Rh5DG132700

Protein of unknown function (DUF640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0014900
Physical Location & Seq
Forward (+)
13128060 .. 13128809
750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG132700.1

Sequence Viewer

Length: 750 bp
ATGTCAGCAGCGGCGGCCGCGGCAGCTGCAGCTGCAGCAGTGGCTGTGAGCCGTTCGAGCTCCAGCAACAGCAGCTCGAGCGCGGAGACCTACCACCACCTCCACCATCACCAATCGTTCATGGCACCGTCGCTACCTGTGGCGCCTCAGCTCAGCCGTTACGAGTCGCAGAAGAGGAGGGACTGGATCACTTTTGGTCAGTATCTGAAGAACCATCGCCCGCCGCTGACTCTGTCACGGTGCAGCGGAGCCCACGTGCTGGAGTTTCTGAGGTACTTGGACCAGTTCGGGAAGACCAAGGTCCACGGGAGTGCCTGCCCTTTCTATGGCCACCCCCACCCGCCAGCGCCGTGTCCCTGCCCGCTGCGGCAGGCCTGGGGCAGCCTGGACGCGCTCATTGGGCGGCTGAGGGCAGCGTTCGAGGAGAATGGCGGGCTGCCAGAGACAAACCCTTTCGGCGCACGCGCCGTGAGACTGTACCTGAGGGAAGTGAGGGACACGCAGGCCAAAGCTAGAGGGATTGCTTATGAGAAGAAGAAGCGAAAGAAGCCAACGCCACCACATAGGACGACACAACTGCTGCAGCAGCACGAGGAGCAAGGGCAACATGGCAATTTTGTTTCTGGTTTCGGTGGTGCTGGGGTTGGAGTTGGTGGTGGCTCAGCTCAGCCAAATGGGGTTATGCTCGGTGGAGCTTCGTCTACTGCTGCGAGAGGATCCATGATGCCGCTATCAGTGTTGAACTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF640

Protein Analysis

249

Amino Acids

26.67

Weight (kDa)

