FvH4_3g27052

Double-stranded RNA binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016795
Physical Location & Seq
Reverse (-)
20166766 .. 20168619
1854 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27052.t1

Sequence Viewer

Length: 1029 bp
ATGATGTACAAGAGTAATCTGCAGGAGCTGTGCCATGAAAAGAAGTGGGGGTTGCCGAGATACACCCCCATGAAAGATGGACCAGATCACAATCCTTGCTTCAGGGCTTGTGTCACCGTAAATGGCTTCTCATTTGACTCCCCAGTTGCCTGTAAATCTTCCAAACAAGCTCAAAACCAGGCCGCCATGGTCGCCTTTCTTCACTTCACCTCTCCACCTTCTTCTTCACCTGTGGATGAACATTGCACCTTGGAGAGCCCAGAGTTCATCAATAGTACTTTGACGGAAGCAGAGAATGCTGCTTCAGAGGCTGCTTCTATGTTTATTTCATTGTCAGAAGGGTTTCAGGATGACGAACCTGGTCGTCTTTACAAGAATCTCTTACAGGAGTTAGCTCAGAGAGAAGGTTTATCACTGCCAGCATATACAACTGTAAACTGGGGTGCATCTCACATGCCAACATTCTCTTCCACTGTGGATGTAGAAGGGGAGAAATTCTGCGGACATGTGGGGAAATCCAAAAAACAGGCAGAGTTGAGTGCCGCAAAGGCTGCTTACACCGCTTTCGAAGAGCGTGCTCTTAAAACCACTCAAAGCGGTAACTGGAGATCGAAAGCGATTCCTGAACAAGATTTGGTTCCTTCTCTAACTACCAAGAACAAGGAATGTGGTAAAGACACTAAAGGGATCGATGAACAAGAGTCGGACATGAGTCTCAGAGCTGTGTACTGTAAGGCACCTGATGCAATGGTACTGGAAATGGGAAATATGAACAAAACCGGAAAACCTTCCACATGCTTGGAACCAGTGGTCTCAGCATTTATGCATAACGATCTTTCGGCTGTCTTGAGTGCGGATGAAAATGAAGGGAAAATAGCGGGATTGAAGAGTTACTTACTATGCAACAGGTTTAGAGTCTACACACAGTTCCCAGATATTGCATTCCCAAAGTGCATAACTATGCTGCCTATAAGTGATGACAAATGGGTGGCTGTCAGGCTGGAGTTCCCAAACGAAGAAAGCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

343

Amino Acids

37.76

Weight (kDa)

