RLG00000034628

Double-stranded RNA binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016795
Physical Location & Seq
Reverse (-)
46638186 .. 46640048
1863 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034628

Sequence Viewer

Length: 1107 bp
ATGTACAAGAGTAAGCTGCAGGAGCTGTGCCATGAAAAGAAGTGGGGTTTGCCAAGATACACTGCCATGAAAGATGGACCGGATCACAATCCTTGCTTCAAGGCTTGTGTCTCAGTACATGGCTACTCCTTTGACTCGCCAGTTACTTGTAAATCTTTAAAACAAGCCCAAAACCAAGCCGCCATGGTCGCTTTTCTTCACTTCACCTCTCCACCTTCTTCTTCTTCTTCTTCTTCACCGGTTCTTAAAGTTGGGTTTAAGGCTAGTACTGTTACAGTGGATGAGCATAGCACCTCGGAGAGCCCAGAGTTCAACACTAGTACTTTAAAGAAGGCAGAGAATGCTGCTTCTATGTCTATTTCATTCTCAGACGGGTTTCAGGATGGCGAACCTGGTCGTCTTTACAAGAATCTCTTACAGGAGTTGGCTCAGAGAGAAGGTTTTTCCATGCCAGCATATAAAACTGTGAGCTCGGGTGCATTTCACATGCCAACATTCTCTTCCACTGTGGATGTAGAAGGAGAGAGATTCTCCGGACATGTGGGGAAATCCAAAAAACAGGCAGAGTTGAGTGCTGCGAAGGTTGCTTACACTACCTTAAAAGAGCGTGCTCTCAAAACCACTCAAAGCACTGACTGGAGAAAGAATGTTGTTCCTGAGCAAGATTTGGTTTCTTCTCCAACTACCAAGAATAAGGAATGTGCTTTAGAAACTAAAGAGATCGATGAAGTTAAAGGATCAGACAATGTATCAGACAACGTCAATCTCAAAGCTGTGTACTGTGATGCACCTGATGCAATGGTACTGGAAGTCGATAATATGAATAAAACCGGAAAGCCTTCCTCATGCTTGGAACCAGTGCTCTCTTCAACTAAAGAATACCCTTCCTCTCCAGCATTTATGCATACGGATCTTTCGACTATCTCAATTGCGGATAAAAATGAAGGGAAATTAACGGGATTGGAAAGTTACTTGCTGTGCAACAGGTTTAGAGTCTACACACATTTTCCAGATATTGCATTCCCCAAGTGCATAACTGTGCTGCCCATCAGCGATGACAAATGGGTGGCTGTCAGGTTGGAGTTCCCAAATGAAGAAAGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

369

Amino Acids

40.51

Weight (kDa)

