RLG00000032333

Protein of unknown function (DUF640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014900
Physical Location & Seq
Forward (+)
13627356 .. 13628474
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032333

Sequence Viewer

Length: 792 bp
ATGAAAGGTCCTGGTTTCGTACATGAGAGATTTGACGTTTTTGGAGCAGCGGCAGCCGCGGCAGCTGCAGCAGCTGCAGCAGTGGTGGTGAGCCGTTCGAGCTCCAGCAACAGCAGCTCGAGCGCGGAGACTTACCACCACCTCCACCATCACCAATCGTTCATGGCACCATCGCTGCCAGTGGCGCCTCAGCTCAGCCGTTACGAGTCGCAGAAGAGGAGGGACTGGATCACTTTTGGTCAGTATCTGAAGAACCATCGCCCGCCGCTGACTCTGTCACGGTGCAGCGGAGCCCACGTGCTGGAGTTTCTGAGGTACTTGGACCAGTTCGGGAAGACCAAGGTCCACGGGAGTGCCTGCCCTTTCTACGGCCACCCCCACCCGCCAGCGCCGTGTCCCTGCCCGCTGCGGCAGGCCTGGGGTAGCCTGGACGCGCTCATTGGGCGGCTGAGGGCAGCGTTCGAGGAGAACGGCGGGCTGCCAGAGACAAATCCTTTCGGCGCACGCGCCGTGAGGCTGTACCTGAGGGAAGTGAGGGACGCGCAGGCCAAAGCTAGAGGGATTGCTTATGAGAAGAAGAAGCGAAAGAAGCCAACGCCACCACAAAGGACGACACAACTGCTGCAGCAGCATGAGGAGCAAGGGCAACATGGTAATTTTGTTTCTGGTTACGGTGGTGCTGGGGTTGGAGTTGGGGGTGGCTCAGCTCAGCCAAATGGGGTTATGCTCGGTGCAGCTTCGTCTACTGCTGCGAGAGGATCCATGATGCCGCTCTCAGTGTTGAACTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF640

Protein Analysis

264

Amino Acids

28.25

Weight (kDa)

