Rorug05G0015500

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0016008
Physical Location & Seq
Forward (+)
1048365 .. 1051500
3136 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0015500.1

Sequence Viewer

Length: 2241 bp
ATGGCAGAAAATAGCAGCATAAATCCCAAACACCTATATCAAAGCCTAAAGAATGGTGACACAGCCAAAGTGATGGAATATTGTTATGATAAAACTAGATCTGATGATGAAAGGTTGAAGCTCATTCCAACAGTGCACGATGATACAATCCTCCATTTAGCAATTAACATGGGGCAGCAAGACATAGCAAAAGAAATCGTCAAAAGATGTTCCGGGGGTTCTAATGATAAGCTACTTATGAAGAAGAATGCCCTCGGAAACACAGTTCTGCACGAGGCTGCAGCAACAAGCATGACAAGCCTTGTGGAGGAGTTGTTAAGAAAGGCATCAGACTTGTTGTCTATTTCGAACAACAAAAATGAAATGCCTCTCTATACGGCAGCTTACTATGGCCAAACCGAAATGTTCAAGTTGTTGGCGGACGAAGTAGACAAAAACCAGTCTGGCAATCTTGATCTTGATCTTCATTTGAGACCTAGGGGTCGTAAGACGAACCCCAATAAAGTGCGGACTACCATTCTTCATGCTGCAATACATGCCGAGTTCTTTGAACTTGCACTTCTGATTGCAAAAAGGTATACGCAACTAGTTAAGAAAAAGGACGAAAATCAGCTGACAGGCCTTCAGCTGCTTTCATGTAATCCTTCAGCTTTCAGGAGTGGAACCAAATATGGACCGATCAAGAGGTTCATTTATTCCTGTATGCTTTCTTTCAATTTTCCTGAACTCATTTACCAATTGATCACATTTAAATTAGTGCTCCAAATTAATGAGAATGTGATAATGGATAGAAATGGCAGCCACGAACACGAAGAGGCTGATGAATACATTTTCAGCGATCTAGGTGACGAGGAAAATACAGCTTGCTTCAAGATGCCTCAGAGTCAAACATGTACATATATGAATAACCGCTGCACCATTGCTTTACGGCAAGCCATCTCTTCTGTTAATAGAGCGGTTTGGAAAATTCTTCAAGCTTGGGGCACAATGAAGAGGATCTATGGGGACAAGAAGAGGCATGAATCAGCCTTCAAACTAGCCAAGTTACTAATTGCAGCAGACTTTTCATGGGACAGCATAGGAGTACAAGATGAAATCGCTAGCTCAAAGGTCACCGCTGCTACAAATGATGCTCCCTCTTTGGATCGCAAAGCTGATATTGATTGGATAGAAGCCCTTTTAGGTGCAGAAGCCTTAAGTGGCAGCGGTGCTGTTAGTGGCGGCCCAGAAATAGCAGCTTCATCCATGTCAGAACTTCATTCACCTCCAACGCCATTGCTCCTTGCAACAAGGCACGGAATACTAGAAATTGTGGATGAGATACTTAAAGTCTACCCTCTGGCAGTTGAGCATATAAGTGAAATGGGGCAAAACTTGCTGCATGTTGCTGTTCTGCACCGCCAGTTGGACGTCTTTCAACGAGTCATTAGGATGGAATCACCCAAGTCAAGGTTGGTTCGACGTATCGACAACAATGGATTTACAATCTTGCACCATGTTGGAAACATGGCCTTTTACAAAGGAAGCACACAGCCTGGTCCTGCACTCCAGTTGCAAGAAGAGTTAGAATGGTTTGAGATTGTGGAGCAGGTGATTCCACCCCATTATGCGATGCAACTTGCCTGGAACGAAAAGGAACAGAAAAACGAGACCCCCAAACAGTACTTTCGTTGCACACATGGCGAGCTTCTCAAGGAGGCCCAAGATTGGATAAAGCGGACCGCCGAGTCGTGCTCGACGGTGGCAGGACTAATGGCCACCGTCGCTTTCGCCGCCGCCTTCACCGTCCCTGGAGGCAACGACGAGAAAAGCGGCACTCCACTCCTTCTTCACAGCCCTTTCTTCTTAGTCTTCATCATCACCGACGCTTTCTCCCTAGCCAGCTCGCTCACGTCACTCGTCATGTTCCTCTCGATTCTGACGTCACCGTACGAGATCGACGACTTCCGCTACTCTCTCCCTCGGAAGCTTATCCTCGGCTTCACCTTCCTCTTCTTCTCGGTGGCGGTGACCATGCTGGCCTTTGCTTCCACACTGATGCTGACAATTTCGACAAAGAAGAGCCTGACAACAACTTTTATATACTGCGTTGCGTTTCTTCCGGTCACTATGTTCGCGCTATTGCAGTTCCCTTTGTACGTGGCCTTCAAAACGACTCTCCTGTACTCTGTACAGGCCATCCAAAGCGTTCTTCCGTGGCATCTCATCTCTGTTGGTTTTTCAAAACTCAAAAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

