC 4 cut(s) 42, 352, 363, 386
LmnI GCTCC 1 cut(s) 262
LpnPI CCDG 8 cut(s) 52, 74, 157, 175, 281, 298, 341, 397
Lsp1109I GCAGC 1 cut(s) 72
LweI GCATC 1 cut(s) 261
MaeI CTAG 1 cut(s) 393
MaeII ACGT 4 cut(s) 109, 132, 336, 368
MalI GATC 4 cut(s) 44, 354, 365, 388
MboI GATC 4 cut(s) 42, 352, 363, 386
MboII GAAGA 3 cut(s) 44, 196, 199
MflI RGATCY 2 cut(s) 42, 352
MhlI GDGCHC 2 cut(s) 201, 267
MluCI AATT 1 cut(s) 346
MnlI CCTC 5 cut(s) 49, 125, 169, 235, 253
MslI CAYNNNNRTG 1 cut(s) 242
MspI CCGG 4 cut(s) 162, 268, 285, 384
MspR9I CCNGG 1 cut(s) 286
MvnI CGCG 3 cut(s) 20, 81, 210
MwoI GCNNNNNNNGC 1 cut(s) 271
NciI CCSGG 1 cut(s) 286
NdeII GATC 4 cut(s) 42, 352, 363, 386
NlaIV GGNNCC 2 cut(s) 342, 382
NmeAIII GCCGAG 1 cut(s) 64
NruI TCGCGA 1 cut(s) 20
OliI CACNNNNGTG 1 cut(s) 242
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 395
PflFI GACNNNGTC 2 cut(s) 131, 134
PkrI GCNGC 3 cut(s) 87, 276, 279
PmaCI CACGTG 1 cut(s) 110
PmlI CACGTG 1 cut(s) 110
Ppu21I YACGTR 2 cut(s) 110, 337
PspCI CACGTG 1 cut(s) 110
PspN4I GGNNCC 2 cut(s) 342, 382
PspPI GGNCC 2 cut(s) 58, 340
PsuI RGATCY 2 cut(s) 42, 352
PsyI GACNNNGTC 2 cut(s) 131, 134
RruI TCGCGA 1 cut(s) 20
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
RseI CAYNNNNRTG 1 cut(s) 242
SatI GCNGC 3 cut(s) 86, 275, 278
Sau3AI GATC 4 cut(s) 42, 352, 363, 386
Sau96I GGNCC 2 cut(s) 58, 340
ScrFI CCNGG 1 cut(s) 286
SduI GDGCHC 2 cut(s) 201, 267
SetI ASST 9 cut(s) 87, 112, 135, 161, 246, 306, 339, 371, 398
SfaNI GCATC 1 cut(s) 261
SinI GGWCC 1 cut(s) 340
SmiMI CAYNNNNRTG 1 cut(s) 242
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 1 cut(s) 346
SsiI CCGC 6 cut(s) 79, 202, 208, 274, 277, 405
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 284
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 226, 380
TaiI ACGT 4 cut(s) 112, 135, 339, 371
TaqI TCGA 2 cut(s) 90, 138
TasI AATT 1 cut(s) 346
TauI GCSGC 2 cut(s) 277, 280
TseI GCWGC 1 cut(s) 85
TspGWI ACGGA 1 cut(s) 254
Tth111I GACNNNGTC 2 cut(s) 131, 134
VpaK11BI GGWCC 1 cut(s) 340
XspI CTAG 1 cut(s) 393
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.