Rh5AG312900

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012735
Physical Location & Seq
Forward (+)
45251565 .. 45256636
5072 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG312900.1

Sequence Viewer

Length: 1365 bp
ATGTACATGTATTCTCTTTTTCTGCAGCTTTCAAGTCATGAGCTGCTTAGCCAAAAGAGGTCGCAGGAATGGAAACCCAGAAGCAGTTTCAAATCAACCTCGGTAATCTCATGCCCTTTGACCCTCATCACCAATTCCCCTCCACTCCATCCTCCAGAAAAGACTTGCTGCCTAATACTTGTTTCTGATTACAATTGGCATTCTGTTATTGTCATGATTAAACACCAACTGAATTTCAAAATCCTCCAAAGAATAAAGAGTTCTCGGAAGTTCAGGCCCGAGTGTTGTTCCCCAACAAAGAGAAAGAAGAGCATAGAAGTAATGGCACCCCAAGTGAGGTTGCAAGGTGTTGATGATAAATTGCAGAAGCTTCTGGATACTAACATGGATGAAGCTCCGGCCAGAAGGAGAGCTCGTGAAGCTTTTAAGGACGTTCAGCTTAATATTGATCATATGTTATTCAAGACACCAACTGATGGACTGAATATAAAAGAGTCATATGAAGTGAACTCCAGGGGATTGGAGATCTTCTCCAAGAGTTGGCTTCCAGAGACATCTCAGCCCAAAGCACTACTGTGTTACTGTCATGGTTACGGAGATACTTGCACATTTTTCTTTGAAGGAATTGCAAGGAAGTTGGCATCATATGGCTATGGAGTTTTTGCAATGGACTACCCTGGATTTGGTCTTTCGGAAGGTCTTCATTGCTACATTCCTAGCTTTGAAAGGCTCGTGGATGATGTCATTGAGCATTTCTCTAAAGTCAAAGAGCAACCTGAGTTCAATGTTCTCCCAAGCTTTCTCTTCGGGCAGTCCATGGGCGGAGCTGTAGCTCTTAAGATTCACCTAAAACAACCTAGTGGATGGAATGGTGCTATCCTAGTTGCACCTATGTGCAGAATTGCAGATGACATTTCCCCACCGTGGCTGCTAGCACAAGTCCTAATTGGTGTTGCTAAATTCCTTCCAAAGCAGAAGTTGGTTCCACAAAAGAATCTGGCTGAGGCAGCCTTTAAAGACTTGAAGAAACGGGAAATGACGGCCTATAATGTTGTTGCTTACAAGGATAAGCCACGCCTGCAGACTGCTGTAGAAATGCTCCGGACAACTCAAGAGATAGAAAGTCGATTAGAAGAAGTCTCTCTTCCATTATTAATCCTTCACGGAGAAGCTGATACTGTGACCGATCCATCTGTGAGTAGGGCGCTTTATGAGAAAGCAAGCAGTTCCGACAAGAAGCTAATCCTTTACGAGGATGCATATCATTCCCTTCTGGAAGGTGAGCCGGATGAAATGATAGTTCAAGTTTTCAATGACATCATATCATGGCTCAACGAACACAGCACAAAATTTACAGCCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

454

Amino Acids

51.69

Weight (kDa)

