FvH4_3g29380

cysteine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014007
Physical Location & Seq
Reverse (-)
22474311 .. 22477509
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29380.t2

Sequence Viewer

Length: 1269 bp
ATGGCGCCTGTGAGAATTACAGGCACAGGCGCTGTGGTCGCTGCAATCTCAGTGACCATGGCCTTGCTCTCTGTCCTGCTCTACAAGTCCAAAAAGATCAAACCGGCCTTACCATGGAACAAAAATCCCAGAAGTGGCCTCGTTGGAGCCATTGGCAACACCCCTCTCATTCGCATCAACAGCCTCTCTGACGCCACTGGCTGTGAAATTCTTGGGAAGTGCGAGTTTTTGAACCCTGGAGGGAGTGTCAAAGATAGAGTTGCTGTCAAAATTATTGAGGAGGCTTTGAAATCTGGAGACCTTGCTCCAGGTGGAGTAGTCACTGAGGGAAGTGCTGGAAGCACAGCTATTAGCCTTGCTACAGTTGCTCCTGCTTATGGTTGCAAATGTCATGTGGTTATACCCGATGATGCTGCTATTGAGAAGTCTCAGATACTTGAAGCCCTAGGAGCCACTGTTGAGAGGGTAAGGCCAGTTTCAATCACACACAGAGATCACTTTGTCAACATTGCTAGGCAACGGGCCTTAGAAGCAAATGAGTTAGCCTCAAAGCATAGAAAAGATGAGCAAAAGAATGGCAAAGCCCTTGAGAAAGTTAATGGTTGTCATTATGATGGAGAAAAACAATTGTTTCCAAGGAATTGTCCAGGTGGATATTTTGCTGATCAATTTGAAAACTTGGCAAACTTTAGGGCACACTATGAGAGTACTGGGCCTGAGATTTGGCAGCAGACTGGTGGACATATAGATGCATTTGTGGCGGCAGCAGGAACGGGTGGTACTGTGGCTGGCACTTCCAAATTTCTACAGGAAAGGAATCCAAACATCAAATGCTTCCTGATAGACCCTCCTGGTTCTGGTTTATTTAATAAGATTACGAGGGGGGTGATGTACACCAAAGAGGAGGCTGAAGGACGAAGGCTAAAGAATCCATTTGACACGATAACTGAAGGAATTGGAATCAATAGACTAACAAAGAATTTTCTGATGGCAAAGCTTGATGGGGCTTTCCGAGGCACAGACAAGGAAGCTGTTGAAATGTCCAGGTTTCTGATGAAGAACGATGGGCTCTTCTTAGGGAGTTCTTCAGCTATGAATTGTGTTGGAGCAGTTCGAGTGGCACAGTCAATTGGTCCTGGTCACACTATTGTCACCATGTTGTGTGATAGTGGGATGAGACATCTAAGCAAATTTTACAATGCTGAGTATCTGTCTAAATTTGATTTGACACCCTCTGCTTGCGGATTAGAGTTCATGGGTGTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

423

Amino Acids

45.18

Weight (kDa)

