Rh5BG066500

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010968
Physical Location & Seq
Forward (+)
5484113 .. 5488379
4267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG066500.1

Sequence Viewer

Length: 1290 bp
ATGGGTGGAAAGGATATAGTTCACGTAAAGCAGTTACAAAACTCAAAACTTGAGCCCACTGAACTGCAGAAATTGTTAAAGCAATTAGCTGAAGAGAGATTTTCTGATAATGCAAATGAATCTTCTATATCAAATACTGCTGATCTATTAGGAAAAATGAGGGATGACCATCCTGTTCACTGGGATATTCAGAGAACATTAGCATCCTCAGTAAGGCCACCACCCATCATCACTCACTCAAGGAATGATGATATTGTTACCATTAGTGTTCGAAGGGGAGGTGTTGATGTTGGTCAAAGTCATAACCAATGGCTTTCGACTATATCACAGGCGCCTGATGCCATATCAATGTCCTTTGTGCCCATAACATCACTTTTGGGTGGTATTCAAGGCAATGGATTTCTTAGCCATGCAGTAAATCTGTATCTTAGATACAAACCACCAATAGAGGAGCTTCAACAATTCTTAGAATTTCAATTACCACGGCAATGGGCTCCAGTATACGCTGATCTGCCTCTTGGTCTAAGGCGGAGAAGACATGCATCCCCATCACTTCAGTTCAGTTTTATGGGCCCCAAGCTATACGTTAACACTATGAAGGTTGATTCCGTAAATCGACCGGTTACAGGAATCCGTTTGTACCTTGAAGGTAAAAAAGGTGACCATATGGCAATCCATCTTCAGCATCTTACAAGTCTTCCCAGTTCCTTCCAAGTCTCAACTGATCACAATTATGAGCCCATTGACGAGCCAGAGGAACGAGGCTACTTTGAACCTGTCAAGTGGAGCATATTTTCACATGTATGCACTGCCCCAGTACAGTATAATGGTGCCTGCATCAATGACTCTGCGTCTATTGTGACAAAGGCCTGGTTTGAAGTCAAGGCTATTGGAATGAGGAAAGTTTTGTTCCTGAGGCTTGGATTTTCTACTGTGGCATCTGCAAGGATTCGAAGATCAGAATGGGAAGGCTCAAGCTCATCACGTAAGTCAGGATTCATCTCAACCTTGATCAGCACAAGGCTTAGTGCTGCGCTCAATCCACCTGTGGCAGCACCACCAAAAGTGGATTTGAATTCAGCAGTGTATCCTGGTGGCCCACCTTTACCTTCCAAGGCACCAAAAATGGCGACGTTTGTAGATACCAAGGAAATGTTGAGAGGTCCTGAGGACACCCCAGGATATTGGATGGTTACCGGAGCCAAGCTTTGTGTAGAGGGTGGTCGAATCTCAGTCAAAGTGAAGTATTCATTGCTGACAATTATGTCAGAAGACTCGATGATGTTTTAG

Protein Analysis

429

Amino Acids

47.7

Weight (kDa)

9.15

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 1 - 142 7.5e-11 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010968)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28380
fragaria_vesca FvH4_3g08740
malus_domestica MD05G1311300.v1.1 MD10G1290300.v1.1
prunus_persica Prupe.4G051500_v2.0.a1
pyrus_communis pycom05g28740 pycom10g24250 pycom10g24280
rosa_chinensis RchiOBHm_Chr5g0009001
rosa_laevigata RLG00000031624
rosa_multiflora Rmu_sc0021361.1_g000001
rosa_roxburghii Rroxscaffold_1G00067010 Rroxscaffold_1G00067650
rosa_rugosa Rorug04G0440800 Rorug04G0440900
rosa_samantha Rh5AG069800 Rh5BG066500 Rh5CG077800 Rh5DG065800
