Prupe.4G101300_v2.0.a1

Protein of unknown function (DUF640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0014900
Physical Location & Seq
Reverse (-)
5172182 .. 5172961
780 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G101300.1

Sequence Viewer

Length: 780 bp
ATGTCAGCAGCAGCCGCCGCCGCCGCTGCAGCTGCAGTCTGCCGCTCCAGCTCGACCACCAGCAGCTCCAGCGGCGCCGACAACTACCACCACCACCAACAACAACAACACCGACCGTTAGTCACGCCGTCACTAACGGCAGCGCAGCCGGTGCTGAGCAGGTACGAGTCGCAGAAGCGGCGTGACTGGATCACGTTCGGGCAGTATCTCAAGAACCACAAGCCGCCGCTGACGCTGTCGCGGTGCAGCGGGGCCCACGTGCTGGAGTTCCTCCGGTACCTAGACCAGTTCGGGAAGACCAAGGTCCACGCGGACGTTTGTCCGTTCTACGGGCACCCCCACCCTCCCGCGCCCTGCCCCTGCCCGCTGCGCCAGGCGTGGGGCAGCCTCGACGCGCTCATTGGCCGGCTGCGGGCAGCGTTCGAGGAGAACGGCGGACAGCCCGAGACCAACCCGTTTGGAGCGCGGGCGGTGAGGTTGTATCTGAGGGAGGTGAGGGACAGCCAGGCCAAGGCCAGGGGTATTGCCTATGAGAAGAAGAAGAGGAAGAAAGTAGGGGCATCGCAACAACAACAGCAGCAACAGCAACAGCAACAGCAGCAGCAGGATGAGCCGGGGGAGGAGCAGCAGGGGCAACATGGTAATTTCGGTTCATCAGGTTATGGCTATGGTGTTCTCGGTGGGGGAAGTGATGGGTCATCAAATAGGGTTGTGCTTGTTGGGGCTGCTTCTTCCACTGCTGCTAGGGGCTCTATGATCCCTTTGTCAGCGCTAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF640

Protein Analysis

260

Amino Acids

28.02

Weight (kDa)

9.62

Isoelectric Point (pI)

