Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0010968

Physical Location & Seq

Genomic Coordinates Reverse (-)

26112890 .. 26116104

Gene Structure 3215 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g24250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1800 bp

ATGGGTCTCGCCTCGACCCCCAATCAGCTGCCCAGAATGCCGTCGCGGTCGATCGGGTCCGGGTACAATCTCTGCAACGATATCCGGCTCACCGCGTGCAAGTCTATGCTCATCGAGCTGGACTCGACCCGGACCCGCGACCTCGTCGTCCCTGGTGGTCCCGTCGTGCCCTACGTGTCCACCTCCATCAGGTGCGATAAAGGCGAGCGCACCAGGTTCCGCTCCGACGTCGTTTCGTTTAATCAGATGTCAGAGCAATACAACAAGGAAGTGTCTCTATCGGGCAAAATTCCGTCTGGTCTGTTTAATACTATGTTTAACTTCACTGGCTGCTGGCAGAAGGATGCAGCTCCGACAAAAGCTCTTGCTTTTGATGGTTGGTTGATAACTCTGTATAACATTGAGTTAGAAAGATCCCATCTAAAACTATCCCAGCATGTGATACAGGAAGTGCCTTCTACATGGGACCCTGCTGCCCTTGCCGAGTTCATTGATAAATACGGCACTCATATTGTTGTTGGGGTGAAAATGGGTGGAAAGGATATAGTTCACATGAAGCAGTTACAAAATTCAAACATTCAACCCACAGAAGTGCAGAAATTGTTAAAGCAATTAGCTGACGAGAAATTTTCTGAAGATGCAACTGGATCAGAGTCCGATGGTTTATCAGGAAAAACCAAGGATGATGACTCTATGCCCTGGGAGGTTCGTAGAATGTATGCGGCCCCAGTAAGGCAACCTACTGTCACACAAATGAATGATGGTATAGTTACTGTCTGTGTTCGAAGGGGAGGTTTTGATATTGATCAAAGTCATAACCAGTGGCTTTCAACCGTATCACAGTCACCTAATGTCATATCGATGTCCTTTGTGCCCATAACCTCTCTGTTGGGTGGTGTCCAAGGAATTGGGTTTCTAAGCCATGCAGTGAATCTCTACCTTAGATACAAACCACCGGTAGAGGAACTCCAACAATTTTTGGAATATCAGTTACCACGTCAATGGGCTCCGGTGTATGGTGATCTGCCTCTTGGTCTCAGGCGCAGAAAACAAGTATCCCCATCACTTCAATTCACCTTTATGGGCCCAAAGCTATATGTTAACACTTTAAAGGTTGATACTGGAAATCGACCGGTGACAGGAATGCGTTTGTATCTTGAAGGTAAGAAAAGTGACCATCTGGCAATCCATCTCCAGCACCTTGCAATTCTTCCCGATACTCTCCAACTCTCATACGATCACAGTTACGAGCCCATTCACGGGCCAGAGGAACGAGGCTACTTTGAACCTGTCAAGTGGAGCATATTTTCACATGTATGCACTGCCCCAGTAGAGTACAACGGGGCCTGTATTGATGACTCTGCATCAATTGTGACGAAGGCCTGGTTTGAAGCCAAGGCCATTGGAATGAGGAAAGTTTTGTTCTTGAGGCTTGGATTTTCAACGGTGGCATCTGCTAAAATCCGCAGATCAGAGTGGGAAGGACCCTCGACAACTTCTCGAAAATCAGGATTCATCTCAACCTTGATCAGCACAAGGTTTAGTACGGCATTGAATCCACCTGTGACACCAACAAAAGTGGATTTGAATTCAGCAGTTTACCCTGCAGGGCCACCTTTACCAAGCAAGGCACCAAAAATGGCGACGTTTGTAGATACCAAGGAAATGTTGAGAGGTCCTGAGGACTCGCCAGGATATTGGGTGGTCACCGGAGCCAAGCTTTGTGTAGATGGAGGCCGAATCTCGGTGAAGGTGAAGTATTCATTGTTGACAATAATGCTGGAGGATTCTATGAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008219 