8.8

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 19 - 316 3.5e-47 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55880 AT1G55880
fragaria_vesca FvH4_3g29380 FvH4_3g29380
malus_domestica MD03G1184200.v1.1
prunus_persica Prupe.4G242200_v2.0.a1
pyrus_communis pycom03g13750
rosa_chinensis RchiOBHm_Chr5g0053771
rosa_laevigata RLG00000034902
rosa_multiflora Rmu_sc0001759.1_g000024
rosa_roxburghii Rroxscaffold_1G00026820
rosa_rugosa Rorug05G0282500 Rorug05G0282600 Rorug05G0282700
rosa_samantha Rh5BG364700 Rh5DG379500
rosa_wichuraiana Rw5G033350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 2 cut(s) 761, 1242
AcsI RAATTY 5 cut(s) 207, 800, 979, 1190, 1217
AcuI CTGAAG 3 cut(s) 930, 969, 1071
AcyI GRCGYC 2 cut(s) 5, 192
AfaI GTAC 3 cut(s) 709, 781, 893
AfiI CCNNNNNNNGG 4 cut(s) 114, 134, 377, 857
AgsI TTSAA 6 cut(s) 232, 289, 440, 480, 674, 1037
AjnI CCWGG 6 cut(s) 235, 307, 646, 850, 1043, 1135
AluBI AGCT 4 cut(s) 347, 997, 1031, 1091
AluI AGCT 4 cut(s) 347, 997, 1031, 1091
Alw26I GTCTC 3 cut(s) 291, 432, 1171
AlwNI CAGNNNCTG 1 cut(s) 32
AoxI GGCC 6 cut(s) 60, 105, 136, 470, 522, 713
ApeKI GCWGC 4 cut(s) 41, 413, 727, 764
ApoI RAATTY 5 cut(s) 207, 800, 979, 1190, 1217
AspA2I CCTAGG 1 cut(s) 445
AspLEI GCGC 2 cut(s) 7, 32
AspS9I GGNCC 3 cut(s) 522, 713, 1133
AsuHPI GGTGA 2 cut(s) 898, 1144
AvaII GGWCC 1 cut(s) 1133
AvrII CCTAGG 1 cut(s) 445
BaeGI GKGCMC 1 cut(s) 697
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 1071
BbvI GCAGC 4 cut(s) 28, 400, 739, 776
BccI CCATC 4 cut(s) 608, 982, 995, 1058
BcgI CGANNNNNNTGC 2 cut(s) 395, 429
BciT130I CCWGG 6 cut(s) 237, 309, 648, 852, 1045, 1137
BclI TGATCA 1 cut(s) 664
BcoDI GTCTC 3 cut(s) 291, 432, 1171
BfaI CTAG 2 cut(s) 446, 513
BfmI CTRYAG 2 cut(s) 360, 806
BfoI RGCGCY 2 cut(s) 8, 33
BisI GCNGC 5 cut(s) 42, 414, 728, 762, 765
BlnI CCTAGG 1 cut(s) 445
BlsI GCNGC 5 cut(s) 43, 415, 729, 763, 766
BmcAI AGTACT 1 cut(s) 709
Bme1390I CCNGG 6 cut(s) 237, 309, 648, 852, 1045, 1137
Bme18I GGWCC 1 cut(s) 1133
BmgT120I GGNCC 3 cut(s) 522, 713, 1133
BmiI GGNNCC 3 cut(s) 6, 148, 451
BmrFI CCNGG 6 cut(s) 237, 309, 648, 852, 1045, 1137
BmrI ACTGGG 1 cut(s) 720
BmsI GCATC 3 cut(s) 183, 400, 739
BmuI ACTGGG 1 cut(s) 720
BplI GAGNNNNNCTC 2 cut(s) 530, 562
BpmI CTGGAG 3 cut(s) 258, 291, 315
BpuEI CTTGAG 1 cut(s) 608
BsaHI GRCGYC 2 cut(s) 5, 192
BsaI GGTCTC 1 cut(s) 291
BsaJI CCNNGG 6 cut(s) 57, 113, 235, 445, 635, 1012
BsaXI ACNNNNNCTCC 6 cut(s) 231, 261, 352, 382, 441, 471
Bsc4I CCNNNNNNNGG 4 cut(s) 114, 134, 377, 857
