Rmu_co8345237.1_g000001

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8345237.1
OG0012735
Physical Location & Seq
Forward (+)
351 .. 1022
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8345237.1_g000001.1.cds

Sequence Viewer

Length: 672 bp
atggcaccccaagtgaggttgcaaggtgttgatgataaattgcagaagcttctggatactaacatggatgaagctccggccagaaggagagctcgtgaagcttttaaggacattcagcttaatattgatcatatgttattcaagacaccaactgatggactgaatataaaagagtcatatgaagtgaactccaggggagtggagatcttctccaagagttggcttccagagacatctcagcccaaagcactactgtgttactgtcatggttacggagatacttgcacatttttctttgaaggaattgcaaggaagttggcatcatatggctatggagtttttgcaatggactaccctggatttggtctttcggaaggtcttcattgctatattcctagctttgaaaggctcgtggatgatgtcattgagcatttctctaaagtcaaagagcaacctgagttcaatgctctcccaagctttctcttcgggcagtccatgggcggagctgtagctcttaagattcacctaaaacaacctagtggatggaatggtgctatcctagttgcacctatgtgcagaattgcagatgatatttccccaccgtggctgctagcacaagtcctaattggtgttgctaaattccttccaaagcagaagttggttccacaaaag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

224

Amino Acids

25.1

Weight (kDa)

