RchiOBHm_Chr5g0053771

cysteine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0014007
Physical Location & Seq
Reverse (-)
56111240 .. 56114863
3624 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33103

Sequence Viewer

Length: 1263 bp
ATGGCGCCTGTGAGAATTACAGGCGCTATGGTCGCTGCAATCTCAGTGACCATGGCCTTGCTCTCTATAGTCCTCTACAAGTCCAAGAAGACCAAACCGGCCTTACCCTCAAACAAAAATCCCAGAAATGGTCTCGTTGGAGCCATTGGCAACACCCCTCTCATTCGCATCAACAGCCTCTCTGACGCCACTGGTTGTGAAATTCTTGGGAAGTGCGAGTTTTTGAACCCAGGAGGGAGTGTGAAAGATAGAGTTGCTGTGAAAATTATTGAAGAGGCTTTGGAATCTGGAGACCTAGCTCCAGGTGGAGTAGTCACTGAGGGAAGTGCTGGAAGCACAGCTATTAGCCTTGCTACAGTTGCTCCTGCTTATGGTTGCAAATGCCATGTGGTTATACCTGATGACGCTGCTATTGAGAAGTCTCAGATACTTGAAGCCCTGGGAGCCACTGTTGAGAGGGTAAGGCCAGTTTCAATCACACACAGGGATCACTTTGTCAACATTGCTAGGCGACGGGCATCAGAAGCAAATGAGTTAGCCTTAAAGCATAGAAAAGATGAGCAAAAGAATGGCAAAACCCTTGAGCAAGTTAATGGTTTCCATTATGATGAAGAGAAACAGTTGTTTCCAAGGAATTGTCCTGGTGGATACTTTGCTGATCAATTTGAAAACTTGGCAAACTTCCGGGCACACTATGAGGGTACTGGGCCTGAGATTTGGGAGCAGACTGGAGGAAATGTAGATGCATTTGTGGCGGCAGCAGGAACAGGTGGTACTGTTGCTGGTGCTTCCAAGTTTCTACAGGAAAGGAATCCAAACATCAAATGCTTCCTGATAGACCCTCCTGGTTCTGGTTTGTTTAATAAGATTACGAGGGGAGTGATGTACACCAAAGAGGAGGCTGAAGGGCGAAGGCTAAAGAATCCATTTGACACGATAACTGAAGGAATTGGAATCAATAGACTAACAAAGAATTTTCTGATGGCAAAGCTTGATGGGGCTTTCCGAGGCACAGATAAGGAAGCTGTTGAAATGTCCAGGTTTCTGATGAAGAACGATGGGCTCTTCATGGGGAGTTCTTCAGCTATGAATTGTGTTGGAGCAGTTCGAGTGGCACAGTCAATTGGTCCTGGTCACACTATTGTCACCATATTGTGTGATAGTGGGATGAGACATCTGAGCAAATTTTACAATGCTGAGTATCTGTCTAAATTTGATTTGACACCCACTGCGTGCGGATTAGAGTTCCTGGGTGTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

420

Amino Acids

45.04

Weight (kDa)

8.28

Isoelectric Point (pI)

