MD05G1264700.v1.1

Protein of unknown function (DUF640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014900
Physical Location & Seq
Forward (+)
39978908 .. 39979825
918 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1264700.v1.1.491

Sequence Viewer

Length: 918 bp
ATGGGACCCTCCTTTTGTTTCCTTCCCCCATCTTCTCTCTTCTCATTGAAATTATGTCCCCCCACTTCCTCTCCTCTCCTTCTTTCCCTCCCCGGCCCCTACCCACTCTTTATATATTCCTACACGTCTTTCTACCGTCTCTCATACATTTCACACATAGCACTTTGTCCTCATATCATCATACAATTTAATCGTTTTGAAAGCCATCAAACTTTGTGTACCATGTCAGCAGCAGCCGCAGCGTCCGCGGCGGCGGCACTGAGTAATTCGAGCTCCACTAACAGCAACTCCATCACCGATAACTACCACCACCAACCGTTATTCACACCGTCCCCGCTAGCGGCCGTGACGCCAGTGCTGAGCAGGTACGAGTCCCAGAAGCGGCGGGACTGGATCACGTTCGGGCAGTACCTCAAGAACCACCGCCCGCCGCTTAACCTGTCCCGCTGCAGTGGGGCGCACGTGCTGGAGTTCCTTAGGTATTTGGATCAGTTCGGGAAGACCAAGGTCCACGCGGACGCTTGCCCTTTCTACGGGCACCCTAACCCTCCTGCGCCCTGCCCATGCCCGCTGCGGCAGGCTTGGGGCAGCCTGGACGCTCTCATTGGGCGGCTTCGGGCAGCGTTTGAGGAGAACGGCGGGCACCCCGAGACTAACCCGTTTGGGGCACGGGCAGTGAGGCTGTACCTGAGGGAGGTGAGGGACAGCCAGGCCAGGGCAAGGGGGATTGCTTATGAGAAGAAGAAGAGAAAGAAAGTAGGGCCGTCACAACAGCTGCAGGGGAATGATCAACAACATGATGAGGAGGAGAGGCATGGGCAACATGGTAATTTTGGTTCAGATTATGGGTATAGTGCTGCTGGGGCTTCATCCGCTACTGCCAGGGACTCCATGATGCCCTTATCTGTGCTAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF640

Protein Analysis

306

Amino Acids

33.71

Weight (kDa)

