Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0010968

Physical Location & Seq

Genomic Coordinates Reverse (-)

44129343 .. 44132550

Gene Structure 3208 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1311300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1812 bp

ATGGCTCTCAATGCGCCTCGCTCCGACCCCCAATCCGCCGCCCAGAAGGCCGTCGCGGTGATCGGGTCCGGGTACGATCTCTGCAACGACATCCGGCTCACCGCGTGCAAGCCCATGCTCGTCGACCTTGACCCGACCCGGACCCGCGACCTCTTCGTCCCTGGCGGGCCCGTCGTGCGCGGCGTCTCCACCTCCATCAGGTGCGATAAAGGCGAACGCACCAGGTTGCGCTCCGACGTCGTTTCGTTTAATCAGATGTCAGAGCAATGCAACAAGGAACTGTCTCTAACGGGCAAAATTCCGTCTGGTCTGTTTAATGCTATGTTTGGATTCACTGGCTGCTGGCAGAAGGATGCAGCTCCCGTAAAAGCTCTTGCTTGTGATGGTTGGTTGATAACTCTGTATGACATTGAGCTAGCGAGATCCCATGTAAAGCTATCCGAGCATGTGAGACAGGAAGTGCCTTCTACATGGGACCCTGCTGCCCTTGCTGCGTTCATTGAAAAATACGGTACTCATATTGTTGTAGGGGTGAGGATGGGTGGAAAGGATGTAGTTCACATAAAGCAGTTGCAAAATTCAAATCTTCATCCCACAGAAGTGCAGGAATTGTTAAAGCAATTAGCCGACGAGAGGTTTTCTGAGGATGGAACTGGATCGGAGTCCAATGATTTATCAGGAAAAACGAAGGATGACGATTCTATGCACTGGGATGTTCGTAGAATGTATGCACCCTCAATAAGGCGACCTATCATCACACAAAGGAATGATGGTACAGTTACTGTTTGTGTTCGAAGGGGAGGTGTTGATATTAGTCAAAGTCATAACAAGTGGCTTTCAACCGTATCAGAGTCACCTGATGTCGTATTGATGTCCTTCGTGCCCCTAACCTCTCTGTTGGGTGGCGTCCAAGGAATTGGATTTCTAAGTCATGCAGTGAATCTCTACCTTAGATACAAACCGCCAATAGAGGAACTCCATCAATTTTTGGAATTTCAGTTACCACGTCAATGGGCTCCAGTGTATGGCGATCTCCCTCTCGGTCTCAGGCACAGAAAACAAGTATCCCCATCACTTCAGTTCACCTTTATGGGCCCAAAGCTATACGTTAACACTATGAAGGTTGATACTGGAAATCGACCGGTGACAGGAATGCGTTTGTATCTTGAAGGTAAGAAAAGTGACCATCTGGCAATCCATCTCCAGCACCTTTCTACTCTTCCCAGTACTCTCCAACTCTCAGATGATCACAGTTATGATCCCATTGACGAGCCAGAGGAACGAGGCTACTTTGAACCTGTCAAGTGGAGCATATTTTCACATGTATGCACTGCCCCAGTACAGTACAATGGGGCCTGTATTGATGATTCGGCTTCAATTGTGACAAAGGCCTGGTTTGAAGCCAAGTCTGTCGGAATGAGGAAAGTTTTGTTCCTGAGGCTAGGATTTTCAACAGTGGCGTCTGCAAAACTCCGAAGGTCAGAGTGGGAAGGACCCTCAAGCTCTTCTCGAAAATCAGGATTCATCTCGACCTTGATGAGCACAAGGTTCAGTACTGCATTGAATCCGCCTGTGACGCCCAAAAAAGTGGATTTGAATTCAGCAGTTTACCCCACAGGGCCACCTTTACCAAGCAAGGCACCGAAAATGGCGACGTTTGTAGATACCAATGAAATGTTAAGAGGTCCCGAGGACTCGCCAGGATATTGGGTGGTCACCGGCGCCAAGCTCTGTGTAGAGGGTGGGCGAATCTCAGTGAAAGTGAAGTATTCGTTATTGACAATAATGTCAGAGGATTCTATGATGTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008219 GO:0009626 GO:0009628 GO:0009651 GO:0009987 GO:0010337 GO:0010565 GO:0010817 GO:0012501 GO:0019222 GO:0031323 GO:0032350 GO:0033036 GO:0033037 GO:0033554 GO:0034050 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051716 GO:0052542 GO:0052545 GO:0062012 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MACPF

