Rw0G002060

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00065
OG0015574
Physical Location & Seq
Reverse (-)
4172 .. 4447
276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G002060.1

Sequence Viewer

Length: 276 bp
ATGGATGTGGAGAAAGGGCTCATCACGTTCGAAAGCTTGAAGCGGAACAGCCTCTTGCTGGGGATTCATGACATGGGGGACGATGAGCTTGTGTGGATGTTGATGGAAGGTGATCTCGATGGAGACGGGGCTCTCAGCCAGTTGGAGTTTTGCATTCTCATGTTTAGGTTAAGTCCAGGTCTGATGGATGCAGGACCTAATAAGCAGGTTTGGATGGAGGACATTGATCGTCGTCGTCGTCATGTAAATGATCAGATACATGTAGGGTTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

91

Amino Acids

10.46

Weight (kDa)

4.58

Isoelectric Point (pI)

57.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 4 - 56 6.9e-16 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015574)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21240
malus_domestica MD03G1186200.v1.1 MD11G1233900.v1.1
prunus_persica Prupe.4G192800_v2.0.a1
pyrus_communis pycom11g20610
rosa_chinensis RchiOBHm_Chr5g0036321
rosa_laevigata RLG00000033686
rosa_multiflora Rmu_sc0000247.1_g000078
rosa_roxburghii Rroxscaffold_1G00044470
rosa_rugosa Rorug05G0155100
rosa_samantha Rh5AG248400 Rh5BG249200 Rh5CG280500 Rh5DG257700
rosa_wichuraiana Rw0G002060 Rw5G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AciI CCGC 1 cut(s) 43
AfiI CCNNNNNNNGG 1 cut(s) 58
AflIII ACRYGT 1 cut(s) 259
AgsI TTSAA 2 cut(s) 40, 272
AjnI CCWGG 1 cut(s) 175
AluBI AGCT 2 cut(s) 36, 88
AluI AGCT 2 cut(s) 36, 88
Alw26I GTCTC 1 cut(s) 117
AspS9I GGNCC 1 cut(s) 194
AsuHPI GGTGA 1 cut(s) 122
AsuII TTCGAA 1 cut(s) 30
AvaII GGWCC 1 cut(s) 194
BanII GRGCYC 2 cut(s) 21, 133
BccI CCATC 4 cut(s) 97, 113, 178, 208
BciT130I CCWGG 1 cut(s) 177
BclI TGATCA 1 cut(s) 250
BcoDI GTCTC 1 cut(s) 117
BfuAI ACCTGC 1 cut(s) 196
Bme1390I CCNGG 1 cut(s) 177
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 1 cut(s) 194
BmrFI CCNGG 1 cut(s) 177
BmsI GCATC 1 cut(s) 178
Bpu14I TTCGAA 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 1 cut(s) 177
BseGI GGATG 4 cut(s) 10, 102, 193, 219
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMII CTCAG 1 cut(s) 148
BseNI ACTGG 1 cut(s) 139
BseYI CCCAGC 1 cut(s) 58
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 1 cut(s) 117
BsmBI CGTCTC 1 cut(s) 117
BsmFI GGGAC 1 cut(s) 92
BsmI GAATGC 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 30
Bsp1286I GDGCHC 2 cut(s) 21, 133
Bsp143I GATC 3 cut(s) 112, 226, 250
BspACI CCGC 1 cut(s) 43
BspCNI CTCAG 1 cut(s) 147
BspHI TCATGA 1 cut(s) 67
BspMI ACCTGC 1 cut(s) 196
BspT104I TTCGAA 1 cut(s) 30
BsrI ACTGG 1 cut(s) 139
BssMI GATC 3 cut(s) 112, 226, 250
Bst2UI CCWGG 1 cut(s) 177
BstBI TTCGAA 1 cut(s) 30
BstDEI CTNAG 1 cut(s) 134
BstF5I GGATG 4 cut(s) 10, 102, 193, 219
BstKTI GATC 3 cut(s) 115, 229, 253
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 3 cut(s) 112, 226, 250
BstNI CCWGG 1 cut(s) 177
BstNSI RCATGY 1 cut(s) 263
