Rh5DG058600

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007493
Physical Location & Seq
Forward (+)
4936019 .. 4941446
5428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG058600.1

Sequence Viewer

Length: 1116 bp
ATGGAAGCTTCTGGTTTCAATGGCGCCCCCAGATTCGACGACGAACAGGGCTTGAGGGTTTTGCTCGACGCCTTTGGCCCTGTGTTTTCCATTGAGGAAATCGCTTCGGCTTATTGCAAGGCCGGGAAGAACGCCGAGGCCGCCGCCGAGACTCTGACTCTCTCGGCCGCCAGCGGCGGCGAAGAATCGTCGGGAGTGGTATCGGATGATGGTGGGATTGGGAAGAAGGACAAAACGACGTCGGATAATGGGAGTGGGAATTCGAGGGGTGCGAAGACGAAGTATCGGTCGGTTTCAGTGGGGAGTGTTTCTGGTGTTATTGGGAAGCATTATGGGAACAAAACGACGTCGTCGGGTGGTAATGGAGGGAAGGCCTTGAGAGTGGACACGAAGTCCAAGGTGTTGCCGATTCCGGAGAGCTGGGTGGAAAAGGCCGAGCCTTGTTCTTCTTCGGCTGGGGATGATGTGCTTCACCAGGACATGGAGGATTTTCTGTTCAATATGCTTGGAGAGGGGTTCAAGCTCGATAGGGATGTGATTCGAGACGTTCTTGATAGTTGCGGATATGATATGGAAAAGAGCATGGAAAATCTGATCAGTTTGTCAACGTCAGCCTCGGATGAAAGAAACGAAGTCATCTCTAACTCCAATGATAAGTCTGCAGGCTCATATCTGAAATTGGAAGAGTCTTCTCAGAGAAAGCACATAAACTCCACTGAAGGTAATGGAGACAGGGCTTCAAATACAAATGAAGTAGAATCTACTGGAAAAGAAAAGAAGAGATTTGATCTACAGAAGGAACTTTTGGCTGCTCTCTTCAATGCTCCTGAGAGACCCGAGGAGCCGGAGCTACCAAAAACAAGAATAGTAAGATCAACAAAGGGATATGCAGACTACGGCCATTTGGTGCTTGAACCTCCAAAAGATTCCTTTTCAGATCACAAGACACCTGTGGTCTATCAACAGCATCATACTGAGGATGGTATGCACCCTTTTCTCTACACTGGTGTTTCCCTGGTACCCTCGATGGAGCGGAATAGTTGGATCAGTCTGTGTTGTAAAGGAATTCATTCAGTTCCTACTAAATACAAATTAAGCCTAAATATGGATCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771

Protein Analysis

371

Amino Acids

40.15

Weight (kDa)

5.28

Isoelectric Point (pI)

