MD11G1178800.v1.1

Double-stranded RNA binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016795
Physical Location & Seq
Reverse (-)
21483419 .. 21491244
7826 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1178800.v1.1.491

Sequence Viewer

Length: 996 bp
ATGTACAAGAGTAAGCTGCAGGAGGTCTGCCATGGGAAGCAATGGGGGTTGCCAACATACACGGCCATGAAAGACGGACCGGATCACAACCCTTGCTTCAGGGCCTCTGTTTCGGTCAATGGCTTCTCCTTCGATTCCCCGGTTGCTTGCAAATCGTCCAAACAAGCCCAAAACCAAGCTGCCATGCTCGCTTTCTTTCACTTCACCTCTCCACCTTCTTCTAACCTTTCTTCTGGCGCTGCTGATCCAGAGGTGTACAACACTTTGAAGGAGGCAGAGCGCGCAGCTGCAAACACTGTTTTGATGTCACTGTCAGAAGACAGCTCCCGAATTAATGATGAGATGGGACATTACAAGAATCTTTTGCAGGAGTTAGCTCGGGAAGAAGGCTTTTGCATGCCGACATATAAAACTGTGAAATCCGGTGCATCTCATATGCCAACATTCTCTTCCACTGTGGAGGTAGAAGGTGAGCAGTTCTGCGGGAAAACAGGGAAATCCAAGAAACAGGCGGAGCTGAGTGCTGCTAAGGTTGCCTATATTTCGTTAAAAAAGCAAAGTGCCATCAAAACCACTCAAAGCTCAGACTTGACCATTGCTGTTGAGCCACTTGAGAATCTCATACACGAACATCAATCGGTTTTATCTCCAGCTATCAAGTATGAGGACCGTGCTAACGAAACTAAAGAAGTGCTAATGGATAACATGAACAAAACCGGACAGTCTTCCTCATGTTCTGAATCGATATTCCCTTCAACTGAAGAAAGCGGTCCCTCTCAAACATTTAAAAAACTGAAACCTCCTGCTGATGTTTCAATTTCCGATACAAATGGATGGAAAGGTGGTGGACTGAAGAGTTACTTGCTGTGCCACAGGGTTAGAGTTTACAACCAATTTCCAGATATTTCACTCCCTAATCACATAACGGTGCTGCCCATTAGTGATGACAAGTGGGTTCCCTTCATCCTGGAGTTCCCAAATGAAGAAAGCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

332

Amino Acids

36.42

Weight (kDa)

6.02

Isoelectric Point (pI)

