Rorug05G0249300

Double-stranded RNA binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0016795
Physical Location & Seq
Reverse (-)
27342288 .. 27342839
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0249300.1

Sequence Viewer

Length: 477 bp
ATGCTTTGTAGGCGGAGCATGTGGACTCCCACTACAGATCAGATAAGAATCCTCAAGGAGATTTTCTACAATAAGGGAGTTAGGTCCCCAACTGTAGAGCAGGTTCAGAGGATCTATCTCCAGCTGAAATGGTACGGCAAGATCGAGTTCAAGAACATCTATTTTTGGTTCGTGAACCAAAGGGCTCGGGAGAAGCAGAAGAAGAGGTCCACTTCGGATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTAATGCTGGTGTGATTGCTTTTAACGGGCAGATGGGGAACTATGGTGGTTATGGATCTATGAACATGGAGATTGAAACCCTTCCTCTGTTCCCCATGCATGGTGAGGACATCTTTGGCAACATGAAGACTACTTCCGATGGAGGTGGCGGTCACGGTGGTGGCTCTTACATTTCCCTTGAGCTCAGCCTTTCCTGCGAGGGCAAAGCTGGAAAAAGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

158

Amino Acids

17.82

Weight (kDa)

9.52

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 4 - 66 1.4e-07 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016795)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27052
malus_domestica MD11G1178800.v1.1
prunus_persica Prupe.6G151600_v2.0.a1 Prupe.6G151600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0049631
rosa_laevigata RLG00000034628
rosa_multiflora Rmu_sc0013777.1_g000008
rosa_roxburghii Rroxscaffold_1G00032040
rosa_rugosa Rorug05G0249300
rosa_samantha Rh5AG325800 Rh5BG337100 Rh5CG361800 Rh5DG349300
rosa_wichuraiana Rw5G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 91
AciI CCGC 2 cut(s) 13, 402
AclWI GGATC 2 cut(s) 119, 316
AfaI GTAC 1 cut(s) 134
AfiI CCNNNNNNNGG 1 cut(s) 353
AgsI TTSAA 2 cut(s) 151, 329
AleI CACNNNNGTG 1 cut(s) 411
AluBI AGCT 3 cut(s) 124, 436, 461
AluI AGCT 3 cut(s) 124, 436, 461
Alw21I GWGCWC 1 cut(s) 438
AlwI GGATC 2 cut(s) 119, 316
Ama87I CYCGRG 1 cut(s) 186
Asp700I GAANNNNTTC 1 cut(s) 333
AspS9I GGNCC 2 cut(s) 84, 207
AsuHPI GGTGA 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 186
AvaII GGWCC 2 cut(s) 84, 207
BaeGI GKGCMC 1 cut(s) 231
BanII GRGCYC 2 cut(s) 187, 438
BbsI GAAGAC 1 cut(s) 386
Bbv12I GWGCWC 1 cut(s) 438
BccI CCATC 2 cut(s) 280, 386
BceAI ACGGC 1 cut(s) 151
BfmI CTRYAG 2 cut(s) 33, 93
BfuAI ACCTGC 1 cut(s) 91
BlpI GCTNAGC 1 cut(s) 437
Bme18I GGWCC 2 cut(s) 84, 207
BmeT110I CYCGRG 1 cut(s) 186
BmgT120I GGNCC 2 cut(s) 84, 207
BmiI GGNNCC 1 cut(s) 86
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 1 cut(s) 104
Bpu1102I GCTNAGC 1 cut(s) 437
BpuEI CTTGAG 2 cut(s) 38, 452
BsaBI GATNNNNATC 1 cut(s) 47
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse8I GATNNNNATC 1 cut(s) 47
BseGI GGATG 1 cut(s) 223
BseJI GATNNNNATC 1 cut(s) 47
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMII CTCAG 1 cut(s) 451
BseSI GKGCMC 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 186
BslFI GGGAC 1 cut(s) 70
BslI CCNNNNNNNGG 1 cut(s) 353
BsmFI GGGAC 1 cut(s) 70
BsoBI CYCGRG 1 cut(s) 186
Bsp1286I GDGCHC 3 cut(s) 187, 231, 438
Bsp143I GATC 5 cut(s) 37, 111, 141, 239, 308
Bsp1720I GCTNAGC 1 cut(s) 437
BspACI CCGC 2 cut(s) 13, 402
BspCNI CTCAG 1 cut(s) 450
BspLI GGNNCC 1 cut(s) 86
BspMI ACCTGC 1 cut(s) 91
BspPI GGATC 2 cut(s) 119, 316
BssMI GATC 5 cut(s) 37, 111, 141, 239, 308
Bst4CI ACNGT 2 cut(s) 94, 410
Bst6I CTCTTC 1 cut(s) 197
BstDEI CTNAG 1 cut(s) 437
BstF5I GGATG 1 cut(s) 223
BstKTI GATC 5 cut(s) 40, 114, 144, 242, 311
BstMBI GATC 5 cut(s) 37, 111, 141, 239, 308
BstMWI GCNNNNNNNGC 2 cut(s) 10, 447
BstNSI RCATGY 1 cut(s) 22
BstSFI CTRYAG 2 cut(s) 33, 93
BstSLI GKGCMC 1 cut(s) 231
BstV2I GAAGAC 1 cut(s) 386
BstX2I RGATCY 2 cut(s) 111, 308
