FvH4_3g11120

Protein of unknown function (DUF640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014900
Physical Location & Seq
Forward (+)
6547014 .. 6549122
2109 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11120.t1

Sequence Viewer

Length: 753 bp
ATGTCAGCAGCGGCGGCCGCGGCGGCTGCAGCGGTGGTGGTGAGCCGTTCGAGCTCCAGCAACAGCAGCTCGAGCCCTGAGACCTACCACCACCTGCAGCATCACCAAGCGTTCATGGCGGCACCTTCGCTACCGGTGGCGCCTCAGCTGAGCCGTTACGAGTCGCAGAAGAGGAGGGACTGGATCACTTTCGGGCAGTATCTGAAGAACCACCGCCCGCCGCTGACGCTCTCCCGGTGCAGCGGGGCCCACGTGCTGGAGTTTCTGAGGTACTTAGACCAGTTCGGGAAGACCAAGGTCCATGGGAGTGGCTGCCCTTTCTATGGGCACCCTAACCCTCCGGCACCGTGTCCTTGCCCTCTGAAACAGGCGTGGGGGAGCCTGGACGCGCTCATTGGACGGCTGAGGGCGGCGTTTGAGGAGAACGGTGGGCTGCCGGAGACAAACCCTTTCGGCGCACGCGCCGTGAGGCTGTACCTGAGGGAAGTGAGGGACACGCAGGCCAAGGCTAGAGGGATTGCTTATGAGAAGAAGAAGCGAAAGAAGTCACTGCCACCACAAGGAACACAACTGCTGCAGCATGAGCAGCGAGGGCAACGTGGTAATTTTGTTTCAGGTTACGGTGGTGCTGAGGCTGGAGCTGCGTTAGGAGTTGGAGGTGGCTCAGCTCAGCCAAATGGGGTTATGCTTGGTGGAGCTTCGTCTACTGCTGCAAGAGGATCCATGATGCCGCTATCGGTGTTGAACTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF640

Protein Analysis

251

Amino Acids

26.65

Weight (kDa)