8.37

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 187 - 429 1.8e-64 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 191 - 428 3.7e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 195 - 432 1.4e-13 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 325
AccB7I CCANNNNNTGG 2 cut(s) 476, 540
AccIII TCCGGA 1 cut(s) 1101
AciI CCGC 1 cut(s) 822
AclWI GGATC 1 cut(s) 1181
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 3 cut(s) 232, 959, 1349
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 6 cut(s) 336, 476, 540, 683, 924, 1252
AflII CTTAAG 1 cut(s) 836
AflIII ACRYGT 1 cut(s) 6
AjnI CCWGG 2 cut(s) 512, 676
AleI CACNNNNGTG 2 cut(s) 574, 892
Alw21I GWGCWC 1 cut(s) 415
Alw26I GTCTC 2 cut(s) 545, 1144
AlwI GGATC 1 cut(s) 1181
Ama87I CYCGRG 1 cut(s) 278
Aor13HI TCCGGA 1 cut(s) 1101
AoxI GGCC 3 cut(s) 275, 399, 1041
ApeKI GCWGC 5 cut(s) 25, 43, 168, 928, 1007
ApoI RAATTY 3 cut(s) 232, 959, 1349
AseI ATTAAT 1 cut(s) 1154
Asp700I GAANNNNTTC 1 cut(s) 699
AspLEI GCGC 1 cut(s) 1207
AspS9I GGNCC 1 cut(s) 276
AsuHPI GGTGA 3 cut(s) 121, 836, 1292
AsuNHI GCTAGC 1 cut(s) 931
AvaI CYCGRG 1 cut(s) 278
BanI GGYRCC 1 cut(s) 325
BanII GRGCYC 1 cut(s) 415
BauI CACGAG 2 cut(s) 414, 731
BbsI GAAGAC 1 cut(s) 692
Bbv12I GWGCWC 1 cut(s) 415
BbvCI CCTCAGC 1 cut(s) 1002
BbvI GCAGC 5 cut(s) 30, 37, 155, 915, 1019
BccI CCATC 4 cut(s) 156, 470, 858, 1198
BceAI ACGGC 1 cut(s) 1056
BciT130I CCWGG 2 cut(s) 514, 678
BciVI GTATCC 1 cut(s) 370
BclI TGATCA 1 cut(s) 448
BcoDI GTCTC 2 cut(s) 545, 1144
BfaI CTAG 4 cut(s) 717, 858, 881, 932
BfmI CTRYAG 4 cut(s) 23, 828, 1079, 1089
BfoI RGCGCY 1 cut(s) 1208
BfrI CTTAAG 1 cut(s) 836
BfuI GTATCC 1 cut(s) 370
BglII AGATCT 1 cut(s) 525
BisI GCNGC 5 cut(s) 26, 44, 169, 929, 1008
BlpI GCTNAGC 1 cut(s) 47
BlsI GCNGC 5 cut(s) 27, 45, 170, 930, 1009
Bme1390I CCNGG 2 cut(s) 514, 678
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 276
BmiI GGNNCC 2 cut(s) 327, 984
BmrFI CCNGG 2 cut(s) 514, 678
BmsI GCATC 2 cut(s) 650, 1246
BmtI GCTAGC 1 cut(s) 935
BpiI GAAGAC 1 cut(s) 692
BplI GAGNNNNNCTC 4 cut(s) 515, 547, 740, 772
BpmI CTGGAG 2 cut(s) 138, 496
Bpu10I CCTNAGC 1 cut(s) 1002
Bpu1102I GCTNAGC 1 cut(s) 47
BpuEI CTTGAG 1 cut(s) 1095
BsaBI GATNNNNATC 1 cut(s) 1260
BsaJI CCNNGG 5 cut(s) 99, 513, 676, 816, 923
BsaWI WCCGGW 1 cut(s) 1101
BsaXI ACNNNNNCTCC 2 cut(s) 400, 430
Bsc4I CCNNNNNNNGG 6 cut(s) 336, 476, 540, 683, 924, 1252
Bse3DI GCAATG 2 cut(s) 672, 703
Bse8I GATNNNNATC 1 cut(s) 1260
BseAI TCCGGA 1 cut(s) 1101
BseBI CCWGG 2 cut(s) 514, 678
BseDI CCNNGG 5 cut(s) 99, 513, 676, 816, 923
BseGI GGATG 6 cut(s) 148, 394, 742, 869, 1261, 1294
BseJI GATNNNNATC 1 cut(s) 1260
BseLI CCNNNNNNNGG 6 cut(s) 336, 476, 540, 683, 924, 1252
BseMI GCAATG 2 cut(s) 672, 703
