RLG00000034902

cysteine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014007
Physical Location & Seq
Reverse (-)
56792658 .. 56797242
4585 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034902

Sequence Viewer

Length: 1578 bp
ATGCCAATGCAGGGGAGTGTAGGGGCATCATCTTTTGGCACCGCAAGCAATGGCATGGTTCTTACTGGTATGAAGGCTAGGGTTTCTACCCCGTGCCCCTCTTTGGTGTTTCGGACACCAATGCCTGGTCCAGGCGAAGAGCCCTCGTTGAGTGCCTTGTTTTCGAAGCAAAAGCGACAGGTGATGGTGCGTGACCTCCAAGAAACCGCCGGAACTCCAGAGCACCTAACTGGCAAACGTCGTGGACGTGAAGATGGATTGACCATATCCTCTAAGAAGCTGAAGTTGAGTTTGGCTGTGGTTCGAACCGCCGGAATGGCGCCTGTGAGAATTACAGGCGCTATGGTCGCTGCAATCTCAGTGACCATGGCCTTGCTCTCTGTCGTCCTCTACAAGTCCAAGAAGACCAAACCGGCCTTACCCTCAAACAAAAATCCCAGAAATGGCCTCGTTGGAGCCATTGGCAACACCCCTCTCATTCGCATCAACAGCCTCTCTGACGCCACTGGTTGTGAAATACTTGGGAAGTGCGAGTTTTTGAACCCAGGAGGGAGTGTGAAAGATAGAGTTGCTGTGAAAATTATTGAAGAGGCTTTGGAATCTGGAGACCTAGCTCCAGGTGGAATAGTCACTGAGGGAAGTGCTGGAAGCACAGCTATTAGCCTTGCTACAGTTGCTCCTGCTTATGGTTGCAAATGCCATGTGGTTATACCTGATGACGCTGCTATTGAGAAGTCTCAGATACTTGAAGCCCTAGGAGCCACTGTTGAGAGGGTAAGGCCAGTTTCAATCACACACAGGGATCACTTTGTCAACATTGCTAGGCGACGGGCATCAGAAGCAAATGAGTTAGCTGCAAAGCATAGAAAAGATGAGCAAAAGAATGGCAAAACCCTTGACCAAGTTAATGGTTTCCATTATGGTGAAGAGAAACAGTTGTTTCCAAGGAATTGTCCTGGTGGATACTTTGCTGATCAATTTGAAAACTTGGCAAACTTCCGGGCACACTATGAGGGTACCGGGCCTGAGATTTGGGAGCAGACTGGAGGAAATGTAGATGCATTTGTGGCGGCAGCAGGAACAGGTGGTACTGTTGCTGGTGCTTCCAAGTTTCTACAGGAAAGGAATCCAAACATCAAATGCTTCCTGATAGACCCTCCTGGTTCTGGTTTATTTAATAAGATTACGAGGGGAGTGATGTACAGCCAAGAGGAGGCTGAAGGGCGAAGGCTAAAGAATCCATTTGACACAATAACTGAAGGAATTGGAATCAATAGACTAACAAAGAATTTTCTGATGGCAAAGCTTGATGGGGCTTTCCGAGGCACAGATAAGGAAGCTGTTGAAATGTCCAGGTTTCTGATGAAGAACGATGGGCTCTTCTTGGGGAGTTCTTCAGCTATGAATTGTGTTGGAGCAGTTCGAGTGGCACGGTCAATTGGTCCTGGTCACACTATTGTCACCATATTGTGTGATAGTGGGTTGAGACATCTGAGCAAATTTTACAATGCTGAGTATCTGTCTAAATTTGATTTGACACCCACTGCTTGCGGATTAGAGTTCCTGCGTGTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

526

Amino Acids

56.08

Weight (kDa)

9.22

Isoelectric Point (pI)

38.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 153 - 492 3.6e-65 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55880 AT1G55880
fragaria_vesca FvH4_3g29380 FvH4_3g29380
malus_domestica MD03G1184200.v1.1
prunus_persica Prupe.4G242200_v2.0.a1
pyrus_communis pycom03g13750
