Rh5DG333000

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012735
Physical Location & Seq
Forward (+)
43990222 .. 43994808
4587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG333000.1

Sequence Viewer

Length: 1152 bp
ATGATTAAACACCAACTGAATTTCAAAATCCTCCAAAGAATAAAGAGTTCTAGGAAGTTCAGGCCCGAGTGTTGTTCCCCAACAAAGAGAAAGAAGAGCATAGAAGTAATGGCACCCCAAGTGAGGTTGCAAGGTGTTGATGATAAATTGCAGAAGCTTCTGGATACTAACATGGATGAAGCTCCGGCCAGAAGGAGAGCTCGTGAAGCTTTTAAGGACATTCAGCTTAATATTGATCATATATTATTCAAGACACCAACTGATGGACTGAAAATAAAAGAGTCATATGAAGTGAACTCCAGGGGATTGGAGATCTTCTCCAAGAGTTGGCTTCCAGAGACATCTCAGCCCAAAGCAATACTGTGTTACTGTCATGGTTACGGAGATACTTGCACATTTTTCTTTGAAGGAATTGCAAGGAAGTTGGCATCATATGGATATGGAGTTTTTGCAATGGACTACCCTGGATTTGGTCTTTCGGAAGGTCTTCATTGCTATATTCCTAGCTTTGAAATGCTCGTGGATGATGTCATTGAGCATTTCTCTAAAGTCAAAGAGCAACCTGAGTTCAATGCTCTCCCAAGCTTTCTCTTTGGGCAGTCCATGGGCGGAGCTGTAGCTCTTAAGATTCACCTAAAACAACCTAGTGGATGGAATGGTGCTATCCTAGTTGCACCTATGTGCAGAATTGCAGATGACATTTCCCCGCCGTGGCTACTAGCACAAGTCCTAATTGGTGTTGCTAAATTCCTTCCAAAGCAGAAGTTGGTTCCACAAAAGAATCTAGCTGAGGCAGCCTTTAAAGACTTGAAGAAACGGGAAATGACGGCCTATAATGTTGTTGCTTACAAGGATAAGCCACGCCTGCAGACTGCTGTAGAAATGCTCCGGACAACTCAAGAGATAGAAAGTCGATTAGAAGAAGTCTCTCTTCCATTATTAATCCTTCATGGGGAAGCTGATATTGTGACCGATCCATCTGTGAGTAAGGCGCTTTACGAGAAAGCAAGCAGTTCCGACAAGAAGCTAATCCTTTATGAGGATGCATATCATTCCCTTCTGGAAGGTGAGCCGGATGAAATGATAGTTCAAGTTTTCAATGACATCATATCATGGCTCAACGAACACAGCTCAAAATTTACAGCTAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

383

Amino Acids

43.44

Weight (kDa)

