MD05G1295600.v1.1

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012730
Physical Location & Seq
Forward (+)
43015669 .. 43019350
3682 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1295600.v1.1.491

Sequence Viewer

Length: 651 bp
ATGGCTGGGTACAGAGCTGAGGACGACTACGACTACCTCTTCAAGTTGGTGCTGATCGGCGACTCCGGCGTCGGCAAGTCCAACTTGCTGTCGAGGTTCACCAGAAACGAGTTCAGCCTCGAGTCCAAGTCCACAATCGGAGTCGAGTTCGCCACCCGCAGCTTGGATGTCGACGGCAAAGTCATCAAGGCCCAGATTTGGGACACTGCTGGCCAAGAAAGGTACCGAGCGATAACAAGTGCTTACTATCGAGGGGCTGTTGGTGCACTACTTGTGTACGATGTCACTCGGAATTCAACATTTGAAAGTGTGGAAAGGTGGTTAAAGGAGTTAAGAGACCATACTGATCCCAACATTGTTGTCATGCTTGTTGGTAACAAATCAGATCTTCGTCACCTTGTGGCGGTCTCAACTGAGGACGGAAAATCCTTTGCCGAGAGGGAGTCTCTCTACTTCATGGAAACCTCTGCACTCGAAGCGACCAACGTGGACAATGCATTTTCTGAAGTCCTCACCCAGATCTTTCGCATTGTGAGCAGGAAGGCCATGGAGGCTGGCGATGGAGCTGCTTCGACCGTTCCTCAAGGAGAGAAAATCGATGTCAGTAAAGATGTTTCAGCTGTGAAGAAAGCTGGATGTTGCTCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

217

Amino Acids

23.82

Weight (kDa)

5.23

Isoelectric Point (pI)

