MD17G1244600.v1.1

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012735
Physical Location & Seq
Forward (+)
29299235 .. 29304001
4767 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1244600.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGCGCCCAAGAAGCGGCAGCTGCCAGGCGTCGACGAGAAACTGCAGAAGCTTCTGGATGCAAACATGGATGAAGCTCCCGCCAGAAGGCGGGCTCGTGAGGCCTTCAAGGAAATTCAGCTTGGGATTGACCATATTCTATTCAAGACACCGACCGATGGATTGAAAATGGAGGAGTCATATGAGGTGAACTCCAGGGGATTGAAAATATTCTGCAAGAGTTGGCTTCCAGCGACATCTCGTCCCAAAGCAATAGTGTGTTACTGTCATGGCTACGGGGATACTTGCACTTTTTTCTTTGAAGGCATGAAGTTGGCATCAAATGGTTACGGAGTTTTTGCAATGGATTACCCTGGCTTTGGTCTCTCAGAAGGTCTACATGGCCATATTCCCAGCTTTGACAGACTTGTAGATGATGTCATTGAGCATTACTCAAAAGTCAAAGAGCAGCCTGAGTTACGTGGGCTCCCGACCTTTCTCTTTGGACAGTCCTTGGGTGGAGCTGTAGCACTGAAGGCTCACCTAAAACAACCTAGTGCATGGAATGGCGCAATACTAGTTGCACCGATGTGCAAGATTTCGGAGGACATGGTTCCACCATGGATACTAACACAAATCCTGATTGGTGTGGCTAAGTTCCTTCCAAAGAAAAAATTGGTTCCGCAAAAGAATCTGGCTGAGGCAGCATTTAAAGACTTGAAGAAAAGAGAAATGACAGCCTACAATGTTATTTCCTACAAGGATAAACCACGCCTGCAGACTGCTTTAGAAATGCTCCGAACTACTGAAGAGATAGAACGCCGATTGGAAGAAGTCTCTCTTCCATTATTAATCCTGCATGGAGAGGCTGATATTGTAACTGATCCATCTGTAAGTAAGGCCCTGTATGAGAAAGCAAGCAGTTCCGACAAGAAGCTCATCCTTTACATGGATGCATGGCATTCCCTTCTGGAGGGTGAGTCAGATGCAATGATAAATCGAGTTTTTAGCGACATCCTTTCATGGCTTAGTGAGCACAGTGAAAAATTAACAGGCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

347

Amino Acids

38.87

Weight (kDa)

