Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0007493

Physical Location & Seq

Genomic Coordinates Forward (+)

4866114 .. 4871305

Gene Structure 5192 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG059000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1227 bp

ATGGAAGCTTCTGGTTTCAATGGCGCCTCCAGATTTGACGACGAACAGGGCTTGAGGGTATTGCTCGACGCTTTTGGCCCGGCGTTTTCCCTTGAAGAAATCGCCTCGGCTTATTGCAAGGCCGGGAAGAACGCCGAGGCCGCCGCCGAGACTCTGGCTCTCTCCGCCACCAGCGCCGGCGAAGAATCGTCGGCAGTGGTATCGGATGATGGTGGGATTGGCAAGAAGGACAAAACGACGTCGGATAATGGGAGTGGGAATTCGAGGGGTGCGAAGACGAAGTATCGGTCGGTTTCAGTGGGGAGTGTTTCTGGTGTTATTGGGAAGCATTATGGGAACCAAACGACGTCGTCGGGAGGTAATGGAGGGAAGGCGTTGAGAGTGGACTCAAAGTCCAAGGTGTTGCCGATTCCGGAGAGCTGGGTGGAAAAGGCGGAGTCTTGTTCTTCTTCAGCTGGGGATGATGTGCTTCACCAGGACATGGAGGATTTTCTGTTCAATATGCTTGGAGAGGGGTTCAAGCTCGATAGGGATGTGATTCGAGACGTTCTTGATAGTTGCGGATATGATATGGAAAAGAGCATGGAAAATCTGATCAGTTTGTCAACGTCAGCCTCGGATGAAAGAAACGAAGTCATCTCTAACTCCAATGATAAGTCTGCAGGCTCATATCTGAAATTGGAAGAATCTTCTCAGAGAAAGCACATAAACTCCACTGAAGGTAATGGAGACAGGGCTTCAAATACAAATGAAGTAGAATCTACTGGAAAAGAAAAGAAGAGATTTGATCTACAGAAGGAAGTTTTGGCTGCTCTCTTCAATGCTCCTGAGAGATCTGAGGAGCCGGAGCTACCAAAAACAAGAATAGTAAGATCAACGAAGGGATATGCAGACTACGGCCATTTGGTGCTTGAACCTCCAAAAGATTCCTTTTCAGATCACAAGACACCTGTGGTCTATCAACAGCATCATACTGAGGATGATGCAGATGATGAAGTTAGGTACCAGGTTCTTCGCAAAAGTGTGAAGGAGTATCGGAGTACGATGAAGGAGTATTACCAGGCAGCTGTAGACGCATTTTCTAAGGGAGATCGTATTCGAGCAGACAAACTTATGGAACAGGGACATTTTTTCCAGAAAAAGGCACGCGAAGCAGATGAGGAGTCAAATAAAATGATCCTTAAACCTAGGTCAGCATCCTTTTTTTGTGTTGTCCAACCCTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1771

Protein Analysis

408

Amino Acids

44.64

Weight (kDa)

5.23

Isoelectric Point (pI)