9.18

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALOG_dom PF04852 117 - 235 4.1e-65 ALOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014900)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11120
malus_domestica MD05G1264700.v1.1 MD10G1242600.v1.1
prunus_persica Prupe.4G101300_v2.0.a1
pyrus_communis pycom05g24860 pycom10g20310
rosa_chinensis RchiOBHm_Chr5g0018011
rosa_laevigata RLG00000032333
rosa_multiflora Rmu_sc0010280.1_g000005
rosa_roxburghii Rroxscaffold_1G00059600
rosa_rugosa Rorug05G0039600
rosa_samantha Rh5AG132500 Rh5BG130700 Rh5CG143000 Rh5DG132700
rosa_wichuraiana Rw5G011540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 354
AccB1I GGYRCC 2 cut(s) 537, 642
AccII CGCG 2 cut(s) 248, 515
AclWI GGATC 2 cut(s) 401, 495
AcoI YGGCCR 1 cut(s) 342
AcvI CACGTG 1 cut(s) 463
AcyI GRCGYC 1 cut(s) 350
AfaI GTAC 4 cut(s) 220, 368, 410, 686
AfiI CCNNNNNNNGG 7 cut(s) 340, 381, 533, 664, 694, 715, 720
AflIII ACRYGT 1 cut(s) 123
AgsI TTSAA 2 cut(s) 49, 200
AjiI CACGTC 1 cut(s) 126
AjnI CCWGG 4 cut(s) 591, 708, 713, 881
AluBI AGCT 2 cut(s) 273, 775
AluI AGCT 2 cut(s) 273, 775
Alw21I GWGCWC 1 cut(s) 275
Alw26I GTCTC 2 cut(s) 143, 644
AlwI GGATC 2 cut(s) 401, 495
Ama87I CYCGRG 1 cut(s) 647
AoxI GGCC 4 cut(s) 94, 342, 711, 761
ApeKI GCWGC 9 cut(s) 230, 233, 239, 447, 571, 588, 620, 775, 857
ArsI GACNNNNNNTTYG 1 cut(s) 29
AspLEI GCGC 2 cut(s) 460, 556
AspS9I GGNCC 4 cut(s) 5, 95, 508, 761
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 2 cut(s) 286, 709
AsuNHI GCTAGC 1 cut(s) 337
AvaI CYCGRG 1 cut(s) 647
AvaII GGWCC 2 cut(s) 5, 508
AxyI CCTNAGG 2 cut(s) 476, 689
BaeGI GKGCMC 3 cut(s) 540, 645, 670
BanI GGYRCC 2 cut(s) 537, 642
BanII GRGCYC 1 cut(s) 275
BbrPI CACGTG 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 275
BbvI GCAGC 9 cut(s) 242, 245, 251, 434, 558, 600, 632, 762, 844
BccI CCATC 3 cut(s) 37, 213, 299
BceAI ACGGC 3 cut(s) 329, 652, 748
BciT130I CCWGG 4 cut(s) 593, 710, 715, 883
BclI TGATCA 1 cut(s) 787
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 2 cut(s) 143, 644
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 2 cut(s) 448, 776
BfuAI ACCTGC 1 cut(s) 354
BlpI GCTNAGC 1 cut(s) 359
Bme1390I CCNGG 5 cut(s) 93, 593, 710, 715, 883
Bme18I GGWCC 2 cut(s) 5, 508
BmeT110I CYCGRG 1 cut(s) 647
BmgBI CACGTC 1 cut(s) 126
BmgT120I GGNCC 4 cut(s) 5, 95, 508, 761
BmiI GGNNCC 5 cut(s) 6, 7, 97, 539, 644
BmrFI CCNGG 5 cut(s) 93, 593, 710, 715, 883
BmsI GCATC 1 cut(s) 885
BmtI GCTAGC 1 cut(s) 341
BoxI GACNNNNGTC 1 cut(s) 506
BpiI GAAGAC 1 cut(s) 506
BpmI CTGGAG 1 cut(s) 488
Bpu1102I GCTNAGC 1 cut(s) 359
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 93
BsaAI YACGTR 1 cut(s) 463
BsaHI GRCGYC 1 cut(s) 350
BsaJI CCNNGG 5 cut(s) 91, 246, 504, 714, 882
