pycom05g24860

Protein of unknown function (DUF640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014900
Physical Location & Seq
Forward (+)
26361911 .. 26363284
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g24860.2

Sequence Viewer

Length: 819 bp
ATGTATATAAAGTTACGTTCAAGATGCCCTAGTTTGGAGGATCAGAACTGCACAGTGAGACACTTTGTCCTCATATCATCATACCAATTTAATCCTTTTGAAAGCTATCAAACTTTGTGTACCATGTCAGCAGCAGCCGCAGCGGCGGCGGCGGTGGCAGTGAGTAATTCGAGCTCCACTAACAGCAACTCCATCACCGACAACTACCACCACCAACCGTTATTCACACCGTCCCCGCTAGCGGCCGTGACGCCAGTGCTGAGCAGATACGAGTCCCAGAAGCGGCGGGACTGGATCACGTTCGGGCAGTACCTCAAGAACCACCGCCCGCCACTTAACCTGTCCCGCTGCAGTGGGGCACACGTGCTGGAGTTCCTTAGGTATTTGGATCAATTCGGGAAGACCAAGGTCCACGCGGACGCTTGCCCTTTCTACGGGCACCCTAACCCTCCTGCGCCCTGCCCATGCCCGTTGCGGCAGGCTTGGGGCAGCCTGGACGCGCTCATTGGGCGGCTTCGGGCAGCTTTTGAGGAGAACGGCGGGCACCCCGAGACTAACCCGTTTGGGGCACGGGCAGTGAGGCTGTACCTGAGGGAGGTGAGGGACAGCCAGGCCAGGGCAAGGGGGATTGCTTATGAGAAGAAGAAGAGAAAGAAAGTAGGGCCGTCACAACAGCTGCAGGGGAATGATCAACAACATGATGAGGAGGAGAGGCATGGGCAACATGGTAATTTTGGTTCAGATTATGGGTATAGTGCTGCTGGGGCTTCATCTGCTACTGCCAGGGACTCCATGATGCCCTTATCTGTGCTAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF640

Protein Analysis

273

Amino Acids

30.23

Weight (kDa)

9.17

Isoelectric Point (pI)