Protein Analysis

604

Amino Acids

66.76

Weight (kDa)

8.52

Isoelectric Point (pI)

52.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MACPF	PF01823	104 - 316	4.1e-33	MAC/Perforin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28380
fragaria_vesca	FvH4_3g08740
malus_domestica	MD05G1311300.v1.1 MD10G1290300.v1.1
prunus_persica	Prupe.4G051500_v2.0.a1
pyrus_communis	pycom05g28740 pycom10g24250 pycom10g24280
rosa_chinensis	RchiOBHm_Chr5g0009001
rosa_laevigata	RLG00000031624
rosa_multiflora	Rmu_sc0021361.1_g000001
rosa_roxburghii	Rroxscaffold_1G00067010 Rroxscaffold_1G00067650
rosa_rugosa	Rorug04G0440800 Rorug04G0440900
rosa_samantha	Rh5AG069800 Rh5BG066500 Rh5CG077800 Rh5DG065800
rosa_wichuraiana	Rw5G006510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	155, 1786
AatII	GACGTC	1 cut(s)	240
AccB1I	GGYRCC	2 cut(s)	1639, 1721
AccB7I	CCANNNNNTGG	1 cut(s)	1025
AccI	GTMKAC	1 cut(s)	123
AccII	CGCG	4 cut(s)	56, 104, 147, 180
AciI	CCGC	9 cut(s)	36, 39, 56, 102, 145, 165, 180, 962, 1568
AclWI	GGATC	3 cut(s)	417, 664, 1253
AcsI	RAATTY	4 cut(s)	297, 577, 992, 1597
AcuI	CTGAAG	1 cut(s)	1061
AcyI	GRCGYC	6 cut(s)	183, 237, 906, 1460, 1577, 1722
AdeI	CACNNNGTG	1 cut(s)	105
AfaI	GTAC	7 cut(s)	74, 514, 775, 1228, 1341, 1346, 1555
AfiI	CCNNNNNNNGG	5 cut(s)	198, 633, 741, 1025, 1148
AflIII	ACRYGT	1 cut(s)	1321
AgeI	ACCGGT	1 cut(s)	1141
AjiI	CACGTC	1 cut(s)	1007
AjnI	CCWGG	4 cut(s)	160, 221, 1391, 1699
AleI	CACNNNNGTG	1 cut(s)	599
AluBI	AGCT	7 cut(s)	359, 371, 415, 436, 1102, 1503, 1729
AluI	AGCT	7 cut(s)	359, 371, 415, 436, 1102, 1503, 1729
Alw21I	GWGCWC	1 cut(s)	1544
Alw26I	GTCTC	4 cut(s)	190, 288, 445, 1049
AlwI	GGATC	3 cut(s)	417, 664, 1253
AlwNI	CAGNNNCTG	1 cut(s)	782
Ama87I	CYCGRG	1 cut(s)	1688
AoxI	GGCC	6 cut(s)	48, 167, 1093, 1353, 1389, 1619
ApaI	GGGCCC	2 cut(s)	171, 1097
ApeKI	GCWGC	4 cut(s)	339, 356, 482, 491
ApoI	RAATTY	4 cut(s)	297, 577, 992, 1597
AsiGI	ACCGGT	1 cut(s)	1141
