MD10G1275700.v1.1

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012730
Physical Location & Seq
Forward (+)
36689509 .. 36693254
3746 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1275700.v1.1.491

Sequence Viewer

Length: 654 bp
ATGGCTGGGTACAGAGCTGAGGACGACTACGACTACCTTTTCAAGGTGGTGCTGATCGGCGACTCCGGCGTTGGCAAGTCCAACTTGCTGTCGAGGTTCACCAGGAACGAGTTCAGCCTCGAGTCTAAGTCCACCATCGGCGTCGAGTTCGCCACCCGCAGCTTGGATGTCGACGGCAAAGTCATCAAGGCTCAGATTTGGGACACTGCCGGCCAAGAAAGGTACCGGGCAATAACAAGTGCTTACTATCGAGGGGCTGTGGGTGCACTGCTTGTGTATGATGTCACTCGGAATTCAACTTTTGAAAGTGTGGAAAGGTGGTTAAAGGAGTTAAGAGACCACACTGATCCCAACATTGTCGTCATGCTTGTTGGTAACAAATCAGATCTTCGTCATCTTGTGGCAGTCTCAACTGAGGACGGAAAATCCTTTGCCGAGAGGGAGTCTCTCTACTTCATGGAAACTTCTGCACTCGAAGCGACCAACGTGGATAACGCATTTTCTGAAGTCCTCACTCAGATCTTTCGAGTCGTGAGCAGGAAAGCCATGGAGTCTGGCAATGAAGGAGCTGCTTCAGCTGTTCCTCAAGGGGAGAAAATCGATGTTGGTAAAGATGTTTCAGCTGTGAAGAAAGGTGGGTGTTGCTCAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

218

Amino Acids

23.88

Weight (kDa)

5.24

Isoelectric Point (pI)