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 4 cut(s) 202, 473, 715, 739
Bse3DI GCAATG 1 cut(s) 507
BseBI CCWGG 6 cut(s) 237, 309, 648, 852, 1045, 1137
BseDI CCNNGG 6 cut(s) 57, 113, 235, 445, 635, 1012
BseGI GGATG 1 cut(s) 1179
BseLI CCNNNNNNNGG 4 cut(s) 114, 134, 377, 857
BseMI GCAATG 1 cut(s) 507
BseMII CTCAG 5 cut(s) 63, 315, 443, 708, 1194
BseNI ACTGG 4 cut(s) 202, 473, 715, 739
BseRI GAGGAG 2 cut(s) 293, 917
BseSI GKGCMC 1 cut(s) 697
BseXI GCAGC 4 cut(s) 28, 400, 739, 776
BshFI GGCC 6 cut(s) 62, 107, 138, 472, 524, 715
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 1 cut(s) 104
BslI CCNNNNNNNGG 4 cut(s) 114, 134, 377, 857
BsmAI GTCTC 3 cut(s) 291, 432, 1171
BsnI GGCC 6 cut(s) 62, 107, 138, 472, 524, 715
Bso31I GGTCTC 1 cut(s) 291
Bsp1286I GDGCHC 2 cut(s) 697, 1071
Bsp1407I TGTACA 1 cut(s) 891
Bsp143I GATC 3 cut(s) 96, 493, 664
Bsp19I CCATGG 2 cut(s) 57, 113
BspACI CCGC 2 cut(s) 761, 1242
BspANI GGCC 6 cut(s) 62, 107, 138, 472, 524, 715
BspCNI CTCAG 5 cut(s) 62, 316, 442, 709, 1195
BspLI GGNNCC 3 cut(s) 6, 148, 451
BspQI GCTCTTC 1 cut(s) 1076
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 507
BsrFI RCCGGY 1 cut(s) 103
BsrGI TGTACA 1 cut(s) 891
BsrI ACTGG 4 cut(s) 202, 473, 715, 739
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 6 cut(s) 57, 113, 235, 445, 635, 1012
BssMI GATC 3 cut(s) 96, 493, 664
BssNI GRCGYC 2 cut(s) 5, 192
BssT1I CCWWGG 4 cut(s) 57, 113, 445, 635
Bst2UI CCWGG 6 cut(s) 237, 309, 648, 852, 1045, 1137
Bst4CI ACNGT 4 cut(s) 364, 457, 784, 1125
Bst6I CTCTTC 1 cut(s) 1076
BstACI GRCGYC 2 cut(s) 5, 192
BstAUI TGTACA 1 cut(s) 891
BstC8I GCNNGC 2 cut(s) 790, 1240
BstDEI CTNAG 8 cut(s) 49, 324, 429, 526, 717, 1075, 1184, 1203
BstDSI CCRYGG 2 cut(s) 57, 113
BstF5I GGATG 1 cut(s) 1179
BstH2I RGCGCY 2 cut(s) 8, 33
BstHHI GCGC 2 cut(s) 7, 32
BstKTI GATC 3 cut(s) 99, 496, 667
BstMAI GTCTC 3 cut(s) 291, 432, 1171
BstMBI GATC 3 cut(s) 96, 493, 664
BstMWI GCNNNNNNNGC 6 cut(s) 38, 180, 365, 449, 530, 758
BstNI CCWGG 6 cut(s) 237, 309, 648, 852, 1045, 1137
BstSCI CCNGG 6 cut(s) 235, 307, 646, 850, 1043, 1135
BstSFI CTRYAG 2 cut(s) 360, 806
BstSLI GKGCMC 1 cut(s) 697
BstV1I GCAGC 4 cut(s) 28, 400, 739, 776
BsuRI GGCC 6 cut(s) 62, 107, 138, 472, 524, 715
BtgI CCRYGG 2 cut(s) 57, 113
BtsCI GGATG 1 cut(s) 1179
BtsIMutI CAGTG 4 cut(s) 57, 195, 321, 453
Cac8I GCNNGC 2 cut(s) 790, 1240
CaiI CAGNNNCTG 1 cut(s) 32
CfoI GCGC 2 cut(s) 7, 32
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 3 cut(s) 522, 713, 1133
CseI GACGC 1 cut(s) 200
Csp6I GTAC 3 cut(s) 708, 780, 892
