pycom03g13750

cysteine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0014007
Physical Location & Seq
Forward (+)
14857423 .. 14859397
1975 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13750.3

Sequence Viewer

Length: 960 bp
ATGGCGCCTGTGAGAAGCACAGGCGCTATTGTGGCTGCAGTCTCAGTGTCAATGGCGTTGCTCTCTCTCTTCCTCTACAAGTCCAAACTCTCCTCCAAAAAGACCAAACTAACCCTGGCCTCGAAGAAAAATCACACGAATGGCCTCGTTGGTGCCATCGGCAACACCCCTCTGATCCGCATCAACAGCCTCTCTGACGCCACCGGCTGTGAGATTCTTGGAAAGTGCGAGTTTTTGAACCCGGGAGGGAGTGTGAAAGACAGAGTTGCTGTCAAAATTATTGAAGAGGCTTTGGAATCTGGAGACCTTGCTCCAGGTGGAGTAGTCACTGAGGGGAGTGCAGGAAGCACAGCTATAAGCCTTGCTACGGTCGCTCCTGCTTATGGTTGCAAATGCCACGTGGTTATACCTGATGACGCTGCTGTTGAGAAGTCTCAAATACTTGAAGCTCTGGGAGCGACTGTTGAAAGGGTAAGACCAGTGTCAATTACGCACAGAGACCACTTTGTGAATATTGCTAGGCGACGGGCAACGGAAGCAAATGAATTAGCAATGAAGCATAGGAAAGATGAACAAATGAATGGCCAAGCCCTTGGGCAGACTAATGGTTTCCAATGTGATGGAGAGAAACAAAGTGTGGTCTCAAGTTACAAAGGTGGTTACTTTGCTGATCAGTTTGAAAATATGGCAAACTTCCGGGCCCACTATGAGGGTACCGGGCCTGAAATTTGGGAACAGACCGGAGGAAATGTGGATGCATTTGTGGCGGCAGCAGGAACAGGTGGTACCCTGGCTGGTGTGTCCAGGTTTCTCCAGGAGAAGAAGTCTAATGTCAAGTGCTTCCTGATAGATCCCCCTGGTTCCGGTTTATACAATAAAGTTACAAGGGGAGTGATGTACACTAGAGAGGAGGCTGAAGGACGAAGGCTAAAAAATCCATTTGACACGATAACTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

320

Amino Acids

33.96

Weight (kDa)