5.25

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 3 - 66 4.4e-12 Double-stranded RNA binding motif
dsrm PF00035 124 - 186 1.3e-14 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016795)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27052
malus_domestica MD11G1178800.v1.1
prunus_persica Prupe.6G151600_v2.0.a1 Prupe.6G151600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0049631
rosa_laevigata RLG00000034628
rosa_multiflora Rmu_sc0013777.1_g000008
rosa_roxburghii Rroxscaffold_1G00032040
rosa_rugosa Rorug05G0249300
rosa_samantha Rh5AG325800 Rh5BG337100 Rh5CG361800 Rh5DG349300
rosa_wichuraiana Rw5G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 736
AccI GTMKAC 1 cut(s) 918
AciI CCGC 7 cut(s) 183, 501, 543, 561, 597, 854, 878
AclWI GGATC 1 cut(s) 695
AcsI RAATTY 1 cut(s) 494
AcuI CTGAAG 2 cut(s) 85, 288
AfaI GTAC 4 cut(s) 8, 277, 728, 753
AflIII ACRYGT 1 cut(s) 505
AgsI TTSAA 1 cut(s) 886
AjnI CCWGG 2 cut(s) 177, 358
AjuI GAANNNNNNNTTGG 2 cut(s) 451, 483
AluBI AGCT 4 cut(s) 28, 170, 395, 722
AluI AGCT 4 cut(s) 28, 170, 395, 722
Alw21I GWGCWC 1 cut(s) 580
Alw26I GTCTC 2 cut(s) 719, 817
AlwI GGATC 1 cut(s) 695
AlwNI CAGNNNCTG 2 cut(s) 28, 311
AoxI GGCC 1 cut(s) 180
ApeKI GCWGC 4 cut(s) 299, 311, 551, 964
ApoI RAATTY 1 cut(s) 494
Asp700I GAANNNNTTC 2 cut(s) 342, 787
AspS9I GGNCC 1 cut(s) 80
AsuHPI GGTGA 3 cut(s) 106, 199, 219
AsuII TTCGAA 1 cut(s) 567
AvaII GGWCC 1 cut(s) 80
BanI GGYRCC 1 cut(s) 736
BanII GRGCYC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 580
BbvI GCAGC 4 cut(s) 286, 298, 538, 951
BccI CCATC 1 cut(s) 71
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BciT130I CCWGG 2 cut(s) 179, 360
BcoDI GTCTC 2 cut(s) 719, 817
BfmI CTRYAG 1 cut(s) 20
BglI GCCNNNNNGGC 1 cut(s) 548
BisI GCNGC 6 cut(s) 183, 300, 312, 543, 552, 965
BlsI GCNGC 6 cut(s) 184, 301, 313, 544, 553, 966
BmcAI AGTACT 1 cut(s) 277
Bme1390I CCNGG 2 cut(s) 179, 360
Bme18I GGWCC 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 80
BmiI GGNNCC 3 cut(s) 639, 738, 804
BmrFI CCNGG 2 cut(s) 179, 360
BmrI ACTGGG 2 cut(s) 137, 448
BmsI GCATC 2 cut(s) 455, 733
BmuI ACTGGG 2 cut(s) 137, 448
BoxI GACNNNNGTC 1 cut(s) 711
BpmI CTGGAG 2 cut(s) 625, 1022
Bpu14I TTCGAA 1 cut(s) 567
BpuEI CTTGAG 1 cut(s) 868
Bsa29I ATCGAT 1 cut(s) 690
BsaBI GATNNNNATC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 817
BsaJI CCNNGG 2 cut(s) 186, 249
BsaWI WCCGGW 1 cut(s) 779
Bse1I ACTGG 5 cut(s) 143, 443, 608, 759, 806