GO:0009626 GO:0009628 GO:0009651 GO:0009987 GO:0010337 GO:0010565 GO:0010817 GO:0012501 GO:0019222 GO:0031323 GO:0032350 GO:0033036 GO:0033037 GO:0033554 GO:0034050 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051716 GO:0052542 GO:0052545 GO:0062012 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MACPF

Protein Analysis

600

Amino Acids

66.6

Weight (kDa)

8.77

Isoelectric Point (pI)

49.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MACPF	PF01823	101 - 313	9.4e-35	MAC/Perforin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28380
fragaria_vesca	FvH4_3g08740
malus_domestica	MD05G1311300.v1.1 MD10G1290300.v1.1
prunus_persica	Prupe.4G051500_v2.0.a1
pyrus_communis	pycom05g28740 pycom10g24250 pycom10g24280
rosa_chinensis	RchiOBHm_Chr5g0009001
rosa_laevigata	RLG00000031624
rosa_multiflora	Rmu_sc0021361.1_g000001
rosa_roxburghii	Rroxscaffold_1G00067010 Rroxscaffold_1G00067650
rosa_rugosa	Rorug04G0440800 Rorug04G0440900
rosa_samantha	Rh5AG069800 Rh5BG066500 Rh5CG077800 Rh5DG065800
rosa_wichuraiana	Rw5G006510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	146
AatII	GACGTC	1 cut(s)	231
AccB1I	GGYRCC	1 cut(s)	1630
AccBSI	CCGCTC	1 cut(s)	222
AccII	CGCG	3 cut(s)	46, 95, 138
AciI	CCGC	6 cut(s)	46, 93, 136, 220, 722, 1465
AclWI	GGATC	2 cut(s)	408, 655
AcsI	RAATTY	4 cut(s)	288, 568, 626, 1588
AcuI	CTGAAG	1 cut(s)	654
AcyI	GRCGYC	1 cut(s)	228
AdeI	CACNNNGTG	1 cut(s)	96
AfaI	GTAC	3 cut(s)	65, 1337, 1546
AfiI	CCNNNNNNNGG	7 cut(s)	189, 732, 1016, 1139, 1259, 1260, 1744
AflIII	ACRYGT	2 cut(s)	174, 1312
AgeI	ACCGGT	2 cut(s)	955, 1132
AjiI	CACGTC	1 cut(s)	998
AjnI	CCWGG	5 cut(s)	151, 212, 698, 1382, 1690
AleI	CACNNNNGTG	1 cut(s)	590
AluBI	AGCT	7 cut(s)	28, 118, 350, 362, 617, 1093, 1720
AluI	AGCT	7 cut(s)	28, 118, 350, 362, 617, 1093, 1720
Alw26I	GTCTC	3 cut(s)	11, 279, 1040
AlwI	GGATC	2 cut(s)	408, 655
AoxI	GGCC	8 cut(s)	723, 1084, 1262, 1344, 1380, 1398, 1610, 1735
ApaI	GGGCCC	1 cut(s)	1088
ApeKI	GCWGC	4 cut(s)	28, 330, 347, 473
