MD10G1242600.v1.1

Protein of unknown function (DUF640)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014900
Physical Location & Seq
Forward (+)
33749895 .. 33750630
736 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1242600.v1.1.491

Sequence Viewer

Length: 660 bp
ATGTCAGCAGCAGCCGCAGCCGCCGCCGCTGCGGCAGTGAGTAGGTCTAGCTCCACCAACAGTAATTCTATCACCTACAACTACCGCCACCAACCGTTATTCAAGCCGTCCTCGCTAACGGCCGTGGTGCCGGTGCTGAGCAGGTACGAGTCCCAGAAGCGGCGGGACTGGATCTCGTTTGGGCAGTACCTCAAGAACCACCGCCCGCCGCTGAACCTGTCCCGCTGCAGCGGGGCCCACGTGCTGGAGTTCCTTAGGTATTTGGATCAGTTCGGGAAGACTAAGGTCCATGCGGACGCTTGCCCTTTCTACGGGCACCCTAACCCTCCTGCACCCTGTCCGTGCCCGCTGCGGCAGGCCTGGGGCAGCCTCGACGCAATCATTGGAAGGCTTCGGGCAGCGTTTGAGGAGAACGGCGGGCAGCCCGAGACCAACCCGTTTGGGGCACGAGCAGTGAGGCTGTACCTGAAGGAGGTCAGGGACAGCCAGGCCAAGGCCAGGGGAATTGCTTATGAGAAGAAGAAGAGAAAGAAAGTAGGTCCATCACAACAGCCGGAAGGGCAAGATGATGTGGAGCAGCAGCAGGGGCAACATGGTACTTTTGGTTCAGATTATGGGTATGGTGCTGCTGTTACTTCATCTGCTGATGCTGCTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF640

Protein Analysis

220

Amino Acids

23.87

Weight (kDa)

