Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0009776

Physical Location & Seq

Genomic Coordinates Forward (+)

8128376 .. 8135090

Gene Structure 6715 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g13560.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1815 bp

ATGAAGTACGTGTTGGTGACGGGTGGTGTTGTTAGTGGACTCGGCAAAGGAGTCACGGCCAGTAGTATTGGGTTGCTTCTTCAAGCGTGTGGCCTCCGTGTTACGTCCATCAAAATTGATCCTTACTTGAACACCGATGCTGGGACAATGTCTCCTTTTGAACATGGGGAGGTTTTTGTATTAGATGATGGCGGTGAGGTTGACCTTGACCTTGGAAATTATGAGCGGTTTCTGGATCTCACTTTGACCCGTGACAACAATATCACAACCGGAAAAATTTATCAGTCTGTTATTGACAAGGAGAGAAAGGGAGACTATCTTGGAAAGACTGTCCAGGTCGTTCCACACATTACGGATGCCATCCAAGAGTGGATAGAACGTGTGGCAATGGTACCTGTGGACGGGAAAGAAGGTCCAGCTGATGTATGTGTCATCGAATTGGGTGGAACTATAGGGGATATTGAATCGATGCCCTTTATTGAGGCCCTTGGCCAATTTTCGTATCGTGTTGGTCCTGGAAATTTCTGCTTGGTTCATGTCAGCCTTGTGCCTGTTCTGAATGTTGTTGGCGAGCAGAAAACAAAGCCTACACAGCACAGTGTTCGGGTACTCAGAGGACAGGGTTTGACACCTAATATTCTAGCTTGTAGGAGTACAAAGCCACTTGAGGAGAATGTCAAGGCAAAACTAGCTCAATTTTGTCACGTTCCGGCTGAAAATATTGTCACTCTGTATGATGTACCGAACATTTGGCACATCCCTTTGCTTTTAAGAGATCAAAAGGCACATGAAGCGATCTTGAAAGAACTAAACCTTCAAAGTATTGCTAGAGAGCCAAATTTGAAGGAATGGACAACCAGGACAGAAATTTCCTCAACTTTACATGATTCTGTCAGAATTGCAATGGTTGGAAAATACACTGGTCTTTCAGATGCTTACCTCTCTGTTCTGAAGGCTCTTTTACATGCTTCTGTTGCTTGCCATCGGAAACTTGTTGTAGATTGGGTTGCAGCAGGTGATCTTGAAGATGTTACTGCTAAAGAGACCCCTCAGGTTCATAAGGCTGCGTGGGATCTTTTGAAGGGCGCTGATGGTGTTCTAGTTCCAGGAGGATTTGGTGATAGAGGAGTGCAAGGGAAAATTCTTGCTGCAAAGTATGCTCGTGAAAACAAAGTTCCATTCCTTGGCATTTGCCTGGGAATGCAAATTGCTGTTATTGAGTTTGCAAGATCTGTCCTTGGTATGATTGATGCTAACAGCACAGAGTTTGATCCTGAAACTACAAGTCCTTGTGTCATATTTATGCCAGAGGGTTCCAAAACTCATATGGGAGGTACTATGCGTCTTGGATCAAGGAGGACCTACTTCAAGATTACTGACTGCAAATCTGCAAAACTGTACGGAGATGTGGCATTCATTGATGAGCGACATCGACATAGATATGAGGTCAATCCTGATATGATATCACAATTTGAAAGTGCTGGCTTATCATTTGTTGGCAGAGACGAAACCGGTCGGCGTATGGAGATTATTGAGCTGCCTGCCCATCCATACTTTGTTGGTGTTCAGTTCCATCCTGAATTTAAGTCGAGACCAGGCAAACCTTCTGCTCTGTTCTTAGGACTAATAACAGCATGCAGGCGTTCAGATACAAGCCTACCAAACAAAGGCCGTCTGAAAAAGCCTGTTGCAAATGGCACTAGTAATGGACACTTCACAGTGAAATCCCGCGAGAATGGACATCCATTTAAGTCATCAAATGGGTCACTAAATGGTGTGTATAGTAATGGTAATGGTAATGGTGTGCACTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003883 GO:0006139 GO:0006206 GO:0006213 GO:0006220 GO:0006221 GO:0006241 GO:0006629 GO:0006644 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009112 GO:0009116 GO:0009117 GO:0009119 GO:0009141 GO:0009142 GO:0009147 GO:0009148 GO:0009163 GO:0009165 GO:0009199 GO:0009201 GO:0009208 GO:0009209 GO:0009218 GO:0009220 GO:0009259 GO:0009260 GO:0009987 GO:0016874 GO:0016879 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019856 GO:0034404 GO:0034641 GO:0034654 GO:0042455 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0044281 GO:0044283 GO:0046036 GO:0046112 GO:0046131 GO:0046132 GO:0046134 GO:0046390 GO:0046483 GO:0055086 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