36.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UBA_At5g58720	PF24767	15 - 53	6.7e-06	At5g58720 UBA-like domain
DUF1771	PF08590	335 - 397	4.7e-14	Domain of unknown function (DUF1771)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007493)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g09380 FvH4_6g17870 FvH4_6g17870 FvH4_6g17870 FvH4_6g17870 FvH4_6g17870 FvH4_6g17870 FvH4_6g17870 FvH4_6g17870
malus_domestica	MD00G1095600.v1.1 MD05G1314000.v1.1
prunus_persica	Prupe.4G045500_v2.0.a1 Prupe.4G045500_v2.0.a1
pyrus_communis	pycom05g29180 pycom10g24770
rosa_chinensis	RchiOBHm_Chr5g0008211
rosa_laevigata	RLG00000031549
rosa_roxburghii	Rroxscaffold_1G00068270
rosa_rugosa	Rorug04G0434300 Rorug04G0434400
rosa_samantha	Rh5AG062700 Rh5BG059000 Rh5CG069600 Rh5DG058600
rosa_wichuraiana	Rw5G005830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	242, 350
Acc65I	GGTACC	1 cut(s)	1002
AccB1I	GGYRCC	2 cut(s)	23, 1002
AccB7I	CCANNNNNTGG	1 cut(s)	481
AccI	GTMKAC	1 cut(s)	1071
AccII	CGCG	1 cut(s)	1149
AccIII	TCCGGA	1 cut(s)	412
AciI	CCGC	5 cut(s)	141, 144, 165, 434, 561
AclWI	GGATC	1 cut(s)	1171
AcoI	YGGCCR	1 cut(s)	898
AcsI	RAATTY	1 cut(s)	259
AcuI	CTGAAG	2 cut(s)	435, 738
AcyI	GRCGYC	3 cut(s)	24, 239, 347
AfaI	GTAC	2 cut(s)	1004, 1042
AfiI	CCNNNNNNNGG	2 cut(s)	481, 1141
AgsI	TTSAA	7 cut(s)	19, 95, 499, 520, 741, 820, 914
AhdI	GACNNNNNGTC	1 cut(s)	391
AjnI	CCWGG	3 cut(s)	474, 1005, 1059
AjuI	GAANNNNNNNTTGG	2 cut(s)	788, 820
AloI	GAACNNNNNNTCC	2 cut(s)	479, 511
AluBI	AGCT	6 cut(s)	8, 420, 455, 523, 850, 1067
AluI	AGCT	6 cut(s)	8, 420, 455, 523, 850, 1067
Alw26I	GTCTC	3 cut(s)	143, 537, 723
AlwI	GGATC	1 cut(s)	1171
Aor13HI	TCCGGA	1 cut(s)	412
AoxI	GGCC	4 cut(s)	76, 120, 138, 898
ApeKI	GCWGC	2 cut(s)	809, 1064
ApoI	RAATTY	1 cut(s)	259
ArsI	GACNNNNNNTTYG	2 cut(s)	272, 304
Asp718I	GGTACC	1 cut(s)	1002
AspA2I	CCTAGG	1 cut(s)	1187
AspLEI	GCGC	2 cut(s)	26, 176
AspS9I	GGNCC	1 cut(s)	77
AsuC2I	CCSGG	2 cut(s)	80, 124
AsuHPI	GGTGA	1 cut(s)	464
AvrII	CCTAGG	1 cut(s)	1187
BanI	GGYRCC	2 cut(s)	23, 1002
BarI	GAAGNNNNNNTAC	2 cut(s)	1040, 1072
BbsI	GAAGAC	1 cut(s)	281
BbvI	GCAGC	2 cut(s)	796, 1076
BccI	CCATC	1 cut(s)	203
BceAI	ACGGC	1 cut(s)	913
BcgI	CGANNNNNNTGC	2 cut(s)	183, 217
