Rorug05G0039600

Protein of unknown function (DUF640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014900
Physical Location & Seq
Reverse (-)
3438073 .. 3452932
14860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0039600.1

Sequence Viewer

Length: 1593 bp
ATGAATCCTCAATATCGAAGTGGGTTTACTCTAGATGATAATGAGGAGCAAGGAACAAAGTACTTAACTTGGGCTACACTATTTTCCTCAACTTTGGCTTATGAAGAAATCTCTTCAGAAGGTACAACTAAGTTTATGGTTTCAGTTAAAGGCAATAACTGGATTCTTGGTTTGGAGGCACCAAAACATCCATGTGACATTTATGGTGCTTGTGGACCTTTTGGATTTTGCAAAGCTTCTGAATCTCCAATCTGCAAGTGTTTGAAAGGGTTTGTTCCCAAGTCCCACCAGGAATGGAGCAAAGGAAACTGGACAGGAGGGTGTGCGAGGAAAACCAAATTGCTTTGTCAGAGACAAACAAATCAGTCAGTCTCATCGAGAGGAAAAGAAGATGATGGGTTTTTGAAGATGGTAAGGCTAAAAGTACCAGATTTGCATGAATTCTATACTTCTTTCGGTCCAGACACATCTGAAAACTGCAAGATCCGATGCCTAAATAACTGTTCTTGTCTGGCTTATGCATTTGTTAATAGTATAGGTTGTTTGGTCTGGTCCAAAAATCTTATTGATATACTAGAGTTTTCTTCTGGTGGAGTAGATGTTTTTATTTGCATACCACGAGCAGAACTGGGTGAAGGCAGGCCAATAAGATTAATTGTCAGTCTCACAACTATTTGTTTGATCAGTATCTTGGGTGCCATAGTGTTTGGTTTGTACAGGTTGCGAGCTAACCAAAGAGGAAAAACCACAGTAGAAAAAGCAAAGTATGAAATGGCTGATAAGATTCAGACTTCACGAGACACTTTAAGAGAATATATTGGAAAAGATGATCCATCTCAGCTATTGATCTATGATTTTGATAGCATATTAGTTGCTACAAACAATTTCAGTCTTGCAAACAAACTCGGGCAAGGAGGTTTTGGCCCAGTTTATAAGGGTATACTACAAGATGGGAAGGAAATAGCTGTAAAAAGACTATCTAGTAGCTCAGGACAAGGCATTGAAGAGTTCAAGAATGAGATGATGTTGATCTCCAATCTTCAACATAAAAATCTTGTTAGGATGATGGGTTGCTGCATTAAAGAGGATGAGAAGTTACTTGTTTATGAGTTCATGCCAAACAAAAGCTTGGATACGTTTCTATTCGATCCGATGAGGACAACAGAACTTGATTGGGATAGACGCTTTAATATTATTCAGGGTGTTATTAGAGGGCTTCTTTATCTCCATTATGATTCCTATCTGAAGGTAATACATAGAGACCTGAAAGTCAGCAACATTCTCCTGGATGAAAAGATGAACCCAAAAATTTCAGATTTTGGATTGGCACGCATAGTTGAAGGAACATCAGAAACCAGTTTCATACTAATGAGTTTTCCACACAATGGCTATATGTCTCCGGAGTATGCCATGGGTGGGATATTTTCCGAAAAATCTGATGTCTACAGCTTTGGGGCATGGAACTTGTGGTATGAAGGCAAGGGATTGGAGTTGGTGGATGAAGTATTGGGTGATTCATATTCCTCATCAGAATATGGACATACTAATTCCTCTACGAATGAAGCTACCATTACAGTGATTGAAGGACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF640

Protein Analysis

530

Amino Acids

59.54

Weight (kDa)

