Rorug05G0155100

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0015574
Physical Location & Seq
Forward (+)
14434917 .. 14440008
5092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0155100.1

Sequence Viewer

Length: 903 bp
ATGCGGGAGATGTTGAAGATTCAGGACACAAGGATCTACATCTGGCAACTGCACCAAGAAGATCAAATGGTGGAGCCATCAGAGGGTCCAGTCCAAGCTGATGATCATGCTGGAAGCAATAGTTTGAAATCAAGGGAGTCCTCCGATGCTAAAGAAGAAACCTCGAGACAAAAGATTGTTATTAAAGAGGAAAAGGCAGCTGTTGCATCTAGGTATATGCAAGGTTTTTCGACATTAAATTCAAAAGCTAACTCACAAGATTCTAGTGGAGGTGGGAAGCACAATGATAATGAGAGTGGTGGTAAGAAGCAGGTTGGCATGATAAAAGGCAAACAGCAAGAGCTTAATGGTCAGGTACGCTCGATAACTCCTTCCCACAGCAGAGCTGGTGCACTTTCATTAAAGCCAGAAGCAGCTGTATCGAACAATCCCTCCTTAAAATTTAAATTGTATAGCCTCCTCATTGTCAAGGTGGAAGAATCAAATTGGAAGAGCAGAAAGATGGAAATGCAGCAATACTGTGCACGTGAATGGAAGTCCATCTTAGGTGCAACAGCCGATTCTGAAAATTCTAAAGGCGGCTTTGACTGCAACATCTGCTCAGACTTTTCACACGAGCCAGTGGTCACCCTCTGTGGCCATCTGTACTGCTGGCCTTGCATCTACAAAGGGCTTCACGTCCAGAGCGCCTCCCTTGCGTTTGATGAGTGCCCTCAGTGCCCTGTTTGCAAGAAACAAGATCATTTGTTTGTATGCTGCTTAACTTTTTATACAAGTGAAGATCCTCAGGTTTCACACATTAGGAAACTGACCGTGTTACATATTGAAGTTGATCCCATATTAGCCTCCTTCTCTGGAGGAGCTGTGGGAGTGATCTTAACACTGACGGTGGCTGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

300

Amino Acids

33.2

Weight (kDa)

