Rroxscaffold_1G00068270

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0007493
Physical Location & Seq
Reverse (-)
89503930 .. 89508970
5041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00068270.1

Sequence Viewer

Length: 789 bp
ATGGAAGCTTCTGGTTTCAATGGTGCCCCCAGATTCGACGACGAACAGGGCTTGAGGGTTTTGCTCGACGCCTTTCGCCCTATGTTTTCCACTGAGGAAATCGCTTCGGCTTATTGCAAGGCCGGGAAGAACGCCGAGGCCGCCGCCGAGACTCTGGCTCTCTCCGCCACCAGCGGCGGCGAAGAGTCGTCGGGAGTAGTAGCGGATGATGGTGAGATTGGGAAGAAGAGCAAAACGACGTCGGATAATGGGAGTGGGAATTCGAGGGGTGCGAAGACGAAGTATCGGTCGGTTTCGATGGGGAGTGTTTCTGGTGTTATTGGGAAGCATTATGGGAACAAAACGACGTCGTCGGGTGGTAATGGAGGGAAGGGCTTGAGAGTGGACTCAAAGTCCAAGGTGTTGCCGATTCCGGAGAGCTGGGTGGAAAAGGCGGAGTCTTGTTCTTCTTCGGCTGGGGATGATGTGCTTCACCAGGACATGGAGGATTTTCTGTTCAATATGCTTGGAGAGGGGTTCAAGCTCGATAGGGATGTGATTCGAGATGTTCTTGATAGTTGCGGATATGATATGGAAAAGAGCATGGAAAATCTGATCAGTTTGTCAACGTCAGCCTCGGATGAAAGAAACGAAGTCATCTCTAACTCCAATGATAAGTCTGCAGGCTCAGATCTGAGATTGGAAGAATCTTCTCAGAGAAAGCACATAAACTCCACTGAAGGTAATGGAGACAGGGCTTCAAATACAAATGAAGTAGAATCTACTGGAAAAAGAAAAGAAGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771

Protein Analysis

262

Amino Acids

27.84

Weight (kDa)

4.83

Isoelectric Point (pI)