CviAII CATG 5 cut(s) 58, 114, 392, 1156, 1255
CviQI GTAC 3 cut(s) 708, 780, 892
DdeI CTNAG 8 cut(s) 49, 324, 429, 526, 717, 1075, 1184, 1203
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 98, 495, 666
DpnII GATC 3 cut(s) 96, 493, 664
Eam1104I CTCTTC 1 cut(s) 1076
EarI CTCTTC 1 cut(s) 1076
Eco130I CCWWGG 4 cut(s) 57, 113, 445, 635
Eco24I GRGCYC 1 cut(s) 1071
Eco31I GGTCTC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 1133
Eco57I CTGAAG 3 cut(s) 930, 969, 1071
EcoRII CCWGG 6 cut(s) 235, 307, 646, 850, 1043, 1135
EcoT14I CCWWGG 4 cut(s) 57, 113, 445, 635
EcoT22I ATGCAT 1 cut(s) 754
EcoT38I GRGCYC 1 cut(s) 1071
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 57, 113, 445, 635
FaeI CATG 5 cut(s) 61, 117, 395, 1159, 1258
FatI CATG 5 cut(s) 57, 113, 391, 1155, 1254
FbaI TGATCA 1 cut(s) 664
Fnu4HI GCNGC 5 cut(s) 42, 414, 728, 762, 765
FokI GGATG 1 cut(s) 1186
FriOI GRGCYC 1 cut(s) 1071
Fsp4HI GCNGC 5 cut(s) 42, 414, 728, 762, 765
FspBI CTAG 2 cut(s) 446, 513
GlaI GCGC 2 cut(s) 6, 31
GluI GCNGC 5 cut(s) 42, 414, 728, 762, 765
GsuI CTGGAG 3 cut(s) 258, 291, 315
HaeII RGCGCY 2 cut(s) 8, 33
HaeIII GGCC 6 cut(s) 62, 107, 138, 472, 524, 715
HapII CCGG 1 cut(s) 104
HgaI GACGC 1 cut(s) 200
HhaI GCGC 2 cut(s) 7, 32
Hin1I GRCGYC 2 cut(s) 5, 192
Hin1II CATG 5 cut(s) 61, 117, 395, 1159, 1258
Hin6I GCGC 2 cut(s) 5, 30
HinP1I GCGC 2 cut(s) 5, 30
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HindIII AAGCTT 1 cut(s) 995
HinfI GANTC 3 cut(s) 817, 928, 960
HpaII CCGG 1 cut(s) 104
HphI GGTGA 2 cut(s) 898, 1144
Hpy166II GTNNAC 3 cut(s) 505, 740, 894
Hpy188I TCNGA 5 cut(s) 190, 432, 987, 1013, 1053
Hpy188III TCNNGA 2 cut(s) 294, 838
Hpy8I GTNNAC 3 cut(s) 505, 740, 894
HpyAV CCTTC 3 cut(s) 905, 912, 944
HpyCH4III ACNGT 4 cut(s) 364, 457, 784, 1125
HpyCH4V TGCA 3 cut(s) 44, 384, 752
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 180, 365, 449, 530, 758
HpyF3I CTNAG 8 cut(s) 49, 324, 429, 526, 717, 1075, 1184, 1203
Hsp92I GRCGYC 2 cut(s) 5, 192
Hsp92II CATG 5 cut(s) 61, 117, 395, 1159, 1258
HspAI GCGC 2 cut(s) 5, 30
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 664
Kzo9I GATC 3 cut(s) 96, 493, 664
LguI GCTCTTC 1 cut(s) 1076
LmnI GCTCC 5 cut(s) 146, 310, 373, 449, 1106
Lsp1109I GCAGC 4 cut(s) 28, 400, 739, 776
LweI GCATC 3 cut(s) 183, 400, 739
MaeI CTAG 2 cut(s) 446, 513
MaeIII GTNAC 4 cut(s) 52, 319, 1139, 1150
MalI GATC 3 cut(s) 98, 495, 666
MboI GATC 3 cut(s) 96, 493, 664
MboII GAAGA 3 cut(s) 1063, 1069, 1077
MfeI CAATTG 2 cut(s) 626, 1128
MhlI GDGCHC 2 cut(s) 697, 1071
