FvH4_3g25850

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012735
Physical Location & Seq
Forward (+)
18698507 .. 18700826
2320 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25850.t1

Sequence Viewer

Length: 1152 bp
ATGATTAAACACCAACTGAATTTCGAAACCCTCCAAAAAATAAAGAGTTCTAGGGAGTTCAGGCCCGAGTGTTGTTCCCCAACAAAGAGAAAGAAGAGCATAGAAGTAATGGCACCCCAAGTTAGGTTGCCAGGTGTTGATGATAAATTGCAGAAGCTTCTGGATACTAACATGGATGAAGCTCCGGCCAGAAGGAGAGTTCGTGAAGCTTTTAAGGACATTCAGCTTAAAATTGATCATATATTATTCAAGACACCAACTGATGGACTTAAAATGAAAGAGTCATATGAAGTGAACTCCAGGGGATTGGAGATCTTCTCCAAGAGTTGGCTTCCAGAGACATCTCAGCCCAAAGCAATACTTTGTTACTGTCATGGTTATGGGGATACTTGCACGTTTTTCTTTGAAGGAATCGCAAGGAAGTTGGCATCATTTGGCTATGGAGTTTTTGCAATGGACTACCCTGGATTTGGTCTTTCAGAAGGTCTGCATTGTTATATTCCTAGCTTTGAAAGGCTCGTAGATGATGTCATTGAGCATTTTTCTAAAGTCAAAACGCAACCTGAGTTCAGTACTCTCCCAAGCTTTCTTTTCGGGCAGTCCATGGGCGGAGCTGTAGCTCTTAAGATTCACCTAAAACAACCTAGTGGATGGAATGGTGCCATCCTAGTTGCACCTATGTGCAGAATTGCAGATGACATGGCCCCGCCATGGCTACTAGCACAAGTCCTAATTGGTGTTGCGAAATTCCTTCCAAAGCAGAAGTTGGTTCCACAAAAGAATCTGGCTGAGGCAGCCTTTAAAGACTTGAAGAAACGGGAACTGACGGCCTACAATGTTATTGCTTACAGGGATAACCCACGCCTGCAGACTGCTGTAGAAATGCTCCATACAACCCAAGAGATAGAAAGCCGATTAGAAGAAGTCTCTCTTCCATTATTAATCCTTCATGGAGAAGATGATATTGTGACTGATCCATCTGTAAGTAAAGCGCTCTACGAGAAAGCAAGCAGTTCTAACAAGAAGCTAATCCTGTACGAGGATGCATATCATTCCCTTCTGGAGGGTGAGCCGGATGAAATGATAGTTCAAGTTTTCAATGACATCATATCATGGCTCAATGAACACAGCACAAAGTATACAGCCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

384

Amino Acids

43.41

Weight (kDa)