746

Amino Acids

83.68

Weight (kDa)

6.73

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 51 - 147 1.9e-09 Ankyrin repeats (3 copies)
PGG PF13962 569 - 681 1e-24 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016008)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46230
fragaria_vesca FvH4_3g05580
malus_domestica MD05G1284100.v1.1 MD10G1262400.v1.1
prunus_persica Prupe.4G080300_v2.0.a1
pyrus_communis pycom05g26320
rosa_chinensis RchiOBHm_Chr5g0014081
rosa_laevigata RLG00000032080
rosa_multiflora Rmu_sc0007767.1_g000010
rosa_roxburghii Rroxscaffold_1G00062540
rosa_rugosa Rorug05G0015500
rosa_samantha Rh5AG109500 Rh5BG106300 Rh5CG118000
rosa_wichuraiana Rw5G009560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1579
AasI GACNNNNNNGTC 2 cut(s) 480, 1726
AatII GACGTC 2 cut(s) 1413, 1925
Acc36I ACCTGC 1 cut(s) 1579
AccBSI CCGCTC 1 cut(s) 956
AccI GTMKAC 3 cut(s) 429, 578, 1332
AccII CGCG 1 cut(s) 2117
AclWI GGATC 2 cut(s) 1004, 1152
AcoI YGGCCR 2 cut(s) 391, 1755
AcsI RAATTY 1 cut(s) 966
AcuI CTGAAG 2 cut(s) 608, 630
AcyI GRCGYC 2 cut(s) 1410, 1922
AfaI GTAC 7 cut(s) 895, 1086, 1664, 1931, 2138, 2165, 2172
AfiI CCNNNNNNNGG 4 cut(s) 307, 1405, 1449, 1707
AflII CTTAAG 1 cut(s) 1195
AflIII ACRYGT 1 cut(s) 890
AhdI GACNNNNNGTC 1 cut(s) 337
AhlI ACTAGT 1 cut(s) 586
AjiI CACGTC 1 cut(s) 1893
AjnI CCWGG 3 cut(s) 1534, 1622, 1789
AjuI GAANNNNNNNTTGG 2 cut(s) 2175, 2207
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
Alw21I GWGCWC 3 cut(s) 138, 762, 1736
Alw26I GTCTC 2 cut(s) 466, 1643
Alw44I GTGCAC 1 cut(s) 134
AlwI GGATC 2 cut(s) 1004, 1152
AoxI GGCC 9 cut(s) 391, 619, 1222, 1509, 1698, 1755, 2019, 2142, 2175
ApaLI GTGCAC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 966
AseI ATTAAT 1 cut(s) 768
AspA2I CCTAGG 1 cut(s) 476
AspLEI GCGC 1 cut(s) 2119
AspS9I GGNCC 5 cut(s) 674, 1223, 1538, 1699, 1719
AsuC2I CCSGG 1 cut(s) 214
AsuII TTCGAA 1 cut(s) 347
AsuNHI GCTAGC 1 cut(s) 1100
AvaII GGWCC 3 cut(s) 674, 1538, 1719
AvrII CCTAGG 1 cut(s) 476
BaeGI GKGCMC 2 cut(s) 138, 986
BalI TGGCCA 2 cut(s) 393, 1757
BarI GAAGNNNNNNTAC 2 cut(s) 2129, 2161
BauI CACGAG 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 1843
Bbv12I GWGCWC 3 cut(s) 138, 762, 1736
BccI CCATC 4 cut(s) 67, 944, 1426, 2186
BceAI ACGGC 2 cut(s) 393, 944
