Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0010968

Physical Location & Seq

Genomic Coordinates Reverse (-)

5098826 .. 5102867

Gene Structure 4042 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g08740.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGGCTCTCAGCGCGTACCAGCTCGACCCGCAATCGGCGGCCCAGAAAGCCGTCGCGGCAATCGGGTCGGGTTATGACCTCTGCTACGATCTCCGGCTGAATTCGTGTAAGCCCATACTGATTGAACTGGATCCGACCCGGACCCGGGACCTCGTCGTGCCCGGCGGCGCCGTCGTGCCTAACGTCTCCACCTCCATCAAGTGCGACAAGGGCGAGCGCACCAAGTTTCGCTCCGACGTCGTTTCGTTCAATCAGATGTCAGAGCAGTACAATAAGGAACTTTCCTTAACGGGGAAAATTCCGTCCGGCTTGTTTAATGCTATGTTTAACTACACCGGCTCCTGGCAGAAGGATGCAGCTCCCACGAAATGTCTTGCTTTTGATGGTTGGTTAATAAATCTGTATAACATTGAGTTAGCGAGATCACAGTTAACACTATCGGAGCAAGTGAAACAAGACGTGCCTTCTCTATGGGACCCTGCTGCCCTTGCCGCATTCATTGATAAGTATGGTACTCACGTTGTTGTTGGGGTGAAGATGGGTGGAAAGGATATAGTTCACATAAAGCAGTTACAAAACTCAAAACTTGAGCCTGCTGAGCTGCAGAAATTATTAAAGCAATTAGCCGAGGAGAGATTTTCTGCTGATGTAACTGAATCCTCTATATCAAATACTGCTGATTTATTAGGAAAAATGAGGGATGACCACCCTATTCACTGGGATATTAAAAAAACATTAGCATCCTCAATAAGGCCACCCATCATCACTCATTCAAGGAATGATGATATAGTTACTATTAGTGTTCGAAGGGGAGGTGTTGATGTTGGTCAAAGTCATAACCAATGGCTTTCGACTATATCACAGGCGCCTAATGTCATATCAATGTCCTTTGTGCCCATAACATCACTTTTGGGTGGTATTCAAGGCAATGGATTCCTTAGCCATGCAGTAAATCTGTACCTTAGATATAAACCACCAATAGAGGAGCTTCAACAATTCTTAGAATTTCAATTACCACGGCAATGGGCTCCAGTATACGCTGATCTGCCTCTTGGTCTTAGGCGGAGAAGACATGCATCCCCAGCACTTCAATTCAGTTTTATGGGCCCCAAGCTATATGTTAACACTATGAAGGTTGATTCCGTAAATCGACCGGTAACAGGAATCCGTTTGTACCTTGAAGGTAAAAAAGGTGACCATTTGGCAATCCATCTTCAGCATCTTACAAATCTTCCCAGTTCATTCCAAGTCTCCACTGATCACAATTATGAGCCCATTGACGAGCCAGAGGAACGACGTTACTTCGAAGCTGTCAAGTGGAGCATATTTTCACATGTATGCACTGCCCCAGTAGAATATAAAGGTGCCTGCATTAATGACTCTGCTTCTATTGTGACAAAGGCCTGGTTTGAAGTCAAGGCTATGGGAATAAAGAAAGTTTTGTTCCTGAGGCTTGAATTTTCTACTGTGGCATCTGCTAGGATTCGAAGATCAGAATGGGAAGGCTCAAGCTCATCTCGTAAGTCAGGATTCATCTCAACCTTGATCAGCACACGGTTTAGTACTGCATTGAATCCACCTGTGACACCGGCAAAAGTGAATTTGAATTCAGCAGTGTACCCTGGTGGCCCACCTTTACCAGCCAAGGCACCAAAAATGGCGACGTTTGTAGATACCAAGGAAATGTTGAGAGGTCCTGAGGTCACCCCAGGATATTGGATGGTTACTGGAGCCAAGCTTTGTGTAGAGGGTGGTCGAATCTCAGTCAAGGTGAAGTATTCATTGCTGACAATTATGTTGGAAGACTCGATGATGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008219 GO:0009626 GO:0009628 GO:0009651 GO:0009987 GO:0010337 GO:0010565 GO:0010817 GO:0012501 GO:0019222 GO:0031323 GO:0032350 GO:0033036 GO:0033037 GO:0033554 GO:0034050 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051716 GO:0052542 GO:0052545 GO:0062012 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MACPF

