FvH4_3g09380

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0007493
Physical Location & Seq
Reverse (-)
5471366 .. 5474990
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09380.t1

Sequence Viewer

Length: 1380 bp
ATGGAGGCTTCTGGGTTCAACGGCGCTGCGTTTTCCATCGACGAAATCGCTTCTGCTTATTGCAAGGCCGGGAAGAACGCCGCGGCGGCCGCCGAGACTCTCGCTCTCTCCGGCCGTGAAGAAGCTTCTGGCGATATTGGGAAGAAGAACAAAACGACGTCGGATAATGTGAGTGGGAATTCGAGGGGTTCCAAGACGAAGTATCGGCCGGTTTCAATGGGGAGTGTGTCTGATGTGATTGGGAAACAATATGGGAGTAAAACGACGTCGGGTTGTAATGGAGGGAAGGCATTGAGAGTGGACTCAAAGGCCAAGGTGCTGCCGATTCCGGAGAGCTGGGCGGAAAAGGCGGAGTCTTGTTCATCTGGGGATGATGTGCTTCAACAGGACATGGAGGATTTTCTCTTCACTATGCTTGGAGAAGGGTTCAAGCTCGATAGGGATTTGATTCGAGATGTTCTTGATAGTTGCGGATATGATATGGACAAGAGCATGGAAAATCTTATTAGTTTGTCAACATCAGCCTCGGATGAAAGAAACGAAGTGATCTGTAACTCCAATGATAAGATCCAGTCTGCAGGCTCATGTCTAAAAGTGGAAGTATTTACTCAGGGAGAGCACATAAACTCTACTGAAATTAATGGAGATAGGGCTTCAAATACAAATGGAGTAGAATCAATTGGAAAAGAAATTTTGGCTGCTCTCTTCAGTGCTCCTGAGATACCTGAGGAGCCTGAGCTACCAAAAAAAAGAACAGTAAGACCAACAAGTAGATATGCAGCCTACGGCCATTTGGTGGTGGAACCTCCAACAGATACGTTTTTAGATAGCAGGACACCTGTGGTCTATCAACAGCATCACATTGAGGATGATGCAGATGAAGAAGATAGGTACCAAGCTCTTCGAAGAAGTGTGAAGGAGTATCGGAGTACAATGAAGGAGTATTACCAGGCAGCTGTAGAAGCATTTTCCAAGGGAGATCGTGTTCGAGCTGACAAACTTATGGAACAGGGACACTTTTTCCAGAAAAAGGCACGGGATGCAGATGAGGAGTCAAATAAAATGATCTTTAAACCTGGAAGTGCTGAGACACAAGGGGATATAGTGCTTGACTTGCATGAAGGTGGTGCCAAGGAGGCGATACGTCTTCTGAAGTGTCATCTTTCTTCAGTTGCTGGTATCTCATCAATCAAGCATCTTAAGGTCATTATTGATACAAAGGAGAGAGATATCAAAAAAGGATCTCGGAGACGATTAGCAGTTGTGAGGCTTTTGGAGGAAGAATCGATCAAGTGGGTTGAGGCAGAAAATGCTGGAACACTACTGATTCAGTTGGACAGTATCAACCCAAAGAAGTTAAAGTTTCTAAAAAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771

Protein Analysis

460

Amino Acids

50.47

Weight (kDa)

5.63

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 298 - 358 2.8e-14 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 161, 269
Acc65I GGTACC 1 cut(s) 891
AccB1I GGYRCC 2 cut(s) 891, 1127
AccB7I CCANNNNNTGG 1 cut(s) 796
AccII CGCG 1 cut(s) 83
AccIII TCCGGA 1 cut(s) 328
AciI CCGC 7 cut(s) 81, 83, 86, 90, 341, 350, 471
AclWI GGATC 2 cut(s) 562, 1249
AcoI YGGCCR 4 cut(s) 87, 112, 206, 787
AcsI RAATTY 2 cut(s) 178, 690