rosa_wichuraiana Rw5G006510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1264
AccB1I GGYRCC 3 cut(s) 331, 830, 1117
AccI GTMKAC 1 cut(s) 501
AciI CCGC 1 cut(s) 529
AcsI RAATTY 2 cut(s) 470, 1075
AcuI CTGAAG 3 cut(s) 111, 539, 665
AcyI GRCGYC 1 cut(s) 332
AfaI GTAC 2 cut(s) 641, 819
AfiI CCNNNNNNNGG 2 cut(s) 213, 626
AflIII ACRYGT 1 cut(s) 799
AgeI ACCGGT 1 cut(s) 619
AgsI TTSAA 7 cut(s) 389, 458, 476, 647, 773, 878, 1075
AhdI GACNNNNNGTC 1 cut(s) 850
AjnI CCWGG 3 cut(s) 869, 1090, 1177
AluBI AGCT 5 cut(s) 89, 454, 580, 978, 1207
AluI AGCT 5 cut(s) 89, 454, 580, 978, 1207
Alw26I GTCTC 1 cut(s) 721
AoxI GGCC 4 cut(s) 215, 571, 867, 1096
ApaI GGGCCC 1 cut(s) 575
ApeKI GCWGC 2 cut(s) 1031, 1052
ApoI RAATTY 2 cut(s) 470, 1075
AsiGI ACCGGT 1 cut(s) 619
AspLEI GCGC 2 cut(s) 334, 1036
AspS9I GGNCC 4 cut(s) 571, 572, 1097, 1163
AsuHPI GGTGA 1 cut(s) 671
AsuII TTCGAA 2 cut(s) 271, 952
AvaII GGWCC 1 cut(s) 1163
AxyI CCTNAGG 2 cut(s) 914, 1167
BaeGI GKGCMC 2 cut(s) 363, 575
BanI GGYRCC 3 cut(s) 331, 830, 1117
BanII GRGCYC 4 cut(s) 57, 496, 575, 741
BbsI GAAGAC 3 cut(s) 541, 689, 1278
BbvI GCAGC 2 cut(s) 1018, 1064
BccI CCATC 5 cut(s) 177, 233, 556, 684, 1183
BceAI ACGGC 1 cut(s) 500
BciT130I CCWGG 3 cut(s) 871, 1092, 1179
BciVI GTATCC 1 cut(s) 1098
BclI TGATCA 2 cut(s) 724, 1011
BcoDI GTCTC 1 cut(s) 721
BfmI CTRYAG 1 cut(s) 65
BfoI RGCGCY 1 cut(s) 335
BfuI GTATCC 1 cut(s) 1098
BisI GCNGC 2 cut(s) 1032, 1053
BlsI GCNGC 2 cut(s) 1033, 1054
Bme1390I CCNGG 3 cut(s) 871, 1092, 1179
Bme18I GGWCC 1 cut(s) 1163
BmeRI GACNNNNNGTC 1 cut(s) 850
BmgT120I GGNCC 4 cut(s) 571, 572, 1097, 1163
BmiI GGNNCC 7 cut(s) 333, 495, 573, 574, 832, 1119, 1201
BmrFI CCNGG 3 cut(s) 871, 1092, 1179
BmrI ACTGGG 3 cut(s) 190, 696, 809
BmsI GCATC 6 cut(s) 212, 328, 551, 694, 846, 947
BmuI ACTGGG 3 cut(s) 190, 696, 809
BpiI GAAGAC 3 cut(s) 541, 689, 1278
BpmI CTGGAG 1 cut(s) 480
Bpu14I TTCGAA 2 cut(s) 271, 952
BpuEI CTTGAG 3 cut(s) 71, 223, 958
BsaAI YACGTR 2 cut(s) 25, 986
BsaBI GATNNNNATC 1 cut(s) 168
BsaHI GRCGYC 1 cut(s) 332
BsaJI CCNNGG 4 cut(s) 482, 1113, 1146, 1177
BsaWI WCCGGW 2 cut(s) 619, 1196
Bsc4I CCNNNNNNNGG 2 cut(s) 213, 626
Bse118I RCCGGY 1 cut(s) 619
Bse1I ACTGG 4 cut(s) 185, 497, 702, 815
Bse21I CCTNAGG 2 cut(s) 914, 1167