9.56

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALOG_dom PF04852 44 - 161 2.6e-64 ALOG domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014900)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g11120
malus_domestica MD05G1264700.v1.1 MD10G1242600.v1.1
prunus_persica Prupe.4G101300_v2.0.a1
pyrus_communis pycom05g24860 pycom10g20310
rosa_chinensis RchiOBHm_Chr5g0018011
rosa_laevigata RLG00000032333
rosa_multiflora Rmu_sc0010280.1_g000005
rosa_roxburghii Rroxscaffold_1G00059600
rosa_rugosa Rorug05G0039600
rosa_samantha Rh5AG132500 Rh5BG130700 Rh5CG143000 Rh5DG132700
rosa_wichuraiana Rw5G011540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 132
AccB1I GGYRCC 2 cut(s) 127, 315
AccB7I CCANNNNNTGG 1 cut(s) 244
AclWI GGATC 2 cut(s) 179, 273
AcoI YGGCCR 1 cut(s) 120
AcuI CTGAAG 1 cut(s) 488
AcvI CACGTG 1 cut(s) 241
AfaI GTAC 4 cut(s) 146, 188, 464, 598
AfiI CCNNNNNNNGG 7 cut(s) 159, 244, 311, 442, 472, 493, 498
AgsI TTSAA 1 cut(s) 103
AjnI CCWGG 3 cut(s) 359, 486, 497
AluBI AGCT 1 cut(s) 51
AluI AGCT 1 cut(s) 51
Alw26I GTCTC 1 cut(s) 422
AlwI GGATC 2 cut(s) 179, 273
Ama87I CYCGRG 1 cut(s) 425
AoxI GGCC 5 cut(s) 120, 234, 357, 489, 495
ApaI GGGCCC 1 cut(s) 238
AspS9I GGNCC 4 cut(s) 234, 235, 286, 539
AsuHPI GGTGA 1 cut(s) 64
AvaI CYCGRG 1 cut(s) 425
AvaII GGWCC 2 cut(s) 286, 539
AxyI CCTNAGG 1 cut(s) 254
BaeGI GKGCMC 4 cut(s) 238, 318, 347, 448
BanI GGYRCC 2 cut(s) 127, 315
BanII GRGCYC 1 cut(s) 238
BauI CACGAG 1 cut(s) 447
BbrPI CACGTG 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 284
BccI CCATC 1 cut(s) 550
BceAI ACGGC 4 cut(s) 91, 107, 135, 430
BciT130I CCWGG 3 cut(s) 361, 488, 499
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 2 cut(s) 48, 654
BfmI CTRYAG 1 cut(s) 226
BfuAI ACCTGC 1 cut(s) 132
BglI GCCNNNNNGGC 2 cut(s) 32, 559
BlpI GCTNAGC 1 cut(s) 137
Bme1390I CCNGG 3 cut(s) 361, 488, 499
Bme18I GGWCC 2 cut(s) 286, 539
BmeT110I CYCGRG 1 cut(s) 425
BmgT120I GGNCC 4 cut(s) 234, 235, 286, 539
BmiI GGNNCC 4 cut(s) 129, 235, 236, 317
BmrFI CCNGG 3 cut(s) 361, 488, 499
BmsI GCATC 1 cut(s) 637
BoxI GACNNNNGTC 1 cut(s) 284
BpiI GAAGAC 1 cut(s) 284
BpmI CTGGAG 1 cut(s) 266
Bpu1102I GCTNAGC 1 cut(s) 137
BpuEI CTTGAG 1 cut(s) 176
BsaAI YACGTR 1 cut(s) 241
BsaI GGTCTC 1 cut(s) 422
BsaJI CCNNGG 4 cut(s) 123, 360, 492, 498
Bsc4I CCNNNNNNNGG 7 cut(s) 159, 244, 311, 442, 472, 493, 498
Bse118I RCCGGY 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 173
Bse21I CCTNAGG 1 cut(s) 254
BseBI CCWGG 3 cut(s) 361, 488, 499
BseDI CCNNGG 4 cut(s) 123, 360, 492, 498
BseLI CCNNNNNNNGG 7 cut(s) 159, 244, 311, 442, 472, 493, 498
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 173
BseRI GAGGAG 1 cut(s) 422
BseSI GKGCMC 4 cut(s) 238, 318, 347, 448
BseX3I CGGCCG 1 cut(s) 120
BsgI GTGCAG 1 cut(s) 315
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 5 cut(s) 122, 236, 359, 491, 497
BshNI GGYRCC 2 cut(s) 127, 315
BsiEI CGRYCG 1 cut(s) 123