50.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014900)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11120
malus_domestica MD05G1264700.v1.1 MD10G1242600.v1.1
prunus_persica Prupe.4G101300_v2.0.a1
pyrus_communis pycom05g24860 pycom10g20310
rosa_chinensis RchiOBHm_Chr5g0018011
rosa_laevigata RLG00000032333
rosa_multiflora Rmu_sc0010280.1_g000005
rosa_roxburghii Rroxscaffold_1G00059600
rosa_rugosa Rorug05G0039600
rosa_samantha Rh5AG132500 Rh5BG130700 Rh5CG143000 Rh5DG132700
rosa_wichuraiana Rw5G011540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 438, 543
AccII CGCG 2 cut(s) 416, 500
AclWI GGATC 3 cut(s) 48, 302, 396
AcoI YGGCCR 1 cut(s) 243
AcvI CACGTG 1 cut(s) 364
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 3 cut(s) 121, 311, 587
AfiI CCNNNNNNNGG 8 cut(s) 34, 241, 282, 434, 565, 595, 616, 621
AflIII ACRYGT 1 cut(s) 361
AgsI TTSAA 2 cut(s) 21, 101
AhdI GACNNNNNGTC 1 cut(s) 65
AjnI CCWGG 4 cut(s) 492, 609, 614, 782
AluBI AGCT 4 cut(s) 105, 174, 524, 676
AluI AGCT 4 cut(s) 105, 174, 524, 676
Alw21I GWGCWC 1 cut(s) 176
Alw26I GTCTC 2 cut(s) 52, 545
AlwI GGATC 3 cut(s) 48, 302, 396
Ama87I CYCGRG 1 cut(s) 548
AoxI GGCC 3 cut(s) 243, 612, 662
ApeKI GCWGC 8 cut(s) 131, 134, 140, 348, 489, 521, 676, 758
AspLEI GCGC 2 cut(s) 457, 502
AspS9I GGNCC 2 cut(s) 409, 662
AsuHPI GGTGA 2 cut(s) 187, 610
AsuNHI GCTAGC 1 cut(s) 238
AvaI CYCGRG 1 cut(s) 548
AvaII GGWCC 1 cut(s) 409
AxyI CCTNAGG 2 cut(s) 377, 590
BaeGI GKGCMC 4 cut(s) 361, 441, 546, 571
BanI GGYRCC 2 cut(s) 438, 543
BanII GRGCYC 1 cut(s) 176
BbrPI CACGTG 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 407
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 8 cut(s) 143, 146, 152, 335, 501, 533, 663, 745
BccI CCATC 1 cut(s) 200
BceAI ACGGC 3 cut(s) 230, 553, 649
BciT130I CCWGG 4 cut(s) 494, 611, 616, 784
BclI TGATCA 1 cut(s) 688
BcoDI GTCTC 2 cut(s) 52, 545
BfaI CTAG 2 cut(s) 30, 239
BfmI CTRYAG 2 cut(s) 349, 677
BglI GCCNNNNNGGC 1 cut(s) 143
BlpI GCTNAGC 1 cut(s) 260
Bme1390I CCNGG 4 cut(s) 494, 611, 616, 784
Bme18I GGWCC 1 cut(s) 409
BmeRI GACNNNNNGTC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 548
BmgT120I GGNCC 2 cut(s) 409, 662
BmiI GGNNCC 2 cut(s) 440, 545
BmrFI CCNGG 4 cut(s) 494, 611, 616, 784
BmsI GCATC 2 cut(s) 14, 786
BmtI GCTAGC 1 cut(s) 242
BoxI GACNNNNGTC 1 cut(s) 407
BpiI GAAGAC 1 cut(s) 407
BpmI CTGGAG 1 cut(s) 389
Bpu1102I GCTNAGC 1 cut(s) 260
BpuEI CTTGAG 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 364
BsaHI GRCGYC 1 cut(s) 251
BsaJI CCNNGG 3 cut(s) 405, 615, 783
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 8 cut(s) 34, 241, 282, 434, 565, 595, 616, 621
Bse1I ACTGG 2 cut(s) 254, 296
Bse21I CCTNAGG 2 cut(s) 377, 590
BseBI CCWGG 4 cut(s) 494, 611, 616, 784
BseDI CCNNGG 3 cut(s) 405, 615, 783
BseLI CCNNNNNNNGG 8 cut(s) 34, 241, 282, 434, 565, 595, 616, 621
BseMII CTCAG 2 cut(s) 251, 581
BseNI ACTGG 2 cut(s) 254, 296
BseRI GAGGAG 3 cut(s) 545, 719, 722
BseSI GKGCMC 4 cut(s) 361, 441, 546, 571
BseX3I CGGCCG 1 cut(s) 243
BseXI GCAGC 8 cut(s) 143, 146, 152, 335, 501, 533, 663, 745
BseYI CCCAGC 1 cut(s) 761
BsgI GTGCAG 1 cut(s) 34
Bsh1236I CGCG 2 cut(s) 416, 500
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 3 cut(s) 245, 614, 664
BshNI GGYRCC 2 cut(s) 438, 543
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 176
BsiHKCI CYCGRG 1 cut(s) 548