BciT130I CCWGG 3 cut(s) 1536, 1624, 1791
BclI TGATCA 1 cut(s) 741
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 2 cut(s) 466, 1643
BcuI ACTAGT 1 cut(s) 586
BfaI CTAG 8 cut(s) 96, 477, 587, 842, 1037, 1101, 1304, 1877
BfmI CTRYAG 1 cut(s) 279
BfrI CTTAAG 1 cut(s) 1195
BfuAI ACCTGC 1 cut(s) 1579
BglII AGATCT 1 cut(s) 98
BlnI CCTAGG 1 cut(s) 476
BmcAI AGTACT 1 cut(s) 1664
Bme1390I CCNGG 4 cut(s) 214, 1536, 1624, 1791
Bme18I GGWCC 3 cut(s) 674, 1538, 1719
BmeRI GACNNNNNGTC 1 cut(s) 337
BmgBI CACGTC 1 cut(s) 1893
BmgT120I GGNCC 5 cut(s) 674, 1223, 1538, 1699, 1719
BmiI GGNNCC 1 cut(s) 664
BmrFI CCNGG 4 cut(s) 214, 1536, 1624, 1791
BmsI GCATC 6 cut(s) 335, 864, 1120, 1602, 2028, 2209
BmtI GCTAGC 1 cut(s) 1104
BpiI GAAGAC 1 cut(s) 1843
BplI GAGNNNNNCTC 2 cut(s) 1718, 1750
BpmI CTGGAG 2 cut(s) 1532, 1812
Bpu14I TTCGAA 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 1676
BpuMI CCSGG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 2140
BsaBI GATNNNNATC 1 cut(s) 459
BsaHI GRCGYC 2 cut(s) 1410, 1922
BsaI GGTCTC 2 cut(s) 466, 1643
BsaJI CCNNGG 7 cut(s) 213, 253, 476, 1789, 1961, 1975, 2195
BsaWI WCCGGW 1 cut(s) 2101
BsaXI ACNNNNNCTCC 4 cut(s) 299, 329, 1856, 1886
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 1405, 1449, 1707
Bse1I ACTGG 3 cut(s) 439, 1402, 1549
Bse3DI GCAATG 2 cut(s) 918, 1274
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 3 cut(s) 1536, 1624, 1791
BseDI CCNNGG 7 cut(s) 213, 253, 476, 1789, 1961, 1975, 2195
BseGI GGATG 4 cut(s) 1241, 1321, 1437, 2178
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 4 cut(s) 307, 1405, 1449, 1707
BseMI GCAATG 2 cut(s) 918, 1274
BseMII CTCAG 1 cut(s) 893
BseNI ACTGG 3 cut(s) 439, 1402, 1549
BseRI GAGGAG 1 cut(s) 323
BseSI GKGCMC 2 cut(s) 138, 986
BsgI GTGCAG 5 cut(s) 254, 898, 1206, 1379, 1527
Bsh1236I CGCG 1 cut(s) 2117
BshFI GGCC 9 cut(s) 393, 621, 1224, 1511, 1700, 1757, 2021, 2144, 2177
BsiHKAI GWGCWC 3 cut(s) 138, 762, 1736
BsiSI CCGG 2 cut(s) 213, 2102
BsiWI CGTACG 1 cut(s) 1929
BslFI GGGAC 3 cut(s) 1019, 1085, 1772
BslI CCNNNNNNNGG 4 cut(s) 307, 1405, 1449, 1707
BsmAI GTCTC 2 cut(s) 466, 1643
BsmFI GGGAC 3 cut(s) 1019, 1085, 1772
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 9 cut(s) 393, 621, 1224, 1511, 1700, 1757, 2021, 2144, 2177
Bso31I GGTCTC 2 cut(s) 466, 1643
Bsp119I TTCGAA 1 cut(s) 347
Bsp1286I GDGCHC 4 cut(s) 138, 762, 986, 1736
Bsp1407I TGTACA 2 cut(s) 893, 2170