rosa_chinensis RchiOBHm_Chr5g0053771
rosa_laevigata RLG00000034902
rosa_multiflora Rmu_sc0001759.1_g000024
rosa_roxburghii Rroxscaffold_1G00026820
rosa_rugosa Rorug05G0282500 Rorug05G0282600 Rorug05G0282700
rosa_samantha Rh5BG364700 Rh5DG379500
rosa_wichuraiana Rw5G033350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1016
AccB1I GGYRCC 3 cut(s) 38, 319, 1016
AccB7I CCANNNNNTGG 1 cut(s) 125
AciI CCGC 5 cut(s) 42, 207, 309, 1070, 1551
AclWI GGATC 1 cut(s) 810
AcsI RAATTY 3 cut(s) 1288, 1499, 1526
AcuI CTGAAG 4 cut(s) 302, 1239, 1278, 1380
AcyI GRCGYC 2 cut(s) 320, 501
AfaI GTAC 3 cut(s) 1018, 1090, 1202
AfiI CCNNNNNNNGG 8 cut(s) 11, 103, 125, 131, 443, 686, 1166, 1213
AgsI TTSAA 6 cut(s) 541, 587, 749, 789, 983, 1346
AjiI CACGTC 1 cut(s) 248
AjnI CCWGG 8 cut(s) 124, 130, 544, 616, 955, 1159, 1352, 1444
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AluBI AGCT 7 cut(s) 280, 614, 656, 854, 1306, 1340, 1400
AluI AGCT 7 cut(s) 280, 614, 656, 854, 1306, 1340, 1400
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 3 cut(s) 600, 741, 1480
AlwI GGATC 1 cut(s) 810
AoxI GGCC 5 cut(s) 369, 414, 445, 779, 1022
ApeKI GCWGC 4 cut(s) 350, 722, 854, 1073
ApoI RAATTY 3 cut(s) 1288, 1499, 1526
Asp718I GGTACC 1 cut(s) 1016
AspA2I CCTAGG 1 cut(s) 754
AspLEI GCGC 2 cut(s) 322, 341
AspS9I GGNCC 3 cut(s) 128, 1022, 1442
AsuC2I CCSGG 2 cut(s) 1001, 1021
AsuHPI GGTGA 3 cut(s) 193, 935, 1453
AsuII TTCGAA 2 cut(s) 164, 304
AvaII GGWCC 2 cut(s) 128, 1442
AvrII CCTAGG 1 cut(s) 754
BaeGI GKGCMC 2 cut(s) 98, 1006
BanI GGYRCC 3 cut(s) 38, 319, 1016
BanII GRGCYC 2 cut(s) 144, 1380
BbsI GAAGAC 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 4 cut(s) 337, 709, 841, 1085
BccI CCATC 5 cut(s) 178, 248, 1291, 1304, 1367
BciT130I CCWGG 8 cut(s) 126, 132, 546, 618, 957, 1161, 1354, 1446
BciVI GTATCC 1 cut(s) 956
BclI TGATCA 1 cut(s) 973
BcnI CCSGG 2 cut(s) 1001, 1021
BcoDI GTCTC 3 cut(s) 600, 741, 1480
BfaI CTAG 4 cut(s) 78, 611, 755, 822
BfmI CTRYAG 2 cut(s) 669, 1115
BfoI RGCGCY 2 cut(s) 323, 342
BfuI GTATCC 1 cut(s) 956
BglI GCCNNNNNGGC 1 cut(s) 317
BisI GCNGC 5 cut(s) 351, 723, 855, 1071, 1074
BlnI CCTAGG 1 cut(s) 754
BlsI GCNGC 5 cut(s) 352, 724, 856, 1072, 1075
Bme18I GGWCC 2 cut(s) 128, 1442
