Rorug05G0282700

cysteine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0014007
Physical Location & Seq
Reverse (-)
32341062 .. 32343567
2506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0282700.1

Sequence Viewer

Length: 1200 bp
ATGGCACATAGTGGTATTAATGACATCAATGGCACTGCCATGTACGGCGAGGGAAACCCCTCGGAATTGAGCTGCGTATTTATGGAGGGCAATAATATGGGTCTGAAGAAAGGTCCATGGACGGCGGTGGAGGATCGAATTCTGATGGAGTACGTGAGCAAACAGGGCGAAGGGAACTGGAATTCGGTGCAATGGAATTCCGGGCTCAAGCGTTGCGGCAAGAGTTGCAGGCTCCGGTGGGCTAATCATCTCCGCCCTAATTTGAAGAAAGGTGCATTCACACCCGAGGAAGAGAGACTCATTCTTGAACTCCACGCCAAATACGGCAACAAATGGGCTCGCATGGCCTCTCAGTTACCTGGACGAACAGATAATGAAATTAAAAATTACTGGAACACAAGGGTGAAGAAGCGACTACGCAAAGGTCTTCCACTATATCCTCATGACATTAACCCATCCAAACGAGTATGTCAGTCACATTCTCATTCCCAACAAAACGCAATGAGCATTCCCACAGGAGTAACCCAACTTTCAACCACCACAGTACCACAGACAGACACTAGTTTTAGCTTCCAATTACAAGCCTCATCATCCCAAAACAAAACCCAACTCCCTCCCCTCCCCTCCCAAAACCATCCCAACTCCACTCTCAATCATTCCCTCTCTCTATTTGATCTCACCACCTCGTCCTGCACTACCTCGGTGCCCTCGTTTACCTTCCACCACCCCACTCCAATGCTTGGAACCCCGTTAGGCTACAAACGTTACCAAGAAATTACTGGGTTCTCGCCTCCTATTTCTCCAACTCCACAAAGAAATAGCTCCATTTTGCGAACCAGCTCCATGCCCGACATTGCTTCCTATCAACAGTTTGCATCAGGTACAGGCACCATGCCTGACAACACCTCTTTTCAGTTCCAGCCACAGACTTTCAATGAGTCTTTATTGCCATTGCCCCGTACCAGCACCCAGTTCAATTTCGGGGACAGCTTATTGTCACCAATTAGTTCAGTTTATTCCATGAATTCAGAGCTTCCTTCGAGCCAAGTATCTCATACACCAGAGCCTCCTTCAAGCCAACTATCTCAAGCACCGCAGCTCCCTTCAAGTCAAGCATCTCAAGAACAGTCATTGTCAGAGATCACCACCAGTGTTTTCAAAGGCGACGCCGAGGCGAGCCCCGTGCGCGCTTCGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

399

Amino Acids

44.04

Weight (kDa)

9.02

Isoelectric Point (pI)