AcuI CTGAAG 3 cut(s) 691, 1152, 1172
AcyI GRCGYC 2 cut(s) 158, 266
AfaI GTAC 2 cut(s) 893, 931
AfiI CCNNNNNNNGG 2 cut(s) 796, 1030
AflII CTTAAG 1 cut(s) 1199
AgsI TTSAA 5 cut(s) 19, 216, 383, 430, 657
AjnI CCWGG 2 cut(s) 948, 1075
AloI GAACNNNNNNTCC 2 cut(s) 969, 1001
AluBI AGCT 7 cut(s) 125, 336, 433, 739, 899, 956, 992
AluI AGCT 7 cut(s) 125, 336, 433, 739, 899, 956, 992
Alw21I GWGCWC 2 cut(s) 621, 715
Alw26I GTCTC 3 cut(s) 89, 1082, 1243
AlwI GGATC 2 cut(s) 562, 1249
AlwNI CAGNNNCTG 1 cut(s) 1175
Aor13HI TCCGGA 1 cut(s) 328
AoxI GGCC 6 cut(s) 66, 87, 112, 206, 309, 787
ApeKI GCWGC 5 cut(s) 26, 319, 698, 779, 953
ApoI RAATTY 2 cut(s) 178, 690
AseI ATTAAT 1 cut(s) 639
Asp718I GGTACC 1 cut(s) 891
AspLEI GCGC 1 cut(s) 26
AsuC2I CCSGG 1 cut(s) 70
AsuII TTCGAA 1 cut(s) 904
AxyI CCTNAGG 1 cut(s) 726
BanI GGYRCC 2 cut(s) 891, 1127
BarI GAAGNNNNNNTAC 2 cut(s) 929, 961
BbsI GAAGAC 1 cut(s) 1139
Bbv12I GWGCWC 2 cut(s) 621, 715
BbvI GCAGC 5 cut(s) 13, 306, 685, 791, 965
BccI CCATC 1 cut(s) 44
BceAI ACGGC 3 cut(s) 37, 99, 802
BciT130I CCWGG 2 cut(s) 950, 1077
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 3 cut(s) 89, 1082, 1243
BfmI CTRYAG 2 cut(s) 576, 957
BfoI RGCGCY 1 cut(s) 27
BfrI CTTAAG 1 cut(s) 1199
BglI GCCNNNNNGGC 2 cut(s) 86, 1136
BisI GCNGC 9 cut(s) 27, 81, 84, 87, 90, 320, 699, 780, 954
BlsI GCNGC 9 cut(s) 28, 82, 85, 88, 91, 321, 700, 781, 955
Bme1390I CCNGG 3 cut(s) 70, 950, 1077
BmiI GGNNCC 5 cut(s) 190, 732, 804, 893, 1129
BmrFI CCNGG 3 cut(s) 70, 950, 1077
BmsI GCATC 4 cut(s) 862, 865, 1030, 1204
BpiI GAAGAC 1 cut(s) 1139
Bpu10I CCTNAGC 1 cut(s) 735
Bpu14I TTCGAA 1 cut(s) 904
BpuMI CCSGG 1 cut(s) 70
Bsa29I ATCGAT 1 cut(s) 1286
BsaHI GRCGYC 2 cut(s) 158, 266
BsaJI CCNNGG 5 cut(s) 81, 312, 525, 972, 1131
BsaWI WCCGGW 1 cut(s) 328
BsaXI ACNNNNNCTCC 2 cut(s) 969, 999
Bsc4I CCNNNNNNNGG 2 cut(s) 796, 1030
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 571
Bse21I CCTNAGG 1 cut(s) 726
BseAI TCCGGA 1 cut(s) 328
BseBI CCWGG 2 cut(s) 950, 1077
BseCI ATCGAT 1 cut(s) 1286
BseDI CCNNGG 5 cut(s) 81, 312, 525, 972, 1131
BseGI GGATG 4 cut(s) 376, 535, 874, 1045
BseLI CCNNNNNNNGG 2 cut(s) 796, 1030