6.57

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 116 - 358 1.4e-63 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 121 - 357 1.3e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 124 - 361 5.3e-14 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 112, 659
AccB7I CCANNNNNTGG 2 cut(s) 263, 327
AccI GTMKAC 1 cut(s) 1139
AciI CCGC 2 cut(s) 609, 707
AclWI GGATC 1 cut(s) 968
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 2 cut(s) 19, 746
AfaI GTAC 2 cut(s) 574, 1037
AfeI AGCGCT 1 cut(s) 993
AfiI CCNNNNNNNGG 7 cut(s) 123, 263, 327, 470, 711, 1039, 1063
AflII CTTAAG 1 cut(s) 623
AgsI TTSAA 6 cut(s) 250, 407, 512, 811, 1091, 1099
AjnI CCWGG 3 cut(s) 130, 299, 463
AleI CACNNNNGTG 1 cut(s) 679
AluBI AGCT 9 cut(s) 157, 182, 209, 226, 507, 585, 614, 620, 1027
AluI AGCT 9 cut(s) 157, 182, 209, 226, 507, 585, 614, 620, 1027
Alw26I GTCTC 2 cut(s) 332, 931
AlwI GGATC 1 cut(s) 968
Ama87I CYCGRG 1 cut(s) 65
Aor51HI AGCGCT 1 cut(s) 993
AoxI GGCC 4 cut(s) 62, 186, 702, 828
ApeKI GCWGC 1 cut(s) 794
ApoI RAATTY 2 cut(s) 19, 746
AseI ATTAAT 1 cut(s) 941
AspLEI GCGC 1 cut(s) 994
AspS9I GGNCC 2 cut(s) 63, 703
AsuHPI GGTGA 2 cut(s) 623, 1079
AsuII TTCGAA 1 cut(s) 24
AvaI CYCGRG 1 cut(s) 65
BanI GGYRCC 2 cut(s) 112, 659
BbvCI CCTCAGC 1 cut(s) 789
BbvI GCAGC 1 cut(s) 806
BccI CCATC 4 cut(s) 257, 645, 671, 985
BceAI ACGGC 1 cut(s) 843
BciT130I CCWGG 3 cut(s) 132, 301, 465
BciVI GTATCC 2 cut(s) 157, 379
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 2 cut(s) 332, 931
BfaI CTAG 5 cut(s) 51, 504, 645, 668, 719
BfmI CTRYAG 3 cut(s) 615, 866, 876
BfoI RGCGCY 1 cut(s) 995
BfrI CTTAAG 1 cut(s) 623
BfuI GTATCC 2 cut(s) 157, 379
BglII AGATCT 1 cut(s) 312
BisI GCNGC 1 cut(s) 795
BlsI GCNGC 1 cut(s) 796
BmcAI AGTACT 1 cut(s) 574
Bme1390I CCNGG 3 cut(s) 132, 301, 465
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 2 cut(s) 63, 703
BmiI GGNNCC 4 cut(s) 114, 661, 705, 771
BmrFI CCNGG 3 cut(s) 132, 301, 465
BmsI GCATC 2 cut(s) 437, 1033
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 2 cut(s) 283, 1082
Bpu10I CCTNAGC 1 cut(s) 789
Bpu14I TTCGAA 1 cut(s) 24
BsaBI GATNNNNATC 1 cut(s) 1047
BsaJI CCNNGG 4 cut(s) 300, 463, 603, 710
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bsc4I CCNNNNNNNGG 7 cut(s) 123, 263, 327, 470, 711, 1039, 1063
Bse1I ACTGG 1 cut(s) 1146
Bse3DI GCAATG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 1047
BseBI CCWGG 3 cut(s) 132, 301, 465
BseDI CCNNGG 4 cut(s) 300, 463, 603, 710
BseGI GGATG 5 cut(s) 181, 656, 663, 1048, 1081
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 7 cut(s) 123, 263, 327, 470, 711, 1039, 1063
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 3 cut(s) 359, 555, 780
BseNI ACTGG 1 cut(s) 1146
BseXI GCAGC 1 cut(s) 806
BsgI GTGCAG 1 cut(s) 703
BshFI GGCC 4 cut(s) 64, 188, 704, 830
BshNI GGYRCC 2 cut(s) 112, 659
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 2 cut(s) 185, 1073
BslI CCNNNNNNNGG 7 cut(s) 123, 263, 327, 470, 711, 1039, 1063
BsmAI GTCTC 2 cut(s) 332, 931
BsnI GGCC 4 cut(s) 64, 188, 704, 830
BsoBI CYCGRG 1 cut(s) 65
Bsp119I TTCGAA 1 cut(s) 24
Bsp143I GATC 3 cut(s) 235, 312, 973
Bsp19I CCATGG 2 cut(s) 603, 710
BspACI CCGC 2 cut(s) 609, 707
BspANI GGCC 4 cut(s) 64, 188, 704, 830