8.03

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 116 - 358 3.8e-64 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 121 - 357 1.9e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 124 - 361 1.6e-12 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 112
AccB7I CCANNNNNTGG 2 cut(s) 263, 327
AccIII TCCGGA 1 cut(s) 888
AciI CCGC 2 cut(s) 609, 707
AclWI GGATC 1 cut(s) 968
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 3 cut(s) 19, 746, 1136
AfiI CCNNNNNNNGG 7 cut(s) 123, 263, 327, 470, 711, 952, 1039
AflII CTTAAG 1 cut(s) 623
AgsI TTSAA 8 cut(s) 25, 250, 407, 512, 571, 811, 1091, 1099
AjnI CCWGG 2 cut(s) 299, 463
AleI CACNNNNGTG 1 cut(s) 679
Alw21I GWGCWC 1 cut(s) 202
Alw26I GTCTC 2 cut(s) 332, 931
AlwI GGATC 1 cut(s) 968
Ama87I CYCGRG 1 cut(s) 65
Aor13HI TCCGGA 1 cut(s) 888
AoxI GGCC 3 cut(s) 62, 186, 828
ApeKI GCWGC 1 cut(s) 794
ApoI RAATTY 3 cut(s) 19, 746, 1136
AseI ATTAAT 1 cut(s) 941
Asp700I GAANNNNTTC 1 cut(s) 486
AspLEI GCGC 1 cut(s) 994
AspS9I GGNCC 1 cut(s) 63
AsuHPI GGTGA 2 cut(s) 623, 1079
AvaI CYCGRG 1 cut(s) 65
BanI GGYRCC 1 cut(s) 112
BanII GRGCYC 1 cut(s) 202
BauI CACGAG 2 cut(s) 201, 518
BbsI GAAGAC 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 202
BbvCI CCTCAGC 1 cut(s) 789
BbvI GCAGC 1 cut(s) 806
BccI CCATC 3 cut(s) 257, 645, 985
BceAI ACGGC 2 cut(s) 694, 843
BciT130I CCWGG 2 cut(s) 301, 465
BciVI GTATCC 1 cut(s) 157
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 2 cut(s) 332, 931
BfaI CTAG 7 cut(s) 51, 504, 645, 668, 719, 785, 1146
BfmI CTRYAG 3 cut(s) 615, 866, 876
BfoI RGCGCY 1 cut(s) 995
BfrI CTTAAG 1 cut(s) 623
BfuI GTATCC 1 cut(s) 157
BglII AGATCT 1 cut(s) 312
BisI GCNGC 1 cut(s) 795
BlsI GCNGC 1 cut(s) 796
Bme1390I CCNGG 2 cut(s) 301, 465
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 63
BmiI GGNNCC 2 cut(s) 114, 771
BmrFI CCNGG 2 cut(s) 301, 465
BmsI GCATC 2 cut(s) 437, 1033
BpiI GAAGAC 1 cut(s) 479
BplI GAGNNNNNCTC 4 cut(s) 302, 334, 527, 559
BpmI CTGGAG 1 cut(s) 283
Bpu10I CCTNAGC 1 cut(s) 789
BpuEI CTTGAG 1 cut(s) 882
BsaBI GATNNNNATC 1 cut(s) 1047
BsaJI CCNNGG 4 cut(s) 300, 463, 603, 710
BsaWI WCCGGW 1 cut(s) 888
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bsc4I CCNNNNNNNGG 7 cut(s) 123, 263, 327, 470, 711, 952, 1039
Bse3DI GCAATG 2 cut(s) 459, 490
Bse8I GATNNNNATC 1 cut(s) 1047
BseAI TCCGGA 1 cut(s) 888
BseBI CCWGG 2 cut(s) 301, 465
BseDI CCNNGG 4 cut(s) 300, 463, 603, 710
BseGI GGATG 5 cut(s) 181, 529, 656, 1048, 1081
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 7 cut(s) 123, 263, 327, 470, 711, 952, 1039
BseMI GCAATG 2 cut(s) 459, 490
BseMII CTCAG 3 cut(s) 359, 555, 780
BseXI GCAGC 1 cut(s) 806
BsgI GTGCAG 1 cut(s) 703
BshFI GGCC 3 cut(s) 64, 188, 830
BshNI GGYRCC 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 202
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 3 cut(s) 185, 889, 1073
BslI CCNNNNNNNGG 7 cut(s) 123, 263, 327, 470, 711, 952, 1039
BsmAI GTCTC 2 cut(s) 332, 931
BsnI GGCC 3 cut(s) 64, 188, 830
BsoBI CYCGRG 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 202
Bsp13I TCCGGA 1 cut(s) 888
Bsp143I GATC 3 cut(s) 235, 312, 973
Bsp19I CCATGG 1 cut(s) 603
BspACI CCGC 2 cut(s) 609, 707
BspANI GGCC 3 cut(s) 64, 188, 830
