Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010968

Physical Location & Seq

Genomic Coordinates Reverse (-)

37877582 .. 37881172

Gene Structure 3591 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1290300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1809 bp

ATGGCTCTCAATGGGTCTCGCTCCGACCCCCAATCAGCTGCCGAGAAGGCCGTCGCGGTGATCGGGTCCGGGTACAATCTCTGCAACGATATCCGGCTCACCGCGTGCAAGTCCATGCTCATCGAGCTGGACTCGACCCGGACCCGCGACCTCGTCGTCCCTGGTGGTCCCGTCGTGCCCGACGTCTCCACCTCCATCAGGTGCGATAAAGGCGAGCGCACCAGGTTCCGCTCCGACGTCGTTTCGTTTAATCAGATGTCAGAGCAATACAACAAGGAAGTGTCCCTATCGGGCAAAATTCCGTCCGGTCTGTTTAATGCTATGTTTAACTTCACTGGCTGCTGGCAGAAGGATGCAGCTCCAACAAAAGCTCTTGCTTTTGATGGTTGGTTGATAACTCTGTATAACATTGAGTTAGCAAGATGCCATCTAAAACTATCCCAGCATGTGATACAAGAAGTGCCTTCTACATGGGACCCTGCTGCCCTTGCTGAGTTCATTGACAAATACGGTACTCATATTGTTGTTGGGGTGAAAATGGGTGGAAAGGATATAGTTCACATGAAGCAGTTACAAAATTCAAATCTTCATCCCACAGAAGTGCAGAAATTGTTAAAGCAATTAGCCGATGAGATGTTTTCTGAAAATGCAACTGGATCAGAGTCCGATGGTTTATCAGGAAAAACGAAGGATGATGACTCTATGCCCCGGGAGGTTCGTAGAACGCATGCGGCCCCAATAAGGCCACCTACTGTCAGACAAATAAATGATGGTATAATTACTGTCTGTGTTCGAAGGGGAGGTGTTGATATTGATCAAAGTCATAACAAGTGGCTTTCAACCGTATCACAGTCACCTAATGTCATATCGATGTCCTTCGTGCCCATAACCTCTCTGTTGGGTGGTGTCCAAGGAATTGGATTTCTAAGCCATGCAGTGAATCTCTACCTTAGATACAAACCACCAATAGAGGAACTCCAACTATTTTTGGAATATCAGTTACCACGTCAATGGGCTCCGGTGTATGGTGATCTTCCTCTCGGTCTCAGGCGCAGAAAACAAGTATCCCCATCACTGCAATTCACCTTTATGGGCCCAAAGCTATATGTTAACACTTTGAAGGTTGATACTGGAAATCGACCGGTGACAGGAATGCGTTTGTATCTTGAAGGTAAGAAAAGTGACCATCTGGCAATCCATCTCCAGCACCTTGCAACTCTTCCCGATACTCTCCAACTCTCGTACGATCACAGTTACGAGCCCATTCACAAGCCAGAGGAACGAGGCTACTTTGAACCTGTCAAGTGGAGCATATTTTCACATGTATGCACTGCCCCAGTAGAGTACAACGGGGCCTGTATTGATGACTCTGCATCAATTGTGACAAAGGCCTGGTTTGAAGCCAAGGCCATCGGAATGAGGAAGGTTTTGTTCTTGAGGCTTGGATTTTCAACGGTGGCATCTGCAAAAATCCGCAGATCAGAGTGGGAAGGACCCTCGACGACTTCTCGAAAATCAGGATTCATCTCAACCTTGATCAGCACAAGGTTTAGTACGGCATTGAATCCACCTGTGACACCAGCAAAAGTGGATTTGAATTCAGCAGTTTACCCTGCAGGGCCACCTTTACCAAGCAAGGCACCAAAAATGGCGACGTTTGTAGATACCAAGGAAATGTTGAGAGGTCCTGAGGACTCGCCAGGATATTGGGTGGTCACCGGAGCCAAGCTTTGTGTAGAGGGAGGCCGAATCTCGGTGAAGGTGAAGTATTCATTGTTGACAATAATGTCGGAGGATTCTATGAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0008150 