BstSCI CCNGG 1 cut(s) 175
BtsCI GGATG 4 cut(s) 10, 102, 193, 219
BveI ACCTGC 1 cut(s) 196
CciI TCATGA 1 cut(s) 67
Cfr13I GGNCC 1 cut(s) 194
CviAII CATG 5 cut(s) 68, 73, 160, 242, 260
CviJI RGCY 6 cut(s) 19, 36, 51, 88, 131, 138
CviKI_1 RGCY 6 cut(s) 19, 36, 51, 88, 131, 138
DdeI CTNAG 1 cut(s) 134
DpnI GATC 3 cut(s) 114, 228, 252
DpnII GATC 3 cut(s) 112, 226, 250
Eco24I GRGCYC 2 cut(s) 21, 133
Eco47I GGWCC 1 cut(s) 194
EcoO109I RGGNCCY 1 cut(s) 194
EcoRII CCWGG 1 cut(s) 175
EcoT38I GRGCYC 2 cut(s) 21, 133
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 5 cut(s) 71, 76, 163, 245, 263
FaiI YATR 5 cut(s) 69, 74, 161, 243, 261
FaqI GGGAC 1 cut(s) 92
FatI CATG 5 cut(s) 67, 72, 159, 241, 259
FbaI TGATCA 1 cut(s) 250
FokI GGATG 4 cut(s) 17, 109, 200, 226
FriOI GRGCYC 2 cut(s) 21, 133
GsaI CCCAGC 1 cut(s) 62
Hin1II CATG 5 cut(s) 71, 76, 163, 245, 263
HindIII AAGCTT 1 cut(s) 34
HinfI GANTC 1 cut(s) 64
HphI GGTGA 1 cut(s) 122
Hpy188I TCNGA 2 cut(s) 183, 255
Hpy188III TCNNGA 2 cut(s) 68, 116
Hpy99I CGWCG 3 cut(s) 234, 237, 240
HpyAV CCTTC 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 26
HpyCH4V TGCA 2 cut(s) 153, 191
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 1 cut(s) 26
Hsp92II CATG 5 cut(s) 71, 76, 163, 245, 263
Ksp22I TGATCA 1 cut(s) 250
Kzo9I GATC 3 cut(s) 112, 226, 250
LpnPI CCDG 6 cut(s) 44, 152, 162, 177, 189, 191
LweI GCATC 1 cut(s) 178
MaeII ACGT 1 cut(s) 26
MalI GATC 3 cut(s) 114, 228, 252
MboI GATC 3 cut(s) 112, 226, 250
MhlI GDGCHC 2 cut(s) 21, 133
MmeI TCCRAC 1 cut(s) 123
MnlI CCTC 2 cut(s) 62, 211
MseI TTAA 1 cut(s) 170
MslI CAYNNNNRTG 2 cut(s) 158, 246
MspR9I CCNGG 1 cut(s) 177
Mva1269I GAATGC 1 cut(s) 153
MvaI CCWGG 1 cut(s) 177
NdeII GATC 3 cut(s) 112, 226, 250
NlaIII CATG 5 cut(s) 71, 76, 163, 245, 263
NspI RCATGY 1 cut(s) 263
NspV TTCGAA 1 cut(s) 30
PagI TCATGA 1 cut(s) 67
PciI ACATGT 1 cut(s) 259
PcsI WCGNNNNNNNCGW 1 cut(s) 235
PctI GAATGC 1 cut(s) 153
PfeI GAWTC 1 cut(s) 64
PpuMI RGGWCCY 1 cut(s) 194
PscI ACATGT 1 cut(s) 259
Psp5II RGGWCCY 1 cut(s) 194
Psp6I CCWGG 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 58
PspGI CCWGG 1 cut(s) 175
PspPI GGNCC 1 cut(s) 194
PspPPI RGGWCCY 1 cut(s) 194
RseI CAYNNNNRTG 2 cut(s) 158, 246
SaqAI TTAA 1 cut(s) 170
Sau3AI GATC 3 cut(s) 112, 226, 250
Sau96I GGNCC 1 cut(s) 194
ScrFI CCNGG 1 cut(s) 177
SduI GDGCHC 2 cut(s) 21, 133
SetI ASST 8 cut(s) 29, 38, 90, 112, 170, 181, 199, 210
SfaNI GCATC 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 30
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 2 cut(s) 158, 246
SsiI CCGC 1 cut(s) 43
StyD4I CCNGG 1 cut(s) 175
TaiI ACGT 1 cut(s) 29
TaqI TCGA 2 cut(s) 30, 117
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 1 cut(s) 170
Tru9I TTAA 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 194
XceI RCATGY 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.