ko00240 ko01100 map00240 map01100

Pfam Domains

Protein Families

CTP_synth_N GATase

Protein Analysis

605

Amino Acids

65.96

Weight (kDa)

7.29

Isoelectric Point (pI)

37.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CTP_synth_N	PF06418	2 - 272	6.4e-123	CTP synthase N-terminus
GATase	PF00117	310 - 543	6.3e-42	Glutamine amidotransferase class-I

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009776)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12670
fragaria_vesca	FvH4_3g13560 FvH4_3g13560
malus_domestica	MD10G1220500.v1.1
prunus_persica	Prupe.4G123800_v2.0.a1 Prupe.4G123800_v2.0.a1 Prupe.4G123800_v2.0.a1
pyrus_communis	pycom05g21760 pycom10g18630
rosa_chinensis	RchiOBHm_Chr5g0022521
rosa_laevigata	RLG00000032668
rosa_multiflora	Rmu_sc0011329.1_g000002
rosa_roxburghii	Rroxscaffold_1G00056530
rosa_rugosa	Rorug05G0074500 Rorug05G0074600
rosa_samantha	Rh5AG161300 Rh5BG160200 Rh5CG174600 Rh5DG160900
rosa_wichuraiana	Rw0G005550 Rw5G014380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1004
Acc36I	ACCTGC	1 cut(s)	1004
Acc65I	GGTACC	1 cut(s)	391
AccB1I	GGYRCC	1 cut(s)	391
AccB7I	CCANNNNNTGG	1 cut(s)	1184
AccBSI	CCGCTC	1 cut(s)	226
AccII	CGCG	1 cut(s)	1731
AciI	CCGC	3 cut(s)	192, 226, 1729
AclWI	GGATC	5 cut(s)	113, 243, 1080, 1265, 1357
AcoI	YGGCCR	2 cut(s)	57, 490
AcsI	RAATTY	6 cut(s)	276, 520, 838, 867, 1140, 1580
AcuI	CTGAAG	1 cut(s)	971
AfaI	GTAC	7 cut(s)	8, 393, 609, 655, 741, 1336, 1400
AfiI	CCNNNNNNNGG	4 cut(s)	141, 401, 1184, 1667
AflIII	ACRYGT	2 cut(s)	9, 379
AgeI	ACCGGT	1 cut(s)	1511
AhlI	ACTAGT	1 cut(s)	1700
AjnI	CCWGG	6 cut(s)	333, 514, 857, 1105, 1194, 1594
AjuI	GAANNNNNNNTTGG	1 cut(s)	28
AluBI	AGCT	4 cut(s)	419, 644, 692, 1537
AluI	AGCT	4 cut(s)	419, 644, 692, 1537
Alw21I	GWGCWC	1 cut(s)	1809
Alw26I	GTCTC	5 cut(s)	156, 306, 1037, 1497, 1585
Alw44I	GTGCAC	1 cut(s)	1805
AlwI	GGATC	5 cut(s)	113, 243, 1080, 1265, 1357
AoxI	GGCC	5 cut(s)	57, 91, 483, 490, 1669
ApaLI	GTGCAC	1 cut(s)	1805
ApeKI	GCWGC	4 cut(s)	1010, 1064, 1148, 1537
ApoI	RAATTY	6 cut(s)	276, 520, 838, 867, 1140, 1580
AsiGI	ACCGGT	1 cut(s)	1511
Asp718I	GGTACC	1 cut(s)	391
AspLEI	GCGC	1 cut(s)	1088
AspS9I	GGNCC	4 cut(s)	413, 484, 512, 1359