Protein Analysis

607

Amino Acids

67.29

Weight (kDa)

9.08

Isoelectric Point (pI)

49.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MACPF	PF01823	104 - 320	1.5e-35	MAC/Perforin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28380
fragaria_vesca	FvH4_3g08740
malus_domestica	MD05G1311300.v1.1 MD10G1290300.v1.1
prunus_persica	Prupe.4G051500_v2.0.a1
pyrus_communis	pycom05g28740 pycom10g24250 pycom10g24280
rosa_chinensis	RchiOBHm_Chr5g0009001
rosa_laevigata	RLG00000031624
rosa_multiflora	Rmu_sc0021361.1_g000001
rosa_roxburghii	Rroxscaffold_1G00067010 Rroxscaffold_1G00067650
rosa_rugosa	Rorug04G0440800 Rorug04G0440900
rosa_samantha	Rh5AG069800 Rh5BG066500 Rh5CG077800 Rh5DG065800
rosa_wichuraiana	Rw5G006510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	240
AccB1I	GGYRCC	4 cut(s)	167, 865, 1364, 1648
AccI	GTMKAC	1 cut(s)	1035
AccII	CGCG	2 cut(s)	14, 56
AciI	CCGC	6 cut(s)	29, 38, 56, 165, 492, 1063
AclWI	GGATC	2 cut(s)	125, 138
AcsI	RAATTY	6 cut(s)	100, 297, 1004, 1457, 1600, 1606
AcuI	CTGAAG	1 cut(s)	1199
AcyI	GRCGYC	3 cut(s)	168, 237, 866
AfaI	GTAC	7 cut(s)	17, 269, 514, 959, 1175, 1564, 1619
AfiI	CCNNNNNNNGG	7 cut(s)	34, 144, 145, 291, 342, 750, 1160
AflIII	ACRYGT	1 cut(s)	1333
AgeI	ACCGGT	1 cut(s)	1153
AjiI	CACGTC	1 cut(s)	460
AjnI	CCWGG	4 cut(s)	341, 1403, 1621, 1708
AluBI	AGCT	8 cut(s)	22, 359, 601, 988, 1114, 1310, 1512, 1738
AluI	AGCT	8 cut(s)	22, 359, 601, 988, 1114, 1310, 1512, 1738
Alw26I	GTCTC	2 cut(s)	190, 1255
AlwI	GGATC	2 cut(s)	125, 138
Ama87I	CYCGRG	1 cut(s)	144
AoxI	GGCC	5 cut(s)	39, 752, 1105, 1401, 1627
ApaI	GGGCCC	1 cut(s)	1109
ApeKI	GCWGC	3 cut(s)	356, 482, 601
ApoI	RAATTY	6 cut(s)	100, 297, 1004, 1457, 1600, 1606
AseI	ATTAAT	1 cut(s)	1374
AsiGI	ACCGGT	1 cut(s)	1153
AspLEI	GCGC	4 cut(s)	14, 170, 219, 868
AspS9I	GGNCC	8 cut(s)	40, 141, 148, 475, 1105, 1106, 1628, 1694
AsuC2I	CCSGG	4 cut(s)	139, 145, 146, 162
AsuHPI	GGTGA	4 cut(s)	544, 1205, 1696, 1783
AsuII	TTCGAA	3 cut(s)	805, 1305, 1486