AspLEI	GCGC	4 cut(s)	16, 180, 231, 1724
AsuC2I	CCSGG	2 cut(s)	70, 139
AsuHPI	GGTGA	7 cut(s)	70, 91, 544, 846, 1075, 1156, 1708
AsuII	TTCGAA	1 cut(s)	793
AsuNHI	GCTAGC	1 cut(s)	415
AvaI	CYCGRG	1 cut(s)	1688
AvaII	GGWCC	5 cut(s)	66, 141, 475, 1493, 1685
AxyI	CCTNAGG	1 cut(s)	1436
BaeGI	GKGCMC	3 cut(s)	171, 885, 1097
BanI	GGYRCC	2 cut(s)	1639, 1721
BanII	GRGCYC	3 cut(s)	171, 1018, 1097
Bbv12I	GWGCWC	1 cut(s)	1544
BbvI	GCAGC	4 cut(s)	326, 368, 469, 478
BccI	CCATC	9 cut(s)	203, 377, 532, 641, 764, 987, 1078, 1194, 1206
BceAI	ACGGC	1 cut(s)	35
BciT130I	CCWGG	4 cut(s)	162, 223, 1393, 1701
BciVI	GTATCC	1 cut(s)	1075
BclI	TGATCA	1 cut(s)	1246
BcnI	CCSGG	2 cut(s)	70, 139
BcoDI	GTCTC	4 cut(s)	190, 288, 445, 1049
BfaI	CTAG	2 cut(s)	416, 1442
BfoI	RGCGCY	1 cut(s)	1725
BfuI	GTATCC	1 cut(s)	1075
BglI	GCCNNNNNGGC	1 cut(s)	47
BisI	GCNGC	6 cut(s)	39, 181, 340, 357, 483, 492
BlsI	GCNGC	6 cut(s)	40, 182, 341, 358, 484, 493
BmcAI	AGTACT	2 cut(s)	1228, 1555
Bme1390I	CCNGG	6 cut(s)	70, 139, 162, 223, 1393, 1701
Bme18I	GGWCC	5 cut(s)	66, 141, 475, 1493, 1685
BmeT110I	CYCGRG	1 cut(s)	1688
BmgBI	CACGTC	1 cut(s)	1007
BmrFI	CCNGG	6 cut(s)	70, 139, 162, 223, 1393, 1701
BmrI	ACTGGG	3 cut(s)	718, 1218, 1331
BmsI	GCATC	1 cut(s)	343
BmtI	GCTAGC	1 cut(s)	419
BmuI	ACTGGG	3 cut(s)	718, 1218, 1331
BpmI	CTGGAG	2 cut(s)	1002, 1187
Bpu14I	TTCGAA	1 cut(s)	793
BpuEI	CTTGAG	1 cut(s)	1483
BpuMI	CCSGG	2 cut(s)	70, 139
BsaHI	GRCGYC	6 cut(s)	183, 237, 906, 1460, 1577, 1722
BsaI	GGTCTC	1 cut(s)	1049
BsaJI	CCNNGG	3 cut(s)	160, 910, 1689
BsaWI	WCCGGW	1 cut(s)	1141
Bsc4I	CCNNNNNNNGG	5 cut(s)	198, 633, 741, 1025, 1148
Bse118I	RCCGGY	2 cut(s)	1141, 1718
Bse1I	ACTGG	7 cut(s)	340, 658, 713, 1019, 1135, 1224, 1337
Bse21I	CCTNAGG	1 cut(s)	1436
Bse3DI	GCAATG	1 cut(s)	272
BseBI	CCWGG	4 cut(s)	162, 223, 1393, 1701
BseDI	CCNNGG	3 cut(s)	160, 910, 1689
BseGI	GGATG	8 cut(s)	90, 358, 543, 556, 589, 652, 697, 718
BseLI	CCNNNNNNNGG	5 cut(s)	198, 633, 741, 1025, 1148
BseMI	GCAATG	1 cut(s)	272
BseMII	CTCAG	5 cut(s)	633, 1060, 1254, 1427, 1767
BseNI	ACTGG	7 cut(s)	340, 658, 713, 1019, 1135, 1224, 1337
BseSI	GKGCMC	3 cut(s)	171, 885, 1097
BseXI	GCAGC	4 cut(s)	326, 368, 469, 478
BsgI	GTGCAG	1 cut(s)	623