BseMII CTCAG 5 cut(s) 623, 708, 717, 726, 1077
BseNI ACTGG 1 cut(s) 571
BseRI GAGGAG 2 cut(s) 743, 1064
BseX3I CGGCCG 3 cut(s) 87, 112, 206
BseXI GCAGC 5 cut(s) 13, 306, 685, 791, 965
BseYI CCCAGC 1 cut(s) 336
Bsh1236I CGCG 1 cut(s) 83
Bsh1285I CGRYCG 3 cut(s) 90, 115, 209
BshFI GGCC 6 cut(s) 68, 89, 114, 208, 311, 789
BshNI GGYRCC 2 cut(s) 891, 1127
BshVI ATCGAT 1 cut(s) 1286
BsiEI CGRYCG 3 cut(s) 90, 115, 209
BsiHKAI GWGCWC 2 cut(s) 621, 715
BsiSI CCGG 4 cut(s) 69, 111, 209, 329
BslFI GGGAC 1 cut(s) 1026
BslI CCNNNNNNNGG 2 cut(s) 796, 1030
BsmAI GTCTC 3 cut(s) 89, 1082, 1243
BsmBI CGTCTC 1 cut(s) 1243
BsmFI GGGAC 1 cut(s) 1026
BsnI GGCC 6 cut(s) 68, 89, 114, 208, 311, 789
Bsp119I TTCGAA 1 cut(s) 904
Bsp1286I GDGCHC 2 cut(s) 621, 715
Bsp13I TCCGGA 1 cut(s) 328
Bsp143I GATC 6 cut(s) 546, 567, 979, 1065, 1241, 1287
BspACI CCGC 7 cut(s) 81, 83, 86, 90, 341, 350, 471
BspANI GGCC 6 cut(s) 68, 89, 114, 208, 311, 789
BspCNI CTCAG 5 cut(s) 622, 709, 718, 727, 1078
BspDI ATCGAT 1 cut(s) 1286
BspEI TCCGGA 1 cut(s) 328
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 5 cut(s) 190, 732, 804, 893, 1129
BspMAI CTGCAG 1 cut(s) 580
BspPI GGATC 2 cut(s) 562, 1249
BspQI GCTCTTC 1 cut(s) 906
BspT104I TTCGAA 1 cut(s) 904
BspT107I GGYRCC 2 cut(s) 891, 1127
BspTI CTTAAG 1 cut(s) 1199
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 1 cut(s) 571
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 5 cut(s) 81, 312, 525, 972, 1131
BssMI GATC 6 cut(s) 546, 567, 979, 1065, 1241, 1287
BssNI GRCGYC 2 cut(s) 158, 266
BssT1I CCWWGG 3 cut(s) 312, 972, 1131
Bst2UI CCWGG 2 cut(s) 950, 1077
Bst4CI ACNGT 2 cut(s) 757, 1340
Bst6I CTCTTC 3 cut(s) 410, 710, 906
BstACI GRCGYC 2 cut(s) 158, 266
BstAFI CTTAAG 1 cut(s) 1199
BstAPI GCANNNNNTGC 2 cut(s) 1040, 1310
BstBI TTCGAA 1 cut(s) 904
BstC8I GCNNGC 1 cut(s) 580
BstDEI CTNAG 5 cut(s) 609, 717, 726, 735, 1086
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 4 cut(s) 376, 535, 874, 1045
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 6 cut(s) 549, 570, 982, 1068, 1244, 1290
BstMAI GTCTC 3 cut(s) 89, 1082, 1243
BstMBI GATC 6 cut(s) 546, 567, 979, 1065, 1241, 1287
