MD03G1186200.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015574
Physical Location & Seq
Forward (+)
25377449 .. 25377847
399 bp
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UTR
Exon/CDS
Intron
MD03G1186200.v1.1.491

Sequence Viewer

Length: 399 bp
ATGATAGCATTAGATCATCATTATCAAAATATCATGAGTGCAGTTGAATTTGTGGACTACTTTCCATCCATGATTGCAAGGATGGGAGACGAGGGGTTTATCGGGGAGCTCTGCAGTGGGTTTCGATTGCTGATGGATGTCGAGAAGGGGCTCATCACTTTTGAAAGCTTGAAGAGGAACTGCGTTCTGCTGGGATTGTATGACATGGGAGATGATGAGCTTCTGTGGATGTTGATGGAGGGTGATTTGGATGGAGATGGAGCTCTCAGTCAGATGGAGTTTTGTATTCTCATGTTTAGGTTAAGTCCAGGGATAATGGATGGATCTAAGCAATTTTGGATGGAAGACTTCGATCACCGTCGTGTAAATGATCATCAAATGTATGTAGGCTTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

133

Amino Acids

15.28

Weight (kDa)

4.33

Isoelectric Point (pI)

45.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 48 - 100 1e-15 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015574)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21240
malus_domestica MD03G1186200.v1.1 MD11G1233900.v1.1
prunus_persica Prupe.4G192800_v2.0.a1
pyrus_communis pycom11g20610
rosa_chinensis RchiOBHm_Chr5g0036321
rosa_laevigata RLG00000033686
rosa_multiflora Rmu_sc0000247.1_g000078
rosa_roxburghii Rroxscaffold_1G00044470
rosa_rugosa Rorug05G0155100
rosa_samantha Rh5AG248400 Rh5BG249200 Rh5CG280500 Rh5DG257700
rosa_wichuraiana Rw0G002060 Rw5G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 331
AcsI RAATTY 1 cut(s) 47
AgsI TTSAA 3 cut(s) 47, 164, 172
AjnI CCWGG 1 cut(s) 307
AleI CACNNNNGTG 1 cut(s) 360
AluBI AGCT 4 cut(s) 109, 168, 220, 263
AluI AGCT 4 cut(s) 109, 168, 220, 263
Alw21I GWGCWC 2 cut(s) 111, 265
Alw26I GTCTC 1 cut(s) 81
AlwI GGATC 1 cut(s) 331
ApoI RAATTY 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 254, 347
BanII GRGCYC 3 cut(s) 111, 153, 265
BarI GAAGNNNNNNTAC 1 cut(s) 374
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 2 cut(s) 111, 265
BccI CCATC 9 cut(s) 73, 76, 127, 229, 245, 251, 268, 314, 334
BciT130I CCWGG 1 cut(s) 309
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 1 cut(s) 81
BfmI CTRYAG 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 309
BmrFI CCNGG 1 cut(s) 309
BpiI GAAGAC 1 cut(s) 351
BsaJI CCNNGG 1 cut(s) 308
BseBI CCWGG 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 7 cut(s) 65, 87, 142, 234, 256, 325, 345
BseMII CTCAG 1 cut(s) 280
BseYI CCCAGC 1 cut(s) 190
BsgI GTGCAG 1 cut(s) 60
BsiHKAI GWGCWC 2 cut(s) 111, 265
BsmAI GTCTC 1 cut(s) 81
BsmBI CGTCTC 1 cut(s) 81
Bsp1286I GDGCHC 3 cut(s) 111, 153, 265
Bsp143I GATC 4 cut(s) 13, 323, 352, 370
BspCNI CTCAG 1 cut(s) 279
BspHI TCATGA 1 cut(s) 33
BspMAI CTGCAG 1 cut(s) 116
BspPI GGATC 1 cut(s) 331
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 4 cut(s) 13, 323, 352, 370
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 359
Bst6I CTCTTC 1 cut(s) 167
BstDEI CTNAG 2 cut(s) 266, 327
BstF5I GGATG 7 cut(s) 65, 87, 142, 234, 256, 325, 345
BstKTI GATC 4 cut(s) 16, 326, 355, 373
BstMAI GTCTC 1 cut(s) 81