Bsh1236I	CGCG	4 cut(s)	56, 104, 147, 180
Bsh1285I	CGRYCG	1 cut(s)	1142
BshFI	GGCC	6 cut(s)	50, 169, 1095, 1355, 1391, 1621
BshNI	GGYRCC	2 cut(s)	1639, 1721
BshTI	ACCGGT	1 cut(s)	1141
BsiEI	CGRYCG	1 cut(s)	1142
BsiHKAI	GWGCWC	1 cut(s)	1544
BsiHKCI	CYCGRG	1 cut(s)	1688
BsiSI	CCGG	5 cut(s)	69, 94, 139, 1142, 1719
BslFI	GGGAC	3 cut(s)	143, 488, 1671
BslI	CCNNNNNNNGG	5 cut(s)	198, 633, 741, 1025, 1148
BsmAI	GTCTC	4 cut(s)	190, 288, 445, 1049
BsmBI	CGTCTC	1 cut(s)	190
BsmFI	GGGAC	3 cut(s)	143, 488, 1671
BsmI	GAATGC	1 cut(s)	1158
BsnI	GGCC	6 cut(s)	50, 169, 1095, 1355, 1391, 1621
Bso31I	GGTCTC	1 cut(s)	1049
BsoBI	CYCGRG	1 cut(s)	1688
Bsp119I	TTCGAA	1 cut(s)	793
Bsp120I	GGGCCC	2 cut(s)	167, 1093
Bsp1286I	GDGCHC	5 cut(s)	171, 885, 1018, 1097, 1544
Bsp143I	GATC	7 cut(s)	60, 76, 422, 656, 1030, 1246, 1258
BspACI	CCGC	9 cut(s)	36, 39, 56, 102, 145, 165, 180, 962, 1568
BspANI	GGCC	6 cut(s)	50, 169, 1095, 1355, 1391, 1621
BspCNI	CTCAG	5 cut(s)	634, 1059, 1253, 1428, 1766
BspFNI	CGCG	4 cut(s)	56, 104, 147, 180
BspOI	GCTAGC	1 cut(s)	419
BspPI	GGATC	3 cut(s)	417, 664, 1253
BspQI	GCTCTTC	1 cut(s)	1510
BspT104I	TTCGAA	1 cut(s)	793
BspT107I	GGYRCC	2 cut(s)	1639, 1721
BspTNI	GGTCTC	1 cut(s)	1049
BsrDI	GCAATG	1 cut(s)	272
BsrFI	RCCGGY	2 cut(s)	1141, 1718
BsrI	ACTGG	7 cut(s)	340, 658, 713, 1019, 1135, 1224, 1337
BssAI	RCCGGY	2 cut(s)	1141, 1718
BssECI	CCNNGG	3 cut(s)	160, 910, 1689
BssMI	GATC	7 cut(s)	60, 76, 422, 656, 1030, 1246, 1258
BssNI	GRCGYC	6 cut(s)	183, 237, 906, 1460, 1577, 1722
BssT1I	CCWWGG	1 cut(s)	910
Bst2UI	CCWGG	4 cut(s)	162, 223, 1393, 1701
Bst4CI	ACNGT	8 cut(s)	282, 512, 778, 784, 844, 1253, 1344, 1456
Bst6I	CTCTTC	3 cut(s)	158, 1224, 1510
BstACI	GRCGYC	6 cut(s)	183, 237, 906, 1460, 1577, 1722
BstBI	TTCGAA	1 cut(s)	793
BstC8I	GCNNGC	5 cut(s)	106, 110, 167, 344, 417
BstDEI	CTNAG	7 cut(s)	642, 926, 950, 1046, 1240, 1436, 1753
BstEII	GGTNACC	1 cut(s)	1714
BstENI	CCTNNNNNAGG	2 cut(s)	196, 739
BstF5I	GGATG	8 cut(s)	90, 358, 543, 556, 589, 652, 697, 718
BstFNI	CGCG	4 cut(s)	56, 104, 147, 180
BstH2I	RGCGCY	1 cut(s)	1725
BstHHI	GCGC	4 cut(s)	16, 180, 231, 1724
BstKTI	GATC	7 cut(s)	63, 79, 425, 659, 1033, 1249, 1261
BstMAI	GTCTC	4 cut(s)	190, 288, 445, 1049