37.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_At5g58720 PF24767 15 - 53 3.9e-06 At5g58720 UBA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 242, 350
Acc65I GGTACC 1 cut(s) 1018
AccB1I GGYRCC 2 cut(s) 23, 1018
AccB7I CCANNNNNTGG 1 cut(s) 481
AccBSI CCGCTC 1 cut(s) 1033
AccIII TCCGGA 1 cut(s) 412
AciI CCGC 7 cut(s) 141, 144, 168, 174, 177, 561, 1033
AclWI GGATC 1 cut(s) 1052
AcoI YGGCCR 2 cut(s) 165, 898
AcsI RAATTY 2 cut(s) 259, 1065
AcuI CTGAAG 1 cut(s) 738
AcyI GRCGYC 4 cut(s) 24, 69, 239, 347
AfaI GTAC 1 cut(s) 1020
AfiI CCNNNNNNNGG 2 cut(s) 481, 1105
AgsI TTSAA 6 cut(s) 19, 499, 520, 741, 820, 914
AhdI GACNNNNNGTC 1 cut(s) 391
AjnI CCWGG 2 cut(s) 474, 1014
AjuI GAANNNNNNNTTGG 2 cut(s) 788, 820
AloI GAACNNNNNNTCC 2 cut(s) 479, 511
AluBI AGCT 4 cut(s) 8, 420, 523, 850
AluI AGCT 4 cut(s) 8, 420, 523, 850
Alw26I GTCTC 4 cut(s) 143, 537, 723, 826
AlwI GGATC 1 cut(s) 1052
Ama87I CYCGRG 1 cut(s) 836
Aor13HI TCCGGA 1 cut(s) 412
AoxI GGCC 7 cut(s) 76, 120, 138, 165, 372, 432, 898
ApeKI GCWGC 1 cut(s) 809
ApoI RAATTY 2 cut(s) 259, 1065
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
Asp700I GAANNNNTTC 1 cut(s) 1069
Asp718I GGTACC 1 cut(s) 1018
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 1 cut(s) 77
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 1 cut(s) 464
AvaI CYCGRG 1 cut(s) 836
BanI GGYRCC 2 cut(s) 23, 1018
BbsI GAAGAC 2 cut(s) 281, 681
BbvI GCAGC 1 cut(s) 796
BccI CCATC 3 cut(s) 203, 974, 1021
BceAI ACGGC 1 cut(s) 913
BciT130I CCWGG 2 cut(s) 476, 1016
BclI TGATCA 1 cut(s) 594
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 4 cut(s) 143, 537, 723, 826
BfmI CTRYAG 2 cut(s) 660, 791
BfoI RGCGCY 1 cut(s) 27
BisI GCNGC 6 cut(s) 141, 144, 168, 175, 178, 810
BlsI GCNGC 6 cut(s) 142, 145, 169, 176, 179, 811
Bme1390I CCNGG 3 cut(s) 124, 476, 1016
BmeRI GACNNNNNGTC 1 cut(s) 391
BmeT110I CYCGRG 1 cut(s) 836
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 3 cut(s) 25, 843, 1020
BmrFI CCNGG 3 cut(s) 124, 476, 1016
BmsI GCATC 1 cut(s) 976
BpiI GAAGAC 2 cut(s) 281, 681
BpuEI CTTGAG 2 cut(s) 73, 397
BpuMI CCSGG 1 cut(s) 124
BsaHI GRCGYC 4 cut(s) 24, 69, 239, 347
BsaI GGTCTC 1 cut(s) 826
BsaJI CCNNGG 5 cut(s) 135, 396, 615, 837, 1014
BsaWI WCCGGW 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 695, 725
Bsc4I CCNNNNNNNGG 2 cut(s) 481, 1105
Bse1I ACTGG 2 cut(s) 769, 1009
BseAI TCCGGA 1 cut(s) 412
BseBI CCWGG 2 cut(s) 476, 1016
BseDI CCNNGG 5 cut(s) 135, 396, 615, 837, 1014