BspCNI CTCAG 3 cut(s) 358, 556, 781
BspEI TCCGGA 1 cut(s) 888
BspLI GGNNCC 2 cut(s) 114, 771
BspMAI CTGCAG 1 cut(s) 870
BspPI GGATC 1 cut(s) 968
BspQI GCTCTTC 1 cut(s) 89
BspT107I GGYRCC 1 cut(s) 112
BspTI CTTAAG 1 cut(s) 623
BsrDI GCAATG 2 cut(s) 459, 490
BssECI CCNNGG 4 cut(s) 300, 463, 603, 710
BssMI GATC 3 cut(s) 235, 312, 973
BssSI CACGAG 2 cut(s) 201, 518
BssT1I CCWWGG 1 cut(s) 603
Bst2BI CACGAG 2 cut(s) 201, 518
Bst2UI CCWGG 2 cut(s) 301, 465
Bst4CI ACNGT 2 cut(s) 363, 371
Bst6I CTCTTC 2 cut(s) 89, 936
BstAFI CTTAAG 1 cut(s) 623
BstC8I GCNNGC 2 cut(s) 866, 1009
BstDEI CTNAG 3 cut(s) 345, 564, 789
BstDSI CCRYGG 2 cut(s) 603, 710
BstENI CCTNNNNNAGG 1 cut(s) 1037
BstF5I GGATG 5 cut(s) 181, 529, 656, 1048, 1081
BstH2I RGCGCY 1 cut(s) 995
BstHHI GCGC 1 cut(s) 994
BstKTI GATC 3 cut(s) 238, 315, 976
BstMAI GTCTC 2 cut(s) 332, 931
BstMBI GATC 3 cut(s) 235, 312, 973
BstMWI GCNNNNNNNGC 3 cut(s) 206, 794, 865
BstNI CCWGG 2 cut(s) 301, 465
BstSCI CCNGG 2 cut(s) 299, 463
BstSFI CTRYAG 3 cut(s) 615, 866, 876
BstV1I GCAGC 1 cut(s) 806
BstV2I GAAGAC 1 cut(s) 479
BstX2I RGATCY 1 cut(s) 312
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 1 cut(s) 312
BsuI GTATCC 1 cut(s) 157
BsuRI GGCC 3 cut(s) 64, 188, 830
BtgI CCRYGG 2 cut(s) 603, 710
BtsCI GGATG 5 cut(s) 181, 529, 656, 1048, 1081
Cac8I GCNNGC 2 cut(s) 866, 1009
CfoI GCGC 1 cut(s) 994
Cfr13I GGNCC 1 cut(s) 63
CviAII CATG 5 cut(s) 172, 374, 604, 950, 1113
DdeI CTNAG 3 cut(s) 345, 564, 789
DpnI GATC 3 cut(s) 237, 314, 975
DpnII GATC 3 cut(s) 235, 312, 973
DraI TTTAAA 1 cut(s) 802
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 2 cut(s) 89, 936
EarI CTCTTC 2 cut(s) 89, 936
EciI GGCGGA 1 cut(s) 624
Ecl136II GAGCTC 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 603
Eco24I GRGCYC 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 200
Eco88I CYCGRG 1 cut(s) 65
EcoICRI GAGCTC 1 cut(s) 200
EcoNI CCTNNNNNAGG 1 cut(s) 1037
EcoRII CCWGG 2 cut(s) 299, 463
EcoT14I CCWWGG 1 cut(s) 603
EcoT22I ATGCAT 1 cut(s) 1048
EcoT38I GRGCYC 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 5 cut(s) 175, 377, 607, 953, 1116
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FatI CATG 5 cut(s) 171, 373, 603, 949, 1112
FauI CCCGC 1 cut(s) 714
FauNDI CATATG 2 cut(s) 286, 433
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 795
FokI GGATG 5 cut(s) 188, 536, 663, 1055, 1088
FriOI GRGCYC 1 cut(s) 202
Fsp4HI GCNGC 1 cut(s) 795
FspBI CTAG 7 cut(s) 51, 504, 645, 668, 719, 785, 1146
GlaI GCGC 1 cut(s) 993
GluI GCNGC 1 cut(s) 795
GsuI CTGGAG 1 cut(s) 283
HaeII RGCGCY 1 cut(s) 995
HaeIII GGCC 3 cut(s) 64, 188, 830
HapII CCGG 3 cut(s) 185, 889, 1073
HhaI GCGC 1 cut(s) 994
Hin1II CATG 5 cut(s) 175, 377, 607, 953, 1116
Hin6I GCGC 1 cut(s) 992
HinP1I GCGC 1 cut(s) 992
HindIII AAGCTT 3 cut(s) 155, 207, 583
HinfI GANTC 3 cut(s) 281, 628, 781
HpaII CCGG 3 cut(s) 185, 889, 1073
HphI GGTGA 2 cut(s) 623, 1079
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 2 cut(s) 481, 1018
Hpy188III TCNNGA 7 cut(s) 161, 203, 250, 335, 889, 899, 1061
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 7 cut(s) 186, 401, 476, 761, 956, 1058, 1067
HpyCH4III ACNGT 2 cut(s) 363, 371