Bsp143I GATC 9 cut(s) 98, 454, 460, 678, 741, 838, 996, 1144, 1935
BspANI GGCC 9 cut(s) 393, 621, 1224, 1511, 1700, 1757, 2021, 2144, 2177
BspCNI CTCAG 1 cut(s) 892
BspFNI CGCG 1 cut(s) 2117
BspLI GGNNCC 1 cut(s) 664
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 1 cut(s) 1579
BspOI GCTAGC 1 cut(s) 1104
BspPI GGATC 2 cut(s) 1004, 1152
BspQI GCTCTTC 1 cut(s) 2054
BspT104I TTCGAA 1 cut(s) 347
BspTI CTTAAG 1 cut(s) 1195
BspTNI GGTCTC 2 cut(s) 466, 1643
BsrBI CCGCTC 1 cut(s) 956
BsrDI GCAATG 2 cut(s) 918, 1274
BsrGI TGTACA 2 cut(s) 893, 2170
BsrI ACTGG 3 cut(s) 439, 1402, 1549
BssECI CCNNGG 7 cut(s) 213, 253, 476, 1789, 1961, 1975, 2195
BssMI GATC 9 cut(s) 98, 454, 460, 678, 741, 838, 996, 1144, 1935
BssNAI GTATAC 1 cut(s) 579
BssNI GRCGYC 2 cut(s) 1410, 1922
BssSI CACGAG 1 cut(s) 272
BssT1I CCWWGG 1 cut(s) 476
Bst1107I GTATAC 1 cut(s) 579
Bst2BI CACGAG 1 cut(s) 272
Bst2UI CCWGG 3 cut(s) 1536, 1624, 1791
Bst4CI ACNGT 7 cut(s) 133, 265, 1662, 1741, 1762, 1786, 1929
Bst6I CTCTTC 7 cut(s) 807, 946, 986, 1007, 1554, 1997, 2054
BstACI GRCGYC 2 cut(s) 1410, 1922
BstAFI CTTAAG 1 cut(s) 1195
BstAPI GCANNNNNTGC 2 cut(s) 536, 1375
BstAUI TGTACA 2 cut(s) 893, 2170
BstBAI YACGTR 1 cut(s) 2140
BstBI TTCGAA 1 cut(s) 347
BstC8I GCNNGC 7 cut(s) 865, 933, 1102, 1685, 1882, 1886, 2019
BstDEI CTNAG 2 cut(s) 879, 1846
BstDSI CCRYGG 1 cut(s) 2195
BstEII GGTNACC 2 cut(s) 1111, 2008
BstENI CCTNNNNNAGG 1 cut(s) 305
BstF5I GGATG 4 cut(s) 1241, 1321, 1437, 2178
BstFNI CGCG 1 cut(s) 2117
BstHHI GCGC 1 cut(s) 2119
BstKTI GATC 9 cut(s) 101, 457, 463, 681, 744, 841, 999, 1147, 1938
BstMAI GTCTC 2 cut(s) 466, 1643
BstMBI GATC 9 cut(s) 98, 454, 460, 678, 741, 838, 996, 1144, 1935
BstMWI GCNNNNNNNGC 6 cut(s) 297, 536, 1375, 1680, 1763, 1772
BstNI CCWGG 3 cut(s) 1536, 1624, 1791
BstNSI RCATGY 3 cut(s) 539, 894, 1385
BstPI GGTNACC 2 cut(s) 1111, 2008
BstSCI CCNGG 4 cut(s) 212, 1534, 1622, 1789
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 2 cut(s) 138, 986
BstUI CGCG 1 cut(s) 2117
BstV2I GAAGAC 1 cut(s) 1843
BstX2I RGATCY 2 cut(s) 98, 996
BstXI CCANNNNNNTGG 1 cut(s) 73
BstYI RGATCY 2 cut(s) 98, 996
BstZ17I GTATAC 1 cut(s) 579
BsuRI GGCC 9 cut(s) 393, 621, 1224, 1511, 1700, 1757, 2021, 2144, 2177
BtgI CCRYGG 1 cut(s) 2195
BtgZI GCGATG 1 cut(s) 1625
BtrI CACGTC 1 cut(s) 1893
BtsCI GGATG 4 cut(s) 1241, 1321, 1437, 2178
BtsIMutI CAGTG 2 cut(s) 138, 2033
BveI ACCTGC 1 cut(s) 1579