34.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 242, 350
AccB1I GGYRCC 1 cut(s) 23
AccB7I CCANNNNNTGG 1 cut(s) 481
AccIII TCCGGA 1 cut(s) 412
AciI CCGC 8 cut(s) 141, 144, 165, 174, 177, 203, 434, 561
AcsI RAATTY 1 cut(s) 259
AcuI CTGAAG 1 cut(s) 738
AcyI GRCGYC 3 cut(s) 69, 239, 347
AfiI CCNNNNNNNGG 1 cut(s) 481
AgsI TTSAA 4 cut(s) 19, 499, 520, 741
AhdI GACNNNNNGTC 1 cut(s) 391
AjnI CCWGG 1 cut(s) 474
AloI GAACNNNNNNTCC 2 cut(s) 479, 511
AluBI AGCT 3 cut(s) 8, 420, 523
AluI AGCT 3 cut(s) 8, 420, 523
Alw26I GTCTC 2 cut(s) 143, 723
Aor13HI TCCGGA 1 cut(s) 412
AoxI GGCC 2 cut(s) 120, 138
ApoI RAATTY 1 cut(s) 259
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 224, 464
BaeGI GKGCMC 1 cut(s) 28
BanI GGYRCC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 281
BccI CCATC 2 cut(s) 203, 292
BciT130I CCWGG 1 cut(s) 476
BclI TGATCA 1 cut(s) 594
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 2 cut(s) 143, 723
BfmI CTRYAG 1 cut(s) 660
BglII AGATCT 1 cut(s) 670
BisI GCNGC 4 cut(s) 141, 144, 175, 178
BlsI GCNGC 4 cut(s) 142, 145, 176, 179
Bme1390I CCNGG 2 cut(s) 124, 476
BmeRI GACNNNNNGTC 1 cut(s) 391
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 2 cut(s) 124, 476
BpiI GAAGAC 1 cut(s) 281
BpuEI CTTGAG 2 cut(s) 73, 397
BpuMI CCSGG 1 cut(s) 124
BsaHI GRCGYC 3 cut(s) 69, 239, 347
BsaJI CCNNGG 3 cut(s) 135, 396, 615
BsaWI WCCGGW 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 695, 725
Bsc4I CCNNNNNNNGG 1 cut(s) 481
Bse1I ACTGG 1 cut(s) 769
BseAI TCCGGA 1 cut(s) 412
BseBI CCWGG 1 cut(s) 476
BseDI CCNNGG 3 cut(s) 135, 396, 615
BseGI GGATG 4 cut(s) 211, 466, 538, 625
BseLI CCNNNNNNNGG 1 cut(s) 481
BseMII CTCAG 4 cut(s) 84, 665, 681, 707
BseNI ACTGG 1 cut(s) 769
BseSI GKGCMC 1 cut(s) 28
BseYI CCCAGC 2 cut(s) 420, 455
Bsh1285I CGRYCG 1 cut(s) 290
BshFI GGCC 2 cut(s) 122, 140
BshNI GGYRCC 1 cut(s) 23
BsiEI CGRYCG 1 cut(s) 290
BsiSI CCGG 2 cut(s) 123, 413
BslI CCNNNNNNNGG 1 cut(s) 481
BsmAI GTCTC 2 cut(s) 143, 723
BsnI GGCC 2 cut(s) 122, 140
Bsp1286I GDGCHC 1 cut(s) 28
Bsp13I TCCGGA 1 cut(s) 412
Bsp143I GATC 2 cut(s) 594, 670
BspACI CCGC 8 cut(s) 141, 144, 165, 174, 177, 203, 434, 561
BspANI GGCC 2 cut(s) 122, 140
BspCNI CTCAG 4 cut(s) 85, 666, 680, 706
BspEI TCCGGA 1 cut(s) 412
BspLI GGNNCC 1 cut(s) 25
BspMAI CTGCAG 1 cut(s) 664
BspQI GCTCTTC 1 cut(s) 221
BspT107I GGYRCC 1 cut(s) 23
BsrI ACTGG 1 cut(s) 769
BssECI CCNNGG 3 cut(s) 135, 396, 615
BssMI GATC 2 cut(s) 594, 670
BssNI GRCGYC 3 cut(s) 69, 239, 347
BssT1I CCWWGG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 476
Bst6I CTCTTC 3 cut(s) 177, 221, 774
BstACI GRCGYC 3 cut(s) 69, 239, 347
BstC8I GCNNGC 1 cut(s) 664
BstDEI CTNAG 4 cut(s) 93, 667, 674, 693
BstF5I GGATG 4 cut(s) 211, 466, 538, 625
BstKTI GATC 2 cut(s) 597, 673
BstMAI GTCTC 2 cut(s) 143, 723
BstMBI GATC 2 cut(s) 594, 670
BstMCI CGRYCG 1 cut(s) 290
BstMWI GCNNNNNNNGC 2 cut(s) 140, 164
BstNI CCWGG 1 cut(s) 476
BstSCI CCNGG 2 cut(s) 122, 474
BstSFI CTRYAG 1 cut(s) 660
BstSLI GKGCMC 1 cut(s) 28
BstV2I GAAGAC 1 cut(s) 281
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
BsuRI GGCC 2 cut(s) 122, 140
BtsCI GGATG 4 cut(s) 211, 466, 538, 625
BtsIMutI CAGTG 2 cut(s) 90, 714
Cac8I GCNNGC 1 cut(s) 664
CseI GACGC 1 cut(s) 77
CviAII CATG 2 cut(s) 481, 583
DdeI CTNAG 4 cut(s) 93, 667, 674, 693
DpnI GATC 2 cut(s) 596, 672
DpnII GATC 2 cut(s) 594, 670
DriI GACNNNNNGTC 1 cut(s) 391
Eam1104I CTCTTC 3 cut(s) 177, 221, 774
Eam1105I GACNNNNNGTC 1 cut(s) 391
EarI CTCTTC 3 cut(s) 177, 221, 774
EciI GGCGGA 2 cut(s) 154, 449
Eco130I CCWWGG 1 cut(s) 396