AvaI	CYCGRG	1 cut(s)	144
AvaII	GGWCC	4 cut(s)	141, 148, 475, 1694
AxyI	CCTNAGG	2 cut(s)	1448, 1698
BaeGI	GKGCMC	3 cut(s)	162, 897, 1109
BaeI	ACNNNNGTAYC	2 cut(s)	504, 537
BamHI	GGATCC	1 cut(s)	130
BanI	GGYRCC	4 cut(s)	167, 865, 1364, 1648
BanII	GRGCYC	3 cut(s)	1030, 1109, 1275
BbsI	GAAGAC	2 cut(s)	1075, 1809
BbvI	GCAGC	3 cut(s)	368, 469, 588
BccI	CCATC	6 cut(s)	203, 377, 532, 767, 1218, 1714
BceAI	ACGGC	3 cut(s)	35, 155, 1034
BcgI	CGANNNNNNTGC	2 cut(s)	48, 82
BciT130I	CCWGG	4 cut(s)	343, 1405, 1623, 1710
BclI	TGATCA	2 cut(s)	1258, 1545
BcnI	CCSGG	4 cut(s)	139, 145, 146, 162
BcoDI	GTCTC	2 cut(s)	190, 1255
BfaI	CTAG	1 cut(s)	1479
BfmI	CTRYAG	1 cut(s)	602
BfoI	RGCGCY	2 cut(s)	171, 869
BglI	GCCNNNNNGGC	1 cut(s)	56
BisI	GCNGC	7 cut(s)	39, 57, 166, 357, 483, 492, 602
BlpI	GCTNAGC	1 cut(s)	597
BlsI	GCNGC	7 cut(s)	40, 58, 167, 358, 484, 493, 603
BmcAI	AGTACT	1 cut(s)	1564
Bme1390I	CCNGG	8 cut(s)	139, 145, 146, 162, 343, 1405, 1623, 1710
Bme18I	GGWCC	4 cut(s)	141, 148, 475, 1694
BmeT110I	CYCGRG	1 cut(s)	144
BmgBI	CACGTC	1 cut(s)	460
BmgT120I	GGNCC	8 cut(s)	40, 141, 148, 475, 1105, 1106, 1628, 1694
BmrFI	CCNGG	8 cut(s)	139, 145, 146, 162, 343, 1405, 1623, 1710
BmrI	ACTGGG	3 cut(s)	727, 1230, 1343
BmsI	GCATC	5 cut(s)	343, 749, 1085, 1228, 1481
BmuI	ACTGGG	3 cut(s)	727, 1230, 1343
BpiI	GAAGAC	2 cut(s)	1075, 1809
BpmI	CTGGAG	2 cut(s)	1014, 1749
Bpu10I	CCTNAGC	1 cut(s)	938
Bpu1102I	GCTNAGC	1 cut(s)	597
Bpu14I	TTCGAA	3 cut(s)	805, 1305, 1486
BpuEI	CTTGAG	2 cut(s)	608, 1492
BpuMI	CCSGG	4 cut(s)	139, 145, 146, 162
BsaHI	GRCGYC	3 cut(s)	168, 237, 866
BsaJI	CCNNGG	7 cut(s)	144, 627, 1016, 1621, 1644, 1677, 1708
BsaWI	WCCGGW	1 cut(s)	1153
BsaXI	ACNNNNNCTCC	2 cut(s)	323, 353
Bsc4I	CCNNNNNNNGG	7 cut(s)	34, 144, 145, 291, 342, 750, 1160
Bse118I	RCCGGY	3 cut(s)	335, 1153, 1588
Bse1I	ACTGG	6 cut(s)	132, 722, 1031, 1236, 1349, 1732
Bse21I	CCTNAGG	2 cut(s)	1448, 1698
Bse3DI	GCAATG	3 cut(s)	934, 1028, 1781
BseBI	CCWGG	4 cut(s)	343, 1405, 1623, 1710
BseDI	CCNNGG	7 cut(s)	144, 627, 1016, 1621, 1644, 1677, 1708
BseGI	GGATG	5 cut(s)	358, 706, 740, 1076, 1725
BseLI	CCNNNNNNNGG	7 cut(s)	34, 144, 145, 291, 342, 750, 1160
BseMI	GCAATG	3 cut(s)	934, 1028, 1781
BseMII	CTCAG	5 cut(s)	22, 588, 1439, 1689, 1776
