Rh5DG257700

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0015574
Physical Location & Seq
Reverse (-)
30472196 .. 30473267
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG257700.1

Sequence Viewer

Length: 408 bp
ATGATGGCCACAGTACTAGAGCATCACATCAATAACGCCGCAGTTGAGTTCGTGGACTACTTTCCTTCCATGATTCTGCGGCTCGGAGAAGAAGCGTTCATCGCAGAGCTGTGCAGCGGGTTTCGGCTGCTGATGGATGTGGAGAAAGGGCTCATCACGTTCGAAAGCTTGAAGCGGAACAGCCTCTTGCTGGGGATTCATGACATGGGGGACGATGAGCTTGTGTGGATGTTGATGGAAGGTGATCTCGATGGAGACGGGGCTCTCAGCCAGTTGGAGTTTTGCATTCTCATGTTTAGGTTAAGTCCAGGTCTGATGGATGCAGGACCTAATAAGCAGGTTTGGATGGAGGACATTGATCGTCGTCGTCGTCATGTAAATGATCAGATACATGTAGGGTTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

135

Amino Acids

15.44

Weight (kDa)

4.61

Isoelectric Point (pI)

51.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 48 - 100 1.9e-15 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015574)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21240
malus_domestica MD03G1186200.v1.1 MD11G1233900.v1.1
prunus_persica Prupe.4G192800_v2.0.a1
pyrus_communis pycom11g20610
rosa_chinensis RchiOBHm_Chr5g0036321
rosa_laevigata RLG00000033686
rosa_multiflora Rmu_sc0000247.1_g000078
rosa_roxburghii Rroxscaffold_1G00044470
rosa_rugosa Rorug05G0155100
rosa_samantha Rh5AG248400 Rh5BG249200 Rh5CG280500 Rh5DG257700
rosa_wichuraiana Rw0G002060 Rw5G022810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 328
AciI CCGC 4 cut(s) 39, 79, 117, 175
AcoI YGGCCR 1 cut(s) 6
AfaI GTAC 1 cut(s) 15
AfiI CCNNNNNNNGG 1 cut(s) 190
AflIII ACRYGT 1 cut(s) 391
AgsI TTSAA 2 cut(s) 172, 404
AjnI CCWGG 1 cut(s) 307
AluBI AGCT 3 cut(s) 109, 168, 220
AluI AGCT 3 cut(s) 109, 168, 220
Alw26I GTCTC 1 cut(s) 249
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 2 cut(s) 114, 127
AspS9I GGNCC 1 cut(s) 326
AsuHPI GGTGA 1 cut(s) 254
AsuII TTCGAA 1 cut(s) 162
AvaII GGWCC 1 cut(s) 326
BalI TGGCCA 1 cut(s) 8
BanII GRGCYC 2 cut(s) 153, 265
BbvI GCAGC 2 cut(s) 114, 126
BccI CCATC 5 cut(s) 127, 229, 245, 310, 340
BciT130I CCWGG 1 cut(s) 309
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 1 cut(s) 249
BfaI CTAG 1 cut(s) 17
BfuAI ACCTGC 1 cut(s) 328
BisI GCNGC 4 cut(s) 39, 80, 115, 128
BlsI GCNGC 4 cut(s) 40, 81, 116, 129
BmcAI AGTACT 1 cut(s) 15
Bme1390I CCNGG 1 cut(s) 309
Bme18I GGWCC 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 326
BmrFI CCNGG 1 cut(s) 309
BmsI GCATC 2 cut(s) 31, 310
Bpu14I TTCGAA 1 cut(s) 162
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 271
BseBI CCWGG 1 cut(s) 309