7.12

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 81 - 320 1.6e-63 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 86 - 317 3.8e-11 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 89 - 320 1.4e-11 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 2 cut(s) 33, 376
AciI CCGC 4 cut(s) 16, 81, 91, 662
AclWI GGATC 1 cut(s) 857
AcoI YGGCCR 1 cut(s) 382
AcsI RAATTY 1 cut(s) 114
AcuI CTGAAG 2 cut(s) 533, 807
AcyI GRCGYC 2 cut(s) 5, 30
AfiI CCNNNNNNNGG 7 cut(s) 15, 87, 90, 158, 359, 928, 952
AgsI TTSAA 6 cut(s) 109, 145, 166, 205, 302, 700
AhdI GACNNNNNGTC 1 cut(s) 240
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 3 cut(s) 25, 194, 352
AjuI GAANNNNNNNTTGG 2 cut(s) 105, 137
AleI CACNNNNGTG 1 cut(s) 568
AluBI AGCT 8 cut(s) 22, 52, 77, 121, 396, 503, 916, 1038
AluI AGCT 8 cut(s) 22, 52, 77, 121, 396, 503, 916, 1038
Alw21I GWGCWC 1 cut(s) 1017
Alw26I GTCTC 2 cut(s) 368, 820
AlwI GGATC 1 cut(s) 857
AoxI GGCC 3 cut(s) 102, 382, 879
ApeKI GCWGC 5 cut(s) 19, 22, 448, 683, 1035
ApoI RAATTY 1 cut(s) 114
AseI ATTAAT 1 cut(s) 830
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 2 cut(s) 7, 551
AspS9I GGNCC 1 cut(s) 880
AsuHPI GGTGA 3 cut(s) 199, 512, 968
BalI TGGCCA 1 cut(s) 384
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 97, 468
BauI CACGAG 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 1017
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 4 cut(s) 9, 31, 460, 695
BccI CCATC 2 cut(s) 152, 874
BciT130I CCWGG 3 cut(s) 27, 196, 354
BciVI GTATCC 2 cut(s) 274, 597
BcoDI GTCTC 2 cut(s) 368, 820
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 2 cut(s) 534, 557
BfmI CTRYAG 3 cut(s) 44, 504, 755
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 2 cut(s) 274, 597
BisI GCNGC 6 cut(s) 17, 20, 23, 449, 684, 1036
BlsI GCNGC 6 cut(s) 18, 21, 24, 450, 685, 1037
Bme1390I CCNGG 3 cut(s) 27, 196, 354
BmeRI GACNNNNNGTC 1 cut(s) 240
BmgT120I GGNCC 1 cut(s) 880
BmiI GGNNCC 4 cut(s) 6, 467, 594, 660
BmrFI CCNGG 3 cut(s) 27, 196, 354
BmsI GCATC 4 cut(s) 49, 326, 922, 955
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 416, 448
BpmI CTGGAG 2 cut(s) 178, 971
Bpu10I CCTNAGC 1 cut(s) 678
BsaAI YACGTR 1 cut(s) 461
BsaHI GRCGYC 2 cut(s) 5, 30
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 4 cut(s) 195, 352, 492, 599
BsaXI ACNNNNNCTCC 4 cut(s) 450, 480, 944, 974
Bsc4I CCNNNNNNNGG 7 cut(s) 15, 87, 90, 158, 359, 928, 952
Bse3DI GCAATG 2 cut(s) 348, 975
BseBI CCWGG 3 cut(s) 27, 196, 354
BseDI CCNNGG 4 cut(s) 195, 352, 492, 599
BseGI GGATG 5 cut(s) 64, 76, 918, 937, 993
BseLI CCNNNNNNNGG 7 cut(s) 15, 87, 90, 158, 359, 928, 952
BseMI GCAATG 2 cut(s) 348, 975
BseMII CTCAG 3 cut(s) 381, 444, 669
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 4 cut(s) 9, 31, 460, 695
BseYI CCCAGC 1 cut(s) 392
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 3 cut(s) 104, 384, 881
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 156
BsiHKAI GWGCWC 1 cut(s) 1017
BslFI GGGAC 1 cut(s) 228
BslI CCNNNNNNNGG 7 cut(s) 15, 87, 90, 158, 359, 928, 952
BsmAI GTCTC 2 cut(s) 368, 820
BsmFI GGGAC 1 cut(s) 228
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 3 cut(s) 104, 384, 881
Bso31I GGTCTC 1 cut(s) 368
Bsp1286I GDGCHC 3 cut(s) 97, 468, 1017
Bsp143I GATC 1 cut(s) 862
Bsp19I CCATGG 1 cut(s) 599
BspACI CCGC 4 cut(s) 16, 81, 91, 662
BspANI GGCC 3 cut(s) 104, 384, 881