9.98

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALOG_dom PF04852 47 - 165 9.2e-65 ALOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014900)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11120
malus_domestica MD05G1264700.v1.1 MD10G1242600.v1.1
prunus_persica Prupe.4G101300_v2.0.a1
pyrus_communis pycom05g24860 pycom10g20310
rosa_chinensis RchiOBHm_Chr5g0018011
rosa_laevigata RLG00000032333
rosa_multiflora Rmu_sc0010280.1_g000005
rosa_roxburghii Rroxscaffold_1G00059600
rosa_rugosa Rorug05G0039600
rosa_samantha Rh5AG132500 Rh5BG130700 Rh5CG143000 Rh5DG132700
rosa_wichuraiana Rw5G011540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 102
Acc36I ACCTGC 1 cut(s) 102
AccB1I GGYRCC 4 cut(s) 121, 139, 327, 343
AccB7I CCANNNNNTGG 1 cut(s) 256
AccI GTMKAC 1 cut(s) 704
AccII CGCG 3 cut(s) 20, 389, 462
AclWI GGATC 3 cut(s) 191, 714, 727
AcoI YGGCCR 1 cut(s) 15
AcuI CTGAAG 1 cut(s) 224
AcvI CACGTG 1 cut(s) 253
AcyI GRCGYC 1 cut(s) 140
AfaI GTAC 2 cut(s) 272, 476
AfiI CCNNNNNNNGG 3 cut(s) 256, 323, 560
AgeI ACCGGT 1 cut(s) 133
AgsI TTSAA 1 cut(s) 745
AjnI CCWGG 1 cut(s) 381
AluBI AGCT 6 cut(s) 54, 69, 148, 641, 668, 698
AluI AGCT 6 cut(s) 54, 69, 148, 641, 668, 698
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 2 cut(s) 74, 434
AlwI GGATC 3 cut(s) 191, 714, 727
AlwNI CAGNNNCTG 1 cut(s) 202
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 3 cut(s) 15, 246, 501
ApaI GGGCCC 1 cut(s) 250
AsiGI ACCGGT 1 cut(s) 133
AspLEI GCGC 4 cut(s) 142, 391, 458, 464
AspS9I GGNCC 3 cut(s) 246, 247, 298
AsuC2I CCSGG 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 52, 95
AvaI CYCGRG 1 cut(s) 70
AvaII GGWCC 1 cut(s) 298
AxyI CCTNAGG 1 cut(s) 479
BaeGI GKGCMC 2 cut(s) 250, 330
BamHI GGATCC 1 cut(s) 719
BanI GGYRCC 4 cut(s) 121, 139, 327, 343
BanII GRGCYC 3 cut(s) 56, 77, 250
BbrPI CACGTG 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 56
BbvCI CCTCAGC 3 cut(s) 144, 404, 630
BceAI ACGGC 4 cut(s) 30, 138, 416, 449
BciT130I CCWGG 1 cut(s) 383
BcnI CCSGG 1 cut(s) 235
BcoDI GTCTC 2 cut(s) 74, 434
BfaI CTAG 1 cut(s) 510
BfmI CTRYAG 3 cut(s) 27, 95, 575
BfoI RGCGCY 1 cut(s) 143
BfuAI ACCTGC 1 cut(s) 102
BglI GCCNNNNNGGC 1 cut(s) 23
BlpI GCTNAGC 3 cut(s) 149, 664, 669
Bme1390I CCNGG 2 cut(s) 235, 383
Bme18I GGWCC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 70
BmgT120I GGNCC 3 cut(s) 246, 247, 298
BmiI GGNNCC 8 cut(s) 123, 141, 247, 248, 329, 345, 380, 721
BmrFI CCNGG 2 cut(s) 235, 383
BmsI GCATC 2 cut(s) 109, 717
BoxI GACNNNNGTC 1 cut(s) 296
BpiI GAAGAC 1 cut(s) 296
BpmI CTGGAG 3 cut(s) 40, 278, 657
Bpu10I CCTNAGC 3 cut(s) 144, 404, 630
Bpu1102I GCTNAGC 3 cut(s) 149, 664, 669
BpuMI CCSGG 1 cut(s) 235
BsaAI YACGTR 1 cut(s) 253
BsaHI GRCGYC 1 cut(s) 140
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 4 cut(s) 18, 294, 301, 504
BsaWI WCCGGW 1 cut(s) 133
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 323, 560
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 2 cut(s) 185, 280
Bse21I CCTNAGG 1 cut(s) 479
BseBI CCWGG 1 cut(s) 383
BseDI CCNNGG 4 cut(s) 18, 294, 301, 504
BseLI CCNNNNNNNGG 3 cut(s) 256, 323, 560
BseMII CTCAG 9 cut(s) 69, 140, 158, 257, 395, 470, 621, 678, 683
BseNI ACTGG 2 cut(s) 185, 280
BseRI GAGGAG 2 cut(s) 187, 434
BseSI GKGCMC 2 cut(s) 250, 330
BseX3I CGGCCG 1 cut(s) 15
BsgI GTGCAG 1 cut(s) 259
Bsh1236I CGCG 3 cut(s) 20, 389, 462
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 3 cut(s) 17, 248, 503
BshNI GGYRCC 4 cut(s) 121, 139, 327, 343
BshTI ACCGGT 1 cut(s) 133
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 4 cut(s) 134, 235, 341, 437
