FvH4_3g07350

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012730
Physical Location & Seq
Forward (+)
4354643 .. 4358849
4207 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g07350.t1

Sequence Viewer

Length: 648 bp
ATGGCAGGGTACAGAGCTGAGGACGACTACGACTACCTGTTCAAAGTGGTCCTGATCGGCGACTCCGGCGTCGGCAAGTCCAACTTGCTCTCCCGATTCACCCGCAACGAGTTCAGCCTCGAGTCCAAGTCCACCATTGGCGTCGAGTTCGCCACTCGCAGCTTGAACGTCGACTCCAAAGTCATCAAGGCCCAGATTTGGGACACTGCCGGCCAAGAAAGGTATAGAGCAATAACTAGTGCCTATTATCGAGGGGCTGTAGGCGCACTGCTTGTATATGATGTTACTCGGAATTCAACATTTCAAAGTGTGGAAAGATGGTTAAAGGAATTAAGAGATCATACTGATCCCAACATTGTTGTCATGCTGGTTGGTAACAAATCAGACTTACGTCACCTTGTGGCAGTCTCAACTGAGGATGGAAAATCCTTTGCCGAAAGAGAGTCCCTCTACTTCATGGAAACTTCTGCACTCGAAGCAACTAATGTGGACAATGCATTTGCTGAAGTCCTTACCCAGATCTTTCACATAGTGAGCAAGAAGTCCATGGAGGCTAACAATGATGCAGCTGCTGTTCCATCCCAAGGAGAAAAAATCGATGTCAGTGATGTTTCAGCCGTAAAGAAAGGTGGGTGTTGTTCGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

216

Amino Acids

23.73

Weight (kDa)

5.32

Isoelectric Point (pI)

