Prupe.4G242200_v2.0.a1

cysteine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0014007
Physical Location & Seq
Forward (+)
15993748 .. 15996931
3184 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G242200.1

Sequence Viewer

Length: 1278 bp
ATGGCGCCTGTGAGAACCACAGACGCTGTTGTCGCTGCAATCTCAGTGACCATTGCGTTGCTCTCTCTCTTCCTCTACAAGTCCAAGCCCTCCTCTAAAAAGCTACCCTCAAAGAAAAATCCTAGAAATGGCCTCCTTGGTGCCATTGGAAACACCCCTCTCATTCGCATCAACAGCCTCTCCGAGGCGACCGGTTGTGAAATACTTGGCAAGTGCGAATTTTTGAACCCAGGAGGGAGTGTGAAAGACAGAGTTGCTGTGAAAATCATTGAAGAGGCTTTGGAATCTGGAGACCTTGCTCCAGGTGGAGTAGTCACTGAGGGGAGTGTTGGAAGCACAGCTATTAGCCTTGCTACTGTTGCTCCAGCTTATGGTTGCAAATGCCACGTGGTTATACCTGATGACGTTGCTATTGAGAAGTCTCAAATACTTGAAGCCCTGGGAGCCACTGTAGAAAGGGTAAGGCCAGTGTCAATCACACACAGAGACCACTTCGTCAATGTTGCCAAACGACGGGCATCAGAAGCAAATGAGTTTGCATTAAAGCATAGAAAAGATGATGCGAATCAGAGGGGTGTTGCCAATGGCACCACCGAGAAGACTAATGGTTTCCAATATGATGAAGAGAAACAAAGTTTGTTTTCAAGGAACTGTACAGGTGGTTACTTTGCTGATCAGTTTGAAAATCTGGCAAACTTCCGGGCCCACTATGAGGCTACTGGGCCTGAGATTTGGGAACAGACTGGAGGAAATGTAAATGCATTTGTGGCGGGAGCAGGAACAGGTGGTACTCTGGCAGGTGTTTCTAAGTTTCTCCAGGAAAGGAATTCAAACATTAAATGCTTCCTAATAGATCCTCCTGGTTCCGGTTTATTCAATAAAGTTACGAGGGGAGTGATGTACACCAGAGAGGAGGCTGAAGGACAAAGGCTAAAGAATCCATTTGACACGATAACTGAAGGAATTGGAATCAATAGAATAACAAAGAATTTTATGATGGCAAAACTTGATGGGGCTTTCCGAGGCACAGATAAGGAAGCTGTTGAAATGTCCAGGTTTCTTATGAAGAATGACGGGCTGTTTTTGGGGAGTTCTTCAGCCATGAATTGTGTTGGAGCAGTACGAGCGGCACAGTCAATTGGTCCTGGTCACACAATTGTGACTATTTTGTGCGATAGTGGGATGAGACATCTGAGCAAATTTTTCAGTGCTGATTATCTATCACAATATGGTTTGACACCTTCGGCTAGCGGATTAGAGTTCCTGGGTGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

426

Amino Acids

45.4

Weight (kDa)