BstMCI CGRYCG 3 cut(s) 90, 115, 209
BstMWI GCNNNNNNNGC 8 cut(s) 86, 89, 347, 962, 1040, 1114, 1136, 1310
BstNI CCWGG 2 cut(s) 950, 1077
BstSCI CCNGG 3 cut(s) 68, 948, 1075
BstSFI CTRYAG 2 cut(s) 576, 957
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 5 cut(s) 13, 306, 685, 791, 965
BstV2I GAAGAC 1 cut(s) 1139
BstX2I RGATCY 2 cut(s) 567, 1241
BstYI RGATCY 2 cut(s) 567, 1241
BstZI CGGCCG 3 cut(s) 87, 112, 206
Bsu15I ATCGAT 1 cut(s) 1286
Bsu36I CCTNAGG 1 cut(s) 726
BsuRI GGCC 6 cut(s) 68, 89, 114, 208, 311, 789
BsuTUI ATCGAT 1 cut(s) 1286
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 4 cut(s) 376, 535, 874, 1045
BtsIMutI CAGTG 1 cut(s) 715
Cac8I GCNNGC 1 cut(s) 580
CaiI CAGNNNCTG 1 cut(s) 1175
CciNI GCGGCCGC 1 cut(s) 87
CfoI GCGC 1 cut(s) 26
Cfr10I RCCGGY 1 cut(s) 208
Cfr42I CCGCGG 1 cut(s) 84
ClaI ATCGAT 1 cut(s) 1286
Csp6I GTAC 2 cut(s) 892, 930
CviAII CATG 4 cut(s) 391, 493, 585, 1118
CviQI GTAC 2 cut(s) 892, 930
DdeI CTNAG 5 cut(s) 609, 717, 726, 735, 1086
DpnI GATC 6 cut(s) 548, 569, 981, 1067, 1243, 1289
DpnII GATC 6 cut(s) 546, 567, 979, 1065, 1241, 1287
DraI TTTAAA 1 cut(s) 1072
EaeI YGGCCR 4 cut(s) 87, 112, 206, 787
EagI CGGCCG 3 cut(s) 87, 112, 206
Eam1104I CTCTTC 3 cut(s) 410, 710, 906
EarI CTCTTC 3 cut(s) 410, 710, 906
EciI GGCGGA 2 cut(s) 356, 365
EclXI CGGCCG 3 cut(s) 87, 112, 206
Eco130I CCWWGG 3 cut(s) 312, 972, 1131
Eco32I GATATC 1 cut(s) 1231
Eco52I CGGCCG 3 cut(s) 87, 112, 206
Eco57I CTGAAG 3 cut(s) 691, 1152, 1172
Eco81I CCTNAGG 1 cut(s) 726
EcoRI GAATTC 1 cut(s) 178
EcoRII CCWGG 2 cut(s) 948, 1075
EcoRV GATATC 1 cut(s) 1231
EcoT14I CCWWGG 3 cut(s) 312, 972, 1131
ErhI CCWWGG 3 cut(s) 312, 972, 1131
Esp3I CGTCTC 1 cut(s) 1243
FaeI CATG 4 cut(s) 394, 496, 588, 1121
FaqI GGGAC 1 cut(s) 1026
FatI CATG 4 cut(s) 390, 492, 584, 1117
Fnu4HI GCNGC 9 cut(s) 27, 81, 84, 87, 90, 320, 699, 780, 954
FokI GGATG 4 cut(s) 383, 542, 881, 1052
Fsp4HI GCNGC 9 cut(s) 27, 81, 84, 87, 90, 320, 699, 780, 954
GlaI GCGC 1 cut(s) 25
GluI GCNGC 9 cut(s) 27, 81, 84, 87, 90, 320, 699, 780, 954
GsaI CCCAGC 1 cut(s) 340
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 6 cut(s) 68, 89, 114, 208, 311, 789