56.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 37 - 84 5.1e-13 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 40 - 99 5e-12 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 90 - 133 5.1e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 93 - 141 5.9e-09 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55880 AT1G55880
fragaria_vesca FvH4_3g29380 FvH4_3g29380
malus_domestica MD03G1184200.v1.1
prunus_persica Prupe.4G242200_v2.0.a1
pyrus_communis pycom03g13750
rosa_chinensis RchiOBHm_Chr5g0053771
rosa_laevigata RLG00000034902
rosa_multiflora Rmu_sc0001759.1_g000024
rosa_roxburghii Rroxscaffold_1G00026820
rosa_rugosa Rorug05G0282500 Rorug05G0282600 Rorug05G0282700
rosa_samantha Rh5BG364700 Rh5DG379500
rosa_wichuraiana Rw5G033350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 703, 887
AccB7I CCANNNNNTGG 1 cut(s) 740
AccII CGCG 1 cut(s) 1188
AciI CCGC 4 cut(s) 125, 216, 253, 1094
AclI AACGTT 1 cut(s) 763
AclWI GGATC 1 cut(s) 141
AcsI RAATTY 4 cut(s) 138, 181, 196, 1024
AcuI CTGAAG 1 cut(s) 125
AcyI GRCGYC 1 cut(s) 1167
AdeI CACNNNGTG 1 cut(s) 11
AfaI GTAC 5 cut(s) 44, 152, 546, 883, 961
AfiI CCNNNNNNNGG 1 cut(s) 740
AgsI TTSAA 8 cut(s) 265, 308, 534, 934, 976, 1074, 1107, 1159
AhlI ACTAGT 1 cut(s) 560
AjnI CCWGG 1 cut(s) 358
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 7 cut(s) 72, 570, 822, 840, 990, 1033, 1099
AluI AGCT 7 cut(s) 72, 570, 822, 840, 990, 1033, 1099
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 1 cut(s) 141
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 2 cut(s) 72, 1096
ApoI RAATTY 4 cut(s) 138, 181, 196, 1024
AseI ATTAAT 1 cut(s) 18
AspLEI GCGC 2 cut(s) 1188, 1190
AspS9I GGNCC 1 cut(s) 113
AsuC2I CCSGG 1 cut(s) 202
AsuHPI GGTGA 4 cut(s) 415, 670, 990, 1135
AvaI CYCGRG 1 cut(s) 284
AvaII GGWCC 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 708
BaeI ACNNNNGTAYC 2 cut(s) 528, 561
BanI GGYRCC 2 cut(s) 703, 887
BanII GRGCYC 3 cut(s) 207, 340, 1181
BbsI GAAGAC 1 cut(s) 419
BbvI GCAGC 2 cut(s) 59, 1108
BccI CCATC 3 cut(s) 139, 463, 642
BceAI ACGGC 3 cut(s) 61, 138, 340
BcgI CGANNNNNNTGC 2 cut(s) 1165, 1199
BciT130I CCWGG 1 cut(s) 360
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 1 cut(s) 289
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 1 cut(s) 561
BisI GCNGC 3 cut(s) 73, 217, 1097
BlsI GCNGC 3 cut(s) 74, 218, 1098
Bme1390I CCNGG 2 cut(s) 202, 360
Bme18I GGWCC 1 cut(s) 113
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 1 cut(s) 113
BmiI GGNNCC 4 cut(s) 233, 705, 745, 889