BsiHKCI CYCGRG 1 cut(s) 425
BsiSI CCGG 2 cut(s) 131, 554
BslFI GGGAC 4 cut(s) 136, 179, 205, 494
BslI CCNNNNNNNGG 7 cut(s) 159, 244, 311, 442, 472, 493, 498
BsmAI GTCTC 1 cut(s) 422
BsmFI GGGAC 4 cut(s) 136, 179, 205, 494
BsnI GGCC 5 cut(s) 122, 236, 359, 491, 497
Bso31I GGTCTC 1 cut(s) 422
BsoBI CYCGRG 1 cut(s) 425
Bsp120I GGGCCC 1 cut(s) 234
Bsp1286I GDGCHC 4 cut(s) 238, 318, 347, 448
Bsp143I GATC 2 cut(s) 171, 265
Bsp1720I GCTNAGC 1 cut(s) 137
BspANI GGCC 5 cut(s) 122, 236, 359, 491, 497
BspCNI CTCAG 1 cut(s) 129
BspLI GGNNCC 4 cut(s) 129, 235, 236, 317
BspMAI CTGCAG 1 cut(s) 230
BspMI ACCTGC 1 cut(s) 132
BspPI GGATC 2 cut(s) 179, 273
BspT107I GGYRCC 2 cut(s) 127, 315
BspTNI GGTCTC 1 cut(s) 422
BsrFI RCCGGY 1 cut(s) 130
BsrI ACTGG 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 130
BssECI CCNNGG 4 cut(s) 123, 360, 492, 498
BssMI GATC 2 cut(s) 171, 265
BssSI CACGAG 1 cut(s) 447
BssT1I CCWWGG 1 cut(s) 492
Bst2BI CACGAG 1 cut(s) 447
Bst2UI CCWGG 3 cut(s) 361, 488, 499
Bst4CI ACNGT 2 cut(s) 62, 96
Bst6I CTCTTC 1 cut(s) 518
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 5 cut(s) 206, 301, 347, 357, 419
BstDEI CTNAG 3 cut(s) 137, 254, 282
BstDSI CCRYGG 1 cut(s) 123
BstENI CCTNNNNNAGG 1 cut(s) 470
BstKTI GATC 2 cut(s) 174, 268
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 2 cut(s) 171, 265
BstMCI CGRYCG 1 cut(s) 123
BstNI CCWGG 3 cut(s) 361, 488, 499
BstPAI GACNNNNGTC 1 cut(s) 284
BstSCI CCNGG 3 cut(s) 359, 486, 497
BstSFI CTRYAG 1 cut(s) 226
BstSLI GKGCMC 4 cut(s) 238, 318, 347, 448
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 171
BstYI RGATCY 1 cut(s) 171
BstZI CGGCCG 1 cut(s) 120
Bsu36I CCTNAGG 1 cut(s) 254
BsuRI GGCC 5 cut(s) 122, 236, 359, 491, 497
BtgI CCRYGG 1 cut(s) 123
BtsI GCAGTG 2 cut(s) 42, 459
BtsIMutI CAGTG 2 cut(s) 42, 459
BveI ACCTGC 1 cut(s) 132
Cac8I GCNNGC 5 cut(s) 206, 301, 347, 357, 419
Cfr10I RCCGGY 1 cut(s) 130
Cfr13I GGNCC 4 cut(s) 234, 235, 286, 539
CseI GACGC 2 cut(s) 305, 383
Csp6I GTAC 4 cut(s) 145, 187, 463, 597
CviAII CATG 2 cut(s) 290, 593
CviQI GTAC 4 cut(s) 145, 187, 463, 597
DdeI CTNAG 3 cut(s) 137, 254, 282
DpnI GATC 2 cut(s) 173, 267
DpnII GATC 2 cut(s) 171, 265
EaeI YGGCCR 1 cut(s) 120
EagI CGGCCG 1 cut(s) 120
Eam1104I CTCTTC 1 cut(s) 518
EarI CTCTTC 1 cut(s) 518
EclXI CGGCCG 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 492
Eco147I AGGCCT 1 cut(s) 359
Eco24I GRGCYC 1 cut(s) 238
Eco31I GGTCTC 1 cut(s) 422
Eco47I GGWCC 2 cut(s) 286, 539
Eco52I CGGCCG 1 cut(s) 120
Eco57I CTGAAG 1 cut(s) 488
Eco72I CACGTG 1 cut(s) 241
Eco81I CCTNAGG 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 425
EcoNI CCTNNNNNAGG 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 234
EcoRII CCWGG 3 cut(s) 359, 486, 497
EcoT14I CCWWGG 1 cut(s) 492
EcoT38I GRGCYC 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 2 cut(s) 293, 596
FaiI YATR 5 cut(s) 291, 513, 594, 615, 621