55.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 2 - 64 2.5e-11 Double-stranded RNA binding motif
dsrm PF00035 118 - 180 3.9e-14 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016795)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27052
malus_domestica MD11G1178800.v1.1
prunus_persica Prupe.6G151600_v2.0.a1 Prupe.6G151600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0049631
rosa_laevigata RLG00000034628
rosa_multiflora Rmu_sc0013777.1_g000008
rosa_roxburghii Rroxscaffold_1G00032040
rosa_rugosa Rorug05G0249300
rosa_samantha Rh5AG325800 Rh5BG337100 Rh5CG361800 Rh5DG349300
rosa_wichuraiana Rw5G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 282
AciI CCGC 3 cut(s) 483, 512, 768
AclWI GGATC 2 cut(s) 90, 239
AcoI YGGCCR 1 cut(s) 63
AcuI CTGAAG 3 cut(s) 82, 780, 872
AfaI GTAC 2 cut(s) 5, 257
AgsI TTSAA 3 cut(s) 268, 756, 816
AjnI CCWGG 1 cut(s) 966
AjuI GAANNNNNNNTTGG 2 cut(s) 433, 465
AluBI AGCT 8 cut(s) 16, 179, 287, 324, 377, 517, 582, 653
AluI AGCT 8 cut(s) 16, 179, 287, 324, 377, 517, 582, 653
AlwI GGATC 2 cut(s) 90, 239
Ama87I CYCGRG 1 cut(s) 378
AoxI GGCC 2 cut(s) 63, 102
ApeKI GCWGC 7 cut(s) 16, 179, 239, 284, 287, 524, 931
AseI ATTAAT 1 cut(s) 333
AspLEI GCGC 3 cut(s) 239, 282, 284
AspS9I GGNCC 4 cut(s) 77, 102, 667, 770
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 2 cut(s) 196, 482
AvaI CYCGRG 1 cut(s) 378
AvaII GGWCC 3 cut(s) 77, 667, 770
BbsI GAAGAC 2 cut(s) 324, 717
BbvI GCAGC 7 cut(s) 3, 166, 226, 274, 296, 511, 918
BccI CCATC 3 cut(s) 337, 572, 828
BceAI ACGGC 1 cut(s) 78
BciT130I CCWGG 1 cut(s) 968
BcnI CCSGG 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 17
BfoI RGCGCY 1 cut(s) 240
BisI GCNGC 7 cut(s) 17, 180, 240, 285, 288, 525, 932
BlsI GCNGC 7 cut(s) 18, 181, 241, 286, 289, 526, 933
Bme1390I CCNGG 2 cut(s) 140, 968
Bme18I GGWCC 3 cut(s) 77, 667, 770
BmeT110I CYCGRG 1 cut(s) 378
BmgT120I GGNCC 4 cut(s) 77, 102, 667, 770
BmiI GGNNCC 2 cut(s) 772, 957
BmrFI CCNGG 2 cut(s) 140, 968
BmsI GCATC 1 cut(s) 437
BpiI GAAGAC 2 cut(s) 324, 717
BpmI CTGGAG 2 cut(s) 633, 989
Bpu10I CCTNAGC 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 1 cut(s) 140
Bsa29I ATCGAT 1 cut(s) 743
BsaJI CCNNGG 2 cut(s) 31, 138
BsaWI WCCGGW 3 cut(s) 79, 422, 716
Bse3DI GCAATG 2 cut(s) 47, 594
BseBI CCWGG 1 cut(s) 968
BseCI ATCGAT 1 cut(s) 743
BseDI CCNNGG 2 cut(s) 31, 138
BseGI GGATG 2 cut(s) 839, 963
BseMI GCAATG 2 cut(s) 47, 594
BseMII CTCAG 2 cut(s) 509, 597
BsePI GCGCGC 1 cut(s) 280
BseXI GCAGC 7 cut(s) 3, 166, 226, 274, 296, 511, 918
Bsh1236I CGCG 1 cut(s) 282
BshFI GGCC 2 cut(s) 65, 104
BshVI ATCGAT 1 cut(s) 743
BsiHKCI CYCGRG 1 cut(s) 378
BsiSI CCGG 4 cut(s) 80, 140, 423, 717
BslFI GGGAC 2 cut(s) 360, 756
BsmFI GGGAC 2 cut(s) 360, 756
BsnI GGCC 2 cut(s) 65, 104
BsoBI CYCGRG 1 cut(s) 378
Bsp1407I TGTACA 2 cut(s) 3, 255
Bsp143I GATC 2 cut(s) 82, 244
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 3 cut(s) 483, 512, 768
BspANI GGCC 2 cut(s) 65, 104
BspCNI CTCAG 2 cut(s) 510, 596