BseNI	ACTGG	6 cut(s)	132, 722, 1031, 1236, 1349, 1732
BseRI	GAGGAG	2 cut(s)	644, 998
BseSI	GKGCMC	3 cut(s)	162, 897, 1109
BseXI	GCAGC	3 cut(s)	368, 469, 588
BseYI	CCCAGC	1 cut(s)	1081
Bsh1236I	CGCG	2 cut(s)	14, 56
Bsh1285I	CGRYCG	1 cut(s)	1154
BshFI	GGCC	5 cut(s)	41, 754, 1107, 1403, 1629
BshNI	GGYRCC	4 cut(s)	167, 865, 1364, 1648
BshTI	ACCGGT	1 cut(s)	1153
BsiEI	CGRYCG	1 cut(s)	1154
BsiHKCI	CYCGRG	1 cut(s)	144
BsiSI	CCGG	8 cut(s)	94, 139, 145, 162, 306, 336, 1154, 1589
BslFI	GGGAC	2 cut(s)	161, 488
BslI	CCNNNNNNNGG	7 cut(s)	34, 144, 145, 291, 342, 750, 1160
BsmAI	GTCTC	2 cut(s)	190, 1255
BsmBI	CGTCTC	1 cut(s)	190
BsmFI	GGGAC	2 cut(s)	161, 488
BsmI	GAATGC	1 cut(s)	494
BsnI	GGCC	5 cut(s)	41, 754, 1107, 1403, 1629
BsoBI	CYCGRG	1 cut(s)	144
Bsp119I	TTCGAA	3 cut(s)	805, 1305, 1486
Bsp120I	GGGCCC	1 cut(s)	1105
Bsp1286I	GDGCHC	5 cut(s)	162, 897, 1030, 1109, 1275
Bsp143I	GATC	7 cut(s)	88, 130, 422, 1042, 1258, 1490, 1545
Bsp1720I	GCTNAGC	1 cut(s)	597
BspACI	CCGC	6 cut(s)	29, 38, 56, 165, 492, 1063
BspANI	GGCC	5 cut(s)	41, 754, 1107, 1403, 1629
BspCNI	CTCAG	5 cut(s)	21, 589, 1440, 1690, 1775
BspFNI	CGCG	2 cut(s)	14, 56
BspMAI	CTGCAG	1 cut(s)	606
BspPI	GGATC	2 cut(s)	125, 138
BspT104I	TTCGAA	3 cut(s)	805, 1305, 1486
BspT107I	GGYRCC	4 cut(s)	167, 865, 1364, 1648
BsrDI	GCAATG	3 cut(s)	934, 1028, 1781
BsrFI	RCCGGY	3 cut(s)	335, 1153, 1588
BsrI	ACTGG	6 cut(s)	132, 722, 1031, 1236, 1349, 1732
BssAI	RCCGGY	3 cut(s)	335, 1153, 1588
BssECI	CCNNGG	7 cut(s)	144, 627, 1016, 1621, 1644, 1677, 1708
BssMI	GATC	7 cut(s)	88, 130, 422, 1042, 1258, 1490, 1545
BssNAI	GTATAC	1 cut(s)	1036
BssNI	GRCGYC	3 cut(s)	168, 237, 866
BssT1I	CCWWGG	2 cut(s)	1644, 1677
Bst1107I	GTATAC	1 cut(s)	1036
Bst2UI	CCWGG	4 cut(s)	343, 1405, 1623, 1710
Bst4CI	ACNGT	3 cut(s)	429, 1468, 1557
BstACI	GRCGYC	3 cut(s)	168, 237, 866
BstBI	TTCGAA	3 cut(s)	805, 1305, 1486
BstC8I	GCNNGC	3 cut(s)	215, 594, 1369
BstDEI	CTNAG	9 cut(s)	8, 597, 938, 962, 1000, 1058, 1448, 1698, 1762
BstDSI	CCRYGG	1 cut(s)	1016
BstEII	GGTNACC	2 cut(s)	1193, 1702
BstENI	CCTNNNNNAGG	1 cut(s)	748
BstF5I	GGATG	5 cut(s)	358, 706, 740, 1076, 1725
BstFNI	CGCG	2 cut(s)	14, 56
BstH2I	RGCGCY	2 cut(s)	171, 869
BstHHI	GCGC	4 cut(s)	14, 170, 219, 868