BseGI GGATG 5 cut(s) 211, 466, 538, 625, 985
BseLI CCNNNNNNNGG 2 cut(s) 481, 1105
BseMII CTCAG 3 cut(s) 707, 819, 966
BseNI ACTGG 2 cut(s) 769, 1009
BseRI GAGGAG 1 cut(s) 854
BseX3I CGGCCG 1 cut(s) 165
BseXI GCAGC 1 cut(s) 796
BseYI CCCAGC 2 cut(s) 420, 455
Bsh1285I CGRYCG 2 cut(s) 168, 290
BshFI GGCC 7 cut(s) 78, 122, 140, 167, 374, 434, 900
BshNI GGYRCC 2 cut(s) 23, 1018
BsiEI CGRYCG 2 cut(s) 168, 290
BsiHKCI CYCGRG 1 cut(s) 836
BsiSI CCGG 3 cut(s) 123, 413, 845
BslI CCNNNNNNNGG 2 cut(s) 481, 1105
BsmAI GTCTC 4 cut(s) 143, 537, 723, 826
BsmBI CGTCTC 1 cut(s) 537
BsnI GGCC 7 cut(s) 78, 122, 140, 167, 374, 434, 900
Bso31I GGTCTC 1 cut(s) 826
BsoBI CYCGRG 1 cut(s) 836
Bsp13I TCCGGA 1 cut(s) 412
Bsp143I GATC 6 cut(s) 594, 787, 872, 937, 1044, 1108
BspACI CCGC 7 cut(s) 141, 144, 168, 174, 177, 561, 1033
BspANI GGCC 7 cut(s) 78, 122, 140, 167, 374, 434, 900
BspCNI CTCAG 3 cut(s) 706, 820, 967
BspEI TCCGGA 1 cut(s) 412
BspLI GGNNCC 3 cut(s) 25, 843, 1020
BspMAI CTGCAG 1 cut(s) 664
BspPI GGATC 1 cut(s) 1052
BspT107I GGYRCC 2 cut(s) 23, 1018
BspTNI GGTCTC 1 cut(s) 826
BsrBI CCGCTC 1 cut(s) 1033
BsrI ACTGG 2 cut(s) 769, 1009
BssECI CCNNGG 5 cut(s) 135, 396, 615, 837, 1014
BssMI GATC 6 cut(s) 594, 787, 872, 937, 1044, 1108
BssNI GRCGYC 4 cut(s) 24, 69, 239, 347
BssT1I CCWWGG 1 cut(s) 396
Bst2UI CCWGG 2 cut(s) 476, 1016
Bst6I CTCTTC 3 cut(s) 678, 773, 821
BstACI GRCGYC 4 cut(s) 24, 69, 239, 347
BstC8I GCNNGC 2 cut(s) 172, 664
BstDEI CTNAG 3 cut(s) 693, 828, 975
BstF5I GGATG 5 cut(s) 211, 466, 538, 625, 985
BstH2I RGCGCY 1 cut(s) 27
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 6 cut(s) 597, 790, 875, 940, 1047, 1111
BstMAI GTCTC 4 cut(s) 143, 537, 723, 826
BstMBI GATC 6 cut(s) 594, 787, 872, 937, 1044, 1108
BstMCI CGRYCG 2 cut(s) 168, 290
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstNI CCWGG 2 cut(s) 476, 1016
BstSCI CCNGG 3 cut(s) 122, 474, 1014
BstSFI CTRYAG 2 cut(s) 660, 791
BstV1I GCAGC 1 cut(s) 796
BstV2I GAAGAC 2 cut(s) 281, 681
BstX2I RGATCY 1 cut(s) 1108
BstYI RGATCY 1 cut(s) 1108
BstZI CGGCCG 1 cut(s) 165
BsuRI GGCC 7 cut(s) 78, 122, 140, 167, 374, 434, 900
BtsCI GGATG 5 cut(s) 211, 466, 538, 625, 985
BtsIMutI CAGTG 3 cut(s) 303, 714, 1002
Cac8I GCNNGC 2 cut(s) 172, 664
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 1 cut(s) 77
CseI GACGC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 1019
CviAII CATG 2 cut(s) 481, 583
CviQI GTAC 1 cut(s) 1019
DdeI CTNAG 3 cut(s) 693, 828, 975
DinI GGCGCC 1 cut(s) 25