AsuHPI	GGTGA	4 cut(s)	28, 206, 1028, 1130
AvaII	GGWCC	3 cut(s)	413, 512, 1359
AxyI	CCTNAGG	1 cut(s)	1050
BaeGI	GKGCMC	1 cut(s)	1809
BalI	TGGCCA	1 cut(s)	492
BanI	GGYRCC	1 cut(s)	391
BauI	CACGAG	1 cut(s)	1161
Bbv12I	GWGCWC	1 cut(s)	1809
BbvI	GCAGC	4 cut(s)	1022, 1051, 1135, 1524
BccI	CCATC	7 cut(s)	116, 182, 368, 990, 1085, 1554, 1581
BceAI	ACGGC	2 cut(s)	72, 1656
BcgI	CGANNNNNNTGC	2 cut(s)	584, 618
BciT130I	CCWGG	6 cut(s)	335, 516, 859, 1107, 1196, 1596
BcoDI	GTCTC	5 cut(s)	156, 306, 1037, 1497, 1585
BcuI	ACTAGT	1 cut(s)	1700
BfaI	CTAG	5 cut(s)	641, 689, 828, 1100, 1701
BfmI	CTRYAG	1 cut(s)	450
BfoI	RGCGCY	1 cut(s)	1089
BfuAI	ACCTGC	1 cut(s)	1004
BglII	AGATCT	1 cut(s)	1229
BisI	GCNGC	4 cut(s)	1011, 1065, 1149, 1538
BlsI	GCNGC	4 cut(s)	1012, 1066, 1150, 1539
Bme1390I	CCNGG	6 cut(s)	335, 516, 859, 1107, 1196, 1596
Bme18I	GGWCC	3 cut(s)	413, 512, 1359
BmgT120I	GGNCC	4 cut(s)	413, 484, 512, 1359
BmiI	GGNNCC	2 cut(s)	393, 1315
BmrFI	CCNGG	6 cut(s)	335, 516, 859, 1107, 1196, 1596
BmsI	GCATC	5 cut(s)	127, 346, 459, 922, 1240
BpuEI	CTTGAG	1 cut(s)	686
Bsa29I	ATCGAT	1 cut(s)	467
BsaAI	YACGTR	1 cut(s)	10
BsaI	GGTCTC	2 cut(s)	1037, 1585
BsaJI	CCNNGG	5 cut(s)	211, 487, 1183, 1195, 1237
BsaWI	WCCGGW	2 cut(s)	269, 1511
BsaXI	ACNNNNNCTCC	2 cut(s)	136, 166
Bsc4I	CCNNNNNNNGG	4 cut(s)	141, 401, 1184, 1667
Bse118I	RCCGGY	1 cut(s)	1511
Bse1I	ACTGG	2 cut(s)	60, 925
Bse21I	CCTNAGG	1 cut(s)	1050
Bse3DI	GCAATG	2 cut(s)	393, 909
BseBI	CCWGG	6 cut(s)	335, 516, 859, 1107, 1196, 1596
BseCI	ATCGAT	1 cut(s)	467
BseDI	CCNNGG	5 cut(s)	211, 487, 1183, 1195, 1237
BseGI	GGATG	6 cut(s)	360, 361, 756, 1546, 1573, 1741
BseLI	CCNNNNNNNGG	4 cut(s)	141, 401, 1184, 1667
BseMI	GCAATG	2 cut(s)	393, 909
BseMII	CTCAG	2 cut(s)	625, 1064
BseNI	ACTGG	2 cut(s)	60, 925
BseRI	GAGGAG	2 cut(s)	683, 1140
BseSI	GKGCMC	1 cut(s)	1809
BseXI	GCAGC	4 cut(s)	1022, 1051, 1135, 1524
BseYI	CCCAGC	1 cut(s)	140
Bsh1236I	CGCG	1 cut(s)	1731
Bsh1285I	CGRYCG	1 cut(s)	1516
BshFI	GGCC	5 cut(s)	59, 93, 485, 492, 1671
BshNI	GGYRCC	1 cut(s)	391
BshTI	ACCGGT	1 cut(s)	1511
BshVI	ATCGAT	1 cut(s)	467
BsiEI	CGRYCG	1 cut(s)	1516
BsiHKAI	GWGCWC	1 cut(s)	1809
BsiSI	CCGG	3 cut(s)	270, 710, 1512
BslFI	GGGAC	1 cut(s)	157