FaqI GGGAC 4 cut(s) 136, 179, 205, 494
FatI CATG 2 cut(s) 289, 592
FauI CCCGC 6 cut(s) 156, 213, 224, 230, 354, 410
FriOI GRGCYC 1 cut(s) 238
FspBI CTAG 2 cut(s) 48, 654
GsuI CTGGAG 1 cut(s) 266
HaeIII GGCC 5 cut(s) 122, 236, 359, 491, 497
HapII CCGG 2 cut(s) 131, 554
HgaI GACGC 2 cut(s) 305, 383
Hin1II CATG 2 cut(s) 293, 596
HinfI GANTC 1 cut(s) 149
HpaII CCGG 2 cut(s) 131, 554
HphI GGTGA 1 cut(s) 64
Hpy188I TCNGA 1 cut(s) 610
Hpy188III TCNNGA 2 cut(s) 193, 274
Hpy99I CGWCG 1 cut(s) 377
HpyAV CCTTC 3 cut(s) 381, 463, 551
HpyCH4III ACNGT 2 cut(s) 62, 96
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 2 cut(s) 228, 332
HpyF3I CTNAG 3 cut(s) 137, 254, 282
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 2 cut(s) 293, 596
Kzo9I GATC 2 cut(s) 171, 265
LmnI GCTCC 2 cut(s) 56, 574
LweI GCATC 1 cut(s) 637
MaeI CTAG 2 cut(s) 48, 654
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 631
MalI GATC 2 cut(s) 173, 267
MboI GATC 2 cut(s) 171, 265
MboII GAAGA 4 cut(s) 289, 529, 532, 535
MflI RGATCY 1 cut(s) 171
MhlI GDGCHC 4 cut(s) 238, 318, 347, 448
MluCI AATT 2 cut(s) 64, 504
MlyI GAGTC 1 cut(s) 158
MnlI CCTC 7 cut(s) 121, 200, 336, 380, 400, 450, 466
MspA1I CMGCKG 5 cut(s) 29, 211, 225, 231, 349
MspI CCGG 2 cut(s) 131, 554
MspR9I CCNGG 3 cut(s) 361, 488, 499
MvaI CCWGG 3 cut(s) 361, 488, 499
NdeII GATC 2 cut(s) 171, 265
NlaIII CATG 2 cut(s) 293, 596
NlaIV GGNNCC 4 cut(s) 129, 235, 236, 317
PceI AGGCCT 1 cut(s) 359
PflMI CCANNNNNTGG 1 cut(s) 244
PleI GAGTC 1 cut(s) 157
PmaCI CACGTG 1 cut(s) 241
PmlI CACGTG 1 cut(s) 241
PpsI GAGTC 1 cut(s) 157
Ppu21I YACGTR 1 cut(s) 241
PshAI GACNNNNGTC 1 cut(s) 284
Psp6I CCWGG 3 cut(s) 359, 486, 497
PspCI CACGTG 1 cut(s) 241
PspGI CCWGG 3 cut(s) 359, 486, 497
PspN4I GGNNCC 4 cut(s) 129, 235, 236, 317
PspOMI GGGCCC 1 cut(s) 234
PspPI GGNCC 4 cut(s) 234, 235, 286, 539
PsrI GAACNNNNNNTAC 2 cut(s) 589, 621
PstI CTGCAG 1 cut(s) 230
PsuI RGATCY 1 cut(s) 171
RsaI GTAC 4 cut(s) 146, 188, 464, 598
RsaNI GTAC 4 cut(s) 145, 187, 463, 597
Sau3AI GATC 2 cut(s) 171, 265
Sau96I GGNCC 4 cut(s) 234, 235, 286, 539
SchI GAGTC 1 cut(s) 158
ScrFI CCNGG 3 cut(s) 361, 488, 499
SduI GDGCHC 4 cut(s) 238, 318, 347, 448
SfaNI GCATC 1 cut(s) 637
SfcI CTRYAG 1 cut(s) 226
SinI GGWCC 2 cut(s) 286, 539
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 2 cut(s) 64, 504
SseBI AGGCCT 1 cut(s) 359
SspMI CTAG 2 cut(s) 48, 654
StuI AGGCCT 1 cut(s) 359
StyD4I CCNGG 3 cut(s) 359, 486, 497
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 2 cut(s) 62, 96
TaiI ACGT 1 cut(s) 243
TaqI TCGA 1 cut(s) 372
TasI AATT 2 cut(s) 64, 504
TauI GCSGC 8 cut(s) 17, 23, 26, 29, 35, 163, 211, 355
TscAI CASTG 2 cut(s) 42, 459
TspDTI ATGAA 1 cut(s) 627
TspGWI ACGGA 1 cut(s) 330
TspRI CASTG 2 cut(s) 42, 459
Van91I CCANNNNNTGG 1 cut(s) 244
VpaK11BI GGWCC 2 cut(s) 286, 539
XagI CCTNNNNNAGG 1 cut(s) 470
XspI CTAG 2 cut(s) 48, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.