9.9

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALOG_dom PF04852 62 - 180 1.5e-64 ALOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014900)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11120
malus_domestica MD05G1264700.v1.1 MD10G1242600.v1.1
prunus_persica Prupe.4G101300_v2.0.a1
pyrus_communis pycom05g24860 pycom10g20310
rosa_chinensis RchiOBHm_Chr5g0018011
rosa_laevigata RLG00000032333
rosa_multiflora Rmu_sc0010280.1_g000005
rosa_roxburghii Rroxscaffold_1G00059600
rosa_rugosa Rorug05G0039600
rosa_samantha Rh5AG132500 Rh5BG130700 Rh5CG143000 Rh5DG132700
rosa_wichuraiana Rw5G011540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 166, 184
AccB7I CCANNNNNTGG 1 cut(s) 301
AccBSI CCGCTC 1 cut(s) 772
AccI GTMKAC 1 cut(s) 743
AccII CGCG 5 cut(s) 59, 125, 434, 507, 542
AclWI GGATC 3 cut(s) 236, 753, 766
AcoI YGGCCR 1 cut(s) 370
AcuI CTGAAG 1 cut(s) 269
AcvI CACGTG 1 cut(s) 298
AcyI GRCGYC 1 cut(s) 185
AfaI GTAC 3 cut(s) 21, 317, 521
AfiI CCNNNNNNNGG 2 cut(s) 301, 368
AgsI TTSAA 1 cut(s) 784
AjnI CCWGG 3 cut(s) 10, 416, 426
AleI CACNNNNGTG 1 cut(s) 351
AluBI AGCT 8 cut(s) 65, 74, 102, 117, 193, 554, 707, 737
AluI AGCT 8 cut(s) 65, 74, 102, 117, 193, 554, 707, 737
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 2 cut(s) 122, 479
AlwI GGATC 3 cut(s) 236, 753, 766
AlwNI CAGNNNCTG 2 cut(s) 74, 247
Ama87I CYCGRG 1 cut(s) 118
AoxI GGCC 3 cut(s) 370, 414, 546
AspLEI GCGC 7 cut(s) 125, 187, 391, 436, 503, 509, 544
AspS9I GGNCC 3 cut(s) 8, 322, 343
AsuHPI GGTGA 2 cut(s) 100, 143
AvaI CYCGRG 1 cut(s) 118
AvaII GGWCC 3 cut(s) 8, 322, 343
AxyI CCTNAGG 1 cut(s) 524
BamHI GGATCC 1 cut(s) 758
BanI GGYRCC 2 cut(s) 166, 184
BanII GRGCYC 2 cut(s) 104, 295
BbrPI CACGTG 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 104
BbvCI CCTCAGC 2 cut(s) 189, 449
BccI CCATC 3 cut(s) 156, 178, 264
BceAI ACGGC 6 cut(s) 78, 183, 376, 385, 487, 494
BcgI CGANNNNNNTGC 2 cut(s) 601, 635
BciT130I CCWGG 3 cut(s) 12, 418, 428
BcoDI GTCTC 2 cut(s) 122, 479
BfaI CTAG 1 cut(s) 555
BfmI CTRYAG 3 cut(s) 66, 75, 623
BfoI RGCGCY 2 cut(s) 188, 392
BlpI GCTNAGC 3 cut(s) 194, 703, 708
Bme1390I CCNGG 3 cut(s) 12, 418, 428
Bme18I GGWCC 3 cut(s) 8, 322, 343
BmeT110I CYCGRG 1 cut(s) 118
BmgT120I GGNCC 3 cut(s) 8, 322, 343
BmiI GGNNCC 4 cut(s) 168, 186, 292, 760
BmrFI CCNGG 3 cut(s) 12, 418, 428
BmsI GCATC 1 cut(s) 756
BoxI GACNNNNGTC 1 cut(s) 341
BpiI GAAGAC 1 cut(s) 341
BpmI CTGGAG 2 cut(s) 88, 323
Bpu10I CCTNAGC 2 cut(s) 189, 449
Bpu1102I GCTNAGC 3 cut(s) 194, 703, 708
BsaAI YACGTR 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 185
BsaJI CCNNGG 4 cut(s) 57, 339, 346, 417