BmgBI CACGTC 1 cut(s) 248
BmgT120I GGNCC 3 cut(s) 128, 1022, 1442
BmiI GGNNCC 5 cut(s) 40, 321, 457, 760, 1018
BmsI GCATC 4 cut(s) 35, 492, 842, 1048
BpiI GAAGAC 1 cut(s) 410
BpmI CTGGAG 4 cut(s) 201, 600, 624, 1065
Bpu14I TTCGAA 2 cut(s) 164, 304
BpuMI CCSGG 2 cut(s) 1001, 1021
BsaHI GRCGYC 2 cut(s) 320, 501
BsaI GGTCTC 1 cut(s) 600
BsaJI CCNNGG 5 cut(s) 366, 544, 754, 944, 1321
BsaXI ACNNNNNCTCC 8 cut(s) 540, 570, 661, 691, 750, 780, 1038, 1068
Bsc4I CCNNNNNNNGG 8 cut(s) 11, 103, 125, 131, 443, 686, 1166, 1213
Bse118I RCCGGY 1 cut(s) 412
Bse1I ACTGG 5 cut(s) 70, 235, 511, 782, 1048
Bse3DI GCAATG 2 cut(s) 55, 816
BseBI CCWGG 8 cut(s) 126, 132, 546, 618, 957, 1161, 1354, 1446
BseDI CCNNGG 5 cut(s) 366, 544, 754, 944, 1321
BseLI CCNNNNNNNGG 8 cut(s) 11, 103, 125, 131, 443, 686, 1166, 1213
BseMI GCAATG 2 cut(s) 55, 816
BseMII CTCAG 6 cut(s) 372, 624, 752, 1017, 1484, 1503
BseNI ACTGG 5 cut(s) 70, 235, 511, 782, 1048
BseRI GAGGAG 1 cut(s) 1226
BseSI GKGCMC 2 cut(s) 98, 1006
BseXI GCAGC 4 cut(s) 337, 709, 841, 1085
BshFI GGCC 5 cut(s) 371, 416, 447, 781, 1024
BshNI GGYRCC 3 cut(s) 38, 319, 1016
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 5 cut(s) 210, 312, 413, 1000, 1020
BslI CCNNNNNNNGG 8 cut(s) 11, 103, 125, 131, 443, 686, 1166, 1213
BsmAI GTCTC 3 cut(s) 600, 741, 1480
BsnI GGCC 5 cut(s) 371, 416, 447, 781, 1024
Bso31I GGTCTC 1 cut(s) 600
Bsp119I TTCGAA 2 cut(s) 164, 304
Bsp1286I GDGCHC 5 cut(s) 98, 144, 225, 1006, 1380
Bsp1407I TGTACA 1 cut(s) 1200
Bsp143I GATC 2 cut(s) 802, 973
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 5 cut(s) 42, 207, 309, 1070, 1551
BspANI GGCC 5 cut(s) 371, 416, 447, 781, 1024
BspCNI CTCAG 6 cut(s) 371, 625, 751, 1018, 1485, 1504
BspLI GGNNCC 5 cut(s) 40, 321, 457, 760, 1018
BspPI GGATC 1 cut(s) 810
BspQI GCTCTTC 2 cut(s) 132, 1385
BspT104I TTCGAA 2 cut(s) 164, 304
BspT107I GGYRCC 3 cut(s) 38, 319, 1016
BspTNI GGTCTC 1 cut(s) 600
BsrDI GCAATG 2 cut(s) 55, 816
BsrFI RCCGGY 1 cut(s) 412
BsrGI TGTACA 1 cut(s) 1200
BsrI ACTGG 5 cut(s) 70, 235, 511, 782, 1048
BssAI RCCGGY 1 cut(s) 412
BssECI CCNNGG 5 cut(s) 366, 544, 754, 944, 1321
BssMI GATC 2 cut(s) 802, 973
BssNI GRCGYC 2 cut(s) 320, 501
BssT1I CCWWGG 3 cut(s) 366, 754, 944
