Rw5G022810

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015574
Physical Location & Seq
Reverse (-)
31789323 .. 31791304
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022810.1

Sequence Viewer

Length: 399 bp
ATGGCCACAGTACTAGAGCATCACATCAATAACGCCGCAGTTGAGTTCGTGGACTACTTTCCTTCCATGATTCTGCGGCTCGGAGAAGAAGCGTTCATCGCAGAGCTGTGCAGCGGGTTTCGGCTGCTGATGGATGTGGAGAAAGGGCTCATCACGTTCGAAAGCTTGAAGCGGAACAGCCTCTTGCTGGGGATTCATGACATGGGGGACGATGAGCTTGTGTGGATGTTGATGGAAGGTGATCTCGATGGAGACGGGGCTCTCAGCCAGTTGGAGTTTTGCATTCTCATGTTTAGGTTAAGTCCAGGTCTGATGGATGCAGGACCTAATAAGCAGGTTTGGATGGAGGACATTGATCGTCGTCGTCGTCATGTAAATGATCAGATACATGTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

132

Amino Acids

15.13

Weight (kDa)

4.61

Isoelectric Point (pI)

53.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 47 - 99 1.8e-15 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015574)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21240
malus_domestica MD03G1186200.v1.1 MD11G1233900.v1.1
prunus_persica Prupe.4G192800_v2.0.a1
pyrus_communis pycom11g20610
rosa_chinensis RchiOBHm_Chr5g0036321
rosa_laevigata RLG00000033686
rosa_multiflora Rmu_sc0000247.1_g000078
rosa_roxburghii Rroxscaffold_1G00044470
rosa_rugosa Rorug05G0155100
rosa_samantha Rh5AG248400 Rh5BG249200 Rh5CG280500 Rh5DG257700
rosa_wichuraiana Rw0G002060 Rw5G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 325
AciI CCGC 4 cut(s) 36, 76, 114, 172
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 1 cut(s) 187
AflIII ACRYGT 1 cut(s) 388
AgsI TTSAA 1 cut(s) 169
AjnI CCWGG 1 cut(s) 304
AluBI AGCT 3 cut(s) 106, 165, 217
AluI AGCT 3 cut(s) 106, 165, 217
Alw26I GTCTC 1 cut(s) 246
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 111, 124
AspS9I GGNCC 1 cut(s) 323
AsuHPI GGTGA 1 cut(s) 251
AsuII TTCGAA 1 cut(s) 159
AvaII GGWCC 1 cut(s) 323
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 2 cut(s) 150, 262
BbvI GCAGC 2 cut(s) 111, 123
BccI CCATC 5 cut(s) 124, 226, 242, 307, 337
BciT130I CCWGG 1 cut(s) 306
BclI TGATCA 1 cut(s) 379
BcoDI GTCTC 1 cut(s) 246
BfaI CTAG 2 cut(s) 14, 397
BfuAI ACCTGC 1 cut(s) 325
BisI GCNGC 4 cut(s) 36, 77, 112, 125
BlsI GCNGC 4 cut(s) 37, 78, 113, 126
BmcAI AGTACT 1 cut(s) 12
Bme1390I CCNGG 1 cut(s) 306
Bme18I GGWCC 1 cut(s) 323
BmgT120I GGNCC 1 cut(s) 323
BmrFI CCNGG 1 cut(s) 306
BmsI GCATC 2 cut(s) 28, 307
Bpu14I TTCGAA 1 cut(s) 159
Bsc4I CCNNNNNNNGG 1 cut(s) 187
Bse1I ACTGG 1 cut(s) 268
BseBI CCWGG 1 cut(s) 306
BseGI GGATG 4 cut(s) 139, 231, 322, 348
BseLI CCNNNNNNNGG 1 cut(s) 187
BseMII CTCAG 1 cut(s) 277
BseNI ACTGG 1 cut(s) 268
BseXI GCAGC 2 cut(s) 111, 123
BseYI CCCAGC 1 cut(s) 187
BsgI GTGCAG 1 cut(s) 130
BshFI GGCC 1 cut(s) 5
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 1 cut(s) 187
BsmAI GTCTC 1 cut(s) 246
BsmBI CGTCTC 1 cut(s) 246
BsmFI GGGAC 1 cut(s) 221
BsmI GAATGC 1 cut(s) 282
BsnI GGCC 1 cut(s) 5
Bsp119I TTCGAA 1 cut(s) 159
Bsp1286I GDGCHC 2 cut(s) 150, 262
Bsp143I GATC 3 cut(s) 241, 355, 379
BspACI CCGC 4 cut(s) 36, 76, 114, 172
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 276
BspHI TCATGA 1 cut(s) 196
BspMI ACCTGC 1 cut(s) 325
BspT104I TTCGAA 1 cut(s) 159
BsrI ACTGG 1 cut(s) 268
BssMI GATC 3 cut(s) 241, 355, 379
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 1 cut(s) 10
BstBI TTCGAA 1 cut(s) 159
BstDEI CTNAG 1 cut(s) 263
BstF5I GGATG 4 cut(s) 139, 231, 322, 348
BstKTI GATC 3 cut(s) 244, 358, 382
BstMAI GTCTC 1 cut(s) 246
BstMBI GATC 3 cut(s) 241, 355, 379
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 1 cut(s) 392
BstSCI CCNGG 1 cut(s) 304
BstV1I GCAGC 2 cut(s) 111, 123
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 82
BtsCI GGATG 4 cut(s) 139, 231, 322, 348
BveI ACCTGC 1 cut(s) 325