HapII CCGG 4 cut(s) 69, 111, 209, 329
HhaI GCGC 1 cut(s) 26
Hin1I GRCGYC 2 cut(s) 158, 266
Hin1II CATG 4 cut(s) 394, 496, 588, 1121
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HincII GTYRAC 1 cut(s) 516
HindII GTYRAC 1 cut(s) 516
HindIII AAGCTT 1 cut(s) 123
HinfI GANTC 9 cut(s) 97, 302, 325, 353, 448, 674, 1052, 1283, 1327
HpaII CCGG 4 cut(s) 69, 111, 209, 329
Hpy166II GTNNAC 2 cut(s) 301, 516
Hpy188I TCNGA 6 cut(s) 163, 232, 529, 927, 1152, 1248
Hpy188III TCNNGA 5 cut(s) 329, 452, 461, 716, 1024
Hpy8I GTNNAC 2 cut(s) 301, 516
Hpy99I CGWCG 5 cut(s) 44, 160, 163, 268, 271
HpyAV CCTTC 5 cut(s) 280, 416, 910, 931, 1115
HpyCH4III ACNGT 2 cut(s) 757, 1340
HpyCH4IV ACGT 4 cut(s) 158, 266, 818, 1144
HpyCH4V TGCA 6 cut(s) 63, 578, 779, 875, 1043, 1117
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 89, 347, 962, 1040, 1114, 1136, 1310
HpyF3I CTNAG 5 cut(s) 609, 717, 726, 735, 1086
HpySE526I ACGT 4 cut(s) 158, 266, 818, 1144
Hsp92I GRCGYC 2 cut(s) 158, 266
Hsp92II CATG 4 cut(s) 394, 496, 588, 1121
HspAI GCGC 1 cut(s) 24
Kpn2I TCCGGA 1 cut(s) 328
KpnI GGTACC 1 cut(s) 895
KspI CCGCGG 1 cut(s) 84
Kzo9I GATC 6 cut(s) 546, 567, 979, 1065, 1241, 1287
LguI GCTCTTC 1 cut(s) 906
LmnI GCTCC 2 cut(s) 718, 730
Lsp1109I GCAGC 5 cut(s) 13, 306, 685, 791, 965
LweI GCATC 4 cut(s) 862, 865, 1030, 1204
MaeII ACGT 4 cut(s) 158, 266, 818, 1144
MaeIII GTNAC 1 cut(s) 551
MalI GATC 6 cut(s) 548, 569, 981, 1067, 1243, 1289
MboI GATC 6 cut(s) 546, 567, 979, 1065, 1241, 1287
MfeI CAATTG 1 cut(s) 678
MflI RGATCY 2 cut(s) 567, 1241
MhlI GDGCHC 2 cut(s) 621, 715
MluCI AATT 4 cut(s) 178, 636, 678, 690
MlyI GAGTC 4 cut(s) 91, 296, 362, 1061
MmeI TCCRAC 3 cut(s) 141, 833, 1314
MroI TCCGGA 1 cut(s) 328
MseI TTAA 4 cut(s) 639, 1071, 1200, 1358
MslI CAYNNNNRTG 1 cut(s) 1122
MspA1I CMGCKG 2 cut(s) 83, 956
MspCI CTTAAG 1 cut(s) 1199
MspI CCGG 4 cut(s) 69, 111, 209, 329
MspR9I CCNGG 3 cut(s) 70, 950, 1077
MunI CAATTG 1 cut(s) 678
MvaI CCWGG 2 cut(s) 950, 1077
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 8 cut(s) 86, 89, 347, 962, 1040, 1114, 1136, 1310
NciI CCSGG 1 cut(s) 70
NdeII GATC 6 cut(s) 546, 567, 979, 1065, 1241, 1287