ApoI	RAATTY	4 cut(s)	288, 568, 626, 1588
AsiGI	ACCGGT	2 cut(s)	955, 1132
AspLEI	GCGC	2 cut(s)	210, 1044
AsuC2I	CCSGG	2 cut(s)	61, 130
AsuHPI	GGTGA	9 cut(s)	82, 535, 837, 1031, 1066, 1147, 1699, 1759, 1765
AsuII	TTCGAA	1 cut(s)	784
AvaII	GGWCC	6 cut(s)	57, 132, 158, 466, 1484, 1676
AxyI	CCTNAGG	1 cut(s)	1680
BaeGI	GKGCMC	3 cut(s)	171, 876, 1088
BanI	GGYRCC	1 cut(s)	1630
BanII	GRGCYC	3 cut(s)	1009, 1088, 1254
BbvI	GCAGC	4 cut(s)	15, 317, 359, 460
BccI	CCATC	9 cut(s)	194, 368, 426, 653, 755, 1069, 1185, 1197, 1724
BceAI	ACGGC	3 cut(s)	25, 517, 1563
BciT130I	CCWGG	5 cut(s)	153, 214, 700, 1384, 1692
BciVI	GTATCC	1 cut(s)	1066
BclI	TGATCA	2 cut(s)	805, 1527
BcnI	CCSGG	2 cut(s)	61, 130
BcoDI	GTCTC	3 cut(s)	11, 279, 1040
BfmI	CTRYAG	1 cut(s)	1605
BfuI	GTATCC	1 cut(s)	1066
BisI	GCNGC	5 cut(s)	29, 331, 348, 474, 723
BlsI	GCNGC	5 cut(s)	30, 332, 349, 475, 724
Bme1390I	CCNGG	7 cut(s)	61, 130, 153, 214, 700, 1384, 1692
Bme18I	GGWCC	6 cut(s)	57, 132, 158, 466, 1484, 1676
BmgBI	CACGTC	1 cut(s)	998
BmrFI	CCNGG	7 cut(s)	61, 130, 153, 214, 700, 1384, 1692
BmrI	ACTGGG	2 cut(s)	722, 1322
BmsI	GCATC	4 cut(s)	334, 628, 1373, 1460
BmuI	ACTGGG	2 cut(s)	722, 1322
BplI	GAGNNNNNCTC	2 cut(s)	107, 139
BpmI	CTGGAG	1 cut(s)	1178
Bpu14I	TTCGAA	1 cut(s)	784
BpuEI	CTTGAG	1 cut(s)	1447
BpuMI	CCSGG	2 cut(s)	61, 130
Bsa29I	ATCGAT	1 cut(s)	860
BsaAI	YACGTR	1 cut(s)	175
BsaBI	GATNNNNATC	1 cut(s)	804
BsaHI	GRCGYC	1 cut(s)	228
BsaI	GGTCTC	2 cut(s)	11, 1040
BsaJI	CCNNGG	7 cut(s)	151, 678, 698, 699, 901, 1395, 1659
BsaWI	WCCGGW	4 cut(s)	955, 1009, 1132, 1709
Bsc4I	CCNNNNNNNGG	7 cut(s)	189, 732, 1016, 1139, 1259, 1260, 1744
Bse118I	RCCGGY	2 cut(s)	955, 1132
Bse1I	ACTGG	6 cut(s)	331, 649, 728, 820, 1126, 1328
Bse21I	CCTNAGG	1 cut(s)	1680
Bse8I	GATNNNNATC	1 cut(s)	804
BseBI	CCWGG	5 cut(s)	153, 214, 700, 1384, 1692
BseCI	ATCGAT	1 cut(s)	860
BseDI	CCNNGG	7 cut(s)	151, 678, 698, 699, 901, 1395, 1659
BseGI	GGATG	2 cut(s)	349, 688
BseJI	GATNNNNATC	1 cut(s)	804
BseLI	CCNNNNNNNGG	7 cut(s)	189, 732, 1016, 1139, 1259, 1260, 1744
BseMII	CTCAG	2 cut(s)	1051, 1671