BstMBI	GATC	7 cut(s)	60, 76, 422, 656, 1030, 1246, 1258
BstMCI	CGRYCG	1 cut(s)	1142
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 47, 175, 210, 442, 488, 491, 1576
BstNI	CCWGG	4 cut(s)	162, 223, 1393, 1701
BstNSI	RCATGY	2 cut(s)	449, 1325
BstPI	GGTNACC	1 cut(s)	1714
BstSCI	CCNGG	6 cut(s)	68, 137, 160, 221, 1391, 1699
BstSLI	GKGCMC	3 cut(s)	171, 885, 1097
BstUI	CGCG	4 cut(s)	56, 104, 147, 180
BstV1I	GCAGC	4 cut(s)	326, 368, 469, 478
BstX2I	RGATCY	1 cut(s)	422
BstXI	CCANNNNNNTGG	4 cut(s)	917, 1011, 1588, 1707
BstYI	RGATCY	1 cut(s)	422
Bsu36I	CCTNAGG	1 cut(s)	1436
BsuI	GTATCC	1 cut(s)	1075
BsuRI	GGCC	6 cut(s)	50, 169, 1095, 1355, 1391, 1621
BtrI	CACGTC	1 cut(s)	1007
BtsCI	GGATG	8 cut(s)	90, 358, 543, 556, 589, 652, 697, 718
BtsI	GCAGTG	2 cut(s)	942, 1329
BtsIMutI	CAGTG	7 cut(s)	333, 706, 942, 1026, 1329, 1461, 1761
Cac8I	GCNNGC	5 cut(s)	106, 110, 167, 344, 417
CaiI	CAGNNNCTG	1 cut(s)	782
CfoI	GCGC	4 cut(s)	16, 180, 231, 1724
Cfr10I	RCCGGY	2 cut(s)	1141, 1718
CseI	GACGC	4 cut(s)	172, 895, 1449, 1585
CsiI	ACCWGGT	1 cut(s)	221
Csp6I	GTAC	7 cut(s)	73, 513, 774, 1227, 1340, 1345, 1554
CspAI	ACCGGT	1 cut(s)	1141
CviAII	CATG	6 cut(s)	115, 428, 446, 471, 932, 1322
CviQI	GTAC	7 cut(s)	73, 513, 774, 1227, 1340, 1345, 1554
DdeI	CTNAG	7 cut(s)	642, 926, 950, 1046, 1240, 1436, 1753
DinI	GGCGCC	1 cut(s)	1723
DpnI	GATC	7 cut(s)	62, 78, 424, 658, 1032, 1248, 1260
DpnII	GATC	7 cut(s)	60, 76, 422, 656, 1030, 1246, 1258
DraIII	CACNNNGTG	1 cut(s)	105
DrdI	GACNNNNNNGTC	2 cut(s)	155, 1786
DseDI	GACNNNNNNGTC	2 cut(s)	155, 1786
Eam1104I	CTCTTC	3 cut(s)	158, 1224, 1510
EarI	CTCTTC	3 cut(s)	158, 1224, 1510
EciI	GGCGGA	2 cut(s)	25, 1557
Eco130I	CCWWGG	1 cut(s)	910
Eco147I	AGGCCT	1 cut(s)	1391
Eco24I	GRGCYC	3 cut(s)	171, 1018, 1097
Eco31I	GGTCTC	1 cut(s)	1049
Eco47I	GGWCC	5 cut(s)	66, 141, 475, 1493, 1685
Eco57I	CTGAAG	1 cut(s)	1061
Eco81I	CCTNAGG	1 cut(s)	1436
Eco88I	CYCGRG	1 cut(s)	1688
Eco91I	GGTNACC	1 cut(s)	1714
EcoNI	CCTNNNNNAGG	2 cut(s)	196, 739
EcoO109I	RGGNCCY	4 cut(s)	475, 1353, 1493, 1685
EcoO65I	GGTNACC	1 cut(s)	1714
EcoRI	GAATTC	1 cut(s)	1597
EcoRII	CCWGG	4 cut(s)	160, 221, 1391, 1699
EcoT14I	CCWWGG	1 cut(s)	910
EcoT38I	GRGCYC	3 cut(s)	171, 1018, 1097
EgeI	GGCGCC	1 cut(s)	1723