6.17

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 2 - 65 4.3e-11 Double-stranded RNA binding motif
dsrm PF00035 135 - 198 1.2e-13 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016795)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27052
malus_domestica MD11G1178800.v1.1
prunus_persica Prupe.6G151600_v2.0.a1 Prupe.6G151600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0049631
rosa_laevigata RLG00000034628
rosa_multiflora Rmu_sc0013777.1_g000008
rosa_roxburghii Rroxscaffold_1G00032040
rosa_rugosa Rorug05G0249300
rosa_samantha Rh5AG325800 Rh5BG337100 Rh5CG361800 Rh5DG349300
rosa_wichuraiana Rw5G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 996
AccIII TCCGGA 1 cut(s) 533
AciI CCGC 2 cut(s) 180, 932
AclWI GGATC 3 cut(s) 90, 745, 918
AfaI GTAC 6 cut(s) 5, 117, 268, 322, 779, 804
AflIII ACRYGT 1 cut(s) 538
AgeI ACCGGT 1 cut(s) 238
AgsI TTSAA 3 cut(s) 100, 313, 870
AhlI ACTAGT 1 cut(s) 317
AjnI CCWGG 1 cut(s) 391
AjuI GAANNNNNNNTTGG 2 cut(s) 484, 516
AluBI AGCT 4 cut(s) 16, 25, 471, 773
AluI AGCT 4 cut(s) 16, 25, 471, 773
Alw21I GWGCWC 3 cut(s) 473, 613, 864
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 3 cut(s) 90, 745, 918
AlwNI CAGNNNCTG 1 cut(s) 25
Ama87I CYCGRG 1 cut(s) 472
Aor13HI TCCGGA 1 cut(s) 533
ApeKI GCWGC 4 cut(s) 16, 344, 575, 1042
AsiGI ACCGGT 1 cut(s) 238
Asp700I GAANNNNTTC 1 cut(s) 838
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 2 cut(s) 196, 228
AvaI CYCGRG 1 cut(s) 472
AvaII GGWCC 1 cut(s) 77
BanII GRGCYC 2 cut(s) 305, 473
BarI GAAGNNNNNNTAC 2 cut(s) 572, 604
Bbv12I GWGCWC 3 cut(s) 473, 613, 864
BbvI GCAGC 4 cut(s) 3, 331, 562, 1029
BccI CCATC 3 cut(s) 68, 377, 1055
BciT130I CCWGG 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 115
BcuI ACTAGT 1 cut(s) 317
BfaI CTAG 2 cut(s) 264, 318
BfmI CTRYAG 1 cut(s) 17
BisI GCNGC 5 cut(s) 17, 180, 345, 576, 1043
BlsI GCNGC 5 cut(s) 18, 181, 346, 577, 1044
BmcAI AGTACT 2 cut(s) 268, 322
Bme1390I CCNGG 1 cut(s) 393
Bme18I GGWCC 1 cut(s) 77
BmeT110I CYCGRG 1 cut(s) 472
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 855
BmrFI CCNGG 1 cut(s) 393
BmsI GCATC 2 cut(s) 775, 784
BplI GAGNNNNNCTC 2 cut(s) 515, 547
BpmI CTGGAG 2 cut(s) 658, 876
Bpu10I CCTNAGC 1 cut(s) 657
Bsa29I ATCGAT 1 cut(s) 723
BsaBI GATNNNNATC 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 183, 294
BsaWI WCCGGW 4 cut(s) 79, 238, 533, 830
Bse118I RCCGGY 1 cut(s) 238
Bse1I ACTGG 4 cut(s) 140, 641, 810, 857
Bse3DI GCAATG 1 cut(s) 804
Bse8I GATNNNNATC 1 cut(s) 87
BseAI TCCGGA 1 cut(s) 533
BseBI CCWGG 1 cut(s) 393