36.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PALP PF00291 48 - 387 2.8e-65 Pyridoxal-phosphate dependent enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014007)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55880 AT1G55880
fragaria_vesca FvH4_3g29380 FvH4_3g29380
malus_domestica MD03G1184200.v1.1
prunus_persica Prupe.4G242200_v2.0.a1
pyrus_communis pycom03g13750
rosa_chinensis RchiOBHm_Chr5g0053771
rosa_laevigata RLG00000034902
rosa_multiflora Rmu_sc0001759.1_g000024
rosa_roxburghii Rroxscaffold_1G00026820
rosa_rugosa Rorug05G0282500 Rorug05G0282600 Rorug05G0282700
rosa_samantha Rh5BG364700 Rh5DG379500
rosa_wichuraiana Rw5G033350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 2 cut(s) 755, 1236
AclWI GGATC 1 cut(s) 495
AcsI RAATTY 4 cut(s) 201, 973, 1184, 1211
AcuI CTGAAG 3 cut(s) 924, 963, 1065
AcyI GRCGYC 2 cut(s) 5, 186
AdeI CACNNNGTG 1 cut(s) 1233
AfaI GTAC 3 cut(s) 703, 775, 887
AfiI CCNNNNNNNGG 3 cut(s) 128, 371, 851
AgsI TTSAA 6 cut(s) 226, 272, 434, 474, 668, 1031
AjnI CCWGG 8 cut(s) 229, 301, 438, 640, 844, 1037, 1129, 1248
AluBI AGCT 5 cut(s) 299, 341, 991, 1025, 1085
AluI AGCT 5 cut(s) 299, 341, 991, 1025, 1085
Alw26I GTCTC 4 cut(s) 137, 285, 426, 1165
AlwI GGATC 1 cut(s) 495
AoxI GGCC 4 cut(s) 54, 99, 464, 707
ApeKI GCWGC 3 cut(s) 35, 407, 758
ApoI RAATTY 4 cut(s) 201, 973, 1184, 1211
AspLEI GCGC 2 cut(s) 7, 26
AspS9I GGNCC 2 cut(s) 707, 1127
AsuC2I CCSGG 1 cut(s) 686
AsuHPI GGTGA 1 cut(s) 1138
AvaII GGWCC 1 cut(s) 1127
BaeGI GKGCMC 1 cut(s) 691
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 1065
BbsI GAAGAC 1 cut(s) 95
BbvI GCAGC 3 cut(s) 22, 394, 770
BccI CCATC 3 cut(s) 976, 989, 1052
BciT130I CCWGG 8 cut(s) 231, 303, 440, 642, 846, 1039, 1131, 1250
BciVI GTATCC 1 cut(s) 641
BclI TGATCA 1 cut(s) 658
BcnI CCSGG 1 cut(s) 686
BcoDI GTCTC 4 cut(s) 137, 285, 426, 1165
BfaI CTAG 2 cut(s) 296, 507
BfmI CTRYAG 3 cut(s) 66, 354, 800
BfoI RGCGCY 2 cut(s) 8, 27
BfuI GTATCC 1 cut(s) 641
BisI GCNGC 4 cut(s) 36, 408, 756, 759
BlsI GCNGC 4 cut(s) 37, 409, 757, 760
Bme1390I CCNGG 9 cut(s) 231, 303, 440, 642, 686, 846, 1039, 1131, 1250
Bme18I GGWCC 1 cut(s) 1127
BmgT120I GGNCC 2 cut(s) 707, 1127
BmiI GGNNCC 3 cut(s) 6, 142, 445
BmrFI CCNGG 9 cut(s) 231, 303, 440, 642, 686, 846, 1039, 1131, 1250
BmrI ACTGGG 1 cut(s) 714
BmsI GCATC 3 cut(s) 177, 527, 733
BmuI ACTGGG 1 cut(s) 714
BpiI GAAGAC 1 cut(s) 95
BpmI CTGGAG 3 cut(s) 285, 309, 750
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 1 cut(s) 686