6.95

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 2 cut(s) 155, 219
AciI CCGC 1 cut(s) 501
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 1 cut(s) 638
AfiI CCNNNNNNNGG 5 cut(s) 15, 155, 219, 362, 603
AflII CTTAAG 1 cut(s) 515
AgsI TTSAA 4 cut(s) 142, 299, 404, 463
AjnI CCWGG 2 cut(s) 191, 355
AleI CACNNNNGTG 2 cut(s) 253, 571
AluBI AGCT 9 cut(s) 49, 74, 92, 101, 118, 399, 477, 506, 512
AluI AGCT 9 cut(s) 49, 74, 92, 101, 118, 399, 477, 506, 512
Alw21I GWGCWC 1 cut(s) 94
Alw26I GTCTC 1 cut(s) 224
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 1 cut(s) 607
ApoI RAATTY 1 cut(s) 638
Asp700I GAANNNNTTC 1 cut(s) 378
AsuHPI GGTGA 1 cut(s) 515
AsuNHI GCTAGC 1 cut(s) 610
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 94
BauI CACGAG 2 cut(s) 93, 410
BbsI GAAGAC 1 cut(s) 371
Bbv12I GWGCWC 1 cut(s) 94
BbvI GCAGC 1 cut(s) 594
BccI CCATC 2 cut(s) 149, 537
BciT130I CCWGG 2 cut(s) 193, 357
BciVI GTATCC 1 cut(s) 49
BclI TGATCA 1 cut(s) 127
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 4 cut(s) 396, 537, 560, 611
BfmI CTRYAG 1 cut(s) 507
BfrI CTTAAG 1 cut(s) 515
BfuI GTATCC 1 cut(s) 49
BglII AGATCT 1 cut(s) 204
BisI GCNGC 1 cut(s) 608
BlsI GCNGC 1 cut(s) 609
Bme1390I CCNGG 2 cut(s) 193, 357
BmiI GGNNCC 2 cut(s) 6, 663
BmrFI CCNGG 2 cut(s) 193, 357
BmsI GCATC 1 cut(s) 329
BmtI GCTAGC 1 cut(s) 614
BpiI GAAGAC 1 cut(s) 371
BplI GAGNNNNNCTC 4 cut(s) 194, 226, 419, 451
BpmI CTGGAG 1 cut(s) 175
BsaJI CCNNGG 4 cut(s) 192, 355, 495, 602
BsaXI ACNNNNNCTCC 2 cut(s) 79, 109
Bsc4I CCNNNNNNNGG 5 cut(s) 15, 155, 219, 362, 603
Bse3DI GCAATG 2 cut(s) 351, 382
BseBI CCWGG 2 cut(s) 193, 357
BseDI CCNNGG 4 cut(s) 192, 355, 495, 602
BseGI GGATG 3 cut(s) 73, 421, 548
BseLI CCNNNNNNNGG 5 cut(s) 15, 155, 219, 362, 603
BseMI GCAATG 2 cut(s) 351, 382
BseMII CTCAG 2 cut(s) 251, 447
BseXI GCAGC 1 cut(s) 594
BsgI GTGCAG 1 cut(s) 595
BshFI GGCC 1 cut(s) 80
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 94
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 5 cut(s) 15, 155, 219, 362, 603
BsmAI GTCTC 1 cut(s) 224
BsnI GGCC 1 cut(s) 80
Bsp1286I GDGCHC 1 cut(s) 94
Bsp143I GATC 2 cut(s) 127, 204
Bsp19I CCATGG 1 cut(s) 495
BspACI CCGC 1 cut(s) 501
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 2 cut(s) 250, 448
BspLI GGNNCC 2 cut(s) 6, 663
BspOI GCTAGC 1 cut(s) 614
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 515
BsrDI GCAATG 2 cut(s) 351, 382
BssECI CCNNGG 4 cut(s) 192, 355, 495, 602
BssMI GATC 2 cut(s) 127, 204
BssSI CACGAG 2 cut(s) 93, 410
BssT1I CCWWGG 1 cut(s) 495
Bst2BI CACGAG 2 cut(s) 93, 410
Bst2UI CCWGG 2 cut(s) 193, 357
Bst4CI ACNGT 3 cut(s) 255, 263, 603
Bst6I CTCTTC 1 cut(s) 488
BstAFI CTTAAG 1 cut(s) 515
BstC8I GCNNGC 1 cut(s) 612
BstDEI CTNAG 2 cut(s) 237, 456
BstDSI CCRYGG 2 cut(s) 495, 602
BstF5I GGATG 3 cut(s) 73, 421, 548
BstKTI GATC 2 cut(s) 130, 207
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 2 cut(s) 127, 204
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNI CCWGG 2 cut(s) 193, 357
BstSCI CCNGG 2 cut(s) 191, 355
BstSFI CTRYAG 1 cut(s) 507
BstV1I GCAGC 1 cut(s) 594
BstV2I GAAGAC 1 cut(s) 371
BstX2I RGATCY 1 cut(s) 204
BstXI CCANNNNNNTGG 1 cut(s) 199
BstYI RGATCY 1 cut(s) 204
BsuI GTATCC 1 cut(s) 49
BsuRI GGCC 1 cut(s) 80
BtgI CCRYGG 2 cut(s) 495, 602
BtsCI GGATG 3 cut(s) 73, 421, 548
Cac8I GCNNGC 1 cut(s) 612
CviAII CATG 3 cut(s) 64, 266, 496
DdeI CTNAG 2 cut(s) 237, 456
DpnI GATC 2 cut(s) 129, 206
DpnII GATC 2 cut(s) 127, 204
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 488
EarI CTCTTC 1 cut(s) 488