9.98

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALOG_dom PF04852 48 - 166 1.3e-64 ALOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014900)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11120
malus_domestica MD05G1264700.v1.1 MD10G1242600.v1.1
prunus_persica Prupe.4G101300_v2.0.a1
pyrus_communis pycom05g24860 pycom10g20310
rosa_chinensis RchiOBHm_Chr5g0018011
rosa_laevigata RLG00000032333
rosa_multiflora Rmu_sc0010280.1_g000005
rosa_roxburghii Rroxscaffold_1G00059600
rosa_rugosa Rorug05G0039600
rosa_samantha Rh5AG132500 Rh5BG130700 Rh5CG143000 Rh5DG132700
rosa_wichuraiana Rw5G011540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 124, 142
AccB7I CCANNNNNTGG 1 cut(s) 259
AccI GTMKAC 1 cut(s) 701
AccII CGCG 4 cut(s) 20, 83, 392, 465
AclWI GGATC 3 cut(s) 194, 711, 724
AcoI YGGCCR 2 cut(s) 15, 328
AcuI CTGAAG 1 cut(s) 227
AcvI CACGTG 1 cut(s) 256
AcyI GRCGYC 1 cut(s) 143
AfaI GTAC 2 cut(s) 275, 479
AfiI CCNNNNNNNGG 2 cut(s) 259, 326
AgsI TTSAA 1 cut(s) 742
AjnI CCWGG 2 cut(s) 374, 384
AleI CACNNNNGTG 1 cut(s) 309
AluBI AGCT 8 cut(s) 26, 32, 60, 75, 151, 512, 665, 695
AluI AGCT 8 cut(s) 26, 32, 60, 75, 151, 512, 665, 695
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 3 cut(s) 80, 437, 466
AlwI GGATC 3 cut(s) 194, 711, 724
AlwNI CAGNNNCTG 2 cut(s) 44, 205
Ama87I CYCGRG 1 cut(s) 76
AoxI GGCC 4 cut(s) 15, 328, 372, 504
AspLEI GCGC 6 cut(s) 83, 145, 349, 394, 461, 467
AspS9I GGNCC 2 cut(s) 280, 301
AsuHPI GGTGA 1 cut(s) 101
AvaI CYCGRG 1 cut(s) 76
AvaII GGWCC 2 cut(s) 280, 301
AxyI CCTNAGG 1 cut(s) 482
BalI TGGCCA 1 cut(s) 330
BamHI GGATCC 1 cut(s) 716
BanI GGYRCC 2 cut(s) 124, 142
BanII GRGCYC 2 cut(s) 62, 253
BauI CACGAG 1 cut(s) 590
BbrPI CACGTG 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 299
Bbv12I GWGCWC 1 cut(s) 62
BbvCI CCTCAGC 2 cut(s) 147, 407
BccI CCATC 2 cut(s) 114, 222
BceAI ACGGC 4 cut(s) 36, 141, 334, 452
BcgI CGANNNNNNTGC 2 cut(s) 559, 593
BciT130I CCWGG 2 cut(s) 376, 386
BcoDI GTCTC 3 cut(s) 80, 437, 466
BfaI CTAG 1 cut(s) 513
BfmI CTRYAG 3 cut(s) 27, 33, 581
BfoI RGCGCY 2 cut(s) 146, 350
BlpI GCTNAGC 3 cut(s) 152, 661, 666
Bme1390I CCNGG 2 cut(s) 376, 386
Bme18I GGWCC 2 cut(s) 280, 301
BmeT110I CYCGRG 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 280, 301
BmiI GGNNCC 4 cut(s) 126, 144, 250, 718
BmrFI CCNGG 2 cut(s) 376, 386
BmsI GCATC 1 cut(s) 714
BoxI GACNNNNGTC 1 cut(s) 299
BpiI GAAGAC 1 cut(s) 299
BpmI CTGGAG 2 cut(s) 46, 281
Bpu10I CCTNAGC 2 cut(s) 147, 407
Bpu1102I GCTNAGC 3 cut(s) 152, 661, 666
BsaAI YACGTR 1 cut(s) 256
BsaHI GRCGYC 1 cut(s) 143
BsaI GGTCTC 1 cut(s) 80
BsaJI CCNNGG 4 cut(s) 18, 297, 304, 375