BspCNI CTCAG 3 cut(s) 358, 556, 781
BspLI GGNNCC 4 cut(s) 114, 661, 705, 771
BspMAI CTGCAG 1 cut(s) 870
BspPI GGATC 1 cut(s) 968
BspQI GCTCTTC 1 cut(s) 89
BspT104I TTCGAA 1 cut(s) 24
BspT107I GGYRCC 2 cut(s) 112, 659
BspTI CTTAAG 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 459
BsrI ACTGG 1 cut(s) 1146
BssECI CCNNGG 4 cut(s) 300, 463, 603, 710
BssMI GATC 3 cut(s) 235, 312, 973
BssNAI GTATAC 1 cut(s) 1140
BssT1I CCWWGG 2 cut(s) 603, 710
Bst1107I GTATAC 1 cut(s) 1140
Bst2UI CCWGG 3 cut(s) 132, 301, 465
Bst4CI ACNGT 1 cut(s) 371
Bst6I CTCTTC 2 cut(s) 89, 936
BstAFI CTTAAG 1 cut(s) 623
BstBI TTCGAA 1 cut(s) 24
BstC8I GCNNGC 2 cut(s) 866, 1009
BstDEI CTNAG 3 cut(s) 345, 564, 789
BstDSI CCRYGG 2 cut(s) 603, 710
BstENI CCTNNNNNAGG 2 cut(s) 1037, 1061
BstF5I GGATG 5 cut(s) 181, 656, 663, 1048, 1081
BstH2I RGCGCY 1 cut(s) 995
BstHHI GCGC 1 cut(s) 994
BstKTI GATC 3 cut(s) 238, 315, 976
BstMAI GTCTC 2 cut(s) 332, 931
BstMBI GATC 3 cut(s) 235, 312, 973
BstMWI GCNNNNNNNGC 1 cut(s) 794
BstNI CCWGG 3 cut(s) 132, 301, 465
BstSCI CCNGG 3 cut(s) 130, 299, 463
BstSFI CTRYAG 3 cut(s) 615, 866, 876
BstV1I GCAGC 1 cut(s) 806
BstX2I RGATCY 1 cut(s) 312
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 1 cut(s) 312
BstZ17I GTATAC 1 cut(s) 1140
BsuI GTATCC 2 cut(s) 157, 379
BsuRI GGCC 4 cut(s) 64, 188, 704, 830
BtgI CCRYGG 2 cut(s) 603, 710
BtsCI GGATG 5 cut(s) 181, 656, 663, 1048, 1081
Cac8I GCNNGC 2 cut(s) 866, 1009
CfoI GCGC 1 cut(s) 994
Cfr13I GGNCC 2 cut(s) 63, 703
Csp6I GTAC 2 cut(s) 573, 1036
CviAII CATG 7 cut(s) 172, 374, 604, 700, 711, 950, 1113
CviQI GTAC 2 cut(s) 573, 1036
DdeI CTNAG 3 cut(s) 345, 564, 789
DpnI GATC 3 cut(s) 237, 314, 975
DpnII GATC 3 cut(s) 235, 312, 973
DraI TTTAAA 1 cut(s) 802
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 2 cut(s) 89, 936
EarI CTCTTC 2 cut(s) 89, 936
EciI GGCGGA 1 cut(s) 624
Eco130I CCWWGG 2 cut(s) 603, 710
Eco47III AGCGCT 1 cut(s) 993
Eco88I CYCGRG 1 cut(s) 65
EcoNI CCTNNNNNAGG 2 cut(s) 1037, 1061
EcoRII CCWGG 3 cut(s) 130, 299, 463
EcoT14I CCWWGG 2 cut(s) 603, 710
EcoT22I ATGCAT 1 cut(s) 1048
ErhI CCWWGG 2 cut(s) 603, 710
FaeI CATG 7 cut(s) 175, 377, 607, 703, 714, 953, 1116
FalI AAGNNNNNCTT 4 cut(s) 345, 377, 915, 947
FatI CATG 7 cut(s) 171, 373, 603, 699, 710, 949, 1112
FauI CCCGC 1 cut(s) 714
FauNDI CATATG 1 cut(s) 286
FbaI TGATCA 1 cut(s) 235
FblI GTMKAC 1 cut(s) 1139
Fnu4HI GCNGC 1 cut(s) 795
FokI GGATG 5 cut(s) 188, 650, 663, 1055, 1088
Fsp4HI GCNGC 1 cut(s) 795
FspBI CTAG 5 cut(s) 51, 504, 645, 668, 719
GlaI GCGC 1 cut(s) 993
GluI GCNGC 1 cut(s) 795
GsuI CTGGAG 2 cut(s) 283, 1082
HaeII RGCGCY 1 cut(s) 995
HaeIII GGCC 4 cut(s) 64, 188, 704, 830
HapII CCGG 2 cut(s) 185, 1073
HhaI GCGC 1 cut(s) 994
Hin1II CATG 7 cut(s) 175, 377, 607, 703, 714, 953, 1116
Hin6I GCGC 1 cut(s) 992
HinP1I GCGC 1 cut(s) 992
HindIII AAGCTT 3 cut(s) 155, 207, 583
HinfI GANTC 4 cut(s) 281, 411, 628, 781
HpaII CCGG 2 cut(s) 185, 1073
HphI GGTGA 2 cut(s) 623, 1079
Hpy166II GTNNAC 2 cut(s) 295, 1140
Hpy188I TCNGA 1 cut(s) 481
Hpy188III TCNNGA 5 cut(s) 161, 203, 250, 335, 1061
Hpy8I GTNNAC 2 cut(s) 295, 1140
HpyAV CCTTC 6 cut(s) 186, 401, 476, 761, 956, 1067
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4IV ACGT 1 cut(s) 395