8.32

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55880 AT1G55880
fragaria_vesca FvH4_3g29380 FvH4_3g29380
malus_domestica MD03G1184200.v1.1
prunus_persica Prupe.4G242200_v2.0.a1
pyrus_communis pycom03g13750
rosa_chinensis RchiOBHm_Chr5g0053771
rosa_laevigata RLG00000034902
rosa_multiflora Rmu_sc0001759.1_g000024
rosa_roxburghii Rroxscaffold_1G00026820
rosa_rugosa Rorug05G0282500 Rorug05G0282600 Rorug05G0282700
rosa_samantha Rh5BG364700 Rh5DG379500
rosa_wichuraiana Rw5G033350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 788
AasI GACNNNNNNGTC 2 cut(s) 29, 494
Acc36I ACCTGC 1 cut(s) 788
AccB1I GGYRCC 3 cut(s) 4, 140, 587
AccB7I CCANNNNNTGG 1 cut(s) 371
AccBSI CCGCTC 1 cut(s) 1127
AciI CCGC 3 cut(s) 770, 1127, 1251
AclWI GGATC 1 cut(s) 848
AcsI RAATTY 4 cut(s) 218, 826, 988, 1199
AcuI CTGAAG 3 cut(s) 939, 978, 1080
AcvI CACGTG 1 cut(s) 388
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 655, 790, 902, 1122
AfiI CCNNNNNNNGG 6 cut(s) 128, 184, 371, 513, 712, 866
AgeI ACCGGT 1 cut(s) 191
AgsI TTSAA 8 cut(s) 226, 272, 434, 645, 683, 831, 877, 1046
AjnI CCWGG 8 cut(s) 229, 301, 438, 816, 859, 1052, 1144, 1263
AleI CACNNNNGTG 1 cut(s) 1157
AluBI AGCT 4 cut(s) 103, 341, 368, 1040
AluI AGCT 4 cut(s) 103, 341, 368, 1040
Alw26I GTCTC 4 cut(s) 285, 426, 480, 1180
AlwI GGATC 1 cut(s) 848
AlwNI CAGNNNCTG 1 cut(s) 26
AoxI GGCC 4 cut(s) 130, 464, 702, 722
ApaI GGGCCC 1 cut(s) 706
ApeKI GCWGC 1 cut(s) 35
ApoI RAATTY 4 cut(s) 218, 826, 988, 1199
AsiGI ACCGGT 1 cut(s) 191
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 4 cut(s) 702, 703, 722, 1142
AsuC2I CCSGG 1 cut(s) 701
AsuNHI GCTAGC 1 cut(s) 1247
AvaII GGWCC 1 cut(s) 1142
BaeGI GKGCMC 1 cut(s) 706
BanI GGYRCC 3 cut(s) 4, 140, 587
BanII GRGCYC 1 cut(s) 706
BbrPI CACGTG 1 cut(s) 388
BbsI GAAGAC 1 cut(s) 605
BbvI GCAGC 1 cut(s) 22
BccI CCATC 2 cut(s) 991, 1004
BciT130I CCWGG 8 cut(s) 231, 303, 440, 818, 861, 1054, 1146, 1265
BclI TGATCA 1 cut(s) 673
BcnI CCSGG 1 cut(s) 701
BcoDI GTCTC 4 cut(s) 285, 426, 480, 1180
BfaI CTAG 2 cut(s) 123, 1248
BfmI CTRYAG 1 cut(s) 450
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 788
BisI GCNGC 2 cut(s) 36, 1128
BlsI GCNGC 2 cut(s) 37, 1129
Bme1390I CCNGG 9 cut(s) 231, 303, 440, 701, 818, 861, 1054, 1146, 1265
Bme18I GGWCC 1 cut(s) 1142
BmgT120I GGNCC 4 cut(s) 702, 703, 722, 1142
BmiI GGNNCC 6 cut(s) 6, 142, 445, 589, 704, 865
BmrFI CCNGG 9 cut(s) 231, 303, 440, 701, 818, 861, 1054, 1146, 1265
BmrI ACTGGG 1 cut(s) 729
BmsI GCATC 3 cut(s) 177, 527, 550
BmtI GCTAGC 1 cut(s) 1251
BmuI ACTGGG 1 cut(s) 729
BpiI GAAGAC 1 cut(s) 605
BpmI CTGGAG 5 cut(s) 285, 309, 348, 765, 800
BpuMI CCSGG 1 cut(s) 701
BsaAI YACGTR 1 cut(s) 388
BsaBI GATNNNNATC 1 cut(s) 564
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 285, 480
BsaJI CCNNGG 7 cut(s) 136, 183, 229, 438, 439, 1021, 1264
BsaWI WCCGGW 2 cut(s) 191, 866
