MD03G1184200.v1.1

cysteine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014007
Physical Location & Seq
Forward (+)
25116470 .. 25119464
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1184200.v1.1.491

Sequence Viewer

Length: 1275 bp
ATGGCGCCTGTGAGAAGCACAGGCGCTATTGTGGCGGCAGTCTCTGTGTCAATGGCGTTGCTCTCTCTCTTCCTCTACAAGTCCAAACTCTCCTCCAAAAAGACCAAACTAACCCTGCCCTCGAAGAAAAATCACACGAATGGCCTCGTTGGTGCCATCGGCAACACCCCTCTGATCCGCATCAACAGCCTCTCTGACGCCACCGGCTGTGAAATTCTTGGGAAGTGCGAGTTTTTGAACCCGGGAGGGAGTGTGAAAGACAGAGTTGCTGTCAAAATTATTGCAGAGGCTTTGGAATCTGGAGACCTTGCTCCAGGTGGAGTAGTCACTGAGGGGAGTGCAGGAAGCACAGCTATAAGCCTTGCTACGGTTGCTCCTGCTTATGGTTGCAAATGTCACGTGGTTATACCTGATGACGCTGCTATTGAGAAGTCTCAAATACTTGAAGCCCTGGGAGCGACTGTTGAAAGGGTAAGACCAGTGTCAATTACGCACAGAGACCACTTTGTGAATATTGCTAGGCGACGGGCAACGGAAGCAAATGAATTAGCAATGAAGCATAGGAAAGATGAACAAATGAATGGCCAAGCCCTTGAGCAGACTAATGGTTTCCAATGTGATGGAGAGAAACAAAGTGTGGTATCAAGTTACACAGGTGGTTACTTTGCTGATCAGTTTGAAAATATGGCAAACTTCCGGGCCCACTATGAGGGTACCGGGCCTGAAATTTGGGAACAGACTGGAGGAAATGTAGATGCATTTGTGGCGGCAGCAGGAACAGGTGGTACAGTGGCTGGTGTGTCCAGGTTTCTCCAGGAAAAGAAGTCTAATGTTAAGTGCTTCCTGATAGATCCCCCTGGTTCCGGTTTATACAATAAAGTTACAAGGGGAGTGATGTACACTAGAGAGGAGGCTGAAGGACGAAGGCTAAAAAATCCATTTGACACGATAACTGAAGGAATTGGAATCAATAGAATAACAAAGAACTTTATGATGGCAAAACTTGACGGGGCTTTCCGAGGCACAGATAAAGAAGCTGTTGAAATGTCCAGGTTTCTTATGAAGAACGATGGGCTCTTTTTGGGGAGTTCTTCGGCCATGAATTGTGTTGGAGCAGTCCGAGCGGCACAGGCACTTGGTCCCGGTCACACAATTGTGACCATTTTATGCGATAGTGGGATGAGACATTTGAGCAAATTTTTCAGCGCTGAGTATCTGTCTCAATATGGATTGACACCCTCGGCTAGCGGATTAGAGTTCCTGGGTGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

425

Amino Acids

45.09

Weight (kDa)

8.51

Isoelectric Point (pI)

