Prupe.4G192800_v2.0.a1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0015574
Physical Location & Seq
Reverse (-)
11606234 .. 11607418
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G192800.1

Sequence Viewer

Length: 390 bp
ATGATATCGTTAGATCATCACCACATGATGAGCGCAGTTGAATTTGTGGACTATTTTCCGTCCATGATCGCGAGGCTGGGAGAAGAAGGGTTTATTGGGGAGCTCTGCAATGGGTTTGTTTTGCTGATGGATGTGGAGAAGGGGCTCATCACCTTTGAGAGCTTGAAGAGGAACTGCGTTGTGCTGGGCTTGCATGACATGGGAGATGATGAGCTTGTGTGGATGTTGATGGAGGGTGATTTGGATGGAGATGGGGCCCTCAGTCAGCTGGAGTTTTGCATCCTCATGTTTAGGTTAAGTCCAGGTTTGATGGATGGATCTAAGCAGCTGTGGAAGGAAAACTTTGATCATCCTCCTCATCATCAAGTACATGTAGGAGGCTTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

130

Amino Acids

14.6

Weight (kDa)

4.63

Isoelectric Point (pI)

30.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015574)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21240
malus_domestica MD03G1186200.v1.1 MD11G1233900.v1.1
prunus_persica Prupe.4G192800_v2.0.a1
pyrus_communis pycom11g20610
rosa_chinensis RchiOBHm_Chr5g0036321
rosa_laevigata RLG00000033686
rosa_multiflora Rmu_sc0000247.1_g000078
rosa_roxburghii Rroxscaffold_1G00044470
rosa_rugosa Rorug05G0155100
rosa_samantha Rh5AG248400 Rh5BG249200 Rh5CG280500 Rh5DG257700
rosa_wichuraiana Rw0G002060 Rw5G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 71
AclWI GGATC 1 cut(s) 325
AcsI RAATTY 1 cut(s) 41
AfaI GTAC 1 cut(s) 369
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 2 cut(s) 41, 166
AjnI CCWGG 1 cut(s) 301
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 5 cut(s) 103, 162, 214, 268, 328
AluI AGCT 5 cut(s) 103, 162, 214, 268, 328
Alw21I GWGCWC 1 cut(s) 105
AlwI GGATC 1 cut(s) 325
AoxI GGCC 1 cut(s) 255
ApaI GGGCCC 1 cut(s) 259
ApeKI GCWGC 1 cut(s) 325
ApoI RAATTY 1 cut(s) 41
AspLEI GCGC 1 cut(s) 35
AspS9I GGNCC 2 cut(s) 255, 256
AsuHPI GGTGA 3 cut(s) 11, 142, 248
BaeGI GKGCMC 1 cut(s) 259
BanII GRGCYC 3 cut(s) 105, 147, 259
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 337
BccI CCATC 6 cut(s) 121, 223, 239, 245, 304, 308
BciT130I CCWGG 1 cut(s) 303
BclI TGATCA 1 cut(s) 346
BisI GCNGC 1 cut(s) 326
BlsI GCNGC 1 cut(s) 327
Bme1390I CCNGG 1 cut(s) 303
BmgT120I GGNCC 2 cut(s) 255, 256
BmiI GGNNCC 2 cut(s) 256, 257
BmrFI CCNGG 1 cut(s) 303
BmsI GCATC 1 cut(s) 288
BpmI CTGGAG 1 cut(s) 290
Bse3DI GCAATG 1 cut(s) 115
BseBI CCWGG 1 cut(s) 303
BseGI GGATG 6 cut(s) 136, 228, 250, 279, 319, 349
BseMI GCAATG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 274
BseRI GAGGAG 1 cut(s) 345
BseSI GKGCMC 1 cut(s) 259
BseXI GCAGC 1 cut(s) 337
BseYI CCCAGC 2 cut(s) 76, 184
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 1 cut(s) 257
BsiHKAI GWGCWC 1 cut(s) 105
BsnI GGCC 1 cut(s) 257
Bsp120I GGGCCC 1 cut(s) 255
Bsp1286I GDGCHC 3 cut(s) 105, 147, 259
Bsp143I GATC 4 cut(s) 13, 66, 317, 346
Bsp68I TCGCGA 1 cut(s) 71
BspANI GGCC 1 cut(s) 257
BspCNI CTCAG 1 cut(s) 273
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 256, 257
BspPI GGATC 1 cut(s) 325
BsrDI GCAATG 1 cut(s) 115
BssMI GATC 4 cut(s) 13, 66, 317, 346
Bst2UI CCWGG 1 cut(s) 303
Bst6I CTCTTC 1 cut(s) 161
BstC8I GCNNGC 1 cut(s) 191
BstDEI CTNAG 2 cut(s) 260, 321
BstF5I GGATG 6 cut(s) 136, 228, 250, 279, 319, 349
BstFNI CGCG 1 cut(s) 71
BstHHI GCGC 1 cut(s) 35
BstKTI GATC 4 cut(s) 16, 69, 320, 349
BstMBI GATC 4 cut(s) 13, 66, 317, 346
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstNI CCWGG 1 cut(s) 303
BstNSI RCATGY 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 301
BstSLI GKGCMC 1 cut(s) 259
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 1 cut(s) 337
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BsuRI GGCC 1 cut(s) 257