8.44

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55880 AT1G55880
fragaria_vesca FvH4_3g29380 FvH4_3g29380
malus_domestica MD03G1184200.v1.1
prunus_persica Prupe.4G242200_v2.0.a1
pyrus_communis pycom03g13750
rosa_chinensis RchiOBHm_Chr5g0053771
rosa_laevigata RLG00000034902
rosa_multiflora Rmu_sc0001759.1_g000024
rosa_roxburghii Rroxscaffold_1G00026820
rosa_rugosa Rorug05G0282500 Rorug05G0282600 Rorug05G0282700
rosa_samantha Rh5BG364700 Rh5DG379500
rosa_wichuraiana Rw5G033350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 713, 785
AccB1I GGYRCC 4 cut(s) 4, 152, 713, 785
AciI CCGC 2 cut(s) 178, 767
AclWI GGATC 2 cut(s) 169, 845
AcoI YGGCCR 1 cut(s) 583
AcsI RAATTY 1 cut(s) 726
AcuI CTGAAG 1 cut(s) 936
AcvI CACGTG 1 cut(s) 400
AcyI GRCGYC 2 cut(s) 5, 198
AdeI CACNNNGTG 1 cut(s) 508
AfaI GTAC 3 cut(s) 715, 787, 899
AfiI CCNNNNNNNGG 4 cut(s) 367, 383, 709, 863
AgsI TTSAA 5 cut(s) 238, 284, 446, 467, 680
AjnI CCWGG 6 cut(s) 114, 313, 789, 803, 813, 856
AluBI AGCT 2 cut(s) 353, 449
AluI AGCT 2 cut(s) 353, 449
Alw26I GTCTC 5 cut(s) 46, 297, 438, 492, 646
AlwI GGATC 2 cut(s) 169, 845
Ama87I CYCGRG 1 cut(s) 241
AoxI GGCC 5 cut(s) 117, 142, 583, 699, 719
ApaI GGGCCC 1 cut(s) 703
ApeKI GCWGC 3 cut(s) 35, 419, 770
ApoI RAATTY 1 cut(s) 726
Asp718I GGTACC 2 cut(s) 713, 785
AspLEI GCGC 2 cut(s) 7, 26
AspS9I GGNCC 3 cut(s) 699, 700, 719
AsuC2I CCSGG 4 cut(s) 242, 243, 698, 718
AvaI CYCGRG 1 cut(s) 241
BaeGI GKGCMC 1 cut(s) 703
BalI TGGCCA 1 cut(s) 585
BanI GGYRCC 4 cut(s) 4, 152, 713, 785
BanII GRGCYC 1 cut(s) 703
BbrPI CACGTG 1 cut(s) 400
BbvI GCAGC 3 cut(s) 22, 406, 782
BccI CCATC 2 cut(s) 164, 614
BciT130I CCWGG 6 cut(s) 116, 315, 791, 805, 815, 858
BclI TGATCA 1 cut(s) 670
BcnI CCSGG 4 cut(s) 242, 243, 698, 718
BcoDI GTCTC 5 cut(s) 46, 297, 438, 492, 646
BfaI CTAG 2 cut(s) 519, 903
BfmI CTRYAG 1 cut(s) 36
BfoI RGCGCY 2 cut(s) 8, 27
BisI GCNGC 4 cut(s) 36, 420, 768, 771
BlsI GCNGC 4 cut(s) 37, 421, 769, 772
BmeT110I CYCGRG 1 cut(s) 241
BmgT120I GGNCC 3 cut(s) 699, 700, 719
BmiI GGNNCC 6 cut(s) 6, 154, 701, 715, 787, 862
BmsI GCATC 2 cut(s) 189, 745
BoxI GACNNNNGTC 1 cut(s) 481
BpmI CTGGAG 3 cut(s) 297, 321, 797
BpuEI CTTGAG 1 cut(s) 628
BpuMI CCSGG 4 cut(s) 242, 243, 698, 718
BsaAI YACGTR 1 cut(s) 400
BsaBI GATNNNNATC 1 cut(s) 179
BsaHI GRCGYC 2 cut(s) 5, 198
BsaI GGTCTC 3 cut(s) 297, 492, 646
BsaJI CCNNGG 5 cut(s) 114, 241, 592, 789, 856
BsaWI WCCGGW 2 cut(s) 740, 863
Bsc4I CCNNNNNNNGG 4 cut(s) 367, 383, 709, 863
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 479
Bse3DI GCAATG 1 cut(s) 558
Bse8I GATNNNNATC 1 cut(s) 179
BseBI CCWGG 6 cut(s) 116, 315, 791, 805, 815, 858
BseDI CCNNGG 5 cut(s) 114, 241, 592, 789, 856
BseGI GGATG 1 cut(s) 760
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 4 cut(s) 367, 383, 709, 863
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 2 cut(s) 57, 321
BseNI ACTGG 1 cut(s) 479
BseRI GAGGAG 2 cut(s) 82, 923
BseSI GKGCMC 1 cut(s) 703
BseXI GCAGC 3 cut(s) 22, 406, 782
BsgI GTGCAG 1 cut(s) 360
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 5 cut(s) 119, 144, 585, 701, 721
BshNI GGYRCC 4 cut(s) 4, 152, 713, 785
BsiEI CGRYCG 1 cut(s) 372
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 6 cut(s) 204, 242, 697, 717, 741, 864
BslI CCNNNNNNNGG 4 cut(s) 367, 383, 709, 863
BsmAI GTCTC 5 cut(s) 46, 297, 438, 492, 646
BsnI GGCC 5 cut(s) 119, 144, 585, 701, 721