29.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 52 - 391 1.1e-65 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55880 AT1G55880
fragaria_vesca FvH4_3g29380 FvH4_3g29380
malus_domestica MD03G1184200.v1.1
prunus_persica Prupe.4G242200_v2.0.a1
pyrus_communis pycom03g13750
rosa_chinensis RchiOBHm_Chr5g0053771
rosa_laevigata RLG00000034902
rosa_multiflora Rmu_sc0001759.1_g000024
rosa_roxburghii Rroxscaffold_1G00026820
rosa_rugosa Rorug05G0282500 Rorug05G0282600 Rorug05G0282700
rosa_samantha Rh5BG364700 Rh5DG379500
rosa_wichuraiana Rw5G033350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 713
AccB1I GGYRCC 3 cut(s) 4, 152, 713
AccBSI CCGCTC 1 cut(s) 1124
AciI CCGC 5 cut(s) 35, 178, 767, 1124, 1248
AclWI GGATC 2 cut(s) 169, 845
AcoI YGGCCR 2 cut(s) 583, 1095
AcsI RAATTY 3 cut(s) 213, 726, 1196
AcuI CTGAAG 2 cut(s) 936, 975
AcvI CACGTG 1 cut(s) 400
AcyI GRCGYC 2 cut(s) 5, 198
AdeI CACNNNGTG 1 cut(s) 508
AfaI GTAC 3 cut(s) 715, 787, 899
AfeI AGCGCT 1 cut(s) 1207
AfiI CCNNNNNNNGG 4 cut(s) 367, 383, 709, 863
AgsI TTSAA 5 cut(s) 238, 446, 467, 680, 1043
AjnI CCWGG 7 cut(s) 313, 450, 803, 813, 856, 1049, 1260
AleI CACNNNNGTG 1 cut(s) 1154
AluBI AGCT 2 cut(s) 353, 1037
AluI AGCT 2 cut(s) 353, 1037
Alw26I GTCTC 6 cut(s) 46, 297, 438, 492, 1177, 1224
AlwI GGATC 2 cut(s) 169, 845
AlwNI CAGNNNCTG 2 cut(s) 44, 794
Ama87I CYCGRG 1 cut(s) 241
Aor51HI AGCGCT 1 cut(s) 1207
AoxI GGCC 5 cut(s) 142, 583, 699, 719, 1095
ApaI GGGCCC 1 cut(s) 703
ApeKI GCWGC 2 cut(s) 419, 770
ApoI RAATTY 3 cut(s) 213, 726, 1196
Asp718I GGTACC 1 cut(s) 713
AspLEI GCGC 3 cut(s) 7, 26, 1208
AspS9I GGNCC 4 cut(s) 699, 700, 719, 1139
AsuC2I CCSGG 5 cut(s) 242, 243, 698, 718, 1143
AsuNHI GCTAGC 1 cut(s) 1244
AvaI CYCGRG 1 cut(s) 241
AvaII GGWCC 1 cut(s) 1139
BaeGI GKGCMC 1 cut(s) 703
BalI TGGCCA 1 cut(s) 585
BanI GGYRCC 3 cut(s) 4, 152, 713
BanII GRGCYC 2 cut(s) 703, 1077
BbrPI CACGTG 1 cut(s) 400
BbvI GCAGC 2 cut(s) 406, 782
BccI CCATC 4 cut(s) 164, 614, 988, 1064
BciT130I CCWGG 7 cut(s) 315, 452, 805, 815, 858, 1051, 1262
BclI TGATCA 1 cut(s) 670
BcnI CCSGG 5 cut(s) 242, 243, 698, 718, 1143
BcoDI GTCTC 6 cut(s) 46, 297, 438, 492, 1177, 1224
BfaI CTAG 3 cut(s) 519, 903, 1245
BfoI RGCGCY 3 cut(s) 8, 27, 1209
BisI GCNGC 5 cut(s) 36, 420, 768, 771, 1125
BlsI GCNGC 5 cut(s) 37, 421, 769, 772, 1126
Bme18I GGWCC 1 cut(s) 1139
BmeT110I CYCGRG 1 cut(s) 241
BmgT120I GGNCC 4 cut(s) 699, 700, 719, 1139
BmiI GGNNCC 6 cut(s) 6, 154, 701, 715, 862, 1141
BmsI GCATC 2 cut(s) 189, 745
BmtI GCTAGC 1 cut(s) 1248
BoxI GACNNNNGTC 1 cut(s) 481
BpmI CTGGAG 4 cut(s) 297, 321, 762, 797
BpuEI CTTGAG 1 cut(s) 614
BpuMI CCSGG 5 cut(s) 242, 243, 698, 718, 1143
BsaAI YACGTR 1 cut(s) 400
BsaBI GATNNNNATC 1 cut(s) 179
BsaHI GRCGYC 2 cut(s) 5, 198
BsaI GGTCTC 2 cut(s) 297, 492
BsaJI CCNNGG 7 cut(s) 241, 450, 451, 856, 1018, 1239, 1261
BsaWI WCCGGW 1 cut(s) 863