BsaHI GRCGYC 2 cut(s) 5, 186
BsaI GGTCTC 2 cut(s) 137, 285
BsaJI CCNNGG 7 cut(s) 51, 229, 438, 439, 629, 1006, 1249
BsaXI ACNNNNNCTCC 8 cut(s) 225, 255, 346, 376, 435, 465, 723, 753
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 371, 851
Bse118I RCCGGY 1 cut(s) 97
Bse1I ACTGG 4 cut(s) 196, 467, 709, 733
Bse3DI GCAATG 1 cut(s) 501
BseBI CCWGG 8 cut(s) 231, 303, 440, 642, 846, 1039, 1131, 1250
BseDI CCNNGG 7 cut(s) 51, 229, 438, 439, 629, 1006, 1249
BseGI GGATG 1 cut(s) 1173
BseLI CCNNNNNNNGG 3 cut(s) 128, 371, 851
BseMI GCAATG 1 cut(s) 501
BseMII CTCAG 6 cut(s) 57, 309, 437, 702, 1169, 1188
BseNI ACTGG 4 cut(s) 196, 467, 709, 733
BseRI GAGGAG 1 cut(s) 911
BseSI GKGCMC 1 cut(s) 691
BseXI GCAGC 3 cut(s) 22, 394, 770
BshFI GGCC 4 cut(s) 56, 101, 466, 709
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 2 cut(s) 98, 685
BslI CCNNNNNNNGG 3 cut(s) 128, 371, 851
BsmAI GTCTC 4 cut(s) 137, 285, 426, 1165
BsnI GGCC 4 cut(s) 56, 101, 466, 709
Bso31I GGTCTC 2 cut(s) 137, 285
Bsp1286I GDGCHC 2 cut(s) 691, 1065
Bsp1407I TGTACA 1 cut(s) 885
Bsp143I GATC 2 cut(s) 487, 658
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 2 cut(s) 755, 1236
BspANI GGCC 4 cut(s) 56, 101, 466, 709
BspCNI CTCAG 6 cut(s) 56, 310, 436, 703, 1170, 1189
BspLI GGNNCC 3 cut(s) 6, 142, 445
BspPI GGATC 1 cut(s) 495
BspQI GCTCTTC 1 cut(s) 1070
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 2 cut(s) 137, 285
BsrDI GCAATG 1 cut(s) 501
BsrFI RCCGGY 1 cut(s) 97
BsrGI TGTACA 1 cut(s) 885
BsrI ACTGG 4 cut(s) 196, 467, 709, 733
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 7 cut(s) 51, 229, 438, 439, 629, 1006, 1249
BssMI GATC 2 cut(s) 487, 658
BssNI GRCGYC 2 cut(s) 5, 186
BssT1I CCWWGG 2 cut(s) 51, 629
Bst2UI CCWGG 8 cut(s) 231, 303, 440, 642, 846, 1039, 1131, 1250
Bst4CI ACNGT 5 cut(s) 358, 451, 621, 778, 1119
Bst6I CTCTTC 3 cut(s) 267, 606, 1070
BstACI GRCGYC 2 cut(s) 5, 186
BstAUI TGTACA 1 cut(s) 885
BstC8I GCNNGC 1 cut(s) 1234
BstDEI CTNAG 6 cut(s) 43, 318, 423, 711, 1178, 1197
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 1 cut(s) 1173
BstH2I RGCGCY 2 cut(s) 8, 27
BstHHI GCGC 2 cut(s) 7, 26
BstKTI GATC 2 cut(s) 490, 661
BstMAI GTCTC 4 cut(s) 137, 285, 426, 1165
BstMBI GATC 2 cut(s) 487, 658
BstMWI GCNNNNNNNGC 6 cut(s) 32, 174, 359, 443, 524, 752
BstNI CCWGG 8 cut(s) 231, 303, 440, 642, 846, 1039, 1131, 1250
BstSCI CCNGG 9 cut(s) 229, 301, 438, 640, 684, 844, 1037, 1129, 1248
BstSFI CTRYAG 3 cut(s) 66, 354, 800
BstSLI GKGCMC 1 cut(s) 691