52.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014900)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11120
malus_domestica MD05G1264700.v1.1 MD10G1242600.v1.1
prunus_persica Prupe.4G101300_v2.0.a1
pyrus_communis pycom05g24860 pycom10g20310
rosa_chinensis RchiOBHm_Chr5g0018011
rosa_laevigata RLG00000032333
rosa_multiflora Rmu_sc0010280.1_g000005
rosa_roxburghii Rroxscaffold_1G00059600
rosa_rugosa Rorug05G0039600
rosa_samantha Rh5AG132500 Rh5BG130700 Rh5CG143000 Rh5DG132700
rosa_wichuraiana Rw5G011540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 150
Acc65I GGTACC 1 cut(s) 276
AccB1I GGYRCC 3 cut(s) 74, 276, 333
AccB7I CCANNNNNTGG 1 cut(s) 262
AccBSI CCGCTC 1 cut(s) 45
AccII CGCG 5 cut(s) 241, 311, 350, 395, 466
AclWI GGATC 2 cut(s) 197, 751
AcoI YGGCCR 1 cut(s) 403
AcvI CACGTG 1 cut(s) 259
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 164, 278
AfeI AGCGCT 1 cut(s) 771
AfiI CCNNNNNNNGG 6 cut(s) 262, 329, 379, 412, 511, 516
AhdI GACNNNNNGTC 1 cut(s) 119
AjnI CCWGG 3 cut(s) 372, 504, 515
AluBI AGCT 3 cut(s) 32, 51, 66
AluI AGCT 3 cut(s) 32, 51, 66
Alw26I GTCTC 1 cut(s) 440
AlwI GGATC 2 cut(s) 197, 751
Ama87I CYCGRG 1 cut(s) 443
Aor51HI AGCGCT 1 cut(s) 771
AoxI GGCC 4 cut(s) 252, 403, 507, 513
ApaI GGGCCC 1 cut(s) 256
Asp718I GGTACC 1 cut(s) 276
AspLEI GCGC 7 cut(s) 77, 145, 352, 372, 397, 466, 772
AspS9I GGNCC 3 cut(s) 252, 253, 304
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 2 cut(s) 484, 505
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 1 cut(s) 304
BaeGI GKGCMC 2 cut(s) 256, 336
BanI GGYRCC 3 cut(s) 74, 276, 333
BanII GRGCYC 2 cut(s) 256, 752
BbrPI CACGTG 1 cut(s) 259
BbsI GAAGAC 1 cut(s) 302
BccI CCATC 1 cut(s) 686
BceAI ACGGC 3 cut(s) 112, 153, 448
BciT130I CCWGG 3 cut(s) 374, 506, 517
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 1 cut(s) 440
BfaI CTAG 2 cut(s) 281, 744
BfmI CTRYAG 2 cut(s) 27, 33
BfoI RGCGCY 2 cut(s) 78, 773
BfuAI ACCTGC 1 cut(s) 150
BlpI GCTNAGC 1 cut(s) 155
Bme1390I CCNGG 4 cut(s) 374, 506, 517, 615
Bme18I GGWCC 1 cut(s) 304
BmeRI GACNNNNNGTC 1 cut(s) 119
BmeT110I CYCGRG 1 cut(s) 443
BmgT120I GGNCC 3 cut(s) 252, 253, 304
BmiI GGNNCC 5 cut(s) 76, 253, 254, 278, 335
BmrFI CCNGG 4 cut(s) 374, 506, 517, 615
BmsI GCATC 1 cut(s) 569
BoxI GACNNNNGTC 2 cut(s) 302, 318
BpiI GAAGAC 1 cut(s) 302
BpmI CTGGAG 3 cut(s) 31, 52, 284
Bpu1102I GCTNAGC 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 615
BsaAI YACGTR 1 cut(s) 259
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 4 cut(s) 300, 510, 516, 614
BsaWI WCCGGW 1 cut(s) 273
Bsc4I CCNNNNNNNGG 6 cut(s) 262, 329, 379, 412, 511, 516
Bse118I RCCGGY 2 cut(s) 148, 405
Bse1I ACTGG 2 cut(s) 191, 286
BseBI CCWGG 3 cut(s) 374, 506, 517
BseDI CCNNGG 4 cut(s) 300, 510, 516, 614
BseGI GGATG 1 cut(s) 613
BseLI CCNNNNNNNGG 6 cut(s) 262, 329, 379, 412, 511, 516
BseMII CTCAG 2 cut(s) 146, 476
BseNI ACTGG 2 cut(s) 191, 286
BseRI GAGGAG 2 cut(s) 440, 635
BseSI GKGCMC 2 cut(s) 256, 336
BsgI GTGCAG 1 cut(s) 265
Bsh1236I CGCG 5 cut(s) 241, 311, 350, 395, 466
Bsh1285I CGRYCG 1 cut(s) 116
BshFI GGCC 4 cut(s) 254, 405, 509, 515
BshNI GGYRCC 3 cut(s) 74, 276, 333
BsiEI CGRYCG 1 cut(s) 116
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 4 cut(s) 149, 274, 406, 614
BslFI GGGAC 1 cut(s) 512
BslI CCNNNNNNNGG 6 cut(s) 262, 329, 379, 412, 511, 516
BsmAI GTCTC 1 cut(s) 440