DpnI GATC 6 cut(s) 596, 789, 874, 939, 1046, 1110
DpnII GATC 6 cut(s) 594, 787, 872, 937, 1044, 1108
DriI GACNNNNNGTC 1 cut(s) 391
EaeI YGGCCR 2 cut(s) 165, 898
EagI CGGCCG 1 cut(s) 165
Eam1104I CTCTTC 3 cut(s) 678, 773, 821
Eam1105I GACNNNNNGTC 1 cut(s) 391
EarI CTCTTC 3 cut(s) 678, 773, 821
EclXI CGGCCG 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 396
Eco147I AGGCCT 1 cut(s) 374
Eco31I GGTCTC 1 cut(s) 826
Eco52I CGGCCG 1 cut(s) 165
Eco57I CTGAAG 1 cut(s) 738
Eco88I CYCGRG 1 cut(s) 836
EcoRI GAATTC 2 cut(s) 259, 1065
EcoRII CCWGG 2 cut(s) 474, 1014
EcoT14I CCWWGG 1 cut(s) 396
EgeI GGCGCC 1 cut(s) 25
EheI GGCGCC 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 537
FaeI CATG 2 cut(s) 484, 586
FatI CATG 2 cut(s) 480, 582
FbaI TGATCA 1 cut(s) 594
Fnu4HI GCNGC 6 cut(s) 141, 144, 168, 175, 178, 810
FokI GGATG 5 cut(s) 218, 473, 545, 632, 992
Fsp4HI GCNGC 6 cut(s) 141, 144, 168, 175, 178, 810
GlaI GCGC 1 cut(s) 25
GluI GCNGC 6 cut(s) 141, 144, 168, 175, 178, 810
GsaI CCCAGC 2 cut(s) 424, 459
HaeII RGCGCY 1 cut(s) 27
HaeIII GGCC 7 cut(s) 78, 122, 140, 167, 374, 434, 900
HapII CCGG 3 cut(s) 123, 413, 845
HgaI GACGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 26
Hin1I GRCGYC 4 cut(s) 24, 69, 239, 347
Hin1II CATG 2 cut(s) 484, 586
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HindIII AAGCTT 1 cut(s) 6
HinfI GANTC 9 cut(s) 33, 151, 157, 185, 409, 538, 686, 758, 926
HpaII CCGG 3 cut(s) 123, 413, 845
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 2 cut(s) 385, 606
Hpy188I TCNGA 8 cut(s) 156, 205, 244, 594, 619, 675, 696, 937
Hpy188III TCNNGA 5 cut(s) 192, 413, 542, 551, 827
Hpy8I GTNNAC 2 cut(s) 385, 606
Hpy99I CGWCG 9 cut(s) 41, 44, 71, 193, 241, 244, 349, 352, 355
HpyAV CCTTC 4 cut(s) 220, 364, 713, 790
HpyCH4IV ACGT 4 cut(s) 239, 347, 546, 608
HpyCH4V TGCA 4 cut(s) 117, 662, 890, 988
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 3 cut(s) 693, 828, 975
HpySE526I ACGT 4 cut(s) 239, 347, 546, 608
Hsp92I GRCGYC 4 cut(s) 24, 69, 239, 347
Hsp92II CATG 2 cut(s) 484, 586
HspAI GCGC 1 cut(s) 24
KasI GGCGCC 1 cut(s) 23
Kpn2I TCCGGA 1 cut(s) 412
KpnI GGTACC 1 cut(s) 1022
Ksp22I TGATCA 1 cut(s) 594
Kzo9I GATC 6 cut(s) 594, 787, 872, 937, 1044, 1108
LmnI GCTCC 4 cut(s) 829, 841, 847, 1030
Lsp1109I GCAGC 1 cut(s) 796
LweI GCATC 1 cut(s) 976
MaeII ACGT 4 cut(s) 239, 347, 546, 608
MalI GATC 6 cut(s) 596, 789, 874, 939, 1046, 1110
MbiI CCGCTC 1 cut(s) 1033
MboI GATC 6 cut(s) 594, 787, 872, 937, 1044, 1108