6.79

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_UBOX PF13445 197 - 241 2.5e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015574)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21240
malus_domestica MD03G1186200.v1.1 MD11G1233900.v1.1
prunus_persica Prupe.4G192800_v2.0.a1
pyrus_communis pycom11g20610
rosa_chinensis RchiOBHm_Chr5g0036321
rosa_laevigata RLG00000033686
rosa_multiflora Rmu_sc0000247.1_g000078
rosa_roxburghii Rroxscaffold_1G00044470
rosa_rugosa Rorug05G0155100
rosa_samantha Rh5AG248400 Rh5BG249200 Rh5CG280500 Rh5DG257700
rosa_wichuraiana Rw0G002060 Rw5G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 301
AciI CCGC 2 cut(s) 4, 579
AclWI GGATC 3 cut(s) 41, 776, 827
AcoI YGGCCR 1 cut(s) 637
AcsI RAATTY 3 cut(s) 238, 440, 568
AcvI CACGTG 1 cut(s) 527
AfaI GTAC 2 cut(s) 357, 647
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 4 cut(s) 16, 127, 243, 827
AjiI CACGTC 1 cut(s) 679
AloI GAACNNNNNNTCC 2 cut(s) 416, 448
AluBI AGCT 7 cut(s) 98, 200, 248, 343, 386, 416, 863
AluI AGCT 7 cut(s) 98, 200, 248, 343, 386, 416, 863
Alw21I GWGCWC 2 cut(s) 394, 526
Alw26I GTCTC 1 cut(s) 160
Alw44I GTGCAC 2 cut(s) 390, 522
AlwI GGATC 3 cut(s) 41, 776, 827
Ama87I CYCGRG 1 cut(s) 163
AoxI GGCC 2 cut(s) 637, 653
ApaLI GTGCAC 2 cut(s) 390, 522
ApeKI GCWGC 4 cut(s) 197, 413, 511, 756
ApoI RAATTY 3 cut(s) 238, 440, 568
AspLEI GCGC 1 cut(s) 689
AspS9I GGNCC 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 619
AvaI CYCGRG 1 cut(s) 163
AvaII GGWCC 1 cut(s) 86
AxyI CCTNAGG 1 cut(s) 786
BaeGI GKGCMC 4 cut(s) 394, 526, 713, 722
BalI TGGCCA 1 cut(s) 639
BauI CACGAG 1 cut(s) 614
BbrPI CACGTG 1 cut(s) 527
Bbv12I GWGCWC 2 cut(s) 394, 526
BbvI GCAGC 4 cut(s) 209, 425, 523, 743
BccI CCATC 4 cut(s) 85, 496, 548, 648
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BclI TGATCA 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 2 cut(s) 210, 264
BfoI RGCGCY 1 cut(s) 690
BfuAI ACCTGC 1 cut(s) 301
BisI GCNGC 5 cut(s) 198, 414, 512, 580, 757
BlsI GCNGC 5 cut(s) 199, 415, 513, 581, 758
Bme18I GGWCC 1 cut(s) 86
BmeT110I CYCGRG 1 cut(s) 163
BmgBI CACGTC 1 cut(s) 679
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 2 cut(s) 75, 87
BmsI GCATC 3 cut(s) 136, 215, 669
BpmI CTGGAG 1 cut(s) 876
BsaAI YACGTR 1 cut(s) 527
BsaBI GATNNNNATC 1 cut(s) 38
BsaXI ACNNNNNCTCC 4 cut(s) 416, 446, 849, 879
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse1I ACTGG 2 cut(s) 89, 620
Bse21I CCTNAGG 1 cut(s) 786
Bse8I GATNNNNATC 1 cut(s) 38
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 4 cut(s) 615, 728, 800, 885
BseNI ACTGG 2 cut(s) 89, 620
BseRI GAGGAG 2 cut(s) 449, 873
BseSI GKGCMC 4 cut(s) 394, 526, 713, 722
BseXI GCAGC 4 cut(s) 209, 425, 523, 743
BsgI GTGCAG 1 cut(s) 35
BshFI GGCC 2 cut(s) 639, 655
BsiHKAI GWGCWC 2 cut(s) 394, 526
BsiHKCI CYCGRG 1 cut(s) 163
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 160
BsnI GGCC 2 cut(s) 639, 655
BsoBI CYCGRG 1 cut(s) 163