BsmFI GGGAC 1 cut(s) 512
BsnI GGCC 4 cut(s) 254, 405, 509, 515
Bso31I GGTCTC 1 cut(s) 440
BsoBI CYCGRG 1 cut(s) 443
Bsp120I GGGCCC 1 cut(s) 252
Bsp1286I GDGCHC 3 cut(s) 256, 336, 752
Bsp143I GATC 2 cut(s) 189, 756
Bsp1720I GCTNAGC 1 cut(s) 155
BspANI GGCC 4 cut(s) 254, 405, 509, 515
BspCNI CTCAG 2 cut(s) 147, 477
BspFNI CGCG 5 cut(s) 241, 311, 350, 395, 466
BspLI GGNNCC 5 cut(s) 76, 253, 254, 278, 335
BspMAI CTGCAG 2 cut(s) 31, 37
BspMI ACCTGC 1 cut(s) 150
BspPI GGATC 2 cut(s) 197, 751
BspT107I GGYRCC 3 cut(s) 74, 276, 333
BspTNI GGTCTC 1 cut(s) 440
BsrBI CCGCTC 1 cut(s) 45
BsrFI RCCGGY 2 cut(s) 148, 405
BsrI ACTGG 2 cut(s) 191, 286
BssAI RCCGGY 2 cut(s) 148, 405
BssECI CCNNGG 4 cut(s) 300, 510, 516, 614
BssMI GATC 2 cut(s) 189, 756
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 2 cut(s) 300, 510
Bst2UI CCWGG 3 cut(s) 374, 506, 517
Bst4CI ACNGT 1 cut(s) 117
Bst6I CTCTTC 1 cut(s) 536
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 151
BstBAI YACGTR 1 cut(s) 259
BstC8I GCNNGC 4 cut(s) 365, 407, 414, 468
BstDEI CTNAG 2 cut(s) 155, 485
BstF5I GGATG 1 cut(s) 613
BstFNI CGCG 5 cut(s) 241, 311, 350, 395, 466
BstH2I RGCGCY 2 cut(s) 78, 773
BstHHI GCGC 7 cut(s) 77, 145, 352, 372, 397, 466, 772
BstKTI GATC 2 cut(s) 192, 759
BstMAI GTCTC 1 cut(s) 440
BstMBI GATC 2 cut(s) 189, 756
BstMCI CGRYCG 1 cut(s) 116
BstNI CCWGG 3 cut(s) 374, 506, 517
BstPAI GACNNNNGTC 2 cut(s) 302, 318
BstSCI CCNGG 4 cut(s) 372, 504, 515, 613
BstSFI CTRYAG 2 cut(s) 27, 33
BstSLI GKGCMC 2 cut(s) 256, 336
BstUI CGCG 5 cut(s) 241, 311, 350, 395, 466
BstV2I GAAGAC 1 cut(s) 302
BsuRI GGCC 4 cut(s) 254, 405, 509, 515
BtgZI GCGATG 1 cut(s) 546
BtsCI GGATG 1 cut(s) 613
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 1 cut(s) 735
BveI ACCTGC 1 cut(s) 150
Cac8I GCNNGC 4 cut(s) 365, 407, 414, 468
CfoI GCGC 7 cut(s) 77, 145, 352, 372, 397, 466, 772
Cfr10I RCCGGY 2 cut(s) 148, 405
Cfr13I GGNCC 3 cut(s) 252, 253, 304
CseI GACGC 2 cut(s) 241, 401
Csp6I GTAC 2 cut(s) 163, 277
CviAII CATG 1 cut(s) 638
CviQI GTAC 2 cut(s) 163, 277
DdeI CTNAG 2 cut(s) 155, 485
DinI GGCGCC 1 cut(s) 76
DpnI GATC 2 cut(s) 191, 758
DpnII GATC 2 cut(s) 189, 756
DriI GACNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 1 cut(s) 536
Eam1105I GACNNNNNGTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 536
EciI GGCGGA 1 cut(s) 450
Eco130I CCWWGG 2 cut(s) 300, 510
Eco24I GRGCYC 2 cut(s) 256, 752
Eco31I GGTCTC 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 304
Eco47III AGCGCT 1 cut(s) 771
Eco72I CACGTG 1 cut(s) 259
Eco88I CYCGRG 1 cut(s) 443
EcoO109I RGGNCCY 1 cut(s) 252
EcoRII CCWGG 3 cut(s) 372, 504, 515
EcoT14I CCWWGG 2 cut(s) 300, 510
EcoT38I GRGCYC 2 cut(s) 256, 752
EgeI GGCGCC 1 cut(s) 76
EheI GGCGCC 1 cut(s) 76
ErhI CCWWGG 2 cut(s) 300, 510
FaeI CATG 1 cut(s) 641
FaiI YATR 5 cut(s) 531, 639, 663, 669, 755
FaqI GGGAC 1 cut(s) 512
FatI CATG 1 cut(s) 637
FauI CCCGC 5 cut(s) 242, 355, 372, 405, 459
FokI GGATG 1 cut(s) 620
FriOI GRGCYC 2 cut(s) 256, 752
FspBI CTAG 2 cut(s) 281, 744
GlaI GCGC 7 cut(s) 76, 144, 351, 371, 396, 465, 771
GsuI CTGGAG 3 cut(s) 31, 52, 284
HaeII RGCGCY 2 cut(s) 78, 773
HaeIII GGCC 4 cut(s) 254, 405, 509, 515
HapII CCGG 4 cut(s) 149, 274, 406, 614
HgaI GACGC 2 cut(s) 241, 401
HhaI GCGC 7 cut(s) 77, 145, 352, 372, 397, 466, 772
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 1 cut(s) 641
