MD09G1252900.v1.1

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012735
Physical Location & Seq
Forward (+)
32405681 .. 32410472
4792 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1252900.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGCGCCCAAGAAGCGGCAGCTGCCAGGCGTCGACGAGAAACTGCAGAAGCTTCTGGATGCAAACATGGATGAAGCTCCCGCCAGACGGCGCGCTCGTGAGGCCTTCAAGGAAATTCAGCTTGGGATTGACCACATTCTGTTCAAGACACCGACTGATGGATTGAAAATGGATGAGTCATATGAGGTGAACTCCAGGGGACTGGAGATCTTCTGCAAGAGTTGGCTTCCAGCGACATCTCGTCCCAAAGCAATAGTGTGTTACTGTCATGGCTACGGAGATACTTGCACTTTTTTCTTTGAAGGCATGAAGCTGGCATCAAATGGGTACGGAGTTTTTGCAATGGATTACCCTGGCTTTGGTCTCTCAGAAGGTCTTCATTGCCATATTCCCAACTTTGACAGACTTGTAGATGATGTCATTGAGCATTACTCCAAAGTCAAAGAGCAGCCTGAGTTCCGTACACTCCCGACCTTTCTCTTTGGACAGTCCTTGGGTGGAGCTGTAGCACTGAAGGCTCACCTAAAACAACCTAGTGCATGGAATGGCGCAATATTAGTTGCACCAATGTGCAAGATTGCAGATGACATGGTTCCACCATGGCTACTAACACAAATCCTGATTGGGGTGGCCAAGTTCCTTCCAAAGCAAAAATTGGTTCCACAAAAGAATCTGGCTGAGGCAGCATTTAGAGACTTGAAGAAAAGAGAAATGACAGCCTATAATGTTATTGCTTACAAAGATAAACCACGCCTGGAAACTGCTCTAGAAATGCTCCGAACTACTGAAGAGATAGAACACCAATTGGAAGAAGTCTCTCTTCCATTATTAATCCTGCATGGAGAGGCTGACATTGTAACTGATCCAGCTGTAAGTAAGGCCCTGCACGAAAAGGCAAGCAGTTCCGACAAGAAGCTCATCCTTTACATGGATGCATATCATTCCCTTTTGGAGGGTGAGCCAGATGAAATGATAATTCGAGTTCTTAGTGACATCATTTCATGGCTTAATGAACACAGCGAAAAACTAATGGGCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

347

Amino Acids

38.97

Weight (kDa)