Bse3DI GCAATG 3 cut(s) 400, 494, 1250
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 3 cut(s) 871, 1092, 1179
BseDI CCNNGG 4 cut(s) 482, 1113, 1146, 1177
BseGI GGATG 5 cut(s) 169, 169, 203, 542, 1194
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 2 cut(s) 213, 626
BseMI GCAATG 3 cut(s) 400, 494, 1250
BseMII CTCAG 4 cut(s) 222, 905, 1158, 1245
BseNI ACTGG 4 cut(s) 185, 497, 702, 815
BseRI GAGGAG 1 cut(s) 464
BseSI GKGCMC 2 cut(s) 363, 575
BseXI GCAGC 2 cut(s) 1018, 1064
Bsh1285I CGRYCG 1 cut(s) 620
BshFI GGCC 4 cut(s) 217, 573, 869, 1098
BshNI GGYRCC 3 cut(s) 331, 830, 1117
BshTI ACCGGT 1 cut(s) 619
BsiEI CGRYCG 1 cut(s) 620
BsiSI CCGG 2 cut(s) 620, 1197
BslI CCNNNNNNNGG 2 cut(s) 213, 626
BsmAI GTCTC 1 cut(s) 721
BsnI GGCC 4 cut(s) 217, 573, 869, 1098
Bsp119I TTCGAA 2 cut(s) 271, 952
Bsp120I GGGCCC 1 cut(s) 571
Bsp1286I GDGCHC 5 cut(s) 57, 363, 496, 575, 741
Bsp143I GATC 5 cut(s) 142, 508, 724, 956, 1011
BspACI CCGC 1 cut(s) 529
BspANI GGCC 4 cut(s) 217, 573, 869, 1098
BspCNI CTCAG 4 cut(s) 221, 906, 1159, 1244
BspLI GGNNCC 7 cut(s) 333, 495, 573, 574, 832, 1119, 1201
BspMAI CTGCAG 1 cut(s) 69
BspT104I TTCGAA 2 cut(s) 271, 952
BspT107I GGYRCC 3 cut(s) 331, 830, 1117
BsrDI GCAATG 3 cut(s) 400, 494, 1250
BsrFI RCCGGY 1 cut(s) 619
BsrI ACTGG 4 cut(s) 185, 497, 702, 815
BssAI RCCGGY 1 cut(s) 619
BssECI CCNNGG 4 cut(s) 482, 1113, 1146, 1177
BssMI GATC 5 cut(s) 142, 508, 724, 956, 1011
BssNAI GTATAC 1 cut(s) 502
BssNI GRCGYC 1 cut(s) 332
BssT1I CCWWGG 2 cut(s) 1113, 1146
Bst1107I GTATAC 1 cut(s) 502
Bst2UI CCWGG 3 cut(s) 871, 1092, 1179
Bst4CI ACNGT 2 cut(s) 822, 934
Bst6I CTCTTC 1 cut(s) 87
BstACI GRCGYC 1 cut(s) 332
BstBAI YACGTR 2 cut(s) 25, 986
BstBI TTCGAA 2 cut(s) 271, 952
BstC8I GCNNGC 1 cut(s) 835
BstDEI CTNAG 9 cut(s) 208, 404, 428, 466, 524, 914, 1025, 1167, 1231
BstDSI CCRYGG 1 cut(s) 482
BstEII GGTNACC 2 cut(s) 659, 1192
BstENI CCTNNNNNAGG 1 cut(s) 211
BstF5I GGATG 5 cut(s) 169, 169, 203, 542, 1194
BstH2I RGCGCY 1 cut(s) 335
BstHHI GCGC 2 cut(s) 334, 1036
BstKTI GATC 5 cut(s) 145, 511, 727, 959, 1014
BstMAI GTCTC 1 cut(s) 721
BstMBI GATC 5 cut(s) 142, 508, 724, 956, 1011
BstMCI CGRYCG 1 cut(s) 620
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 3 cut(s) 871, 1092, 1179