BseMII CTCAG 3 cut(s) 572, 768, 993
BseXI GCAGC 5 cut(s) 30, 37, 155, 915, 1019
BsgI GTGCAG 1 cut(s) 916
BshFI GGCC 3 cut(s) 277, 401, 1043
BshNI GGYRCC 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 415
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 3 cut(s) 398, 1102, 1286
BslI CCNNNNNNNGG 6 cut(s) 336, 476, 540, 683, 924, 1252
BsmAI GTCTC 2 cut(s) 545, 1144
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 3 cut(s) 277, 401, 1043
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 415
Bsp13I TCCGGA 1 cut(s) 1101
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 448, 525, 1186
Bsp1720I GCTNAGC 1 cut(s) 47
Bsp19I CCATGG 1 cut(s) 816
BspACI CCGC 1 cut(s) 822
BspANI GGCC 3 cut(s) 277, 401, 1043
BspCNI CTCAG 3 cut(s) 571, 769, 994
BspEI TCCGGA 1 cut(s) 1101
BspHI TCATGA 2 cut(s) 37, 213
BspLI GGNNCC 2 cut(s) 327, 984
BspMAI CTGCAG 2 cut(s) 27, 1083
BspOI GCTAGC 1 cut(s) 935
BspPI GGATC 1 cut(s) 1181
BspQI GCTCTTC 1 cut(s) 302
BspT107I GGYRCC 1 cut(s) 325
BspTI CTTAAG 1 cut(s) 836
BsrDI GCAATG 2 cut(s) 672, 703
BsrGI TGTACA 1 cut(s) 3
BssECI CCNNGG 5 cut(s) 99, 513, 676, 816, 923
BssMI GATC 3 cut(s) 448, 525, 1186
BssSI CACGAG 2 cut(s) 414, 731
BssT1I CCWWGG 1 cut(s) 816
Bst2BI CACGAG 2 cut(s) 414, 731
Bst2UI CCWGG 2 cut(s) 514, 678
Bst4CI ACNGT 4 cut(s) 576, 584, 924, 1180
Bst6I CTCTTC 3 cut(s) 302, 809, 1149
BstAFI CTTAAG 1 cut(s) 836
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 3 cut(s) 933, 1079, 1222
BstDEI CTNAG 4 cut(s) 47, 558, 777, 1002
BstDSI CCRYGG 2 cut(s) 816, 923
BstENI CCTNNNNNAGG 1 cut(s) 1250
BstF5I GGATG 6 cut(s) 148, 394, 742, 869, 1261, 1294
BstH2I RGCGCY 1 cut(s) 1208
BstHHI GCGC 1 cut(s) 1207
BstKTI GATC 3 cut(s) 451, 528, 1189
BstMAI GTCTC 2 cut(s) 545, 1144
BstMBI GATC 3 cut(s) 448, 525, 1186
BstMWI GCNNNNNNNGC 3 cut(s) 419, 1007, 1078
BstNI CCWGG 2 cut(s) 514, 678
BstNSI RCATGY 1 cut(s) 10
BstSCI CCNGG 2 cut(s) 512, 676
BstSFI CTRYAG 4 cut(s) 23, 828, 1079, 1089
BstV1I GCAGC 5 cut(s) 30, 37, 155, 915, 1019
BstV2I GAAGAC 1 cut(s) 692
BstX2I RGATCY 1 cut(s) 525
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 1 cut(s) 525
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 3 cut(s) 277, 401, 1043
BtgI CCRYGG 2 cut(s) 816, 923
BtsCI GGATG 6 cut(s) 148, 394, 742, 869, 1261, 1294
Cac8I GCNNGC 3 cut(s) 933, 1079, 1222
CciI TCATGA 2 cut(s) 37, 213
CfoI GCGC 1 cut(s) 1207
Cfr13I GGNCC 1 cut(s) 276
Csp6I GTAC 1 cut(s) 4
CviAII CATG 8 cut(s) 7, 38, 111, 214, 385, 587, 817, 1326
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 4 cut(s) 47, 558, 777, 1002
DpnI GATC 3 cut(s) 450, 527, 1188
DpnII GATC 3 cut(s) 448, 525, 1186
DraI TTTAAA 1 cut(s) 1015
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 3 cut(s) 302, 809, 1149