BspCNI CTCAG 3 cut(s) 380, 445, 670
BspLI GGNNCC 4 cut(s) 6, 467, 594, 660
BspMAI CTGCAG 2 cut(s) 48, 759
BspPI GGATC 1 cut(s) 857
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 368
BsrDI GCAATG 2 cut(s) 348, 975
BssECI CCNNGG 4 cut(s) 195, 352, 492, 599
BssMI GATC 1 cut(s) 862
BssNI GRCGYC 2 cut(s) 5, 30
BssSI CACGAG 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 492, 599
Bst2BI CACGAG 1 cut(s) 96
Bst2UI CCWGG 3 cut(s) 27, 196, 354
Bst4CI ACNGT 3 cut(s) 266, 489, 1019
Bst6I CTCTTC 2 cut(s) 783, 825
BstACI GRCGYC 2 cut(s) 5, 30
BstBAI YACGTR 1 cut(s) 461
BstC8I GCNNGC 3 cut(s) 93, 755, 898
BstDEI CTNAG 5 cut(s) 367, 453, 633, 678, 1007
BstDSI CCRYGG 1 cut(s) 599
BstENI CCTNNNNNAGG 1 cut(s) 950
BstF5I GGATG 5 cut(s) 64, 76, 918, 937, 993
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 551
BstKTI GATC 1 cut(s) 865
BstMAI GTCTC 2 cut(s) 368, 820
BstMBI GATC 1 cut(s) 862
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 6 cut(s) 13, 22, 101, 515, 683, 1012
BstNI CCWGG 3 cut(s) 27, 196, 354
BstSCI CCNGG 3 cut(s) 25, 194, 352
BstSFI CTRYAG 3 cut(s) 44, 504, 755
BstV1I GCAGC 4 cut(s) 9, 31, 460, 695
BsuI GTATCC 2 cut(s) 274, 597
BsuRI GGCC 3 cut(s) 104, 384, 881
BtgI CCRYGG 1 cut(s) 599
BtsCI GGATG 5 cut(s) 64, 76, 918, 937, 993
BtsIMutI CAGTG 2 cut(s) 509, 1024
Cac8I GCNNGC 3 cut(s) 93, 755, 898
CfoI GCGC 2 cut(s) 7, 551
Cfr13I GGNCC 1 cut(s) 880
CseI GACGC 1 cut(s) 19
DdeI CTNAG 5 cut(s) 367, 453, 633, 678, 1007
DinI GGCGCC 1 cut(s) 6
DpnI GATC 1 cut(s) 864
DpnII GATC 1 cut(s) 862
DraI TTTAAA 1 cut(s) 691
DriI GACNNNNNGTC 1 cut(s) 240
EaeI YGGCCR 1 cut(s) 382
Eam1104I CTCTTC 2 cut(s) 783, 825
Eam1105I GACNNNNNGTC 1 cut(s) 240
EarI CTCTTC 2 cut(s) 783, 825
Eco130I CCWWGG 2 cut(s) 492, 599
Eco147I AGGCCT 1 cut(s) 104
Eco24I GRGCYC 2 cut(s) 97, 468
Eco31I GGTCTC 1 cut(s) 368
Eco57I CTGAAG 2 cut(s) 533, 807
EcoNI CCTNNNNNAGG 1 cut(s) 950
EcoO109I RGGNCCY 1 cut(s) 880
EcoRII CCWGG 3 cut(s) 25, 194, 352
EcoT14I CCWWGG 2 cut(s) 492, 599
EcoT22I ATGCAT 1 cut(s) 937
EcoT38I GRGCYC 2 cut(s) 97, 468
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 492, 599
FalI AAGNNNNNCTT 2 cut(s) 804, 836
FaqI GGGAC 1 cut(s) 228
FauI CCCGC 2 cut(s) 84, 88
FauNDI CATATG 1 cut(s) 181
FblI GTMKAC 2 cut(s) 33, 376
Fnu4HI GCNGC 6 cut(s) 17, 20, 23, 449, 684, 1036
FokI GGATG 5 cut(s) 71, 83, 905, 944, 980
FriOI GRGCYC 2 cut(s) 97, 468
Fsp4HI GCNGC 6 cut(s) 17, 20, 23, 449, 684, 1036
FspBI CTAG 2 cut(s) 534, 557
GlaI GCGC 2 cut(s) 6, 550
GluI GCNGC 6 cut(s) 17, 20, 23, 449, 684, 1036
GsaI CCCAGC 1 cut(s) 396
GsuI CTGGAG 2 cut(s) 178, 971
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 104, 384, 881
HgaI GACGC 1 cut(s) 19
HhaI GCGC 2 cut(s) 7, 551
Hin1I GRCGYC 2 cut(s) 5, 30
Hin6I GCGC 2 cut(s) 5, 549
HinP1I GCGC 2 cut(s) 5, 549
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 3 cut(s) 176, 670, 959
HphI GGTGA 3 cut(s) 199, 512, 968
Hpy166II GTNNAC 3 cut(s) 34, 190, 377
Hpy188I TCNGA 5 cut(s) 370, 583, 779, 907, 964
Hpy188III TCNNGA 6 cut(s) 56, 98, 145, 469, 619, 950
Hpy8I GTNNAC 3 cut(s) 34, 190, 377
Hpy99I CGWCG 2 cut(s) 35, 38
HpyAV CCTTC 7 cut(s) 81, 115, 296, 365, 508, 650, 956
HpyCH4III ACNGT 3 cut(s) 266, 489, 1019
HpyCH4IV ACGT 1 cut(s) 460
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 22, 101, 515, 683, 1012
HpyF3I CTNAG 5 cut(s) 367, 453, 633, 678, 1007