BciT130I	CCWGG	3 cut(s)	476, 1007, 1061
BclI	TGATCA	1 cut(s)	594
BcnI	CCSGG	2 cut(s)	80, 124
BcoDI	GTCTC	3 cut(s)	143, 537, 723
BfaI	CTAG	1 cut(s)	1188
BfmI	CTRYAG	3 cut(s)	660, 791, 1068
BfoI	RGCGCY	2 cut(s)	27, 177
BglII	AGATCT	1 cut(s)	833
BisI	GCNGC	4 cut(s)	141, 144, 810, 1065
BlnI	CCTAGG	1 cut(s)	1187
BlsI	GCNGC	4 cut(s)	142, 145, 811, 1066
Bme1390I	CCNGG	5 cut(s)	80, 124, 476, 1007, 1061
BmeRI	GACNNNNNGTC	1 cut(s)	391
BmgT120I	GGNCC	1 cut(s)	77
BmiI	GGNNCC	4 cut(s)	25, 338, 843, 1004
BmrFI	CCNGG	5 cut(s)	80, 124, 476, 1007, 1061
BmsI	GCATC	3 cut(s)	973, 976, 1205
BpiI	GAAGAC	1 cut(s)	281
BpmI	CTGGAG	1 cut(s)	13
BpuEI	CTTGAG	1 cut(s)	73
BpuMI	CCSGG	2 cut(s)	80, 124
BsaHI	GRCGYC	3 cut(s)	24, 239, 347
BsaJI	CCNNGG	5 cut(s)	105, 135, 396, 615, 1187
BsaWI	WCCGGW	1 cut(s)	412
BsaXI	ACNNNNNCTCC	2 cut(s)	695, 725
Bsc4I	CCNNNNNNNGG	2 cut(s)	481, 1141
Bse118I	RCCGGY	1 cut(s)	176
Bse1I	ACTGG	1 cut(s)	769
BseAI	TCCGGA	1 cut(s)	412
BseBI	CCWGG	3 cut(s)	476, 1007, 1061
BseDI	CCNNGG	5 cut(s)	105, 135, 396, 615, 1187
BseGI	GGATG	6 cut(s)	211, 466, 538, 625, 985, 1196
BseLI	CCNNNNNNNGG	2 cut(s)	481, 1141
BseMII	CTCAG	4 cut(s)	707, 819, 828, 966
BseNI	ACTGG	1 cut(s)	769
BseRI	GAGGAG	2 cut(s)	854, 1175
BseXI	GCAGC	2 cut(s)	796, 1076
BseYI	CCCAGC	2 cut(s)	420, 455
Bsh1236I	CGCG	1 cut(s)	1149
Bsh1285I	CGRYCG	1 cut(s)	290
BshFI	GGCC	4 cut(s)	78, 122, 140, 900
BshNI	GGYRCC	2 cut(s)	23, 1002
BsiEI	CGRYCG	1 cut(s)	290
BsiSI	CCGG	5 cut(s)	80, 123, 177, 413, 845
BslFI	GGGAC	1 cut(s)	1137
BslI	CCNNNNNNNGG	2 cut(s)	481, 1141
BsmAI	GTCTC	3 cut(s)	143, 537, 723
BsmBI	CGTCTC	1 cut(s)	537
BsmFI	GGGAC	1 cut(s)	1137
BsnI	GGCC	4 cut(s)	78, 122, 140, 900
Bsp13I	TCCGGA	1 cut(s)	412
Bsp143I	GATC	7 cut(s)	594, 787, 833, 872, 937, 1090, 1176
BspACI	CCGC	5 cut(s)	141, 144, 165, 434, 561
BspANI	GGCC	4 cut(s)	78, 122, 140, 900
BspCNI	CTCAG	4 cut(s)	706, 820, 829, 967
BspEI	TCCGGA	1 cut(s)	412
BspFNI	CGCG	1 cut(s)	1149
BspLI	GGNNCC	4 cut(s)	25, 338, 843, 1004
BspMAI	CTGCAG	1 cut(s)	664
BspPI	GGATC	1 cut(s)	1171
BspT107I	GGYRCC	2 cut(s)	23, 1002
BsrFI	RCCGGY	1 cut(s)	176
BsrI	ACTGG	1 cut(s)	769
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	5 cut(s)	105, 135, 396, 615, 1187
BssMI	GATC	7 cut(s)	594, 787, 833, 872, 937, 1090, 1176