EarI CTCTTC 3 cut(s) 302, 809, 1149
EciI GGCGGA 1 cut(s) 837
Ecl136II GAGCTC 1 cut(s) 413
Eco130I CCWWGG 1 cut(s) 816
Eco24I GRGCYC 1 cut(s) 415
Eco53kI GAGCTC 1 cut(s) 413
Eco88I CYCGRG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 413
EcoNI CCTNNNNNAGG 1 cut(s) 1250
EcoRII CCWGG 2 cut(s) 512, 676
EcoT14I CCWWGG 1 cut(s) 816
EcoT22I ATGCAT 1 cut(s) 1261
EcoT38I GRGCYC 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 816
FaeI CATG 8 cut(s) 10, 41, 114, 217, 388, 590, 820, 1329
FalI AAGNNNNNCTT 2 cut(s) 1128, 1160
FatI CATG 8 cut(s) 6, 37, 110, 213, 384, 586, 816, 1325
FauNDI CATATG 3 cut(s) 453, 499, 646
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 5 cut(s) 26, 44, 169, 929, 1008
FokI GGATG 6 cut(s) 135, 401, 749, 876, 1268, 1301
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 5 cut(s) 26, 44, 169, 929, 1008
FspBI CTAG 4 cut(s) 717, 858, 881, 932
GlaI GCGC 1 cut(s) 1206
GluI GCNGC 5 cut(s) 26, 44, 169, 929, 1008
GsuI CTGGAG 2 cut(s) 138, 496
HaeII RGCGCY 1 cut(s) 1208
HaeIII GGCC 3 cut(s) 277, 401, 1043
HapII CCGG 3 cut(s) 398, 1102, 1286
HhaI GCGC 1 cut(s) 1207
Hin1II CATG 8 cut(s) 10, 41, 114, 217, 388, 590, 820, 1329
Hin6I GCGC 1 cut(s) 1205
HinP1I GCGC 1 cut(s) 1205
HindIII AAGCTT 3 cut(s) 368, 420, 796
HinfI GANTC 3 cut(s) 494, 841, 994
HpaII CCGG 3 cut(s) 398, 1102, 1286
HphI GGTGA 3 cut(s) 121, 836, 1292
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 4 cut(s) 187, 267, 694, 1231
Hpy8I GTNNAC 1 cut(s) 508
HpyAV CCTTC 7 cut(s) 399, 614, 689, 974, 1169, 1271, 1280
HpyCH4III ACNGT 4 cut(s) 576, 584, 924, 1180
HpyCH4IV ACGT 1 cut(s) 432
HpyF10VI GCNNNNNNNGC 3 cut(s) 419, 1007, 1078
HpyF3I CTNAG 4 cut(s) 47, 558, 777, 1002
HpySE526I ACGT 1 cut(s) 432
Hsp92II CATG 8 cut(s) 10, 41, 114, 217, 388, 590, 820, 1329
HspAI GCGC 1 cut(s) 1205
Kpn2I TCCGGA 1 cut(s) 1101
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 3 cut(s) 448, 525, 1186
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 3 cut(s) 400, 824, 1104
Lsp1109I GCAGC 5 cut(s) 30, 37, 155, 915, 1019
LweI GCATC 2 cut(s) 650, 1246
MaeI CTAG 4 cut(s) 717, 858, 881, 932
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 3 cut(s) 578, 590, 1180
MalI GATC 3 cut(s) 450, 527, 1188
MboI GATC 3 cut(s) 448, 525, 1186
MboII GAAGA 7 cut(s) 319, 520, 692, 796, 1036, 1136, 1145
MfeI CAATTG 1 cut(s) 193
MflI RGATCY 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 9 cut(s) 133, 193, 232, 359, 624, 900, 945, 959, 1349
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 1 cut(s) 1254
MnlI CCTC 9 cut(s) 51, 109, 134, 150, 162, 254, 330, 997, 1246
Mph1103I ATGCAT 1 cut(s) 1261
MroI TCCGGA 1 cut(s) 1101
MroXI GAANNNNTTC 1 cut(s) 699
MseI TTAA 6 cut(s) 219, 426, 441, 837, 1014, 1154