BsaXI ACNNNNNCTCC 4 cut(s) 55, 85, 272, 302
Bsc4I CCNNNNNNNGG 7 cut(s) 340, 381, 533, 664, 694, 715, 720
Bse1I ACTGG 2 cut(s) 353, 395
Bse21I CCTNAGG 2 cut(s) 476, 689
BseBI CCWGG 4 cut(s) 593, 710, 715, 883
BseDI CCNNGG 5 cut(s) 91, 246, 504, 714, 882
BseGI GGATG 1 cut(s) 869
BseLI CCNNNNNNNGG 7 cut(s) 340, 381, 533, 664, 694, 715, 720
BseMII CTCAG 3 cut(s) 251, 350, 680
BseNI ACTGG 2 cut(s) 353, 395
BseRI GAGGAG 4 cut(s) 63, 644, 818, 821
BseSI GKGCMC 3 cut(s) 540, 645, 670
BseX3I CGGCCG 1 cut(s) 342
BseXI GCAGC 9 cut(s) 242, 245, 251, 434, 558, 600, 632, 762, 844
BseYI CCCAGC 1 cut(s) 860
Bsh1236I CGCG 2 cut(s) 248, 515
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 4 cut(s) 96, 344, 713, 763
BshNI GGYRCC 2 cut(s) 537, 642
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 275
BsiHKCI CYCGRG 1 cut(s) 647
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 8 cut(s) 18, 42, 316, 358, 401, 427, 716, 899
BslI CCNNNNNNNGG 7 cut(s) 340, 381, 533, 664, 694, 715, 720
BsmAI GTCTC 2 cut(s) 143, 644
BsmBI CGTCTC 1 cut(s) 143
BsmFI GGGAC 8 cut(s) 18, 42, 316, 358, 401, 427, 716, 899
BsnI GGCC 4 cut(s) 96, 344, 713, 763
BsoBI CYCGRG 1 cut(s) 647
Bsp1286I GDGCHC 4 cut(s) 275, 540, 645, 670
Bsp143I GATC 3 cut(s) 393, 487, 787
Bsp1720I GCTNAGC 1 cut(s) 359
BspANI GGCC 4 cut(s) 96, 344, 713, 763
BspCNI CTCAG 3 cut(s) 252, 351, 681
BspFNI CGCG 2 cut(s) 248, 515
BspLI GGNNCC 5 cut(s) 6, 7, 97, 539, 644
BspMAI CTGCAG 2 cut(s) 452, 780
BspMI ACCTGC 1 cut(s) 354
BspOI GCTAGC 1 cut(s) 341
BspPI GGATC 2 cut(s) 401, 495
BspT107I GGYRCC 2 cut(s) 537, 642
BsrI ACTGG 2 cut(s) 353, 395
BssECI CCNNGG 5 cut(s) 91, 246, 504, 714, 882
BssMI GATC 3 cut(s) 393, 487, 787
BssNI GRCGYC 1 cut(s) 350
BssT1I CCWWGG 1 cut(s) 504
Bst2UI CCWGG 4 cut(s) 593, 710, 715, 883
Bst4CI ACNGT 3 cut(s) 137, 318, 330
Bst6I CTCTTC 2 cut(s) 44, 740
BstACI GRCGYC 1 cut(s) 350
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 6 cut(s) 339, 428, 523, 569, 579, 641
BstDEI CTNAG 4 cut(s) 260, 359, 476, 689
BstDSI CCRYGG 1 cut(s) 246
BstENI CCTNNNNNAGG 1 cut(s) 692
BstF5I GGATG 1 cut(s) 869
BstFNI CGCG 2 cut(s) 248, 515
BstHHI GCGC 2 cut(s) 460, 556
BstKTI GATC 3 cut(s) 396, 490, 790
BstMAI GTCTC 2 cut(s) 143, 644
BstMBI GATC 3 cut(s) 393, 487, 787
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 7 cut(s) 236, 239, 245, 248, 254, 863, 872
BstNI CCWGG 4 cut(s) 593, 710, 715, 883
BstPAI GACNNNNGTC 1 cut(s) 506
BstSCI CCNGG 5 cut(s) 91, 591, 708, 713, 881
BstSFI CTRYAG 2 cut(s) 448, 776
BstSLI GKGCMC 3 cut(s) 540, 645, 670
BstUI CGCG 2 cut(s) 248, 515
BstV1I GCAGC 9 cut(s) 242, 245, 251, 434, 558, 600, 632, 762, 844
BstV2I GAAGAC 1 cut(s) 506
BstZI CGGCCG 1 cut(s) 342
Bsu36I CCTNAGG 2 cut(s) 476, 689