GO:0008219 GO:0009626 GO:0009628 GO:0009651 GO:0009987 GO:0010337 GO:0010565 GO:0010817 GO:0012501 GO:0019222 GO:0031323 GO:0032350 GO:0033036 GO:0033037 GO:0033554 GO:0034050 GO:0045087 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051716 GO:0052542 GO:0052545 GO:0062012 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MACPF

Protein Analysis

603

Amino Acids

66.56

Weight (kDa)

8.9

Isoelectric Point (pI)

49.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MACPF	PF01823	104 - 316	8.2e-35	MAC/Perforin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010968)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28380
fragaria_vesca	FvH4_3g08740
malus_domestica	MD05G1311300.v1.1 MD10G1290300.v1.1
prunus_persica	Prupe.4G051500_v2.0.a1
pyrus_communis	pycom05g28740 pycom10g24250 pycom10g24280
rosa_chinensis	RchiOBHm_Chr5g0009001
rosa_laevigata	RLG00000031624
rosa_multiflora	Rmu_sc0021361.1_g000001
rosa_roxburghii	Rroxscaffold_1G00067010 Rroxscaffold_1G00067650
rosa_rugosa	Rorug04G0440800 Rorug04G0440900
rosa_samantha	Rh5AG069800 Rh5BG066500 Rh5CG077800 Rh5DG065800
rosa_wichuraiana	Rw5G006510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	155, 1786
AatII	GACGTC	2 cut(s)	186, 240
AccB1I	GGYRCC	1 cut(s)	1639
AccBSI	CCGCTC	1 cut(s)	231
AccII	CGCG	3 cut(s)	56, 104, 147
AciI	CCGC	6 cut(s)	56, 102, 145, 229, 731, 1474
AclWI	GGATC	1 cut(s)	664
AcsI	RAATTY	3 cut(s)	297, 577, 1597
AcyI	GRCGYC	2 cut(s)	183, 237
AdeI	CACNNNGTG	1 cut(s)	105
AfaI	GTAC	5 cut(s)	74, 514, 1244, 1346, 1555
AfiI	CCNNNNNNNGG	5 cut(s)	198, 741, 1025, 1148, 1753
AflIII	ACRYGT	1 cut(s)	1321
AgeI	ACCGGT	1 cut(s)	1141
AgsI	TTSAA	9 cut(s)	582, 840, 1120, 1169, 1295, 1400, 1452, 1564, 1597
AjiI	CACGTC	1 cut(s)	1007
AjnI	CCWGG	4 cut(s)	160, 221, 1391, 1699
AleI	CACNNNNGTG	1 cut(s)	599
AluBI	AGCT	6 cut(s)	38, 127, 359, 371, 1102, 1729
AluI	AGCT	6 cut(s)	38, 127, 359, 371, 1102, 1729
Alw26I	GTCTC	3 cut(s)	21, 190, 1049
AlwI	GGATC	1 cut(s)	664
Ama87I	CYCGRG	1 cut(s)	708