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 326
Bse1I ACTGG 2 cut(s) 188, 283
Bse21I CCTNAGG 1 cut(s) 482
BseBI CCWGG 2 cut(s) 376, 386
BseDI CCNNGG 4 cut(s) 18, 297, 304, 375
BseLI CCNNNNNNNGG 2 cut(s) 259, 326
BseMII CTCAG 7 cut(s) 161, 166, 260, 398, 473, 675, 680
BseNI ACTGG 2 cut(s) 188, 283
BseRI GAGGAG 3 cut(s) 190, 437, 608
BseX3I CGGCCG 1 cut(s) 15
BseYI CCCAGC 1 cut(s) 638
BsgI GTGCAG 1 cut(s) 262
Bsh1236I CGCG 4 cut(s) 20, 83, 392, 465
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 4 cut(s) 17, 330, 374, 506
BshNI GGYRCC 2 cut(s) 124, 142
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 62
BsiHKCI CYCGRG 1 cut(s) 76
BslFI GGGAC 3 cut(s) 194, 339, 509
BslI CCNNNNNNNGG 2 cut(s) 259, 326
BsmAI GTCTC 3 cut(s) 80, 437, 466
BsmFI GGGAC 3 cut(s) 194, 339, 509
BsnI GGCC 4 cut(s) 17, 330, 374, 506
Bso31I GGTCTC 1 cut(s) 80
BsoBI CYCGRG 1 cut(s) 76
Bsp1286I GDGCHC 2 cut(s) 62, 253
Bsp143I GATC 2 cut(s) 186, 716
Bsp1720I GCTNAGC 3 cut(s) 152, 661, 666
BspANI GGCC 4 cut(s) 17, 330, 374, 506
BspCNI CTCAG 7 cut(s) 160, 165, 261, 399, 474, 674, 679
BspFNI CGCG 4 cut(s) 20, 83, 392, 465
BspLI GGNNCC 4 cut(s) 126, 144, 250, 718
BspMAI CTGCAG 3 cut(s) 31, 37, 585
BspPI GGATC 3 cut(s) 194, 711, 724
BspT107I GGYRCC 2 cut(s) 124, 142
BspTNI GGTCTC 1 cut(s) 80
BsrI ACTGG 2 cut(s) 188, 283
BssECI CCNNGG 4 cut(s) 18, 297, 304, 375
BssMI GATC 2 cut(s) 186, 716
BssNI GRCGYC 1 cut(s) 143
BssSI CACGAG 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 590
Bst2UI CCWGG 2 cut(s) 376, 386
Bst4CI ACNGT 3 cut(s) 129, 240, 477
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 1 cut(s) 143
BstBAI YACGTR 1 cut(s) 256
BstC8I GCNNGC 8 cut(s) 221, 316, 345, 362, 372, 434, 463, 504
BstDEI CTNAG 7 cut(s) 147, 152, 269, 407, 482, 661, 666
BstDSI CCRYGG 2 cut(s) 18, 304
BstFNI CGCG 4 cut(s) 20, 83, 392, 465
BstH2I RGCGCY 2 cut(s) 146, 350
BstHHI GCGC 6 cut(s) 83, 145, 349, 394, 461, 467
BstKTI GATC 2 cut(s) 189, 719
BstMAI GTCTC 3 cut(s) 80, 437, 466
BstMBI GATC 2 cut(s) 186, 716
BstMCI CGRYCG 1 cut(s) 18
BstNI CCWGG 2 cut(s) 376, 386
BstPAI GACNNNNGTC 1 cut(s) 299
BstSCI CCNGG 2 cut(s) 374, 384
BstSFI CTRYAG 3 cut(s) 27, 33, 581
BstUI CGCG 4 cut(s) 20, 83, 392, 465
BstV2I GAAGAC 1 cut(s) 299
BstX2I RGATCY 1 cut(s) 716
BstYI RGATCY 1 cut(s) 716
BstZI CGGCCG 1 cut(s) 15
Bsu36I CCTNAGG 1 cut(s) 482
BsuRI GGCC 4 cut(s) 17, 330, 374, 506
BtgI CCRYGG 2 cut(s) 18, 304
BtgZI GCGATG 1 cut(s) 200
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 45, 741
Cac8I GCNNGC 8 cut(s) 221, 316, 345, 362, 372, 434, 463, 504
CaiI CAGNNNCTG 2 cut(s) 44, 205
CciNI GCGGCCGC 1 cut(s) 15
CfoI GCGC 6 cut(s) 83, 145, 349, 394, 461, 467