Hin6I GCGC 7 cut(s) 75, 143, 350, 370, 395, 464, 770
HinP1I GCGC 7 cut(s) 75, 143, 350, 370, 395, 464, 770
HinfI GANTC 1 cut(s) 167
HpaII CCGG 4 cut(s) 149, 274, 406, 614
HphI GGTGA 2 cut(s) 484, 505
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 1 cut(s) 486
Hpy188III TCNNGA 2 cut(s) 211, 292
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 1 cut(s) 395
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4IV ACGT 3 cut(s) 194, 258, 315
HpyCH4V TGCA 3 cut(s) 29, 35, 246
HpyF3I CTNAG 2 cut(s) 155, 485
HpySE526I ACGT 3 cut(s) 194, 258, 315
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 1 cut(s) 641
HspAI GCGC 7 cut(s) 75, 143, 350, 370, 395, 464, 770
KasI GGCGCC 1 cut(s) 74
KpnI GGTACC 1 cut(s) 280
KroI GCCGGC 1 cut(s) 405
KroNI GCCGGC 1 cut(s) 407
Kzo9I GATC 2 cut(s) 189, 756
LmnI GCTCC 4 cut(s) 50, 71, 461, 622
LweI GCATC 1 cut(s) 569
MaeI CTAG 2 cut(s) 281, 744
MaeII ACGT 3 cut(s) 194, 258, 315
MaeIII GTNAC 3 cut(s) 121, 129, 182
MalI GATC 2 cut(s) 191, 758
MbiI CCGCTC 1 cut(s) 45
MboI GATC 2 cut(s) 189, 756
MboII GAAGA 6 cut(s) 307, 547, 550, 553, 559, 723
MhlI GDGCHC 3 cut(s) 256, 336, 752
MluCI AATT 1 cut(s) 643
Mly113I GGCGCC 1 cut(s) 75
MlyI GAGTC 1 cut(s) 176
MroNI GCCGGC 1 cut(s) 405
MspA1I CMGCKG 6 cut(s) 26, 32, 72, 229, 249, 367
MspI CCGG 4 cut(s) 149, 274, 406, 614
MspR9I CCNGG 4 cut(s) 374, 506, 517, 615
MvaI CCWGG 3 cut(s) 374, 506, 517
MvnI CGCG 5 cut(s) 241, 311, 350, 395, 466
NaeI GCCGGC 1 cut(s) 407
NarI GGCGCC 1 cut(s) 75
NciI CCSGG 1 cut(s) 615
NdeII GATC 2 cut(s) 189, 756
NgoMIV GCCGGC 1 cut(s) 405
NlaIII CATG 1 cut(s) 641
NlaIV GGNNCC 5 cut(s) 76, 253, 254, 278, 335
NmuCI GTSAC 3 cut(s) 121, 129, 182
PdiI GCCGGC 1 cut(s) 407
PflFI GACNNNGTC 1 cut(s) 235
PflMI CCANNNNNTGG 1 cut(s) 262
PleI GAGTC 1 cut(s) 175
PluTI GGCGCC 1 cut(s) 78
PmaCI CACGTG 1 cut(s) 259
PmlI CACGTG 1 cut(s) 259
PpsI GAGTC 1 cut(s) 175
Ppu21I YACGTR 1 cut(s) 259
PshAI GACNNNNGTC 2 cut(s) 302, 318
Psp6I CCWGG 3 cut(s) 372, 504, 515
PspCI CACGTG 1 cut(s) 259
PspGI CCWGG 3 cut(s) 372, 504, 515
PspN4I GGNNCC 5 cut(s) 76, 253, 254, 278, 335
PspOMI GGGCCC 1 cut(s) 252
PspPI GGNCC 3 cut(s) 252, 253, 304
PsrI GAACNNNNNNTAC 2 cut(s) 634, 666
PstI CTGCAG 2 cut(s) 31, 37
PsyI GACNNNGTC 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 2 cut(s) 164, 278
RsaNI GTAC 2 cut(s) 163, 277
Sau3AI GATC 2 cut(s) 189, 756
Sau96I GGNCC 3 cut(s) 252, 253, 304
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 4 cut(s) 374, 506, 517, 615
SduI GDGCHC 3 cut(s) 256, 336, 752
SfaNI GCATC 1 cut(s) 569
SfcI CTRYAG 2 cut(s) 27, 33
SfoI GGCGCC 1 cut(s) 76
SinI GGWCC 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 1 cut(s) 643
SspDI GGCGCC 1 cut(s) 74
SspMI CTAG 2 cut(s) 281, 744
StyD4I CCNGG 4 cut(s) 372, 504, 515, 613
StyI CCWWGG 2 cut(s) 300, 510
TaaI ACNGT 1 cut(s) 117
TaiI ACGT 3 cut(s) 197, 261, 318
TaqI TCGA 3 cut(s) 53, 390, 423
TasI AATT 1 cut(s) 643
TauI GCSGC 9 cut(s) 17, 20, 23, 26, 45, 75, 181, 226, 229
TscAI CASTG 1 cut(s) 742
TseFI GTSAC 3 cut(s) 121, 129, 182
Tsp45I GTSAC 3 cut(s) 121, 129, 182
TspDTI ATGAA 1 cut(s) 642
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 1 cut(s) 742
Tth111I GACNNNGTC 1 cut(s) 235
Van91I CCANNNNNTGG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 304
XspI CTAG 2 cut(s) 281, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.