22.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 14 - 170 3.3e-12 ADP-ribosylation factor family
Ras PF00071 15 - 174 1.5e-62 Ras family
Roc PF08477 15 - 129 1.1e-34 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 16 - 121 4.4e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012730)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18800 AT5G45750
fragaria_vesca FvH4_3g07350
malus_domestica MD00G1009500.v1.1 MD05G1295600.v1.1 MD10G1275700.v1.1
prunus_persica Prupe.4G066500_v2.0.a1
pyrus_communis pycom05g27460
rosa_chinensis RchiOBHm_Chr5g0011591
rosa_laevigata RLG00000031845
rosa_multiflora Rmu_sc0009001.1_g000012
rosa_roxburghii Rroxscaffold_1G00064900
rosa_rugosa Rorug04G0457900
rosa_samantha Rh5AG089600 Rh5BG084900 Rh5CG099000 Rh5DG084900
rosa_wichuraiana Rw5G007790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 68, 179
Acc65I GGTACC 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 222
AccI GTMKAC 1 cut(s) 171
AciI CCGC 2 cut(s) 157, 404
AclWI GGATC 1 cut(s) 341
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 525
AcyI GRCGYC 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 400
AfaI GTAC 3 cut(s) 11, 224, 278
AfiI CCNNNNNNNGG 4 cut(s) 163, 198, 199, 403
AgsI TTSAA 3 cut(s) 43, 297, 305
AluBI AGCT 6 cut(s) 17, 162, 566, 620, 632, 648
AluI AGCT 6 cut(s) 17, 162, 566, 620, 632, 648
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 3 cut(s) 330, 412, 450
Alw44I GTGCAC 1 cut(s) 264
AlwI GGATC 1 cut(s) 341
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 3 cut(s) 189, 211, 543
ApaLI GTGCAC 1 cut(s) 264
ApeKI GCWGC 2 cut(s) 159, 566
ApoI RAATTY 1 cut(s) 292
Asp718I GGTACC 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 190
AsuHPI GGTGA 3 cut(s) 91, 386, 505
AvaI CYCGRG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 268
BalI TGGCCA 1 cut(s) 213
BanI GGYRCC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 268
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 2 cut(s) 171, 553
BccI CCATC 1 cut(s) 554
BceAI ACGGC 1 cut(s) 190
BcgI CGANNNNNNTGC 2 cut(s) 548, 582
BcoDI GTCTC 3 cut(s) 330, 412, 450
BfaI CTAG 1 cut(s) 649
BglI GCCNNNNNGGC 1 cut(s) 551
BglII AGATCT 2 cut(s) 385, 519
BisI GCNGC 2 cut(s) 160, 567
BlsI GCNGC 2 cut(s) 161, 568
BmeT110I CYCGRG 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 224
BplI GAGNNNNNCTC 2 cut(s) 430, 462
Bpu10I CCTNAGC 1 cut(s) 18
BpuEI CTTGAG 2 cut(s) 567, 628
Bsa29I ATCGAT 1 cut(s) 597
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 2 cut(s) 330, 412
BsaJI CCNNGG 1 cut(s) 546
Bsc4I CCNNNNNNNGG 4 cut(s) 163, 198, 199, 403
BseCI ATCGAT 1 cut(s) 597
BseDI CCNNGG 1 cut(s) 546
BseGI GGATG 2 cut(s) 172, 641
BseLI CCNNNNNNNGG 4 cut(s) 163, 198, 199, 403
BseMII CTCAG 2 cut(s) 9, 405
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 2 cut(s) 171, 553
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 1 cut(s) 453
Bsh1285I CGRYCG 1 cut(s) 576
BshFI GGCC 3 cut(s) 191, 213, 545
BshNI GGYRCC 1 cut(s) 222
BshVI ATCGAT 1 cut(s) 597
BsiEI CGRYCG 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 268
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 1 cut(s) 66
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 4 cut(s) 163, 198, 199, 403
BsmAI GTCTC 3 cut(s) 330, 412, 450
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 3 cut(s) 191, 213, 545
Bso31I GGTCTC 2 cut(s) 330, 412
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 4 cut(s) 54, 346, 385, 519
Bsp19I CCATGG 1 cut(s) 546
BspACI CCGC 2 cut(s) 157, 404
BspANI GGCC 3 cut(s) 191, 213, 545
BspCNI CTCAG 2 cut(s) 10, 406
BspDI ATCGAT 1 cut(s) 597
BspLI GGNNCC 1 cut(s) 224
BspPI GGATC 1 cut(s) 341
BspT107I GGYRCC 1 cut(s) 222
BspTNI GGTCTC 2 cut(s) 330, 412
BssECI CCNNGG 1 cut(s) 546
BssMI GATC 4 cut(s) 54, 346, 385, 519
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 1 cut(s) 546
Bst4CI ACNGT 1 cut(s) 577
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 1 cut(s) 69
BstC8I GCNNGC 2 cut(s) 211, 556
BstDEI CTNAG 2 cut(s) 18, 414
BstDSI CCRYGG 1 cut(s) 546
BstF5I GGATG 2 cut(s) 172, 641
BstKTI GATC 4 cut(s) 57, 349, 388, 522
BstMAI GTCTC 3 cut(s) 330, 412, 450
BstMBI GATC 4 cut(s) 54, 346, 385, 519
BstMCI CGRYCG 1 cut(s) 576
BstMWI GCNNNNNNNGC 5 cut(s) 66, 263, 476, 534, 551
BstSLI GKGCMC 1 cut(s) 268
BstV1I GCAGC 2 cut(s) 171, 553
BstX2I RGATCY 2 cut(s) 385, 519
BstYI RGATCY 2 cut(s) 385, 519
Bsu15I ATCGAT 1 cut(s) 597
BsuRI GGCC 3 cut(s) 191, 213, 545
BsuTUI ATCGAT 1 cut(s) 597