EheI	GGCGCC	1 cut(s)	1723
ErhI	CCWWGG	1 cut(s)	910
Esp3I	CGTCTC	1 cut(s)	190
FaeI	CATG	6 cut(s)	118, 431, 449, 474, 935, 1325
FaqI	GGGAC	3 cut(s)	143, 488, 1671
FatI	CATG	6 cut(s)	114, 427, 445, 470, 931, 1321
FauI	CCCGC	2 cut(s)	152, 158
FbaI	TGATCA	1 cut(s)	1246
FblI	GTMKAC	1 cut(s)	123
Fnu4HI	GCNGC	6 cut(s)	39, 181, 340, 357, 483, 492
FokI	GGATG	8 cut(s)	77, 365, 550, 563, 576, 659, 704, 725
FriOI	GRGCYC	3 cut(s)	171, 1018, 1097
Fsp4HI	GCNGC	6 cut(s)	39, 181, 340, 357, 483, 492
FspBI	CTAG	2 cut(s)	416, 1442
GlaI	GCGC	4 cut(s)	15, 179, 230, 1723
GluI	GCNGC	6 cut(s)	39, 181, 340, 357, 483, 492
GsuI	CTGGAG	2 cut(s)	1002, 1187
HaeII	RGCGCY	1 cut(s)	1725
HaeIII	GGCC	6 cut(s)	50, 169, 1095, 1355, 1391, 1621
HapII	CCGG	5 cut(s)	69, 94, 139, 1142, 1719
HgaI	GACGC	4 cut(s)	172, 895, 1449, 1585
HhaI	GCGC	4 cut(s)	16, 180, 231, 1724
Hin1I	GRCGYC	6 cut(s)	183, 237, 906, 1460, 1577, 1722
Hin1II	CATG	6 cut(s)	118, 431, 449, 474, 935, 1325
Hin6I	GCGC	4 cut(s)	14, 178, 229, 1722
HinP1I	GCGC	4 cut(s)	14, 178, 229, 1722
HincII	GTYRAC	2 cut(s)	124, 1111
HindII	GTYRAC	2 cut(s)	124, 1111
HpaI	GTTAAC	1 cut(s)	1111
HpaII	CCGG	5 cut(s)	69, 94, 139, 1142, 1719
HphI	GGTGA	7 cut(s)	70, 91, 544, 846, 1075, 1156, 1708
Hpy166II	GTNNAC	5 cut(s)	124, 559, 1083, 1111, 1609
Hpy188III	TCNNGA	7 cut(s)	678, 1166, 1435, 1509, 1518, 1528, 1688
Hpy8I	GTNNAC	5 cut(s)	124, 559, 1083, 1111, 1609
Hpy99I	CGWCG	7 cut(s)	56, 125, 176, 239, 242, 632, 1657
HpyCH4III	ACNGT	8 cut(s)	282, 512, 778, 784, 844, 1253, 1344, 1456
HpyCH4IV	ACGT	4 cut(s)	237, 1006, 1107, 1655
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 47, 175, 210, 442, 488, 491, 1576
HpyF3I	CTNAG	7 cut(s)	642, 926, 950, 1046, 1240, 1436, 1753
HpySE526I	ACGT	4 cut(s)	237, 1006, 1107, 1655
Hsp92I	GRCGYC	6 cut(s)	183, 237, 906, 1460, 1577, 1722
Hsp92II	CATG	6 cut(s)	118, 431, 449, 474, 935, 1325
HspAI	GCGC	4 cut(s)	14, 178, 229, 1722
KasI	GGCGCC	1 cut(s)	1721
KflI	GGGWCCC	1 cut(s)	475
Ksp22I	TGATCA	1 cut(s)	1246
KspAI	GTTAAC	1 cut(s)	1111
Kzo9I	GATC	7 cut(s)	60, 76, 422, 656, 1030, 1246, 1258
LguI	GCTCTTC	1 cut(s)	1510
LmnI	GCTCC	5 cut(s)	26, 236, 364, 1021, 1308
Lsp1109I	GCAGC	4 cut(s)	326, 368, 469, 478
LweI	GCATC	1 cut(s)	343