Bso31I GGTCTC 3 cut(s) 297, 492, 646
BsoBI CYCGRG 1 cut(s) 241
Bsp120I GGGCCC 1 cut(s) 699
Bsp1286I GDGCHC 1 cut(s) 703
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 3 cut(s) 174, 670, 850
BspACI CCGC 2 cut(s) 178, 767
BspANI GGCC 5 cut(s) 119, 144, 585, 701, 721
BspCNI CTCAG 2 cut(s) 56, 322
BspLI GGNNCC 6 cut(s) 6, 154, 701, 715, 787, 862
BspMAI CTGCAG 1 cut(s) 40
BspPI GGATC 2 cut(s) 169, 845
BspT107I GGYRCC 4 cut(s) 4, 152, 713, 785
BspTNI GGTCTC 3 cut(s) 297, 492, 646
BsrDI GCAATG 1 cut(s) 558
BsrFI RCCGGY 1 cut(s) 203
BsrGI TGTACA 1 cut(s) 897
BsrI ACTGG 1 cut(s) 479
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 5 cut(s) 114, 241, 592, 789, 856
BssMI GATC 3 cut(s) 174, 670, 850
BssNI GRCGYC 2 cut(s) 5, 198
BssT1I CCWWGG 1 cut(s) 592
Bst2UI CCWGG 6 cut(s) 116, 315, 791, 805, 815, 858
Bst4CI ACNGT 2 cut(s) 370, 463
Bst6I CTCTTC 2 cut(s) 74, 279
BstACI GRCGYC 2 cut(s) 5, 198
BstAUI TGTACA 1 cut(s) 897
BstBAI YACGTR 1 cut(s) 400
BstDEI CTNAG 2 cut(s) 43, 330
BstF5I GGATG 1 cut(s) 760
BstH2I RGCGCY 2 cut(s) 8, 27
BstHHI GCGC 2 cut(s) 7, 26
BstKTI GATC 3 cut(s) 177, 673, 853
BstMAI GTCTC 5 cut(s) 46, 297, 438, 492, 646
BstMBI GATC 3 cut(s) 174, 670, 850
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 6 cut(s) 32, 186, 371, 455, 536, 764
BstNI CCWGG 6 cut(s) 116, 315, 791, 805, 815, 858
BstPAI GACNNNNGTC 1 cut(s) 481
BstSFI CTRYAG 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 703
BstV1I GCAGC 3 cut(s) 22, 406, 782
BstX2I RGATCY 1 cut(s) 850
BstXI CCANNNNNNTGG 2 cut(s) 593, 620
BstYI RGATCY 1 cut(s) 850
BsuRI GGCC 5 cut(s) 119, 144, 585, 701, 721
BtsCI GGATG 1 cut(s) 760
BtsIMutI CAGTG 3 cut(s) 51, 327, 486
CfoI GCGC 2 cut(s) 7, 26
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 3 cut(s) 699, 700, 719
Cfr9I CCCGGG 1 cut(s) 241
CseI GACGC 2 cut(s) 206, 425
Csp6I GTAC 3 cut(s) 714, 786, 898
CviQI GTAC 3 cut(s) 714, 786, 898
DdeI CTNAG 2 cut(s) 43, 330
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 176, 672, 852
DpnII GATC 3 cut(s) 174, 670, 850
DraIII CACNNNGTG 1 cut(s) 508
EaeI YGGCCR 1 cut(s) 583
Eam1104I CTCTTC 2 cut(s) 74, 279
EarI CTCTTC 2 cut(s) 74, 279
Eco130I CCWWGG 1 cut(s) 592
Eco24I GRGCYC 1 cut(s) 703
Eco31I GGTCTC 3 cut(s) 297, 492, 646
Eco57I CTGAAG 1 cut(s) 936
Eco72I CACGTG 1 cut(s) 400
Eco88I CYCGRG 1 cut(s) 241
EcoRII CCWGG 6 cut(s) 114, 313, 789, 803, 813, 856
EcoT14I CCWWGG 1 cut(s) 592
EcoT22I ATGCAT 1 cut(s) 760
EcoT38I GRGCYC 1 cut(s) 703
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 592
FaiI YATR 7 cut(s) 356, 384, 407, 561, 686, 708, 871
FbaI TGATCA 1 cut(s) 670
Fnu4HI GCNGC 4 cut(s) 36, 420, 768, 771
FokI GGATG 1 cut(s) 767
FriOI GRGCYC 1 cut(s) 703
Fsp4HI GCNGC 4 cut(s) 36, 420, 768, 771
FspBI CTAG 2 cut(s) 519, 903
GlaI GCGC 2 cut(s) 6, 25
GluI GCNGC 4 cut(s) 36, 420, 768, 771
GsuI CTGGAG 3 cut(s) 297, 321, 797
HaeII RGCGCY 2 cut(s) 8, 27
HaeIII GGCC 5 cut(s) 119, 144, 585, 701, 721
HapII CCGG 6 cut(s) 204, 242, 697, 717, 741, 864
HgaI GACGC 2 cut(s) 206, 425
HhaI GCGC 2 cut(s) 7, 26
Hin1I GRCGYC 2 cut(s) 5, 198
Hin6I GCGC 2 cut(s) 5, 24
HinP1I GCGC 2 cut(s) 5, 24
HinfI GANTC 2 cut(s) 214, 296
HpaII CCGG 6 cut(s) 204, 242, 697, 717, 741, 864
Hpy166II GTNNAC 1 cut(s) 900
Hpy188I TCNGA 2 cut(s) 174, 196
Hpy188III TCNNGA 2 cut(s) 300, 844
Hpy8I GTNNAC 1 cut(s) 900
Hpy99I CGWCG 1 cut(s) 528
HpyAV CCTTC 2 cut(s) 911, 918