AoxI	GGCC	9 cut(s)	48, 732, 743, 1093, 1353, 1389, 1407, 1619, 1744
ApaI	GGGCCC	1 cut(s)	1097
ApeKI	GCWGC	4 cut(s)	38, 339, 356, 482
ApoI	RAATTY	3 cut(s)	297, 577, 1597
AsiGI	ACCGGT	1 cut(s)	1141
AspLEI	GCGC	2 cut(s)	219, 1053
AsuC2I	CCSGG	4 cut(s)	70, 139, 709, 710
AsuII	TTCGAA	1 cut(s)	793
AvaI	CYCGRG	1 cut(s)	708
AvaII	GGWCC	6 cut(s)	66, 141, 167, 475, 1493, 1685
AxyI	CCTNAGG	1 cut(s)	1689
BaeGI	GKGCMC	3 cut(s)	180, 885, 1097
BanI	GGYRCC	1 cut(s)	1639
BanII	GRGCYC	3 cut(s)	1018, 1097, 1263
BbvI	GCAGC	4 cut(s)	25, 326, 368, 469
BccI	CCATC	9 cut(s)	203, 377, 435, 662, 764, 1078, 1194, 1206, 1418
BceAI	ACGGC	2 cut(s)	35, 1572
BciT130I	CCWGG	4 cut(s)	162, 223, 1393, 1701
BciVI	GTATCC	1 cut(s)	1075
BclI	TGATCA	2 cut(s)	814, 1536
BcnI	CCSGG	4 cut(s)	70, 139, 709, 710
BcoDI	GTCTC	3 cut(s)	21, 190, 1049
BfmI	CTRYAG	1 cut(s)	1614
BfuI	GTATCC	1 cut(s)	1075
BglI	GCCNNNNNGGC	1 cut(s)	47
BisI	GCNGC	5 cut(s)	39, 340, 357, 483, 732
BlsI	GCNGC	5 cut(s)	40, 341, 358, 484, 733
Bme1390I	CCNGG	8 cut(s)	70, 139, 162, 223, 709, 710, 1393, 1701
Bme18I	GGWCC	6 cut(s)	66, 141, 167, 475, 1493, 1685
BmeT110I	CYCGRG	1 cut(s)	708
BmgBI	CACGTC	1 cut(s)	1007
BmrFI	CCNGG	8 cut(s)	70, 139, 162, 223, 709, 710, 1393, 1701
BmrI	ACTGGG	1 cut(s)	1331
BmsI	GCATC	4 cut(s)	343, 413, 1382, 1469
BmuI	ACTGGG	1 cut(s)	1331
BplI	GAGNNNNNCTC	2 cut(s)	116, 148
BpmI	CTGGAG	1 cut(s)	1187
Bpu14I	TTCGAA	1 cut(s)	793
BpuEI	CTTGAG	1 cut(s)	1456
BpuMI	CCSGG	4 cut(s)	70, 139, 709, 710
Bsa29I	ATCGAT	1 cut(s)	869
BsaBI	GATNNNNATC	1 cut(s)	813
BsaHI	GRCGYC	2 cut(s)	183, 237
BsaI	GGTCTC	2 cut(s)	21, 1049
BsaJI	CCNNGG	6 cut(s)	160, 707, 708, 910, 1404, 1668
BsaWI	WCCGGW	4 cut(s)	305, 1018, 1141, 1718
Bsc4I	CCNNNNNNNGG	5 cut(s)	198, 741, 1025, 1148, 1753
Bse118I	RCCGGY	1 cut(s)	1141
Bse1I	ACTGG	4 cut(s)	340, 658, 1135, 1337
Bse21I	CCTNAGG	1 cut(s)	1689
Bse8I	GATNNNNATC	1 cut(s)	813
BseBI	CCWGG	4 cut(s)	162, 223, 1393, 1701
BseCI	ATCGAT	1 cut(s)	869
BseDI	CCNNGG	6 cut(s)	160, 707, 708, 910, 1404, 1668
BseGI	GGATG	3 cut(s)	358, 589, 697
BseJI	GATNNNNATC	1 cut(s)	813
BseLI	CCNNNNNNNGG	5 cut(s)	198, 741, 1025, 1148, 1753