BssNI	GRCGYC	3 cut(s)	24, 239, 347
BssT1I	CCWWGG	2 cut(s)	396, 1187
Bst2UI	CCWGG	3 cut(s)	476, 1007, 1061
Bst6I	CTCTTC	2 cut(s)	773, 821
BstACI	GRCGYC	3 cut(s)	24, 239, 347
BstC8I	GCNNGC	3 cut(s)	178, 664, 1147
BstDEI	CTNAG	6 cut(s)	693, 828, 837, 975, 1083, 1224
BstF5I	GGATG	6 cut(s)	211, 466, 538, 625, 985, 1196
BstFNI	CGCG	1 cut(s)	1149
BstH2I	RGCGCY	2 cut(s)	27, 177
BstHHI	GCGC	2 cut(s)	26, 176
BstKTI	GATC	7 cut(s)	597, 790, 836, 875, 940, 1093, 1179
BstMAI	GTCTC	3 cut(s)	143, 537, 723
BstMBI	GATC	7 cut(s)	594, 787, 833, 872, 937, 1090, 1176
BstMCI	CGRYCG	1 cut(s)	290
BstMWI	GCNNNNNNNGC	5 cut(s)	140, 164, 173, 1073, 1151
BstNI	CCWGG	3 cut(s)	476, 1007, 1061
BstSCI	CCNGG	5 cut(s)	78, 122, 474, 1005, 1059
BstSFI	CTRYAG	3 cut(s)	660, 791, 1068
BstUI	CGCG	1 cut(s)	1149
BstV1I	GCAGC	2 cut(s)	796, 1076
BstV2I	GAAGAC	1 cut(s)	281
BstX2I	RGATCY	1 cut(s)	833
BstYI	RGATCY	1 cut(s)	833
BsuRI	GGCC	4 cut(s)	78, 122, 140, 900
BtsCI	GGATG	6 cut(s)	211, 466, 538, 625, 985, 1196
BtsI	GCAGTG	1 cut(s)	201
BtsIMutI	CAGTG	3 cut(s)	201, 303, 714
Cac8I	GCNNGC	3 cut(s)	178, 664, 1147
CfoI	GCGC	2 cut(s)	26, 176
Cfr10I	RCCGGY	1 cut(s)	176
Cfr13I	GGNCC	1 cut(s)	77
CseI	GACGC	2 cut(s)	77, 1082
CsiI	ACCWGGT	1 cut(s)	1005
Csp6I	GTAC	2 cut(s)	1003, 1041
CviAII	CATG	2 cut(s)	481, 583
CviQI	GTAC	2 cut(s)	1003, 1041
DdeI	CTNAG	6 cut(s)	693, 828, 837, 975, 1083, 1224
DinI	GGCGCC	1 cut(s)	25
DpnI	GATC	7 cut(s)	596, 789, 835, 874, 939, 1092, 1178
DpnII	GATC	7 cut(s)	594, 787, 833, 872, 937, 1090, 1176
DriI	GACNNNNNGTC	1 cut(s)	391
EaeI	YGGCCR	1 cut(s)	898
Eam1104I	CTCTTC	2 cut(s)	773, 821
Eam1105I	GACNNNNNGTC	1 cut(s)	391
EarI	CTCTTC	2 cut(s)	773, 821
EciI	GGCGGA	2 cut(s)	154, 449
Eco130I	CCWWGG	2 cut(s)	396, 1187
Eco57I	CTGAAG	2 cut(s)	435, 738
EcoRI	GAATTC	1 cut(s)	259
EcoRII	CCWGG	3 cut(s)	474, 1005, 1059
EcoT14I	CCWWGG	2 cut(s)	396, 1187
EgeI	GGCGCC	1 cut(s)	25
EheI	GGCGCC	1 cut(s)	25
ErhI	CCWWGG	2 cut(s)	396, 1187
Esp3I	CGTCTC	1 cut(s)	537
FaeI	CATG	2 cut(s)	484, 586
FaqI	GGGAC	1 cut(s)	1137
FatI	CATG	2 cut(s)	480, 582
FbaI	TGATCA	1 cut(s)	594
FblI	GTMKAC	1 cut(s)	1071
Fnu4HI	GCNGC	4 cut(s)	141, 144, 810, 1065
FokI	GGATG	6 cut(s)	218, 473, 545, 632, 992, 1183
Fsp4HI	GCNGC	4 cut(s)	141, 144, 810, 1065