BstYI RGATCY 2 cut(s) 111, 308
BtsCI GGATG 1 cut(s) 223
BveI ACCTGC 1 cut(s) 91
Cfr13I GGNCC 2 cut(s) 84, 207
Csp6I GTAC 1 cut(s) 133
CviAII CATG 7 cut(s) 19, 224, 232, 319, 349, 353, 376
CviJI RGCY 7 cut(s) 124, 185, 247, 417, 436, 441, 461
CviKI_1 RGCY 7 cut(s) 124, 185, 247, 417, 436, 441, 461
CviQI GTAC 1 cut(s) 133
DdeI CTNAG 1 cut(s) 437
DpnI GATC 5 cut(s) 39, 113, 143, 241, 310
DpnII GATC 5 cut(s) 37, 111, 141, 239, 308
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
EciI GGCGGA 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 436
Eco24I GRGCYC 2 cut(s) 187, 438
Eco47I GGWCC 2 cut(s) 84, 207
Eco53kI GAGCTC 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 186
EcoICRI GAGCTC 1 cut(s) 436
EcoO109I RGGNCCY 1 cut(s) 84
EcoT22I ATGCAT 1 cut(s) 354
EcoT38I GRGCYC 2 cut(s) 187, 438
FaeI CATG 7 cut(s) 22, 227, 235, 322, 352, 356, 379
FaqI GGGAC 1 cut(s) 70
FatI CATG 7 cut(s) 18, 223, 231, 318, 348, 352, 375
FokI GGATG 1 cut(s) 230
FriOI GRGCYC 2 cut(s) 187, 438
GsuI CTGGAG 1 cut(s) 104
Hin1II CATG 7 cut(s) 22, 227, 235, 322, 352, 356, 379
HinfI GANTC 2 cut(s) 25, 48
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 3 cut(s) 24, 175, 210
Hpy188I TCNGA 5 cut(s) 42, 108, 217, 391, 476
Hpy188III TCNNGA 3 cut(s) 151, 172, 188
Hpy8I GTNNAC 3 cut(s) 24, 175, 210
HpyAV CCTTC 1 cut(s) 344
HpyCH4III ACNGT 2 cut(s) 94, 410
HpyCH4V TGCA 2 cut(s) 235, 352
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 447
HpyF3I CTNAG 1 cut(s) 437
Hsp92II CATG 7 cut(s) 22, 227, 235, 322, 352, 356, 379
Kzo9I GATC 5 cut(s) 37, 111, 141, 239, 308
LmnI GCTCC 1 cut(s) 15
LpnPI CCDG 6 cut(s) 86, 134, 228, 246, 447, 460
MaeIII GTNAC 1 cut(s) 404
MalI GATC 5 cut(s) 39, 113, 143, 241, 310
MboI GATC 5 cut(s) 37, 111, 141, 239, 308
MboII GAAGA 3 cut(s) 211, 214, 391
MflI RGATCY 2 cut(s) 111, 308
MhlI GDGCHC 3 cut(s) 187, 231, 438
MlyI GAGTC 1 cut(s) 19
MnlI CCTC 7 cut(s) 62, 102, 198, 348, 352, 389, 445
Mph1103I ATGCAT 1 cut(s) 354
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 1 cut(s) 411
MspA1I CMGCKG 1 cut(s) 124
MwoI GCNNNNNNNGC 2 cut(s) 10, 447
NdeII GATC 5 cut(s) 37, 111, 141, 239, 308
NlaIII CATG 7 cut(s) 22, 227, 235, 322, 352, 356, 379
NlaIV GGNNCC 1 cut(s) 86
NmuCI GTSAC 1 cut(s) 404
NsiI ATGCAT 1 cut(s) 354
NspI RCATGY 1 cut(s) 22
OliI CACNNNNGTG 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 1 cut(s) 48
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 84
Psp124BI GAGCTC 1 cut(s) 438
Psp5II RGGWCCY 1 cut(s) 84
PspN4I GGNNCC 1 cut(s) 86
PspPI GGNCC 2 cut(s) 84, 207
PspPPI RGGWCCY 1 cut(s) 84
PsrI GAACNNNNNNTAC 2 cut(s) 87, 119
PsuI RGATCY 2 cut(s) 111, 308
PvuII CAGCTG 1 cut(s) 124
RsaI GTAC 1 cut(s) 134
RsaNI GTAC 1 cut(s) 133
RseI CAYNNNNRTG 1 cut(s) 411
SacI GAGCTC 1 cut(s) 438
SaqAI TTAA 1 cut(s) 276
Sau3AI GATC 5 cut(s) 37, 111, 141, 239, 308
Sau96I GGNCC 2 cut(s) 84, 207
SchI GAGTC 1 cut(s) 19
SduI GDGCHC 3 cut(s) 187, 231, 438
SetI ASST 7 cut(s) 86, 105, 126, 209, 400, 438, 463
SfcI CTRYAG 2 cut(s) 33, 93
SinI GGWCC 2 cut(s) 84, 207
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 2 cut(s) 53, 431
SmoI CTYRAG 2 cut(s) 53, 431
SsiI CCGC 2 cut(s) 13, 402
SstI GAGCTC 1 cut(s) 438
TaaI ACNGT 2 cut(s) 94, 410
TaqI TCGA 1 cut(s) 144
TfiI GAWTC 1 cut(s) 48
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TseFI GTSAC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 3 cut(s) 212, 329, 392
VpaK11BI GGWCC 2 cut(s) 84, 207
XceI RCATGY 1 cut(s) 22
XmnI GAANNNNTTC 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.