MflI RGATCY 1 cut(s) 1108
MluCI AATT 4 cut(s) 259, 677, 1065, 1091
Mly113I GGCGCC 1 cut(s) 24
MlyI GAGTC 3 cut(s) 145, 151, 695
MmeI TCCRAC 2 cut(s) 222, 1022
MroI TCCGGA 1 cut(s) 412
MroXI GAANNNNTTC 1 cut(s) 1069
MseI TTAA 1 cut(s) 1094
MspA1I CMGCKG 1 cut(s) 174
MspI CCGG 3 cut(s) 123, 413, 845
MspR9I CCNGG 3 cut(s) 124, 476, 1016
MvaI CCWGG 2 cut(s) 476, 1016
MwoI GCNNNNNNNGC 1 cut(s) 140
NarI GGCGCC 1 cut(s) 24
NciI CCSGG 1 cut(s) 124
NdeII GATC 6 cut(s) 594, 787, 872, 937, 1044, 1108
NlaIII CATG 2 cut(s) 484, 586
NlaIV GGNNCC 3 cut(s) 25, 843, 1020
NmeAIII GCCGAG 4 cut(s) 143, 160, 172, 460
PceI AGGCCT 1 cut(s) 374
PcsI WCGNNNNNNNCGW 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 1069
PfeI GAWTC 6 cut(s) 33, 185, 409, 538, 758, 926
PflFI GACNNNGTC 1 cut(s) 349
PflMI CCANNNNNTGG 1 cut(s) 481
PkrI GCNGC 6 cut(s) 142, 145, 169, 176, 179, 811
PleI GAGTC 3 cut(s) 145, 151, 694
PluTI GGCGCC 1 cut(s) 27
PpsI GAGTC 3 cut(s) 145, 151, 694
Psp6I CCWGG 2 cut(s) 474, 1014
PspFI CCCAGC 2 cut(s) 420, 455
PspGI CCWGG 2 cut(s) 474, 1014
PspN4I GGNNCC 3 cut(s) 25, 843, 1020
PspPI GGNCC 1 cut(s) 77
PstI CTGCAG 1 cut(s) 664
PsuI RGATCY 1 cut(s) 1108
PsyI GACNNNGTC 1 cut(s) 349
RsaI GTAC 1 cut(s) 1020
RsaNI GTAC 1 cut(s) 1019
SaqAI TTAA 1 cut(s) 1094
SatI GCNGC 6 cut(s) 141, 144, 168, 175, 178, 810
Sau3AI GATC 6 cut(s) 594, 787, 872, 937, 1044, 1108
Sau96I GGNCC 1 cut(s) 77
SchI GAGTC 3 cut(s) 145, 151, 695
ScrFI CCNGG 3 cut(s) 124, 476, 1016
SfaNI GCATC 1 cut(s) 976
SfcI CTRYAG 2 cut(s) 660, 791
SfoI GGCGCC 1 cut(s) 25
SmlI CTYRAG 2 cut(s) 52, 376
SmoI CTYRAG 2 cut(s) 52, 376
Sse9I AATT 4 cut(s) 259, 677, 1065, 1091
SseBI AGGCCT 1 cut(s) 374
SsiI CCGC 7 cut(s) 141, 144, 168, 174, 177, 561, 1033
SspDI GGCGCC 1 cut(s) 23
StuI AGGCCT 1 cut(s) 374
StyD4I CCNGG 3 cut(s) 122, 474, 1014
StyI CCWWGG 1 cut(s) 396
TaiI ACGT 4 cut(s) 242, 350, 549, 611
TaqI TCGA 6 cut(s) 36, 66, 263, 525, 541, 1025
TaqII GACCGA 1 cut(s) 276
TasI AATT 4 cut(s) 259, 677, 1065, 1091
TauI GCSGC 5 cut(s) 143, 146, 170, 177, 180
TfiI GAWTC 6 cut(s) 33, 185, 409, 538, 758, 926
Tru1I TTAA 1 cut(s) 1094
Tru9I TTAA 1 cut(s) 1094
TscAI CASTG 3 cut(s) 303, 721, 1009
TseI GCWGC 1 cut(s) 809
TspDTI ATGAA 3 cut(s) 636, 765, 1058
TspRI CASTG 3 cut(s) 303, 721, 1009
Tth111I GACNNNGTC 1 cut(s) 349
Van91I CCANNNNNTGG 1 cut(s) 481
XapI RAATTY 2 cut(s) 259, 1065
XmnI GAANNNNTTC 1 cut(s) 1069
ZraI GACGTC 2 cut(s) 240, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.