6.01

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 5 - 95 1.8e-20 S-locus glycoprotein domain
PAN_2 PF08276 129 - 191 2.3e-15 PAN-like domain
PK_Tyr_Ser-Thr PF07714 298 - 486 1.1e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 298 - 486 1.2e-37 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014900)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11120
malus_domestica MD05G1264700.v1.1 MD10G1242600.v1.1
prunus_persica Prupe.4G101300_v2.0.a1
pyrus_communis pycom05g24860 pycom10g20310
rosa_chinensis RchiOBHm_Chr5g0018011
rosa_laevigata RLG00000032333
rosa_multiflora Rmu_sc0010280.1_g000005
rosa_roxburghii Rroxscaffold_1G00059600
rosa_rugosa Rorug05G0039600
rosa_samantha Rh5AG132500 Rh5BG130700 Rh5CG143000 Rh5DG132700
rosa_wichuraiana Rw5G011540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 933
AasI GACNNNNNNGTC 1 cut(s) 1268
AccB1I GGYRCC 2 cut(s) 178, 695
AccB7I CCANNNNNTGG 1 cut(s) 1385
AccI GTMKAC 2 cut(s) 940, 1443
AccIII TCCGGA 1 cut(s) 1399
AclWI GGATC 3 cut(s) 478, 824, 1142
AcsI RAATTY 2 cut(s) 440, 1308
AcuI CTGAAG 2 cut(s) 99, 1265
AfaI GTAC 4 cut(s) 62, 124, 426, 716
AfiI CCNNNNNNNGG 2 cut(s) 1385, 1416
AgsI TTSAA 7 cut(s) 265, 406, 1004, 1012, 1043, 1340, 1583
AjnI CCWGG 2 cut(s) 288, 1284
AluBI AGCT 8 cut(s) 236, 728, 841, 965, 987, 1128, 1449, 1565
AluI AGCT 8 cut(s) 236, 728, 841, 965, 987, 1128, 1449, 1565
Alw26I GTCTC 6 cut(s) 346, 376, 668, 792, 1254, 1401
AlwI GGATC 3 cut(s) 478, 824, 1142
Ama87I CYCGRG 1 cut(s) 905
Aor13HI TCCGGA 1 cut(s) 1399
AoxI GGCC 2 cut(s) 641, 922
ApeKI GCWGC 1 cut(s) 1074
ApoI RAATTY 2 cut(s) 440, 1308
AseI ATTAAT 1 cut(s) 653
AspS9I GGNCC 4 cut(s) 215, 458, 552, 923
AsuHPI GGTGA 2 cut(s) 644, 1523
AvaI CYCGRG 1 cut(s) 905
AvaII GGWCC 3 cut(s) 215, 458, 552
BanI GGYRCC 2 cut(s) 178, 695
BarI GAAGNNNNNNTAC 2 cut(s) 10, 42
BauI CACGAG 2 cut(s) 618, 795
BbvI GCAGC 1 cut(s) 1061
BccI CCATC 5 cut(s) 389, 403, 841, 944, 1060
BciT130I CCWGG 2 cut(s) 290, 1286
BciVI GTATCC 1 cut(s) 1126
BclI TGATCA 1 cut(s) 681
BcoDI GTCTC 6 cut(s) 346, 376, 668, 792, 1254, 1401
BfaI CTAG 3 cut(s) 32, 575, 981
BfmI CTRYAG 1 cut(s) 1444
BfuI GTATCC 1 cut(s) 1126
BisI GCNGC 1 cut(s) 1075
BlsI GCNGC 1 cut(s) 1076
BmcAI AGTACT 1 cut(s) 62
Bme1390I CCNGG 2 cut(s) 290, 1286
Bme18I GGWCC 3 cut(s) 215, 458, 552
BmeT110I CYCGRG 1 cut(s) 905
BmgT120I GGNCC 4 cut(s) 215, 458, 552, 923
BmiI GGNNCC 2 cut(s) 180, 697
BmrFI CCNGG 2 cut(s) 290, 1286