HpyCH4V TGCA 9 cut(s) 151, 393, 452, 490, 674, 684, 692, 868, 1046
HpyF10VI GCNNNNNNNGC 1 cut(s) 794
HpyF3I CTNAG 3 cut(s) 345, 564, 789
HpySE526I ACGT 1 cut(s) 395
Hsp92II CATG 7 cut(s) 175, 377, 607, 703, 714, 953, 1116
HspAI GCGC 1 cut(s) 992
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 3 cut(s) 235, 312, 973
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 3 cut(s) 187, 611, 891
Lsp1109I GCAGC 1 cut(s) 806
LweI GCATC 2 cut(s) 437, 1033
MaeI CTAG 5 cut(s) 51, 504, 645, 668, 719
MaeII ACGT 1 cut(s) 395
MaeIII GTNAC 2 cut(s) 365, 967
MalI GATC 3 cut(s) 237, 314, 975
MboI GATC 3 cut(s) 235, 312, 973
MboII GAAGA 6 cut(s) 106, 307, 823, 923, 932, 968
MflI RGATCY 1 cut(s) 312
MluCI AATT 6 cut(s) 19, 146, 231, 687, 732, 746
MlyI GAGTC 1 cut(s) 290
MnlI CCTC 4 cut(s) 41, 784, 1033, 1057
Mph1103I ATGCAT 1 cut(s) 1048
MseI TTAA 7 cut(s) 6, 213, 228, 270, 624, 801, 941
MslI CAYNNNNRTG 2 cut(s) 378, 679
MspCI CTTAAG 1 cut(s) 623
MspI CCGG 2 cut(s) 185, 1073
MspR9I CCNGG 3 cut(s) 132, 301, 465
MvaI CCWGG 3 cut(s) 132, 301, 465
MwoI GCNNNNNNNGC 1 cut(s) 794
NcoI CCATGG 2 cut(s) 603, 710
NdeI CATATG 1 cut(s) 286
NdeII GATC 3 cut(s) 235, 312, 973
NlaIII CATG 7 cut(s) 175, 377, 607, 703, 714, 953, 1116
NlaIV GGNNCC 4 cut(s) 114, 661, 705, 771
NmuCI GTSAC 1 cut(s) 967
NsiI ATGCAT 1 cut(s) 1048
NspV TTCGAA 1 cut(s) 24
OliI CACNNNNGTG 1 cut(s) 679
PciSI GCTCTTC 1 cut(s) 89
PfeI GAWTC 3 cut(s) 411, 628, 781
PflMI CCANNNNNTGG 2 cut(s) 263, 327
PkrI GCNGC 1 cut(s) 796
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
PshBI ATTAAT 1 cut(s) 941
Psp6I CCWGG 3 cut(s) 130, 299, 463
PspGI CCWGG 3 cut(s) 130, 299, 463
PspN4I GGNNCC 4 cut(s) 114, 661, 705, 771
PspPI GGNCC 2 cut(s) 63, 703
PstI CTGCAG 1 cut(s) 870
PsuI RGATCY 1 cut(s) 312
RsaI GTAC 2 cut(s) 574, 1037
RsaNI GTAC 2 cut(s) 573, 1036
RseI CAYNNNNRTG 2 cut(s) 378, 679
SapI GCTCTTC 1 cut(s) 89
SaqAI TTAA 7 cut(s) 6, 213, 228, 270, 624, 801, 941
SatI GCNGC 1 cut(s) 795
Sau3AI GATC 3 cut(s) 235, 312, 973
Sau96I GGNCC 2 cut(s) 63, 703
ScaI AGTACT 1 cut(s) 574
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 3 cut(s) 132, 301, 465
SfaNI GCATC 2 cut(s) 437, 1033
SfcI CTRYAG 3 cut(s) 615, 866, 876
SfuI TTCGAA 1 cut(s) 24
SmiMI CAYNNNNRTG 2 cut(s) 378, 679
SmlI CTYRAG 1 cut(s) 623
SmoI CTYRAG 1 cut(s) 623
Sse9I AATT 6 cut(s) 19, 146, 231, 687, 732, 746
SsiI CCGC 2 cut(s) 609, 707
SspMI CTAG 5 cut(s) 51, 504, 645, 668, 719
StyD4I CCNGG 3 cut(s) 130, 299, 463
StyI CCWWGG 2 cut(s) 603, 710
TaaI ACNGT 1 cut(s) 371
TaiI ACGT 1 cut(s) 398
TaqI TCGA 1 cut(s) 24
TasI AATT 6 cut(s) 19, 146, 231, 687, 732, 746
TatI WGTACW 1 cut(s) 572
TfiI GAWTC 3 cut(s) 411, 628, 781
Tru1I TTAA 7 cut(s) 6, 213, 228, 270, 624, 801, 941
Tru9I TTAA 7 cut(s) 6, 213, 228, 270, 624, 801, 941
TseFI GTSAC 1 cut(s) 967
TseI GCWGC 1 cut(s) 794
Tsp45I GTSAC 1 cut(s) 967
TspDTI ATGAA 6 cut(s) 192, 290, 303, 938, 1092, 1137
Van91I CCANNNNNTGG 2 cut(s) 263, 327
Vha464I CTTAAG 1 cut(s) 623
VspI ATTAAT 1 cut(s) 941
XagI CCTNNNNNAGG 2 cut(s) 1037, 1061
XapI RAATTY 2 cut(s) 19, 746
XmiI GTMKAC 1 cut(s) 1139
XspI CTAG 5 cut(s) 51, 504, 645, 668, 719
ZrmI AGTACT 1 cut(s) 574
Zsp2I ATGCAT 1 cut(s) 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.