27.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 14 - 169 1.7e-12 ADP-ribosylation factor family
Ras PF00071 15 - 174 2e-62 Ras family
Roc PF08477 15 - 129 3.7e-35 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 15 - 121 7.2e-07 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012730)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18800 AT5G45750
fragaria_vesca FvH4_3g07350
malus_domestica MD00G1009500.v1.1 MD05G1295600.v1.1 MD10G1275700.v1.1
prunus_persica Prupe.4G066500_v2.0.a1
pyrus_communis pycom05g27460
rosa_chinensis RchiOBHm_Chr5g0011591
rosa_laevigata RLG00000031845
rosa_multiflora Rmu_sc0009001.1_g000012
rosa_roxburghii Rroxscaffold_1G00064900
rosa_rugosa Rorug04G0457900
rosa_samantha Rh5AG089600 Rh5BG084900 Rh5CG099000 Rh5DG084900
rosa_wichuraiana Rw5G007790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 68, 179
AccI GTMKAC 1 cut(s) 171
AciI CCGC 1 cut(s) 103
AclWI GGATC 1 cut(s) 341
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 525
AcyI GRCGYC 2 cut(s) 69, 141
AdeI CACNNNGTG 2 cut(s) 400, 532
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 3 cut(s) 198, 199, 584
AgsI TTSAA 4 cut(s) 43, 166, 297, 305
AhlI ACTAGT 1 cut(s) 236
AloI GAACNNNNNNTCC 2 cut(s) 158, 190
AluBI AGCT 4 cut(s) 17, 162, 569, 645
AluI AGCT 4 cut(s) 17, 162, 569, 645
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 1 cut(s) 341
AlwNI CAGNNNCTG 1 cut(s) 572
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 2 cut(s) 189, 211
ApeKI GCWGC 3 cut(s) 159, 566, 569
ApoI RAATTY 1 cut(s) 292
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
AspLEI GCGC 1 cut(s) 266
AspS9I GGNCC 2 cut(s) 49, 190
AsuHPI GGTGA 2 cut(s) 91, 386
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 1 cut(s) 49
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 3 cut(s) 171, 556, 578
BccI CCATC 3 cut(s) 312, 413, 586
BceAI ACGGC 1 cut(s) 602
BcoDI GTCTC 1 cut(s) 412
BcuI ACTAGT 1 cut(s) 236
BfaI CTAG 2 cut(s) 237, 646
BfmI CTRYAG 1 cut(s) 258
BglII AGATCT 1 cut(s) 519
BisI GCNGC 3 cut(s) 160, 567, 570
BlsI GCNGC 3 cut(s) 161, 568, 571
Bme18I GGWCC 1 cut(s) 49
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 2 cut(s) 49, 190
BmsI GCATC 1 cut(s) 553
BoxI GACNNNNGTC 1 cut(s) 390
BplI GAGNNNNNCTC 2 cut(s) 432, 464
Bpu10I CCTNAGC 1 cut(s) 18
Bsa29I ATCGAT 1 cut(s) 597
BsaHI GRCGYC 2 cut(s) 69, 141
BsaJI CCNNGG 2 cut(s) 546, 583
BsaXI ACNNNNNCTCC 4 cut(s) 74, 104, 158, 188
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 199, 584
Bse118I RCCGGY 1 cut(s) 209
BseCI ATCGAT 1 cut(s) 597
BseDI CCNNGG 2 cut(s) 546, 583
BseGI GGATG 2 cut(s) 424, 578
BseLI CCNNNNNNNGG 3 cut(s) 198, 199, 584
BseMII CTCAG 2 cut(s) 9, 405
BseXI GCAGC 3 cut(s) 171, 556, 578
BsgI GTGCAG 1 cut(s) 453
BshFI GGCC 2 cut(s) 191, 213
BshVI ATCGAT 1 cut(s) 597
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 2 cut(s) 66, 210
BslFI GGGAC 2 cut(s) 215, 430
BslI CCNNNNNNNGG 3 cut(s) 198, 199, 584
BsmAI GTCTC 1 cut(s) 412
BsmFI GGGAC 2 cut(s) 215, 430
BsnI GGCC 2 cut(s) 191, 213
BsoBI CYCGRG 1 cut(s) 119
Bsp143I GATC 4 cut(s) 54, 337, 346, 519
Bsp19I CCATGG 1 cut(s) 546
BspACI CCGC 1 cut(s) 103
BspANI GGCC 2 cut(s) 191, 213
BspCNI CTCAG 2 cut(s) 10, 406
BspDI ATCGAT 1 cut(s) 597
BspPI GGATC 1 cut(s) 341
BsrFI RCCGGY 1 cut(s) 209
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 2 cut(s) 546, 583
BssMI GATC 4 cut(s) 54, 337, 346, 519
BssNI GRCGYC 2 cut(s) 69, 141
BssT1I CCWWGG 2 cut(s) 546, 583
BstACI GRCGYC 2 cut(s) 69, 141
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 2 cut(s) 18, 414
BstDSI CCRYGG 1 cut(s) 546
BstF5I GGATG 2 cut(s) 424, 578
BstHHI GCGC 1 cut(s) 266
BstKTI GATC 4 cut(s) 57, 340, 349, 522
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 4 cut(s) 54, 337, 346, 519
BstMWI GCNNNNNNNGC 3 cut(s) 66, 263, 476
BstPAI GACNNNNGTC 1 cut(s) 390
BstSFI CTRYAG 1 cut(s) 258
BstV1I GCAGC 3 cut(s) 171, 556, 578
BstX2I RGATCY 1 cut(s) 519
BstYI RGATCY 1 cut(s) 519
Bsu15I ATCGAT 1 cut(s) 597
BsuRI GGCC 2 cut(s) 191, 213
BsuTUI ATCGAT 1 cut(s) 597
BtgI CCRYGG 1 cut(s) 546
BtsCI GGATG 2 cut(s) 424, 578
BtsI GCAGTG 2 cut(s) 204, 266
BtsIMutI CAGTG 3 cut(s) 204, 266, 610
Cac8I GCNNGC 1 cut(s) 211
CaiI CAGNNNCTG 1 cut(s) 572
CfoI GCGC 1 cut(s) 266
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 2 cut(s) 49, 190