BmrI ACTGGG 2 cut(s) 638, 920
BmsI GCATC 1 cut(s) 479
BmuI ACTGGG 2 cut(s) 638, 920
Bpu10I CCTNAGC 1 cut(s) 988
BsaBI GATNNNNATC 3 cut(s) 686, 1028, 1239
BsaI GGTCTC 1 cut(s) 1254
BsaJI CCNNGG 1 cut(s) 1410
BsaWI WCCGGW 1 cut(s) 1399
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bsc4I CCNNNNNNNGG 2 cut(s) 1385, 1416
Bse1I ACTGG 5 cut(s) 164, 314, 633, 926, 1356
Bse8I GATNNNNATC 3 cut(s) 686, 1028, 1239
BseAI TCCGGA 1 cut(s) 1399
BseBI CCWGG 2 cut(s) 290, 1286
BseDI CCNNGG 1 cut(s) 1410
BseGI GGATG 5 cut(s) 187, 1068, 1093, 1294, 1504
BseJI GATNNNNATC 3 cut(s) 686, 1028, 1239
BseLI CCNNNNNNNGG 2 cut(s) 1385, 1416
BseMII CTCAG 2 cut(s) 851, 1002
BseNI ACTGG 5 cut(s) 164, 314, 633, 926, 1356
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 1 cut(s) 1061
BshFI GGCC 2 cut(s) 643, 924
BshNI GGYRCC 2 cut(s) 178, 695
BsiHKCI CYCGRG 1 cut(s) 905
BsiSI CCGG 1 cut(s) 1400
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 2 cut(s) 1385, 1416
BsmAI GTCTC 6 cut(s) 346, 376, 668, 792, 1254, 1401
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 2 cut(s) 643, 924
Bso31I GGTCTC 1 cut(s) 1254
BsoBI CYCGRG 1 cut(s) 905
Bsp13I TCCGGA 1 cut(s) 1399
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 6 cut(s) 483, 681, 829, 846, 1029, 1147
Bsp19I CCATGG 1 cut(s) 1410
BspANI GGCC 2 cut(s) 643, 924
BspCNI CTCAG 2 cut(s) 850, 1001
BspEI TCCGGA 1 cut(s) 1399
BspLI GGNNCC 2 cut(s) 180, 697
BspPI GGATC 3 cut(s) 478, 824, 1142
BspT107I GGYRCC 2 cut(s) 178, 695
BspTNI GGTCTC 1 cut(s) 1254
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 5 cut(s) 164, 314, 633, 926, 1356
BssECI CCNNGG 1 cut(s) 1410
BssMI GATC 6 cut(s) 483, 681, 829, 846, 1029, 1147
BssNAI GTATAC 1 cut(s) 941
BssSI CACGAG 2 cut(s) 618, 795
BssT1I CCWWGG 1 cut(s) 1410
Bst1107I GTATAC 1 cut(s) 941
Bst2BI CACGAG 2 cut(s) 618, 795
Bst2UI CCWGG 2 cut(s) 290, 1286
Bst4CI ACNGT 3 cut(s) 503, 751, 1576
Bst6I CTCTTC 2 cut(s) 118, 999
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 3 cut(s) 641, 726, 1330
BstDEI CTNAG 3 cut(s) 129, 837, 988
BstDSI CCRYGG 1 cut(s) 1410
BstF5I GGATG 5 cut(s) 187, 1068, 1093, 1294, 1504
BstKTI GATC 6 cut(s) 486, 684, 832, 849, 1032, 1150
BstMAI GTCTC 6 cut(s) 346, 376, 668, 792, 1254, 1401
BstMBI GATC 6 cut(s) 483, 681, 829, 846, 1029, 1147
BstNI CCWGG 2 cut(s) 290, 1286
BstSCI CCNGG 2 cut(s) 288, 1284
BstSFI CTRYAG 1 cut(s) 1444
BstV1I GCAGC 1 cut(s) 1061
BstX2I RGATCY 1 cut(s) 483