NlaIII CATG 4 cut(s) 394, 496, 588, 1121
NlaIV GGNNCC 5 cut(s) 190, 732, 804, 893, 1129
NmeAIII GCCGAG 1 cut(s) 118
NotI GCGGCCGC 1 cut(s) 87
NspV TTCGAA 1 cut(s) 904
PciSI GCTCTTC 1 cut(s) 906
PfeI GAWTC 5 cut(s) 325, 448, 674, 1283, 1327
PflMI CCANNNNNTGG 1 cut(s) 796
PkrI GCNGC 9 cut(s) 28, 82, 85, 88, 91, 321, 700, 781, 955
PleI GAGTC 4 cut(s) 91, 296, 361, 1060
PpsI GAGTC 4 cut(s) 91, 296, 361, 1060
PshBI ATTAAT 1 cut(s) 639
Psp6I CCWGG 2 cut(s) 948, 1075
PspFI CCCAGC 1 cut(s) 336
PspGI CCWGG 2 cut(s) 948, 1075
PspN4I GGNNCC 5 cut(s) 190, 732, 804, 893, 1129
PstI CTGCAG 1 cut(s) 580
PstNI CAGNNNCTG 1 cut(s) 1175
PsuI RGATCY 2 cut(s) 567, 1241
PvuII CAGCTG 1 cut(s) 956
RsaI GTAC 2 cut(s) 893, 931
RsaNI GTAC 2 cut(s) 892, 930
RseI CAYNNNNRTG 1 cut(s) 1122
SacII CCGCGG 1 cut(s) 84
SapI GCTCTTC 1 cut(s) 906
SaqAI TTAA 4 cut(s) 639, 1071, 1200, 1358
SatI GCNGC 9 cut(s) 27, 81, 84, 87, 90, 320, 699, 780, 954
Sau3AI GATC 6 cut(s) 546, 567, 979, 1065, 1241, 1287
SchI GAGTC 4 cut(s) 91, 296, 362, 1061
ScrFI CCNGG 3 cut(s) 70, 950, 1077
SduI GDGCHC 2 cut(s) 621, 715
SfaNI GCATC 4 cut(s) 862, 865, 1030, 1204
SfcI CTRYAG 2 cut(s) 576, 957
Sfr303I CCGCGG 1 cut(s) 84
SfuI TTCGAA 1 cut(s) 904
SgrBI CCGCGG 1 cut(s) 84
SmiMI CAYNNNNRTG 1 cut(s) 1122
SmlI CTYRAG 1 cut(s) 1199
SmoI CTYRAG 1 cut(s) 1199
Sse9I AATT 4 cut(s) 178, 636, 678, 690
SsiI CCGC 7 cut(s) 81, 83, 86, 90, 341, 350, 471
StyD4I CCNGG 3 cut(s) 68, 948, 1075
StyI CCWWGG 3 cut(s) 312, 972, 1131
TaaI ACNGT 2 cut(s) 757, 1340
TaiI ACGT 4 cut(s) 161, 269, 821, 1147
TaqI TCGA 7 cut(s) 39, 182, 435, 451, 904, 988, 1286
TasI AATT 4 cut(s) 178, 636, 678, 690
TatI WGTACW 1 cut(s) 929
TauI GCSGC 4 cut(s) 83, 86, 89, 92
TfiI GAWTC 5 cut(s) 325, 448, 674, 1283, 1327
Tru1I TTAA 4 cut(s) 639, 1071, 1200, 1358
Tru9I TTAA 4 cut(s) 639, 1071, 1200, 1358
TscAI CASTG 1 cut(s) 715
TseI GCWGC 5 cut(s) 26, 319, 698, 779, 953
TspDTI ATGAA 5 cut(s) 351, 546, 894, 950, 1134
TspRI CASTG 1 cut(s) 715
Van91I CCANNNNNTGG 1 cut(s) 796
Vha464I CTTAAG 1 cut(s) 1199
VspI ATTAAT 1 cut(s) 639
XapI RAATTY 2 cut(s) 178, 690
ZraI GACGTC 2 cut(s) 159, 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.