BslFI GGGAC 6 cut(s) 217, 259, 302, 328, 617, 800
BslI CCNNNNNNNGG 8 cut(s) 34, 241, 282, 434, 565, 595, 616, 621
BsmAI GTCTC 2 cut(s) 52, 545
BsmFI GGGAC 6 cut(s) 217, 259, 302, 328, 617, 800
BsnI GGCC 3 cut(s) 245, 614, 664
BsoBI CYCGRG 1 cut(s) 548
Bsp1286I GDGCHC 5 cut(s) 176, 361, 441, 546, 571
Bsp143I GATC 4 cut(s) 40, 294, 388, 688
Bsp1720I GCTNAGC 1 cut(s) 260
BspANI GGCC 3 cut(s) 245, 614, 664
BspCNI CTCAG 2 cut(s) 252, 582
BspFNI CGCG 2 cut(s) 416, 500
BspLI GGNNCC 2 cut(s) 440, 545
BspMAI CTGCAG 2 cut(s) 353, 681
BspOI GCTAGC 1 cut(s) 242
BspPI GGATC 3 cut(s) 48, 302, 396
BspT107I GGYRCC 2 cut(s) 438, 543
BsrI ACTGG 2 cut(s) 254, 296
BssECI CCNNGG 3 cut(s) 405, 615, 783
BssMI GATC 4 cut(s) 40, 294, 388, 688
BssNI GRCGYC 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 405
Bst2UI CCWGG 4 cut(s) 494, 611, 616, 784
Bst4CI ACNGT 3 cut(s) 55, 219, 231
Bst6I CTCTTC 1 cut(s) 641
BstACI GRCGYC 1 cut(s) 251
BstBAI YACGTR 1 cut(s) 364
BstC8I GCNNGC 5 cut(s) 240, 329, 424, 480, 542
BstDEI CTNAG 3 cut(s) 260, 377, 590
BstENI CCTNNNNNAGG 1 cut(s) 593
BstFNI CGCG 2 cut(s) 416, 500
BstHHI GCGC 2 cut(s) 457, 502
BstKTI GATC 4 cut(s) 43, 297, 391, 691
BstMAI GTCTC 2 cut(s) 52, 545
BstMBI GATC 4 cut(s) 40, 294, 388, 688
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 9 cut(s) 137, 140, 143, 146, 149, 155, 508, 764, 773
BstNI CCWGG 4 cut(s) 494, 611, 616, 784
BstPAI GACNNNNGTC 1 cut(s) 407
BstSCI CCNGG 4 cut(s) 492, 609, 614, 782
BstSFI CTRYAG 2 cut(s) 349, 677
BstSLI GKGCMC 4 cut(s) 361, 441, 546, 571
BstUI CGCG 2 cut(s) 416, 500
BstV1I GCAGC 8 cut(s) 143, 146, 152, 335, 501, 533, 663, 745
BstV2I GAAGAC 1 cut(s) 407
BstZI CGGCCG 1 cut(s) 243
Bsu36I CCTNAGG 2 cut(s) 377, 590
BsuRI GGCC 3 cut(s) 245, 614, 664
BtsI GCAGTG 3 cut(s) 165, 358, 582
BtsIMutI CAGTG 5 cut(s) 60, 165, 261, 358, 582
Cac8I GCNNGC 5 cut(s) 240, 329, 424, 480, 542
CfoI GCGC 2 cut(s) 457, 502
Cfr13I GGNCC 2 cut(s) 409, 662
CseI GACGC 3 cut(s) 259, 428, 506
Csp6I GTAC 3 cut(s) 120, 310, 586
CviAII CATG 6 cut(s) 124, 465, 698, 716, 725, 793
CviQI GTAC 3 cut(s) 120, 310, 586
DdeI CTNAG 3 cut(s) 260, 377, 590
DpnI GATC 4 cut(s) 42, 296, 390, 690
DpnII GATC 4 cut(s) 40, 294, 388, 688
DriI GACNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 1 cut(s) 243
EagI CGGCCG 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 641
Eam1105I GACNNNNNGTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 641
Ecl136II GAGCTC 1 cut(s) 174
EclXI CGGCCG 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 176
Eco47I GGWCC 1 cut(s) 409
Eco52I CGGCCG 1 cut(s) 243
Eco53kI GAGCTC 1 cut(s) 174
Eco72I CACGTG 1 cut(s) 364
Eco81I CCTNAGG 2 cut(s) 377, 590
Eco88I CYCGRG 1 cut(s) 548
EcoICRI GAGCTC 1 cut(s) 174
EcoNI CCTNNNNNAGG 1 cut(s) 593
EcoRII CCWGG 4 cut(s) 492, 609, 614, 782
EcoT14I CCWWGG 1 cut(s) 405
EcoT38I GRGCYC 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 6 cut(s) 127, 468, 701, 719, 728, 796
FaqI GGGAC 6 cut(s) 217, 259, 302, 328, 617, 800
FatI CATG 6 cut(s) 123, 464, 697, 715, 724, 792
FauI CCCGC 5 cut(s) 243, 279, 336, 353, 533
FbaI TGATCA 1 cut(s) 688
FriOI GRGCYC 1 cut(s) 176
FspBI CTAG 2 cut(s) 30, 239
GlaI GCGC 2 cut(s) 456, 501
GsaI CCCAGC 1 cut(s) 765
GsuI CTGGAG 1 cut(s) 389
HaeIII GGCC 3 cut(s) 245, 614, 664
HgaI GACGC 3 cut(s) 259, 428, 506
HhaI GCGC 2 cut(s) 457, 502