HpyCH4III ACNGT 2 cut(s) 370, 463
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 4 cut(s) 38, 341, 390, 758
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 186, 371, 455, 536, 764
HpyF3I CTNAG 2 cut(s) 43, 330
HpySE526I ACGT 1 cut(s) 399
Hsp92I GRCGYC 2 cut(s) 5, 198
HspAI GCGC 2 cut(s) 5, 24
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 2 cut(s) 717, 789
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 3 cut(s) 174, 670, 850
LmnI GCTCC 3 cut(s) 316, 379, 455
Lsp1109I GCAGC 3 cut(s) 22, 406, 782
LweI GCATC 2 cut(s) 189, 745
MaeI CTAG 2 cut(s) 519, 903
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 4 cut(s) 325, 647, 659, 880
MalI GATC 3 cut(s) 176, 672, 852
MboI GATC 3 cut(s) 174, 670, 850
MboII GAAGA 4 cut(s) 61, 136, 296, 832
MflI RGATCY 1 cut(s) 850
MhlI GDGCHC 1 cut(s) 703
MlsI TGGCCA 1 cut(s) 585
MluCI AATT 4 cut(s) 276, 486, 545, 726
MluNI TGGCCA 1 cut(s) 585
Mly113I GGCGCC 1 cut(s) 5
Mox20I TGGCCA 1 cut(s) 585
Mph1103I ATGCAT 1 cut(s) 760
MscI TGGCCA 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 138
Msp20I TGGCCA 1 cut(s) 585
MspI CCGG 6 cut(s) 204, 242, 697, 717, 741, 864
MvaI CCWGG 6 cut(s) 116, 315, 791, 805, 815, 858
MwoI GCNNNNNNNGC 6 cut(s) 32, 186, 371, 455, 536, 764
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 4 cut(s) 242, 243, 698, 718
NdeII GATC 3 cut(s) 174, 670, 850
NlaIV GGNNCC 6 cut(s) 6, 154, 701, 715, 787, 862
NmuCI GTSAC 1 cut(s) 325
NsiI ATGCAT 1 cut(s) 760
PfeI GAWTC 2 cut(s) 214, 296
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 4 cut(s) 37, 421, 769, 772
PluTI GGCGCC 1 cut(s) 8
PmaCI CACGTG 1 cut(s) 400
PmlI CACGTG 1 cut(s) 400
Ppu21I YACGTR 1 cut(s) 400
PshAI GACNNNNGTC 1 cut(s) 481
Psp6I CCWGG 6 cut(s) 114, 313, 789, 803, 813, 856
PspCI CACGTG 1 cut(s) 400
PspGI CCWGG 6 cut(s) 114, 313, 789, 803, 813, 856
PspN4I GGNNCC 6 cut(s) 6, 154, 701, 715, 787, 862
PspOMI GGGCCC 1 cut(s) 699
PspPI GGNCC 3 cut(s) 699, 700, 719
PsrI GAACNNNNNNTAC 2 cut(s) 769, 801
PstI CTGCAG 1 cut(s) 40
PsuI RGATCY 1 cut(s) 850
RsaI GTAC 3 cut(s) 715, 787, 899
RsaNI GTAC 3 cut(s) 714, 786, 898
RseI CAYNNNNRTG 1 cut(s) 138
SatI GCNGC 4 cut(s) 36, 420, 768, 771
Sau3AI GATC 3 cut(s) 174, 670, 850
Sau96I GGNCC 3 cut(s) 699, 700, 719
SduI GDGCHC 1 cut(s) 703
SetI ASST 9 cut(s) 309, 319, 355, 402, 412, 451, 658, 784, 809
SfaNI GCATC 2 cut(s) 189, 745
SfcI CTRYAG 1 cut(s) 36
SfoI GGCGCC 1 cut(s) 6
SmaI CCCGGG 1 cut(s) 243
SmiMI CAYNNNNRTG 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 643
SmoI CTYRAG 1 cut(s) 643
Sse9I AATT 4 cut(s) 276, 486, 545, 726
SsiI CCGC 2 cut(s) 178, 767
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 514
SspMI CTAG 2 cut(s) 519, 903
StyI CCWWGG 1 cut(s) 592
TaaI ACNGT 2 cut(s) 370, 463
TaiI ACGT 1 cut(s) 402
TaqI TCGA 1 cut(s) 122
TasI AATT 4 cut(s) 276, 486, 545, 726
TatI WGTACW 1 cut(s) 897
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 2 cut(s) 214, 296
TscAI CASTG 3 cut(s) 51, 334, 486
TseFI GTSAC 1 cut(s) 325
TseI GCWGC 3 cut(s) 35, 419, 770
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 4 cut(s) 558, 569, 585, 593
TspGWI ACGGA 1 cut(s) 548
TspMI CCCGGG 1 cut(s) 241
TspRI CASTG 3 cut(s) 51, 334, 486
XapI RAATTY 1 cut(s) 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
XmaI CCCGGG 1 cut(s) 241
XspI CTAG 2 cut(s) 519, 903
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.