Bsp1286I GDGCHC 4 cut(s) 394, 526, 713, 722
Bsp143I GATC 7 cut(s) 33, 61, 103, 739, 781, 832, 873
BspACI CCGC 2 cut(s) 4, 579
BspANI GGCC 2 cut(s) 639, 655
BspCNI CTCAG 4 cut(s) 614, 727, 799, 886
BspLI GGNNCC 2 cut(s) 75, 87
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 3 cut(s) 41, 776, 827
BspQI GCTCTTC 1 cut(s) 485
BsrI ACTGG 2 cut(s) 89, 620
BssMI GATC 7 cut(s) 33, 61, 103, 739, 781, 832, 873
BssSI CACGAG 1 cut(s) 614
Bst2BI CACGAG 1 cut(s) 614
Bst4CI ACNGT 3 cut(s) 521, 814, 889
Bst6I CTCTTC 1 cut(s) 485
BstAPI GCANNNNNTGC 2 cut(s) 203, 597
BstBAI YACGTR 1 cut(s) 527
BstC8I GCNNGC 1 cut(s) 653
BstDEI CTNAG 5 cut(s) 544, 601, 714, 786, 894
BstEII GGTNACC 1 cut(s) 625
BstH2I RGCGCY 1 cut(s) 690
BstHHI GCGC 1 cut(s) 689
BstKTI GATC 7 cut(s) 36, 64, 106, 742, 784, 835, 876
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 7 cut(s) 33, 61, 103, 739, 781, 832, 873
BstMWI GCNNNNNNNGC 7 cut(s) 203, 588, 597, 657, 695, 717, 726
BstPI GGTNACC 1 cut(s) 625
BstSLI GKGCMC 4 cut(s) 394, 526, 713, 722
BstV1I GCAGC 4 cut(s) 209, 425, 523, 743
BstX2I RGATCY 2 cut(s) 33, 781
BstYI RGATCY 2 cut(s) 33, 781
Bsu36I CCTNAGG 1 cut(s) 786
BsuRI GGCC 2 cut(s) 639, 655
BtrI CACGTC 1 cut(s) 679
BtsIMutI CAGTG 3 cut(s) 627, 722, 881
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 653
CfoI GCGC 1 cut(s) 689
Cfr13I GGNCC 1 cut(s) 86
Csp6I GTAC 2 cut(s) 356, 646
CviAII CATG 2 cut(s) 107, 319
CviQI GTAC 2 cut(s) 356, 646
DdeI CTNAG 5 cut(s) 544, 601, 714, 786, 894
DpnI GATC 7 cut(s) 35, 63, 105, 741, 783, 834, 875
DpnII GATC 7 cut(s) 33, 61, 103, 739, 781, 832, 873
DraI TTTAAA 1 cut(s) 445
EaeI YGGCCR 1 cut(s) 637
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
Eco47I GGWCC 1 cut(s) 86
Eco72I CACGTG 1 cut(s) 527
Eco81I CCTNAGG 1 cut(s) 786
Eco88I CYCGRG 1 cut(s) 163
Eco91I GGTNACC 1 cut(s) 625
EcoO65I GGTNACC 1 cut(s) 625
FaeI CATG 2 cut(s) 110, 322
FaiI YATR 9 cut(s) 108, 216, 218, 320, 453, 754, 771, 822, 839
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FatI CATG 2 cut(s) 106, 318
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 5 cut(s) 198, 414, 512, 580, 757
Fsp4HI GCNGC 5 cut(s) 198, 414, 512, 580, 757
FspBI CTAG 2 cut(s) 210, 264
GlaI GCGC 1 cut(s) 688
GluI GCNGC 5 cut(s) 198, 414, 512, 580, 757
GsuI CTGGAG 1 cut(s) 876
HaeII RGCGCY 1 cut(s) 690
HaeIII GGCC 2 cut(s) 639, 655
HhaI GCGC 1 cut(s) 689
Hin1II CATG 2 cut(s) 110, 322
Hin6I GCGC 1 cut(s) 687
HinP1I GCGC 1 cut(s) 687
HinfI GANTC 5 cut(s) 19, 137, 260, 479, 560
HphI GGTGA 1 cut(s) 619
Hpy166II GTNNAC 2 cut(s) 392, 524
Hpy188I TCNGA 4 cut(s) 82, 145, 565, 604
Hpy188III TCNNGA 4 cut(s) 23, 165, 682, 855
Hpy8I GTNNAC 2 cut(s) 392, 524
HpyAV CCTTC 2 cut(s) 381, 859
HpyCH4III ACNGT 3 cut(s) 521, 814, 889
HpyCH4IV ACGT 2 cut(s) 526, 678
HpyF10VI GCNNNNNNNGC 7 cut(s) 203, 588, 597, 657, 695, 717, 726
HpyF3I CTNAG 5 cut(s) 544, 601, 714, 786, 894
HpySE526I ACGT 2 cut(s) 526, 678
Hsp92II CATG 2 cut(s) 110, 322
HspAI GCGC 1 cut(s) 687