5.92

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 81 - 321 4.3e-62 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 86 - 320 4.6e-13 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 89 - 324 1.1e-11 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 33
AccII CGCG 1 cut(s) 93
AciI CCGC 2 cut(s) 16, 81
AclWI GGATC 1 cut(s) 857
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 1 cut(s) 114
AcuI CTGAAG 2 cut(s) 533, 807
AcyI GRCGYC 2 cut(s) 5, 30
AfaI GTAC 2 cut(s) 329, 463
AfiI CCNNNNNNNGG 7 cut(s) 15, 87, 158, 359, 625, 928, 952
AgsI TTSAA 5 cut(s) 109, 145, 166, 302, 700
AhdI GACNNNNNGTC 1 cut(s) 240
AjnI CCWGG 4 cut(s) 25, 194, 352, 753
AjuI GAANNNNNNNTTGG 2 cut(s) 105, 137
AleI CACNNNNGTG 1 cut(s) 568
AluBI AGCT 9 cut(s) 22, 52, 77, 121, 313, 503, 869, 916, 1038
AluI AGCT 9 cut(s) 22, 52, 77, 121, 313, 503, 869, 916, 1038
Alw26I GTCTC 3 cut(s) 368, 687, 820
AlwI GGATC 1 cut(s) 857
AoxI GGCC 3 cut(s) 102, 630, 879
ApeKI GCWGC 5 cut(s) 19, 22, 448, 683, 1035
ApoI RAATTY 1 cut(s) 114
AseI ATTAAT 1 cut(s) 830
Asp700I GAANNNNTTC 1 cut(s) 375
AspLEI GCGC 4 cut(s) 7, 93, 95, 551
AspS9I GGNCC 1 cut(s) 880
AsuHPI GGTGA 3 cut(s) 199, 512, 968
BalI TGGCCA 1 cut(s) 632
BanI GGYRCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 96
BbsI GAAGAC 1 cut(s) 368
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 4 cut(s) 9, 31, 460, 695
BccI CCATC 1 cut(s) 152
BceAI ACGGC 1 cut(s) 104
BciT130I CCWGG 4 cut(s) 27, 196, 354, 755
BcoDI GTCTC 3 cut(s) 368, 687, 820
BfaI CTAG 2 cut(s) 534, 767
BfmI CTRYAG 2 cut(s) 44, 504
BfoI RGCGCY 1 cut(s) 8
BglII AGATCT 1 cut(s) 207
BisI GCNGC 6 cut(s) 17, 20, 23, 449, 684, 1036
BlsI GCNGC 6 cut(s) 18, 21, 24, 450, 685, 1037
Bme1390I CCNGG 4 cut(s) 27, 196, 354, 755
BmeRI GACNNNNNGTC 1 cut(s) 240
BmgT120I GGNCC 1 cut(s) 880
BmiI GGNNCC 3 cut(s) 6, 594, 660
BmrFI CCNGG 4 cut(s) 27, 196, 354, 755
BmsI GCATC 3 cut(s) 49, 326, 922
BpiI GAAGAC 1 cut(s) 368
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 416, 448
BpmI CTGGAG 2 cut(s) 178, 224
Bpu10I CCTNAGC 1 cut(s) 678
BsaBI GATNNNNATC 1 cut(s) 936
BsaHI GRCGYC 2 cut(s) 5, 30
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 4 cut(s) 195, 352, 492, 599
Bsc4I CCNNNNNNNGG 7 cut(s) 15, 87, 158, 359, 625, 928, 952
Bse1I ACTGG 1 cut(s) 207
Bse3DI GCAATG 2 cut(s) 348, 379
Bse8I GATNNNNATC 1 cut(s) 936
BseBI CCWGG 4 cut(s) 27, 196, 354, 755
BseDI CCNNGG 4 cut(s) 195, 352, 492, 599
BseGI GGATG 5 cut(s) 64, 76, 178, 918, 937
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 7 cut(s) 15, 87, 158, 359, 625, 928, 952
BseMI GCAATG 2 cut(s) 348, 379
BseMII CTCAG 3 cut(s) 381, 444, 669
BseNI ACTGG 1 cut(s) 207
BsePI GCGCGC 1 cut(s) 91
BseXI GCAGC 4 cut(s) 9, 31, 460, 695
BsgI GTGCAG 1 cut(s) 869
Bsh1236I CGCG 1 cut(s) 93
BshFI GGCC 3 cut(s) 104, 632, 881
BshNI GGYRCC 1 cut(s) 4
BslFI GGGAC 2 cut(s) 213, 228
BslI CCNNNNNNNGG 7 cut(s) 15, 87, 158, 359, 625, 928, 952
BsmAI GTCTC 3 cut(s) 368, 687, 820
BsmFI GGGAC 2 cut(s) 213, 228
BsnI GGCC 3 cut(s) 104, 632, 881
Bso31I GGTCTC 1 cut(s) 368
Bsp143I GATC 2 cut(s) 207, 862
Bsp19I CCATGG 1 cut(s) 599
BspACI CCGC 2 cut(s) 16, 81
BspANI GGCC 3 cut(s) 104, 632, 881
BspCNI CTCAG 3 cut(s) 380, 445, 670
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 3 cut(s) 6, 594, 660
BspMAI CTGCAG 1 cut(s) 48
BspPI GGATC 1 cut(s) 857
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 368
BsrDI GCAATG 2 cut(s) 348, 379
BsrI ACTGG 1 cut(s) 207
BssECI CCNNGG 4 cut(s) 195, 352, 492, 599