EciI GGCGGA 1 cut(s) 516
Ecl136II GAGCTC 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 94
Eco53kI GAGCTC 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 92
EcoRII CCWGG 2 cut(s) 191, 355
EcoT14I CCWWGG 1 cut(s) 495
EcoT38I GRGCYC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 3 cut(s) 67, 269, 499
FatI CATG 3 cut(s) 63, 265, 495
FauNDI CATATG 3 cut(s) 132, 178, 325
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 1 cut(s) 608
FokI GGATG 3 cut(s) 80, 428, 555
FriOI GRGCYC 1 cut(s) 94
Fsp4HI GCNGC 1 cut(s) 608
FspBI CTAG 4 cut(s) 396, 537, 560, 611
GluI GCNGC 1 cut(s) 608
GsuI CTGGAG 1 cut(s) 175
HaeIII GGCC 1 cut(s) 80
HapII CCGG 1 cut(s) 77
Hin1II CATG 3 cut(s) 67, 269, 499
HindIII AAGCTT 3 cut(s) 47, 99, 475
HinfI GANTC 2 cut(s) 173, 520
HpaII CCGG 1 cut(s) 77
HphI GGTGA 1 cut(s) 515
Hpy166II GTNNAC 1 cut(s) 187
Hpy188I TCNGA 1 cut(s) 373
Hpy188III TCNNGA 4 cut(s) 53, 95, 142, 227
Hpy8I GTNNAC 1 cut(s) 187
HpyAV CCTTC 4 cut(s) 78, 293, 368, 653
HpyCH4III ACNGT 3 cut(s) 255, 263, 603
HpyCH4V TGCA 8 cut(s) 22, 43, 285, 308, 344, 566, 576, 584
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 2 cut(s) 237, 456
Hsp92II CATG 3 cut(s) 67, 269, 499
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 2 cut(s) 127, 204
LmnI GCTCC 2 cut(s) 79, 503
LpnPI CCDG 9 cut(s) 38, 90, 94, 178, 205, 240, 342, 369, 468
Lsp1109I GCAGC 1 cut(s) 594
LweI GCATC 1 cut(s) 329
MaeI CTAG 4 cut(s) 396, 537, 560, 611
MaeIII GTNAC 2 cut(s) 257, 269
MalI GATC 2 cut(s) 129, 206
MboI GATC 2 cut(s) 127, 204
MboII GAAGA 3 cut(s) 199, 371, 475
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 94
MluCI AATT 5 cut(s) 38, 303, 579, 624, 638
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 1 cut(s) 9
MroXI GAANNNNTTC 1 cut(s) 378
MseI TTAA 3 cut(s) 105, 120, 516
MslI CAYNNNNRTG 2 cut(s) 253, 571
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 2 cut(s) 193, 357
MvaI CCWGG 2 cut(s) 193, 357
MwoI GCNNNNNNNGC 1 cut(s) 98
NcoI CCATGG 1 cut(s) 495
NdeI CATATG 3 cut(s) 132, 178, 325
NdeII GATC 2 cut(s) 127, 204
NheI GCTAGC 1 cut(s) 610
NlaIII CATG 3 cut(s) 67, 269, 499
NlaIV GGNNCC 2 cut(s) 6, 663
OliI CACNNNNGTG 2 cut(s) 253, 571
PdmI GAANNNNTTC 1 cut(s) 378
PfeI GAWTC 1 cut(s) 520
PflMI CCANNNNNTGG 2 cut(s) 155, 219
PkrI GCNGC 1 cut(s) 609
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 94
Psp6I CCWGG 2 cut(s) 191, 355
PspGI CCWGG 2 cut(s) 191, 355
PspN4I GGNNCC 2 cut(s) 6, 663
PsuI RGATCY 1 cut(s) 204
RseI CAYNNNNRTG 2 cut(s) 253, 571
SacI GAGCTC 1 cut(s) 94
SaqAI TTAA 3 cut(s) 105, 120, 516
SatI GCNGC 1 cut(s) 608
Sau3AI GATC 2 cut(s) 127, 204
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 193, 357
SduI GDGCHC 1 cut(s) 94
SfaNI GCATC 1 cut(s) 329
SfcI CTRYAG 1 cut(s) 507
SmiMI CAYNNNNRTG 2 cut(s) 253, 571
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 5 cut(s) 38, 303, 579, 624, 638
SsiI CCGC 1 cut(s) 501
SspI AATATT 1 cut(s) 124
SspMI CTAG 4 cut(s) 396, 537, 560, 611
SstI GAGCTC 1 cut(s) 94
StyD4I CCNGG 2 cut(s) 191, 355
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 3 cut(s) 255, 263, 603
TasI AATT 5 cut(s) 38, 303, 579, 624, 638
TfiI GAWTC 1 cut(s) 520
Tru1I TTAA 3 cut(s) 105, 120, 516
Tru9I TTAA 3 cut(s) 105, 120, 516
TseI GCWGC 1 cut(s) 607
TspDTI ATGAA 3 cut(s) 84, 195, 371
TspGWI ACGGA 1 cut(s) 288
Van91I CCANNNNNTGG 2 cut(s) 155, 219
Vha464I CTTAAG 1 cut(s) 515
XapI RAATTY 1 cut(s) 638
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 4 cut(s) 396, 537, 560, 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.