BstKTI	GATC	7 cut(s)	91, 133, 425, 1045, 1261, 1493, 1548
BstMAI	GTCTC	2 cut(s)	190, 1255
BstMBI	GATC	7 cut(s)	88, 130, 422, 1042, 1258, 1490, 1545
BstMCI	CGRYCG	1 cut(s)	1154
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 28, 47, 56, 210, 488, 491, 598, 1082
BstNI	CCWGG	4 cut(s)	343, 1405, 1623, 1710
BstNSI	RCATGY	2 cut(s)	1076, 1337
BstPI	GGTNACC	2 cut(s)	1193, 1702
BstSCI	CCNGG	8 cut(s)	137, 143, 144, 160, 341, 1403, 1621, 1708
BstSFI	CTRYAG	1 cut(s)	602
BstSLI	GKGCMC	3 cut(s)	162, 897, 1109
BstUI	CGCG	2 cut(s)	14, 56
BstV1I	GCAGC	3 cut(s)	368, 469, 588
BstV2I	GAAGAC	2 cut(s)	1075, 1809
BstX2I	RGATCY	1 cut(s)	130
BstXI	CCANNNNNNTGG	2 cut(s)	1023, 1716
BstYI	RGATCY	1 cut(s)	130
BstZ17I	GTATAC	1 cut(s)	1036
Bsu36I	CCTNAGG	2 cut(s)	1448, 1698
BsuRI	GGCC	5 cut(s)	41, 754, 1107, 1403, 1629
BtgI	CCRYGG	1 cut(s)	1016
BtrI	CACGTC	1 cut(s)	460
BtsCI	GGATG	5 cut(s)	358, 706, 740, 1076, 1725
BtsI	GCAGTG	2 cut(s)	1341, 1620
BtsIMutI	CAGTG	4 cut(s)	715, 1254, 1341, 1620
Cac8I	GCNNGC	3 cut(s)	215, 594, 1369
CfoI	GCGC	4 cut(s)	14, 170, 219, 868
Cfr10I	RCCGGY	3 cut(s)	335, 1153, 1588
Cfr13I	GGNCC	8 cut(s)	40, 141, 148, 475, 1105, 1106, 1628, 1694
Cfr9I	CCCGGG	1 cut(s)	144
Csp6I	GTAC	7 cut(s)	16, 268, 513, 958, 1174, 1563, 1618
CspAI	ACCGGT	1 cut(s)	1153
CviAII	CATG	3 cut(s)	944, 1073, 1334
CviQI	GTAC	7 cut(s)	16, 268, 513, 958, 1174, 1563, 1618
DdeI	CTNAG	9 cut(s)	8, 597, 938, 962, 1000, 1058, 1448, 1698, 1762
DinI	GGCGCC	2 cut(s)	169, 867
DpnI	GATC	7 cut(s)	90, 132, 424, 1044, 1260, 1492, 1547
DpnII	GATC	7 cut(s)	88, 130, 422, 1042, 1258, 1490, 1545
EciI	GGCGGA	1 cut(s)	1078
Eco130I	CCWWGG	2 cut(s)	1644, 1677
Eco147I	AGGCCT	1 cut(s)	1403
Eco24I	GRGCYC	3 cut(s)	1030, 1109, 1275
Eco47I	GGWCC	4 cut(s)	141, 148, 475, 1694
Eco57I	CTGAAG	1 cut(s)	1199
Eco81I	CCTNAGG	2 cut(s)	1448, 1698
Eco88I	CYCGRG	1 cut(s)	144
Eco91I	GGTNACC	2 cut(s)	1193, 1702
EcoNI	CCTNNNNNAGG	1 cut(s)	748
EcoO109I	RGGNCCY	4 cut(s)	148, 475, 1106, 1694
EcoO65I	GGTNACC	2 cut(s)	1193, 1702
EcoRI	GAATTC	2 cut(s)	100, 1606
EcoRII	CCWGG	4 cut(s)	341, 1403, 1621, 1708
EcoT14I	CCWWGG	2 cut(s)	1644, 1677
EcoT22I	ATGCAT	1 cut(s)	1078
EcoT38I	GRGCYC	3 cut(s)	1030, 1109, 1275
EgeI	GGCGCC	2 cut(s)	169, 867