BseNI	ACTGG	6 cut(s)	331, 649, 728, 820, 1126, 1328
BseSI	GKGCMC	3 cut(s)	171, 876, 1088
BseXI	GCAGC	4 cut(s)	15, 317, 359, 460
BseYI	CCCAGC	1 cut(s)	432
BsgI	GTGCAG	1 cut(s)	614
Bsh1236I	CGCG	3 cut(s)	46, 95, 138
Bsh1285I	CGRYCG	3 cut(s)	50, 54, 1133
BshFI	GGCC	8 cut(s)	725, 1086, 1264, 1346, 1382, 1400, 1612, 1737
BshNI	GGYRCC	1 cut(s)	1630
BshTI	ACCGGT	2 cut(s)	955, 1132
BshVI	ATCGAT	1 cut(s)	860
BsiEI	CGRYCG	3 cut(s)	50, 54, 1133
BsiSI	CCGG	7 cut(s)	60, 85, 130, 956, 1010, 1133, 1710
BslFI	GGGAC	3 cut(s)	134, 144, 479
BslI	CCNNNNNNNGG	7 cut(s)	189, 732, 1016, 1139, 1259, 1260, 1744
BsmAI	GTCTC	3 cut(s)	11, 279, 1040
BsmFI	GGGAC	3 cut(s)	134, 144, 479
BsmI	GAATGC	2 cut(s)	42, 1149
BsnI	GGCC	8 cut(s)	725, 1086, 1264, 1346, 1382, 1400, 1612, 1737
Bso31I	GGTCTC	2 cut(s)	11, 1040
Bsp119I	TTCGAA	1 cut(s)	784
Bsp120I	GGGCCC	1 cut(s)	1084
Bsp1286I	GDGCHC	5 cut(s)	171, 876, 1009, 1088, 1254
Bsp143I	GATC	8 cut(s)	51, 413, 647, 805, 1021, 1237, 1469, 1527
BspACI	CCGC	6 cut(s)	46, 93, 136, 220, 722, 1465
BspANI	GGCC	8 cut(s)	725, 1086, 1264, 1346, 1382, 1400, 1612, 1737
BspCNI	CTCAG	2 cut(s)	1050, 1672
BspDI	ATCGAT	1 cut(s)	860
BspFNI	CGCG	3 cut(s)	46, 95, 138
BspMAI	CTGCAG	1 cut(s)	1609
BspPI	GGATC	2 cut(s)	408, 655
BspT104I	TTCGAA	1 cut(s)	784
BspT107I	GGYRCC	1 cut(s)	1630
BspTNI	GGTCTC	2 cut(s)	11, 1040
BsrBI	CCGCTC	1 cut(s)	222
BsrFI	RCCGGY	2 cut(s)	955, 1132
BsrI	ACTGG	6 cut(s)	331, 649, 728, 820, 1126, 1328
BssAI	RCCGGY	2 cut(s)	955, 1132
BssECI	CCNNGG	7 cut(s)	151, 678, 698, 699, 901, 1395, 1659
BssMI	GATC	8 cut(s)	51, 413, 647, 805, 1021, 1237, 1469, 1527
BssNI	GRCGYC	1 cut(s)	228
BssT1I	CCWWGG	4 cut(s)	678, 901, 1395, 1659
Bst2UI	CCWGG	5 cut(s)	153, 214, 700, 1384, 1692
Bst4CI	ACNGT	6 cut(s)	745, 775, 835, 843, 1244, 1447
BstACI	GRCGYC	1 cut(s)	228
BstBAI	YACGTR	1 cut(s)	175
BstBI	TTCGAA	1 cut(s)	784
BstC8I	GCNNGC	3 cut(s)	97, 206, 335
BstDEI	CTNAG	4 cut(s)	917, 941, 1037, 1680
BstEII	GGTNACC	1 cut(s)	1705
BstENI	CCTNNNNNAGG	1 cut(s)	187
BstF5I	GGATG	2 cut(s)	349, 688
BstFNI	CGCG	3 cut(s)	46, 95, 138