BstYI RGATCY 1 cut(s) 483
BstZ17I GTATAC 1 cut(s) 941
BsuI GTATCC 1 cut(s) 1126
BsuRI GGCC 2 cut(s) 643, 924
BtgI CCRYGG 1 cut(s) 1410
BtsCI GGATG 5 cut(s) 187, 1068, 1093, 1294, 1504
BtsIMutI CAGTG 1 cut(s) 1581
Cac8I GCNNGC 3 cut(s) 641, 726, 1330
Cfr13I GGNCC 4 cut(s) 215, 458, 552, 923
CseI GACGC 1 cut(s) 1191
Csp6I GTAC 4 cut(s) 61, 123, 425, 715
CviAII CATG 5 cut(s) 192, 437, 1114, 1411, 1458
CviQI GTAC 4 cut(s) 61, 123, 425, 715
DdeI CTNAG 3 cut(s) 129, 837, 988
DpnI GATC 6 cut(s) 485, 683, 831, 848, 1031, 1149
DpnII GATC 6 cut(s) 483, 681, 829, 846, 1029, 1147
DrdI GACNNNNNNGTC 1 cut(s) 1268
DseDI GACNNNNNNGTC 1 cut(s) 1268
Eam1104I CTCTTC 2 cut(s) 118, 999
EarI CTCTTC 2 cut(s) 118, 999
Eco130I CCWWGG 1 cut(s) 1410
Eco31I GGTCTC 1 cut(s) 1254
Eco47I GGWCC 3 cut(s) 215, 458, 552
Eco57I CTGAAG 2 cut(s) 99, 1265
Eco88I CYCGRG 1 cut(s) 905
EcoRI GAATTC 1 cut(s) 440
EcoRII CCWGG 2 cut(s) 288, 1284
EcoT14I CCWWGG 1 cut(s) 1410
EcoT22I ATGCAT 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 1410
FaeI CATG 5 cut(s) 195, 440, 1117, 1414, 1461
FaqI GGGAC 1 cut(s) 268
FatI CATG 5 cut(s) 191, 436, 1113, 1410, 1457
FbaI TGATCA 1 cut(s) 681
FblI GTMKAC 2 cut(s) 940, 1443
Fnu4HI GCNGC 1 cut(s) 1075
FokI GGATG 5 cut(s) 174, 1075, 1100, 1301, 1511
Fsp4HI GCNGC 1 cut(s) 1075
FspBI CTAG 3 cut(s) 32, 575, 981
GluI GCNGC 1 cut(s) 1075
HaeIII GGCC 2 cut(s) 643, 924
HapII CCGG 1 cut(s) 1400
HgaI GACGC 1 cut(s) 1191
Hin1II CATG 5 cut(s) 195, 440, 1117, 1414, 1461
HindIII AAGCTT 2 cut(s) 234, 1126
HinfI GANTC 6 cut(s) 4, 163, 242, 784, 1235, 1514
HpaII CCGG 1 cut(s) 1400
HphI GGTGA 2 cut(s) 644, 1523
Hpy166II GTNNAC 4 cut(s) 27, 215, 941, 1444
Hpy188III TCNNGA 7 cut(s) 32, 378, 461, 795, 990, 1012, 1400
Hpy8I GTNNAC 4 cut(s) 27, 215, 941, 1444
HpyAV CCTTC 7 cut(s) 113, 629, 949, 1240, 1334, 1469, 1577
HpyCH4III ACNGT 3 cut(s) 503, 751, 1576
HpyCH4IV ACGT 1 cut(s) 1136
HpyCH4V TGCA 8 cut(s) 231, 255, 436, 480, 521, 612, 896, 1077
HpyF3I CTNAG 3 cut(s) 129, 837, 988
HpySE526I ACGT 1 cut(s) 1136
Hsp92II CATG 5 cut(s) 195, 440, 1117, 1414, 1461
Kpn2I TCCGGA 1 cut(s) 1399
Ksp22I TGATCA 1 cut(s) 681
Kzo9I GATC 6 cut(s) 483, 681, 829, 846, 1029, 1147
LmnI GCTCC 2 cut(s) 46, 297
Lsp1109I GCAGC 1 cut(s) 1061
LweI GCATC 1 cut(s) 479
MaeI CTAG 3 cut(s) 32, 575, 981
MaeII ACGT 1 cut(s) 1136
MaeIII GTNAC 2 cut(s) 194, 1095
MalI GATC 6 cut(s) 485, 683, 831, 848, 1031, 1149
MboI GATC 6 cut(s) 483, 681, 829, 846, 1029, 1147