EheI	GGCGCC	2 cut(s)	169, 867
ErhI	CCWWGG	2 cut(s)	1644, 1677
Esp3I	CGTCTC	1 cut(s)	190
FaeI	CATG	3 cut(s)	947, 1076, 1337
FaqI	GGGAC	2 cut(s)	161, 488
FatI	CATG	3 cut(s)	943, 1072, 1333
FauI	CCCGC	1 cut(s)	36
FbaI	TGATCA	2 cut(s)	1258, 1545
FblI	GTMKAC	1 cut(s)	1035
Fnu4HI	GCNGC	7 cut(s)	39, 57, 166, 357, 483, 492, 602
FokI	GGATG	5 cut(s)	365, 713, 727, 1063, 1732
FriOI	GRGCYC	3 cut(s)	1030, 1109, 1275
Fsp4HI	GCNGC	7 cut(s)	39, 57, 166, 357, 483, 492, 602
FspBI	CTAG	1 cut(s)	1479
GlaI	GCGC	4 cut(s)	13, 169, 218, 867
GluI	GCNGC	7 cut(s)	39, 57, 166, 357, 483, 492, 602
GsaI	CCCAGC	1 cut(s)	1085
GsuI	CTGGAG	2 cut(s)	1014, 1749
HaeII	RGCGCY	2 cut(s)	171, 869
HaeIII	GGCC	5 cut(s)	41, 754, 1107, 1403, 1629
HapII	CCGG	8 cut(s)	94, 139, 145, 162, 306, 336, 1154, 1589
HhaI	GCGC	4 cut(s)	14, 170, 219, 868
Hin1I	GRCGYC	3 cut(s)	168, 237, 866
Hin1II	CATG	3 cut(s)	947, 1076, 1337
Hin6I	GCGC	4 cut(s)	12, 168, 217, 866
HinP1I	GCGC	4 cut(s)	12, 168, 217, 866
HincII	GTYRAC	2 cut(s)	432, 1123
HindII	GTYRAC	2 cut(s)	432, 1123
HindIII	AAGCTT	1 cut(s)	1736
HpaI	GTTAAC	2 cut(s)	432, 1123
HpaII	CCGG	8 cut(s)	94, 139, 145, 162, 306, 336, 1154, 1589
HphI	GGTGA	4 cut(s)	544, 1205, 1696, 1783
Hpy166II	GTNNAC	5 cut(s)	432, 559, 1036, 1123, 1618
Hpy188I	TCNGA	6 cut(s)	135, 235, 255, 262, 442, 1495
Hpy188III	TCNNGA	3 cut(s)	1447, 1527, 1697
Hpy8I	GTNNAC	5 cut(s)	432, 559, 1036, 1123, 1618
Hpy99I	CGWCG	7 cut(s)	56, 158, 176, 239, 242, 1299, 1666
HpyAV	CCTTC	6 cut(s)	343, 474, 801, 1126, 1175, 1496
HpyCH4III	ACNGT	3 cut(s)	429, 1468, 1557
HpyCH4IV	ACGT	6 cut(s)	183, 237, 459, 519, 1297, 1664
HpyCH4V	TGCA	7 cut(s)	356, 604, 947, 1076, 1341, 1371, 1568
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 28, 47, 56, 210, 488, 491, 598, 1082
HpyF3I	CTNAG	9 cut(s)	8, 597, 938, 962, 1000, 1058, 1448, 1698, 1762
HpySE526I	ACGT	6 cut(s)	183, 237, 459, 519, 1297, 1664
Hsp92I	GRCGYC	3 cut(s)	168, 237, 866
Hsp92II	CATG	3 cut(s)	947, 1076, 1337
HspAI	GCGC	4 cut(s)	12, 168, 217, 866
KasI	GGCGCC	2 cut(s)	167, 865
KflI	GGGWCCC	1 cut(s)	475
Ksp22I	TGATCA	2 cut(s)	1258, 1545
KspAI	GTTAAC	2 cut(s)	432, 1123
Kzo9I	GATC	7 cut(s)	88, 130, 422, 1042, 1258, 1490, 1545
LmnI	GCTCC	8 cut(s)	236, 344, 364, 442, 985, 1033, 1320, 1730