BseCI ATCGAT 1 cut(s) 723
BseDI CCNNGG 2 cut(s) 183, 294
BseGI GGATG 3 cut(s) 286, 388, 517
BseJI GATNNNNATC 1 cut(s) 87
BseMI GCAATG 1 cut(s) 804
BseMII CTCAG 4 cut(s) 126, 381, 443, 648
BseNI ACTGG 4 cut(s) 140, 641, 810, 857
BseXI GCAGC 4 cut(s) 3, 331, 562, 1029
BshTI ACCGGT 1 cut(s) 238
BshVI ATCGAT 1 cut(s) 723
BsiHKAI GWGCWC 3 cut(s) 473, 613, 864
BsiHKCI CYCGRG 1 cut(s) 472
BsiSI CCGG 4 cut(s) 80, 239, 534, 831
BsmAI GTCTC 1 cut(s) 115
BsmI GAATGC 2 cut(s) 346, 1019
BsoBI CYCGRG 1 cut(s) 472
Bsp1286I GDGCHC 4 cut(s) 305, 473, 613, 864
Bsp13I TCCGGA 1 cut(s) 533
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 4 cut(s) 82, 720, 737, 910
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 2 cut(s) 180, 932
BspCNI CTCAG 4 cut(s) 125, 380, 442, 649
BspDI ATCGAT 1 cut(s) 723
BspEI TCCGGA 1 cut(s) 533
BspLI GGNNCC 1 cut(s) 855
BspMAI CTGCAG 1 cut(s) 21
BspPI GGATC 3 cut(s) 90, 745, 918
BsrDI GCAATG 1 cut(s) 804
BsrFI RCCGGY 1 cut(s) 238
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 4 cut(s) 140, 641, 810, 857
BssAI RCCGGY 1 cut(s) 238
BssECI CCNNGG 2 cut(s) 183, 294
BssMI GATC 4 cut(s) 82, 720, 737, 910
BssT1I CCWWGG 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 6 cut(s) 271, 277, 466, 508, 782, 1039
Bst6I CTCTTC 2 cut(s) 505, 871
BstAPI GCANNNNNTGC 2 cut(s) 341, 794
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 453, 609
BstDEI CTNAG 4 cut(s) 112, 367, 429, 657
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 3 cut(s) 286, 388, 517
BstKTI GATC 4 cut(s) 85, 723, 740, 913
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 4 cut(s) 82, 720, 737, 910
BstMWI GCNNNNNNNGC 5 cut(s) 22, 188, 341, 584, 794
BstNI CCWGG 1 cut(s) 393
BstNSI RCATGY 2 cut(s) 490, 542
BstSCI CCNGG 1 cut(s) 391
BstSFI CTRYAG 1 cut(s) 17
BstV1I GCAGC 4 cut(s) 3, 331, 562, 1029
BstX2I RGATCY 1 cut(s) 910
BstYI RGATCY 1 cut(s) 910
Bsu15I ATCGAT 1 cut(s) 723
BsuTUI ATCGAT 1 cut(s) 723
BtgI CCRYGG 1 cut(s) 183
BtgZI GCGATG 1 cut(s) 1068
BtsCI GGATG 3 cut(s) 286, 388, 517
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 5 cut(s) 60, 282, 504, 630, 864
Cac8I GCNNGC 2 cut(s) 453, 609
CaiI CAGNNNCTG 1 cut(s) 25
Cfr10I RCCGGY 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 77
ClaI ATCGAT 1 cut(s) 723
CsiI ACCWGGT 1 cut(s) 391
Csp6I GTAC 6 cut(s) 4, 116, 267, 321, 778, 803
CspAI ACCGGT 1 cut(s) 238
CviAII CATG 8 cut(s) 32, 67, 119, 184, 448, 487, 539, 846
CviQI GTAC 6 cut(s) 4, 116, 267, 321, 778, 803
DdeI CTNAG 4 cut(s) 112, 367, 429, 657
DpnI GATC 4 cut(s) 84, 722, 739, 912