BseMII	CTCAG	3 cut(s)	483, 1060, 1680
BseNI	ACTGG	4 cut(s)	340, 658, 1135, 1337
BseSI	GKGCMC	3 cut(s)	180, 885, 1097
BseXI	GCAGC	4 cut(s)	25, 326, 368, 469
BseYI	CCCAGC	1 cut(s)	441
BsgI	GTGCAG	1 cut(s)	623
Bsh1236I	CGCG	3 cut(s)	56, 104, 147
Bsh1285I	CGRYCG	1 cut(s)	1142
BshFI	GGCC	9 cut(s)	50, 734, 745, 1095, 1355, 1391, 1409, 1621, 1746
BshNI	GGYRCC	1 cut(s)	1639
BshTI	ACCGGT	1 cut(s)	1141
BshVI	ATCGAT	1 cut(s)	869
BsiEI	CGRYCG	1 cut(s)	1142
BsiHKCI	CYCGRG	1 cut(s)	708
BsiSI	CCGG	8 cut(s)	69, 94, 139, 306, 709, 1019, 1142, 1719
BsiWI	CGTACG	1 cut(s)	1242
BslFI	GGGAC	4 cut(s)	143, 153, 268, 488
BslI	CCNNNNNNNGG	5 cut(s)	198, 741, 1025, 1148, 1753
BsmAI	GTCTC	3 cut(s)	21, 190, 1049
BsmBI	CGTCTC	1 cut(s)	190
BsmFI	GGGAC	4 cut(s)	143, 153, 268, 488
BsmI	GAATGC	1 cut(s)	1158
BsnI	GGCC	9 cut(s)	50, 734, 745, 1095, 1355, 1391, 1409, 1621, 1746
Bso31I	GGTCTC	2 cut(s)	21, 1049
BsoBI	CYCGRG	1 cut(s)	708
Bsp119I	TTCGAA	1 cut(s)	793
Bsp120I	GGGCCC	1 cut(s)	1093
Bsp1286I	GDGCHC	5 cut(s)	180, 885, 1018, 1097, 1263
Bsp143I	GATC	7 cut(s)	60, 656, 814, 1030, 1246, 1478, 1536
BspACI	CCGC	6 cut(s)	56, 102, 145, 229, 731, 1474
BspANI	GGCC	9 cut(s)	50, 734, 745, 1095, 1355, 1391, 1409, 1621, 1746
BspCNI	CTCAG	3 cut(s)	484, 1059, 1681
BspDI	ATCGAT	1 cut(s)	869
BspFNI	CGCG	3 cut(s)	56, 104, 147
BspMAI	CTGCAG	1 cut(s)	1618
BspPI	GGATC	1 cut(s)	664
BspT104I	TTCGAA	1 cut(s)	793
BspT107I	GGYRCC	1 cut(s)	1639
BspTNI	GGTCTC	2 cut(s)	21, 1049
BsrBI	CCGCTC	1 cut(s)	231
BsrFI	RCCGGY	1 cut(s)	1141
BsrI	ACTGG	4 cut(s)	340, 658, 1135, 1337
BssAI	RCCGGY	1 cut(s)	1141
BssECI	CCNNGG	6 cut(s)	160, 707, 708, 910, 1404, 1668
BssMI	GATC	7 cut(s)	60, 656, 814, 1030, 1246, 1478, 1536
BssNI	GRCGYC	2 cut(s)	183, 237
BssT1I	CCWWGG	3 cut(s)	910, 1404, 1668
Bst2UI	CCWGG	4 cut(s)	162, 223, 1393, 1701
Bst4CI	ACNGT	7 cut(s)	512, 754, 784, 844, 852, 1253, 1456
Bst6I	CTCTTC	1 cut(s)	1224
BstACI	GRCGYC	2 cut(s)	183, 237
BstBI	TTCGAA	1 cut(s)	793
BstC8I	GCNNGC	4 cut(s)	106, 215, 344, 729
BstDEI	CTNAG	5 cut(s)	492, 926, 950, 1046, 1689
BstEII	GGTNACC	1 cut(s)	1714
BstENI	CCTNNNNNAGG	1 cut(s)	196