BslI	CCNNNNNNNGG	4 cut(s)	141, 401, 1184, 1667
BsmAI	GTCTC	5 cut(s)	156, 306, 1037, 1497, 1585
BsmBI	CGTCTC	1 cut(s)	1497
BsmFI	GGGAC	1 cut(s)	157
BsmI	GAATGC	2 cut(s)	1206, 1412
BsnI	GGCC	5 cut(s)	59, 93, 485, 492, 1671
Bso31I	GGTCTC	2 cut(s)	1037, 1585
Bsp1286I	GDGCHC	1 cut(s)	1809
Bsp143I	GATC	9 cut(s)	118, 235, 775, 795, 1018, 1072, 1229, 1270, 1349
BspACI	CCGC	3 cut(s)	192, 226, 1729
BspANI	GGCC	5 cut(s)	59, 93, 485, 492, 1671
BspCNI	CTCAG	2 cut(s)	624, 1063
BspDI	ATCGAT	1 cut(s)	467
BspFNI	CGCG	1 cut(s)	1731
BspLI	GGNNCC	2 cut(s)	393, 1315
BspMI	ACCTGC	1 cut(s)	1004
BspPI	GGATC	5 cut(s)	113, 243, 1080, 1265, 1357
BspT107I	GGYRCC	1 cut(s)	391
BspTNI	GGTCTC	2 cut(s)	1037, 1585
BsrBI	CCGCTC	1 cut(s)	226
BsrDI	GCAATG	2 cut(s)	393, 909
BsrFI	RCCGGY	1 cut(s)	1511
BsrI	ACTGG	2 cut(s)	60, 925
BssAI	RCCGGY	1 cut(s)	1511
BssECI	CCNNGG	5 cut(s)	211, 487, 1183, 1195, 1237
BssMI	GATC	9 cut(s)	118, 235, 775, 795, 1018, 1072, 1229, 1270, 1349
BssSI	CACGAG	1 cut(s)	1161
BssT1I	CCWWGG	4 cut(s)	211, 487, 1183, 1237
Bst2BI	CACGAG	1 cut(s)	1161
Bst2UI	CCWGG	6 cut(s)	335, 516, 859, 1107, 1196, 1596
Bst4CI	ACNGT	4 cut(s)	331, 599, 1398, 1720
BstAPI	GCANNNNNTGC	1 cut(s)	1157
BstBAI	YACGTR	1 cut(s)	10
BstC8I	GCNNGC	6 cut(s)	572, 979, 1483, 1542, 1636, 1640
BstDEI	CTNAG	3 cut(s)	611, 1050, 1618
BstF5I	GGATG	6 cut(s)	360, 361, 756, 1546, 1573, 1741
BstFNI	CGCG	1 cut(s)	1731
BstH2I	RGCGCY	1 cut(s)	1089
BstHHI	GCGC	1 cut(s)	1088
BstKTI	GATC	9 cut(s)	121, 238, 778, 798, 1021, 1075, 1232, 1273, 1352
BstMAI	GTCTC	5 cut(s)	156, 306, 1037, 1497, 1585
BstMBI	GATC	9 cut(s)	118, 235, 775, 795, 1018, 1072, 1229, 1270, 1349
BstMCI	CGRYCG	1 cut(s)	1516
BstMWI	GCNNNNNNNGC	5 cut(s)	592, 689, 791, 974, 1157
BstNI	CCWGG	6 cut(s)	335, 516, 859, 1107, 1196, 1596
BstNSI	RCATGY	2 cut(s)	968, 1638
BstSCI	CCNGG	6 cut(s)	333, 514, 857, 1105, 1194, 1594
BstSFI	CTRYAG	1 cut(s)	450
BstSLI	GKGCMC	1 cut(s)	1809
BstUI	CGCG	1 cut(s)	1731
BstV1I	GCAGC	4 cut(s)	1022, 1051, 1135, 1524
BstX2I	RGATCY	3 cut(s)	235, 1072, 1229
BstYI	RGATCY	3 cut(s)	235, 1072, 1229
Bsu15I	ATCGAT	1 cut(s)	467
Bsu36I	CCTNAGG	1 cut(s)	1050
BsuRI	GGCC	5 cut(s)	59, 93, 485, 492, 1671
BsuTUI	ATCGAT	1 cut(s)	467