BstNSI RCATGY 2 cut(s) 542, 803
BstPI GGTNACC 2 cut(s) 659, 1192
BstSCI CCNGG 3 cut(s) 869, 1090, 1177
BstSFI CTRYAG 1 cut(s) 65
BstSLI GKGCMC 2 cut(s) 363, 575
BstV1I GCAGC 2 cut(s) 1018, 1064
BstV2I GAAGAC 3 cut(s) 541, 689, 1278
BstXI CCANNNNNNTGG 2 cut(s) 489, 1185
BstZ17I GTATAC 1 cut(s) 502
Bsu36I CCTNAGG 2 cut(s) 914, 1167
BsuI GTATCC 1 cut(s) 1098
BsuRI GGCC 4 cut(s) 217, 573, 869, 1098
BtgI CCRYGG 1 cut(s) 482
BtsCI GGATG 5 cut(s) 169, 169, 203, 542, 1194
BtsI GCAGTG 2 cut(s) 807, 1089
BtsIMutI CAGTG 4 cut(s) 57, 178, 807, 1089
Cac8I GCNNGC 1 cut(s) 835
CfoI GCGC 2 cut(s) 334, 1036
Cfr10I RCCGGY 1 cut(s) 619
Cfr13I GGNCC 4 cut(s) 571, 572, 1097, 1163
CseI GACGC 1 cut(s) 840
Csp6I GTAC 2 cut(s) 640, 818
CspAI ACCGGT 1 cut(s) 619
CviAII CATG 3 cut(s) 410, 539, 800
CviQI GTAC 2 cut(s) 640, 818
DdeI CTNAG 9 cut(s) 208, 404, 428, 466, 524, 914, 1025, 1167, 1231
DinI GGCGCC 1 cut(s) 333
DpnI GATC 5 cut(s) 144, 510, 726, 958, 1013
DpnII GATC 5 cut(s) 142, 508, 724, 956, 1011
DrdI GACNNNNNNGTC 1 cut(s) 1264
DriI GACNNNNNGTC 1 cut(s) 850
DseDI GACNNNNNNGTC 1 cut(s) 1264
Eam1104I CTCTTC 1 cut(s) 87
Eam1105I GACNNNNNGTC 1 cut(s) 850
EarI CTCTTC 1 cut(s) 87
EciI GGCGGA 1 cut(s) 544
Eco130I CCWWGG 2 cut(s) 1113, 1146
Eco147I AGGCCT 1 cut(s) 869
Eco24I GRGCYC 4 cut(s) 57, 496, 575, 741
Eco47I GGWCC 1 cut(s) 1163
Eco57I CTGAAG 3 cut(s) 111, 539, 665
Eco81I CCTNAGG 2 cut(s) 914, 1167
Eco91I GGTNACC 2 cut(s) 659, 1192
EcoNI CCTNNNNNAGG 1 cut(s) 211
EcoO109I RGGNCCY 2 cut(s) 572, 1163
EcoO65I GGTNACC 2 cut(s) 659, 1192
EcoRI GAATTC 1 cut(s) 1075
EcoRII CCWGG 3 cut(s) 869, 1090, 1177
EcoT14I CCWWGG 2 cut(s) 1113, 1146
EcoT22I ATGCAT 1 cut(s) 544
EcoT38I GRGCYC 4 cut(s) 57, 496, 575, 741
EgeI GGCGCC 1 cut(s) 333
EheI GGCGCC 1 cut(s) 333
ErhI CCWWGG 2 cut(s) 1113, 1146
FaeI CATG 3 cut(s) 413, 542, 803
FatI CATG 3 cut(s) 409, 538, 799
FauNDI CATATG 1 cut(s) 666
FbaI TGATCA 2 cut(s) 724, 1011
FblI GTMKAC 1 cut(s) 501
Fnu4HI GCNGC 2 cut(s) 1032, 1053
FokI GGATG 5 cut(s) 156, 176, 190, 529, 1201
FriOI GRGCYC 4 cut(s) 57, 496, 575, 741
Fsp4HI GCNGC 2 cut(s) 1032, 1053
GlaI GCGC 2 cut(s) 333, 1035
GluI GCNGC 2 cut(s) 1032, 1053
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 335
HaeIII GGCC 4 cut(s) 217, 573, 869, 1098