Bsc4I CCNNNNNNNGG 6 cut(s) 128, 184, 371, 513, 712, 866
Bse118I RCCGGY 1 cut(s) 191
Bse1I ACTGG 3 cut(s) 467, 724, 748
Bse3DI GCAATG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 8 cut(s) 231, 303, 440, 818, 861, 1054, 1146, 1265
BseDI CCNNGG 7 cut(s) 136, 183, 229, 438, 439, 1021, 1264
BseGI GGATG 1 cut(s) 1188
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 6 cut(s) 128, 184, 371, 513, 712, 866
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 4 cut(s) 57, 309, 717, 1184
BseNI ACTGG 3 cut(s) 467, 724, 748
BseRI GAGGAG 2 cut(s) 82, 926
BseSI GKGCMC 1 cut(s) 706
BseXI GCAGC 1 cut(s) 22
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 4 cut(s) 132, 466, 704, 724
BshNI GGYRCC 3 cut(s) 4, 140, 587
BshTI ACCGGT 1 cut(s) 191
BsiEI CGRYCG 1 cut(s) 192
BsiSI CCGG 3 cut(s) 192, 700, 867
BslI CCNNNNNNNGG 6 cut(s) 128, 184, 371, 513, 712, 866
BsmAI GTCTC 4 cut(s) 285, 426, 480, 1180
BsnI GGCC 4 cut(s) 132, 466, 704, 724
Bso31I GGTCTC 2 cut(s) 285, 480
Bsp120I GGGCCC 1 cut(s) 702
Bsp1286I GDGCHC 1 cut(s) 706
Bsp1407I TGTACA 2 cut(s) 653, 900
Bsp143I GATC 2 cut(s) 673, 853
BspACI CCGC 3 cut(s) 770, 1127, 1251
BspANI GGCC 4 cut(s) 132, 466, 704, 724
BspCNI CTCAG 4 cut(s) 56, 310, 718, 1185
BspLI GGNNCC 6 cut(s) 6, 142, 445, 589, 704, 865
BspMI ACCTGC 1 cut(s) 788
BspOI GCTAGC 1 cut(s) 1251
BspPI GGATC 1 cut(s) 848
BspT107I GGYRCC 3 cut(s) 4, 140, 587
BspTNI GGTCTC 2 cut(s) 285, 480
BsrBI CCGCTC 1 cut(s) 1127
BsrDI GCAATG 1 cut(s) 51
BsrFI RCCGGY 1 cut(s) 191
BsrGI TGTACA 2 cut(s) 653, 900
BsrI ACTGG 3 cut(s) 467, 724, 748
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 7 cut(s) 136, 183, 229, 438, 439, 1021, 1264
BssMI GATC 2 cut(s) 673, 853
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 8 cut(s) 231, 303, 440, 818, 861, 1054, 1146, 1265
Bst4CI ACNGT 4 cut(s) 358, 451, 653, 1134
Bst6I CTCTTC 3 cut(s) 74, 267, 618
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 2 cut(s) 653, 900
BstBAI YACGTR 1 cut(s) 388
BstC8I GCNNGC 1 cut(s) 1249
BstDEI CTNAG 5 cut(s) 43, 318, 726, 807, 1193
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 1 cut(s) 1188
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 676, 856
BstMAI GTCTC 4 cut(s) 285, 426, 480, 1180
BstMBI GATC 2 cut(s) 673, 853
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 7 cut(s) 32, 174, 359, 443, 524, 767, 1124
BstNI CCWGG 8 cut(s) 231, 303, 440, 818, 861, 1054, 1146, 1265
BstSCI CCNGG 9 cut(s) 229, 301, 438, 699, 816, 859, 1052, 1144, 1263
BstSFI CTRYAG 1 cut(s) 450
BstSLI GKGCMC 1 cut(s) 706
BstV1I GCAGC 1 cut(s) 22
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
BsuRI GGCC 4 cut(s) 132, 466, 704, 724
BtsCI GGATG 1 cut(s) 1188
BtsIMutI CAGTG 5 cut(s) 51, 315, 447, 474, 1213
BveI ACCTGC 1 cut(s) 788
Cac8I GCNNGC 1 cut(s) 1249
CaiI CAGNNNCTG 1 cut(s) 26