BtsCI	GGATG	6 cut(s)	360, 361, 756, 1546, 1573, 1741
BtsIMutI	CAGTG	3 cut(s)	604, 918, 1725
BveI	ACCTGC	1 cut(s)	1004
Cac8I	GCNNGC	6 cut(s)	572, 979, 1483, 1542, 1636, 1640
CfoI	GCGC	1 cut(s)	1088
Cfr10I	RCCGGY	1 cut(s)	1511
Cfr13I	GGNCC	4 cut(s)	413, 484, 512, 1359
ClaI	ATCGAT	1 cut(s)	467
CseI	GACGC	1 cut(s)	1331
Csp6I	GTAC	7 cut(s)	7, 392, 608, 654, 740, 1335, 1399
CspAI	ACCGGT	1 cut(s)	1511
CviAII	CATG	6 cut(s)	164, 536, 788, 884, 965, 1635
CviQI	GTAC	7 cut(s)	7, 392, 608, 654, 740, 1335, 1399
DdeI	CTNAG	3 cut(s)	611, 1050, 1618
DpnI	GATC	9 cut(s)	120, 237, 777, 797, 1020, 1074, 1231, 1272, 1351
DpnII	GATC	9 cut(s)	118, 235, 775, 795, 1018, 1072, 1229, 1270, 1349
EaeI	YGGCCR	2 cut(s)	57, 490
Eco130I	CCWWGG	4 cut(s)	211, 487, 1183, 1237
Eco31I	GGTCTC	2 cut(s)	1037, 1585
Eco32I	GATATC	1 cut(s)	1464
Eco47I	GGWCC	3 cut(s)	413, 512, 1359
Eco57I	CTGAAG	1 cut(s)	971
Eco81I	CCTNAGG	1 cut(s)	1050
EcoO109I	RGGNCCY	2 cut(s)	484, 1359
EcoRII	CCWGG	6 cut(s)	333, 514, 857, 1105, 1194, 1594
EcoRV	GATATC	1 cut(s)	1464
EcoT14I	CCWWGG	4 cut(s)	211, 487, 1183, 1237
ErhI	CCWWGG	4 cut(s)	211, 487, 1183, 1237
Esp3I	CGTCTC	1 cut(s)	1497
FaeI	CATG	6 cut(s)	167, 539, 791, 887, 968, 1638
FaqI	GGGAC	1 cut(s)	157
FatI	CATG	6 cut(s)	163, 535, 787, 883, 964, 1634
FauI	CCCGC	1 cut(s)	1736
FauNDI	CATATG	1 cut(s)	1326
Fnu4HI	GCNGC	4 cut(s)	1011, 1065, 1149, 1538
FokI	GGATG	6 cut(s)	347, 368, 743, 1533, 1560, 1728
Fsp4HI	GCNGC	4 cut(s)	1011, 1065, 1149, 1538
FspBI	CTAG	5 cut(s)	641, 689, 828, 1100, 1701
GlaI	GCGC	1 cut(s)	1087
GluI	GCNGC	4 cut(s)	1011, 1065, 1149, 1538
GsaI	CCCAGC	1 cut(s)	144
HaeII	RGCGCY	1 cut(s)	1089
HaeIII	GGCC	5 cut(s)	59, 93, 485, 492, 1671
HapII	CCGG	3 cut(s)	270, 710, 1512
HgaI	GACGC	1 cut(s)	1331
HhaI	GCGC	1 cut(s)	1088
Hin1II	CATG	6 cut(s)	167, 539, 791, 887, 968, 1638
Hin6I	GCGC	1 cut(s)	1086
HinP1I	GCGC	1 cut(s)	1086
HincII	GTYRAC	1 cut(s)	202
HindII	GTYRAC	1 cut(s)	202
HinfI	GANTC	4 cut(s)	39, 51, 464, 887
HpaII	CCGG	3 cut(s)	270, 710, 1512
HphI	GGTGA	4 cut(s)	28, 206, 1028, 1130
Hpy166II	GTNNAC	4 cut(s)	38, 202, 400, 1807
Hpy188I	TCNGA	8 cut(s)	558, 614, 896, 931, 951, 987, 1648, 1677
Hpy188III	TCNNGA	9 cut(s)	233, 799, 1022, 1163, 1274, 1369, 1454, 1577, 1590