BstF5I	GGATG	3 cut(s)	358, 589, 697
BstFNI	CGCG	3 cut(s)	56, 104, 147
BstHHI	GCGC	2 cut(s)	219, 1053
BstKTI	GATC	7 cut(s)	63, 659, 817, 1033, 1249, 1481, 1539
BstMAI	GTCTC	3 cut(s)	21, 190, 1049
BstMBI	GATC	7 cut(s)	60, 656, 814, 1030, 1246, 1478, 1536
BstMCI	CGRYCG	1 cut(s)	1142
BstMWI	GCNNNNNNNGC	4 cut(s)	47, 124, 210, 488
BstNI	CCWGG	4 cut(s)	162, 223, 1393, 1701
BstNSI	RCATGY	3 cut(s)	449, 731, 1325
BstPI	GGTNACC	1 cut(s)	1714
BstSCI	CCNGG	8 cut(s)	68, 137, 160, 221, 707, 708, 1391, 1699
BstSFI	CTRYAG	1 cut(s)	1614
BstSLI	GKGCMC	3 cut(s)	180, 885, 1097
BstUI	CGCG	3 cut(s)	56, 104, 147
BstV1I	GCAGC	4 cut(s)	25, 326, 368, 469
BstXI	CCANNNNNNTGG	3 cut(s)	917, 1011, 1707
Bsu15I	ATCGAT	1 cut(s)	869
Bsu36I	CCTNAGG	1 cut(s)	1689
BsuI	GTATCC	1 cut(s)	1075
BsuRI	GGCC	9 cut(s)	50, 734, 745, 1095, 1355, 1391, 1409, 1621, 1746
BsuTUI	ATCGAT	1 cut(s)	869
BtrI	CACGTC	1 cut(s)	1007
BtsCI	GGATG	3 cut(s)	358, 589, 697
BtsI	GCAGTG	3 cut(s)	942, 1073, 1329
BtsIMutI	CAGTG	4 cut(s)	333, 942, 1073, 1329
Cac8I	GCNNGC	4 cut(s)	106, 215, 344, 729
CfoI	GCGC	2 cut(s)	219, 1053
Cfr10I	RCCGGY	1 cut(s)	1141
Cfr9I	CCCGGG	1 cut(s)	708
ClaI	ATCGAT	1 cut(s)	869
CsiI	ACCWGGT	1 cut(s)	221
Csp6I	GTAC	5 cut(s)	73, 513, 1243, 1345, 1554
CspAI	ACCGGT	1 cut(s)	1141
CviAII	CATG	7 cut(s)	115, 446, 471, 562, 728, 932, 1322
CviQI	GTAC	5 cut(s)	73, 513, 1243, 1345, 1554
DdeI	CTNAG	5 cut(s)	492, 926, 950, 1046, 1689
DpnI	GATC	7 cut(s)	62, 658, 816, 1032, 1248, 1480, 1538
DpnII	GATC	7 cut(s)	60, 656, 814, 1030, 1246, 1478, 1536
DraIII	CACNNNGTG	1 cut(s)	105
DrdI	GACNNNNNNGTC	2 cut(s)	155, 1786
DseDI	GACNNNNNNGTC	2 cut(s)	155, 1786
Eam1104I	CTCTTC	1 cut(s)	1224
EarI	CTCTTC	1 cut(s)	1224
Eco130I	CCWWGG	3 cut(s)	910, 1404, 1668
Eco147I	AGGCCT	1 cut(s)	1391
Eco24I	GRGCYC	3 cut(s)	1018, 1097, 1263
Eco31I	GGTCTC	2 cut(s)	21, 1049
Eco32I	GATATC	1 cut(s)	91
Eco47I	GGWCC	6 cut(s)	66, 141, 167, 475, 1493, 1685
Eco81I	CCTNAGG	1 cut(s)	1689
Eco88I	CYCGRG	1 cut(s)	708
Eco91I	GGTNACC	1 cut(s)	1714
EcoNI	CCTNNNNNAGG	1 cut(s)	196
EcoO109I	RGGNCCY	4 cut(s)	475, 1353, 1493, 1685
EcoO65I	GGTNACC	1 cut(s)	1714