BstV1I GCAGC 3 cut(s) 22, 394, 770
BstV2I GAAGAC 1 cut(s) 95
BsuI GTATCC 1 cut(s) 641
BsuRI GGCC 4 cut(s) 56, 101, 466, 709
BtgI CCRYGG 1 cut(s) 51
BtsCI GGATG 1 cut(s) 1173
BtsI GCAGTG 1 cut(s) 1227
BtsIMutI CAGTG 5 cut(s) 51, 189, 315, 447, 1227
Cac8I GCNNGC 1 cut(s) 1234
CfoI GCGC 2 cut(s) 7, 26
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 2 cut(s) 707, 1127
CseI GACGC 2 cut(s) 194, 413
Csp6I GTAC 3 cut(s) 702, 774, 886
CviAII CATG 3 cut(s) 52, 386, 1069
CviQI GTAC 3 cut(s) 702, 774, 886
DdeI CTNAG 6 cut(s) 43, 318, 423, 711, 1178, 1197
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 489, 660
DpnII GATC 2 cut(s) 487, 658
DraIII CACNNNGTG 1 cut(s) 1233
Eam1104I CTCTTC 3 cut(s) 267, 606, 1070
EarI CTCTTC 3 cut(s) 267, 606, 1070
Eco130I CCWWGG 2 cut(s) 51, 629
Eco24I GRGCYC 1 cut(s) 1065
Eco31I GGTCTC 2 cut(s) 137, 285
Eco47I GGWCC 1 cut(s) 1127
Eco57I CTGAAG 3 cut(s) 924, 963, 1065
EcoRII CCWGG 8 cut(s) 229, 301, 438, 640, 844, 1037, 1129, 1248
EcoT14I CCWWGG 2 cut(s) 51, 629
EcoT22I ATGCAT 1 cut(s) 748
EcoT38I GRGCYC 1 cut(s) 1065
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 51, 629
FaeI CATG 3 cut(s) 55, 389, 1072
FatI CATG 3 cut(s) 51, 385, 1068
FbaI TGATCA 1 cut(s) 658
Fnu4HI GCNGC 4 cut(s) 36, 408, 756, 759
FokI GGATG 1 cut(s) 1180
FriOI GRGCYC 1 cut(s) 1065
Fsp4HI GCNGC 4 cut(s) 36, 408, 756, 759
FspBI CTAG 2 cut(s) 296, 507
GlaI GCGC 2 cut(s) 6, 25
GluI GCNGC 4 cut(s) 36, 408, 756, 759
GsuI CTGGAG 3 cut(s) 285, 309, 750
HaeII RGCGCY 2 cut(s) 8, 27
HaeIII GGCC 4 cut(s) 56, 101, 466, 709
HapII CCGG 2 cut(s) 98, 685
HgaI GACGC 2 cut(s) 194, 413
HhaI GCGC 2 cut(s) 7, 26
Hin1I GRCGYC 2 cut(s) 5, 186
Hin1II CATG 3 cut(s) 55, 389, 1072
Hin6I GCGC 2 cut(s) 5, 24
HinP1I GCGC 2 cut(s) 5, 24
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HindIII AAGCTT 1 cut(s) 989
HinfI GANTC 4 cut(s) 284, 811, 922, 954
HpaII CCGG 2 cut(s) 98, 685
HphI GGTGA 1 cut(s) 1138
Hpy166II GTNNAC 2 cut(s) 499, 888
Hpy188I TCNGA 7 cut(s) 184, 426, 523, 981, 1007, 1047, 1179
Hpy188III TCNNGA 2 cut(s) 288, 832
Hpy8I GTNNAC 2 cut(s) 499, 888
Hpy99I CGWCG 1 cut(s) 516
HpyAV CCTTC 3 cut(s) 899, 906, 938
HpyCH4III ACNGT 5 cut(s) 358, 451, 621, 778, 1119
HpyCH4V TGCA 3 cut(s) 38, 378, 746
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 174, 359, 443, 524, 752
HpyF3I CTNAG 6 cut(s) 43, 318, 423, 711, 1178, 1197
Hsp92I GRCGYC 2 cut(s) 5, 186
Hsp92II CATG 3 cut(s) 55, 389, 1072
HspAI GCGC 2 cut(s) 5, 24
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 658