Lsp1109I	GCAGC	3 cut(s)	368, 469, 588
LweI	GCATC	5 cut(s)	343, 749, 1085, 1228, 1481
MaeI	CTAG	1 cut(s)	1479
MaeII	ACGT	6 cut(s)	183, 237, 459, 519, 1297, 1664
MalI	GATC	7 cut(s)	90, 132, 424, 1044, 1260, 1492, 1547
MboI	GATC	7 cut(s)	88, 130, 422, 1042, 1258, 1490, 1545
MboII	GAAGA	6 cut(s)	547, 1080, 1205, 1223, 1500, 1814
MflI	RGATCY	1 cut(s)	130
MhlI	GDGCHC	5 cut(s)	162, 897, 1030, 1109, 1275
Mly113I	GGCGCC	2 cut(s)	168, 866
MlyI	GAGTC	2 cut(s)	1373, 1799
MmeI	TCCRAC	3 cut(s)	158, 258, 1779
Mph1103I	ATGCAT	1 cut(s)	1078
MseI	TTAA	9 cut(s)	287, 315, 327, 392, 431, 614, 726, 1122, 1374
MslI	CAYNNNNRTG	4 cut(s)	881, 1021, 1266, 1336
MspI	CCGG	8 cut(s)	94, 139, 145, 162, 306, 336, 1154, 1589
MspR9I	CCNGG	8 cut(s)	139, 145, 146, 162, 343, 1405, 1623, 1710
Mva1269I	GAATGC	1 cut(s)	494
MvaI	CCWGG	4 cut(s)	343, 1405, 1623, 1710
MvnI	CGCG	2 cut(s)	14, 56
MwoI	GCNNNNNNNGC	9 cut(s)	11, 28, 47, 56, 210, 488, 491, 598, 1082
NarI	GGCGCC	2 cut(s)	168, 866
NciI	CCSGG	4 cut(s)	139, 145, 146, 162
NdeII	GATC	7 cut(s)	88, 130, 422, 1042, 1258, 1490, 1545
NlaIII	CATG	3 cut(s)	947, 1076, 1337
NmeAIII	GCCGAG	1 cut(s)	652
NmuCI	GTSAC	4 cut(s)	1193, 1393, 1582, 1702
NsiI	ATGCAT	1 cut(s)	1078
NspI	RCATGY	2 cut(s)	1076, 1337
NspV	TTCGAA	3 cut(s)	805, 1305, 1486
PceI	AGGCCT	1 cut(s)	1403
PciI	ACATGT	1 cut(s)	1333
PcsI	WCGNNNNNNNCGW	1 cut(s)	180
PctI	GAATGC	1 cut(s)	494
PfeI	GAWTC	8 cut(s)	656, 933, 1139, 1164, 1483, 1530, 1573, 1758
PflFI	GACNNNGTC	1 cut(s)	152
PinAI	ACCGGT	1 cut(s)	1153
PkrI	GCNGC	7 cut(s)	40, 58, 167, 358, 484, 493, 603
PleI	GAGTC	2 cut(s)	1373, 1799
PluTI	GGCGCC	2 cut(s)	171, 869
PpsI	GAGTC	2 cut(s)	1373, 1799
PpuMI	RGGWCCY	3 cut(s)	148, 475, 1694
PscI	ACATGT	1 cut(s)	1333
PshBI	ATTAAT	1 cut(s)	1374
Psp5II	RGGWCCY	3 cut(s)	148, 475, 1694
Psp6I	CCWGG	4 cut(s)	341, 1403, 1621, 1708
PspEI	GGTNACC	2 cut(s)	1193, 1702
PspFI	CCCAGC	1 cut(s)	1081
PspGI	CCWGG	4 cut(s)	341, 1403, 1621, 1708
PspOMI	GGGCCC	1 cut(s)	1105
PspPI	GGNCC	8 cut(s)	40, 141, 148, 475, 1105, 1106, 1628, 1694
PspPPI	RGGWCCY	3 cut(s)	148, 475, 1694
PstI	CTGCAG	1 cut(s)	606
PsuI	RGATCY	1 cut(s)	130
PsyI	GACNNNGTC	1 cut(s)	152
RsaI	GTAC	7 cut(s)	17, 269, 514, 959, 1175, 1564, 1619
RsaNI	GTAC	7 cut(s)	16, 268, 513, 958, 1174, 1563, 1618