BstHHI	GCGC	2 cut(s)	210, 1044
BstKTI	GATC	8 cut(s)	54, 416, 650, 808, 1024, 1240, 1472, 1530
BstMAI	GTCTC	3 cut(s)	11, 279, 1040
BstMBI	GATC	8 cut(s)	51, 413, 647, 805, 1021, 1237, 1469, 1527
BstMCI	CGRYCG	3 cut(s)	50, 54, 1133
BstMWI	GCNNNNNNNGC	4 cut(s)	37, 115, 201, 479
BstNI	CCWGG	5 cut(s)	153, 214, 700, 1384, 1692
BstNSI	RCATGY	2 cut(s)	440, 1316
BstPI	GGTNACC	1 cut(s)	1705
BstSCI	CCNGG	7 cut(s)	59, 128, 151, 212, 698, 1382, 1690
BstSFI	CTRYAG	1 cut(s)	1605
BstSLI	GKGCMC	3 cut(s)	171, 876, 1088
BstUI	CGCG	3 cut(s)	46, 95, 138
BstV1I	GCAGC	4 cut(s)	15, 317, 359, 460
BstX2I	RGATCY	1 cut(s)	413
BstXI	CCANNNNNNTGG	3 cut(s)	908, 1002, 1698
BstYI	RGATCY	1 cut(s)	413
Bsu15I	ATCGAT	1 cut(s)	860
Bsu36I	CCTNAGG	1 cut(s)	1680
BsuI	GTATCC	1 cut(s)	1066
BsuRI	GGCC	8 cut(s)	725, 1086, 1264, 1346, 1382, 1400, 1612, 1737
BsuTUI	ATCGAT	1 cut(s)	860
BtrI	CACGTC	1 cut(s)	998
BtsCI	GGATG	2 cut(s)	349, 688
BtsI	GCAGTG	2 cut(s)	933, 1320
BtsIMutI	CAGTG	4 cut(s)	324, 827, 933, 1320
Cac8I	GCNNGC	3 cut(s)	97, 206, 335
CfoI	GCGC	2 cut(s)	210, 1044
Cfr10I	RCCGGY	2 cut(s)	955, 1132
ClaI	ATCGAT	1 cut(s)	860
CsiI	ACCWGGT	1 cut(s)	212
Csp6I	GTAC	3 cut(s)	64, 1336, 1545
CspAI	ACCGGT	2 cut(s)	955, 1132
CspCI	CAANNNNNGTGG	2 cut(s)	1560, 1595
CviAII	CATG	5 cut(s)	437, 462, 553, 923, 1313
CviQI	GTAC	3 cut(s)	64, 1336, 1545
DdeI	CTNAG	4 cut(s)	917, 941, 1037, 1680
DpnI	GATC	8 cut(s)	53, 415, 649, 807, 1023, 1239, 1471, 1529
DpnII	GATC	8 cut(s)	51, 413, 647, 805, 1021, 1237, 1469, 1527
DraI	TTTAAA	1 cut(s)	1110
DraIII	CACNNNGTG	1 cut(s)	96
DrdI	GACNNNNNNGTC	1 cut(s)	146
DseDI	GACNNNNNNGTC	1 cut(s)	146
Eco130I	CCWWGG	4 cut(s)	678, 901, 1395, 1659
Eco147I	AGGCCT	1 cut(s)	1382
Eco24I	GRGCYC	3 cut(s)	1009, 1088, 1254
Eco31I	GGTCTC	2 cut(s)	11, 1040
Eco32I	GATATC	1 cut(s)	82
Eco47I	GGWCC	6 cut(s)	57, 132, 158, 466, 1484, 1676
Eco57I	CTGAAG	1 cut(s)	654
Eco81I	CCTNAGG	1 cut(s)	1680
Eco91I	GGTNACC	1 cut(s)	1705
EcoNI	CCTNNNNNAGG	1 cut(s)	187
EcoO109I	RGGNCCY	4 cut(s)	466, 1344, 1484, 1676
EcoO65I	GGTNACC	1 cut(s)	1705
EcoRI	GAATTC	1 cut(s)	1588