EcoRI	GAATTC	1 cut(s)	1597
EcoRII	CCWGG	4 cut(s)	160, 221, 1391, 1699
EcoRV	GATATC	1 cut(s)	91
EcoT14I	CCWWGG	3 cut(s)	910, 1404, 1668
EcoT38I	GRGCYC	3 cut(s)	1018, 1097, 1263
ErhI	CCWWGG	3 cut(s)	910, 1404, 1668
Esp3I	CGTCTC	1 cut(s)	190
FaeI	CATG	7 cut(s)	118, 449, 474, 565, 731, 935, 1325
FaqI	GGGAC	4 cut(s)	143, 153, 268, 488
FatI	CATG	7 cut(s)	114, 445, 470, 561, 727, 931, 1321
FauI	CCCGC	1 cut(s)	152
FbaI	TGATCA	2 cut(s)	814, 1536
Fnu4HI	GCNGC	5 cut(s)	39, 340, 357, 483, 732
FokI	GGATG	3 cut(s)	365, 576, 704
FriOI	GRGCYC	3 cut(s)	1018, 1097, 1263
Fsp4HI	GCNGC	5 cut(s)	39, 340, 357, 483, 732
GlaI	GCGC	2 cut(s)	218, 1052
GluI	GCNGC	5 cut(s)	39, 340, 357, 483, 732
GsaI	CCCAGC	1 cut(s)	445
GsuI	CTGGAG	1 cut(s)	1187
HaeIII	GGCC	9 cut(s)	50, 734, 745, 1095, 1355, 1391, 1409, 1621, 1746
HapII	CCGG	8 cut(s)	69, 94, 139, 306, 709, 1019, 1142, 1719
HhaI	GCGC	2 cut(s)	219, 1053
Hin1I	GRCGYC	2 cut(s)	183, 237
Hin1II	CATG	7 cut(s)	118, 449, 474, 565, 731, 935, 1325
Hin6I	GCGC	2 cut(s)	217, 1051
HinP1I	GCGC	2 cut(s)	217, 1051
HincII	GTYRAC	2 cut(s)	1111, 1779
HindII	GTYRAC	2 cut(s)	1111, 1779
HindIII	AAGCTT	1 cut(s)	1727
HpaI	GTTAAC	1 cut(s)	1111
HpaII	CCGG	8 cut(s)	69, 94, 139, 306, 709, 1019, 1142, 1719
Hpy166II	GTNNAC	4 cut(s)	559, 1111, 1609, 1779
Hpy188III	TCNNGA	7 cut(s)	678, 1166, 1223, 1435, 1509, 1518, 1688
Hpy8I	GTNNAC	4 cut(s)	559, 1111, 1609, 1779
Hpy99I	CGWCG	8 cut(s)	56, 158, 176, 185, 239, 242, 1504, 1657
HpyCH4III	ACNGT	7 cut(s)	512, 754, 784, 844, 852, 1253, 1456
HpyCH4IV	ACGT	4 cut(s)	183, 237, 1006, 1655
HpyF10VI	GCNNNNNNNGC	4 cut(s)	47, 124, 210, 488
HpyF3I	CTNAG	5 cut(s)	492, 926, 950, 1046, 1689
HpySE526I	ACGT	4 cut(s)	183, 237, 1006, 1655
Hsp92I	GRCGYC	2 cut(s)	183, 237
Hsp92II	CATG	7 cut(s)	118, 449, 474, 565, 731, 935, 1325
HspAI	GCGC	2 cut(s)	217, 1051
KflI	GGGWCCC	1 cut(s)	475
Ksp22I	TGATCA	2 cut(s)	814, 1536
KspAI	GTTAAC	1 cut(s)	1111
Kzo9I	GATC	7 cut(s)	60, 656, 814, 1030, 1246, 1478, 1536
LmnI	GCTCC	6 cut(s)	26, 236, 364, 1021, 1308, 1721
Lsp1109I	GCAGC	4 cut(s)	25, 326, 368, 469
LweI	GCATC	4 cut(s)	343, 413, 1382, 1469