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 4 cut(s) 702, 703, 722, 1142
CseI GACGC 1 cut(s) 32
Csp6I GTAC 4 cut(s) 654, 789, 901, 1121
CspAI ACCGGT 1 cut(s) 191
CviAII CATG 1 cut(s) 1102
CviQI GTAC 4 cut(s) 654, 789, 901, 1121
DdeI CTNAG 5 cut(s) 43, 318, 726, 807, 1193
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 675, 855
DpnII GATC 2 cut(s) 673, 853
DrdI GACNNNNNNGTC 2 cut(s) 29, 494
DseDI GACNNNNNNGTC 2 cut(s) 29, 494
Eam1104I CTCTTC 3 cut(s) 74, 267, 618
EarI CTCTTC 3 cut(s) 74, 267, 618
Eco130I CCWWGG 1 cut(s) 136
Eco24I GRGCYC 1 cut(s) 706
Eco31I GGTCTC 2 cut(s) 285, 480
Eco47I GGWCC 1 cut(s) 1142
Eco57I CTGAAG 3 cut(s) 939, 978, 1080
Eco72I CACGTG 1 cut(s) 388
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 826
EcoRII CCWGG 8 cut(s) 229, 301, 438, 816, 859, 1052, 1144, 1263
EcoT14I CCWWGG 1 cut(s) 136
EcoT22I ATGCAT 1 cut(s) 763
EcoT38I GRGCYC 1 cut(s) 706
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 1 cut(s) 1105
FaiI YATR 9 cut(s) 372, 395, 549, 618, 711, 995, 1064, 1103, 1230
FatI CATG 1 cut(s) 1101
FauI CCCGC 1 cut(s) 763
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 2 cut(s) 36, 1128
FokI GGATG 1 cut(s) 1195
FriOI GRGCYC 1 cut(s) 706
Fsp4HI GCNGC 2 cut(s) 36, 1128
FspBI CTAG 2 cut(s) 123, 1248
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 36, 1128
GsuI CTGGAG 5 cut(s) 285, 309, 348, 765, 800
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 132, 466, 704, 724
HapII CCGG 3 cut(s) 192, 700, 867
HgaI GACGC 1 cut(s) 32
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 1105
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 4 cut(s) 284, 565, 937, 969
HpaII CCGG 3 cut(s) 192, 700, 867
Hpy166II GTNNAC 1 cut(s) 903
Hpy188I TCNGA 5 cut(s) 184, 523, 570, 1022, 1194
Hpy188III TCNNGA 1 cut(s) 288
Hpy8I GTNNAC 1 cut(s) 903
Hpy99I CGWCG 1 cut(s) 516
HpyAV CCTTC 3 cut(s) 914, 953, 1251
HpyCH4III ACNGT 4 cut(s) 358, 451, 653, 1134
HpyCH4IV ACGT 2 cut(s) 387, 405
HpyCH4V TGCA 4 cut(s) 38, 378, 539, 761
HpyF10VI GCNNNNNNNGC 7 cut(s) 32, 174, 359, 443, 524, 767, 1124
HpyF3I CTNAG 5 cut(s) 43, 318, 726, 807, 1193
HpySE526I ACGT 2 cut(s) 387, 405
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 1105
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 2 cut(s) 673, 853
LmnI GCTCC 5 cut(s) 304, 367, 443, 773, 1115
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 3 cut(s) 177, 527, 550
MaeI CTAG 2 cut(s) 123, 1248
MaeII ACGT 2 cut(s) 387, 405
MaeIII GTNAC 6 cut(s) 46, 313, 662, 883, 1148, 1159
MalI GATC 2 cut(s) 675, 855
MbiI CCGCTC 1 cut(s) 1127
MboI GATC 2 cut(s) 673, 853
MboII GAAGA 6 cut(s) 61, 284, 610, 635, 1078, 1086
MfeI CAATTG 2 cut(s) 1137, 1155
MflI RGATCY 1 cut(s) 853
MhlI GDGCHC 1 cut(s) 706
MluCI AATT 8 cut(s) 218, 826, 963, 988, 1105, 1137, 1155, 1199
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 310, 1093, 1252