BsaXI ACNNNNNCTCC 8 cut(s) 237, 267, 358, 388, 447, 477, 735, 765
Bsc4I CCNNNNNNNGG 4 cut(s) 367, 383, 709, 863
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 479, 745
Bse3DI GCAATG 1 cut(s) 558
Bse8I GATNNNNATC 1 cut(s) 179
BseBI CCWGG 7 cut(s) 315, 452, 805, 815, 858, 1051, 1262
BseDI CCNNGG 7 cut(s) 241, 450, 451, 856, 1018, 1239, 1261
BseGI GGATG 1 cut(s) 1185
BseJI GATNNNNATC 1 cut(s) 179
BseLI CCNNNNNNNGG 4 cut(s) 367, 383, 709, 863
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 2 cut(s) 321, 1200
BseNI ACTGG 2 cut(s) 479, 745
BseRI GAGGAG 2 cut(s) 82, 923
BseSI GKGCMC 1 cut(s) 703
BseXI GCAGC 2 cut(s) 406, 782
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 5 cut(s) 144, 585, 701, 721, 1097
BshNI GGYRCC 3 cut(s) 4, 152, 713
BsiHKCI CYCGRG 1 cut(s) 241
BsiSI CCGG 6 cut(s) 204, 242, 697, 717, 864, 1143
BslFI GGGAC 1 cut(s) 1125
BslI CCNNNNNNNGG 4 cut(s) 367, 383, 709, 863
BsmAI GTCTC 6 cut(s) 46, 297, 438, 492, 1177, 1224
BsmFI GGGAC 1 cut(s) 1125
BsnI GGCC 5 cut(s) 144, 585, 701, 721, 1097
Bso31I GGTCTC 2 cut(s) 297, 492
BsoBI CYCGRG 1 cut(s) 241
Bsp120I GGGCCC 1 cut(s) 699
Bsp1286I GDGCHC 2 cut(s) 703, 1077
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 3 cut(s) 174, 670, 850
BspACI CCGC 5 cut(s) 35, 178, 767, 1124, 1248
BspANI GGCC 5 cut(s) 144, 585, 701, 721, 1097
BspCNI CTCAG 2 cut(s) 322, 1201
BspLI GGNNCC 6 cut(s) 6, 154, 701, 715, 862, 1141
BspOI GCTAGC 1 cut(s) 1248
BspPI GGATC 2 cut(s) 169, 845
BspT107I GGYRCC 3 cut(s) 4, 152, 713
BspTNI GGTCTC 2 cut(s) 297, 492
BsrBI CCGCTC 1 cut(s) 1124
BsrDI GCAATG 1 cut(s) 558
BsrFI RCCGGY 1 cut(s) 203
BsrGI TGTACA 1 cut(s) 897
BsrI ACTGG 2 cut(s) 479, 745
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 7 cut(s) 241, 450, 451, 856, 1018, 1239, 1261
BssMI GATC 3 cut(s) 174, 670, 850
BssNI GRCGYC 2 cut(s) 5, 198
Bst2UI CCWGG 7 cut(s) 315, 452, 805, 815, 858, 1051, 1262
Bst4CI ACNGT 3 cut(s) 370, 463, 790
Bst6I CTCTTC 1 cut(s) 74
BstACI GRCGYC 2 cut(s) 5, 198
BstAUI TGTACA 1 cut(s) 897
BstBAI YACGTR 1 cut(s) 400
BstC8I GCNNGC 1 cut(s) 1246
BstDEI CTNAG 2 cut(s) 330, 1209
BstF5I GGATG 1 cut(s) 1185
BstH2I RGCGCY 3 cut(s) 8, 27, 1209
BstHHI GCGC 3 cut(s) 7, 26, 1208
BstKTI GATC 3 cut(s) 177, 673, 853
BstMAI GTCTC 6 cut(s) 46, 297, 438, 492, 1177, 1224
BstMBI GATC 3 cut(s) 174, 670, 850
BstMWI GCNNNNNNNGC 8 cut(s) 32, 186, 371, 455, 536, 764, 1121, 1130
BstNI CCWGG 7 cut(s) 315, 452, 805, 815, 858, 1051, 1262
BstPAI GACNNNNGTC 1 cut(s) 481
BstSLI GKGCMC 1 cut(s) 703
BstV1I GCAGC 2 cut(s) 406, 782
BstX2I RGATCY 1 cut(s) 850
BstXI CCANNNNNNTGG 1 cut(s) 620
BstYI RGATCY 1 cut(s) 850
BsuRI GGCC 5 cut(s) 144, 585, 701, 721, 1097
BtsCI GGATG 1 cut(s) 1185
BtsIMutI CAGTG 3 cut(s) 327, 486, 795
Cac8I GCNNGC 1 cut(s) 1246
CaiI CAGNNNCTG 2 cut(s) 44, 794
CfoI GCGC 3 cut(s) 7, 26, 1208