BtgI CCRYGG 1 cut(s) 546
BtgZI GCGATG 1 cut(s) 573
BtsCI GGATG 2 cut(s) 172, 641
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 1 cut(s) 204
Cac8I GCNNGC 2 cut(s) 211, 556
Cfr13I GGNCC 1 cut(s) 190
ClaI ATCGAT 1 cut(s) 597
CseI GACGC 1 cut(s) 58
Csp6I GTAC 3 cut(s) 10, 223, 277
CviAII CATG 3 cut(s) 364, 457, 547
CviQI GTAC 3 cut(s) 10, 223, 277
DdeI CTNAG 2 cut(s) 18, 414
DpnI GATC 4 cut(s) 56, 348, 387, 521
DpnII GATC 4 cut(s) 54, 346, 385, 519
DraIII CACNNNGTG 1 cut(s) 400
DrdI GACNNNNNNGTC 2 cut(s) 68, 179
DseDI GACNNNNNNGTC 2 cut(s) 68, 179
EaeI YGGCCR 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 546
Eco31I GGTCTC 2 cut(s) 330, 412
Eco57I CTGAAG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 546
EcoT22I ATGCAT 1 cut(s) 499
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 3 cut(s) 367, 460, 550
FaiI YATR 4 cut(s) 342, 365, 458, 548
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 1 cut(s) 215
FatI CATG 3 cut(s) 363, 456, 546
FauI CCCGC 1 cut(s) 164
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 160, 567
FokI GGATG 1 cut(s) 179
Fsp4HI GCNGC 2 cut(s) 160, 567
FspBI CTAG 1 cut(s) 649
GluI GCNGC 2 cut(s) 160, 567
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 3 cut(s) 191, 213, 545
HapII CCGG 1 cut(s) 66
HgaI GACGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 3 cut(s) 367, 460, 550
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 4 cut(s) 62, 122, 141, 443
HpaII CCGG 1 cut(s) 66
HphI GGTGA 3 cut(s) 91, 386, 505
Hpy166II GTNNAC 6 cut(s) 99, 132, 172, 266, 277, 490
Hpy188I TCNGA 4 cut(s) 140, 291, 385, 505
Hpy8I GTNNAC 6 cut(s) 99, 132, 172, 266, 277, 490
Hpy99I CGWCG 2 cut(s) 74, 176
HpyAV CCTTC 1 cut(s) 535
HpyCH4III ACNGT 1 cut(s) 577
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 3 cut(s) 266, 470, 497
HpyF10VI GCNNNNNNNGC 5 cut(s) 66, 263, 476, 534, 551
HpyF3I CTNAG 2 cut(s) 18, 414
HpySE526I ACGT 1 cut(s) 486
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 3 cut(s) 367, 460, 550
KpnI GGTACC 1 cut(s) 226
Kzo9I GATC 4 cut(s) 54, 346, 385, 519
LmnI GCTCC 1 cut(s) 563
LpnPI CCDG 8 cut(s) 79, 115, 195, 206, 523, 530, 540, 618
Lsp1109I GCAGC 2 cut(s) 171, 553
MaeI CTAG 1 cut(s) 649
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 3 cut(s) 283, 374, 392
MalI GATC 4 cut(s) 56, 348, 387, 521
MboI GATC 4 cut(s) 54, 346, 385, 519
MboII GAAGA 3 cut(s) 31, 380, 637
MflI RGATCY 2 cut(s) 385, 519
MhlI GDGCHC 1 cut(s) 268
MlsI TGGCCA 1 cut(s) 213
MluCI AATT 1 cut(s) 292
MluNI TGGCCA 1 cut(s) 213
MlyI GAGTC 4 cut(s) 56, 131, 150, 452
MmeI TCCRAC 1 cut(s) 105
Mox20I TGGCCA 1 cut(s) 213
Mph1103I ATGCAT 1 cut(s) 499
MscI TGGCCA 1 cut(s) 213
MseI TTAA 2 cut(s) 323, 332
Msp20I TGGCCA 1 cut(s) 213
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 1 cut(s) 66
MwoI GCNNNNNNNGC 5 cut(s) 66, 263, 476, 534, 551
NcoI CCATGG 1 cut(s) 546
NdeII GATC 4 cut(s) 54, 346, 385, 519
NlaIII CATG 3 cut(s) 367, 460, 550
NlaIV GGNNCC 1 cut(s) 224
NmeAIII GCCGAG 1 cut(s) 460
NmuCI GTSAC 2 cut(s) 283, 392
NsiI ATGCAT 1 cut(s) 499
PaeR7I CTCGAG 1 cut(s) 119
PkrI GCNGC 2 cut(s) 161, 568
PleI GAGTC 4 cut(s) 56, 130, 149, 451
PpsI GAGTC 4 cut(s) 56, 130, 149, 451
PspFI CCCAGC 1 cut(s) 5
PspN4I GGNNCC 1 cut(s) 224
PspPI GGNCC 1 cut(s) 190
PspXI VCTCGAGB 1 cut(s) 119
PsuI RGATCY 2 cut(s) 385, 519
PvuII CAGCTG 1 cut(s) 620
RsaI GTAC 3 cut(s) 11, 224, 278
RsaNI GTAC 3 cut(s) 10, 223, 277
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 323, 332
SatI GCNGC 2 cut(s) 160, 567
Sau3AI GATC 4 cut(s) 54, 346, 385, 519
Sau96I GGNCC 1 cut(s) 190
SchI GAGTC 4 cut(s) 56, 131, 150, 452
SduI GDGCHC 1 cut(s) 268
Sfr274I CTCGAG 1 cut(s) 119
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 3 cut(s) 119, 582, 643
SmoI CTYRAG 3 cut(s) 119, 582, 643
Sse9I AATT 1 cut(s) 292
SsiI CCGC 2 cut(s) 157, 404
SspMI CTAG 1 cut(s) 649
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 1 cut(s) 577
TaiI ACGT 1 cut(s) 489
TaqI TCGA 8 cut(s) 92, 120, 144, 171, 250, 474, 572, 597
TasI AATT 1 cut(s) 292
Tru1I TTAA 2 cut(s) 323, 332
Tru9I TTAA 2 cut(s) 323, 332
TscAI CASTG 1 cut(s) 211
TseFI GTSAC 2 cut(s) 283, 392
TseI GCWGC 2 cut(s) 159, 566
Tsp45I GTSAC 2 cut(s) 283, 392
TspDTI ATGAA 1 cut(s) 445
TspGWI ACGGA 1 cut(s) 435
TspRI CASTG 1 cut(s) 211
VneI GTGCAC 1 cut(s) 264
XapI RAATTY 1 cut(s) 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XhoI CTCGAG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 649
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.