BseGI GGATG 4 cut(s) 142, 234, 325, 351
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 1 cut(s) 280
BseNI ACTGG 1 cut(s) 271
BseXI GCAGC 2 cut(s) 114, 126
BseYI CCCAGC 1 cut(s) 190
BsgI GTGCAG 1 cut(s) 133
BshFI GGCC 1 cut(s) 8
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 1 cut(s) 190
BsmAI GTCTC 1 cut(s) 249
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 285
BsnI GGCC 1 cut(s) 8
Bsp119I TTCGAA 1 cut(s) 162
Bsp1286I GDGCHC 2 cut(s) 153, 265
Bsp143I GATC 3 cut(s) 244, 358, 382
BspACI CCGC 4 cut(s) 39, 79, 117, 175
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 279
BspHI TCATGA 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 328
BspT104I TTCGAA 1 cut(s) 162
BsrI ACTGG 1 cut(s) 271
BssMI GATC 3 cut(s) 244, 358, 382
Bst2UI CCWGG 1 cut(s) 309
Bst4CI ACNGT 1 cut(s) 13
BstBI TTCGAA 1 cut(s) 162
BstDEI CTNAG 1 cut(s) 266
BstF5I GGATG 4 cut(s) 142, 234, 325, 351
BstKTI GATC 3 cut(s) 247, 361, 385
BstMAI GTCTC 1 cut(s) 249
BstMBI GATC 3 cut(s) 244, 358, 382
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstNI CCWGG 1 cut(s) 309
BstNSI RCATGY 1 cut(s) 395
BstSCI CCNGG 1 cut(s) 307
BstV1I GCAGC 2 cut(s) 114, 126
BsuRI GGCC 1 cut(s) 8
BtgZI GCGATG 1 cut(s) 85
BtsCI GGATG 4 cut(s) 142, 234, 325, 351
BveI ACCTGC 1 cut(s) 328
CciI TCATGA 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 14
CviAII CATG 6 cut(s) 70, 200, 205, 292, 374, 392
CviQI GTAC 1 cut(s) 14
DdeI CTNAG 1 cut(s) 266
DpnI GATC 3 cut(s) 246, 360, 384
DpnII GATC 3 cut(s) 244, 358, 382
EaeI YGGCCR 1 cut(s) 6
Eco24I GRGCYC 2 cut(s) 153, 265
Eco47I GGWCC 1 cut(s) 326
EcoO109I RGGNCCY 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 307
EcoT38I GRGCYC 2 cut(s) 153, 265
Esp3I CGTCTC 1 cut(s) 249
FaeI CATG 6 cut(s) 73, 203, 208, 295, 377, 395
FaiI YATR 6 cut(s) 71, 201, 206, 293, 375, 393
FaqI GGGAC 1 cut(s) 224
FatI CATG 6 cut(s) 69, 199, 204, 291, 373, 391
FauI CCCGC 1 cut(s) 110
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 4 cut(s) 39, 80, 115, 128
FokI GGATG 4 cut(s) 149, 241, 332, 358
FriOI GRGCYC 2 cut(s) 153, 265
Fsp4HI GCNGC 4 cut(s) 39, 80, 115, 128
FspBI CTAG 1 cut(s) 17
GluI GCNGC 4 cut(s) 39, 80, 115, 128
GsaI CCCAGC 1 cut(s) 194
HaeIII GGCC 1 cut(s) 8
Hin1II CATG 6 cut(s) 73, 203, 208, 295, 377, 395
HindIII AAGCTT 1 cut(s) 166
HinfI GANTC 2 cut(s) 73, 196
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 3 cut(s) 86, 315, 387
Hpy188III TCNNGA 2 cut(s) 200, 248
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 3 cut(s) 366, 369, 372