22.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 14 - 170 2.4e-12 ADP-ribosylation factor family
Ras PF00071 15 - 174 1.6e-62 Ras family
Roc PF08477 15 - 129 6e-35 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 15 - 121 1.2e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012730)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18800 AT5G45750
fragaria_vesca FvH4_3g07350
malus_domestica MD00G1009500.v1.1 MD05G1295600.v1.1 MD10G1275700.v1.1
prunus_persica Prupe.4G066500_v2.0.a1
pyrus_communis pycom05g27460
rosa_chinensis RchiOBHm_Chr5g0011591
rosa_laevigata RLG00000031845
rosa_multiflora Rmu_sc0009001.1_g000012
rosa_roxburghii Rroxscaffold_1G00064900
rosa_rugosa Rorug04G0457900
rosa_samantha Rh5AG089600 Rh5BG084900 Rh5CG099000 Rh5DG084900
rosa_wichuraiana Rw5G007790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
Acc65I GGTACC 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 222
AccI GTMKAC 1 cut(s) 171
AciI CCGC 1 cut(s) 157
AclWI GGATC 1 cut(s) 341
AcoI YGGCCR 1 cut(s) 211
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 2 cut(s) 525, 558
AcyI GRCGYC 1 cut(s) 141
AfaI GTAC 2 cut(s) 11, 224
AfiI CCNNNNNNNGG 2 cut(s) 43, 163
AgsI TTSAA 3 cut(s) 43, 297, 305
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 5 cut(s) 17, 162, 569, 578, 623
AluI AGCT 5 cut(s) 17, 162, 569, 578, 623
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 3 cut(s) 330, 412, 450
Alw44I GTGCAC 1 cut(s) 264
AlwI GGATC 1 cut(s) 341
Ama87I CYCGRG 1 cut(s) 119
AoxI GGCC 1 cut(s) 211
ApaLI GTGCAC 1 cut(s) 264
ApeKI GCWGC 2 cut(s) 159, 569
ApoI RAATTY 1 cut(s) 292
Asp700I GAANNNNTTC 1 cut(s) 110
Asp718I GGTACC 1 cut(s) 222
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 268
BanI GGYRCC 1 cut(s) 222
Bbv12I GWGCWC 1 cut(s) 268
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 2 cut(s) 171, 556
BccI CCATC 1 cut(s) 143
BceAI ACGGC 1 cut(s) 190
BciT130I CCWGG 1 cut(s) 103
BcnI CCSGG 1 cut(s) 227
BcoDI GTCTC 3 cut(s) 330, 412, 450
BfaI CTAG 1 cut(s) 652
BglII AGATCT 2 cut(s) 385, 519
BisI GCNGC 2 cut(s) 160, 570
BlsI GCNGC 2 cut(s) 161, 571
Bme1390I CCNGG 2 cut(s) 103, 227
BmeT110I CYCGRG 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 224
BmrFI CCNGG 2 cut(s) 103, 227
BplI GAGNNNNNCTC 2 cut(s) 430, 462
Bpu10I CCTNAGC 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 570
BpuMI CCSGG 1 cut(s) 227
Bsa29I ATCGAT 1 cut(s) 600
BsaHI GRCGYC 1 cut(s) 141
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 1 cut(s) 546
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 163
Bse118I RCCGGY 1 cut(s) 209
Bse3DI GCAATG 1 cut(s) 565
BseBI CCWGG 1 cut(s) 103
BseCI ATCGAT 1 cut(s) 600
BseDI CCNNGG 1 cut(s) 546
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 2 cut(s) 43, 163
BseMI GCAATG 1 cut(s) 565
BseMII CTCAG 4 cut(s) 9, 206, 405, 530
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 2 cut(s) 171, 556
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 1 cut(s) 453
BshFI GGCC 1 cut(s) 213
BshNI GGYRCC 1 cut(s) 222
BshVI ATCGAT 1 cut(s) 600
BsiHKAI GWGCWC 1 cut(s) 268
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 3 cut(s) 66, 210, 226
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 2 cut(s) 43, 163
BsmAI GTCTC 3 cut(s) 330, 412, 450
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 1 cut(s) 213
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 4 cut(s) 54, 346, 385, 519
Bsp19I CCATGG 1 cut(s) 546
BspACI CCGC 1 cut(s) 157
BspANI GGCC 1 cut(s) 213
BspCNI CTCAG 4 cut(s) 10, 205, 406, 529
BspDI ATCGAT 1 cut(s) 600
BspLI GGNNCC 1 cut(s) 224
BspPI GGATC 1 cut(s) 341
BspT107I GGYRCC 1 cut(s) 222
BspTNI GGTCTC 1 cut(s) 330
BsrDI GCAATG 1 cut(s) 565
BsrFI RCCGGY 1 cut(s) 209
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 546
BssMI GATC 4 cut(s) 54, 346, 385, 519
BssNI GRCGYC 1 cut(s) 141
BssT1I CCWWGG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 103
BstACI GRCGYC 1 cut(s) 141
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 5 cut(s) 18, 126, 192, 414, 516
BstDSI CCRYGG 1 cut(s) 546
BstENI CCTNNNNNAGG 1 cut(s) 41
BstF5I GGATG 1 cut(s) 172
BstKTI GATC 4 cut(s) 57, 349, 388, 522
BstMAI GTCTC 3 cut(s) 330, 412, 450
BstMBI GATC 4 cut(s) 54, 346, 385, 519
BstMWI GCNNNNNNNGC 4 cut(s) 66, 263, 476, 575
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 2 cut(s) 101, 225
BstSLI GKGCMC 1 cut(s) 268
BstV1I GCAGC 2 cut(s) 171, 556
BstX2I RGATCY 2 cut(s) 385, 519
BstYI RGATCY 2 cut(s) 385, 519
Bsu15I ATCGAT 1 cut(s) 600
BsuRI GGCC 1 cut(s) 213
BsuTUI ATCGAT 1 cut(s) 600
BtgI CCRYGG 1 cut(s) 546
BtsCI GGATG 1 cut(s) 172
BtsI GCAGTG 2 cut(s) 204, 266