BstMBI GATC 4 cut(s) 13, 323, 352, 370
BstNI CCWGG 1 cut(s) 309
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 1 cut(s) 112
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 323
BstYI RGATCY 1 cut(s) 323
BtsCI GGATG 7 cut(s) 65, 87, 142, 234, 256, 325, 345
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 1 cut(s) 121
CciI TCATGA 1 cut(s) 33
CviAII CATG 4 cut(s) 34, 70, 205, 292
CviJI RGCY 6 cut(s) 109, 151, 168, 220, 263, 390
CviKI_1 RGCY 6 cut(s) 109, 151, 168, 220, 263, 390
DdeI CTNAG 2 cut(s) 266, 327
DpnI GATC 4 cut(s) 15, 325, 354, 372
DpnII GATC 4 cut(s) 13, 323, 352, 370
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
Ecl136II GAGCTC 2 cut(s) 109, 263
Eco24I GRGCYC 3 cut(s) 111, 153, 265
Eco53kI GAGCTC 2 cut(s) 109, 263
EcoICRI GAGCTC 2 cut(s) 109, 263
EcoRII CCWGG 1 cut(s) 307
EcoT38I GRGCYC 3 cut(s) 111, 153, 265
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 4 cut(s) 37, 73, 208, 295
FaiI YATR 6 cut(s) 35, 71, 201, 206, 293, 384
FatI CATG 4 cut(s) 33, 69, 204, 291
FbaI TGATCA 1 cut(s) 370
FokI GGATG 7 cut(s) 52, 94, 149, 241, 263, 332, 352
FriOI GRGCYC 3 cut(s) 111, 153, 265
GsaI CCCAGC 1 cut(s) 194
Hin1II CATG 4 cut(s) 37, 73, 208, 295
HindIII AAGCTT 1 cut(s) 166
HphI GGTGA 2 cut(s) 254, 347
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 1 cut(s) 273
Hpy188III TCNNGA 2 cut(s) 34, 142
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 1 cut(s) 363
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4V TGCA 3 cut(s) 41, 77, 114
HpyF3I CTNAG 2 cut(s) 266, 327
Hsp92II CATG 4 cut(s) 37, 73, 208, 295
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 4 cut(s) 13, 323, 352, 370
LmnI GCTCC 2 cut(s) 106, 260
LpnPI CCDG 3 cut(s) 176, 294, 321
MalI GATC 4 cut(s) 15, 325, 354, 372
MboI GATC 4 cut(s) 13, 323, 352, 370
MboII GAAGA 2 cut(s) 184, 356
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 3 cut(s) 111, 153, 265
MluCI AATT 2 cut(s) 47, 332
MnlI CCTC 3 cut(s) 85, 168, 232
MseI TTAA 1 cut(s) 302
MslI CAYNNNNRTG 1 cut(s) 360
MspR9I CCNGG 1 cut(s) 309
MvaI CCWGG 1 cut(s) 309
NdeII GATC 4 cut(s) 13, 323, 352, 370
NlaIII CATG 4 cut(s) 37, 73, 208, 295
OliI CACNNNNGTG 1 cut(s) 360
PagI TCATGA 1 cut(s) 33
Psp124BI GAGCTC 2 cut(s) 111, 265
Psp6I CCWGG 1 cut(s) 307
PspFI CCCAGC 1 cut(s) 190
PspGI CCWGG 1 cut(s) 307
PstI CTGCAG 1 cut(s) 116
PsuI RGATCY 1 cut(s) 323
RseI CAYNNNNRTG 1 cut(s) 360
SacI GAGCTC 2 cut(s) 111, 265
SaqAI TTAA 1 cut(s) 302
Sau3AI GATC 4 cut(s) 13, 323, 352, 370
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 3 cut(s) 111, 153, 265
SetI ASST 5 cut(s) 111, 170, 222, 265, 302
SfcI CTRYAG 1 cut(s) 112
SmiMI CAYNNNNRTG 1 cut(s) 360
Sse9I AATT 2 cut(s) 47, 332
SstI GAGCTC 2 cut(s) 111, 265
StyD4I CCNGG 1 cut(s) 307
TaaI ACNGT 1 cut(s) 359
TaqI TCGA 3 cut(s) 124, 141, 351
TasI AATT 2 cut(s) 47, 332
Tru1I TTAA 1 cut(s) 302
Tru9I TTAA 1 cut(s) 302
TscAI CASTG 1 cut(s) 121
TspRI CASTG 1 cut(s) 121
XapI RAATTY 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.