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 6 cut(s) 127, 468, 701, 719, 728, 796
Hin6I GCGC 2 cut(s) 455, 500
HinP1I GCGC 2 cut(s) 455, 500
HinfI GANTC 2 cut(s) 272, 788
HphI GGTGA 2 cut(s) 187, 610
Hpy166II GTNNAC 2 cut(s) 120, 412
Hpy188I TCNGA 3 cut(s) 45, 742, 818
Hpy188III TCNNGA 3 cut(s) 21, 316, 397
Hpy8I GTNNAC 2 cut(s) 120, 412
HpyCH4III ACNGT 3 cut(s) 55, 219, 231
HpyCH4IV ACGT 3 cut(s) 16, 299, 363
HpyCH4V TGCA 3 cut(s) 51, 351, 679
HpyF10VI GCNNNNNNNGC 9 cut(s) 137, 140, 143, 146, 149, 155, 508, 764, 773
HpyF3I CTNAG 3 cut(s) 260, 377, 590
HpySE526I ACGT 3 cut(s) 16, 299, 363
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 6 cut(s) 127, 468, 701, 719, 728, 796
HspAI GCGC 2 cut(s) 455, 500
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 4 cut(s) 40, 294, 388, 688
LmnI GCTCC 1 cut(s) 179
Lsp1109I GCAGC 8 cut(s) 143, 146, 152, 335, 501, 533, 663, 745
LweI GCATC 2 cut(s) 14, 786
MaeI CTAG 2 cut(s) 30, 239
MaeII ACGT 3 cut(s) 16, 299, 363
MaeIII GTNAC 3 cut(s) 12, 247, 666
MalI GATC 4 cut(s) 42, 296, 390, 690
MboI GATC 4 cut(s) 40, 294, 388, 688
MboII GAAGA 4 cut(s) 412, 652, 655, 658
MhlI GDGCHC 5 cut(s) 176, 361, 441, 546, 571
MluCI AATT 4 cut(s) 86, 166, 392, 730
MlyI GAGTC 2 cut(s) 281, 782
MseI TTAA 2 cut(s) 90, 336
MspA1I CMGCKG 3 cut(s) 143, 348, 676
MspR9I CCNGG 4 cut(s) 494, 611, 616, 784
MvaI CCWGG 4 cut(s) 494, 611, 616, 784
MvnI CGCG 2 cut(s) 416, 500
MwoI GCNNNNNNNGC 9 cut(s) 137, 140, 143, 146, 149, 155, 508, 764, 773
NdeII GATC 4 cut(s) 40, 294, 388, 688
NheI GCTAGC 1 cut(s) 238
NlaIII CATG 6 cut(s) 127, 468, 701, 719, 728, 796
NlaIV GGNNCC 2 cut(s) 440, 545
NmuCI GTSAC 2 cut(s) 247, 666
PleI GAGTC 2 cut(s) 280, 782
PmaCI CACGTG 1 cut(s) 364
PmlI CACGTG 1 cut(s) 364
PpsI GAGTC 2 cut(s) 280, 782
Ppu21I YACGTR 1 cut(s) 364
PshAI GACNNNNGTC 1 cut(s) 407
Psp124BI GAGCTC 1 cut(s) 176
Psp6I CCWGG 4 cut(s) 492, 609, 614, 782
PspCI CACGTG 1 cut(s) 364
PspFI CCCAGC 1 cut(s) 761
PspGI CCWGG 4 cut(s) 492, 609, 614, 782
PspN4I GGNNCC 2 cut(s) 440, 545
PspPI GGNCC 2 cut(s) 409, 662
PsrI GAACNNNNNNTAC 2 cut(s) 721, 753
PstI CTGCAG 2 cut(s) 353, 681
PvuII CAGCTG 1 cut(s) 676
RsaI GTAC 3 cut(s) 121, 311, 587
RsaNI GTAC 3 cut(s) 120, 310, 586
SacI GAGCTC 1 cut(s) 176
SaqAI TTAA 2 cut(s) 90, 336
Sau3AI GATC 4 cut(s) 40, 294, 388, 688
Sau96I GGNCC 2 cut(s) 409, 662
SchI GAGTC 2 cut(s) 281, 782
ScrFI CCNGG 4 cut(s) 494, 611, 616, 784
SduI GDGCHC 5 cut(s) 176, 361, 441, 546, 571
SfaNI GCATC 2 cut(s) 14, 786
SfcI CTRYAG 2 cut(s) 349, 677
SinI GGWCC 1 cut(s) 409
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 4 cut(s) 86, 166, 392, 730
SspMI CTAG 2 cut(s) 30, 239
SstI GAGCTC 1 cut(s) 176
StyD4I CCNGG 4 cut(s) 492, 609, 614, 782
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 3 cut(s) 55, 219, 231
TaiI ACGT 3 cut(s) 19, 302, 366
TaqI TCGA 1 cut(s) 170
TasI AATT 4 cut(s) 86, 166, 392, 730
TauI GCSGC 8 cut(s) 140, 146, 149, 152, 245, 286, 478, 514
Tru1I TTAA 2 cut(s) 90, 336
Tru9I TTAA 2 cut(s) 90, 336
TscAI CASTG 5 cut(s) 60, 165, 261, 358, 582
TseFI GTSAC 2 cut(s) 247, 666
TseI GCWGC 8 cut(s) 131, 134, 140, 348, 489, 521, 676, 758
Tsp45I GTSAC 2 cut(s) 247, 666
TspDTI ATGAA 1 cut(s) 759
TspRI CASTG 5 cut(s) 60, 165, 261, 358, 582
VpaK11BI GGWCC 1 cut(s) 409
XagI CCTNNNNNAGG 1 cut(s) 593
XspI CTAG 2 cut(s) 30, 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.