MslI CAYNNNNRTG 2 cut(s) 574, 892
MspCI CTTAAG 1 cut(s) 836
MspI CCGG 3 cut(s) 398, 1102, 1286
MspR9I CCNGG 2 cut(s) 514, 678
MunI CAATTG 1 cut(s) 193
Mva1269I GAATGC 1 cut(s) 199
MvaI CCWGG 2 cut(s) 514, 678
MwoI GCNNNNNNNGC 3 cut(s) 419, 1007, 1078
NcoI CCATGG 1 cut(s) 816
NdeI CATATG 3 cut(s) 453, 499, 646
NdeII GATC 3 cut(s) 448, 525, 1186
NheI GCTAGC 1 cut(s) 931
NlaIII CATG 8 cut(s) 10, 41, 114, 217, 388, 590, 820, 1329
NlaIV GGNNCC 2 cut(s) 327, 984
NmuCI GTSAC 1 cut(s) 1180
NsiI ATGCAT 1 cut(s) 1261
NspI RCATGY 1 cut(s) 10
OliI CACNNNNGTG 2 cut(s) 574, 892
PagI TCATGA 2 cut(s) 37, 213
PciI ACATGT 1 cut(s) 6
PciSI GCTCTTC 1 cut(s) 302
PctI GAATGC 1 cut(s) 199
PdmI GAANNNNTTC 1 cut(s) 699
PfeI GAWTC 2 cut(s) 841, 994
PflMI CCANNNNNTGG 2 cut(s) 476, 540
PkrI GCNGC 5 cut(s) 27, 45, 170, 930, 1009
PleI GAGTC 1 cut(s) 502
PpsI GAGTC 1 cut(s) 502
PscI ACATGT 1 cut(s) 6
PshBI ATTAAT 1 cut(s) 1154
Psp124BI GAGCTC 1 cut(s) 415
Psp6I CCWGG 2 cut(s) 512, 676
PspGI CCWGG 2 cut(s) 512, 676
PspN4I GGNNCC 2 cut(s) 327, 984
PspPI GGNCC 1 cut(s) 276
PstI CTGCAG 2 cut(s) 27, 1083
PsuI RGATCY 1 cut(s) 525
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 574, 892
SacI GAGCTC 1 cut(s) 415
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 6 cut(s) 219, 426, 441, 837, 1014, 1154
SatI GCNGC 5 cut(s) 26, 44, 169, 929, 1008
Sau3AI GATC 3 cut(s) 448, 525, 1186
Sau96I GGNCC 1 cut(s) 276
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 2 cut(s) 514, 678
SduI GDGCHC 1 cut(s) 415
SfaNI GCATC 2 cut(s) 650, 1246
SfcI CTRYAG 4 cut(s) 23, 828, 1079, 1089
SmiMI CAYNNNNRTG 2 cut(s) 574, 892
SmlI CTYRAG 2 cut(s) 836, 1110
SmoI CTYRAG 2 cut(s) 836, 1110
Sse9I AATT 9 cut(s) 133, 193, 232, 359, 624, 900, 945, 959, 1349
SsiI CCGC 1 cut(s) 822
SspI AATATT 1 cut(s) 445
SspMI CTAG 4 cut(s) 717, 858, 881, 932
SstI GAGCTC 1 cut(s) 415
StyD4I CCNGG 2 cut(s) 512, 676
StyI CCWWGG 1 cut(s) 816
TaaI ACNGT 4 cut(s) 576, 584, 924, 1180
TaiI ACGT 1 cut(s) 435
TaqI TCGA 1 cut(s) 1126
TaqII GACCGA 1 cut(s) 1199
TasI AATT 9 cut(s) 133, 193, 232, 359, 624, 900, 945, 959, 1349
TatI WGTACW 1 cut(s) 3
TfiI GAWTC 2 cut(s) 841, 994
Tru1I TTAA 6 cut(s) 219, 426, 441, 837, 1014, 1154
Tru9I TTAA 6 cut(s) 219, 426, 441, 837, 1014, 1154
TseFI GTSAC 1 cut(s) 1180
TseI GCWGC 5 cut(s) 25, 43, 168, 928, 1007
Tsp45I GTSAC 1 cut(s) 1180
TspDTI ATGAA 4 cut(s) 405, 516, 692, 1305
TspGWI ACGGA 2 cut(s) 609, 1179
Van91I CCANNNNNTGG 2 cut(s) 476, 540
Vha464I CTTAAG 1 cut(s) 836
VspI ATTAAT 1 cut(s) 1154
XagI CCTNNNNNAGG 1 cut(s) 1250
XapI RAATTY 3 cut(s) 232, 959, 1349
XceI RCATGY 1 cut(s) 10
XmnI GAANNNNTTC 1 cut(s) 699
XspI CTAG 4 cut(s) 717, 858, 881, 932
Zsp2I ATGCAT 1 cut(s) 1261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.