Hpy8I	GTNNAC	4 cut(s)	38, 202, 400, 1807
HpyAV	CCTTC	6 cut(s)	404, 824, 838, 946, 1075, 1614
HpyCH4III	ACNGT	4 cut(s)	331, 599, 1398, 1720
HpyCH4IV	ACGT	4 cut(s)	9, 104, 379, 705
HpyF10VI	GCNNNNNNNGC	5 cut(s)	592, 689, 791, 974, 1157
HpyF3I	CTNAG	3 cut(s)	611, 1050, 1618
HpySE526I	ACGT	4 cut(s)	9, 104, 379, 705
Hsp92II	CATG	6 cut(s)	167, 539, 791, 887, 968, 1638
HspAI	GCGC	1 cut(s)	1086
KpnI	GGTACC	1 cut(s)	395
Kzo9I	GATC	9 cut(s)	118, 235, 775, 795, 1018, 1072, 1229, 1270, 1349
Lsp1109I	GCAGC	4 cut(s)	1022, 1051, 1135, 1524
LweI	GCATC	5 cut(s)	127, 346, 459, 922, 1240
MaeI	CTAG	5 cut(s)	641, 689, 828, 1100, 1701
MaeII	ACGT	4 cut(s)	9, 104, 379, 705
MaeIII	GTNAC	8 cut(s)	16, 52, 100, 251, 701, 724, 1030, 1764
MalI	GATC	9 cut(s)	120, 237, 777, 797, 1020, 1074, 1231, 1272, 1351
MbiI	CCGCTC	1 cut(s)	226
MboI	GATC	9 cut(s)	118, 235, 775, 795, 1018, 1072, 1229, 1270, 1349
MboII	GAAGA	2 cut(s)	71, 1037
MflI	RGATCY	3 cut(s)	235, 1072, 1229
MhlI	GDGCHC	1 cut(s)	1809
MlsI	TGGCCA	1 cut(s)	492
MluNI	TGGCCA	1 cut(s)	492
MlyI	GAGTC	2 cut(s)	33, 60
MmeI	TCCRAC	1 cut(s)	889
Mox20I	TGGCCA	1 cut(s)	492
MscI	TGGCCA	1 cut(s)	492
MseI	TTAA	4 cut(s)	770, 1584, 1749, 1813
MslI	CAYNNNNRTG	2 cut(s)	1301, 1440
Msp20I	TGGCCA	1 cut(s)	492
MspA1I	CMGCKG	1 cut(s)	419
MspI	CCGG	3 cut(s)	270, 710, 1512
MspR9I	CCNGG	6 cut(s)	335, 516, 859, 1107, 1196, 1596
Mva1269I	GAATGC	2 cut(s)	1206, 1412
MvaI	CCWGG	6 cut(s)	335, 516, 859, 1107, 1196, 1596
MvnI	CGCG	1 cut(s)	1731
MwoI	GCNNNNNNNGC	5 cut(s)	592, 689, 791, 974, 1157
NdeI	CATATG	1 cut(s)	1326
NdeII	GATC	9 cut(s)	118, 235, 775, 795, 1018, 1072, 1229, 1270, 1349
NlaIII	CATG	6 cut(s)	167, 539, 791, 887, 968, 1638
NlaIV	GGNNCC	2 cut(s)	393, 1315
NmeAIII	GCCGAG	1 cut(s)	21
NmuCI	GTSAC	6 cut(s)	16, 52, 251, 701, 724, 1764
NspI	RCATGY	2 cut(s)	968, 1638
PaeI	GCATGC	1 cut(s)	1638
PaqCI	CACCTGC	1 cut(s)	1004
PctI	GAATGC	2 cut(s)	1206, 1412
PfeI	GAWTC	2 cut(s)	464, 887
PflFI	GACNNNGTC	1 cut(s)	148
PflMI	CCANNNNNTGG	1 cut(s)	1184
PfoI	TCCNGGA	2 cut(s)	514, 1105
PinAI	ACCGGT	1 cut(s)	1511
PkrI	GCNGC	4 cut(s)	1012, 1066, 1150, 1539
PleI	GAGTC	2 cut(s)	33, 59
PpsI	GAGTC	2 cut(s)	33, 59
Ppu21I	YACGTR	1 cut(s)	10
PpuMI	RGGWCCY	1 cut(s)	1359