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 368
Bse1I ACTGG 3 cut(s) 179, 230, 325
Bse21I CCTNAGG 1 cut(s) 524
BseBI CCWGG 3 cut(s) 12, 418, 428
BseDI CCNNGG 4 cut(s) 57, 339, 346, 417
BseLI CCNNNNNNNGG 2 cut(s) 301, 368
BseMII CTCAG 8 cut(s) 203, 208, 302, 440, 515, 717, 722, 789
BseNI ACTGG 3 cut(s) 179, 230, 325
BseRI GAGGAG 3 cut(s) 232, 479, 650
BseYI CCCAGC 1 cut(s) 680
BsgI GTGCAG 2 cut(s) 304, 753
Bsh1236I CGCG 5 cut(s) 59, 125, 434, 507, 542
BshFI GGCC 3 cut(s) 372, 416, 548
BshNI GGYRCC 2 cut(s) 166, 184
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 1 cut(s) 118
BslFI GGGAC 3 cut(s) 236, 381, 551
BslI CCNNNNNNNGG 2 cut(s) 301, 368
BsmAI GTCTC 2 cut(s) 122, 479
BsmFI GGGAC 3 cut(s) 236, 381, 551
BsnI GGCC 3 cut(s) 372, 416, 548
BsoBI CYCGRG 1 cut(s) 118
Bsp1286I GDGCHC 2 cut(s) 104, 295
Bsp143I GATC 2 cut(s) 228, 758
Bsp1720I GCTNAGC 3 cut(s) 194, 703, 708
BspANI GGCC 3 cut(s) 372, 416, 548
BspCNI CTCAG 8 cut(s) 202, 207, 303, 441, 516, 716, 721, 788
BspFNI CGCG 5 cut(s) 59, 125, 434, 507, 542
BspLI GGNNCC 4 cut(s) 168, 186, 292, 760
BspMAI CTGCAG 3 cut(s) 70, 79, 627
BspPI GGATC 3 cut(s) 236, 753, 766
BspT107I GGYRCC 2 cut(s) 166, 184
BsrBI CCGCTC 1 cut(s) 772
BsrI ACTGG 3 cut(s) 179, 230, 325
BssECI CCNNGG 4 cut(s) 57, 339, 346, 417
BssMI GATC 2 cut(s) 228, 758
BssNI GRCGYC 1 cut(s) 185
BssT1I CCWWGG 1 cut(s) 339
Bst2UI CCWGG 3 cut(s) 12, 418, 428
Bst4CI ACNGT 2 cut(s) 282, 674
Bst6I CTCTTC 1 cut(s) 209
BstACI GRCGYC 1 cut(s) 185
BstAPI GCANNNNNTGC 1 cut(s) 74
BstBAI YACGTR 1 cut(s) 298
BstC8I GCNNGC 8 cut(s) 263, 358, 387, 404, 414, 476, 505, 546
BstDEI CTNAG 8 cut(s) 189, 194, 311, 449, 524, 703, 708, 775
BstDSI CCRYGG 2 cut(s) 57, 346
BstFNI CGCG 5 cut(s) 59, 125, 434, 507, 542
BstH2I RGCGCY 2 cut(s) 188, 392
BstHHI GCGC 7 cut(s) 125, 187, 391, 436, 503, 509, 544
BstKTI GATC 2 cut(s) 231, 761
BstMAI GTCTC 2 cut(s) 122, 479
BstMBI GATC 2 cut(s) 228, 758
BstNI CCWGG 3 cut(s) 12, 418, 428
BstPAI GACNNNNGTC 1 cut(s) 341
BstSCI CCNGG 3 cut(s) 10, 416, 426
BstSFI CTRYAG 3 cut(s) 66, 75, 623
BstUI CGCG 5 cut(s) 59, 125, 434, 507, 542
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 758
BstYI RGATCY 1 cut(s) 758
Bsu36I CCTNAGG 1 cut(s) 524
BsuRI GGCC 3 cut(s) 372, 416, 548
BtgI CCRYGG 2 cut(s) 57, 346
BtgZI GCGATG 2 cut(s) 156, 242
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 3 cut(s) 87, 186, 783
Cac8I GCNNGC 8 cut(s) 263, 358, 387, 404, 414, 476, 505, 546
CaiI CAGNNNCTG 2 cut(s) 74, 247
CfoI GCGC 7 cut(s) 125, 187, 391, 436, 503, 509, 544
Cfr13I GGNCC 3 cut(s) 8, 322, 343
Cfr42I CCGCGG 1 cut(s) 60
CseI GACGC 2 cut(s) 440, 548
Csp6I GTAC 3 cut(s) 20, 316, 520
