Rw5G030790

Double-stranded RNA binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016795
Physical Location & Seq
Reverse (-)
49161866 .. 49163904
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030790.1

Sequence Viewer

Length: 867 bp
ATGGACCCTCTATTAGTTCTTGAAGTTGGGTTTAAGGCTAGTACTGTTACAGTGGATGAGCATAGCACCTCGGAGAGCCCAGAGTTCAACACTACTAGTACTTTAAAGAAGGCAGAGAATGCTGCTTCCATGTCTATTTCATTCTCAGACGGGTTTCAGGATGACGAACCTGGTCGTCTTTACAAGAATCTCTTACAGGAGTTGGCTCAGAGAGAAGGTTTTTCCATGCCAGCATATACAACTGTGAACTCGGGTGCATTTCACATGCCAACATTCTATTCCACTGTGGATGTGGAAGGAGAGAAATTCTCCGGACATGTGGGGAAATCCAAAAAACAGGCAGAGTTGAGTGCTGCGAAGGTTGCTTACACCACCTTAAAAGAGCGTGCTCTGAAAACCACTCAAAGCACTAACTGGAGAATGAATTTTGTTCCTGAACAAGATTTGGTTTCCTCTCCAACTACCAAGAATAAGGAATGTGCTTTAGAAACTAAAGGGTCAGACAATTCATTGGACAACATCAATCTCAAAGCTGTGTACTGTAATGCACCTGATGCAATGGTACTGGAAATCGATAATATGAATAAAACTGGAAAGCCTTCCTCATGCTTGGAACCAGTGCTCTCTTCAACTAAAGAATACCCTTCCTCTCCAGCATTTATGCATACTGATCTTTCGGCTATCTCAATTGCGGATAAAAATGAAGGGAAATTAACGGGATTGAAAAGTTACTTGCTGTGCAACAGGTTTAGAGTCTACACACATTTTCCAGATATTGCATTCCCCAAGTGCATAACTGTGCTGCCCATCAGTGATGACAAATGGGTGGCTGTCAGGTTGGAGTTCCCAAATGAAGAAAGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

288

Amino Acids

31.81

Weight (kDa)