Bse3DI GCAATG 2 cut(s) 241, 753
Bse8I GATNNNNATC 1 cut(s) 90
BseBI CCWGG 2 cut(s) 179, 360
BseCI ATCGAT 1 cut(s) 690
BseDI CCNNGG 2 cut(s) 186, 249
BseGI GGATG 4 cut(s) 241, 355, 484, 862
BseJI GATNNNNATC 1 cut(s) 90
BseMI GCAATG 2 cut(s) 241, 753
BseMII CTCAG 3 cut(s) 410, 730, 828
BseNI ACTGG 5 cut(s) 143, 443, 608, 759, 806
BseXI GCAGC 4 cut(s) 286, 298, 538, 951
BshFI GGCC 1 cut(s) 182
BshNI GGYRCC 1 cut(s) 736
BshVI ATCGAT 1 cut(s) 690
BsiHKAI GWGCWC 1 cut(s) 580
BsiSI CCGG 1 cut(s) 780
BsmAI GTCTC 2 cut(s) 719, 817
BsmI GAATGC 2 cut(s) 301, 941
BsnI GGCC 1 cut(s) 182
Bso31I GGTCTC 1 cut(s) 817
Bsp119I TTCGAA 1 cut(s) 567
Bsp1286I GDGCHC 2 cut(s) 260, 580
Bsp1407I TGTACA 1 cut(s) 6
Bsp143I GATC 4 cut(s) 85, 608, 687, 832
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 7 cut(s) 183, 501, 543, 561, 597, 854, 878
BspANI GGCC 1 cut(s) 182
BspCNI CTCAG 3 cut(s) 409, 729, 827
BspDI ATCGAT 1 cut(s) 690
BspLI GGNNCC 3 cut(s) 639, 738, 804
BspMAI CTGCAG 1 cut(s) 24
BspPI GGATC 1 cut(s) 695
BspQI GCTCTTC 1 cut(s) 564
BspT104I TTCGAA 1 cut(s) 567
BspT107I GGYRCC 1 cut(s) 736
BspTNI GGTCTC 1 cut(s) 817
BsrDI GCAATG 2 cut(s) 241, 753
BsrGI TGTACA 1 cut(s) 6
BsrI ACTGG 5 cut(s) 143, 443, 608, 759, 806
BssECI CCNNGG 2 cut(s) 186, 249
BssMI GATC 4 cut(s) 85, 608, 687, 832
BssT1I CCWWGG 2 cut(s) 186, 249
Bst2UI CCWGG 2 cut(s) 179, 360
Bst4CI ACNGT 5 cut(s) 118, 433, 475, 731, 927
Bst6I CTCTTC 3 cut(s) 472, 564, 881
BstAPI GCANNNNNTGC 3 cut(s) 296, 551, 743
BstAUI TGTACA 1 cut(s) 6
BstBI TTCGAA 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 420, 576
BstDEI CTNAG 3 cut(s) 396, 716, 814
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 4 cut(s) 241, 355, 484, 862
BstKTI GATC 4 cut(s) 88, 611, 690, 835
BstMAI GTCTC 2 cut(s) 719, 817
BstMBI GATC 4 cut(s) 85, 608, 687, 832
BstMWI GCNNNNNNNGC 7 cut(s) 191, 296, 308, 548, 551, 560, 743
BstNI CCWGG 2 cut(s) 179, 360
BstNSI RCATGY 3 cut(s) 457, 509, 798
BstPAI GACNNNNGTC 1 cut(s) 711
BstSCI CCNGG 2 cut(s) 177, 358
BstSFI CTRYAG 1 cut(s) 20
BstV1I GCAGC 4 cut(s) 286, 298, 538, 951
BstXI CCANNNNNNTGG 1 cut(s) 799
Bsu15I ATCGAT 1 cut(s) 690
BsuRI GGCC 1 cut(s) 182
BsuTUI ATCGAT 1 cut(s) 690
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 4 cut(s) 241, 355, 484, 862
BtsI GCAGTG 1 cut(s) 413
BtsIMutI CAGTG 3 cut(s) 413, 471, 813
Cac8I GCNNGC 2 cut(s) 420, 576
CaiI CAGNNNCTG 2 cut(s) 28, 311
Cfr13I GGNCC 1 cut(s) 80
ClaI ATCGAT 1 cut(s) 690
CsiI ACCWGGT 1 cut(s) 358