HapII CCGG 2 cut(s) 620, 1197
HgaI GACGC 1 cut(s) 840
HhaI GCGC 2 cut(s) 334, 1036
Hin1I GRCGYC 1 cut(s) 332
Hin1II CATG 3 cut(s) 413, 542, 803
Hin6I GCGC 2 cut(s) 332, 1034
HinP1I GCGC 2 cut(s) 332, 1034
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 1205
HinfI GANTC 8 cut(s) 119, 605, 630, 845, 949, 996, 1227, 1274
HpaI GTTAAC 1 cut(s) 589
HpaII CCGG 2 cut(s) 620, 1197
HphI GGTGA 1 cut(s) 671
Hpy166II GTNNAC 4 cut(s) 22, 178, 502, 589
Hpy188I TCNGA 4 cut(s) 106, 192, 961, 1270
Hpy188III TCNNGA 3 cut(s) 913, 993, 1166
Hpy8I GTNNAC 4 cut(s) 22, 178, 502, 589
Hpy99I CGWCG 1 cut(s) 1135
HpyAV CCTTC 6 cut(s) 267, 592, 641, 718, 962, 1119
HpyCH4III ACNGT 2 cut(s) 822, 934
HpyCH4IV ACGT 4 cut(s) 24, 585, 985, 1133
HpyCH4V TGCA 7 cut(s) 67, 113, 413, 542, 807, 837, 944
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 9 cut(s) 208, 404, 428, 466, 524, 914, 1025, 1167, 1231
HpySE526I ACGT 4 cut(s) 24, 585, 985, 1133
Hsp92I GRCGYC 1 cut(s) 332
Hsp92II CATG 3 cut(s) 413, 542, 803
HspAI GCGC 2 cut(s) 332, 1034
KasI GGCGCC 1 cut(s) 331
Ksp22I TGATCA 2 cut(s) 724, 1011
KspAI GTTAAC 1 cut(s) 589
Kzo9I GATC 5 cut(s) 142, 508, 724, 956, 1011
LmnI GCTCC 4 cut(s) 451, 499, 786, 1199
Lsp1109I GCAGC 2 cut(s) 1018, 1064
LweI GCATC 6 cut(s) 212, 328, 551, 694, 846, 947
MaeII ACGT 4 cut(s) 24, 585, 985, 1133
MaeIII GTNAC 6 cut(s) 33, 256, 622, 659, 859, 1192
MalI GATC 5 cut(s) 144, 510, 726, 958, 1013
MboI GATC 5 cut(s) 142, 508, 724, 956, 1011
MboII GAAGA 7 cut(s) 104, 114, 546, 671, 689, 966, 1283
MhlI GDGCHC 5 cut(s) 57, 363, 496, 575, 741
MluCI AATT 8 cut(s) 71, 83, 461, 470, 476, 730, 1075, 1260
Mly113I GGCGCC 1 cut(s) 332
MlyI GAGTC 2 cut(s) 839, 1268
Mph1103I ATGCAT 1 cut(s) 544
MseI TTAA 2 cut(s) 77, 588
MslI CAYNNNNRTG 4 cut(s) 347, 487, 732, 802
MspI CCGG 2 cut(s) 620, 1197
MspR9I CCNGG 3 cut(s) 871, 1092, 1179
MvaI CCWGG 3 cut(s) 871, 1092, 1179
MwoI GCNNNNNNNGC 1 cut(s) 338
NarI GGCGCC 1 cut(s) 332
NdeI CATATG 1 cut(s) 666
NdeII GATC 5 cut(s) 142, 508, 724, 956, 1011
NlaIII CATG 3 cut(s) 413, 542, 803
NlaIV GGNNCC 7 cut(s) 333, 495, 573, 574, 832, 1119, 1201
NmuCI GTSAC 2 cut(s) 659, 859
NsiI ATGCAT 1 cut(s) 544
NspI RCATGY 2 cut(s) 542, 803
NspV TTCGAA 2 cut(s) 271, 952
PceI AGGCCT 1 cut(s) 869