MabI	ACCWGGT	1 cut(s)	221
MaeI	CTAG	2 cut(s)	416, 1442
MaeII	ACGT	4 cut(s)	237, 1006, 1107, 1655
MaeIII	GTNAC	8 cut(s)	778, 852, 999, 1144, 1181, 1381, 1573, 1714
MalI	GATC	7 cut(s)	62, 78, 424, 658, 1032, 1248, 1260
MboI	GATC	7 cut(s)	60, 76, 422, 656, 1030, 1246, 1258
MboII	GAAGA	4 cut(s)	145, 578, 1211, 1497
MfeI	CAATTG	1 cut(s)	1377
MflI	RGATCY	1 cut(s)	422
MhlI	GDGCHC	5 cut(s)	171, 885, 1018, 1097, 1544
MluCI	AATT	9 cut(s)	297, 577, 608, 620, 915, 983, 992, 1377, 1597
Mly113I	GGCGCC	1 cut(s)	1722
MlyI	GAGTC	3 cut(s)	671, 860, 1688
MmeI	TCCRAC	4 cut(s)	48, 258, 1258, 1393
MseI	TTAA	5 cut(s)	249, 315, 614, 1110, 1679
MslI	CAYNNNNRTG	6 cut(s)	599, 711, 1009, 1088, 1254, 1324
MspI	CCGG	5 cut(s)	69, 94, 139, 1142, 1719
MspR9I	CCNGG	6 cut(s)	70, 139, 162, 223, 1393, 1701
MunI	CAATTG	1 cut(s)	1377
Mva1269I	GAATGC	1 cut(s)	1158
MvaI	CCWGG	4 cut(s)	162, 223, 1393, 1701
MvnI	CGCG	4 cut(s)	56, 104, 147, 180
MwoI	GCNNNNNNNGC	8 cut(s)	11, 47, 175, 210, 442, 488, 491, 1576
NarI	GGCGCC	1 cut(s)	1722
NciI	CCSGG	2 cut(s)	70, 139
NdeII	GATC	7 cut(s)	60, 76, 422, 656, 1030, 1246, 1258
NheI	GCTAGC	1 cut(s)	415
NlaIII	CATG	6 cut(s)	118, 431, 449, 474, 935, 1325
NmuCI	GTSAC	6 cut(s)	852, 1144, 1181, 1381, 1573, 1714
NspI	RCATGY	2 cut(s)	449, 1325
NspV	TTCGAA	1 cut(s)	793
OliI	CACNNNNGTG	1 cut(s)	599
PceI	AGGCCT	1 cut(s)	1391
PciI	ACATGT	1 cut(s)	1321
PciSI	GCTCTTC	1 cut(s)	1510
PcsI	WCGNNNNNNNCGW	1 cut(s)	180
PctI	GAATGC	1 cut(s)	1158
PfeI	GAWTC	8 cut(s)	330, 698, 940, 1367, 1521, 1564, 1749, 1796
PflMI	CCANNNNNTGG	1 cut(s)	1025
PinAI	ACCGGT	1 cut(s)	1141
PkrI	GCNGC	6 cut(s)	40, 182, 341, 358, 484, 493
PleI	GAGTC	3 cut(s)	670, 859, 1688
PluTI	GGCGCC	1 cut(s)	1725
PpsI	GAGTC	3 cut(s)	670, 859, 1688
PpuMI	RGGWCCY	3 cut(s)	475, 1493, 1685
PscI	ACATGT	1 cut(s)	1321
Psp5II	RGGWCCY	3 cut(s)	475, 1493, 1685
Psp6I	CCWGG	4 cut(s)	160, 221, 1391, 1699
PspEI	GGTNACC	1 cut(s)	1714
PspGI	CCWGG	4 cut(s)	160, 221, 1391, 1699
PspOMI	GGGCCC	2 cut(s)	167, 1093
PspPPI	RGGWCCY	3 cut(s)	475, 1493, 1685
PstNI	CAGNNNCTG	1 cut(s)	782
PsuI	RGATCY	1 cut(s)	422
RsaI	GTAC	7 cut(s)	74, 514, 775, 1228, 1341, 1346, 1555
RsaNI	GTAC	7 cut(s)	73, 513, 774, 1227, 1340, 1345, 1554