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 124, 1084, 1243
Mph1103I ATGCAT 1 cut(s) 754
MseI TTAA 2 cut(s) 597, 867
MslI CAYNNNNRTG 2 cut(s) 612, 747
MspI CCGG 1 cut(s) 104
MspR9I CCNGG 6 cut(s) 237, 309, 648, 852, 1045, 1137
MunI CAATTG 2 cut(s) 626, 1128
MvaI CCWGG 6 cut(s) 237, 309, 648, 852, 1045, 1137
MwoI GCNNNNNNNGC 6 cut(s) 38, 180, 365, 449, 530, 758
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 2 cut(s) 57, 113
NdeII GATC 3 cut(s) 96, 493, 664
NlaIII CATG 5 cut(s) 61, 117, 395, 1159, 1258
NlaIV GGNNCC 3 cut(s) 6, 148, 451
NmuCI GTSAC 4 cut(s) 52, 319, 1139, 1150
NsiI ATGCAT 1 cut(s) 754
PciSI GCTCTTC 1 cut(s) 1076
PfeI GAWTC 3 cut(s) 817, 928, 960
PkrI GCNGC 5 cut(s) 43, 415, 729, 763, 766
PluTI GGCGCC 1 cut(s) 8
Psp6I CCWGG 6 cut(s) 235, 307, 646, 850, 1043, 1135
PspGI CCWGG 6 cut(s) 235, 307, 646, 850, 1043, 1135
PspN4I GGNNCC 3 cut(s) 6, 148, 451
PspPI GGNCC 3 cut(s) 522, 713, 1133
PsrI GAACNNNNNNTAC 2 cut(s) 763, 795
PstNI CAGNNNCTG 1 cut(s) 32
RsaI GTAC 3 cut(s) 709, 781, 893
RsaNI GTAC 3 cut(s) 708, 780, 892
RseI CAYNNNNRTG 2 cut(s) 612, 747
SapI GCTCTTC 1 cut(s) 1076
SaqAI TTAA 2 cut(s) 597, 867
SatI GCNGC 5 cut(s) 42, 414, 728, 762, 765
Sau3AI GATC 3 cut(s) 96, 493, 664
Sau96I GGNCC 3 cut(s) 522, 713, 1133
ScaI AGTACT 1 cut(s) 709
ScrFI CCNGG 6 cut(s) 237, 309, 648, 852, 1045, 1137
SduI GDGCHC 2 cut(s) 697, 1071
SetI ASST 8 cut(s) 303, 313, 349, 652, 999, 1033, 1049, 1093
SfaNI GCATC 3 cut(s) 183, 400, 739
SfcI CTRYAG 2 cut(s) 360, 806
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 1133
SmiMI CAYNNNNRTG 2 cut(s) 612, 747
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
SsiI CCGC 2 cut(s) 761, 1242
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 2 cut(s) 446, 513
StyD4I CCNGG 6 cut(s) 235, 307, 646, 850, 1043, 1135
StyI CCWWGG 4 cut(s) 57, 113, 445, 635
TaaI ACNGT 4 cut(s) 364, 457, 784, 1125
TaqI TCGA 1 cut(s) 1114
TatI WGTACW 2 cut(s) 707, 891
TauI GCSGC 1 cut(s) 764
TfiI GAWTC 3 cut(s) 817, 928, 960
Tru1I TTAA 2 cut(s) 597, 867
Tru9I TTAA 2 cut(s) 597, 867
TscAI CASTG 4 cut(s) 57, 202, 328, 460
TseFI GTSAC 4 cut(s) 52, 319, 1139, 1150
TseI GCWGC 4 cut(s) 41, 413, 727, 764
Tsp45I GTSAC 4 cut(s) 52, 319, 1139, 1150
TspDTI ATGAA 3 cut(s) 1070, 1109, 1243
TspRI CASTG 4 cut(s) 57, 202, 328, 460
VpaK11BI GGWCC 1 cut(s) 1133
XapI RAATTY 5 cut(s) 207, 800, 979, 1190, 1217
XmaJI CCTAGG 1 cut(s) 445
XspI CTAG 2 cut(s) 446, 513
ZrmI AGTACT 1 cut(s) 709
Zsp2I ATGCAT 1 cut(s) 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.