CciI TCATGA 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 323
Csp6I GTAC 1 cut(s) 11
CviAII CATG 6 cut(s) 67, 197, 202, 289, 371, 389
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 1 cut(s) 263
DpnI GATC 3 cut(s) 243, 357, 381
DpnII GATC 3 cut(s) 241, 355, 379
EaeI YGGCCR 1 cut(s) 3
Eco24I GRGCYC 2 cut(s) 150, 262
Eco47I GGWCC 1 cut(s) 323
EcoO109I RGGNCCY 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 304
EcoT38I GRGCYC 2 cut(s) 150, 262
Esp3I CGTCTC 1 cut(s) 246
FaeI CATG 6 cut(s) 70, 200, 205, 292, 374, 392
FaiI YATR 6 cut(s) 68, 198, 203, 290, 372, 390
FaqI GGGAC 1 cut(s) 221
FatI CATG 6 cut(s) 66, 196, 201, 288, 370, 388
FauI CCCGC 1 cut(s) 107
FbaI TGATCA 1 cut(s) 379
Fnu4HI GCNGC 4 cut(s) 36, 77, 112, 125
FokI GGATG 4 cut(s) 146, 238, 329, 355
FriOI GRGCYC 2 cut(s) 150, 262
Fsp4HI GCNGC 4 cut(s) 36, 77, 112, 125
FspBI CTAG 2 cut(s) 14, 397
GluI GCNGC 4 cut(s) 36, 77, 112, 125
GsaI CCCAGC 1 cut(s) 191
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 6 cut(s) 70, 200, 205, 292, 374, 392
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 2 cut(s) 70, 193
HphI GGTGA 1 cut(s) 251
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 3 cut(s) 83, 312, 384
Hpy188III TCNNGA 2 cut(s) 197, 245
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 3 cut(s) 363, 366, 369
HpyAV CCTTC 2 cut(s) 72, 230
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 3 cut(s) 111, 282, 320
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 1 cut(s) 263
HpySE526I ACGT 1 cut(s) 155
Hsp92II CATG 6 cut(s) 70, 200, 205, 292, 374, 392
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 3 cut(s) 241, 355, 379
LpnPI CCDG 6 cut(s) 173, 281, 291, 306, 318, 320
Lsp1109I GCAGC 2 cut(s) 111, 123
LweI GCATC 2 cut(s) 28, 307
MaeI CTAG 2 cut(s) 14, 397
MaeII ACGT 1 cut(s) 155
MalI GATC 3 cut(s) 243, 357, 381
MboI GATC 3 cut(s) 241, 355, 379
MboII GAAGA 1 cut(s) 98
MhlI GDGCHC 2 cut(s) 150, 262
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 252
MnlI CCTC 2 cut(s) 191, 340
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 299
MslI CAYNNNNRTG 2 cut(s) 287, 375
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 114
MspR9I CCNGG 1 cut(s) 306
Mva1269I GAATGC 1 cut(s) 282
MvaI CCWGG 1 cut(s) 306
MwoI GCNNNNNNNGC 1 cut(s) 98
NdeII GATC 3 cut(s) 241, 355, 379
NlaIII CATG 6 cut(s) 70, 200, 205, 292, 374, 392
NspI RCATGY 1 cut(s) 392
NspV TTCGAA 1 cut(s) 159
PagI TCATGA 1 cut(s) 196
PciI ACATGT 1 cut(s) 388
PcsI WCGNNNNNNNCGW 1 cut(s) 364
PctI GAATGC 1 cut(s) 282
PfeI GAWTC 2 cut(s) 70, 193
PkrI GCNGC 4 cut(s) 37, 78, 113, 126
PpuMI RGGWCCY 1 cut(s) 323
PscI ACATGT 1 cut(s) 388
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 304
PspFI CCCAGC 1 cut(s) 187
PspGI CCWGG 1 cut(s) 304
PspPI GGNCC 1 cut(s) 323
PspPPI RGGWCCY 1 cut(s) 323
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
RseI CAYNNNNRTG 2 cut(s) 287, 375
SaqAI TTAA 1 cut(s) 299
SatI GCNGC 4 cut(s) 36, 77, 112, 125
Sau3AI GATC 3 cut(s) 241, 355, 379
Sau96I GGNCC 1 cut(s) 323
ScaI AGTACT 1 cut(s) 12
ScrFI CCNGG 1 cut(s) 306
SduI GDGCHC 2 cut(s) 150, 262
SetI ASST 9 cut(s) 108, 158, 167, 219, 241, 299, 310, 328, 339
SfaNI GCATC 2 cut(s) 28, 307
SfuI TTCGAA 1 cut(s) 159
SinI GGWCC 1 cut(s) 323
SmiMI CAYNNNNRTG 2 cut(s) 287, 375
SsiI CCGC 4 cut(s) 36, 76, 114, 172
SspMI CTAG 2 cut(s) 14, 397
StyD4I CCNGG 1 cut(s) 304
TaaI ACNGT 1 cut(s) 10
TaiI ACGT 1 cut(s) 158
TaqI TCGA 2 cut(s) 159, 246
TatI WGTACW 1 cut(s) 10
TauI GCSGC 2 cut(s) 38, 79
TfiI GAWTC 2 cut(s) 70, 193
Tru1I TTAA 1 cut(s) 299
Tru9I TTAA 1 cut(s) 299
TseI GCWGC 2 cut(s) 111, 124
TspDTI ATGAA 2 cut(s) 85, 185
VpaK11BI GGWCC 1 cut(s) 323
XceI RCATGY 1 cut(s) 392
XspI CTAG 2 cut(s) 14, 397
ZrmI AGTACT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.