EcoRII	CCWGG	5 cut(s)	151, 212, 698, 1382, 1690
EcoRV	GATATC	1 cut(s)	82
EcoT14I	CCWWGG	4 cut(s)	678, 901, 1395, 1659
EcoT38I	GRGCYC	3 cut(s)	1009, 1088, 1254
ErhI	CCWWGG	4 cut(s)	678, 901, 1395, 1659
FaeI	CATG	5 cut(s)	440, 465, 556, 926, 1316
FaqI	GGGAC	3 cut(s)	134, 144, 479
FatI	CATG	5 cut(s)	436, 461, 552, 922, 1312
FauI	CCCGC	1 cut(s)	143
FbaI	TGATCA	2 cut(s)	805, 1527
Fnu4HI	GCNGC	5 cut(s)	29, 331, 348, 474, 723
FokI	GGATG	2 cut(s)	356, 695
FriOI	GRGCYC	3 cut(s)	1009, 1088, 1254
Fsp4HI	GCNGC	5 cut(s)	29, 331, 348, 474, 723
GlaI	GCGC	2 cut(s)	209, 1043
GluI	GCNGC	5 cut(s)	29, 331, 348, 474, 723
GsaI	CCCAGC	1 cut(s)	436
GsuI	CTGGAG	1 cut(s)	1178
HaeIII	GGCC	8 cut(s)	725, 1086, 1264, 1346, 1382, 1400, 1612, 1737
HapII	CCGG	7 cut(s)	60, 85, 130, 956, 1010, 1133, 1710
HhaI	GCGC	2 cut(s)	210, 1044
Hin1I	GRCGYC	1 cut(s)	228
Hin1II	CATG	5 cut(s)	440, 465, 556, 926, 1316
Hin6I	GCGC	2 cut(s)	208, 1042
HinP1I	GCGC	2 cut(s)	208, 1042
HincII	GTYRAC	2 cut(s)	1102, 1770
HindII	GTYRAC	2 cut(s)	1102, 1770
HindIII	AAGCTT	1 cut(s)	1718
HpaI	GTTAAC	1 cut(s)	1102
HpaII	CCGG	7 cut(s)	60, 85, 130, 956, 1010, 1133, 1710
HphI	GGTGA	9 cut(s)	82, 535, 837, 1031, 1066, 1147, 1699, 1759, 1765
Hpy166II	GTNNAC	5 cut(s)	180, 550, 1102, 1600, 1770
Hpy188I	TCNGA	8 cut(s)	226, 246, 253, 354, 634, 652, 658, 1474
Hpy188III	TCNNGA	7 cut(s)	669, 1157, 1214, 1426, 1500, 1509, 1679
Hpy8I	GTNNAC	5 cut(s)	180, 550, 1102, 1600, 1770
Hpy99I	CGWCG	6 cut(s)	46, 149, 167, 230, 233, 1648
HpyAV	CCTTC	7 cut(s)	334, 465, 780, 1154, 1372, 1475, 1744
HpyCH4III	ACNGT	6 cut(s)	745, 775, 835, 843, 1244, 1447
HpyCH4IV	ACGT	4 cut(s)	174, 228, 997, 1646
HpyF10VI	GCNNNNNNNGC	4 cut(s)	37, 115, 201, 479
HpyF3I	CTNAG	4 cut(s)	917, 941, 1037, 1680
HpySE526I	ACGT	4 cut(s)	174, 228, 997, 1646
Hsp92I	GRCGYC	1 cut(s)	228
Hsp92II	CATG	5 cut(s)	440, 465, 556, 926, 1316
HspAI	GCGC	2 cut(s)	208, 1042
KflI	GGGWCCC	1 cut(s)	466
Ksp22I	TGATCA	2 cut(s)	805, 1527
KspAI	GTTAAC	1 cut(s)	1102
Kzo9I	GATC	8 cut(s)	51, 413, 647, 805, 1021, 1237, 1469, 1527
LmnI	GCTCC	5 cut(s)	227, 355, 1012, 1299, 1712