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 4 cut(s) 699, 700, 719, 1139
Cfr9I CCCGGG 1 cut(s) 241
CseI GACGC 2 cut(s) 206, 425
Csp6I GTAC 3 cut(s) 714, 786, 898
CviAII CATG 1 cut(s) 1099
CviQI GTAC 3 cut(s) 714, 786, 898
DdeI CTNAG 2 cut(s) 330, 1209
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 176, 672, 852
DpnII GATC 3 cut(s) 174, 670, 850
DraIII CACNNNGTG 1 cut(s) 508
EaeI YGGCCR 2 cut(s) 583, 1095
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco24I GRGCYC 2 cut(s) 703, 1077
Eco31I GGTCTC 2 cut(s) 297, 492
Eco47I GGWCC 1 cut(s) 1139
Eco47III AGCGCT 1 cut(s) 1207
Eco57I CTGAAG 2 cut(s) 936, 975
Eco72I CACGTG 1 cut(s) 400
Eco88I CYCGRG 1 cut(s) 241
EcoRII CCWGG 7 cut(s) 313, 450, 803, 813, 856, 1049, 1260
EcoT22I ATGCAT 1 cut(s) 760
EcoT38I GRGCYC 2 cut(s) 703, 1077
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
FaeI CATG 1 cut(s) 1102
FaqI GGGAC 1 cut(s) 1125
FatI CATG 1 cut(s) 1098
FbaI TGATCA 1 cut(s) 670
Fnu4HI GCNGC 5 cut(s) 36, 420, 768, 771, 1125
FokI GGATG 1 cut(s) 1192
FriOI GRGCYC 2 cut(s) 703, 1077
Fsp4HI GCNGC 5 cut(s) 36, 420, 768, 771, 1125
FspBI CTAG 3 cut(s) 519, 903, 1245
GlaI GCGC 3 cut(s) 6, 25, 1207
GluI GCNGC 5 cut(s) 36, 420, 768, 771, 1125
GsuI CTGGAG 4 cut(s) 297, 321, 762, 797
HaeII RGCGCY 3 cut(s) 8, 27, 1209
HaeIII GGCC 5 cut(s) 144, 585, 701, 721, 1097
HapII CCGG 6 cut(s) 204, 242, 697, 717, 864, 1143
HgaI GACGC 2 cut(s) 206, 425
HhaI GCGC 3 cut(s) 7, 26, 1208
Hin1I GRCGYC 2 cut(s) 5, 198
Hin1II CATG 1 cut(s) 1102
Hin6I GCGC 3 cut(s) 5, 24, 1206
HinP1I GCGC 3 cut(s) 5, 24, 1206
HinfI GANTC 2 cut(s) 296, 966
HpaII CCGG 6 cut(s) 204, 242, 697, 717, 864, 1143
Hpy166II GTNNAC 1 cut(s) 900
Hpy188I TCNGA 4 cut(s) 174, 196, 1019, 1121
Hpy188III TCNNGA 2 cut(s) 300, 844
Hpy8I GTNNAC 1 cut(s) 900
Hpy99I CGWCG 1 cut(s) 528
HpyAV CCTTC 3 cut(s) 911, 918, 950
HpyCH4III ACNGT 3 cut(s) 370, 463, 790
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 4 cut(s) 284, 341, 390, 758
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 186, 371, 455, 536, 764, 1121, 1130
HpyF3I CTNAG 2 cut(s) 330, 1209
HpySE526I ACGT 1 cut(s) 399
Hsp92I GRCGYC 2 cut(s) 5, 198
Hsp92II CATG 1 cut(s) 1102
HspAI GCGC 3 cut(s) 5, 24, 1206
KasI GGCGCC 1 cut(s) 4
KpnI GGTACC 1 cut(s) 717
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 3 cut(s) 174, 670, 850
LmnI GCTCC 4 cut(s) 316, 379, 455, 1112
Lsp1109I GCAGC 2 cut(s) 406, 782
LweI GCATC 2 cut(s) 189, 745
MaeI CTAG 3 cut(s) 519, 903, 1245
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 7 cut(s) 325, 395, 647, 659, 880, 1145, 1156
MalI GATC 3 cut(s) 176, 672, 852
MbiI CCGCTC 1 cut(s) 1124
MboI GATC 3 cut(s) 174, 670, 850
MboII GAAGA 4 cut(s) 61, 136, 1075, 1083
MfeI CAATTG 1 cut(s) 1152
MflI RGATCY 1 cut(s) 850
MhlI GDGCHC 2 cut(s) 703, 1077
MlsI TGGCCA 1 cut(s) 585
MluCI AATT 9 cut(s) 213, 276, 486, 545, 726, 960, 1102, 1152, 1196
