Rh5AG109500

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0016008
Physical Location & Seq
Reverse (-)
10034862 .. 10035329
468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG109500.1

Sequence Viewer

Length: 468 bp
ATGTCAAGCCAGCAACTAATCGCGACCCACAGAGACGGCGCCGAGATCCATCACGGCGAAGACGTATGCAAGCAAAAGTCCCACCAGCTTCTCGAGAAGATGCATCTGCCGTGCGGCCTCCTCCCTCTAGACGACGTCGTCGAGTTCGGCTACAACGCCACCTCGGGGTTCGTGTGGCTGAAGCAGAAGAAGCGAAAGGAGCACCGGTTCCATGCCGTGGGACGCACGGTTTCATACGATACTGAGGTCATGGGGTTTGTGGAGGAGCATCGGATGAGGCGTCTCAGCGGGGTCAAGAGTAAGGAGATTTTGATCTGGGTGTCCATTTCGGATATCTATGTGGATCCGAGTACGGATCCCGATAAGATCACTTTCGCCAACGGCACCGGAATCTCGAGGTCGTTCCCGATCACCGCGTTTCAACTTGAAGAAGGTGAAGATGGAAATGGTAATAATGGCAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

155

Amino Acids

17.48

Weight (kDa)

6.44

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 141 1.5e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016008)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46230
fragaria_vesca FvH4_3g05580
malus_domestica MD05G1284100.v1.1 MD10G1262400.v1.1
prunus_persica Prupe.4G080300_v2.0.a1
pyrus_communis pycom05g26320
rosa_chinensis RchiOBHm_Chr5g0014081
rosa_laevigata RLG00000032080
rosa_multiflora Rmu_sc0007767.1_g000010
rosa_roxburghii Rroxscaffold_1G00062540
rosa_rugosa Rorug05G0015500
rosa_samantha Rh5AG109500 Rh5BG106300 Rh5CG118000
rosa_wichuraiana Rw5G009560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 137
AatII GACGTC 1 cut(s) 138
AccB1I GGYRCC 2 cut(s) 38, 383
AccB7I CCANNNNNTGG 1 cut(s) 217
AccII CGCG 2 cut(s) 23, 416
AciI CCGC 3 cut(s) 114, 288, 414
AclWI GGATC 5 cut(s) 40, 338, 350, 351, 363
AcuI CTGAAG 1 cut(s) 200
AcyI GRCGYC 3 cut(s) 39, 135, 280
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 2 cut(s) 165, 217
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 2 cut(s) 422, 428
AloI GAACNNNNNNTCC 2 cut(s) 191, 223
AluBI AGCT 1 cut(s) 88
AluI AGCT 1 cut(s) 88
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 27, 287
AlwI GGATC 5 cut(s) 40, 338, 350, 351, 363
Ama87I CYCGRG 3 cut(s) 92, 163, 394
AoxI GGCC 1 cut(s) 115
AsiGI ACCGGT 1 cut(s) 204
AspLEI GCGC 1 cut(s) 41
AsuHPI GGTGA 2 cut(s) 403, 446
AvaI CYCGRG 3 cut(s) 92, 163, 394
BaeI ACNNNNGTAYC 2 cut(s) 231, 264
BamHI GGATCC 2 cut(s) 343, 355
BanI GGYRCC 2 cut(s) 38, 383
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 204
BccI CCATC 2 cut(s) 57, 434
BceAI ACGGC 5 cut(s) 52, 70, 94, 200, 397
BcoDI GTCTC 2 cut(s) 27, 287
BfaI CTAG 1 cut(s) 128
BfoI RGCGCY 1 cut(s) 42
BisI GCNGC 1 cut(s) 115
BlsI GCNGC 1 cut(s) 116
BmeT110I CYCGRG 3 cut(s) 92, 163, 394
BmiI GGNNCC 5 cut(s) 40, 209, 345, 357, 385
BmsI GCATC 3 cut(s) 90, 112, 277
BpiI GAAGAC 1 cut(s) 66
BsaBI GATNNNNATC 1 cut(s) 311
BsaHI GRCGYC 3 cut(s) 39, 135, 280
BsaJI CCNNGG 2 cut(s) 162, 216
BsaWI WCCGGW 2 cut(s) 204, 386
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 217
Bse118I RCCGGY 1 cut(s) 204
Bse8I GATNNNNATC 1 cut(s) 311