MboII GAAGA 7 cut(s) 105, 116, 401, 418, 576, 1016, 1031
MflI RGATCY 1 cut(s) 483
MluCI AATT 6 cut(s) 338, 440, 654, 883, 1308, 1546
Mph1103I ATGCAT 1 cut(s) 523
MroI TCCGGA 1 cut(s) 1399
MseI TTAA 7 cut(s) 65, 147, 528, 653, 806, 1080, 1188
MslI CAYNNNNRTG 3 cut(s) 192, 1367, 1574
MspI CCGG 1 cut(s) 1400
MspR9I CCNGG 2 cut(s) 290, 1286
MvaI CCWGG 2 cut(s) 290, 1286
NcoI CCATGG 1 cut(s) 1410
NdeII GATC 6 cut(s) 483, 681, 829, 846, 1029, 1147
NlaIII CATG 5 cut(s) 195, 440, 1117, 1414, 1461
NlaIV GGNNCC 2 cut(s) 180, 697
NmuCI GTSAC 1 cut(s) 194
NsiI ATGCAT 1 cut(s) 523
PfeI GAWTC 6 cut(s) 4, 163, 242, 784, 1235, 1514
PflMI CCANNNNNTGG 1 cut(s) 1385
PfoI TCCNGGA 1 cut(s) 1284
PkrI GCNGC 1 cut(s) 1076
PshBI ATTAAT 1 cut(s) 653
PsiI TTATAA 1 cut(s) 933
Psp6I CCWGG 2 cut(s) 288, 1284
PspGI CCWGG 2 cut(s) 288, 1284
PspN4I GGNNCC 2 cut(s) 180, 697
PspPI GGNCC 4 cut(s) 215, 458, 552, 923
PsuI RGATCY 1 cut(s) 483
RsaI GTAC 4 cut(s) 62, 124, 426, 716
RsaNI GTAC 4 cut(s) 61, 123, 425, 715
RseI CAYNNNNRTG 3 cut(s) 192, 1367, 1574
SaqAI TTAA 7 cut(s) 65, 147, 528, 653, 806, 1080, 1188
SatI GCNGC 1 cut(s) 1075
Sau3AI GATC 6 cut(s) 483, 681, 829, 846, 1029, 1147
Sau96I GGNCC 4 cut(s) 215, 458, 552, 923
ScaI AGTACT 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 290, 1286
SfaNI GCATC 1 cut(s) 479
SfcI CTRYAG 1 cut(s) 1444
SinI GGWCC 3 cut(s) 215, 458, 552
SmiMI CAYNNNNRTG 3 cut(s) 192, 1367, 1574
Sse9I AATT 6 cut(s) 338, 440, 654, 883, 1308, 1546
SspI AATATT 1 cut(s) 1192
SspMI CTAG 3 cut(s) 32, 575, 981
StyD4I CCNGG 2 cut(s) 288, 1284
StyI CCWWGG 1 cut(s) 1410
TaaI ACNGT 3 cut(s) 503, 751, 1576
TaiI ACGT 1 cut(s) 1139
TaqI TCGA 3 cut(s) 16, 377, 1146
TaqII GACCGA 1 cut(s) 446
TasI AATT 6 cut(s) 338, 440, 654, 883, 1308, 1546
TatI WGTACW 2 cut(s) 60, 714
TfiI GAWTC 6 cut(s) 4, 163, 242, 784, 1235, 1514
Tru1I TTAA 7 cut(s) 65, 147, 528, 653, 806, 1080, 1188
Tru9I TTAA 7 cut(s) 65, 147, 528, 653, 806, 1080, 1188
TscAI CASTG 1 cut(s) 1581
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 1 cut(s) 1074
Tsp45I GTSAC 1 cut(s) 194
TspRI CASTG 1 cut(s) 1581
Van91I CCANNNNNTGG 1 cut(s) 1385
VpaK11BI GGWCC 3 cut(s) 215, 458, 552
VspI ATTAAT 1 cut(s) 653
XapI RAATTY 2 cut(s) 440, 1308
XbaI TCTAGA 1 cut(s) 31
XmiI GTMKAC 2 cut(s) 940, 1443
XspI CTAG 3 cut(s) 32, 575, 981
ZrmI AGTACT 1 cut(s) 62
Zsp2I ATGCAT 1 cut(s) 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.