BtsCI GGATG 6 cut(s) 136, 228, 250, 279, 319, 349
BtuMI TCGCGA 1 cut(s) 71
Cac8I GCNNGC 1 cut(s) 191
CfoI GCGC 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 255, 256
Csp6I GTAC 1 cut(s) 368
CviAII CATG 6 cut(s) 25, 64, 194, 199, 286, 371
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 2 cut(s) 260, 321
DpnI GATC 4 cut(s) 15, 68, 319, 348
DpnII GATC 4 cut(s) 13, 66, 317, 346
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
Ecl136II GAGCTC 1 cut(s) 103
Eco24I GRGCYC 3 cut(s) 105, 147, 259
Eco32I GATATC 1 cut(s) 6
Eco53kI GAGCTC 1 cut(s) 103
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 2 cut(s) 255, 256
EcoRII CCWGG 1 cut(s) 301
EcoRV GATATC 1 cut(s) 6
EcoT38I GRGCYC 3 cut(s) 105, 147, 259
FaeI CATG 6 cut(s) 28, 67, 197, 202, 289, 374
FaiI YATR 6 cut(s) 26, 65, 195, 200, 287, 372
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FatI CATG 6 cut(s) 24, 63, 193, 198, 285, 370
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 326
FokI GGATG 6 cut(s) 143, 235, 257, 266, 326, 336
FriOI GRGCYC 3 cut(s) 105, 147, 259
Fsp4HI GCNGC 1 cut(s) 326
GlaI GCGC 1 cut(s) 34
GluI GCNGC 1 cut(s) 326
GsaI CCCAGC 2 cut(s) 80, 188
GsuI CTGGAG 1 cut(s) 290
HaeIII GGCC 1 cut(s) 257
HhaI GCGC 1 cut(s) 35
Hin1II CATG 6 cut(s) 28, 67, 197, 202, 289, 374
Hin6I GCGC 1 cut(s) 33
HinP1I GCGC 1 cut(s) 33
HphI GGTGA 3 cut(s) 11, 142, 248
Hpy166II GTNNAC 1 cut(s) 49
Hpy188III TCNNGA 1 cut(s) 70
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 3 cut(s) 80, 133, 328
HpyCH4V TGCA 3 cut(s) 108, 193, 279
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpyF3I CTNAG 2 cut(s) 260, 321
Hsp92II CATG 6 cut(s) 28, 67, 197, 202, 289, 374
HspAI GCGC 1 cut(s) 33
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 4 cut(s) 13, 66, 317, 346
LmnI GCTCC 1 cut(s) 100
LpnPI CCDG 5 cut(s) 62, 170, 254, 288, 315
Lsp1109I GCAGC 1 cut(s) 337
LweI GCATC 1 cut(s) 288
MalI GATC 4 cut(s) 15, 68, 319, 348
MboI GATC 4 cut(s) 13, 66, 317, 346
MboII GAAGA 2 cut(s) 95, 178
MflI RGATCY 1 cut(s) 317
MhlI GDGCHC 3 cut(s) 105, 147, 259
MluCI AATT 1 cut(s) 41
MnlI CCTC 8 cut(s) 66, 162, 226, 269, 293, 363, 366, 371
MseI TTAA 1 cut(s) 296
MslI CAYNNNNRTG 1 cut(s) 284
MspA1I CMGCKG 2 cut(s) 268, 328
MspR9I CCNGG 1 cut(s) 303
MvaI CCWGG 1 cut(s) 303
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 1 cut(s) 190
NdeII GATC 4 cut(s) 13, 66, 317, 346
NlaIII CATG 6 cut(s) 28, 67, 197, 202, 289, 374
NlaIV GGNNCC 2 cut(s) 256, 257
NruI TCGCGA 1 cut(s) 71
NspI RCATGY 1 cut(s) 374
PciI ACATGT 1 cut(s) 370
PkrI GCNGC 1 cut(s) 327
PscI ACATGT 1 cut(s) 370
Psp124BI GAGCTC 1 cut(s) 105
Psp6I CCWGG 1 cut(s) 301
PspFI CCCAGC 2 cut(s) 76, 184
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 2 cut(s) 256, 257
PspOMI GGGCCC 1 cut(s) 255
PspPI GGNCC 2 cut(s) 255, 256
PsuI RGATCY 1 cut(s) 317
PvuII CAGCTG 2 cut(s) 268, 328
RruI TCGCGA 1 cut(s) 71
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
RseI CAYNNNNRTG 1 cut(s) 284
SacI GAGCTC 1 cut(s) 105
SaqAI TTAA 1 cut(s) 296
SatI GCNGC 1 cut(s) 326
Sau3AI GATC 4 cut(s) 13, 66, 317, 346
Sau96I GGNCC 2 cut(s) 255, 256
ScrFI CCNGG 1 cut(s) 303
SduI GDGCHC 3 cut(s) 105, 147, 259
SetI ASST 8 cut(s) 105, 155, 164, 216, 270, 296, 307, 330
SfaNI GCATC 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 284
Sse9I AATT 1 cut(s) 41
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 301
TasI AATT 1 cut(s) 41
TatI WGTACW 1 cut(s) 367
Tru1I TTAA 1 cut(s) 296
Tru9I TTAA 1 cut(s) 296
TseI GCWGC 1 cut(s) 325
TspGWI ACGGA 1 cut(s) 48
XapI RAATTY 1 cut(s) 41
XceI RCATGY 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.