Cac8I GCNNGC 7 cut(s) 865, 933, 1102, 1685, 1882, 1886, 2019
CfoI GCGC 1 cut(s) 2119
Cfr13I GGNCC 5 cut(s) 674, 1223, 1538, 1699, 1719
CpoI CGGWCCG 1 cut(s) 1719
CseI GACGC 1 cut(s) 1874
Csp6I GTAC 7 cut(s) 894, 1085, 1663, 1930, 2137, 2164, 2171
CspCI CAANNNNNGTGG 2 cut(s) 285, 320
CspI CGGWCCG 1 cut(s) 1719
CviQI GTAC 7 cut(s) 894, 1085, 1663, 1930, 2137, 2164, 2171
DdeI CTNAG 2 cut(s) 879, 1846
DpnI GATC 9 cut(s) 100, 456, 462, 680, 743, 840, 998, 1146, 1937
DpnII GATC 9 cut(s) 98, 454, 460, 678, 741, 838, 996, 1144, 1935
DraI TTTAAA 1 cut(s) 751
DrdI GACNNNNNNGTC 2 cut(s) 480, 1726
DriI GACNNNNNGTC 1 cut(s) 337
DseDI GACNNNNNNGTC 2 cut(s) 480, 1726
EaeI YGGCCR 2 cut(s) 391, 1755
Eam1104I CTCTTC 7 cut(s) 807, 946, 986, 1007, 1554, 1997, 2054
Eam1105I GACNNNNNGTC 1 cut(s) 337
EarI CTCTTC 7 cut(s) 807, 946, 986, 1007, 1554, 1997, 2054
EciI GGCGGA 1 cut(s) 434
Eco130I CCWWGG 1 cut(s) 476
Eco147I AGGCCT 1 cut(s) 621
Eco31I GGTCTC 2 cut(s) 466, 1643
Eco47I GGWCC 3 cut(s) 674, 1538, 1719
Eco57I CTGAAG 2 cut(s) 608, 630
Eco91I GGTNACC 2 cut(s) 1111, 2008
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO65I GGTNACC 2 cut(s) 1111, 2008
EcoRII CCWGG 3 cut(s) 1534, 1622, 1789
EcoT14I CCWWGG 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 476
FaqI GGGAC 3 cut(s) 1019, 1085, 1772
FbaI TGATCA 1 cut(s) 741
FblI GTMKAC 3 cut(s) 429, 578, 1332
FokI GGATG 4 cut(s) 1228, 1328, 1444, 2165
FspBI CTAG 8 cut(s) 96, 477, 587, 842, 1037, 1101, 1304, 1877
GlaI GCGC 1 cut(s) 2118
GsuI CTGGAG 2 cut(s) 1532, 1812
HaeIII GGCC 9 cut(s) 393, 621, 1224, 1511, 1700, 1757, 2021, 2144, 2177
HapII CCGG 2 cut(s) 213, 2102
HgaI GACGC 1 cut(s) 1874
HhaI GCGC 1 cut(s) 2119
Hin1I GRCGYC 2 cut(s) 1410, 1922
Hin6I GCGC 1 cut(s) 2117
HinP1I GCGC 1 cut(s) 2117
HindIII AAGCTT 2 cut(s) 975, 1967
HinfI GANTC 8 cut(s) 883, 1022, 1422, 1436, 1594, 1727, 1915, 2155
HpaII CCGG 2 cut(s) 213, 2102
Hpy166II GTNNAC 4 cut(s) 136, 430, 579, 1333
Hpy188I TCNGA 8 cut(s) 103, 257, 331, 564, 882, 1252, 1920, 1965
Hpy188III TCNNGA 7 cut(s) 452, 458, 655, 682, 722, 871, 1912
Hpy8I GTNNAC 4 cut(s) 136, 430, 579, 1333
Hpy99I CGWCG 6 cut(s) 1464, 1741, 1766, 1805, 1868, 1943
HpyAV CCTTC 7 cut(s) 632, 654, 1039, 1789, 1835, 1996, 2155
HpyCH4III ACNGT 7 cut(s) 133, 265, 1662, 1741, 1762, 1786, 1929
HpyCH4IV ACGT 5 cut(s) 1410, 1462, 1892, 1922, 2139
HpyF10VI GCNNNNNNNGC 6 cut(s) 297, 536, 1375, 1680, 1763, 1772