Cfr13I GGNCC 2 cut(s) 280, 301
Cfr42I CCGCGG 1 cut(s) 21
CseI GACGC 1 cut(s) 398
Csp6I GTAC 2 cut(s) 274, 478
CviAII CATG 3 cut(s) 121, 608, 721
CviQI GTAC 2 cut(s) 274, 478
DdeI CTNAG 7 cut(s) 147, 152, 269, 407, 482, 661, 666
DinI GGCGCC 1 cut(s) 144
DpnI GATC 2 cut(s) 188, 718
DpnII GATC 2 cut(s) 186, 716
EaeI YGGCCR 2 cut(s) 15, 328
EagI CGGCCG 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 60
EclXI CGGCCG 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 297
Eco147I AGGCCT 1 cut(s) 374
Eco24I GRGCYC 2 cut(s) 62, 253
Eco31I GGTCTC 1 cut(s) 80
Eco47I GGWCC 2 cut(s) 280, 301
Eco52I CGGCCG 1 cut(s) 15
Eco53kI GAGCTC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 227
Eco72I CACGTG 1 cut(s) 256
Eco81I CCTNAGG 1 cut(s) 482
Eco88I CYCGRG 1 cut(s) 76
EcoICRI GAGCTC 1 cut(s) 60
EcoRII CCWGG 2 cut(s) 374, 384
EcoT14I CCWWGG 1 cut(s) 297
EcoT38I GRGCYC 2 cut(s) 62, 253
EgeI GGCGCC 1 cut(s) 144
EheI GGCGCC 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 3 cut(s) 124, 611, 724
FaiI YATR 7 cut(s) 122, 327, 528, 564, 609, 683, 722
FaqI GGGAC 3 cut(s) 194, 339, 509
FatI CATG 3 cut(s) 120, 607, 720
FauI CCCGC 4 cut(s) 228, 348, 369, 425
FblI GTMKAC 1 cut(s) 701
FriOI GRGCYC 2 cut(s) 62, 253
FspBI CTAG 1 cut(s) 513
GlaI GCGC 6 cut(s) 82, 144, 348, 393, 460, 466
GsaI CCCAGC 1 cut(s) 642
GsuI CTGGAG 2 cut(s) 46, 281
HaeII RGCGCY 2 cut(s) 146, 350
HaeIII GGCC 4 cut(s) 17, 330, 374, 506
HgaI GACGC 1 cut(s) 398
HhaI GCGC 6 cut(s) 83, 145, 349, 394, 461, 467
Hin1I GRCGYC 1 cut(s) 143
Hin1II CATG 3 cut(s) 124, 611, 724
Hin6I GCGC 6 cut(s) 81, 143, 347, 392, 459, 465
HinP1I GCGC 6 cut(s) 81, 143, 347, 392, 459, 465
HinfI GANTC 2 cut(s) 164, 229
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 2 cut(s) 304, 702
Hpy188I TCNGA 2 cut(s) 207, 270
Hpy188III TCNNGA 1 cut(s) 289
Hpy8I GTNNAC 2 cut(s) 304, 702
Hpy99I CGWCG 1 cut(s) 133
HpyCH4III ACNGT 3 cut(s) 129, 240, 477
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 4 cut(s) 29, 35, 243, 583
HpyF3I CTNAG 7 cut(s) 147, 152, 269, 407, 482, 661, 666
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 1 cut(s) 143
Hsp92II CATG 3 cut(s) 124, 611, 724
HspAI GCGC 6 cut(s) 81, 143, 347, 392, 459, 465
KasI GGCGCC 1 cut(s) 142
KspI CCGCGG 1 cut(s) 21
Kzo9I GATC 2 cut(s) 186, 716
LmnI GCTCC 4 cut(s) 65, 248, 595, 692
LweI GCATC 1 cut(s) 714
MaeI CTAG 1 cut(s) 513
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 2 cut(s) 158, 234
MalI GATC 2 cut(s) 188, 718
MboI GATC 2 cut(s) 186, 716
MboII GAAGA 5 cut(s) 184, 220, 304, 544, 547
MflI RGATCY 1 cut(s) 716
MhlI GDGCHC 2 cut(s) 62, 253
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 1 cut(s) 613