Bst2UI CCWGG 8 cut(s) 126, 132, 546, 618, 957, 1161, 1354, 1446
Bst4CI ACNGT 5 cut(s) 673, 766, 936, 1093, 1434
Bst6I CTCTTC 4 cut(s) 132, 582, 921, 1385
BstACI GRCGYC 2 cut(s) 320, 501
BstAUI TGTACA 1 cut(s) 1200
BstBI TTCGAA 2 cut(s) 164, 304
BstC8I GCNNGC 2 cut(s) 46, 1549
BstDEI CTNAG 7 cut(s) 273, 358, 633, 738, 1026, 1493, 1512
BstDSI CCRYGG 1 cut(s) 366
BstENI CCTNNNNNAGG 1 cut(s) 129
BstH2I RGCGCY 2 cut(s) 323, 342
BstHHI GCGC 2 cut(s) 322, 341
BstKTI GATC 2 cut(s) 805, 976
BstMAI GTCTC 3 cut(s) 600, 741, 1480
BstMBI GATC 2 cut(s) 802, 973
BstMWI GCNNNNNNNGC 8 cut(s) 45, 317, 347, 489, 674, 758, 839, 1067
BstNI CCWGG 8 cut(s) 126, 132, 546, 618, 957, 1161, 1354, 1446
BstSFI CTRYAG 2 cut(s) 669, 1115
BstSLI GKGCMC 2 cut(s) 98, 1006
BstV1I GCAGC 4 cut(s) 337, 709, 841, 1085
BstV2I GAAGAC 1 cut(s) 410
BstXI CCANNNNNNTGG 1 cut(s) 908
BsuI GTATCC 1 cut(s) 956
BsuRI GGCC 5 cut(s) 371, 416, 447, 781, 1024
BtgI CCRYGG 1 cut(s) 366
BtrI CACGTC 1 cut(s) 248
BtsI GCAGTG 1 cut(s) 1542
BtsIMutI CAGTG 5 cut(s) 366, 504, 630, 762, 1542
Cac8I GCNNGC 2 cut(s) 46, 1549
CfoI GCGC 2 cut(s) 322, 341
Cfr10I RCCGGY 1 cut(s) 412
Cfr13I GGNCC 3 cut(s) 128, 1022, 1442
CseI GACGC 2 cut(s) 509, 728
Csp6I GTAC 3 cut(s) 1017, 1089, 1201
CspCI CAANNNNNGTGG 2 cut(s) 223, 258
CviAII CATG 3 cut(s) 55, 367, 701
CviQI GTAC 3 cut(s) 1017, 1089, 1201
DdeI CTNAG 7 cut(s) 273, 358, 633, 738, 1026, 1493, 1512
DinI GGCGCC 1 cut(s) 321
DpnI GATC 2 cut(s) 804, 975
DpnII GATC 2 cut(s) 802, 973
Eam1104I CTCTTC 4 cut(s) 132, 582, 921, 1385
EarI CTCTTC 4 cut(s) 132, 582, 921, 1385
Eco130I CCWWGG 3 cut(s) 366, 754, 944
Eco24I GRGCYC 2 cut(s) 144, 1380
Eco31I GGTCTC 1 cut(s) 600
Eco47I GGWCC 2 cut(s) 128, 1442
Eco57I CTGAAG 4 cut(s) 302, 1239, 1278, 1380
EcoNI CCTNNNNNAGG 1 cut(s) 129
EcoRII CCWGG 8 cut(s) 124, 130, 544, 616, 955, 1159, 1352, 1444
EcoT14I CCWWGG 3 cut(s) 366, 754, 944
EcoT22I ATGCAT 1 cut(s) 1063
EcoT38I GRGCYC 2 cut(s) 144, 1380
EgeI GGCGCC 1 cut(s) 321
EheI GGCGCC 1 cut(s) 321
ErhI CCWWGG 3 cut(s) 366, 754, 944
FaeI CATG 3 cut(s) 58, 370, 704
FatI CATG 3 cut(s) 54, 366, 700
FbaI TGATCA 1 cut(s) 973
Fnu4HI GCNGC 5 cut(s) 351, 723, 855, 1071, 1074
FriOI GRGCYC 2 cut(s) 144, 1380
Fsp4HI GCNGC 5 cut(s) 351, 723, 855, 1071, 1074