BseDI CCNNGG 2 cut(s) 162, 216
BseGI GGATG 1 cut(s) 279
BseJI GATNNNNATC 1 cut(s) 311
BseLI CCNNNNNNNGG 2 cut(s) 165, 217
BseMII CTCAG 2 cut(s) 234, 298
BseRI GAGGAG 2 cut(s) 110, 278
Bsh1236I CGCG 2 cut(s) 23, 416
BshFI GGCC 1 cut(s) 117
BshNI GGYRCC 2 cut(s) 38, 383
BshTI ACCGGT 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 3 cut(s) 92, 163, 394
BsiSI CCGG 2 cut(s) 205, 387
BslFI GGGAC 2 cut(s) 64, 234
BslI CCNNNNNNNGG 2 cut(s) 165, 217
BsmAI GTCTC 2 cut(s) 27, 287
BsmBI CGTCTC 2 cut(s) 27, 287
BsmFI GGGAC 2 cut(s) 64, 234
BsnI GGCC 1 cut(s) 117
BsoBI CYCGRG 3 cut(s) 92, 163, 394
Bsp1286I GDGCHC 1 cut(s) 204
Bsp143I GATC 6 cut(s) 45, 312, 343, 355, 366, 408
Bsp68I TCGCGA 1 cut(s) 23
BspACI CCGC 3 cut(s) 114, 288, 414
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 2 cut(s) 235, 297
BspFNI CGCG 2 cut(s) 23, 416
BspLI GGNNCC 5 cut(s) 40, 209, 345, 357, 385
BspPI GGATC 5 cut(s) 40, 338, 350, 351, 363
BspT107I GGYRCC 2 cut(s) 38, 383
BsrFI RCCGGY 1 cut(s) 204
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 2 cut(s) 162, 216
BssMI GATC 6 cut(s) 45, 312, 343, 355, 366, 408
BssNI GRCGYC 3 cut(s) 39, 135, 280
Bst4CI ACNGT 1 cut(s) 229
BstACI GRCGYC 3 cut(s) 39, 135, 280
BstC8I GCNNGC 2 cut(s) 11, 71
BstDEI CTNAG 2 cut(s) 243, 284
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 1 cut(s) 279
BstFNI CGCG 2 cut(s) 23, 416
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 6 cut(s) 48, 315, 346, 358, 369, 411
BstMAI GTCTC 2 cut(s) 27, 287
BstMBI GATC 6 cut(s) 45, 312, 343, 355, 366, 408
BstMWI GCNNNNNNNGC 2 cut(s) 190, 199
BstUI CGCG 2 cut(s) 23, 416
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 3 cut(s) 45, 343, 355
BstYI RGATCY 3 cut(s) 45, 343, 355
BsuRI GGCC 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 1 cut(s) 279
BtuMI TCGCGA 1 cut(s) 23
Cac8I GCNNGC 2 cut(s) 11, 71
CfoI GCGC 1 cut(s) 41
Cfr10I RCCGGY 1 cut(s) 204
CseI GACGC 2 cut(s) 231, 269
Csp6I GTAC 1 cut(s) 351
CspAI ACCGGT 1 cut(s) 204
CviAII CATG 2 cut(s) 212, 250
CviJI RGCY 5 cut(s) 9, 88, 117, 150, 178
CviKI_1 RGCY 5 cut(s) 9, 88, 117, 150, 178
CviQI GTAC 1 cut(s) 351
DdeI CTNAG 2 cut(s) 243, 284
DinI GGCGCC 1 cut(s) 40
DpnI GATC 6 cut(s) 47, 314, 345, 357, 368, 410
DpnII GATC 6 cut(s) 45, 312, 343, 355, 366, 408
DrdI GACNNNNNNGTC 1 cut(s) 137
DseDI GACNNNNNNGTC 1 cut(s) 137
Eco32I GATATC 1 cut(s) 334
Eco57I CTGAAG 1 cut(s) 200
Eco88I CYCGRG 3 cut(s) 92, 163, 394
EcoRV GATATC 1 cut(s) 334
EcoT22I ATGCAT 1 cut(s) 105
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
Esp3I CGTCTC 2 cut(s) 27, 287
FaeI CATG 2 cut(s) 215, 253
FaiI YATR 5 cut(s) 67, 213, 235, 251, 339
FaqI GGGAC 2 cut(s) 64, 234
FatI CATG 2 cut(s) 211, 249
FauI CCCGC 1 cut(s) 281
Fnu4HI GCNGC 1 cut(s) 115
FokI GGATG 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 115
FspBI CTAG 1 cut(s) 128
GlaI GCGC 1 cut(s) 40
GluI GCNGC 1 cut(s) 115
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 1 cut(s) 117