MluNI TGGCCA 1 cut(s) 330
Mly113I GGCGCC 1 cut(s) 143
MlyI GAGTC 2 cut(s) 173, 223
MmeI TCCRAC 1 cut(s) 625
Mox20I TGGCCA 1 cut(s) 330
MscI TGGCCA 1 cut(s) 330
MslI CAYNNNNRTG 1 cut(s) 309
Msp20I TGGCCA 1 cut(s) 330
MspA1I CMGCKG 7 cut(s) 11, 20, 26, 32, 226, 246, 364
MspR9I CCNGG 2 cut(s) 376, 386
MvaI CCWGG 2 cut(s) 376, 386
MvnI CGCG 4 cut(s) 20, 83, 392, 465
NarI GGCGCC 1 cut(s) 143
NdeII GATC 2 cut(s) 186, 716
NlaIII CATG 3 cut(s) 124, 611, 724
NlaIV GGNNCC 4 cut(s) 126, 144, 250, 718
NmuCI GTSAC 1 cut(s) 234
NotI GCGGCCGC 1 cut(s) 15
OliI CACNNNNGTG 1 cut(s) 309
PaeR7I CTCGAG 1 cut(s) 76
PceI AGGCCT 1 cut(s) 374
PflFI GACNNNGTC 1 cut(s) 232
PflMI CCANNNNNTGG 1 cut(s) 259
PleI GAGTC 2 cut(s) 172, 223
PluTI GGCGCC 1 cut(s) 146
PmaCI CACGTG 1 cut(s) 256
PmlI CACGTG 1 cut(s) 256
PpsI GAGTC 2 cut(s) 172, 223
Ppu21I YACGTR 1 cut(s) 256
PshAI GACNNNNGTC 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 62
Psp6I CCWGG 2 cut(s) 374, 384
PspCI CACGTG 1 cut(s) 256
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 2 cut(s) 374, 384
PspN4I GGNNCC 4 cut(s) 126, 144, 250, 718
PspPI GGNCC 2 cut(s) 280, 301
PspXI VCTCGAGB 1 cut(s) 76
PstI CTGCAG 3 cut(s) 31, 37, 585
PstNI CAGNNNCTG 2 cut(s) 44, 205
PsuI RGATCY 1 cut(s) 716
PsyI GACNNNGTC 1 cut(s) 232
PvuII CAGCTG 2 cut(s) 26, 32
RsaI GTAC 2 cut(s) 275, 479
RsaNI GTAC 2 cut(s) 274, 478
RseI CAYNNNNRTG 1 cut(s) 309
SacI GAGCTC 1 cut(s) 62
SacII CCGCGG 1 cut(s) 21
Sau3AI GATC 2 cut(s) 186, 716
Sau96I GGNCC 2 cut(s) 280, 301
SchI GAGTC 2 cut(s) 173, 223
ScrFI CCNGG 2 cut(s) 376, 386
SduI GDGCHC 2 cut(s) 62, 253
SfaNI GCATC 1 cut(s) 714
SfcI CTRYAG 3 cut(s) 27, 33, 581
SfoI GGCGCC 1 cut(s) 144
Sfr274I CTCGAG 1 cut(s) 76
Sfr303I CCGCGG 1 cut(s) 21
SgrBI CCGCGG 1 cut(s) 21
SinI GGWCC 2 cut(s) 280, 301
SlaI CTCGAG 1 cut(s) 76
SmiMI CAYNNNNRTG 1 cut(s) 309
SmlI CTYRAG 1 cut(s) 76
SmoI CTYRAG 1 cut(s) 76
Sse9I AATT 1 cut(s) 613
SseBI AGGCCT 1 cut(s) 374
SspDI GGCGCC 1 cut(s) 142
SspMI CTAG 1 cut(s) 513
SstI GAGCTC 1 cut(s) 62
StuI AGGCCT 1 cut(s) 374
StyD4I CCNGG 2 cut(s) 374, 384
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 3 cut(s) 129, 240, 477
TaiI ACGT 1 cut(s) 258
TaqI TCGA 3 cut(s) 56, 77, 420
TasI AATT 1 cut(s) 613
TauI GCSGC 8 cut(s) 14, 17, 20, 23, 226, 370, 406, 730
TscAI CASTG 2 cut(s) 45, 741
TseFI GTSAC 1 cut(s) 234
Tsp45I GTSAC 1 cut(s) 234
TspDTI ATGAA 1 cut(s) 109
TspRI CASTG 2 cut(s) 45, 741
Tth111I GACNNNGTC 1 cut(s) 232
Van91I CCANNNNNTGG 1 cut(s) 259
VpaK11BI GGWCC 2 cut(s) 280, 301
XhoI CTCGAG 1 cut(s) 76
XmiI GTMKAC 1 cut(s) 701
XspI CTAG 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.