HpyAV CCTTC 2 cut(s) 75, 233
HpyCH4III ACNGT 1 cut(s) 13
HpyCH4IV ACGT 1 cut(s) 158
HpyCH4V TGCA 3 cut(s) 114, 285, 323
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
HpyF3I CTNAG 1 cut(s) 266
HpySE526I ACGT 1 cut(s) 158
Hsp92II CATG 6 cut(s) 73, 203, 208, 295, 377, 395
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 3 cut(s) 244, 358, 382
LpnPI CCDG 6 cut(s) 176, 284, 294, 309, 321, 323
Lsp1109I GCAGC 2 cut(s) 114, 126
LweI GCATC 2 cut(s) 31, 310
MaeI CTAG 1 cut(s) 17
MaeII ACGT 1 cut(s) 158
MalI GATC 3 cut(s) 246, 360, 384
MboI GATC 3 cut(s) 244, 358, 382
MboII GAAGA 1 cut(s) 101
MhlI GDGCHC 2 cut(s) 153, 265
MlsI TGGCCA 1 cut(s) 8
MluNI TGGCCA 1 cut(s) 8
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 2 cut(s) 194, 343
Mox20I TGGCCA 1 cut(s) 8
MscI TGGCCA 1 cut(s) 8
MseI TTAA 1 cut(s) 302
MslI CAYNNNNRTG 2 cut(s) 290, 378
Msp20I TGGCCA 1 cut(s) 8
MspA1I CMGCKG 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 285
MvaI CCWGG 1 cut(s) 309
MwoI GCNNNNNNNGC 1 cut(s) 101
NdeII GATC 3 cut(s) 244, 358, 382
NlaIII CATG 6 cut(s) 73, 203, 208, 295, 377, 395
NspI RCATGY 1 cut(s) 395
NspV TTCGAA 1 cut(s) 162
PagI TCATGA 1 cut(s) 199
PciI ACATGT 1 cut(s) 391
PcsI WCGNNNNNNNCGW 1 cut(s) 367
PctI GAATGC 1 cut(s) 285
PfeI GAWTC 2 cut(s) 73, 196
PkrI GCNGC 4 cut(s) 40, 81, 116, 129
PpuMI RGGWCCY 1 cut(s) 326
PscI ACATGT 1 cut(s) 391
Psp5II RGGWCCY 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 307
PspFI CCCAGC 1 cut(s) 190
PspGI CCWGG 1 cut(s) 307
PspPI GGNCC 1 cut(s) 326
PspPPI RGGWCCY 1 cut(s) 326
RsaI GTAC 1 cut(s) 15
RsaNI GTAC 1 cut(s) 14
RseI CAYNNNNRTG 2 cut(s) 290, 378
SaqAI TTAA 1 cut(s) 302
SatI GCNGC 4 cut(s) 39, 80, 115, 128
Sau3AI GATC 3 cut(s) 244, 358, 382
Sau96I GGNCC 1 cut(s) 326
ScaI AGTACT 1 cut(s) 15
ScrFI CCNGG 1 cut(s) 309
SduI GDGCHC 2 cut(s) 153, 265
SetI ASST 9 cut(s) 111, 161, 170, 222, 244, 302, 313, 331, 342
SfaNI GCATC 2 cut(s) 31, 310
SfuI TTCGAA 1 cut(s) 162
SinI GGWCC 1 cut(s) 326
SmiMI CAYNNNNRTG 2 cut(s) 290, 378
SsiI CCGC 4 cut(s) 39, 79, 117, 175
SspMI CTAG 1 cut(s) 17
StyD4I CCNGG 1 cut(s) 307
TaaI ACNGT 1 cut(s) 13
TaiI ACGT 1 cut(s) 161
TaqI TCGA 2 cut(s) 162, 249
TatI WGTACW 1 cut(s) 13
TauI GCSGC 2 cut(s) 41, 82
TfiI GAWTC 2 cut(s) 73, 196
Tru1I TTAA 1 cut(s) 302
Tru9I TTAA 1 cut(s) 302
TseI GCWGC 2 cut(s) 114, 127
TspDTI ATGAA 2 cut(s) 88, 188
VpaK11BI GGWCC 1 cut(s) 326
XceI RCATGY 1 cut(s) 395
XspI CTAG 1 cut(s) 17
ZrmI AGTACT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.