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 7 cut(s) 33, 61, 103, 739, 781, 832, 873
LguI GCTCTTC 1 cut(s) 485
LmnI GCTCC 2 cut(s) 73, 860
Lsp1109I GCAGC 4 cut(s) 209, 425, 523, 743
LweI GCATC 3 cut(s) 136, 215, 669
MaeI CTAG 2 cut(s) 210, 264
MaeII ACGT 2 cut(s) 526, 678
MaeIII GTNAC 2 cut(s) 625, 816
MalI GATC 7 cut(s) 35, 63, 105, 741, 783, 834, 875
MboI GATC 7 cut(s) 33, 61, 103, 739, 781, 832, 873
MboII GAAGA 6 cut(s) 28, 71, 167, 488, 502, 791
MflI RGATCY 2 cut(s) 33, 781
MhlI GDGCHC 4 cut(s) 394, 526, 713, 722
MlsI TGGCCA 1 cut(s) 639
MluCI AATT 5 cut(s) 238, 440, 446, 484, 568
MluNI TGGCCA 1 cut(s) 639
MlyI GAGTC 1 cut(s) 146
Mox20I TGGCCA 1 cut(s) 639
MscI TGGCCA 1 cut(s) 639
MseI TTAA 8 cut(s) 183, 236, 345, 401, 437, 444, 761, 878
MslI CAYNNNNRTG 1 cut(s) 529
Msp20I TGGCCA 1 cut(s) 639
MspA1I CMGCKG 2 cut(s) 200, 416
MwoI GCNNNNNNNGC 7 cut(s) 203, 588, 597, 657, 695, 717, 726
NdeII GATC 7 cut(s) 33, 61, 103, 739, 781, 832, 873
NlaIII CATG 2 cut(s) 110, 322
NlaIV GGNNCC 2 cut(s) 75, 87
NmuCI GTSAC 1 cut(s) 625
PaeR7I CTCGAG 1 cut(s) 163
PciSI GCTCTTC 1 cut(s) 485
PfeI GAWTC 4 cut(s) 19, 260, 479, 560
PkrI GCNGC 5 cut(s) 199, 415, 513, 581, 758
PleI GAGTC 1 cut(s) 145
PmaCI CACGTG 1 cut(s) 527
PmlI CACGTG 1 cut(s) 527
PpsI GAGTC 1 cut(s) 145
Ppu21I YACGTR 1 cut(s) 527
PspCI CACGTG 1 cut(s) 527
PspEI GGTNACC 1 cut(s) 625
PspN4I GGNNCC 2 cut(s) 75, 87
PspPI GGNCC 1 cut(s) 86
PsuI RGATCY 2 cut(s) 33, 781
PvuII CAGCTG 2 cut(s) 200, 416
RsaI GTAC 2 cut(s) 357, 647
RsaNI GTAC 2 cut(s) 356, 646
RseI CAYNNNNRTG 1 cut(s) 529
SapI GCTCTTC 1 cut(s) 485
SaqAI TTAA 8 cut(s) 183, 236, 345, 401, 437, 444, 761, 878
SatI GCNGC 5 cut(s) 198, 414, 512, 580, 757
Sau3AI GATC 7 cut(s) 33, 61, 103, 739, 781, 832, 873
Sau96I GGNCC 1 cut(s) 86
SchI GAGTC 1 cut(s) 146
SduI GDGCHC 4 cut(s) 394, 526, 713, 722
SfaNI GCATC 3 cut(s) 136, 215, 669
Sfr274I CTCGAG 1 cut(s) 163
SinI GGWCC 1 cut(s) 86
SlaI CTCGAG 1 cut(s) 163
SmiI ATTTAAAT 1 cut(s) 445
SmiMI CAYNNNNRTG 1 cut(s) 529
SmlI CTYRAG 1 cut(s) 163
SmoI CTYRAG 1 cut(s) 163
Sse9I AATT 5 cut(s) 238, 440, 446, 484, 568
SsiI CCGC 2 cut(s) 4, 579
SspMI CTAG 2 cut(s) 210, 264
SwaI ATTTAAAT 1 cut(s) 445
TaaI ACNGT 3 cut(s) 521, 814, 889
TaiI ACGT 2 cut(s) 529, 681
TaqI TCGA 4 cut(s) 164, 230, 362, 422
TasI AATT 5 cut(s) 238, 440, 446, 484, 568
TatI WGTACW 1 cut(s) 645
TauI GCSGC 1 cut(s) 582
TfiI GAWTC 4 cut(s) 19, 260, 479, 560
Tru1I TTAA 8 cut(s) 183, 236, 345, 401, 437, 444, 761, 878
Tru9I TTAA 8 cut(s) 183, 236, 345, 401, 437, 444, 761, 878
TscAI CASTG 3 cut(s) 627, 722, 888
TseFI GTSAC 1 cut(s) 625
TseI GCWGC 4 cut(s) 197, 413, 511, 756
Tsp45I GTSAC 1 cut(s) 625
TspDTI ATGAA 1 cut(s) 387
TspRI CASTG 3 cut(s) 627, 722, 888
VneI GTGCAC 2 cut(s) 390, 522
VpaK11BI GGWCC 1 cut(s) 86
XapI RAATTY 3 cut(s) 238, 440, 568
XcmI CCANNNNNNNNNTGG 1 cut(s) 383
XhoI CTCGAG 1 cut(s) 163
XspI CTAG 2 cut(s) 210, 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.