DpnII GATC 4 cut(s) 82, 720, 737, 910
DraI TTTAAA 2 cut(s) 159, 327
Eam1104I CTCTTC 2 cut(s) 505, 871
EarI CTCTTC 2 cut(s) 505, 871
Ecl136II GAGCTC 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 183
Eco24I GRGCYC 2 cut(s) 305, 473
Eco47I GGWCC 1 cut(s) 77
Eco53kI GAGCTC 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 472
EcoICRI GAGCTC 1 cut(s) 471
EcoRII CCWGG 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 183
EcoT22I ATGCAT 1 cut(s) 906
EcoT38I GRGCYC 2 cut(s) 305, 473
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 8 cut(s) 35, 70, 122, 187, 451, 490, 542, 849
FalI AAGNNNNNCTT 2 cut(s) 398, 430
FatI CATG 8 cut(s) 31, 66, 118, 183, 447, 486, 538, 845
FblI GTMKAC 1 cut(s) 996
Fnu4HI GCNGC 5 cut(s) 17, 180, 345, 576, 1043
FokI GGATG 3 cut(s) 293, 395, 524
FriOI GRGCYC 2 cut(s) 305, 473
Fsp4HI GCNGC 5 cut(s) 17, 180, 345, 576, 1043
FspBI CTAG 2 cut(s) 264, 318
GluI GCNGC 5 cut(s) 17, 180, 345, 576, 1043
GsuI CTGGAG 2 cut(s) 658, 876
HapII CCGG 4 cut(s) 80, 239, 534, 831
Hin1II CATG 8 cut(s) 35, 70, 122, 187, 451, 490, 542, 849
HinfI GANTC 4 cut(s) 134, 409, 528, 993
HpaII CCGG 4 cut(s) 80, 239, 534, 831
HphI GGTGA 2 cut(s) 196, 228
Hpy166II GTNNAC 2 cut(s) 778, 997
Hpy188I TCNGA 5 cut(s) 298, 370, 432, 742, 754
Hpy188III TCNNGA 4 cut(s) 380, 534, 656, 1010
Hpy8I GTNNAC 2 cut(s) 778, 997
HpyAV CCTTC 8 cut(s) 225, 325, 431, 512, 574, 849, 894, 938
HpyCH4III ACNGT 6 cut(s) 271, 277, 466, 508, 782, 1039
HpyCH4IV ACGT 1 cut(s) 759
HpyCH4V TGCA 8 cut(s) 19, 479, 788, 797, 904, 981, 1019, 1032
HpyF10VI GCNNNNNNNGC 5 cut(s) 22, 188, 341, 584, 794
HpyF3I CTNAG 4 cut(s) 112, 367, 429, 657
HpySE526I ACGT 1 cut(s) 759
Hsp92II CATG 8 cut(s) 35, 70, 122, 187, 451, 490, 542, 849
Kpn2I TCCGGA 1 cut(s) 533
Kzo9I GATC 4 cut(s) 82, 720, 737, 910
LmnI GCTCC 1 cut(s) 22
Lsp1109I GCAGC 4 cut(s) 3, 331, 562, 1029
LweI GCATC 2 cut(s) 775, 784
MabI ACCWGGT 1 cut(s) 391
MaeI CTAG 2 cut(s) 264, 318
MaeII ACGT 1 cut(s) 759
MaeIII GTNAC 3 cut(s) 142, 271, 968
MalI GATC 4 cut(s) 84, 722, 739, 912
MboI GATC 4 cut(s) 82, 720, 737, 910
MfeI CAATTG 1 cut(s) 927
MflI RGATCY 1 cut(s) 910
MhlI GDGCHC 4 cut(s) 305, 473, 613, 864
MluCI AATT 3 cut(s) 927, 950, 1102
MlyI GAGTC 2 cut(s) 128, 1002
MmeI TCCRAC 2 cut(s) 704, 1059
MnlI CCTC 4 cut(s) 217, 304, 853, 898
Mph1103I ATGCAT 1 cut(s) 906
MroI TCCGGA 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 838
MseI TTAA 7 cut(s) 158, 246, 258, 326, 599, 732, 953
MslI CAYNNNNRTG 2 cut(s) 65, 1037
MspI CCGG 4 cut(s) 80, 239, 534, 831