BslFI GGGAC 2 cut(s) 191, 506
BslI CCNNNNNNNGG 3 cut(s) 256, 323, 560
BsmAI GTCTC 2 cut(s) 74, 434
BsmFI GGGAC 2 cut(s) 191, 506
BsnI GGCC 3 cut(s) 17, 248, 503
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 70
Bsp120I GGGCCC 1 cut(s) 246
Bsp1286I GDGCHC 4 cut(s) 56, 77, 250, 330
Bsp143I GATC 2 cut(s) 183, 719
Bsp1720I GCTNAGC 3 cut(s) 149, 664, 669
Bsp19I CCATGG 1 cut(s) 301
BspANI GGCC 3 cut(s) 17, 248, 503
BspCNI CTCAG 9 cut(s) 70, 141, 157, 258, 396, 471, 622, 677, 682
BspFNI CGCG 3 cut(s) 20, 389, 462
BspLI GGNNCC 8 cut(s) 123, 141, 247, 248, 329, 345, 380, 721
BspMAI CTGCAG 3 cut(s) 31, 99, 579
BspMI ACCTGC 1 cut(s) 102
BspPI GGATC 3 cut(s) 191, 714, 727
BspT107I GGYRCC 4 cut(s) 121, 139, 327, 343
BspTNI GGTCTC 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 2 cut(s) 185, 280
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 4 cut(s) 18, 294, 301, 504
BssMI GATC 2 cut(s) 183, 719
BssNI GRCGYC 1 cut(s) 140
BssT1I CCWWGG 3 cut(s) 294, 301, 504
Bst2UI CCWGG 1 cut(s) 383
Bst4CI ACNGT 3 cut(s) 348, 428, 623
Bst6I CTCTTC 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 253
BstC8I GCNNGC 3 cut(s) 218, 460, 501
BstDSI CCRYGG 2 cut(s) 18, 301
BstFNI CGCG 3 cut(s) 20, 389, 462
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 4 cut(s) 142, 391, 458, 464
BstKTI GATC 2 cut(s) 186, 722
BstMAI GTCTC 2 cut(s) 74, 434
BstMBI GATC 2 cut(s) 183, 719
BstMCI CGRYCG 1 cut(s) 18
BstNI CCWGG 1 cut(s) 383
BstPAI GACNNNNGTC 1 cut(s) 296
BstSCI CCNGG 2 cut(s) 233, 381
BstSFI CTRYAG 3 cut(s) 27, 95, 575
BstSLI GKGCMC 2 cut(s) 250, 330
BstUI CGCG 3 cut(s) 20, 389, 462
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 1 cut(s) 719
BstXI CCANNNNNNTGG 1 cut(s) 308
BstYI RGATCY 1 cut(s) 719
BstZI CGGCCG 1 cut(s) 15
Bsu36I CCTNAGG 1 cut(s) 479
BsuRI GGCC 3 cut(s) 17, 248, 503
BtgI CCRYGG 2 cut(s) 18, 301
BtsI GCAGTG 1 cut(s) 548
BtsIMutI CAGTG 1 cut(s) 548
BveI ACCTGC 1 cut(s) 102
Cac8I GCNNGC 3 cut(s) 218, 460, 501
CaiI CAGNNNCTG 1 cut(s) 202
CciNI GCGGCCGC 1 cut(s) 15
CfoI GCGC 4 cut(s) 142, 391, 458, 464
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 3 cut(s) 246, 247, 298
Cfr42I CCGCGG 1 cut(s) 21
CseI GACGC 2 cut(s) 235, 395
Csp6I GTAC 2 cut(s) 271, 475
CspAI ACCGGT 1 cut(s) 133
CviAII CATG 4 cut(s) 115, 302, 581, 724
CviQI GTAC 2 cut(s) 271, 475
DinI GGCGCC 1 cut(s) 141
DpnI GATC 2 cut(s) 185, 721
DpnII GATC 2 cut(s) 183, 719
EaeI YGGCCR 1 cut(s) 15
EagI CGGCCG 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
Ecl136II GAGCTC 1 cut(s) 54
EclXI CGGCCG 1 cut(s) 15
Eco130I CCWWGG 3 cut(s) 294, 301, 504
Eco24I GRGCYC 3 cut(s) 56, 77, 250
Eco31I GGTCTC 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 298
Eco52I CGGCCG 1 cut(s) 15
Eco53kI GAGCTC 1 cut(s) 54
Eco57I CTGAAG 1 cut(s) 224
Eco72I CACGTG 1 cut(s) 253
Eco81I CCTNAGG 1 cut(s) 479
Eco88I CYCGRG 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 54
EcoO109I RGGNCCY 1 cut(s) 246
EcoRII CCWGG 1 cut(s) 381
EcoT14I CCWWGG 3 cut(s) 294, 301, 504
EcoT38I GRGCYC 3 cut(s) 56, 77, 250
EgeI GGCGCC 1 cut(s) 141
EheI GGCGCC 1 cut(s) 141
ErhI CCWWGG 3 cut(s) 294, 301, 504
FaeI CATG 4 cut(s) 118, 305, 584, 727
FaiI YATR 7 cut(s) 116, 303, 324, 525, 582, 686, 725
FaqI GGGAC 2 cut(s) 191, 506
FatI CATG 4 cut(s) 114, 301, 580, 723
FauI CCCGC 2 cut(s) 225, 236
FblI GTMKAC 1 cut(s) 704
FriOI GRGCYC 3 cut(s) 56, 77, 250
FspBI CTAG 1 cut(s) 510
GlaI GCGC 4 cut(s) 141, 390, 457, 463
GsuI CTGGAG 3 cut(s) 40, 278, 657
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 3 cut(s) 17, 248, 503
HapII CCGG 4 cut(s) 134, 235, 341, 437
HgaI GACGC 2 cut(s) 235, 395