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 794, 865
HpyF3I CTNAG 3 cut(s) 345, 564, 789
Hsp92II CATG 5 cut(s) 175, 377, 607, 953, 1116
HspAI GCGC 1 cut(s) 992
Kpn2I TCCGGA 1 cut(s) 888
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 3 cut(s) 235, 312, 973
LguI GCTCTTC 1 cut(s) 89
LmnI GCTCC 3 cut(s) 187, 611, 891
Lsp1109I GCAGC 1 cut(s) 806
LweI GCATC 2 cut(s) 437, 1033
MaeI CTAG 7 cut(s) 51, 504, 645, 668, 719, 785, 1146
MaeIII GTNAC 3 cut(s) 365, 377, 967
MalI GATC 3 cut(s) 237, 314, 975
MboI GATC 3 cut(s) 235, 312, 973
MboII GAAGA 6 cut(s) 106, 307, 479, 823, 923, 932
MflI RGATCY 1 cut(s) 312
MhlI GDGCHC 1 cut(s) 202
MluCI AATT 7 cut(s) 19, 146, 411, 687, 732, 746, 1136
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 1041
MnlI CCTC 4 cut(s) 41, 117, 784, 1033
Mph1103I ATGCAT 1 cut(s) 1048
MroI TCCGGA 1 cut(s) 888
MroXI GAANNNNTTC 1 cut(s) 486
MseI TTAA 6 cut(s) 6, 213, 228, 624, 801, 941
MslI CAYNNNNRTG 1 cut(s) 679
MspCI CTTAAG 1 cut(s) 623
MspI CCGG 3 cut(s) 185, 889, 1073
MspR9I CCNGG 2 cut(s) 301, 465
MvaI CCWGG 2 cut(s) 301, 465
MwoI GCNNNNNNNGC 3 cut(s) 206, 794, 865
NcoI CCATGG 1 cut(s) 603
NdeI CATATG 2 cut(s) 286, 433
NdeII GATC 3 cut(s) 235, 312, 973
NlaIII CATG 5 cut(s) 175, 377, 607, 953, 1116
NlaIV GGNNCC 2 cut(s) 114, 771
NmuCI GTSAC 1 cut(s) 967
NsiI ATGCAT 1 cut(s) 1048
OliI CACNNNNGTG 1 cut(s) 679
PciSI GCTCTTC 1 cut(s) 89
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 2 cut(s) 628, 781
PflMI CCANNNNNTGG 2 cut(s) 263, 327
PkrI GCNGC 1 cut(s) 796
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
PshBI ATTAAT 1 cut(s) 941
Psp124BI GAGCTC 1 cut(s) 202
Psp6I CCWGG 2 cut(s) 299, 463
PspGI CCWGG 2 cut(s) 299, 463
PspN4I GGNNCC 2 cut(s) 114, 771
PspPI GGNCC 1 cut(s) 63
PstI CTGCAG 1 cut(s) 870
PsuI RGATCY 1 cut(s) 312
RseI CAYNNNNRTG 1 cut(s) 679
SacI GAGCTC 1 cut(s) 202
SapI GCTCTTC 1 cut(s) 89
SaqAI TTAA 6 cut(s) 6, 213, 228, 624, 801, 941
SatI GCNGC 1 cut(s) 795
Sau3AI GATC 3 cut(s) 235, 312, 973
Sau96I GGNCC 1 cut(s) 63
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 301, 465
SduI GDGCHC 1 cut(s) 202
SfaNI GCATC 2 cut(s) 437, 1033
SfcI CTRYAG 3 cut(s) 615, 866, 876
SmiMI CAYNNNNRTG 1 cut(s) 679
SmlI CTYRAG 2 cut(s) 623, 897
SmoI CTYRAG 2 cut(s) 623, 897
Sse9I AATT 7 cut(s) 19, 146, 411, 687, 732, 746, 1136
SsiI CCGC 2 cut(s) 609, 707
SspI AATATT 1 cut(s) 232
SspMI CTAG 7 cut(s) 51, 504, 645, 668, 719, 785, 1146
SstI GAGCTC 1 cut(s) 202
StyD4I CCNGG 2 cut(s) 299, 463
StyI CCWWGG 1 cut(s) 603
TaaI ACNGT 2 cut(s) 363, 371
TaqI TCGA 1 cut(s) 913
TaqII GACCGA 1 cut(s) 986
TasI AATT 7 cut(s) 19, 146, 411, 687, 732, 746, 1136
TfiI GAWTC 2 cut(s) 628, 781
Tru1I TTAA 6 cut(s) 6, 213, 228, 624, 801, 941
Tru9I TTAA 6 cut(s) 6, 213, 228, 624, 801, 941
TseFI GTSAC 1 cut(s) 967
TseI GCWGC 1 cut(s) 794
Tsp45I GTSAC 1 cut(s) 967
TspDTI ATGAA 5 cut(s) 192, 303, 479, 938, 1092
TspGWI ACGGA 1 cut(s) 396
Van91I CCANNNNNTGG 2 cut(s) 263, 327
Vha464I CTTAAG 1 cut(s) 623
VspI ATTAAT 1 cut(s) 941
XagI CCTNNNNNAGG 1 cut(s) 1037
XapI RAATTY 3 cut(s) 19, 746, 1136
XmnI GAANNNNTTC 1 cut(s) 486
XspI CTAG 7 cut(s) 51, 504, 645, 668, 719, 785, 1146
Zsp2I ATGCAT 1 cut(s) 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.