FvH4_3g21240

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015574
Physical Location & Seq
Reverse (-)
14229731 .. 14231685
1955 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21240.t1

Sequence Viewer

Length: 426 bp
ATGGCCACAGTACTAGATCACCATCACATGAATAATGCAGCAGTTGAGTTCGTGGACTACTTCCCGTCCATGATCCTGCGTCTCGGAGAAGAAGCGTTCCTCGCCGAGCTGTGCAGCGGGTTTCGGATGCTGATGGACGCGGAGAAAGGGCTGATCACGTTCGAAAGCTTGAAGCGGAACAGCCTGTTGCTGGGGATTCATGACATGGGGGACGATGAGCTTGTGTGGATGTTGATGGAAGGTGATCTCGATGGAGACGGGGCTCTCAGCCAGTTGGAGTTTTGCATTCTTATGTTTAGGTTAAGTCCAGGTCTGATGGATGCAGGACCAAATAAGCAGGTTTGGATGGAGAATAATGTTGATCGTCCTCATCATCATGTAAATGATCAGATACATGAAGCCTCTCTGGTCGTGCAGACGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

142

Amino Acids

15.95

Weight (kDa)

4.6

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 48 - 100 2e-15 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015574)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21240
malus_domestica MD03G1186200.v1.1 MD11G1233900.v1.1
prunus_persica Prupe.4G192800_v2.0.a1
pyrus_communis pycom11g20610
rosa_chinensis RchiOBHm_Chr5g0036321
rosa_laevigata RLG00000033686
rosa_multiflora Rmu_sc0000247.1_g000078
rosa_roxburghii Rroxscaffold_1G00044470
rosa_rugosa Rorug05G0155100
rosa_samantha Rh5AG248400 Rh5BG249200 Rh5CG280500 Rh5DG257700
rosa_wichuraiana Rw0G002060 Rw5G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 328
AccII CGCG 1 cut(s) 140
AciI CCGC 3 cut(s) 117, 140, 175
AclWI GGATC 1 cut(s) 67
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 1 cut(s) 190
AgsI TTSAA 1 cut(s) 172
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 3 cut(s) 109, 168, 220
AluI AGCT 3 cut(s) 109, 168, 220
Alw26I GTCTC 2 cut(s) 86, 249
AlwI GGATC 1 cut(s) 67
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 38, 114
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 2 cut(s) 11, 254
AsuII TTCGAA 1 cut(s) 162
AvaII GGWCC 1 cut(s) 326
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 265
BbvI GCAGC 2 cut(s) 50, 126
BccI CCATC 6 cut(s) 30, 127, 229, 245, 310, 340
BciT130I CCWGG 1 cut(s) 309
BclI TGATCA 2 cut(s) 153, 385
BcoDI GTCTC 2 cut(s) 86, 249
BfaI CTAG 1 cut(s) 14
BfuAI ACCTGC 1 cut(s) 328
BisI GCNGC 2 cut(s) 39, 115
BlsI GCNGC 2 cut(s) 40, 116
BmcAI AGTACT 1 cut(s) 12
Bme1390I CCNGG 1 cut(s) 309
Bme18I GGWCC 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 326
BmrFI CCNGG 1 cut(s) 309
BmsI GCATC 2 cut(s) 117, 310
Bpu14I TTCGAA 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 21
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 271
Bse8I GATNNNNATC 1 cut(s) 21
BseBI CCWGG 1 cut(s) 309
BseGI GGATG 4 cut(s) 132, 234, 325, 351
BseJI GATNNNNATC 1 cut(s) 21
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 280
BseNI ACTGG 1 cut(s) 271
BseXI GCAGC 2 cut(s) 50, 126
BseYI CCCAGC 1 cut(s) 190
BsgI GTGCAG 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 1 cut(s) 5
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 1 cut(s) 190
BsmAI GTCTC 2 cut(s) 86, 249
BsmBI CGTCTC 2 cut(s) 86, 249
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 285
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 265
Bsp143I GATC 6 cut(s) 16, 72, 153, 244, 361, 385
BspACI CCGC 3 cut(s) 117, 140, 175
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 279
BspFNI CGCG 1 cut(s) 140
BspHI TCATGA 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 328
BspPI GGATC 1 cut(s) 67
BspT104I TTCGAA 1 cut(s) 162
BsrI ACTGG 1 cut(s) 271
BssMI GATC 6 cut(s) 16, 72, 153, 244, 361, 385
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 10
BstBI TTCGAA 1 cut(s) 162
BstDEI CTNAG 1 cut(s) 266
BstF5I GGATG 4 cut(s) 132, 234, 325, 351
BstFNI CGCG 1 cut(s) 140
BstKTI GATC 6 cut(s) 19, 75, 156, 247, 364, 388
BstMAI GTCTC 2 cut(s) 86, 249
BstMBI GATC 6 cut(s) 16, 72, 153, 244, 361, 385
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 307