FspBI	CTAG	1 cut(s)	1188
GlaI	GCGC	2 cut(s)	25, 175
GluI	GCNGC	4 cut(s)	141, 144, 810, 1065
GsaI	CCCAGC	2 cut(s)	424, 459
GsuI	CTGGAG	1 cut(s)	13
HaeII	RGCGCY	2 cut(s)	27, 177
HaeIII	GGCC	4 cut(s)	78, 122, 140, 900
HapII	CCGG	5 cut(s)	80, 123, 177, 413, 845
HgaI	GACGC	2 cut(s)	77, 1082
HhaI	GCGC	2 cut(s)	26, 176
Hin1I	GRCGYC	3 cut(s)	24, 239, 347
Hin1II	CATG	2 cut(s)	484, 586
Hin6I	GCGC	2 cut(s)	24, 174
HinP1I	GCGC	2 cut(s)	24, 174
HincII	GTYRAC	1 cut(s)	606
HindII	GTYRAC	1 cut(s)	606
HindIII	AAGCTT	1 cut(s)	6
HpaII	CCGG	5 cut(s)	80, 123, 177, 413, 845
HphI	GGTGA	1 cut(s)	464
Hpy166II	GTNNAC	3 cut(s)	385, 606, 1072
Hpy188I	TCNGA	9 cut(s)	205, 244, 594, 619, 675, 696, 838, 937, 1038
Hpy188III	TCNNGA	7 cut(s)	30, 354, 413, 542, 551, 827, 1135
Hpy8I	GTNNAC	3 cut(s)	385, 606, 1072
Hpy99I	CGWCG	8 cut(s)	44, 71, 193, 241, 244, 349, 352, 355
HpyAV	CCTTC	7 cut(s)	220, 364, 713, 790, 874, 1021, 1042
HpyCH4IV	ACGT	4 cut(s)	239, 347, 546, 608
HpyCH4V	TGCA	4 cut(s)	117, 662, 890, 986
HpyF10VI	GCNNNNNNNGC	5 cut(s)	140, 164, 173, 1073, 1151
HpyF3I	CTNAG	6 cut(s)	693, 828, 837, 975, 1083, 1224
HpySE526I	ACGT	4 cut(s)	239, 347, 546, 608
Hsp92I	GRCGYC	3 cut(s)	24, 239, 347
Hsp92II	CATG	2 cut(s)	484, 586
HspAI	GCGC	2 cut(s)	24, 174
KasI	GGCGCC	1 cut(s)	23
Kpn2I	TCCGGA	1 cut(s)	412
KpnI	GGTACC	1 cut(s)	1006
KroI	GCCGGC	1 cut(s)	176
KroNI	GCCGGC	1 cut(s)	178
Ksp22I	TGATCA	1 cut(s)	594
Kzo9I	GATC	7 cut(s)	594, 787, 833, 872, 937, 1090, 1176
LmnI	GCTCC	3 cut(s)	829, 841, 847
Lsp1109I	GCAGC	2 cut(s)	796, 1076
LweI	GCATC	3 cut(s)	973, 976, 1205
MabI	ACCWGGT	1 cut(s)	1005
MaeI	CTAG	1 cut(s)	1188
MaeII	ACGT	4 cut(s)	239, 347, 546, 608
MalI	GATC	7 cut(s)	596, 789, 835, 874, 939, 1092, 1178
MboI	GATC	7 cut(s)	594, 787, 833, 872, 937, 1090, 1176
MflI	RGATCY	1 cut(s)	833
MluCI	AATT	2 cut(s)	259, 677
Mly113I	GGCGCC	1 cut(s)	24
MlyI	GAGTC	4 cut(s)	145, 380, 446, 1172
MmeI	TCCRAC	1 cut(s)	222
MreI	CGCCGGCG	1 cut(s)	176
MroI	TCCGGA	1 cut(s)	412
MroNI	GCCGGC	1 cut(s)	176
MseI	TTAA	1 cut(s)	1182
MspA1I	CMGCKG	2 cut(s)	455, 1067
MspI	CCGG	5 cut(s)	80, 123, 177, 413, 845
MspR9I	CCNGG	5 cut(s)	80, 124, 476, 1007, 1061
MvaI	CCWGG	3 cut(s)	476, 1007, 1061
MvnI	CGCG	1 cut(s)	1149
MwoI	GCNNNNNNNGC	5 cut(s)	140, 164, 173, 1073, 1151