HapII CCGG 2 cut(s) 205, 387
HgaI GACGC 2 cut(s) 231, 269
HhaI GCGC 1 cut(s) 41
Hin1I GRCGYC 3 cut(s) 39, 135, 280
Hin1II CATG 2 cut(s) 215, 253
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HinfI GANTC 1 cut(s) 390
HpaII CCGG 2 cut(s) 205, 387
HphI GGTGA 2 cut(s) 403, 446
Hpy188I TCNGA 3 cut(s) 273, 331, 348
Hpy188III TCNNGA 8 cut(s) 22, 92, 94, 128, 295, 359, 394, 406
Hpy99I CGWCG 3 cut(s) 137, 140, 143
HpyAV CCTTC 1 cut(s) 425
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 2 cut(s) 63, 135
HpyCH4V TGCA 2 cut(s) 69, 103
HpyF10VI GCNNNNNNNGC 2 cut(s) 190, 199
HpyF3I CTNAG 2 cut(s) 243, 284
HpySE526I ACGT 2 cut(s) 63, 135
Hsp92I GRCGYC 3 cut(s) 39, 135, 280
Hsp92II CATG 2 cut(s) 215, 253
HspAI GCGC 1 cut(s) 39
KasI GGCGCC 1 cut(s) 38
Kzo9I GATC 6 cut(s) 45, 312, 343, 355, 366, 408
LmnI GCTCC 2 cut(s) 199, 265
LpnPI CCDG 5 cut(s) 23, 98, 218, 301, 400
LweI GCATC 3 cut(s) 90, 112, 277
MaeI CTAG 1 cut(s) 128
MaeII ACGT 2 cut(s) 63, 135
MalI GATC 6 cut(s) 47, 314, 345, 357, 368, 410
MboI GATC 6 cut(s) 45, 312, 343, 355, 366, 408
MboII GAAGA 5 cut(s) 71, 109, 199, 440, 449
MflI RGATCY 3 cut(s) 45, 343, 355
MhlI GDGCHC 1 cut(s) 204
Mly113I GGCGCC 1 cut(s) 39
MnlI CCTC 8 cut(s) 128, 131, 135, 172, 238, 256, 270, 390
Mph1103I ATGCAT 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 2 cut(s) 205, 387
MvnI CGCG 2 cut(s) 23, 416
MwoI GCNNNNNNNGC 2 cut(s) 190, 199
NarI GGCGCC 1 cut(s) 39
NdeII GATC 6 cut(s) 45, 312, 343, 355, 366, 408
NlaIII CATG 2 cut(s) 215, 253
NlaIV GGNNCC 5 cut(s) 40, 209, 345, 357, 385
NmeAIII GCCGAG 1 cut(s) 67
NruI TCGCGA 1 cut(s) 23
NsiI ATGCAT 1 cut(s) 105
PaeR7I CTCGAG 2 cut(s) 92, 394
PcsI WCGNNNNNNNCGW 3 cut(s) 60, 138, 277
PfeI GAWTC 1 cut(s) 390
PflFI GACNNNGTC 2 cut(s) 134, 137
PflMI CCANNNNNTGG 1 cut(s) 217
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 1 cut(s) 116
PluTI GGCGCC 1 cut(s) 42
PspN4I GGNNCC 5 cut(s) 40, 209, 345, 357, 385
PsuI RGATCY 3 cut(s) 45, 343, 355
PsyI GACNNNGTC 2 cut(s) 134, 137
RruI TCGCGA 1 cut(s) 23
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
SatI GCNGC 1 cut(s) 115
Sau3AI GATC 6 cut(s) 45, 312, 343, 355, 366, 408
SduI GDGCHC 1 cut(s) 204
SetI ASST 7 cut(s) 66, 90, 138, 164, 249, 401, 436
SfaNI GCATC 3 cut(s) 90, 112, 277
SfoI GGCGCC 1 cut(s) 40
Sfr274I CTCGAG 2 cut(s) 92, 394
SlaI CTCGAG 2 cut(s) 92, 394
SmlI CTYRAG 2 cut(s) 92, 394
SmoI CTYRAG 2 cut(s) 92, 394
SsiI CCGC 3 cut(s) 114, 288, 414
SspDI GGCGCC 1 cut(s) 38
SspMI CTAG 1 cut(s) 128
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 2 cut(s) 66, 138
TaqI TCGA 3 cut(s) 93, 141, 395
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 1 cut(s) 390
TspDTI ATGAA 1 cut(s) 222
TspGWI ACGGA 1 cut(s) 368
Tth111I GACNNNGTC 2 cut(s) 134, 137
Van91I CCANNNNNTGG 1 cut(s) 217
XbaI TCTAGA 1 cut(s) 127
XhoI CTCGAG 2 cut(s) 92, 394
XspI CTAG 1 cut(s) 128
ZraI GACGTC 1 cut(s) 136
Zsp2I ATGCAT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.