BsuRI GGCC 4 cut(s) 96, 344, 713, 763
BtgI CCRYGG 1 cut(s) 246
BtrI CACGTC 1 cut(s) 126
BtsCI GGATG 1 cut(s) 869
BtsI GCAGTG 2 cut(s) 457, 681
BtsIMutI CAGTG 4 cut(s) 257, 360, 457, 681
BveI ACCTGC 1 cut(s) 354
Cac8I GCNNGC 6 cut(s) 339, 428, 523, 569, 579, 641
CfoI GCGC 2 cut(s) 460, 556
Cfr13I GGNCC 4 cut(s) 5, 95, 508, 761
Cfr42I CCGCGG 1 cut(s) 249
CseI GACGC 4 cut(s) 231, 358, 527, 605
Csp6I GTAC 4 cut(s) 219, 367, 409, 685
CviAII CATG 6 cut(s) 223, 564, 797, 815, 824, 892
CviQI GTAC 4 cut(s) 219, 367, 409, 685
DdeI CTNAG 4 cut(s) 260, 359, 476, 689
DpnI GATC 3 cut(s) 395, 489, 789
DpnII GATC 3 cut(s) 393, 487, 787
EaeI YGGCCR 1 cut(s) 342
EagI CGGCCG 1 cut(s) 342
Eam1104I CTCTTC 2 cut(s) 44, 740
EarI CTCTTC 2 cut(s) 44, 740
Ecl136II GAGCTC 1 cut(s) 273
EclXI CGGCCG 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 504
Eco24I GRGCYC 1 cut(s) 275
Eco47I GGWCC 2 cut(s) 5, 508
Eco52I CGGCCG 1 cut(s) 342
Eco53kI GAGCTC 1 cut(s) 273
Eco72I CACGTG 1 cut(s) 463
Eco81I CCTNAGG 2 cut(s) 476, 689
Eco88I CYCGRG 1 cut(s) 647
EcoICRI GAGCTC 1 cut(s) 273
EcoNI CCTNNNNNAGG 1 cut(s) 692
EcoO109I RGGNCCY 1 cut(s) 5
EcoRII CCWGG 4 cut(s) 591, 708, 713, 881
EcoT14I CCWWGG 1 cut(s) 504
EcoT38I GRGCYC 1 cut(s) 275
ErhI CCWWGG 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 143
FaeI CATG 6 cut(s) 226, 567, 800, 818, 827, 895
FaqI GGGAC 8 cut(s) 18, 42, 316, 358, 401, 427, 716, 899
FatI CATG 6 cut(s) 222, 563, 796, 814, 823, 891
FauI CCCGC 6 cut(s) 342, 378, 435, 452, 576, 632
FbaI TGATCA 1 cut(s) 787
FokI GGATG 1 cut(s) 856
FriOI GRGCYC 1 cut(s) 275
FspBI CTAG 1 cut(s) 338
GlaI GCGC 2 cut(s) 459, 555
GsaI CCCAGC 1 cut(s) 864
GsuI CTGGAG 1 cut(s) 488
HaeIII GGCC 4 cut(s) 96, 344, 713, 763
HapII CCGG 1 cut(s) 93
HgaI GACGC 4 cut(s) 231, 358, 527, 605
HhaI GCGC 2 cut(s) 460, 556
Hin1I GRCGYC 1 cut(s) 350
Hin1II CATG 6 cut(s) 226, 567, 800, 818, 827, 895
Hin6I GCGC 2 cut(s) 458, 554
HinP1I GCGC 2 cut(s) 458, 554
HinfI GANTC 2 cut(s) 371, 887
HpaII CCGG 1 cut(s) 93
HphI GGTGA 2 cut(s) 286, 709
Hpy166II GTNNAC 2 cut(s) 219, 511
Hpy188I TCNGA 2 cut(s) 841, 917
Hpy188III TCNNGA 2 cut(s) 415, 496
Hpy8I GTNNAC 2 cut(s) 219, 511
HpyAV CCTTC 2 cut(s) 32, 89
HpyCH4III ACNGT 3 cut(s) 137, 318, 330
HpyCH4IV ACGT 3 cut(s) 125, 398, 462
HpyCH4V TGCA 2 cut(s) 450, 778
HpyF10VI GCNNNNNNNGC 7 cut(s) 236, 239, 245, 248, 254, 863, 872
HpyF3I CTNAG 4 cut(s) 260, 359, 476, 689
HpySE526I ACGT 3 cut(s) 125, 398, 462
Hsp92I GRCGYC 1 cut(s) 350
Hsp92II CATG 6 cut(s) 226, 567, 800, 818, 827, 895
HspAI GCGC 2 cut(s) 458, 554
KflI GGGWCCC 1 cut(s) 5
Ksp22I TGATCA 1 cut(s) 787
KspI CCGCGG 1 cut(s) 249
Kzo9I GATC 3 cut(s) 393, 487, 787
LmnI GCTCC 1 cut(s) 278
Lsp1109I GCAGC 9 cut(s) 242, 245, 251, 434, 558, 600, 632, 762, 844