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 2 cut(s) 50, 126
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 4 cut(s) 132, 234, 325, 351
BveI ACCTGC 1 cut(s) 328
CciI TCATGA 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 326
CseI GACGC 2 cut(s) 68, 146
Csp6I GTAC 1 cut(s) 11
CviAII CATG 6 cut(s) 28, 70, 200, 205, 377, 395
CviJI RGCY 9 cut(s) 5, 109, 151, 168, 183, 220, 263, 270, 401
CviKI_1 RGCY 9 cut(s) 5, 109, 151, 168, 183, 220, 263, 270, 401
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 1 cut(s) 266
DpnI GATC 6 cut(s) 18, 74, 155, 246, 363, 387
DpnII GATC 6 cut(s) 16, 72, 153, 244, 361, 385
EaeI YGGCCR 1 cut(s) 3
Eco24I GRGCYC 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 307
EcoT38I GRGCYC 1 cut(s) 265
Esp3I CGTCTC 2 cut(s) 86, 249
FaeI CATG 6 cut(s) 31, 73, 203, 208, 380, 398
FaiI YATR 7 cut(s) 29, 71, 201, 206, 293, 378, 396
FaqI GGGAC 1 cut(s) 224
FatI CATG 6 cut(s) 27, 69, 199, 204, 376, 394
FauI CCCGC 1 cut(s) 110
FbaI TGATCA 2 cut(s) 153, 385
Fnu4HI GCNGC 2 cut(s) 39, 115
FokI GGATG 4 cut(s) 139, 241, 332, 358
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 2 cut(s) 39, 115
FspBI CTAG 1 cut(s) 14
GluI GCNGC 2 cut(s) 39, 115
GsaI CCCAGC 1 cut(s) 194
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 2 cut(s) 68, 146
Hin1II CATG 6 cut(s) 31, 73, 203, 208, 380, 398
HindIII AAGCTT 1 cut(s) 166
HinfI GANTC 1 cut(s) 196
HphI GGTGA 2 cut(s) 11, 254
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 4 cut(s) 86, 126, 315, 390
Hpy188III TCNNGA 2 cut(s) 200, 248
Hpy8I GTNNAC 1 cut(s) 55
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 5 cut(s) 38, 114, 285, 323, 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
HpyF3I CTNAG 1 cut(s) 266
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 6 cut(s) 31, 73, 203, 208, 380, 398
Ksp22I TGATCA 2 cut(s) 153, 385
Kzo9I GATC 6 cut(s) 16, 72, 153, 244, 361, 385
LpnPI CCDG 9 cut(s) 89, 176, 197, 284, 294, 309, 321, 323, 392
Lsp1109I GCAGC 2 cut(s) 50, 126
LweI GCATC 2 cut(s) 117, 310
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 158
MalI GATC 6 cut(s) 18, 74, 155, 246, 363, 387
MboI GATC 6 cut(s) 16, 72, 153, 244, 361, 385
MboII GAAGA 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 265
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 3 cut(s) 110, 378, 412
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 302
MslI CAYNNNNRTG 3 cut(s) 290, 375, 381
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 285
MvaI CCWGG 1 cut(s) 309
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 1 cut(s) 101
NdeII GATC 6 cut(s) 16, 72, 153, 244, 361, 385
NlaIII CATG 6 cut(s) 31, 73, 203, 208, 380, 398
NmeAIII GCCGAG 1 cut(s) 130
NspV TTCGAA 1 cut(s) 162
PagI TCATGA 1 cut(s) 199
PctI GAATGC 1 cut(s) 285
PfeI GAWTC 1 cut(s) 196
PkrI GCNGC 2 cut(s) 40, 116
Psp6I CCWGG 1 cut(s) 307
PspFI CCCAGC 1 cut(s) 190
PspGI CCWGG 1 cut(s) 307
PspPI GGNCC 1 cut(s) 326
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
RseI CAYNNNNRTG 3 cut(s) 290, 375, 381
SaqAI TTAA 1 cut(s) 302
SatI GCNGC 2 cut(s) 39, 115
Sau3AI GATC 6 cut(s) 16, 72, 153, 244, 361, 385
Sau96I GGNCC 1 cut(s) 326
ScaI AGTACT 1 cut(s) 12
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 1 cut(s) 265
SetI ASST 8 cut(s) 111, 161, 170, 222, 244, 302, 313, 342
SfaNI GCATC 2 cut(s) 117, 310
SfuI TTCGAA 1 cut(s) 162
SinI GGWCC 1 cut(s) 326
SmiMI CAYNNNNRTG 3 cut(s) 290, 375, 381
SsiI CCGC 3 cut(s) 117, 140, 175
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 1 cut(s) 307
TaaI ACNGT 1 cut(s) 10
TaiI ACGT 1 cut(s) 161
TaqI TCGA 2 cut(s) 162, 249
TatI WGTACW 1 cut(s) 10
TfiI GAWTC 1 cut(s) 196
Tru1I TTAA 1 cut(s) 302
Tru9I TTAA 1 cut(s) 302
TseI GCWGC 2 cut(s) 38, 114
TspDTI ATGAA 3 cut(s) 44, 188, 411
VpaK11BI GGWCC 1 cut(s) 326
XspI CTAG 1 cut(s) 14
ZrmI AGTACT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.