Lsp1109I	GCAGC	4 cut(s)	15, 317, 359, 460
LweI	GCATC	4 cut(s)	334, 628, 1373, 1460
MabI	ACCWGGT	1 cut(s)	212
MaeII	ACGT	4 cut(s)	174, 228, 997, 1646
MalI	GATC	8 cut(s)	53, 415, 649, 807, 1023, 1239, 1471, 1529
MbiI	CCGCTC	1 cut(s)	222
MboI	GATC	8 cut(s)	51, 413, 647, 805, 1021, 1237, 1469, 1527
MboII	GAAGA	2 cut(s)	647, 1202
MfeI	CAATTG	1 cut(s)	1368
MflI	RGATCY	1 cut(s)	413
MhlI	GDGCHC	5 cut(s)	171, 876, 1009, 1088, 1254
MlyI	GAGTC	5 cut(s)	116, 662, 683, 1352, 1679
MmeI	TCCRAC	4 cut(s)	249, 377, 994, 1249
MseI	TTAA	7 cut(s)	240, 306, 318, 605, 1101, 1109, 1798
MslI	CAYNNNNRTG	7 cut(s)	590, 752, 860, 1000, 1079, 1315, 1406
MspA1I	CMGCKG	1 cut(s)	28
MspI	CCGG	7 cut(s)	60, 85, 130, 956, 1010, 1133, 1710
MspR9I	CCNGG	7 cut(s)	61, 130, 153, 214, 700, 1384, 1692
MunI	CAATTG	1 cut(s)	1368
Mva1269I	GAATGC	2 cut(s)	42, 1149
MvaI	CCWGG	5 cut(s)	153, 214, 700, 1384, 1692
MvnI	CGCG	3 cut(s)	46, 95, 138
MwoI	GCNNNNNNNGC	4 cut(s)	37, 115, 201, 479
NciI	CCSGG	2 cut(s)	61, 130
NdeII	GATC	8 cut(s)	51, 413, 647, 805, 1021, 1237, 1469, 1527
NlaIII	CATG	5 cut(s)	440, 465, 556, 926, 1316
NmeAIII	GCCGAG	1 cut(s)	508
NmuCI	GTSAC	7 cut(s)	745, 843, 1135, 1172, 1372, 1564, 1705
NspI	RCATGY	2 cut(s)	440, 1316
NspV	TTCGAA	1 cut(s)	784
OliI	CACNNNNGTG	1 cut(s)	590
PasI	CCCWGGG	1 cut(s)	699
PceI	AGGCCT	1 cut(s)	1382
PciI	ACATGT	1 cut(s)	1312
PcsI	WCGNNNNNNNCGW	1 cut(s)	171
PctI	GAATGC	2 cut(s)	42, 1149
PfeI	GAWTC	5 cut(s)	931, 1512, 1555, 1740, 1787
PflFI	GACNNNGTC	1 cut(s)	143
PinAI	ACCGGT	2 cut(s)	955, 1132
PkrI	GCNGC	5 cut(s)	30, 332, 349, 475, 724
Ple19I	CGATCG	1 cut(s)	54
PleI	GAGTC	5 cut(s)	116, 661, 683, 1352, 1679
PpsI	GAGTC	5 cut(s)	116, 661, 683, 1352, 1679
Ppu21I	YACGTR	1 cut(s)	175
PpuMI	RGGWCCY	3 cut(s)	466, 1484, 1676
PscI	ACATGT	1 cut(s)	1312
Psp5II	RGGWCCY	3 cut(s)	466, 1484, 1676
Psp6I	CCWGG	5 cut(s)	151, 212, 698, 1382, 1690
PspEI	GGTNACC	1 cut(s)	1705
PspFI	CCCAGC	1 cut(s)	432
PspGI	CCWGG	5 cut(s)	151, 212, 698, 1382, 1690
PspOMI	GGGCCC	1 cut(s)	1084
PspPPI	RGGWCCY	3 cut(s)	466, 1484, 1676
PstI	CTGCAG	1 cut(s)	1609
PsuI	RGATCY	1 cut(s)	413