ClaI ATCGAT 1 cut(s) 597
CseI GACGC 2 cut(s) 58, 130
Csp6I GTAC 1 cut(s) 10
CspCI CAANNNNNGTGG 2 cut(s) 468, 503
CviAII CATG 3 cut(s) 364, 457, 547
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 2 cut(s) 18, 414
DpnI GATC 4 cut(s) 56, 339, 348, 521
DpnII GATC 4 cut(s) 54, 337, 346, 519
DraIII CACNNNGTG 2 cut(s) 400, 532
DrdI GACNNNNNNGTC 2 cut(s) 68, 179
DseDI GACNNNNNNGTC 2 cut(s) 68, 179
EaeI YGGCCR 1 cut(s) 211
Eco130I CCWWGG 2 cut(s) 546, 583
Eco47I GGWCC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 292
EcoT14I CCWWGG 2 cut(s) 546, 583
EcoT22I ATGCAT 1 cut(s) 499
ErhI CCWWGG 2 cut(s) 546, 583
FaeI CATG 3 cut(s) 367, 460, 550
FaiI YATR 8 cut(s) 225, 277, 279, 342, 365, 458, 530, 548
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 2 cut(s) 215, 430
FatI CATG 3 cut(s) 363, 456, 546
FauI CCCGC 1 cut(s) 110
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 3 cut(s) 160, 567, 570
FokI GGATG 2 cut(s) 431, 565
Fsp4HI GCNGC 3 cut(s) 160, 567, 570
FspBI CTAG 2 cut(s) 237, 646
GlaI GCGC 1 cut(s) 265
GluI GCNGC 3 cut(s) 160, 567, 570
HaeIII GGCC 2 cut(s) 191, 213
HapII CCGG 2 cut(s) 66, 210
HgaI GACGC 2 cut(s) 58, 130
HhaI GCGC 1 cut(s) 266
Hin1I GRCGYC 2 cut(s) 69, 141
Hin1II CATG 3 cut(s) 367, 460, 550
Hin6I GCGC 1 cut(s) 264
HinP1I GCGC 1 cut(s) 264
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 5 cut(s) 62, 96, 122, 173, 443
HpaII CCGG 2 cut(s) 66, 210
HphI GGTGA 2 cut(s) 91, 386
Hpy166II GTNNAC 3 cut(s) 132, 172, 490
Hpy188I TCNGA 2 cut(s) 291, 385
Hpy188III TCNNGA 2 cut(s) 52, 93
Hpy8I GTNNAC 3 cut(s) 132, 172, 490
Hpy99I CGWCG 3 cut(s) 74, 146, 173
HpyCH4IV ACGT 2 cut(s) 168, 391
HpyCH4V TGCA 3 cut(s) 470, 497, 566
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 263, 476
HpyF3I CTNAG 2 cut(s) 18, 414
HpySE526I ACGT 2 cut(s) 168, 391
Hsp92I GRCGYC 2 cut(s) 69, 141
Hsp92II CATG 3 cut(s) 367, 460, 550
HspAI GCGC 1 cut(s) 264
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Kzo9I GATC 4 cut(s) 54, 337, 346, 519
LpnPI CCDG 7 cut(s) 50, 65, 79, 206, 223, 353, 530
Lsp1109I GCAGC 3 cut(s) 171, 556, 578
LweI GCATC 1 cut(s) 553
MaeI CTAG 2 cut(s) 237, 646
MaeII ACGT 2 cut(s) 168, 391
MaeIII GTNAC 3 cut(s) 283, 374, 392
MalI GATC 4 cut(s) 56, 339, 348, 521
MboI GATC 4 cut(s) 54, 337, 346, 519
MflI RGATCY 1 cut(s) 519
MluCI AATT 2 cut(s) 292, 329
MlyI GAGTC 4 cut(s) 56, 131, 167, 452
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 6 cut(s) 13, 128, 245, 409, 458, 544
Mph1103I ATGCAT 1 cut(s) 499
MroNI GCCGGC 1 cut(s) 209
MseI TTAA 2 cut(s) 323, 332
MspA1I CMGCKG 1 cut(s) 569
MspI CCGG 2 cut(s) 66, 210
MwoI GCNNNNNNNGC 3 cut(s) 66, 263, 476
NaeI GCCGGC 1 cut(s) 211
NcoI CCATGG 1 cut(s) 546
NdeII GATC 4 cut(s) 54, 337, 346, 519
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 3 cut(s) 367, 460, 550
NmuCI GTSAC 1 cut(s) 392
NsiI ATGCAT 1 cut(s) 499
PaeR7I CTCGAG 1 cut(s) 119
PdiI GCCGGC 1 cut(s) 211
PfeI GAWTC 1 cut(s) 96
PkrI GCNGC 3 cut(s) 161, 568, 571
PleI GAGTC 4 cut(s) 56, 130, 167, 451
PpsI GAGTC 4 cut(s) 56, 130, 167, 451
PshAI GACNNNNGTC 1 cut(s) 390
PspPI GGNCC 2 cut(s) 49, 190
PspXI VCTCGAGB 1 cut(s) 119
PstNI CAGNNNCTG 1 cut(s) 572
PsuI RGATCY 1 cut(s) 519
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 323, 332
SatI GCNGC 3 cut(s) 160, 567, 570
Sau3AI GATC 4 cut(s) 54, 337, 346, 519
Sau96I GGNCC 2 cut(s) 49, 190
SchI GAGTC 4 cut(s) 56, 131, 167, 452
SfaNI GCATC 1 cut(s) 553
SfcI CTRYAG 1 cut(s) 258
Sfr274I CTCGAG 1 cut(s) 119
SinI GGWCC 1 cut(s) 49
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
SpeI ACTAGT 1 cut(s) 236
Sse9I AATT 2 cut(s) 292, 329
SsiI CCGC 1 cut(s) 103
SspMI CTAG 2 cut(s) 237, 646
StyI CCWWGG 2 cut(s) 546, 583
TaiI ACGT 2 cut(s) 171, 394
TaqI TCGA 7 cut(s) 120, 144, 171, 250, 474, 597, 641
TasI AATT 2 cut(s) 292, 329
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 2 cut(s) 323, 332
Tru9I TTAA 2 cut(s) 323, 332
TscAI CASTG 3 cut(s) 211, 273, 610
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 3 cut(s) 159, 566, 569
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 1 cut(s) 445
TspRI CASTG 3 cut(s) 211, 273, 610
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 1 cut(s) 292
XhoI CTCGAG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 2 cut(s) 237, 646
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.