Csp6I GTAC 4 cut(s) 7, 276, 727, 752
CspCI CAANNNNNGTGG 2 cut(s) 649, 684
CviAII CATG 7 cut(s) 35, 70, 187, 454, 506, 709, 795
CviQI GTAC 4 cut(s) 7, 276, 727, 752
DdeI CTNAG 3 cut(s) 396, 716, 814
DpnI GATC 4 cut(s) 87, 610, 689, 834
DpnII GATC 4 cut(s) 85, 608, 687, 832
Eam1104I CTCTTC 3 cut(s) 472, 564, 881
EarI CTCTTC 3 cut(s) 472, 564, 881
Eco130I CCWWGG 2 cut(s) 186, 249
Eco24I GRGCYC 1 cut(s) 260
Eco31I GGTCTC 1 cut(s) 817
Eco47I GGWCC 1 cut(s) 80
Eco57I CTGAAG 2 cut(s) 85, 288
EcoRII CCWGG 2 cut(s) 177, 358
EcoT14I CCWWGG 2 cut(s) 186, 249
EcoT22I ATGCAT 1 cut(s) 828
EcoT38I GRGCYC 1 cut(s) 260
ErhI CCWWGG 2 cut(s) 186, 249
FaeI CATG 7 cut(s) 38, 73, 190, 457, 509, 712, 798
FalI AAGNNNNNCTT 4 cut(s) 365, 397, 878, 910
FatI CATG 7 cut(s) 34, 69, 186, 453, 505, 708, 794
FauI CCCGC 1 cut(s) 871
FblI GTMKAC 1 cut(s) 918
Fnu4HI GCNGC 6 cut(s) 183, 300, 312, 543, 552, 965
FokI GGATG 4 cut(s) 248, 362, 491, 869
FriOI GRGCYC 1 cut(s) 260
Fsp4HI GCNGC 6 cut(s) 183, 300, 312, 543, 552, 965
GluI GCNGC 6 cut(s) 183, 300, 312, 543, 552, 965
GsuI CTGGAG 2 cut(s) 625, 1022
HaeIII GGCC 1 cut(s) 182
HapII CCGG 1 cut(s) 780
Hin1II CATG 7 cut(s) 38, 73, 190, 457, 509, 712, 798
HinfI GANTC 6 cut(s) 137, 376, 619, 701, 712, 915
HpaII CCGG 1 cut(s) 780
HphI GGTGA 3 cut(s) 106, 199, 219
Hpy166II GTNNAC 3 cut(s) 436, 727, 919
Hpy188I TCNGA 5 cut(s) 307, 337, 399, 706, 719
Hpy188III TCNNGA 3 cut(s) 347, 623, 847
Hpy8I GTNNAC 3 cut(s) 436, 727, 919
HpyAV CCTTC 7 cut(s) 228, 332, 398, 479, 651, 798, 860
HpyCH4III ACNGT 5 cut(s) 118, 433, 475, 731, 927
HpyCH4V TGCA 8 cut(s) 22, 246, 446, 746, 826, 903, 941, 954
HpyF10VI GCNNNNNNNGC 7 cut(s) 191, 296, 308, 548, 551, 560, 743
HpyF3I CTNAG 3 cut(s) 396, 716, 814
Hsp92II CATG 7 cut(s) 38, 73, 190, 457, 509, 712, 798
Kzo9I GATC 4 cut(s) 85, 608, 687, 832
LguI GCTCTTC 1 cut(s) 564
LmnI GCTCC 1 cut(s) 25
Lsp1109I GCAGC 4 cut(s) 286, 298, 538, 951
LweI GCATC 2 cut(s) 455, 733
MabI ACCWGGT 1 cut(s) 358
MaeIII GTNAC 3 cut(s) 112, 599, 890
MalI GATC 4 cut(s) 87, 610, 689, 834
MboI GATC 4 cut(s) 85, 608, 687, 832
MboII GAAGA 8 cut(s) 150, 191, 213, 216, 459, 581, 898, 1028
MhlI GDGCHC 2 cut(s) 260, 580
MluCI AATT 1 cut(s) 494
MlyI GAGTC 4 cut(s) 131, 710, 721, 924
MmeI TCCRAC 1 cut(s) 684
MnlI CCTC 2 cut(s) 220, 301
Mph1103I ATGCAT 1 cut(s) 828
MroXI GAANNNNTTC 2 cut(s) 342, 787
MseI TTAA 1 cut(s) 582
MslI CAYNNNNRTG 2 cut(s) 68, 959
MspI CCGG 1 cut(s) 780
MspR9I CCNGG 2 cut(s) 179, 360