BmrFI CCNGG 2 cut(s) 202, 360
BmrI ACTGGG 2 cut(s) 789, 964
BmsI GCATC 2 cut(s) 884, 1124
BmuI ACTGGG 2 cut(s) 789, 964
BpiI GAAGAC 1 cut(s) 419
BpuEI CTTGAG 3 cut(s) 191, 1071, 1104
BpuMI CCSGG 1 cut(s) 202
BsaAI YACGTR 1 cut(s) 154
BsaHI GRCGYC 1 cut(s) 1167
BsaJI CCNNGG 5 cut(s) 60, 116, 285, 699, 1170
BsaWI WCCGGW 1 cut(s) 234
BsaXI ACNNNNNCTCC 2 cut(s) 1083, 1113
Bsc4I CCNNNNNNNGG 1 cut(s) 740
Bse1I ACTGG 5 cut(s) 182, 395, 784, 970, 1149
Bse3DI GCAATG 4 cut(s) 197, 507, 852, 950
BseBI CCWGG 1 cut(s) 360
BseDI CCNNGG 5 cut(s) 60, 116, 285, 699, 1170
BseGI GGATG 3 cut(s) 455, 590, 634
BseLI CCNNNNNNNGG 1 cut(s) 740
BseMI GCAATG 4 cut(s) 197, 507, 852, 950
BseMII CTCAG 1 cut(s) 365
BseNI ACTGG 5 cut(s) 182, 395, 784, 970, 1149
BsePI GCGCGC 1 cut(s) 1186
BseSI GKGCMC 1 cut(s) 708
BseXI GCAGC 2 cut(s) 59, 1108
BsgI GTGCAG 1 cut(s) 676
Bsh1236I CGCG 1 cut(s) 1188
BshFI GGCC 1 cut(s) 347
BshNI GGYRCC 2 cut(s) 703, 887
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 2 cut(s) 201, 235
BslFI GGGAC 1 cut(s) 998
BslI CCNNNNNNNGG 1 cut(s) 740
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 998
BsmI GAATGC 2 cut(s) 275, 507
BsnI GGCC 1 cut(s) 347
BsoBI CYCGRG 1 cut(s) 284
Bsp1286I GDGCHC 4 cut(s) 207, 340, 708, 1181
Bsp143I GATC 3 cut(s) 133, 673, 1140
Bsp19I CCATGG 1 cut(s) 116
BspACI CCGC 4 cut(s) 125, 216, 253, 1094
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 1 cut(s) 364
BspFNI CGCG 1 cut(s) 1188
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 4 cut(s) 233, 705, 745, 889
BspPI GGATC 1 cut(s) 141
BspT107I GGYRCC 2 cut(s) 703, 887
BsrDI GCAATG 4 cut(s) 197, 507, 852, 950
BsrI ACTGG 5 cut(s) 182, 395, 784, 970, 1149
BssECI CCNNGG 5 cut(s) 60, 116, 285, 699, 1170
BssHII GCGCGC 1 cut(s) 1186
BssMI GATC 3 cut(s) 133, 673, 1140
BssNI GRCGYC 1 cut(s) 1167
BssT1I CCWWGG 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 3 cut(s) 544, 870, 1128
Bst6I CTCTTC 1 cut(s) 285
BstACI GRCGYC 1 cut(s) 1167
BstAPI GCANNNNNTGC 1 cut(s) 225
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 4 cut(s) 230, 340, 1177, 1188
BstDEI CTNAG 1 cut(s) 351
BstDSI CCRYGG 1 cut(s) 116
BstF5I GGATG 3 cut(s) 455, 590, 634
BstFNI CGCG 1 cut(s) 1188
BstHHI GCGC 2 cut(s) 1188, 1190
BstKTI GATC 3 cut(s) 136, 676, 1143
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 3 cut(s) 133, 673, 1140
BstMWI GCNNNNNNNGC 4 cut(s) 165, 225, 344, 1185
BstNI CCWGG 1 cut(s) 360
BstSCI CCNGG 2 cut(s) 200, 358
BstSLI GKGCMC 1 cut(s) 708
BstUI CGCG 1 cut(s) 1188