Psp5II	RGGWCCY	1 cut(s)	1359
Psp6I	CCWGG	6 cut(s)	333, 514, 857, 1105, 1194, 1594
PspFI	CCCAGC	1 cut(s)	140
PspGI	CCWGG	6 cut(s)	333, 514, 857, 1105, 1194, 1594
PspN4I	GGNNCC	2 cut(s)	393, 1315
PspPI	GGNCC	4 cut(s)	413, 484, 512, 1359
PspPPI	RGGWCCY	1 cut(s)	1359
PsuI	RGATCY	3 cut(s)	235, 1072, 1229
PsyI	GACNNNGTC	1 cut(s)	148
PvuII	CAGCTG	1 cut(s)	419
RsaI	GTAC	7 cut(s)	8, 393, 609, 655, 741, 1336, 1400
RsaNI	GTAC	7 cut(s)	7, 392, 608, 654, 740, 1335, 1399
RseI	CAYNNNNRTG	2 cut(s)	1301, 1440
SaqAI	TTAA	4 cut(s)	770, 1584, 1749, 1813
SatI	GCNGC	4 cut(s)	1011, 1065, 1149, 1538
Sau3AI	GATC	9 cut(s)	118, 235, 775, 795, 1018, 1072, 1229, 1270, 1349
Sau96I	GGNCC	4 cut(s)	413, 484, 512, 1359
SchI	GAGTC	2 cut(s)	33, 60
ScrFI	CCNGG	6 cut(s)	335, 516, 859, 1107, 1196, 1596
SduI	GDGCHC	1 cut(s)	1809
SfaNI	GCATC	5 cut(s)	127, 346, 459, 922, 1240
SfcI	CTRYAG	1 cut(s)	450
SinI	GGWCC	3 cut(s)	413, 512, 1359
SmiMI	CAYNNNNRTG	2 cut(s)	1301, 1440
SmlI	CTYRAG	1 cut(s)	665
SmoI	CTYRAG	1 cut(s)	665
SpeI	ACTAGT	1 cut(s)	1700
SphI	GCATGC	1 cut(s)	1638
SsiI	CCGC	3 cut(s)	192, 226, 1729
SspI	AATATT	2 cut(s)	637, 721
SspMI	CTAG	5 cut(s)	641, 689, 828, 1100, 1701
StyD4I	CCNGG	6 cut(s)	333, 514, 857, 1105, 1194, 1594
StyI	CCWWGG	4 cut(s)	211, 487, 1183, 1237
TaaI	ACNGT	4 cut(s)	331, 599, 1398, 1720
TaiI	ACGT	4 cut(s)	12, 107, 382, 708
TaqI	TCGA	4 cut(s)	435, 467, 1432, 1589
TatI	WGTACW	1 cut(s)	653
TfiI	GAWTC	2 cut(s)	464, 887
Tru1I	TTAA	4 cut(s)	770, 1584, 1749, 1813
Tru9I	TTAA	4 cut(s)	770, 1584, 1749, 1813
TscAI	CASTG	3 cut(s)	604, 925, 1725
TseFI	GTSAC	6 cut(s)	16, 52, 251, 701, 724, 1764
TseI	GCWGC	4 cut(s)	1010, 1064, 1148, 1537
Tsp45I	GTSAC	6 cut(s)	16, 52, 251, 701, 724, 1764
TspDTI	ATGAA	5 cut(s)	17, 524, 804, 1046, 1405
TspGWI	ACGGA	3 cut(s)	86, 368, 1416
TspRI	CASTG	3 cut(s)	604, 925, 1725
Tth111I	GACNNNGTC	1 cut(s)	148
Van91I	CCANNNNNTGG	1 cut(s)	1184
VneI	GTGCAC	1 cut(s)	1805
VpaK11BI	GGWCC	3 cut(s)	413, 512, 1359
XapI	RAATTY	6 cut(s)	276, 520, 838, 867, 1140, 1580
XceI	RCATGY	2 cut(s)	968, 1638
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1324
XspI	CTAG	5 cut(s)	641, 689, 828, 1100, 1701

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.