FspBI CTAG 4 cut(s) 78, 611, 755, 822
GlaI GCGC 2 cut(s) 321, 340
GluI GCNGC 5 cut(s) 351, 723, 855, 1071, 1074
GsuI CTGGAG 4 cut(s) 201, 600, 624, 1065
HaeII RGCGCY 2 cut(s) 323, 342
HaeIII GGCC 5 cut(s) 371, 416, 447, 781, 1024
HapII CCGG 5 cut(s) 210, 312, 413, 1000, 1020
HgaI GACGC 2 cut(s) 509, 728
HhaI GCGC 2 cut(s) 322, 341
Hin1I GRCGYC 2 cut(s) 320, 501
Hin1II CATG 3 cut(s) 58, 370, 704
Hin6I GCGC 2 cut(s) 320, 339
HinP1I GCGC 2 cut(s) 320, 339
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HindIII AAGCTT 1 cut(s) 1304
HinfI GANTC 4 cut(s) 599, 1126, 1237, 1269
HpaII CCGG 5 cut(s) 210, 312, 413, 1000, 1020
HphI GGTGA 3 cut(s) 193, 935, 1453
Hpy166II GTNNAC 2 cut(s) 245, 814
Hpy188I TCNGA 8 cut(s) 114, 499, 741, 838, 1296, 1322, 1362, 1494
Hpy188III TCNNGA 3 cut(s) 218, 603, 1147
Hpy8I GTNNAC 2 cut(s) 245, 814
Hpy99I CGWCG 2 cut(s) 243, 831
HpyAV CCTTC 4 cut(s) 67, 1214, 1221, 1253
HpyCH4III ACNGT 5 cut(s) 673, 766, 936, 1093, 1434
HpyCH4IV ACGT 2 cut(s) 238, 247
HpyCH4V TGCA 5 cut(s) 10, 353, 693, 857, 1061
HpyF10VI GCNNNNNNNGC 8 cut(s) 45, 317, 347, 489, 674, 758, 839, 1067
HpyF3I CTNAG 7 cut(s) 273, 358, 633, 738, 1026, 1493, 1512
HpySE526I ACGT 2 cut(s) 238, 247
Hsp92I GRCGYC 2 cut(s) 320, 501
Hsp92II CATG 3 cut(s) 58, 370, 704
HspAI GCGC 2 cut(s) 320, 339
KasI GGCGCC 1 cut(s) 319
KpnI GGTACC 1 cut(s) 1020
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 2 cut(s) 802, 973
LguI GCTCTTC 2 cut(s) 132, 1385
LmnI GCTCC 6 cut(s) 455, 619, 682, 758, 1036, 1415
Lsp1109I GCAGC 4 cut(s) 337, 709, 841, 1085
LweI GCATC 4 cut(s) 35, 492, 842, 1048
MaeI CTAG 4 cut(s) 78, 611, 755, 822
MaeII ACGT 2 cut(s) 238, 247
MaeIII GTNAC 5 cut(s) 191, 361, 628, 1448, 1459
MalI GATC 2 cut(s) 804, 975
MboI GATC 2 cut(s) 802, 973
MboII GAAGA 8 cut(s) 149, 263, 415, 599, 938, 1372, 1378, 1386
MfeI CAATTG 1 cut(s) 1437
MhlI GDGCHC 5 cut(s) 98, 144, 225, 1006, 1380
Mly113I GGCGCC 1 cut(s) 320
MmeI TCCRAC 3 cut(s) 433, 1393, 1552
Mph1103I ATGCAT 1 cut(s) 1063
MseI TTAA 2 cut(s) 906, 1176
MslI CAYNNNNRTG 1 cut(s) 921
MspI CCGG 5 cut(s) 210, 312, 413, 1000, 1020
MunI CAATTG 1 cut(s) 1437
MvaI CCWGG 8 cut(s) 126, 132, 546, 618, 957, 1161, 1354, 1446
MwoI GCNNNNNNNGC 8 cut(s) 45, 317, 347, 489, 674, 758, 839, 1067