HpySE526I ACGT 1 cut(s) 460
Hsp92I GRCGYC 2 cut(s) 5, 30
HspAI GCGC 2 cut(s) 5, 549
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 1 cut(s) 862
LmnI GCTCC 4 cut(s) 82, 471, 500, 780
Lsp1109I GCAGC 4 cut(s) 9, 31, 460, 695
LweI GCATC 4 cut(s) 49, 326, 922, 955
MaeI CTAG 2 cut(s) 534, 557
MaeII ACGT 1 cut(s) 460
MaeIII GTNAC 4 cut(s) 260, 326, 456, 856
MalI GATC 1 cut(s) 864
MboI GATC 1 cut(s) 862
MboII GAAGA 4 cut(s) 712, 800, 812, 821
MhlI GDGCHC 3 cut(s) 97, 468, 1017
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 3 cut(s) 114, 653, 1025
MluNI TGGCCA 1 cut(s) 384
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 2 cut(s) 185, 968
MmeI TCCRAC 1 cut(s) 930
MnlI CCTC 7 cut(s) 94, 166, 178, 577, 673, 838, 946
Mox20I TGGCCA 1 cut(s) 384
Mph1103I ATGCAT 1 cut(s) 937
MroXI GAANNNNTTC 1 cut(s) 209
MscI TGGCCA 1 cut(s) 384
MseI TTAA 3 cut(s) 690, 830, 1028
MslI CAYNNNNRTG 1 cut(s) 568
Msp20I TGGCCA 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 22
MspR9I CCNGG 3 cut(s) 27, 196, 354
Mva1269I GAATGC 1 cut(s) 940
MvaI CCWGG 3 cut(s) 27, 196, 354
MwoI GCNNNNNNNGC 6 cut(s) 13, 22, 101, 515, 683, 1012
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 599
NdeI CATATG 1 cut(s) 181
NdeII GATC 1 cut(s) 862
NlaIV GGNNCC 4 cut(s) 6, 467, 594, 660
NsiI ATGCAT 1 cut(s) 937
OliI CACNNNNGTG 1 cut(s) 568
PceI AGGCCT 1 cut(s) 104
PctI GAATGC 1 cut(s) 940
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 1 cut(s) 670
PkrI GCNGC 6 cut(s) 18, 21, 24, 450, 685, 1037
PleI GAGTC 2 cut(s) 184, 967
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 2 cut(s) 184, 967
Ppu21I YACGTR 1 cut(s) 461
PshBI ATTAAT 1 cut(s) 830
Psp6I CCWGG 3 cut(s) 25, 194, 352
PspFI CCCAGC 1 cut(s) 392
PspGI CCWGG 3 cut(s) 25, 194, 352
PspN4I GGNNCC 4 cut(s) 6, 467, 594, 660
PspPI GGNCC 1 cut(s) 880
PstI CTGCAG 2 cut(s) 48, 759
PvuII CAGCTG 1 cut(s) 22
RseI CAYNNNNRTG 1 cut(s) 568
SalI GTCGAC 1 cut(s) 32
SaqAI TTAA 3 cut(s) 690, 830, 1028
SatI GCNGC 6 cut(s) 17, 20, 23, 449, 684, 1036
Sau3AI GATC 1 cut(s) 862
Sau96I GGNCC 1 cut(s) 880
SchI GAGTC 2 cut(s) 185, 968
ScrFI CCNGG 3 cut(s) 27, 196, 354
SduI GDGCHC 3 cut(s) 97, 468, 1017
SfaNI GCATC 4 cut(s) 49, 326, 922, 955
SfcI CTRYAG 3 cut(s) 44, 504, 755
SfoI GGCGCC 1 cut(s) 6
SgrDI CGTCGACG 1 cut(s) 32
SmiMI CAYNNNNRTG 1 cut(s) 568
SpeI ACTAGT 1 cut(s) 556
Sse9I AATT 3 cut(s) 114, 653, 1025
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 4 cut(s) 16, 81, 91, 662
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 210
SspMI CTAG 2 cut(s) 534, 557
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 3 cut(s) 25, 194, 352
StyI CCWWGG 2 cut(s) 492, 599
TaaI ACNGT 3 cut(s) 266, 489, 1019
TaiI ACGT 1 cut(s) 463
TaqI TCGA 2 cut(s) 33, 979
TaqII GACCGA 1 cut(s) 170
TasI AATT 3 cut(s) 114, 653, 1025
TauI GCSGC 1 cut(s) 19
TfiI GAWTC 1 cut(s) 670
Tru1I TTAA 3 cut(s) 690, 830, 1028
Tru9I TTAA 3 cut(s) 690, 830, 1028
TscAI CASTG 2 cut(s) 516, 1024
TseI GCWGC 5 cut(s) 19, 22, 448, 683, 1035
TspDTI ATGAA 3 cut(s) 87, 323, 990
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 2 cut(s) 516, 1024
VspI ATTAAT 1 cut(s) 830
XagI CCTNNNNNAGG 1 cut(s) 950
XapI RAATTY 1 cut(s) 114
XmiI GTMKAC 2 cut(s) 33, 376
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 2 cut(s) 534, 557
Zsp2I ATGCAT 1 cut(s) 937
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.