LweI GCATC 1 cut(s) 885
MaeI CTAG 1 cut(s) 338
MaeII ACGT 3 cut(s) 125, 398, 462
MaeIII GTNAC 2 cut(s) 346, 765
MalI GATC 3 cut(s) 395, 489, 789
MboI GATC 3 cut(s) 393, 487, 787
MboII GAAGA 6 cut(s) 24, 31, 511, 751, 754, 757
MhlI GDGCHC 4 cut(s) 275, 540, 645, 670
MluCI AATT 4 cut(s) 50, 185, 265, 829
MlyI GAGTC 2 cut(s) 380, 881
MseI TTAA 2 cut(s) 189, 435
MspA1I CMGCKG 4 cut(s) 248, 447, 571, 775
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 5 cut(s) 93, 593, 710, 715, 883
MvaI CCWGG 4 cut(s) 593, 710, 715, 883
MvnI CGCG 2 cut(s) 248, 515
MwoI GCNNNNNNNGC 7 cut(s) 236, 239, 245, 248, 254, 863, 872
NciI CCSGG 1 cut(s) 93
NdeII GATC 3 cut(s) 393, 487, 787
NheI GCTAGC 1 cut(s) 337
NlaIII CATG 6 cut(s) 226, 567, 800, 818, 827, 895
NlaIV GGNNCC 5 cut(s) 6, 7, 97, 539, 644
NmuCI GTSAC 2 cut(s) 346, 765
PleI GAGTC 2 cut(s) 379, 881
PmaCI CACGTG 1 cut(s) 463
PmlI CACGTG 1 cut(s) 463
PpsI GAGTC 2 cut(s) 379, 881
Ppu21I YACGTR 1 cut(s) 463
PpuMI RGGWCCY 1 cut(s) 5
PshAI GACNNNNGTC 1 cut(s) 506
Psp124BI GAGCTC 1 cut(s) 275
Psp5II RGGWCCY 1 cut(s) 5
Psp6I CCWGG 4 cut(s) 591, 708, 713, 881
PspCI CACGTG 1 cut(s) 463
PspFI CCCAGC 1 cut(s) 860
PspGI CCWGG 4 cut(s) 591, 708, 713, 881
PspN4I GGNNCC 5 cut(s) 6, 7, 97, 539, 644
PspPI GGNCC 4 cut(s) 5, 95, 508, 761
PspPPI RGGWCCY 1 cut(s) 5
PsrI GAACNNNNNNTAC 2 cut(s) 820, 852
PstI CTGCAG 2 cut(s) 452, 780
PvuII CAGCTG 1 cut(s) 775
RsaI GTAC 4 cut(s) 220, 368, 410, 686
RsaNI GTAC 4 cut(s) 219, 367, 409, 685
SacI GAGCTC 1 cut(s) 275
SacII CCGCGG 1 cut(s) 249
SaqAI TTAA 2 cut(s) 189, 435
Sau3AI GATC 3 cut(s) 393, 487, 787
Sau96I GGNCC 4 cut(s) 5, 95, 508, 761
SchI GAGTC 2 cut(s) 380, 881
ScrFI CCNGG 5 cut(s) 93, 593, 710, 715, 883
SduI GDGCHC 4 cut(s) 275, 540, 645, 670
SfaNI GCATC 1 cut(s) 885
SfcI CTRYAG 2 cut(s) 448, 776
Sfr303I CCGCGG 1 cut(s) 249
SgrBI CCGCGG 1 cut(s) 249
SinI GGWCC 2 cut(s) 5, 508
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 4 cut(s) 50, 185, 265, 829
SspMI CTAG 1 cut(s) 338
SstI GAGCTC 1 cut(s) 275
StyD4I CCNGG 5 cut(s) 91, 591, 708, 713, 881
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 3 cut(s) 137, 318, 330
TaiI ACGT 3 cut(s) 128, 401, 465
TaqI TCGA 1 cut(s) 269
TasI AATT 4 cut(s) 50, 185, 265, 829
TauI GCSGC 9 cut(s) 239, 251, 254, 257, 344, 385, 433, 577, 613
Tru1I TTAA 2 cut(s) 189, 435
Tru9I TTAA 2 cut(s) 189, 435
TscAI CASTG 4 cut(s) 264, 360, 457, 681
TseFI GTSAC 2 cut(s) 346, 765
TseI GCWGC 9 cut(s) 230, 233, 239, 447, 571, 588, 620, 775, 857
Tsp45I GTSAC 2 cut(s) 346, 765
TspDTI ATGAA 1 cut(s) 858
TspRI CASTG 4 cut(s) 264, 360, 457, 681
VpaK11BI GGWCC 2 cut(s) 5, 508
XagI CCTNNNNNAGG 1 cut(s) 692
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.