HhaI GCGC 4 cut(s) 142, 391, 458, 464
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 4 cut(s) 118, 305, 584, 727
Hin6I GCGC 4 cut(s) 140, 389, 456, 462
HinP1I GCGC 4 cut(s) 140, 389, 456, 462
HinfI GANTC 1 cut(s) 161
HpaII CCGG 4 cut(s) 134, 235, 341, 437
HphI GGTGA 2 cut(s) 52, 95
Hpy166II GTNNAC 1 cut(s) 705
Hpy188I TCNGA 3 cut(s) 204, 267, 363
Hpy188III TCNNGA 1 cut(s) 286
Hpy8I GTNNAC 1 cut(s) 705
HpyAV CCTTC 1 cut(s) 135
HpyCH4III ACNGT 3 cut(s) 348, 428, 623
HpyCH4IV ACGT 2 cut(s) 252, 598
HpyCH4V TGCA 5 cut(s) 29, 97, 240, 577, 713
HpySE526I ACGT 2 cut(s) 252, 598
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 4 cut(s) 118, 305, 584, 727
HspAI GCGC 4 cut(s) 140, 389, 456, 462
KasI GGCGCC 1 cut(s) 139
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 2 cut(s) 183, 719
LmnI GCTCC 4 cut(s) 59, 378, 638, 695
LweI GCATC 2 cut(s) 109, 717
MaeI CTAG 1 cut(s) 510
MaeII ACGT 2 cut(s) 252, 598
MaeIII GTNAC 3 cut(s) 155, 546, 617
MalI GATC 2 cut(s) 185, 721
MboI GATC 2 cut(s) 183, 719
MboII GAAGA 5 cut(s) 181, 217, 301, 541, 544
MflI RGATCY 1 cut(s) 719
MhlI GDGCHC 4 cut(s) 56, 77, 250, 330
MluCI AATT 1 cut(s) 604
Mly113I GGCGCC 1 cut(s) 140
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 634
MslI CAYNNNNRTG 1 cut(s) 306
MspA1I CMGCKG 6 cut(s) 11, 20, 32, 148, 223, 243
MspI CCGG 4 cut(s) 134, 235, 341, 437
MspR9I CCNGG 2 cut(s) 235, 383
MvaI CCWGG 1 cut(s) 383
MvnI CGCG 3 cut(s) 20, 389, 462
NarI GGCGCC 1 cut(s) 140
NciI CCSGG 1 cut(s) 235
NcoI CCATGG 1 cut(s) 301
NdeII GATC 2 cut(s) 183, 719
NlaIII CATG 4 cut(s) 118, 305, 584, 727
NlaIV GGNNCC 8 cut(s) 123, 141, 247, 248, 329, 345, 380, 721
NmuCI GTSAC 1 cut(s) 546
NotI GCGGCCGC 1 cut(s) 15
PaeR7I CTCGAG 1 cut(s) 70
PaqCI CACCTGC 1 cut(s) 102
PflMI CCANNNNNTGG 1 cut(s) 256
PinAI ACCGGT 1 cut(s) 133
PleI GAGTC 1 cut(s) 169
PluTI GGCGCC 1 cut(s) 143
PmaCI CACGTG 1 cut(s) 253
PmlI CACGTG 1 cut(s) 253
PpsI GAGTC 1 cut(s) 169
Ppu21I YACGTR 1 cut(s) 253
PshAI GACNNNNGTC 1 cut(s) 296
Psp124BI GAGCTC 1 cut(s) 56
Psp6I CCWGG 1 cut(s) 381
PspCI CACGTG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 381
PspN4I GGNNCC 8 cut(s) 123, 141, 247, 248, 329, 345, 380, 721
PspOMI GGGCCC 1 cut(s) 246
PspPI GGNCC 3 cut(s) 246, 247, 298
PspXI VCTCGAGB 1 cut(s) 70
PstI CTGCAG 3 cut(s) 31, 99, 579
PstNI CAGNNNCTG 1 cut(s) 202
PsuI RGATCY 1 cut(s) 719
PvuII CAGCTG 1 cut(s) 148
RsaI GTAC 2 cut(s) 272, 476
RsaNI GTAC 2 cut(s) 271, 475
RseI CAYNNNNRTG 1 cut(s) 306
SacI GAGCTC 1 cut(s) 56
SacII CCGCGG 1 cut(s) 21
Sau3AI GATC 2 cut(s) 183, 719
Sau96I GGNCC 3 cut(s) 246, 247, 298
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 2 cut(s) 235, 383
SduI GDGCHC 4 cut(s) 56, 77, 250, 330
SfaNI GCATC 2 cut(s) 109, 717
SfcI CTRYAG 3 cut(s) 27, 95, 575
SfoI GGCGCC 1 cut(s) 141
Sfr274I CTCGAG 1 cut(s) 70
Sfr303I CCGCGG 1 cut(s) 21
SgrBI CCGCGG 1 cut(s) 21
SinI GGWCC 1 cut(s) 298
SlaI CTCGAG 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 70
SmoI CTYRAG 1 cut(s) 70
Sse9I AATT 1 cut(s) 604
SspDI GGCGCC 1 cut(s) 139
SspMI CTAG 1 cut(s) 510
SstI GAGCTC 1 cut(s) 56
StyD4I CCNGG 2 cut(s) 233, 381
StyI CCWWGG 3 cut(s) 294, 301, 504
TaaI ACNGT 3 cut(s) 348, 428, 623
TaiI ACGT 2 cut(s) 255, 601
TaqI TCGA 2 cut(s) 50, 71
TasI AATT 1 cut(s) 604
TauI GCSGC 9 cut(s) 14, 17, 20, 23, 26, 122, 223, 413, 733
TscAI CASTG 1 cut(s) 555
TseFI GTSAC 1 cut(s) 546
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 1 cut(s) 103
TspRI CASTG 1 cut(s) 555
Van91I CCANNNNNTGG 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 704
XspI CTAG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.