RseI	CAYNNNNRTG	4 cut(s)	881, 1021, 1266, 1336
SaqAI	TTAA	9 cut(s)	287, 315, 327, 392, 431, 614, 726, 1122, 1374
SatI	GCNGC	7 cut(s)	39, 57, 166, 357, 483, 492, 602
Sau3AI	GATC	7 cut(s)	88, 130, 422, 1042, 1258, 1490, 1545
Sau96I	GGNCC	8 cut(s)	40, 141, 148, 475, 1105, 1106, 1628, 1694
ScaI	AGTACT	1 cut(s)	1564
SchI	GAGTC	2 cut(s)	1373, 1799
ScrFI	CCNGG	8 cut(s)	139, 145, 146, 162, 343, 1405, 1623, 1710
SduI	GDGCHC	5 cut(s)	162, 897, 1030, 1109, 1275
SfaNI	GCATC	5 cut(s)	343, 749, 1085, 1228, 1481
SfcI	CTRYAG	1 cut(s)	602
SfoI	GGCGCC	2 cut(s)	169, 867
SfuI	TTCGAA	3 cut(s)	805, 1305, 1486
SinI	GGWCC	4 cut(s)	141, 148, 475, 1694
SmaI	CCCGGG	1 cut(s)	146
SmiMI	CAYNNNNRTG	4 cut(s)	881, 1021, 1266, 1336
SmlI	CTYRAG	2 cut(s)	587, 1507
SmoI	CTYRAG	2 cut(s)	587, 1507
SseBI	AGGCCT	1 cut(s)	1403
SsiI	CCGC	6 cut(s)	29, 38, 56, 165, 492, 1063
SspDI	GGCGCC	2 cut(s)	167, 865
SspMI	CTAG	1 cut(s)	1479
StuI	AGGCCT	1 cut(s)	1403
StyD4I	CCNGG	8 cut(s)	137, 143, 144, 160, 341, 1403, 1621, 1708
StyI	CCWWGG	2 cut(s)	1644, 1677
TaaI	ACNGT	3 cut(s)	429, 1468, 1557
TaiI	ACGT	6 cut(s)	186, 240, 462, 522, 1300, 1667
TaqI	TCGA	8 cut(s)	24, 805, 851, 1150, 1305, 1486, 1756, 1808
TatI	WGTACW	2 cut(s)	267, 1562
TauI	GCSGC	4 cut(s)	41, 59, 168, 494
TfiI	GAWTC	8 cut(s)	656, 933, 1139, 1164, 1483, 1530, 1573, 1758
Tru1I	TTAA	9 cut(s)	287, 315, 327, 392, 431, 614, 726, 1122, 1374
Tru9I	TTAA	9 cut(s)	287, 315, 327, 392, 431, 614, 726, 1122, 1374
TscAI	CASTG	4 cut(s)	722, 1261, 1348, 1620
TseFI	GTSAC	4 cut(s)	1193, 1393, 1582, 1702
TseI	GCWGC	3 cut(s)	356, 482, 601
Tsp45I	GTSAC	4 cut(s)	1193, 1393, 1582, 1702
TspDTI	ATGAA	5 cut(s)	487, 1145, 1230, 1522, 1770
TspGWI	ACGGA	3 cut(s)	291, 1132, 1157
TspMI	CCCGGG	1 cut(s)	144
TspRI	CASTG	4 cut(s)	722, 1261, 1348, 1620
Tth111I	GACNNNGTC	1 cut(s)	152
VpaK11BI	GGWCC	4 cut(s)	141, 148, 475, 1694
VspI	ATTAAT	1 cut(s)	1374
XagI	CCTNNNNNAGG	1 cut(s)	748
XapI	RAATTY	6 cut(s)	100, 297, 1004, 1457, 1600, 1606
XceI	RCATGY	2 cut(s)	1076, 1337
XmaI	CCCGGG	1 cut(s)	144
XmiI	GTMKAC	1 cut(s)	1035
XspI	CTAG	1 cut(s)	1479
ZraI	GACGTC	1 cut(s)	238
ZrmI	AGTACT	1 cut(s)	1564
Zsp2I	ATGCAT	1 cut(s)	1078

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.