BspDI ATCGAT 1 cut(s) 743
BspFNI CGCG 1 cut(s) 282
BspLI GGNNCC 2 cut(s) 772, 957
BspMAI CTGCAG 1 cut(s) 21
BspPI GGATC 2 cut(s) 90, 239
BsrDI GCAATG 2 cut(s) 47, 594
BsrGI TGTACA 2 cut(s) 3, 255
BssECI CCNNGG 2 cut(s) 31, 138
BssHII GCGCGC 1 cut(s) 280
BssMI GATC 2 cut(s) 82, 244
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 968
Bst4CI ACNGT 7 cut(s) 298, 312, 415, 457, 671, 723, 928
Bst6I CTCTTC 2 cut(s) 454, 848
BstAUI TGTACA 2 cut(s) 3, 255
BstC8I GCNNGC 4 cut(s) 148, 189, 282, 398
BstDEI CTNAG 3 cut(s) 518, 528, 583
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 2 cut(s) 839, 963
BstFNI CGCG 1 cut(s) 282
BstH2I RGCGCY 1 cut(s) 240
BstHHI GCGC 3 cut(s) 239, 282, 284
BstKTI GATC 2 cut(s) 85, 247
BstMBI GATC 2 cut(s) 82, 244
BstMWI GCNNNNNNNGC 3 cut(s) 188, 281, 533
BstNI CCWGG 1 cut(s) 968
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 2 cut(s) 138, 966
BstSFI CTRYAG 1 cut(s) 17
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 7 cut(s) 3, 166, 226, 274, 296, 511, 918
BstV2I GAAGAC 2 cut(s) 324, 717
Bsu15I ATCGAT 1 cut(s) 743
BsuRI GGCC 2 cut(s) 65, 104
BsuTUI ATCGAT 1 cut(s) 743
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 2 cut(s) 839, 963
BtsIMutI CAGTG 3 cut(s) 294, 308, 453
Cac8I GCNNGC 4 cut(s) 148, 189, 282, 398
CfoI GCGC 3 cut(s) 239, 282, 284
Cfr13I GGNCC 4 cut(s) 77, 102, 667, 770
ClaI ATCGAT 1 cut(s) 743
CpoI CGGWCCG 1 cut(s) 77
Csp6I GTAC 2 cut(s) 4, 256
CspI CGGWCCG 1 cut(s) 77
CviAII CATG 6 cut(s) 32, 67, 184, 397, 706, 732
CviQI GTAC 2 cut(s) 4, 256
DdeI CTNAG 3 cut(s) 518, 528, 583
DpnI GATC 2 cut(s) 84, 246
DpnII GATC 2 cut(s) 82, 244
DraI TTTAAA 1 cut(s) 787
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 2 cut(s) 454, 848
EarI CTCTTC 2 cut(s) 454, 848
EciI GGCGGA 1 cut(s) 527
Eco130I CCWWGG 1 cut(s) 31
Eco47I GGWCC 3 cut(s) 77, 667, 770
Eco57I CTGAAG 3 cut(s) 82, 780, 872
Eco88I CYCGRG 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 966
EcoT14I CCWWGG 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 6 cut(s) 35, 70, 187, 400, 709, 735
FalI AAGNNNNNCTT 2 cut(s) 845, 877
FaqI GGGAC 2 cut(s) 360, 756
FatI CATG 6 cut(s) 31, 66, 183, 396, 705, 731
FauI CCCGC 1 cut(s) 476
FauNDI CATATG 1 cut(s) 435
Fnu4HI GCNGC 7 cut(s) 17, 180, 240, 285, 288, 525, 932
FokI GGATG 2 cut(s) 846, 950
Fsp4HI GCNGC 7 cut(s) 17, 180, 240, 285, 288, 525, 932
GlaI GCGC 3 cut(s) 238, 281, 283
GluI GCNGC 7 cut(s) 17, 180, 240, 285, 288, 525, 932
GsuI CTGGAG 2 cut(s) 633, 989
HaeII RGCGCY 1 cut(s) 240
HaeIII GGCC 2 cut(s) 65, 104
HapII CCGG 4 cut(s) 80, 140, 423, 717
HhaI GCGC 3 cut(s) 239, 282, 284
Hin1II CATG 6 cut(s) 35, 70, 187, 400, 709, 735
Hin6I GCGC 3 cut(s) 237, 280, 282
HinP1I GCGC 3 cut(s) 237, 280, 282
HinfI GANTC 4 cut(s) 134, 358, 616, 740
HpaII CCGG 4 cut(s) 80, 140, 423, 717
HphI GGTGA 2 cut(s) 196, 482
Hpy166II GTNNAC 3 cut(s) 256, 848, 886
Hpy188I TCNGA 4 cut(s) 316, 586, 739, 823
Hpy188III TCNNGA 4 cut(s) 248, 327, 380, 899
Hpy8I GTNNAC 3 cut(s) 256, 848, 886