MluNI TGGCCA 1 cut(s) 585
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 1090, 1249
Mox20I TGGCCA 1 cut(s) 585
Mph1103I ATGCAT 1 cut(s) 760
MscI TGGCCA 1 cut(s) 585
MseI TTAA 1 cut(s) 834
MslI CAYNNNNRTG 2 cut(s) 138, 1154
Msp20I TGGCCA 1 cut(s) 585
MspI CCGG 6 cut(s) 204, 242, 697, 717, 864, 1143
MunI CAATTG 1 cut(s) 1152
MvaI CCWGG 7 cut(s) 315, 452, 805, 815, 858, 1051, 1262
MwoI GCNNNNNNNGC 8 cut(s) 32, 186, 371, 455, 536, 764, 1121, 1130
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 5 cut(s) 242, 243, 698, 718, 1143
NdeII GATC 3 cut(s) 174, 670, 850
NheI GCTAGC 1 cut(s) 1244
NlaIII CATG 1 cut(s) 1102
NlaIV GGNNCC 6 cut(s) 6, 154, 701, 715, 862, 1141
NmeAIII GCCGAG 1 cut(s) 1220
NmuCI GTSAC 4 cut(s) 325, 395, 1145, 1156
NsiI ATGCAT 1 cut(s) 760
OliI CACNNNNGTG 1 cut(s) 1154
PasI CCCWGGG 1 cut(s) 451
PfeI GAWTC 2 cut(s) 296, 966
PfoI TCCNGGA 1 cut(s) 813
PkrI GCNGC 5 cut(s) 37, 421, 769, 772, 1126
PluTI GGCGCC 1 cut(s) 8
PmaCI CACGTG 1 cut(s) 400
PmlI CACGTG 1 cut(s) 400
Ppu21I YACGTR 1 cut(s) 400
PshAI GACNNNNGTC 1 cut(s) 481
Psp6I CCWGG 7 cut(s) 313, 450, 803, 813, 856, 1049, 1260
PspCI CACGTG 1 cut(s) 400
PspGI CCWGG 7 cut(s) 313, 450, 803, 813, 856, 1049, 1260
PspN4I GGNNCC 6 cut(s) 6, 154, 701, 715, 862, 1141
PspOMI GGGCCC 1 cut(s) 699
PspPI GGNCC 4 cut(s) 699, 700, 719, 1139
PsrI GAACNNNNNNTAC 2 cut(s) 769, 801
PstNI CAGNNNCTG 2 cut(s) 44, 794
PsuI RGATCY 1 cut(s) 850
RsaI GTAC 3 cut(s) 715, 787, 899
RsaNI GTAC 3 cut(s) 714, 786, 898
RseI CAYNNNNRTG 2 cut(s) 138, 1154
SaqAI TTAA 1 cut(s) 834
SatI GCNGC 5 cut(s) 36, 420, 768, 771, 1125
Sau3AI GATC 3 cut(s) 174, 670, 850
Sau96I GGNCC 4 cut(s) 699, 700, 719, 1139
SduI GDGCHC 2 cut(s) 703, 1077
SfaNI GCATC 2 cut(s) 189, 745
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 1139
SmaI CCCGGG 1 cut(s) 243
SmiMI CAYNNNNRTG 2 cut(s) 138, 1154
SmlI CTYRAG 1 cut(s) 593
SmoI CTYRAG 1 cut(s) 593
Sse9I AATT 9 cut(s) 213, 276, 486, 545, 726, 960, 1102, 1152, 1196
SsiI CCGC 5 cut(s) 35, 178, 767, 1124, 1248
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 514
SspMI CTAG 3 cut(s) 519, 903, 1245
TaaI ACNGT 3 cut(s) 370, 463, 790
TaiI ACGT 1 cut(s) 402
TaqI TCGA 1 cut(s) 122
TasI AATT 9 cut(s) 213, 276, 486, 545, 726, 960, 1102, 1152, 1196
TatI WGTACW 1 cut(s) 897
TauI GCSGC 3 cut(s) 38, 770, 1127
TfiI GAWTC 2 cut(s) 296, 966
Tru1I TTAA 1 cut(s) 834
Tru9I TTAA 1 cut(s) 834
TscAI CASTG 3 cut(s) 334, 486, 795
TseFI GTSAC 4 cut(s) 325, 395, 1145, 1156
TseI GCWGC 2 cut(s) 419, 770
Tsp45I GTSAC 4 cut(s) 325, 395, 1145, 1156
TspDTI ATGAA 6 cut(s) 558, 569, 585, 593, 1076, 1115
TspGWI ACGGA 1 cut(s) 548
TspMI CCCGGG 1 cut(s) 241
TspRI CASTG 3 cut(s) 334, 486, 795
VpaK11BI GGWCC 1 cut(s) 1139
XapI RAATTY 3 cut(s) 213, 726, 1196
XmaI CCCGGG 1 cut(s) 241
XspI CTAG 3 cut(s) 519, 903, 1245
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.