MabI	ACCWGGT	1 cut(s)	221
MaeII	ACGT	4 cut(s)	183, 237, 1006, 1655
MaeIII	GTNAC	9 cut(s)	570, 852, 999, 1144, 1181, 1253, 1381, 1573, 1714
MalI	GATC	7 cut(s)	62, 658, 816, 1032, 1248, 1480, 1538
MbiI	CCGCTC	1 cut(s)	231
MboI	GATC	7 cut(s)	60, 656, 814, 1030, 1246, 1478, 1536
MboII	GAAGA	3 cut(s)	578, 1025, 1211
MfeI	CAATTG	1 cut(s)	1377
MhlI	GDGCHC	5 cut(s)	180, 885, 1018, 1097, 1263
MlyI	GAGTC	5 cut(s)	125, 671, 692, 1361, 1688
MmeI	TCCRAC	6 cut(s)	48, 258, 386, 1003, 1258, 1770
MseI	TTAA	6 cut(s)	249, 315, 327, 614, 1110, 1807
MslI	CAYNNNNRTG	6 cut(s)	599, 869, 1009, 1088, 1324, 1415
MspA1I	CMGCKG	1 cut(s)	38
MspI	CCGG	8 cut(s)	69, 94, 139, 306, 709, 1019, 1142, 1719
MspR9I	CCNGG	8 cut(s)	70, 139, 162, 223, 709, 710, 1393, 1701
MunI	CAATTG	1 cut(s)	1377
Mva1269I	GAATGC	1 cut(s)	1158
MvaI	CCWGG	4 cut(s)	162, 223, 1393, 1701
MvnI	CGCG	3 cut(s)	56, 104, 147
MwoI	GCNNNNNNNGC	4 cut(s)	47, 124, 210, 488
NciI	CCSGG	4 cut(s)	70, 139, 709, 710
NdeII	GATC	7 cut(s)	60, 656, 814, 1030, 1246, 1478, 1536
NlaIII	CATG	7 cut(s)	118, 449, 474, 565, 731, 935, 1325
NmeAIII	GCCGAG	1 cut(s)	67
NmuCI	GTSAC	6 cut(s)	852, 1144, 1181, 1381, 1573, 1714
NspI	RCATGY	3 cut(s)	449, 731, 1325
NspV	TTCGAA	1 cut(s)	793
OliI	CACNNNNGTG	1 cut(s)	599
PaeI	GCATGC	1 cut(s)	731
PceI	AGGCCT	1 cut(s)	1391
PciI	ACATGT	1 cut(s)	1321
PcsI	WCGNNNNNNNCGW	1 cut(s)	180
PctI	GAATGC	1 cut(s)	1158
PfeI	GAWTC	5 cut(s)	940, 1521, 1564, 1749, 1796
Pfl23II	CGTACG	1 cut(s)	1242
PflFI	GACNNNGTC	1 cut(s)	152
PinAI	ACCGGT	1 cut(s)	1141
PkrI	GCNGC	5 cut(s)	40, 341, 358, 484, 733
PleI	GAGTC	5 cut(s)	125, 670, 692, 1361, 1688
PpsI	GAGTC	5 cut(s)	125, 670, 692, 1361, 1688
PpuMI	RGGWCCY	3 cut(s)	475, 1493, 1685
PscI	ACATGT	1 cut(s)	1321
Psp5II	RGGWCCY	3 cut(s)	475, 1493, 1685
Psp6I	CCWGG	4 cut(s)	160, 221, 1391, 1699
PspEI	GGTNACC	1 cut(s)	1714
PspFI	CCCAGC	1 cut(s)	441
PspGI	CCWGG	4 cut(s)	160, 221, 1391, 1699
PspLI	CGTACG	1 cut(s)	1242
PspOMI	GGGCCC	1 cut(s)	1093
PspPPI	RGGWCCY	3 cut(s)	475, 1493, 1685
PstI	CTGCAG	1 cut(s)	1618
PsyI	GACNNNGTC	1 cut(s)	152
PvuII	CAGCTG	1 cut(s)	38