PciI ACATGT 1 cut(s) 799
PfeI GAWTC 6 cut(s) 119, 605, 630, 949, 996, 1227
PinAI ACCGGT 1 cut(s) 619
PkrI GCNGC 2 cut(s) 1033, 1054
PleI GAGTC 2 cut(s) 839, 1268
PluTI GGCGCC 1 cut(s) 335
PpsI GAGTC 2 cut(s) 839, 1268
Ppu21I YACGTR 2 cut(s) 25, 986
PpuMI RGGWCCY 1 cut(s) 1163
PscI ACATGT 1 cut(s) 799
Psp5II RGGWCCY 1 cut(s) 1163
Psp6I CCWGG 3 cut(s) 869, 1090, 1177
PspEI GGTNACC 2 cut(s) 659, 1192
PspGI CCWGG 3 cut(s) 869, 1090, 1177
PspN4I GGNNCC 7 cut(s) 333, 495, 573, 574, 832, 1119, 1201
PspOMI GGGCCC 1 cut(s) 571
PspPI GGNCC 4 cut(s) 571, 572, 1097, 1163
PspPPI RGGWCCY 1 cut(s) 1163
PstI CTGCAG 1 cut(s) 69
RsaI GTAC 2 cut(s) 641, 819
RsaNI GTAC 2 cut(s) 640, 818
RseI CAYNNNNRTG 4 cut(s) 347, 487, 732, 802
SaqAI TTAA 2 cut(s) 77, 588
SatI GCNGC 2 cut(s) 1032, 1053
Sau3AI GATC 5 cut(s) 142, 508, 724, 956, 1011
Sau96I GGNCC 4 cut(s) 571, 572, 1097, 1163
SchI GAGTC 2 cut(s) 839, 1268
ScrFI CCNGG 3 cut(s) 871, 1092, 1179
SduI GDGCHC 5 cut(s) 57, 363, 496, 575, 741
SfaNI GCATC 6 cut(s) 212, 328, 551, 694, 846, 947
SfcI CTRYAG 1 cut(s) 65
SfoI GGCGCC 1 cut(s) 333
SfuI TTCGAA 2 cut(s) 271, 952
SinI GGWCC 1 cut(s) 1163
SmiMI CAYNNNNRTG 4 cut(s) 347, 487, 732, 802
SmlI CTYRAG 3 cut(s) 50, 238, 973
SmoI CTYRAG 3 cut(s) 50, 238, 973
Sse9I AATT 8 cut(s) 71, 83, 461, 470, 476, 730, 1075, 1260
SseBI AGGCCT 1 cut(s) 869
SsiI CCGC 1 cut(s) 529
SspDI GGCGCC 1 cut(s) 331
StuI AGGCCT 1 cut(s) 869
StyD4I CCNGG 3 cut(s) 869, 1090, 1177
StyI CCWWGG 2 cut(s) 1113, 1146
TaaI ACNGT 2 cut(s) 822, 934
TaiI ACGT 4 cut(s) 27, 588, 988, 1136
TaqI TCGA 6 cut(s) 271, 317, 616, 952, 1225, 1277
TasI AATT 8 cut(s) 71, 83, 461, 470, 476, 730, 1075, 1260
TatI WGTACW 1 cut(s) 817
TfiI GAWTC 6 cut(s) 119, 605, 630, 949, 996, 1227
Tru1I TTAA 2 cut(s) 77, 588
Tru9I TTAA 2 cut(s) 77, 588
TscAI CASTG 4 cut(s) 64, 185, 814, 1089
TseFI GTSAC 2 cut(s) 659, 859
TseI GCWGC 2 cut(s) 1031, 1052
Tsp45I GTSAC 2 cut(s) 659, 859
TspDTI ATGAA 4 cut(s) 132, 611, 988, 1239
TspGWI ACGGA 2 cut(s) 598, 623
TspRI CASTG 4 cut(s) 64, 185, 814, 1089
VpaK11BI GGWCC 1 cut(s) 1163
XagI CCTNNNNNAGG 1 cut(s) 211
XapI RAATTY 2 cut(s) 470, 1075
XceI RCATGY 2 cut(s) 542, 803
XmiI GTMKAC 1 cut(s) 501
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.