Mph1103I ATGCAT 1 cut(s) 763
MseI TTAA 2 cut(s) 542, 837
MslI CAYNNNNRTG 1 cut(s) 1157
MspI CCGG 3 cut(s) 192, 700, 867
MspR9I CCNGG 9 cut(s) 231, 303, 440, 701, 818, 861, 1054, 1146, 1265
MunI CAATTG 2 cut(s) 1137, 1155
MvaI CCWGG 8 cut(s) 231, 303, 440, 818, 861, 1054, 1146, 1265
MwoI GCNNNNNNNGC 7 cut(s) 32, 174, 359, 443, 524, 767, 1124
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 701
NdeII GATC 2 cut(s) 673, 853
NheI GCTAGC 1 cut(s) 1247
NlaIII CATG 1 cut(s) 1105
NlaIV GGNNCC 6 cut(s) 6, 142, 445, 589, 704, 865
NmuCI GTSAC 4 cut(s) 46, 313, 1148, 1159
NsiI ATGCAT 1 cut(s) 763
OliI CACNNNNGTG 1 cut(s) 1157
PaqCI CACCTGC 1 cut(s) 788
PasI CCCWGGG 1 cut(s) 439
PfeI GAWTC 4 cut(s) 284, 565, 937, 969
PflMI CCANNNNNTGG 1 cut(s) 371
PfoI TCCNGGA 1 cut(s) 816
PinAI ACCGGT 1 cut(s) 191
PkrI GCNGC 2 cut(s) 37, 1129
PluTI GGCGCC 1 cut(s) 8
PmaCI CACGTG 1 cut(s) 388
PmlI CACGTG 1 cut(s) 388
Ppu21I YACGTR 1 cut(s) 388
Psp6I CCWGG 8 cut(s) 229, 301, 438, 816, 859, 1052, 1144, 1263
PspCI CACGTG 1 cut(s) 388
PspGI CCWGG 8 cut(s) 229, 301, 438, 816, 859, 1052, 1144, 1263
PspN4I GGNNCC 6 cut(s) 6, 142, 445, 589, 704, 865
PspOMI GGGCCC 1 cut(s) 702
PspPI GGNCC 4 cut(s) 702, 703, 722, 1142
PsrI GAACNNNNNNTAC 2 cut(s) 772, 804
PstNI CAGNNNCTG 1 cut(s) 26
PsuI RGATCY 1 cut(s) 853
RsaI GTAC 4 cut(s) 655, 790, 902, 1122
RsaNI GTAC 4 cut(s) 654, 789, 901, 1121
RseI CAYNNNNRTG 1 cut(s) 1157
SaqAI TTAA 2 cut(s) 542, 837
SatI GCNGC 2 cut(s) 36, 1128
Sau3AI GATC 2 cut(s) 673, 853
Sau96I GGNCC 4 cut(s) 702, 703, 722, 1142
ScrFI CCNGG 9 cut(s) 231, 303, 440, 701, 818, 861, 1054, 1146, 1265
SduI GDGCHC 1 cut(s) 706
SfaNI GCATC 3 cut(s) 177, 527, 550
SfcI CTRYAG 1 cut(s) 450
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 1142
SmiMI CAYNNNNRTG 1 cut(s) 1157
Sse9I AATT 8 cut(s) 218, 826, 963, 988, 1105, 1137, 1155, 1199
SsiI CCGC 3 cut(s) 770, 1127, 1251
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 2 cut(s) 123, 1248
StyD4I CCNGG 9 cut(s) 229, 301, 438, 699, 816, 859, 1052, 1144, 1263
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 4 cut(s) 358, 451, 653, 1134
TaiI ACGT 2 cut(s) 390, 408
TasI AATT 8 cut(s) 218, 826, 963, 988, 1105, 1137, 1155, 1199
TatI WGTACW 2 cut(s) 653, 900
TauI GCSGC 1 cut(s) 1130
TfiI GAWTC 4 cut(s) 284, 565, 937, 969
Tru1I TTAA 2 cut(s) 542, 837
Tru9I TTAA 2 cut(s) 542, 837
TscAI CASTG 5 cut(s) 51, 322, 454, 474, 1213
TseFI GTSAC 4 cut(s) 46, 313, 1148, 1159
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 4 cut(s) 46, 313, 1148, 1159
TspDTI ATGAA 3 cut(s) 636, 1079, 1118
TspRI CASTG 5 cut(s) 51, 322, 454, 474, 1213
Van91I CCANNNNNTGG 1 cut(s) 371
VpaK11BI GGWCC 1 cut(s) 1142
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 4 cut(s) 218, 826, 988, 1199
XspI CTAG 2 cut(s) 123, 1248
Zsp2I ATGCAT 1 cut(s) 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.