HpyF3I CTNAG 2 cut(s) 879, 1846
HpySE526I ACGT 5 cut(s) 1410, 1462, 1892, 1922, 2139
Hsp92I GRCGYC 2 cut(s) 1410, 1922
HspAI GCGC 1 cut(s) 2117
Ksp22I TGATCA 1 cut(s) 741
Kzo9I GATC 9 cut(s) 98, 454, 460, 678, 741, 838, 996, 1144, 1935
LguI GCTCTTC 1 cut(s) 2054
LmnI GCTCC 4 cut(s) 765, 1138, 1284, 1585
LweI GCATC 6 cut(s) 335, 864, 1120, 1602, 2028, 2209
MaeI CTAG 8 cut(s) 96, 477, 587, 842, 1037, 1101, 1304, 1877
MaeII ACGT 5 cut(s) 1410, 1462, 1892, 1922, 2139
MaeIII GTNAC 8 cut(s) 56, 845, 1044, 1111, 1893, 1923, 2008, 2104
MalI GATC 9 cut(s) 100, 456, 462, 680, 743, 840, 998, 1146, 1937
MbiI CCGCTC 1 cut(s) 956
MboI GATC 9 cut(s) 98, 454, 460, 678, 741, 838, 996, 1144, 1935
MfeI CAATTG 1 cut(s) 737
MflI RGATCY 2 cut(s) 98, 996
MhlI GDGCHC 4 cut(s) 138, 762, 986, 1736
MlsI TGGCCA 2 cut(s) 393, 1757
MluNI TGGCCA 2 cut(s) 393, 1757
MlyI GAGTC 4 cut(s) 892, 1431, 1736, 2149
MmeI TCCRAC 4 cut(s) 152, 1292, 1386, 1480
Mox20I TGGCCA 2 cut(s) 393, 1757
MscI TGGCCA 2 cut(s) 393, 1757
MseI TTAA 9 cut(s) 165, 317, 591, 750, 768, 948, 1196, 1326, 2239
MslI CAYNNNNRTG 3 cut(s) 1356, 1430, 2036
Msp20I TGGCCA 2 cut(s) 393, 1757
MspA1I CMGCKG 5 cut(s) 613, 628, 912, 1118, 1206
MspCI CTTAAG 1 cut(s) 1195
MspI CCGG 2 cut(s) 213, 2102
MspR9I CCNGG 4 cut(s) 214, 1536, 1624, 1791
MunI CAATTG 1 cut(s) 737
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 3 cut(s) 1536, 1624, 1791
MvnI CGCG 1 cut(s) 2117
MwoI GCNNNNNNNGC 6 cut(s) 297, 536, 1375, 1680, 1763, 1772
NciI CCSGG 1 cut(s) 214
NdeII GATC 9 cut(s) 98, 454, 460, 678, 741, 838, 996, 1144, 1935
NheI GCTAGC 1 cut(s) 1100
NlaIV GGNNCC 1 cut(s) 664
NmeAIII GCCGAG 3 cut(s) 565, 1750, 1956
NmuCI GTSAC 7 cut(s) 56, 845, 1111, 1893, 1923, 2008, 2104
NspI RCATGY 3 cut(s) 539, 894, 1385
NspV TTCGAA 1 cut(s) 347
PaqCI CACCTGC 1 cut(s) 1579
PceI AGGCCT 1 cut(s) 621
PciI ACATGT 1 cut(s) 890
PciSI GCTCTTC 1 cut(s) 2054
PcsI WCGNNNNNNNCGW 2 cut(s) 1919, 1938
PctI GAATGC 1 cut(s) 253
PfeI GAWTC 4 cut(s) 1022, 1436, 1594, 1915
Pfl23II CGTACG 1 cut(s) 1929
PleI GAGTC 4 cut(s) 891, 1430, 1735, 2149
PpsI GAGTC 4 cut(s) 891, 1430, 1735, 2149
Ppu21I YACGTR 1 cut(s) 2140
PscI ACATGT 1 cut(s) 890
PshBI ATTAAT 1 cut(s) 768
Psp6I CCWGG 3 cut(s) 1534, 1622, 1789
PspEI GGTNACC 2 cut(s) 1111, 2008
PspGI CCWGG 3 cut(s) 1534, 1622, 1789
PspLI CGTACG 1 cut(s) 1929
PspN4I GGNNCC 1 cut(s) 664
PspPI GGNCC 5 cut(s) 674, 1223, 1538, 1699, 1719