Mva1269I GAATGC 2 cut(s) 301, 941
MvaI CCWGG 2 cut(s) 179, 360
MwoI GCNNNNNNNGC 7 cut(s) 191, 296, 308, 548, 551, 560, 743
NcoI CCATGG 1 cut(s) 186
NdeII GATC 4 cut(s) 85, 608, 687, 832
NlaIII CATG 7 cut(s) 38, 73, 190, 457, 509, 712, 798
NlaIV GGNNCC 3 cut(s) 639, 738, 804
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 112
NsiI ATGCAT 1 cut(s) 828
NspI RCATGY 3 cut(s) 457, 509, 798
NspV TTCGAA 1 cut(s) 567
PciI ACATGT 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 564
PcsI WCGNNNNNNNCGW 1 cut(s) 1020
PctI GAATGC 2 cut(s) 301, 941
PdmI GAANNNNTTC 2 cut(s) 342, 787
PfeI GAWTC 2 cut(s) 376, 619
PkrI GCNGC 6 cut(s) 184, 301, 313, 544, 553, 966
PleI GAGTC 4 cut(s) 131, 709, 720, 923
PpsI GAGTC 4 cut(s) 131, 709, 720, 923
PscI ACATGT 1 cut(s) 505
PshAI GACNNNNGTC 1 cut(s) 711
Psp6I CCWGG 2 cut(s) 177, 358
PspGI CCWGG 2 cut(s) 177, 358
PspN4I GGNNCC 3 cut(s) 639, 738, 804
PspPI GGNCC 1 cut(s) 80
PstI CTGCAG 1 cut(s) 24
PstNI CAGNNNCTG 2 cut(s) 28, 311
RsaI GTAC 4 cut(s) 8, 277, 728, 753
RsaNI GTAC 4 cut(s) 7, 276, 727, 752
RseI CAYNNNNRTG 2 cut(s) 68, 959
SapI GCTCTTC 1 cut(s) 564
SaqAI TTAA 1 cut(s) 582
SatI GCNGC 6 cut(s) 183, 300, 312, 543, 552, 965
Sau3AI GATC 4 cut(s) 85, 608, 687, 832
Sau96I GGNCC 1 cut(s) 80
ScaI AGTACT 1 cut(s) 277
SchI GAGTC 4 cut(s) 131, 710, 721, 924
ScrFI CCNGG 2 cut(s) 179, 360
SduI GDGCHC 2 cut(s) 260, 580
SexAI ACCWGGT 1 cut(s) 358
SfaNI GCATC 2 cut(s) 455, 733
SfcI CTRYAG 1 cut(s) 20
SfuI TTCGAA 1 cut(s) 567
SinI GGWCC 1 cut(s) 80
SmiMI CAYNNNNRTG 2 cut(s) 68, 959
SmlI CTYRAG 1 cut(s) 847
SmoI CTYRAG 1 cut(s) 847
Sse9I AATT 1 cut(s) 494
SsiI CCGC 7 cut(s) 183, 501, 543, 561, 597, 854, 878
StyD4I CCNGG 2 cut(s) 177, 358
StyI CCWWGG 2 cut(s) 186, 249
TaaI ACNGT 5 cut(s) 118, 433, 475, 731, 927
TaqI TCGA 3 cut(s) 567, 611, 690
TasI AATT 1 cut(s) 494
TatI WGTACW 3 cut(s) 6, 275, 726
TauI GCSGC 2 cut(s) 185, 545
TfiI GAWTC 2 cut(s) 376, 619
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 3 cut(s) 420, 478, 813
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 4 cut(s) 299, 311, 551, 964
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 9 cut(s) 51, 86, 252, 256, 318, 708, 785, 873, 879
TspGWI ACGGA 1 cut(s) 299
TspRI CASTG 3 cut(s) 420, 478, 813
VpaK11BI GGWCC 1 cut(s) 80
XapI RAATTY 1 cut(s) 494
XceI RCATGY 3 cut(s) 457, 509, 798
XmiI GTMKAC 1 cut(s) 918
XmnI GAANNNNTTC 2 cut(s) 342, 787
ZrmI AGTACT 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.