5.27

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 61 - 124 1e-13 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016795)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27052
malus_domestica MD11G1178800.v1.1
prunus_persica Prupe.6G151600_v2.0.a1 Prupe.6G151600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0049631
rosa_laevigata RLG00000034628
rosa_multiflora Rmu_sc0013777.1_g000008
rosa_roxburghii Rroxscaffold_1G00032040
rosa_rugosa Rorug05G0249300
rosa_samantha Rh5AG325800 Rh5BG337100 Rh5CG361800 Rh5DG349300
rosa_wichuraiana Rw5G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 756
AccIII TCCGGA 1 cut(s) 311
AciI CCGC 1 cut(s) 692
AcsI RAATTY 2 cut(s) 305, 424
AfaI GTAC 4 cut(s) 43, 100, 539, 564
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 4 cut(s) 23, 88, 630, 724
AhlI ACTAGT 1 cut(s) 95
AjnI CCWGG 1 cut(s) 169
AjuI GAANNNNNNNTTGG 2 cut(s) 262, 294
AluBI AGCT 1 cut(s) 533
AluI AGCT 1 cut(s) 533
Alw21I GWGCWC 2 cut(s) 391, 624
Ama87I CYCGRG 1 cut(s) 250
Aor13HI TCCGGA 1 cut(s) 311
ApeKI GCWGC 3 cut(s) 122, 353, 802
ApoI RAATTY 2 cut(s) 305, 424
Asp700I GAANNNNTTC 1 cut(s) 598
AspS9I GGNCC 1 cut(s) 4
AvaI CYCGRG 1 cut(s) 250
AvaII GGWCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 80
BarI GAAGNNNNNNTAC 2 cut(s) 350, 382
Bbv12I GWGCWC 2 cut(s) 391, 624
BbvI GCAGC 3 cut(s) 109, 340, 789
BccI CCATC 1 cut(s) 815
BciT130I CCWGG 1 cut(s) 171
BcuI ACTAGT 1 cut(s) 95
BfaI CTAG 2 cut(s) 39, 96
BisI GCNGC 3 cut(s) 123, 354, 803
BlsI GCNGC 3 cut(s) 124, 355, 804
BmcAI AGTACT 2 cut(s) 43, 100
Bme1390I CCNGG 1 cut(s) 171
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 250
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 6, 615
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 1 cut(s) 544
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpmI CTGGAG 2 cut(s) 436, 636
Bsa29I ATCGAT 1 cut(s) 573
BsaJI CCNNGG 1 cut(s) 69
BsaWI WCCGGW 1 cut(s) 311
Bse1I ACTGG 4 cut(s) 419, 570, 595, 617
Bse3DI GCAATG 1 cut(s) 564
BseAI TCCGGA 1 cut(s) 311
BseBI CCWGG 1 cut(s) 171
BseCI ATCGAT 1 cut(s) 573
BseDI CCNNGG 1 cut(s) 69
BseGI GGATG 3 cut(s) 61, 166, 295
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 2 cut(s) 159, 221
BseNI ACTGG 4 cut(s) 419, 570, 595, 617
BseXI GCAGC 3 cut(s) 109, 340, 789
BshVI ATCGAT 1 cut(s) 573
BsiHKAI GWGCWC 2 cut(s) 391, 624
BsiHKCI CYCGRG 1 cut(s) 250
BsiSI CCGG 1 cut(s) 312
BsmI GAATGC 2 cut(s) 124, 779
BsoBI CYCGRG 1 cut(s) 250
Bsp1286I GDGCHC 3 cut(s) 80, 391, 624
Bsp13I TCCGGA 1 cut(s) 311
Bsp143I GATC 1 cut(s) 670
BspACI CCGC 1 cut(s) 692
BspCNI CTCAG 2 cut(s) 158, 220
BspDI ATCGAT 1 cut(s) 573
BspEI TCCGGA 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 6, 615
BsrDI GCAATG 1 cut(s) 564
BsrI ACTGG 4 cut(s) 419, 570, 595, 617
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 1 cut(s) 670
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 6 cut(s) 46, 52, 244, 286, 542, 799
Bst6I CTCTTC 1 cut(s) 631
BstAPI GCANNNNNTGC 2 cut(s) 119, 554
BstC8I GCNNGC 2 cut(s) 231, 387
BstDEI CTNAG 2 cut(s) 145, 207
BstF5I GGATG 3 cut(s) 61, 166, 295
BstKTI GATC 1 cut(s) 673
BstMBI GATC 1 cut(s) 670
BstMWI GCNNNNNNNGC 3 cut(s) 119, 362, 554
BstNI CCWGG 1 cut(s) 171
BstNSI RCATGY 2 cut(s) 268, 320
BstSCI CCNGG 1 cut(s) 169
BstV1I GCAGC 3 cut(s) 109, 340, 789
Bsu15I ATCGAT 1 cut(s) 573
BsuTUI ATCGAT 1 cut(s) 573
BtsCI GGATG 3 cut(s) 61, 166, 295
BtsIMutI CAGTG 4 cut(s) 57, 282, 624, 817
Cac8I GCNNGC 2 cut(s) 231, 387
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 1 cut(s) 573
CsiI ACCWGGT 1 cut(s) 169
Csp6I GTAC 4 cut(s) 42, 99, 538, 563
CviAII CATG 5 cut(s) 130, 226, 265, 317, 606
CviJI RGCY 7 cut(s) 38, 78, 206, 533, 598, 680, 830
CviKI_1 RGCY 7 cut(s) 38, 78, 206, 533, 598, 680, 830
CviQI GTAC 4 cut(s) 42, 99, 538, 563
DdeI CTNAG 2 cut(s) 145, 207
DpnI GATC 1 cut(s) 672
DpnII GATC 1 cut(s) 670
DraI TTTAAA 1 cut(s) 105