BssHII GCGCGC 1 cut(s) 91
BssMI GATC 2 cut(s) 207, 862
BssNI GRCGYC 2 cut(s) 5, 30
BssSI CACGAG 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 492, 599
Bst2BI CACGAG 1 cut(s) 96
Bst2UI CCWGG 4 cut(s) 27, 196, 354, 755
Bst4CI ACNGT 2 cut(s) 266, 489
Bst6I CTCTTC 2 cut(s) 783, 825
BstACI GRCGYC 2 cut(s) 5, 30
BstC8I GCNNGC 3 cut(s) 93, 315, 898
BstDEI CTNAG 4 cut(s) 367, 453, 678, 986
BstDSI CCRYGG 1 cut(s) 599
BstENI CCTNNNNNAGG 1 cut(s) 950
BstF5I GGATG 5 cut(s) 64, 76, 178, 918, 937
BstFNI CGCG 1 cut(s) 93
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 4 cut(s) 7, 93, 95, 551
BstKTI GATC 2 cut(s) 210, 865
BstMAI GTCTC 3 cut(s) 368, 687, 820
BstMBI GATC 2 cut(s) 207, 862
BstMWI GCNNNNNNNGC 5 cut(s) 13, 22, 101, 515, 683
BstNI CCWGG 4 cut(s) 27, 196, 354, 755
BstSCI CCNGG 4 cut(s) 25, 194, 352, 753
BstSFI CTRYAG 2 cut(s) 44, 504
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 4 cut(s) 9, 31, 460, 695
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 1 cut(s) 207
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 1 cut(s) 207
BsuRI GGCC 3 cut(s) 104, 632, 881
BtgI CCRYGG 1 cut(s) 599
BtsCI GGATG 5 cut(s) 64, 76, 178, 918, 937
BtsIMutI CAGTG 1 cut(s) 509
Cac8I GCNNGC 3 cut(s) 93, 315, 898
CfoI GCGC 4 cut(s) 7, 93, 95, 551
Cfr13I GGNCC 1 cut(s) 880
CseI GACGC 1 cut(s) 19
Csp6I GTAC 2 cut(s) 328, 462
CviAII CATG 9 cut(s) 67, 269, 307, 540, 589, 600, 839, 928, 1002
CviQI GTAC 2 cut(s) 328, 462
DdeI CTNAG 4 cut(s) 367, 453, 678, 986
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 209, 864
DpnII GATC 2 cut(s) 207, 862
DriI GACNNNNNGTC 1 cut(s) 240
EaeI YGGCCR 1 cut(s) 630
Eam1104I CTCTTC 2 cut(s) 783, 825
Eam1105I GACNNNNNGTC 1 cut(s) 240
EarI CTCTTC 2 cut(s) 783, 825
Eco130I CCWWGG 2 cut(s) 492, 599
Eco147I AGGCCT 1 cut(s) 104
Eco31I GGTCTC 1 cut(s) 368
Eco57I CTGAAG 2 cut(s) 533, 807
EcoNI CCTNNNNNAGG 1 cut(s) 950
EcoO109I RGGNCCY 1 cut(s) 880
EcoRII CCWGG 4 cut(s) 25, 194, 352, 753
EcoT14I CCWWGG 2 cut(s) 492, 599
EcoT22I ATGCAT 1 cut(s) 937
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 492, 599
FaeI CATG 9 cut(s) 70, 272, 310, 543, 592, 603, 842, 931, 1005
FalI AAGNNNNNCTT 2 cut(s) 804, 836
FaqI GGGAC 2 cut(s) 213, 228
FatI CATG 9 cut(s) 66, 268, 306, 539, 588, 599, 838, 927, 1001
FauI CCCGC 1 cut(s) 88
FauNDI CATATG 1 cut(s) 181
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 6 cut(s) 17, 20, 23, 449, 684, 1036
FokI GGATG 5 cut(s) 71, 83, 185, 905, 944
Fsp4HI GCNGC 6 cut(s) 17, 20, 23, 449, 684, 1036
FspBI CTAG 2 cut(s) 534, 767
GlaI GCGC 4 cut(s) 6, 92, 94, 550
GluI GCNGC 6 cut(s) 17, 20, 23, 449, 684, 1036
GsuI CTGGAG 2 cut(s) 178, 224
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 104, 632, 881
HgaI GACGC 1 cut(s) 19
HhaI GCGC 4 cut(s) 7, 93, 95, 551
Hin1I GRCGYC 2 cut(s) 5, 30
Hin1II CATG 9 cut(s) 70, 272, 310, 543, 592, 603, 842, 931, 1005
Hin6I GCGC 4 cut(s) 5, 91, 93, 549
HinP1I GCGC 4 cut(s) 5, 91, 93, 549
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 2 cut(s) 176, 670
HphI GGTGA 3 cut(s) 199, 512, 968
Hpy166II GTNNAC 3 cut(s) 34, 190, 464
Hpy188I TCNGA 3 cut(s) 370, 779, 907
Hpy188III TCNNGA 6 cut(s) 56, 98, 145, 469, 619, 767
Hpy8I GTNNAC 3 cut(s) 34, 190, 464
Hpy99I CGWCG 2 cut(s) 35, 38
HpyAV CCTTC 5 cut(s) 115, 296, 365, 508, 650
HpyCH4III ACNGT 2 cut(s) 266, 489
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 22, 101, 515, 683
HpyF3I CTNAG 4 cut(s) 367, 453, 678, 986
Hsp92I GRCGYC 2 cut(s) 5, 30