MspR9I CCNGG 1 cut(s) 393
MunI CAATTG 1 cut(s) 927
Mva1269I GAATGC 2 cut(s) 346, 1019
MvaI CCWGG 1 cut(s) 393
MwoI GCNNNNNNNGC 5 cut(s) 22, 188, 341, 584, 794
NcoI CCATGG 1 cut(s) 183
NdeII GATC 4 cut(s) 82, 720, 737, 910
NlaIII CATG 8 cut(s) 35, 70, 122, 187, 451, 490, 542, 849
NlaIV GGNNCC 1 cut(s) 855
NsiI ATGCAT 1 cut(s) 906
NspI RCATGY 2 cut(s) 490, 542
PciI ACATGT 1 cut(s) 538
PcsI WCGNNNNNNNCGW 1 cut(s) 914
PctI GAATGC 2 cut(s) 346, 1019
PdmI GAANNNNTTC 1 cut(s) 838
PfeI GAWTC 2 cut(s) 409, 528
PflFI GACNNNGTC 1 cut(s) 758
PinAI ACCGGT 1 cut(s) 238
PkrI GCNGC 5 cut(s) 18, 181, 346, 577, 1044
PleI GAGTC 2 cut(s) 128, 1001
PpsI GAGTC 2 cut(s) 128, 1001
PscI ACATGT 1 cut(s) 538
Psp124BI GAGCTC 1 cut(s) 473
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 1 cut(s) 855
PspPI GGNCC 1 cut(s) 77
PstI CTGCAG 1 cut(s) 21
PstNI CAGNNNCTG 1 cut(s) 25
PsuI RGATCY 1 cut(s) 910
PsyI GACNNNGTC 1 cut(s) 758
RsaI GTAC 6 cut(s) 5, 117, 268, 322, 779, 804
RsaNI GTAC 6 cut(s) 4, 116, 267, 321, 778, 803
RseI CAYNNNNRTG 2 cut(s) 65, 1037
SacI GAGCTC 1 cut(s) 473
SaqAI TTAA 7 cut(s) 158, 246, 258, 326, 599, 732, 953
SatI GCNGC 5 cut(s) 17, 180, 345, 576, 1043
Sau3AI GATC 4 cut(s) 82, 720, 737, 910
Sau96I GGNCC 1 cut(s) 77
ScaI AGTACT 2 cut(s) 268, 322
SchI GAGTC 2 cut(s) 128, 1002
ScrFI CCNGG 1 cut(s) 393
SduI GDGCHC 4 cut(s) 305, 473, 613, 864
SexAI ACCWGGT 1 cut(s) 391
SfaNI GCATC 2 cut(s) 775, 784
SfcI CTRYAG 1 cut(s) 17
SinI GGWCC 1 cut(s) 77
SmiMI CAYNNNNRTG 2 cut(s) 65, 1037
SpeI ACTAGT 1 cut(s) 317
Sse9I AATT 3 cut(s) 927, 950, 1102
SsiI CCGC 2 cut(s) 180, 932
SspMI CTAG 2 cut(s) 264, 318
SstI GAGCTC 1 cut(s) 473
StyD4I CCNGG 1 cut(s) 391
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 6 cut(s) 271, 277, 466, 508, 782, 1039
TaiI ACGT 1 cut(s) 762
TaqI TCGA 3 cut(s) 723, 813, 917
TasI AATT 3 cut(s) 927, 950, 1102
TatI WGTACW 5 cut(s) 3, 115, 266, 320, 777
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 2 cut(s) 409, 528
Tru1I TTAA 7 cut(s) 158, 246, 258, 326, 599, 732, 953
Tru9I TTAA 7 cut(s) 158, 246, 258, 326, 599, 732, 953
TscAI CASTG 5 cut(s) 67, 282, 511, 637, 864
TseI GCWGC 4 cut(s) 16, 344, 575, 1042
TspDTI ATGAA 7 cut(s) 48, 83, 351, 741, 836, 957, 1107
TspGWI ACGGA 1 cut(s) 923
TspRI CASTG 5 cut(s) 67, 282, 511, 637, 864
Tth111I GACNNNGTC 1 cut(s) 758
VpaK11BI GGWCC 1 cut(s) 77
XceI RCATGY 2 cut(s) 490, 542
XmiI GTMKAC 1 cut(s) 996
XmnI GAANNNNTTC 1 cut(s) 838
XspI CTAG 2 cut(s) 264, 318
ZrmI AGTACT 2 cut(s) 268, 322
Zsp2I ATGCAT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.