BstV1I GCAGC 2 cut(s) 59, 1108
BstV2I GAAGAC 1 cut(s) 419
BsuRI GGCC 1 cut(s) 347
BtgI CCRYGG 1 cut(s) 116
BtsCI GGATG 3 cut(s) 455, 590, 634
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 2 cut(s) 33, 1156
Cac8I GCNNGC 4 cut(s) 230, 340, 1177, 1188
CciI TCATGA 1 cut(s) 442
CfoI GCGC 2 cut(s) 1188, 1190
Cfr13I GGNCC 1 cut(s) 113
CseI GACGC 1 cut(s) 1175
Csp6I GTAC 5 cut(s) 43, 151, 545, 882, 960
CviAII CATG 7 cut(s) 40, 117, 343, 443, 844, 892, 1021
CviQI GTAC 5 cut(s) 43, 151, 545, 882, 960
DdeI CTNAG 1 cut(s) 351
DpnI GATC 3 cut(s) 135, 675, 1142
DpnII GATC 3 cut(s) 133, 673, 1140
DraIII CACNNNGTG 1 cut(s) 11
Eam1104I CTCTTC 1 cut(s) 285
EarI CTCTTC 1 cut(s) 285
EciI GGCGGA 1 cut(s) 242
Eco130I CCWWGG 1 cut(s) 116
Eco24I GRGCYC 3 cut(s) 207, 340, 1181
Eco47I GGWCC 1 cut(s) 113
Eco57I CTGAAG 1 cut(s) 125
Eco88I CYCGRG 1 cut(s) 284
EcoRI GAATTC 4 cut(s) 138, 181, 196, 1024
EcoRII CCWGG 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 116
EcoT38I GRGCYC 3 cut(s) 207, 340, 1181
ErhI CCWWGG 1 cut(s) 116
FaeI CATG 7 cut(s) 43, 120, 346, 446, 847, 895, 1024
FaqI GGGAC 1 cut(s) 998
FatI CATG 7 cut(s) 39, 116, 342, 442, 843, 891, 1020
Fnu4HI GCNGC 3 cut(s) 73, 217, 1097
FokI GGATG 3 cut(s) 442, 577, 621
FriOI GRGCYC 3 cut(s) 207, 340, 1181
Fsp4HI GCNGC 3 cut(s) 73, 217, 1097
FspBI CTAG 1 cut(s) 561
GlaI GCGC 2 cut(s) 1187, 1189
GluI GCNGC 3 cut(s) 73, 217, 1097
HaeIII GGCC 1 cut(s) 347
HapII CCGG 2 cut(s) 201, 235
HgaI GACGC 1 cut(s) 1175
HhaI GCGC 2 cut(s) 1188, 1190
Hin1I GRCGYC 1 cut(s) 1167
Hin1II CATG 7 cut(s) 43, 120, 346, 446, 847, 895, 1024
Hin6I GCGC 2 cut(s) 1186, 1188
HinP1I GCGC 2 cut(s) 1186, 1188
HinfI GANTC 2 cut(s) 297, 938
HpaII CCGG 2 cut(s) 201, 235
HphI GGTGA 4 cut(s) 415, 670, 990, 1135
Hpy166II GTNNAC 1 cut(s) 714
Hpy188I TCNGA 5 cut(s) 64, 105, 144, 1030, 1138
Hpy188III TCNNGA 3 cut(s) 305, 443, 1121
Hpy8I GTNNAC 1 cut(s) 714
Hpy99I CGWCG 1 cut(s) 1169
HpyAV CCTTC 5 cut(s) 164, 727, 1047, 1080, 1113
HpyCH4III ACNGT 3 cut(s) 544, 870, 1128
HpyCH4IV ACGT 2 cut(s) 153, 763
HpyCH4V TGCA 5 cut(s) 190, 228, 275, 693, 875
HpyF10VI GCNNNNNNNGC 4 cut(s) 165, 225, 344, 1185
HpyF3I CTNAG 1 cut(s) 351
HpySE526I ACGT 2 cut(s) 153, 763
Hsp92I GRCGYC 1 cut(s) 1167
Hsp92II CATG 7 cut(s) 43, 120, 346, 446, 847, 895, 1024
HspAI GCGC 2 cut(s) 1186, 1188
Kzo9I GATC 3 cut(s) 133, 673, 1140
LmnI GCTCC 4 cut(s) 237, 827, 845, 1104
Lsp1109I GCAGC 2 cut(s) 59, 1108
LweI GCATC 2 cut(s) 884, 1124
MaeI CTAG 1 cut(s) 561
MaeII ACGT 2 cut(s) 153, 763