PsyI	GACNNNGTC	1 cut(s)	143
PvuI	CGATCG	1 cut(s)	54
PvuII	CAGCTG	1 cut(s)	28
RsaI	GTAC	3 cut(s)	65, 1337, 1546
RsaNI	GTAC	3 cut(s)	64, 1336, 1545
RseI	CAYNNNNRTG	7 cut(s)	590, 752, 860, 1000, 1079, 1315, 1406
SaqAI	TTAA	7 cut(s)	240, 306, 318, 605, 1101, 1109, 1798
SatI	GCNGC	5 cut(s)	29, 331, 348, 474, 723
Sau3AI	GATC	8 cut(s)	51, 413, 647, 805, 1021, 1237, 1469, 1527
SbfI	CCTGCAGG	1 cut(s)	1609
SchI	GAGTC	5 cut(s)	116, 662, 683, 1352, 1679
ScrFI	CCNGG	7 cut(s)	61, 130, 153, 214, 700, 1384, 1692
SdaI	CCTGCAGG	1 cut(s)	1609
SduI	GDGCHC	5 cut(s)	171, 876, 1009, 1088, 1254
SexAI	ACCWGGT	1 cut(s)	212
SfaNI	GCATC	4 cut(s)	334, 628, 1373, 1460
SfcI	CTRYAG	1 cut(s)	1605
SfuI	TTCGAA	1 cut(s)	784
SinI	GGWCC	6 cut(s)	57, 132, 158, 466, 1484, 1676
SmiMI	CAYNNNNRTG	7 cut(s)	590, 752, 860, 1000, 1079, 1315, 1406
SmlI	CTYRAG	1 cut(s)	1426
SmoI	CTYRAG	1 cut(s)	1426
Sse8387I	CCTGCAGG	1 cut(s)	1609
SseBI	AGGCCT	1 cut(s)	1382
SsiI	CCGC	6 cut(s)	46, 93, 136, 220, 722, 1465
StuI	AGGCCT	1 cut(s)	1382
StyD4I	CCNGG	7 cut(s)	59, 128, 151, 212, 698, 1382, 1690
StyI	CCWWGG	4 cut(s)	678, 901, 1395, 1659
TaaI	ACNGT	6 cut(s)	745, 775, 835, 843, 1244, 1447
TaiI	ACGT	4 cut(s)	177, 231, 1000, 1649
TaqI	TCGA	9 cut(s)	14, 50, 114, 125, 784, 860, 1129, 1490, 1501
TatI	WGTACW	1 cut(s)	1335
TauI	GCSGC	1 cut(s)	725
TfiI	GAWTC	5 cut(s)	931, 1512, 1555, 1740, 1787
Tru1I	TTAA	7 cut(s)	240, 306, 318, 605, 1101, 1109, 1798
Tru9I	TTAA	7 cut(s)	240, 306, 318, 605, 1101, 1109, 1798
TscAI	CASTG	4 cut(s)	331, 827, 933, 1327
TseFI	GTSAC	7 cut(s)	745, 843, 1135, 1172, 1372, 1564, 1705
TseI	GCWGC	4 cut(s)	28, 330, 347, 473
Tsp45I	GTSAC	7 cut(s)	745, 843, 1135, 1172, 1372, 1564, 1705
TspDTI	ATGAA	5 cut(s)	478, 569, 770, 1504, 1752
TspGWI	ACGGA	1 cut(s)	282
TspRI	CASTG	4 cut(s)	331, 827, 933, 1327
Tth111I	GACNNNGTC	1 cut(s)	143
VpaK11BI	GGWCC	6 cut(s)	57, 132, 158, 466, 1484, 1676
XagI	CCTNNNNNAGG	1 cut(s)	187
XapI	RAATTY	4 cut(s)	288, 568, 626, 1588
XceI	RCATGY	2 cut(s)	440, 1316
ZraI	GACGTC	1 cut(s)	229

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.