Eam1104I CTCTTC 1 cut(s) 631
EarI CTCTTC 1 cut(s) 631
Eco24I GRGCYC 1 cut(s) 80
Eco47I GGWCC 1 cut(s) 4
Eco88I CYCGRG 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 169
EcoT22I ATGCAT 1 cut(s) 666
EcoT38I GRGCYC 1 cut(s) 80
FaeI CATG 5 cut(s) 133, 229, 268, 320, 609
FalI AAGNNNNNCTT 2 cut(s) 176, 208
FatI CATG 5 cut(s) 129, 225, 264, 316, 605
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 3 cut(s) 123, 354, 803
FokI GGATG 3 cut(s) 68, 173, 302
FriOI GRGCYC 1 cut(s) 80
Fsp4HI GCNGC 3 cut(s) 123, 354, 803
FspBI CTAG 2 cut(s) 39, 96
GluI GCNGC 3 cut(s) 123, 354, 803
GsuI CTGGAG 2 cut(s) 436, 636
HapII CCGG 1 cut(s) 312
Hin1II CATG 5 cut(s) 133, 229, 268, 320, 609
HinfI GANTC 2 cut(s) 187, 753
HpaII CCGG 1 cut(s) 312
Hpy166II GTNNAC 3 cut(s) 247, 538, 757
Hpy188I TCNGA 5 cut(s) 73, 148, 210, 393, 502
Hpy188III TCNNGA 5 cut(s) 20, 158, 312, 434, 770
Hpy8I GTNNAC 3 cut(s) 247, 538, 757
HpyAV CCTTC 7 cut(s) 103, 209, 290, 352, 609, 654, 698
HpyCH4III ACNGT 6 cut(s) 46, 52, 244, 286, 542, 799
HpyCH4V TGCA 7 cut(s) 257, 548, 557, 664, 741, 779, 792
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 362, 554
HpyF3I CTNAG 2 cut(s) 145, 207
Hsp92II CATG 5 cut(s) 133, 229, 268, 320, 609
Kpn2I TCCGGA 1 cut(s) 311
Kzo9I GATC 1 cut(s) 670
Lsp1109I GCAGC 3 cut(s) 109, 340, 789
LweI GCATC 1 cut(s) 544
MabI ACCWGGT 1 cut(s) 169
MaeI CTAG 2 cut(s) 39, 96
MaeIII GTNAC 2 cut(s) 46, 728
MalI GATC 1 cut(s) 672
MboI GATC 1 cut(s) 670
MboII GAAGA 2 cut(s) 618, 866
MfeI CAATTG 1 cut(s) 687
MhlI GDGCHC 3 cut(s) 80, 391, 624
MluCI AATT 6 cut(s) 305, 424, 505, 687, 710, 862
MlyI GAGTC 1 cut(s) 762
MmeI TCCRAC 2 cut(s) 482, 819
MnlI CCTC 5 cut(s) 18, 79, 463, 613, 658
Mph1103I ATGCAT 1 cut(s) 666
MroI TCCGGA 1 cut(s) 311
MroXI GAANNNNTTC 1 cut(s) 598
MseI TTAA 4 cut(s) 33, 104, 377, 713
MslI CAYNNNNRTG 1 cut(s) 797
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 171
MunI CAATTG 1 cut(s) 687
Mva1269I GAATGC 2 cut(s) 124, 779
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 119, 362, 554
NdeII GATC 1 cut(s) 670
NlaIII CATG 5 cut(s) 133, 229, 268, 320, 609
NlaIV GGNNCC 2 cut(s) 6, 615
NsiI ATGCAT 1 cut(s) 666
NspI RCATGY 2 cut(s) 268, 320
PciI ACATGT 1 cut(s) 316
PctI GAATGC 2 cut(s) 124, 779
PdmI GAANNNNTTC 1 cut(s) 598
PfeI GAWTC 1 cut(s) 187
PkrI GCNGC 3 cut(s) 124, 355, 804
PleI GAGTC 1 cut(s) 761
PpsI GAGTC 1 cut(s) 761
PscI ACATGT 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 2 cut(s) 6, 615
PspPI GGNCC 1 cut(s) 4
RsaI GTAC 4 cut(s) 43, 100, 539, 564
RsaNI GTAC 4 cut(s) 42, 99, 538, 563
RseI CAYNNNNRTG 1 cut(s) 797
SaqAI TTAA 4 cut(s) 33, 104, 377, 713
SatI GCNGC 3 cut(s) 123, 354, 803
Sau3AI GATC 1 cut(s) 670
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 2 cut(s) 43, 100
SchI GAGTC 1 cut(s) 762
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 3 cut(s) 80, 391, 624
SetI ASST 9 cut(s) 71, 172, 220, 363, 377, 535, 553, 749, 839
SexAI ACCWGGT 1 cut(s) 169
SfaNI GCATC 1 cut(s) 544
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 797
SpeI ACTAGT 1 cut(s) 95
Sse9I AATT 6 cut(s) 305, 424, 505, 687, 710, 862
SsiI CCGC 1 cut(s) 692
SspMI CTAG 2 cut(s) 39, 96
StyD4I CCNGG 1 cut(s) 169
TaaI ACNGT 6 cut(s) 46, 52, 244, 286, 542, 799
TaqI TCGA 1 cut(s) 573
TasI AATT 6 cut(s) 305, 424, 505, 687, 710, 862
TatI WGTACW 3 cut(s) 41, 98, 537
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 4 cut(s) 33, 104, 377, 713
Tru9I TTAA 4 cut(s) 33, 104, 377, 713
TscAI CASTG 4 cut(s) 57, 289, 624, 817
TseI GCWGC 3 cut(s) 122, 353, 802
TspDTI ATGAA 6 cut(s) 129, 437, 498, 596, 717, 867
TspRI CASTG 4 cut(s) 57, 289, 624, 817
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 2 cut(s) 305, 424
XceI RCATGY 2 cut(s) 268, 320
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 756
XmnI GAANNNNTTC 1 cut(s) 598
XspI CTAG 2 cut(s) 39, 96
ZrmI AGTACT 2 cut(s) 43, 100
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.