RsaI	GTAC	5 cut(s)	74, 514, 1244, 1346, 1555
RsaNI	GTAC	5 cut(s)	73, 513, 1243, 1345, 1554
RseI	CAYNNNNRTG	6 cut(s)	599, 869, 1009, 1088, 1324, 1415
SaqAI	TTAA	6 cut(s)	249, 315, 327, 614, 1110, 1807
SatI	GCNGC	5 cut(s)	39, 340, 357, 483, 732
Sau3AI	GATC	7 cut(s)	60, 656, 814, 1030, 1246, 1478, 1536
SbfI	CCTGCAGG	1 cut(s)	1618
SchI	GAGTC	5 cut(s)	125, 671, 692, 1361, 1688
ScrFI	CCNGG	8 cut(s)	70, 139, 162, 223, 709, 710, 1393, 1701
SdaI	CCTGCAGG	1 cut(s)	1618
SduI	GDGCHC	5 cut(s)	180, 885, 1018, 1097, 1263
SexAI	ACCWGGT	1 cut(s)	221
SfaNI	GCATC	4 cut(s)	343, 413, 1382, 1469
SfcI	CTRYAG	1 cut(s)	1614
SfuI	TTCGAA	1 cut(s)	793
SinI	GGWCC	6 cut(s)	66, 141, 167, 475, 1493, 1685
SmaI	CCCGGG	1 cut(s)	710
SmiMI	CAYNNNNRTG	6 cut(s)	599, 869, 1009, 1088, 1324, 1415
SmlI	CTYRAG	1 cut(s)	1435
SmoI	CTYRAG	1 cut(s)	1435
SphI	GCATGC	1 cut(s)	731
Sse8387I	CCTGCAGG	1 cut(s)	1618
SseBI	AGGCCT	1 cut(s)	1391
SsiI	CCGC	6 cut(s)	56, 102, 145, 229, 731, 1474
StuI	AGGCCT	1 cut(s)	1391
StyD4I	CCNGG	8 cut(s)	68, 137, 160, 221, 707, 708, 1391, 1699
StyI	CCWWGG	3 cut(s)	910, 1404, 1668
TaaI	ACNGT	7 cut(s)	512, 754, 784, 844, 852, 1253, 1456
TaiI	ACGT	4 cut(s)	186, 240, 1009, 1658
TaqI	TCGA	7 cut(s)	123, 134, 793, 869, 1138, 1499, 1510
TaqII	GACCGA	1 cut(s)	1031
TatI	WGTACW	1 cut(s)	1344
TauI	GCSGC	1 cut(s)	734
TfiI	GAWTC	5 cut(s)	940, 1521, 1564, 1749, 1796
Tru1I	TTAA	6 cut(s)	249, 315, 327, 614, 1110, 1807
Tru9I	TTAA	6 cut(s)	249, 315, 327, 614, 1110, 1807
TscAI	CASTG	4 cut(s)	340, 942, 1080, 1336
TseFI	GTSAC	6 cut(s)	852, 1144, 1181, 1381, 1573, 1714
TseI	GCWGC	4 cut(s)	38, 339, 356, 482
Tsp45I	GTSAC	6 cut(s)	852, 1144, 1181, 1381, 1573, 1714
TspDTI	ATGAA	5 cut(s)	487, 578, 578, 1513, 1761
TspGWI	ACGGA	1 cut(s)	291
TspMI	CCCGGG	1 cut(s)	708
TspRI	CASTG	4 cut(s)	340, 942, 1080, 1336
Tth111I	GACNNNGTC	1 cut(s)	152
VpaK11BI	GGWCC	6 cut(s)	66, 141, 167, 475, 1493, 1685
XagI	CCTNNNNNAGG	1 cut(s)	196
XapI	RAATTY	3 cut(s)	297, 577, 1597
XceI	RCATGY	3 cut(s)	449, 731, 1325
XmaI	CCCGGG	1 cut(s)	708
ZraI	GACGTC	2 cut(s)	184, 238

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.