CviAII CATG 5 cut(s) 23, 163, 632, 650, 763
CviQI GTAC 3 cut(s) 20, 316, 520
DdeI CTNAG 8 cut(s) 189, 194, 311, 449, 524, 703, 708, 775
DinI GGCGCC 1 cut(s) 186
DpnI GATC 2 cut(s) 230, 760
DpnII GATC 2 cut(s) 228, 758
EaeI YGGCCR 1 cut(s) 370
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
Ecl136II GAGCTC 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 339
Eco147I AGGCCT 1 cut(s) 416
Eco24I GRGCYC 2 cut(s) 104, 295
Eco47I GGWCC 3 cut(s) 8, 322, 343
Eco53kI GAGCTC 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 269
Eco72I CACGTG 1 cut(s) 298
Eco81I CCTNAGG 1 cut(s) 524
Eco88I CYCGRG 1 cut(s) 118
EcoICRI GAGCTC 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 8
EcoRII CCWGG 3 cut(s) 10, 416, 426
EcoT14I CCWWGG 1 cut(s) 339
EcoT38I GRGCYC 2 cut(s) 104, 295
EgeI GGCGCC 1 cut(s) 186
EheI GGCGCC 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 339
FaeI CATG 5 cut(s) 26, 166, 635, 653, 766
FaiI YATR 7 cut(s) 24, 164, 570, 633, 651, 725, 764
FaqI GGGAC 3 cut(s) 236, 381, 551
FatI CATG 5 cut(s) 22, 162, 631, 649, 762
FauI CCCGC 4 cut(s) 270, 390, 411, 467
FblI GTMKAC 1 cut(s) 743
FriOI GRGCYC 2 cut(s) 104, 295
FspBI CTAG 1 cut(s) 555
GlaI GCGC 7 cut(s) 124, 186, 390, 435, 502, 508, 543
GsaI CCCAGC 1 cut(s) 684
GsuI CTGGAG 2 cut(s) 88, 323
HaeII RGCGCY 2 cut(s) 188, 392
HaeIII GGCC 3 cut(s) 372, 416, 548
HgaI GACGC 2 cut(s) 440, 548
HhaI GCGC 7 cut(s) 125, 187, 391, 436, 503, 509, 544
Hin1I GRCGYC 1 cut(s) 185
Hin1II CATG 5 cut(s) 26, 166, 635, 653, 766
Hin6I GCGC 7 cut(s) 123, 185, 389, 434, 501, 507, 542
HinP1I GCGC 7 cut(s) 123, 185, 389, 434, 501, 507, 542
HinfI GANTC 2 cut(s) 206, 271
HphI GGTGA 2 cut(s) 100, 143
Hpy166II GTNNAC 2 cut(s) 346, 744
Hpy188I TCNGA 2 cut(s) 249, 312
Hpy188III TCNNGA 1 cut(s) 331
Hpy8I GTNNAC 2 cut(s) 346, 744
HpyCH4III ACNGT 2 cut(s) 282, 674
HpyCH4IV ACGT 2 cut(s) 36, 297
HpyCH4V TGCA 5 cut(s) 68, 77, 285, 625, 734
HpyF3I CTNAG 8 cut(s) 189, 194, 311, 449, 524, 703, 708, 775
HpySE526I ACGT 2 cut(s) 36, 297
Hsp92I GRCGYC 1 cut(s) 185
Hsp92II CATG 5 cut(s) 26, 166, 635, 653, 766
HspAI GCGC 7 cut(s) 123, 185, 389, 434, 501, 507, 542
KasI GGCGCC 1 cut(s) 184
KspI CCGCGG 1 cut(s) 60
Kzo9I GATC 2 cut(s) 228, 758
LmnI GCTCC 4 cut(s) 44, 107, 290, 637
LweI GCATC 1 cut(s) 756
MaeI CTAG 1 cut(s) 555
MaeII ACGT 2 cut(s) 36, 297
MaeIII GTNAC 3 cut(s) 200, 276, 668
MalI GATC 2 cut(s) 230, 760
MbiI CCGCTC 1 cut(s) 772
MboI GATC 2 cut(s) 228, 758
MboII GAAGA 5 cut(s) 226, 262, 346, 586, 589
MflI RGATCY 1 cut(s) 758
MhlI GDGCHC 2 cut(s) 104, 295
MluCI AATT 1 cut(s) 655
Mly113I GGCGCC 1 cut(s) 185
MlyI GAGTC 2 cut(s) 215, 265