NarI GGCGCC 1 cut(s) 320
NciI CCSGG 2 cut(s) 1001, 1021
NcoI CCATGG 1 cut(s) 366
NdeII GATC 2 cut(s) 802, 973
NlaIII CATG 3 cut(s) 58, 370, 704
NlaIV GGNNCC 5 cut(s) 40, 321, 457, 760, 1018
NmuCI GTSAC 5 cut(s) 191, 361, 628, 1448, 1459
NsiI ATGCAT 1 cut(s) 1063
NspV TTCGAA 2 cut(s) 164, 304
PciSI GCTCTTC 2 cut(s) 132, 1385
PcsI WCGNNNNNNNCGW 1 cut(s) 244
PfeI GAWTC 4 cut(s) 599, 1126, 1237, 1269
PflMI CCANNNNNTGG 1 cut(s) 125
PkrI GCNGC 5 cut(s) 352, 724, 856, 1072, 1075
PluTI GGCGCC 1 cut(s) 323
Psp6I CCWGG 8 cut(s) 124, 130, 544, 616, 955, 1159, 1352, 1444
PspGI CCWGG 8 cut(s) 124, 130, 544, 616, 955, 1159, 1352, 1444
PspN4I GGNNCC 5 cut(s) 40, 321, 457, 760, 1018
PspPI GGNCC 3 cut(s) 128, 1022, 1442
PsrI GAACNNNNNNTAC 2 cut(s) 1072, 1104
RsaI GTAC 3 cut(s) 1018, 1090, 1202
RsaNI GTAC 3 cut(s) 1017, 1089, 1201
RseI CAYNNNNRTG 1 cut(s) 921
SapI GCTCTTC 2 cut(s) 132, 1385
SaqAI TTAA 2 cut(s) 906, 1176
SatI GCNGC 5 cut(s) 351, 723, 855, 1071, 1074
Sau3AI GATC 2 cut(s) 802, 973
Sau96I GGNCC 3 cut(s) 128, 1022, 1442
SduI GDGCHC 5 cut(s) 98, 144, 225, 1006, 1380
SfaNI GCATC 4 cut(s) 35, 492, 842, 1048
SfcI CTRYAG 2 cut(s) 669, 1115
SfoI GGCGCC 1 cut(s) 321
SfuI TTCGAA 2 cut(s) 164, 304
SinI GGWCC 2 cut(s) 128, 1442
SmiMI CAYNNNNRTG 1 cut(s) 921
SsiI CCGC 5 cut(s) 42, 207, 309, 1070, 1551
SspDI GGCGCC 1 cut(s) 319
SspMI CTAG 4 cut(s) 78, 611, 755, 822
StyI CCWWGG 3 cut(s) 366, 754, 944
TaaI ACNGT 5 cut(s) 673, 766, 936, 1093, 1434
TaiI ACGT 2 cut(s) 241, 250
TaqI TCGA 3 cut(s) 164, 304, 1423
TatI WGTACW 1 cut(s) 1200
TauI GCSGC 1 cut(s) 1073
TfiI GAWTC 4 cut(s) 599, 1126, 1237, 1269
Tru1I TTAA 2 cut(s) 906, 1176
Tru9I TTAA 2 cut(s) 906, 1176
TscAI CASTG 5 cut(s) 366, 511, 637, 769, 1549
TseFI GTSAC 5 cut(s) 191, 361, 628, 1448, 1459
TseI GCWGC 4 cut(s) 350, 722, 854, 1073
Tsp45I GTSAC 5 cut(s) 191, 361, 628, 1448, 1459
TspDTI ATGAA 3 cut(s) 86, 1379, 1418
TspRI CASTG 5 cut(s) 366, 511, 637, 769, 1549
Van91I CCANNNNNTGG 1 cut(s) 125
VpaK11BI GGWCC 2 cut(s) 128, 1442
XagI CCTNNNNNAGG 1 cut(s) 129
XapI RAATTY 3 cut(s) 1288, 1499, 1526
XmaJI CCTAGG 1 cut(s) 754
XspI CTAG 4 cut(s) 78, 611, 755, 822
Zsp2I ATGCAT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.