BtsIMutI CAGTG 3 cut(s) 204, 266, 342
Cac8I GCNNGC 1 cut(s) 211
Cfr10I RCCGGY 1 cut(s) 209
ClaI ATCGAT 1 cut(s) 600
CseI GACGC 1 cut(s) 130
Csp6I GTAC 2 cut(s) 10, 223
CviAII CATG 3 cut(s) 364, 457, 547
CviQI GTAC 2 cut(s) 10, 223
DdeI CTNAG 5 cut(s) 18, 126, 192, 414, 516
DpnI GATC 4 cut(s) 56, 348, 387, 521
DpnII GATC 4 cut(s) 54, 346, 385, 519
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
EaeI YGGCCR 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 546
Eco31I GGTCTC 1 cut(s) 330
Eco57I CTGAAG 2 cut(s) 525, 558
Eco88I CYCGRG 1 cut(s) 119
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 3 cut(s) 367, 460, 550
FaiI YATR 4 cut(s) 279, 365, 458, 548
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 1 cut(s) 215
FatI CATG 3 cut(s) 363, 456, 546
FauI CCCGC 1 cut(s) 164
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 160, 570
FokI GGATG 1 cut(s) 179
Fsp4HI GCNGC 2 cut(s) 160, 570
FspBI CTAG 1 cut(s) 652
GluI GCNGC 2 cut(s) 160, 570
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 1 cut(s) 213
HapII CCGG 3 cut(s) 66, 210, 226
HgaI GACGC 1 cut(s) 130
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 3 cut(s) 367, 460, 550
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 5 cut(s) 62, 122, 443, 528, 551
HpaII CCGG 3 cut(s) 66, 210, 226
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 4 cut(s) 99, 132, 172, 266
Hpy188I TCNGA 5 cut(s) 195, 291, 385, 505, 519
Hpy188III TCNNGA 1 cut(s) 532
Hpy8I GTNNAC 4 cut(s) 99, 132, 172, 266
Hpy99I CGWCG 2 cut(s) 146, 176
HpyAV CCTTC 1 cut(s) 557
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 2 cut(s) 266, 470
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 263, 476, 575
HpyF3I CTNAG 5 cut(s) 18, 126, 192, 414, 516
HpySE526I ACGT 1 cut(s) 486
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 3 cut(s) 367, 460, 550
KpnI GGTACC 1 cut(s) 226
KroI GCCGGC 1 cut(s) 209
KroNI GCCGGC 1 cut(s) 211
Kzo9I GATC 4 cut(s) 54, 346, 385, 519
LmnI GCTCC 1 cut(s) 566
LpnPI CCDG 7 cut(s) 79, 88, 115, 223, 239, 523, 540
Lsp1109I GCAGC 2 cut(s) 171, 556
MaeI CTAG 1 cut(s) 652
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 2 cut(s) 283, 374
MalI GATC 4 cut(s) 56, 348, 387, 521
MboI GATC 4 cut(s) 54, 346, 385, 519
MboII GAAGA 2 cut(s) 380, 640
MflI RGATCY 2 cut(s) 385, 519
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 1 cut(s) 292
MlyI GAGTC 5 cut(s) 56, 131, 452, 537, 560
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 8 cut(s) 13, 87, 128, 245, 409, 432, 521, 594
MroNI GCCGGC 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 110
MseI TTAA 2 cut(s) 323, 332
MspA1I CMGCKG 2 cut(s) 578, 623
MspI CCGG 3 cut(s) 66, 210, 226
MspR9I CCNGG 2 cut(s) 103, 227
MvaI CCWGG 1 cut(s) 103
MwoI GCNNNNNNNGC 4 cut(s) 66, 263, 476, 575
NaeI GCCGGC 1 cut(s) 211
NciI CCSGG 1 cut(s) 227
NcoI CCATGG 1 cut(s) 546
NdeII GATC 4 cut(s) 54, 346, 385, 519
NgoMIV GCCGGC 1 cut(s) 209
NlaIII CATG 3 cut(s) 367, 460, 550
NlaIV GGNNCC 1 cut(s) 224
NmeAIII GCCGAG 1 cut(s) 460
NmuCI GTSAC 1 cut(s) 283
PaeR7I CTCGAG 1 cut(s) 119
PdiI GCCGGC 1 cut(s) 211
PdmI GAANNNNTTC 1 cut(s) 110
PkrI GCNGC 2 cut(s) 161, 571
PleI GAGTC 5 cut(s) 56, 130, 451, 536, 559
PpsI GAGTC 5 cut(s) 56, 130, 451, 536, 559
Psp6I CCWGG 1 cut(s) 101
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 1 cut(s) 224
PspXI VCTCGAGB 1 cut(s) 119
PsuI RGATCY 2 cut(s) 385, 519
PvuII CAGCTG 2 cut(s) 578, 623
RsaI GTAC 2 cut(s) 11, 224
RsaNI GTAC 2 cut(s) 10, 223
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 2 cut(s) 323, 332
SatI GCNGC 2 cut(s) 160, 570
Sau3AI GATC 4 cut(s) 54, 346, 385, 519
SchI GAGTC 5 cut(s) 56, 131, 452, 537, 560
ScrFI CCNGG 2 cut(s) 103, 227
SduI GDGCHC 1 cut(s) 268
Sfr274I CTCGAG 1 cut(s) 119
SlaI CTCGAG 1 cut(s) 119
SmlI CTYRAG 2 cut(s) 119, 585
SmoI CTYRAG 2 cut(s) 119, 585
Sse9I AATT 1 cut(s) 292
SsiI CCGC 1 cut(s) 157
SspMI CTAG 1 cut(s) 652
StyD4I CCNGG 2 cut(s) 101, 225
StyI CCWWGG 1 cut(s) 546
TaiI ACGT 1 cut(s) 489
TaqI TCGA 8 cut(s) 92, 120, 144, 171, 250, 474, 526, 600
TasI AATT 1 cut(s) 292
Tru1I TTAA 2 cut(s) 323, 332
Tru9I TTAA 2 cut(s) 323, 332
TscAI CASTG 3 cut(s) 211, 273, 349
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 2 cut(s) 159, 569
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 2 cut(s) 445, 576
TspGWI ACGGA 1 cut(s) 435
TspRI CASTG 3 cut(s) 211, 273, 349
VneI GTGCAC 1 cut(s) 264
XagI CCTNNNNNAGG 1 cut(s) 41
XapI RAATTY 1 cut(s) 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XhoI CTCGAG 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 110
XspI CTAG 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.