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 2 cut(s) 98, 996
PvuII CAGCTG 2 cut(s) 613, 628
RsaI GTAC 7 cut(s) 895, 1086, 1664, 1931, 2138, 2165, 2172
RsaNI GTAC 7 cut(s) 894, 1085, 1663, 1930, 2137, 2164, 2171
RseI CAYNNNNRTG 3 cut(s) 1356, 1430, 2036
Rsr2I CGGWCCG 1 cut(s) 1719
RsrII CGGWCCG 1 cut(s) 1719
SapI GCTCTTC 1 cut(s) 2054
SaqAI TTAA 9 cut(s) 165, 317, 591, 750, 768, 948, 1196, 1326, 2239
Sau3AI GATC 9 cut(s) 98, 454, 460, 678, 741, 838, 996, 1144, 1935
Sau96I GGNCC 5 cut(s) 674, 1223, 1538, 1699, 1719
ScaI AGTACT 1 cut(s) 1664
SchI GAGTC 4 cut(s) 892, 1431, 1736, 2149
ScrFI CCNGG 4 cut(s) 214, 1536, 1624, 1791
SduI GDGCHC 4 cut(s) 138, 762, 986, 1736
SfaNI GCATC 6 cut(s) 335, 864, 1120, 1602, 2028, 2209
SfcI CTRYAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 347
SinI GGWCC 3 cut(s) 674, 1538, 1719
SmiI ATTTAAAT 1 cut(s) 751
SmiMI CAYNNNNRTG 3 cut(s) 1356, 1430, 2036
SmlI CTYRAG 2 cut(s) 1195, 1691
SmoI CTYRAG 2 cut(s) 1195, 1691
SpeI ACTAGT 1 cut(s) 586
SseBI AGGCCT 1 cut(s) 621
SspI AATATT 1 cut(s) 80
SspMI CTAG 8 cut(s) 96, 477, 587, 842, 1037, 1101, 1304, 1877
StuI AGGCCT 1 cut(s) 621
StyD4I CCNGG 4 cut(s) 212, 1534, 1622, 1789
StyI CCWWGG 1 cut(s) 476
SwaI ATTTAAAT 1 cut(s) 751
TaaI ACNGT 7 cut(s) 133, 265, 1662, 1741, 1762, 1786, 1929
TaiI ACGT 5 cut(s) 1413, 1465, 1895, 1925, 2142
TaqI TCGA 7 cut(s) 347, 1459, 1467, 1736, 1913, 1938, 2051
TaqII GACCGA 1 cut(s) 691
TatI WGTACW 5 cut(s) 893, 1084, 1662, 2163, 2170
TauI GCSGC 4 cut(s) 1224, 1775, 1778, 1815
TfiI GAWTC 4 cut(s) 1022, 1436, 1594, 1915
Tru1I TTAA 9 cut(s) 165, 317, 591, 750, 768, 948, 1196, 1326, 2239
Tru9I TTAA 9 cut(s) 165, 317, 591, 750, 768, 948, 1196, 1326, 2239
TscAI CASTG 2 cut(s) 138, 2040
TseFI GTSAC 7 cut(s) 56, 845, 1111, 1893, 1923, 2008, 2104
Tsp45I GTSAC 7 cut(s) 56, 845, 1111, 1893, 1923, 2008, 2104
TspGWI ACGGA 2 cut(s) 1311, 2184
TspRI CASTG 2 cut(s) 138, 2040
Vha464I CTTAAG 1 cut(s) 1195
VneI GTGCAC 1 cut(s) 134
VpaK11BI GGWCC 3 cut(s) 674, 1538, 1719
VspI ATTAAT 1 cut(s) 768
XagI CCTNNNNNAGG 1 cut(s) 305
XapI RAATTY 1 cut(s) 966
XceI RCATGY 3 cut(s) 539, 894, 1385
XcmI CCANNNNNNNNNTGG 1 cut(s) 1450
XmaJI CCTAGG 1 cut(s) 476
XmiI GTMKAC 3 cut(s) 429, 578, 1332
XspI CTAG 8 cut(s) 96, 477, 587, 842, 1037, 1101, 1304, 1877
ZraI GACGTC 2 cut(s) 1411, 1923
ZrmI AGTACT 1 cut(s) 1664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.