HpyAV CCTTC 7 cut(s) 139, 225, 262, 380, 461, 762, 970
HpyCH4III ACNGT 7 cut(s) 298, 312, 415, 457, 671, 723, 928
HpyCH4V TGCA 6 cut(s) 19, 150, 290, 367, 396, 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 281, 533
HpyF3I CTNAG 3 cut(s) 518, 528, 583
Hsp92II CATG 6 cut(s) 35, 70, 187, 400, 709, 735
HspAI GCGC 3 cut(s) 237, 280, 282
Kzo9I GATC 2 cut(s) 82, 244
LmnI GCTCC 2 cut(s) 329, 514
Lsp1109I GCAGC 7 cut(s) 3, 166, 226, 274, 296, 511, 918
LweI GCATC 1 cut(s) 437
MaeIII GTNAC 2 cut(s) 306, 857
MalI GATC 2 cut(s) 84, 246
MboI GATC 2 cut(s) 82, 244
MboII GAAGA 9 cut(s) 210, 222, 329, 395, 441, 717, 773, 865, 995
MfeI CAATTG 1 cut(s) 991
MluCI AATT 4 cut(s) 330, 816, 893, 991
MseI TTAA 3 cut(s) 333, 548, 786
MslI CAYNNNNRTG 2 cut(s) 65, 926
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 4 cut(s) 80, 140, 423, 717
MspR9I CCNGG 2 cut(s) 140, 968
MunI CAATTG 1 cut(s) 991
MvaI CCWGG 1 cut(s) 968
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 3 cut(s) 188, 281, 533
NciI CCSGG 1 cut(s) 140
NcoI CCATGG 1 cut(s) 31
NdeI CATATG 1 cut(s) 435
NdeII GATC 2 cut(s) 82, 244
NlaIII CATG 6 cut(s) 35, 70, 187, 400, 709, 735
NlaIV GGNNCC 2 cut(s) 772, 957
NmuCI GTSAC 1 cut(s) 306
NspI RCATGY 1 cut(s) 400
PaeI GCATGC 1 cut(s) 400
PauI GCGCGC 1 cut(s) 280
PfeI GAWTC 4 cut(s) 134, 358, 616, 740
PfoI TCCNGGA 1 cut(s) 966
PkrI GCNGC 7 cut(s) 18, 181, 241, 286, 289, 526, 933
PshBI ATTAAT 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 966
PspGI CCWGG 1 cut(s) 966
PspN4I GGNNCC 2 cut(s) 772, 957
PspPI GGNCC 4 cut(s) 77, 102, 667, 770
PstI CTGCAG 1 cut(s) 21
PteI GCGCGC 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 2 cut(s) 5, 257
RsaNI GTAC 2 cut(s) 4, 256
RseI CAYNNNNRTG 2 cut(s) 65, 926
Rsr2I CGGWCCG 1 cut(s) 77
RsrII CGGWCCG 1 cut(s) 77
SaqAI TTAA 3 cut(s) 333, 548, 786
SatI GCNGC 7 cut(s) 17, 180, 240, 285, 288, 525, 932
Sau3AI GATC 2 cut(s) 82, 244
Sau96I GGNCC 4 cut(s) 77, 102, 667, 770
ScrFI CCNGG 2 cut(s) 140, 968
SfaNI GCATC 1 cut(s) 437
SfcI CTRYAG 1 cut(s) 17
SinI GGWCC 3 cut(s) 77, 667, 770
SmiMI CAYNNNNRTG 2 cut(s) 65, 926
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
SphI GCATGC 1 cut(s) 400
Sse9I AATT 4 cut(s) 330, 816, 893, 991
SsiI CCGC 3 cut(s) 483, 512, 768
StyD4I CCNGG 2 cut(s) 138, 966
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 7 cut(s) 298, 312, 415, 457, 671, 723, 928
TaqI TCGA 2 cut(s) 132, 743
TaqII GACCGA 1 cut(s) 103
TasI AATT 4 cut(s) 330, 816, 893, 991
TatI WGTACW 2 cut(s) 3, 255
TfiI GAWTC 4 cut(s) 134, 358, 616, 740
Tru1I TTAA 3 cut(s) 333, 548, 786
Tru9I TTAA 3 cut(s) 333, 548, 786
TscAI CASTG 3 cut(s) 301, 315, 460
TseFI GTSAC 1 cut(s) 306
TseI GCWGC 7 cut(s) 16, 179, 239, 284, 287, 524, 931
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 4 cut(s) 83, 722, 952, 996
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 3 cut(s) 301, 315, 460
VpaK11BI GGWCC 3 cut(s) 77, 667, 770
VspI ATTAAT 1 cut(s) 333
XceI RCATGY 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.