MmeI TCCRAC 1 cut(s) 667
MslI CAYNNNNRTG 1 cut(s) 351
MspA1I CMGCKG 7 cut(s) 50, 59, 65, 74, 268, 288, 406
MspR9I CCNGG 3 cut(s) 12, 418, 428
MvaI CCWGG 3 cut(s) 12, 418, 428
MvnI CGCG 5 cut(s) 59, 125, 434, 507, 542
NarI GGCGCC 1 cut(s) 185
NdeII GATC 2 cut(s) 228, 758
NlaIII CATG 5 cut(s) 26, 166, 635, 653, 766
NlaIV GGNNCC 4 cut(s) 168, 186, 292, 760
NmuCI GTSAC 1 cut(s) 276
OliI CACNNNNGTG 1 cut(s) 351
PaeR7I CTCGAG 1 cut(s) 118
PceI AGGCCT 1 cut(s) 416
PflFI GACNNNGTC 1 cut(s) 274
PflMI CCANNNNNTGG 1 cut(s) 301
PleI GAGTC 2 cut(s) 214, 265
PluTI GGCGCC 1 cut(s) 188
PmaCI CACGTG 1 cut(s) 298
PmlI CACGTG 1 cut(s) 298
PpsI GAGTC 2 cut(s) 214, 265
Ppu21I YACGTR 1 cut(s) 298
PpuMI RGGWCCY 1 cut(s) 8
PshAI GACNNNNGTC 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 104
Psp5II RGGWCCY 1 cut(s) 8
Psp6I CCWGG 3 cut(s) 10, 416, 426
PspCI CACGTG 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 680
PspGI CCWGG 3 cut(s) 10, 416, 426
PspN4I GGNNCC 4 cut(s) 168, 186, 292, 760
PspPI GGNCC 3 cut(s) 8, 322, 343
PspPPI RGGWCCY 1 cut(s) 8
PspXI VCTCGAGB 1 cut(s) 118
PstI CTGCAG 3 cut(s) 70, 79, 627
PstNI CAGNNNCTG 2 cut(s) 74, 247
PsuI RGATCY 1 cut(s) 758
PsyI GACNNNGTC 1 cut(s) 274
PvuII CAGCTG 2 cut(s) 65, 74
RsaI GTAC 3 cut(s) 21, 317, 521
RsaNI GTAC 3 cut(s) 20, 316, 520
RseI CAYNNNNRTG 1 cut(s) 351
SacI GAGCTC 1 cut(s) 104
SacII CCGCGG 1 cut(s) 60
Sau3AI GATC 2 cut(s) 228, 758
Sau96I GGNCC 3 cut(s) 8, 322, 343
SchI GAGTC 2 cut(s) 215, 265
ScrFI CCNGG 3 cut(s) 12, 418, 428
SduI GDGCHC 2 cut(s) 104, 295
SfaNI GCATC 1 cut(s) 756
SfcI CTRYAG 3 cut(s) 66, 75, 623
SfoI GGCGCC 1 cut(s) 186
Sfr274I CTCGAG 1 cut(s) 118
Sfr303I CCGCGG 1 cut(s) 60
SgrBI CCGCGG 1 cut(s) 60
SinI GGWCC 3 cut(s) 8, 322, 343
SlaI CTCGAG 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 351
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
Sse9I AATT 1 cut(s) 655
SseBI AGGCCT 1 cut(s) 416
SspDI GGCGCC 1 cut(s) 184
SspMI CTAG 1 cut(s) 555
SstI GAGCTC 1 cut(s) 104
StuI AGGCCT 1 cut(s) 416
StyD4I CCNGG 3 cut(s) 10, 416, 426
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 2 cut(s) 282, 674
TaiI ACGT 2 cut(s) 39, 300
TaqI TCGA 3 cut(s) 98, 119, 462
TasI AATT 1 cut(s) 655
TauI GCSGC 7 cut(s) 53, 59, 62, 268, 412, 448, 772
TscAI CASTG 3 cut(s) 87, 186, 783
TseFI GTSAC 1 cut(s) 276
Tsp45I GTSAC 1 cut(s) 276
TspDTI ATGAA 2 cut(s) 17, 151
TspRI CASTG 3 cut(s) 87, 186, 783
Tth111I GACNNNGTC 1 cut(s) 274
Van91I CCANNNNNTGG 1 cut(s) 301
VpaK11BI GGWCC 3 cut(s) 8, 322, 343
XhoI CTCGAG 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 743
XspI CTAG 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.