NaeI	GCCGGC	1 cut(s)	178
NarI	GGCGCC	1 cut(s)	24
NciI	CCSGG	2 cut(s)	80, 124
NdeII	GATC	7 cut(s)	594, 787, 833, 872, 937, 1090, 1176
NgoMIV	GCCGGC	1 cut(s)	176
NlaIII	CATG	2 cut(s)	484, 586
NlaIV	GGNNCC	4 cut(s)	25, 338, 843, 1004
NmeAIII	GCCGAG	3 cut(s)	86, 160, 172
PcsI	WCGNNNNNNNCGW	1 cut(s)	269
PdiI	GCCGGC	1 cut(s)	178
PfeI	GAWTC	6 cut(s)	185, 409, 538, 686, 758, 926
PflFI	GACNNNGTC	1 cut(s)	349
PflMI	CCANNNNNTGG	1 cut(s)	481
PkrI	GCNGC	4 cut(s)	142, 145, 811, 1066
PleI	GAGTC	4 cut(s)	145, 380, 445, 1171
PluTI	GGCGCC	1 cut(s)	27
PpsI	GAGTC	4 cut(s)	145, 380, 445, 1171
Psp6I	CCWGG	3 cut(s)	474, 1005, 1059
PspFI	CCCAGC	2 cut(s)	420, 455
PspGI	CCWGG	3 cut(s)	474, 1005, 1059
PspN4I	GGNNCC	4 cut(s)	25, 338, 843, 1004
PspPI	GGNCC	1 cut(s)	77
PstI	CTGCAG	1 cut(s)	664
PsuI	RGATCY	1 cut(s)	833
PsyI	GACNNNGTC	1 cut(s)	349
PvuII	CAGCTG	2 cut(s)	455, 1067
RsaI	GTAC	2 cut(s)	1004, 1042
RsaNI	GTAC	2 cut(s)	1003, 1041
SaqAI	TTAA	1 cut(s)	1182
SatI	GCNGC	4 cut(s)	141, 144, 810, 1065
Sau3AI	GATC	7 cut(s)	594, 787, 833, 872, 937, 1090, 1176
Sau96I	GGNCC	1 cut(s)	77
SchI	GAGTC	4 cut(s)	145, 380, 446, 1172
ScrFI	CCNGG	5 cut(s)	80, 124, 476, 1007, 1061
SexAI	ACCWGGT	1 cut(s)	1005
SfaNI	GCATC	3 cut(s)	973, 976, 1205
SfcI	CTRYAG	3 cut(s)	660, 791, 1068
SfoI	GGCGCC	1 cut(s)	25
SgrAI	CRCCGGYG	1 cut(s)	176
SmlI	CTYRAG	1 cut(s)	52
SmoI	CTYRAG	1 cut(s)	52
Sse9I	AATT	2 cut(s)	259, 677
SsiI	CCGC	5 cut(s)	141, 144, 165, 434, 561
SspDI	GGCGCC	1 cut(s)	23
SspMI	CTAG	1 cut(s)	1188
StyD4I	CCNGG	5 cut(s)	78, 122, 474, 1005, 1059
StyI	CCWWGG	2 cut(s)	396, 1187
TaiI	ACGT	4 cut(s)	242, 350, 549, 611
TaqI	TCGA	5 cut(s)	66, 263, 525, 541, 1099
TaqII	GACCGA	1 cut(s)	276
TasI	AATT	2 cut(s)	259, 677
TauI	GCSGC	2 cut(s)	143, 146
TfiI	GAWTC	6 cut(s)	185, 409, 538, 686, 758, 926
Tru1I	TTAA	1 cut(s)	1182
Tru9I	TTAA	1 cut(s)	1182
TscAI	CASTG	3 cut(s)	201, 303, 721
TseI	GCWGC	2 cut(s)	809, 1064
TspDTI	ATGAA	4 cut(s)	636, 765, 1008, 1061
TspRI	CASTG	3 cut(s)	201, 303, 721
Tth111I	GACNNNGTC	1 cut(s)	349
Van91I	CCANNNNNTGG	1 cut(s)	481
XapI	RAATTY	1 cut(s)	259
XmaJI	CCTAGG	1 cut(s)	1187
XmiI	GTMKAC	1 cut(s)	1071
XspI	CTAG	1 cut(s)	1188
ZraI	GACGTC	2 cut(s)	240, 348

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.