Eco57I CTGAAG 1 cut(s) 738
EcoRI GAATTC 1 cut(s) 259
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 396
FaeI CATG 2 cut(s) 484, 586
FaiI YATR 8 cut(s) 83, 333, 482, 503, 567, 572, 584, 707
FatI CATG 2 cut(s) 480, 582
FbaI TGATCA 1 cut(s) 594
Fnu4HI GCNGC 4 cut(s) 141, 144, 175, 178
FokI GGATG 4 cut(s) 218, 473, 545, 632
Fsp4HI GCNGC 4 cut(s) 141, 144, 175, 178
GluI GCNGC 4 cut(s) 141, 144, 175, 178
GsaI CCCAGC 2 cut(s) 424, 459
HaeIII GGCC 2 cut(s) 122, 140
HapII CCGG 2 cut(s) 123, 413
HgaI GACGC 1 cut(s) 77
Hin1I GRCGYC 3 cut(s) 69, 239, 347
Hin1II CATG 2 cut(s) 484, 586
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HindIII AAGCTT 1 cut(s) 6
HinfI GANTC 9 cut(s) 33, 151, 185, 386, 409, 437, 538, 686, 758
HpaII CCGG 2 cut(s) 123, 413
HphI GGTGA 2 cut(s) 224, 464
Hpy166II GTNNAC 2 cut(s) 385, 606
Hpy188I TCNGA 6 cut(s) 244, 594, 619, 670, 675, 696
Hpy188III TCNNGA 4 cut(s) 192, 413, 542, 551
Hpy8I GTNNAC 2 cut(s) 385, 606
Hpy99I CGWCG 9 cut(s) 41, 44, 71, 193, 241, 244, 349, 352, 355
HpyAV CCTTC 2 cut(s) 364, 713
HpyCH4IV ACGT 3 cut(s) 239, 347, 608
HpyCH4V TGCA 2 cut(s) 117, 662
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 164
HpyF3I CTNAG 4 cut(s) 93, 667, 674, 693
HpySE526I ACGT 3 cut(s) 239, 347, 608
Hsp92I GRCGYC 3 cut(s) 69, 239, 347
Hsp92II CATG 2 cut(s) 484, 586
Kpn2I TCCGGA 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 594
Kzo9I GATC 2 cut(s) 594, 670
LguI GCTCTTC 1 cut(s) 221
MaeII ACGT 3 cut(s) 239, 347, 608
MalI GATC 2 cut(s) 596, 672
MboI GATC 2 cut(s) 594, 670
MboII GAAGA 9 cut(s) 139, 194, 235, 238, 286, 438, 441, 681, 695
MflI RGATCY 1 cut(s) 670
MhlI GDGCHC 1 cut(s) 28
MluCI AATT 1 cut(s) 259
MlyI GAGTC 4 cut(s) 145, 194, 380, 446
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 8 cut(s) 48, 88, 130, 258, 359, 478, 505, 625
MroI TCCGGA 1 cut(s) 412
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 2 cut(s) 123, 413
MspR9I CCNGG 2 cut(s) 124, 476
MvaI CCWGG 1 cut(s) 476
MwoI GCNNNNNNNGC 2 cut(s) 140, 164
NciI CCSGG 1 cut(s) 124
NdeII GATC 2 cut(s) 594, 670
NlaIII CATG 2 cut(s) 484, 586
NlaIV GGNNCC 1 cut(s) 25
NmeAIII GCCGAG 2 cut(s) 160, 172
PciSI GCTCTTC 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 269
PfeI GAWTC 5 cut(s) 33, 409, 538, 686, 758
PflFI GACNNNGTC 1 cut(s) 349
PflMI CCANNNNNTGG 1 cut(s) 481
PkrI GCNGC 4 cut(s) 142, 145, 176, 179
PleI GAGTC 4 cut(s) 145, 193, 380, 445
PpsI GAGTC 4 cut(s) 145, 193, 380, 445
Psp6I CCWGG 1 cut(s) 474
PspFI CCCAGC 2 cut(s) 420, 455
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 1 cut(s) 25
PstI CTGCAG 1 cut(s) 664
PsuI RGATCY 1 cut(s) 670
PsyI GACNNNGTC 1 cut(s) 349
SapI GCTCTTC 1 cut(s) 221
SatI GCNGC 4 cut(s) 141, 144, 175, 178
Sau3AI GATC 2 cut(s) 594, 670
SchI GAGTC 4 cut(s) 145, 194, 380, 446
ScrFI CCNGG 2 cut(s) 124, 476
SduI GDGCHC 1 cut(s) 28
SetI ASST 8 cut(s) 10, 242, 350, 402, 422, 525, 611, 724
SfcI CTRYAG 1 cut(s) 660
SmlI CTYRAG 2 cut(s) 52, 376
SmoI CTYRAG 2 cut(s) 52, 376
Sse9I AATT 1 cut(s) 259
SsiI CCGC 8 cut(s) 141, 144, 165, 174, 177, 203, 434, 561
StyD4I CCNGG 2 cut(s) 122, 474
StyI CCWWGG 1 cut(s) 396
TaiI ACGT 3 cut(s) 242, 350, 611
TaqI TCGA 6 cut(s) 36, 66, 263, 296, 525, 541
TaqII GACCGA 1 cut(s) 276
TasI AATT 1 cut(s) 259
TauI GCSGC 4 cut(s) 143, 146, 177, 180
TfiI GAWTC 5 cut(s) 33, 409, 538, 686, 758
TscAI CASTG 2 cut(s) 97, 721
TspDTI ATGAA 2 cut(s) 636, 765
TspRI CASTG 2 cut(s) 97, 721
Tth111I GACNNNGTC 1 cut(s) 349
Van91I CCANNNNNTGG 1 cut(s) 481
XapI RAATTY 1 cut(s) 259
ZraI GACGTC 2 cut(s) 240, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.