MaeIII GTNAC 5 cut(s) 354, 474, 520, 764, 996
MalI GATC 3 cut(s) 135, 675, 1142
MboI GATC 3 cut(s) 133, 673, 1140
MboII GAAGA 5 cut(s) 118, 277, 302, 418, 419
MhlI GDGCHC 4 cut(s) 207, 340, 708, 1181
MlyI GAGTC 2 cut(s) 291, 947
MmeI TCCRAC 1 cut(s) 827
MseI TTAA 3 cut(s) 18, 381, 450
MslI CAYNNNNRTG 3 cut(s) 38, 401, 734
MspI CCGG 2 cut(s) 201, 235
MspR9I CCNGG 2 cut(s) 202, 360
Mva1269I GAATGC 2 cut(s) 275, 507
MvaI CCWGG 1 cut(s) 360
MvnI CGCG 1 cut(s) 1188
MwoI GCNNNNNNNGC 4 cut(s) 165, 225, 344, 1185
NciI CCSGG 1 cut(s) 202
NcoI CCATGG 1 cut(s) 116
NdeII GATC 3 cut(s) 133, 673, 1140
NlaIII CATG 7 cut(s) 43, 120, 346, 446, 847, 895, 1024
NlaIV GGNNCC 4 cut(s) 233, 705, 745, 889
NmeAIII GCCGAG 1 cut(s) 1195
NmuCI GTSAC 2 cut(s) 474, 996
OliI CACNNNNGTG 1 cut(s) 401
PagI TCATGA 1 cut(s) 442
PauI GCGCGC 1 cut(s) 1186
PcsI WCGNNNNNNNCGW 1 cut(s) 707
PctI GAATGC 2 cut(s) 275, 507
PflMI CCANNNNNTGG 1 cut(s) 740
PkrI GCNGC 3 cut(s) 74, 218, 1098
PleI GAGTC 2 cut(s) 291, 946
PpsI GAGTC 2 cut(s) 291, 946
Ppu21I YACGTR 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 18
Psp1406I AACGTT 1 cut(s) 763
Psp6I CCWGG 1 cut(s) 358
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 4 cut(s) 233, 705, 745, 889
PspPI GGNCC 1 cut(s) 113
PteI GCGCGC 1 cut(s) 1186
RsaI GTAC 5 cut(s) 44, 152, 546, 883, 961
RsaNI GTAC 5 cut(s) 43, 151, 545, 882, 960
RseI CAYNNNNRTG 3 cut(s) 38, 401, 734
SaqAI TTAA 3 cut(s) 18, 381, 450
SatI GCNGC 3 cut(s) 73, 217, 1097
Sau3AI GATC 3 cut(s) 133, 673, 1140
Sau96I GGNCC 1 cut(s) 113
SchI GAGTC 2 cut(s) 291, 947
ScrFI CCNGG 2 cut(s) 202, 360
SduI GDGCHC 4 cut(s) 207, 340, 708, 1181
SfaNI GCATC 2 cut(s) 884, 1124
SinI GGWCC 1 cut(s) 113
SmiMI CAYNNNNRTG 3 cut(s) 38, 401, 734
SmlI CTYRAG 3 cut(s) 206, 1086, 1119
SmoI CTYRAG 3 cut(s) 206, 1086, 1119
SpeI ACTAGT 1 cut(s) 560
SsiI CCGC 4 cut(s) 125, 216, 253, 1094
SspMI CTAG 1 cut(s) 561
StyD4I CCNGG 2 cut(s) 200, 358
StyI CCWWGG 1 cut(s) 116
TaaI ACNGT 3 cut(s) 544, 870, 1128
TaiI ACGT 2 cut(s) 156, 766
TaqI TCGA 2 cut(s) 136, 1040
TauI GCSGC 1 cut(s) 219
Tru1I TTAA 3 cut(s) 18, 381, 450
Tru9I TTAA 3 cut(s) 18, 381, 450
TscAI CASTG 2 cut(s) 40, 1156
TseFI GTSAC 2 cut(s) 474, 996
TseI GCWGC 2 cut(s) 72, 1096
Tsp45I GTSAC 2 cut(s) 474, 996
TspDTI ATGAA 2 cut(s) 390, 1037
TspRI CASTG 2 cut(s) 40, 1156
Van91I CCANNNNNTGG 1 cut(s) 740
VpaK11BI GGWCC 1 cut(s) 113
VspI ATTAAT 1 cut(s) 18
XapI RAATTY 4 cut(s) 138, 181, 196, 1024
XcmI CCANNNNNNNNNTGG 1 cut(s) 776
XspI CTAG 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.