RseI	CAYNNNNRTG	6 cut(s)	599, 711, 1009, 1088, 1254, 1324
SalI	GTCGAC	1 cut(s)	122
SapI	GCTCTTC	1 cut(s)	1510
SaqAI	TTAA	5 cut(s)	249, 315, 614, 1110, 1679
SatI	GCNGC	6 cut(s)	39, 181, 340, 357, 483, 492
Sau3AI	GATC	7 cut(s)	60, 76, 422, 656, 1030, 1246, 1258
ScaI	AGTACT	2 cut(s)	1228, 1555
SchI	GAGTC	3 cut(s)	671, 860, 1688
ScrFI	CCNGG	6 cut(s)	70, 139, 162, 223, 1393, 1701
SduI	GDGCHC	5 cut(s)	171, 885, 1018, 1097, 1544
SexAI	ACCWGGT	1 cut(s)	221
SfaNI	GCATC	1 cut(s)	343
SfoI	GGCGCC	1 cut(s)	1723
SfuI	TTCGAA	1 cut(s)	793
SgrAI	CRCCGGYG	1 cut(s)	1718
SinI	GGWCC	5 cut(s)	66, 141, 475, 1493, 1685
SmiMI	CAYNNNNRTG	6 cut(s)	599, 711, 1009, 1088, 1254, 1324
SmlI	CTYRAG	1 cut(s)	1498
SmoI	CTYRAG	1 cut(s)	1498
Sse9I	AATT	9 cut(s)	297, 577, 608, 620, 915, 983, 992, 1377, 1597
SseBI	AGGCCT	1 cut(s)	1391
SsiI	CCGC	9 cut(s)	36, 39, 56, 102, 145, 165, 180, 962, 1568
SspDI	GGCGCC	1 cut(s)	1721
SspMI	CTAG	2 cut(s)	416, 1442
StuI	AGGCCT	1 cut(s)	1391
StyD4I	CCNGG	6 cut(s)	68, 137, 160, 221, 1391, 1699
StyI	CCWWGG	1 cut(s)	910
TaaI	ACNGT	8 cut(s)	282, 512, 778, 784, 844, 1253, 1344, 1456
TaiI	ACGT	4 cut(s)	240, 1009, 1110, 1658
TaqI	TCGA	5 cut(s)	123, 793, 1138, 1510, 1529
TaqII	GACCGA	1 cut(s)	1031
TasI	AATT	9 cut(s)	297, 577, 608, 620, 915, 983, 992, 1377, 1597
TatI	WGTACW	4 cut(s)	1226, 1339, 1344, 1553
TauI	GCSGC	2 cut(s)	41, 183
TfiI	GAWTC	8 cut(s)	330, 698, 940, 1367, 1521, 1564, 1749, 1796
Tru1I	TTAA	5 cut(s)	249, 315, 614, 1110, 1679
Tru9I	TTAA	5 cut(s)	249, 315, 614, 1110, 1679
TscAI	CASTG	7 cut(s)	340, 713, 942, 1026, 1336, 1461, 1761
TseFI	GTSAC	6 cut(s)	852, 1144, 1181, 1381, 1573, 1714
TseI	GCWGC	4 cut(s)	339, 356, 482, 491
Tsp45I	GTSAC	6 cut(s)	852, 1144, 1181, 1381, 1573, 1714
TspDTI	ATGAA	5 cut(s)	487, 578, 1133, 1513, 1686
TspGWI	ACGGA	1 cut(s)	291
TspRI	CASTG	7 cut(s)	340, 713, 942, 1026, 1336, 1461, 1761
Van91I	CCANNNNNTGG	1 cut(s)	1025
VpaK11BI	GGWCC	5 cut(s)	66, 141, 475, 1493, 1685
XagI	CCTNNNNNAGG	2 cut(s)	196, 739
XapI	RAATTY	4 cut(s)	297, 577, 992, 1597
XceI	RCATGY	2 cut(s)	449, 1325
XmiI	GTMKAC	1 cut(s)	123
XspI	CTAG	2 cut(s)	416, 1442
ZraI	GACGTC	1 cut(s)	238
ZrmI	AGTACT	2 cut(s)	1228, 1555

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.