Hsp92II CATG 9 cut(s) 70, 272, 310, 543, 592, 603, 842, 931, 1005
HspAI GCGC 4 cut(s) 5, 91, 93, 549
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 2 cut(s) 207, 862
LmnI GCTCC 3 cut(s) 82, 500, 780
Lsp1109I GCAGC 4 cut(s) 9, 31, 460, 695
LweI GCATC 3 cut(s) 49, 326, 922
MaeI CTAG 2 cut(s) 534, 767
MaeIII GTNAC 3 cut(s) 260, 856, 989
MalI GATC 2 cut(s) 209, 864
MboI GATC 2 cut(s) 207, 862
MboII GAAGA 6 cut(s) 202, 368, 712, 800, 812, 821
MfeI CAATTG 1 cut(s) 803
MflI RGATCY 1 cut(s) 207
MlsI TGGCCA 1 cut(s) 632
MluCI AATT 4 cut(s) 114, 653, 803, 975
MluNI TGGCCA 1 cut(s) 632
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 930
MnlI CCTC 5 cut(s) 94, 178, 673, 838, 946
Mox20I TGGCCA 1 cut(s) 632
Mph1103I ATGCAT 1 cut(s) 937
MroXI GAANNNNTTC 1 cut(s) 375
MscI TGGCCA 1 cut(s) 632
MseI TTAA 2 cut(s) 830, 1008
MslI CAYNNNNRTG 1 cut(s) 568
Msp20I TGGCCA 1 cut(s) 632
MspA1I CMGCKG 2 cut(s) 22, 869
MspR9I CCNGG 4 cut(s) 27, 196, 354, 755
MunI CAATTG 1 cut(s) 803
MvaI CCWGG 4 cut(s) 27, 196, 354, 755
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 5 cut(s) 13, 22, 101, 515, 683
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 599
NdeI CATATG 1 cut(s) 181
NdeII GATC 2 cut(s) 207, 862
NlaIII CATG 9 cut(s) 70, 272, 310, 543, 592, 603, 842, 931, 1005
NlaIV GGNNCC 3 cut(s) 6, 594, 660
NmuCI GTSAC 1 cut(s) 989
NsiI ATGCAT 1 cut(s) 937
OliI CACNNNNGTG 1 cut(s) 568
PauI GCGCGC 1 cut(s) 91
PceI AGGCCT 1 cut(s) 104
PcsI WCGNNNNNNNCGW 1 cut(s) 94
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 1 cut(s) 670
PkrI GCNGC 6 cut(s) 18, 21, 24, 450, 685, 1037
PleI GAGTC 1 cut(s) 184
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 184
PshBI ATTAAT 1 cut(s) 830
Psp6I CCWGG 4 cut(s) 25, 194, 352, 753
PspGI CCWGG 4 cut(s) 25, 194, 352, 753
PspN4I GGNNCC 3 cut(s) 6, 594, 660
PspPI GGNCC 1 cut(s) 880
PstI CTGCAG 1 cut(s) 48
PsuI RGATCY 1 cut(s) 207
PteI GCGCGC 1 cut(s) 91
PvuII CAGCTG 2 cut(s) 22, 869
RsaI GTAC 2 cut(s) 329, 463
RsaNI GTAC 2 cut(s) 328, 462
RseI CAYNNNNRTG 1 cut(s) 568
SalI GTCGAC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 830, 1008
SatI GCNGC 6 cut(s) 17, 20, 23, 449, 684, 1036
Sau3AI GATC 2 cut(s) 207, 862
Sau96I GGNCC 1 cut(s) 880
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 4 cut(s) 27, 196, 354, 755
SfaNI GCATC 3 cut(s) 49, 326, 922
SfcI CTRYAG 2 cut(s) 44, 504
SfoI GGCGCC 1 cut(s) 6
SgrDI CGTCGACG 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 568
Sse9I AATT 4 cut(s) 114, 653, 803, 975
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 2 cut(s) 16, 81
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 555
SspMI CTAG 2 cut(s) 534, 767
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 4 cut(s) 25, 194, 352, 753
StyI CCWWGG 2 cut(s) 492, 599
TaaI ACNGT 2 cut(s) 266, 489
TaqI TCGA 2 cut(s) 33, 979
TasI AATT 4 cut(s) 114, 653, 803, 975
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 1 cut(s) 670
Tru1I TTAA 2 cut(s) 830, 1008
Tru9I TTAA 2 cut(s) 830, 1008
TscAI CASTG 1 cut(s) 516
TseFI GTSAC 1 cut(s) 989
TseI GCWGC 5 cut(s) 19, 22, 448, 683, 1035
Tsp45I GTSAC 1 cut(s) 989
TspDTI ATGAA 6 cut(s) 87, 323, 368, 981, 990, 1026
TspGWI ACGGA 3 cut(s) 291, 345, 449
TspRI CASTG 1 cut(s) 516
VspI ATTAAT 1 cut(s) 830
XagI CCTNNNNNAGG 1 cut(s) 950
XapI RAATTY 1 cut(s) 114
XbaI TCTAGA 1 cut(s) 766
XmiI GTMKAC 1 cut(s) 33
XmnI GAANNNNTTC 1 cut(s) 375
XspI CTAG 2 cut(s) 534, 767
Zsp2I ATGCAT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.