Kzo9I GATC 2 cut(s) 487, 658
LguI GCTCTTC 1 cut(s) 1070
LmnI GCTCC 6 cut(s) 140, 304, 367, 443, 721, 1100
Lsp1109I GCAGC 3 cut(s) 22, 394, 770
LweI GCATC 3 cut(s) 177, 527, 733
MaeI CTAG 2 cut(s) 296, 507
MaeIII GTNAC 4 cut(s) 46, 313, 1133, 1144
MalI GATC 2 cut(s) 489, 660
MboI GATC 2 cut(s) 487, 658
MboII GAAGA 6 cut(s) 100, 284, 623, 1057, 1063, 1071
MfeI CAATTG 1 cut(s) 1122
MhlI GDGCHC 2 cut(s) 691, 1065
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 118, 1078, 1237
Mph1103I ATGCAT 1 cut(s) 748
MseI TTAA 3 cut(s) 542, 591, 861
MslI CAYNNNNRTG 1 cut(s) 606
MspI CCGG 2 cut(s) 98, 685
MspR9I CCNGG 9 cut(s) 231, 303, 440, 642, 686, 846, 1039, 1131, 1250
MunI CAATTG 1 cut(s) 1122
MvaI CCWGG 8 cut(s) 231, 303, 440, 642, 846, 1039, 1131, 1250
MwoI GCNNNNNNNGC 6 cut(s) 32, 174, 359, 443, 524, 752
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 686
NcoI CCATGG 1 cut(s) 51
NdeII GATC 2 cut(s) 487, 658
NlaIII CATG 3 cut(s) 55, 389, 1072
NlaIV GGNNCC 3 cut(s) 6, 142, 445
NmuCI GTSAC 4 cut(s) 46, 313, 1133, 1144
NsiI ATGCAT 1 cut(s) 748
PasI CCCWGGG 1 cut(s) 439
PciSI GCTCTTC 1 cut(s) 1070
PfeI GAWTC 4 cut(s) 284, 811, 922, 954
PkrI GCNGC 4 cut(s) 37, 409, 757, 760
PluTI GGCGCC 1 cut(s) 8
Psp6I CCWGG 8 cut(s) 229, 301, 438, 640, 844, 1037, 1129, 1248
PspGI CCWGG 8 cut(s) 229, 301, 438, 640, 844, 1037, 1129, 1248
PspN4I GGNNCC 3 cut(s) 6, 142, 445
PspPI GGNCC 2 cut(s) 707, 1127
PsrI GAACNNNNNNTAC 2 cut(s) 757, 789
RsaI GTAC 3 cut(s) 703, 775, 887
RsaNI GTAC 3 cut(s) 702, 774, 886
RseI CAYNNNNRTG 1 cut(s) 606
SapI GCTCTTC 1 cut(s) 1070
SaqAI TTAA 3 cut(s) 542, 591, 861
SatI GCNGC 4 cut(s) 36, 408, 756, 759
Sau3AI GATC 2 cut(s) 487, 658
Sau96I GGNCC 2 cut(s) 707, 1127
ScrFI CCNGG 9 cut(s) 231, 303, 440, 642, 686, 846, 1039, 1131, 1250
SduI GDGCHC 2 cut(s) 691, 1065
SfaNI GCATC 3 cut(s) 177, 527, 733
SfcI CTRYAG 3 cut(s) 66, 354, 800
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 1 cut(s) 1127
SmiMI CAYNNNNRTG 1 cut(s) 606
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
SsiI CCGC 2 cut(s) 755, 1236
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 2 cut(s) 296, 507
StyD4I CCNGG 9 cut(s) 229, 301, 438, 640, 684, 844, 1037, 1129, 1248
StyI CCWWGG 2 cut(s) 51, 629
TaaI ACNGT 5 cut(s) 358, 451, 621, 778, 1119
TaqI TCGA 1 cut(s) 1108
TatI WGTACW 1 cut(s) 885
TauI GCSGC 1 cut(s) 758
TfiI GAWTC 4 cut(s) 284, 811, 922, 954
Tru1I TTAA 3 cut(s) 542, 591, 861
Tru9I TTAA 3 cut(s) 542, 591, 861
TscAI CASTG 5 cut(s) 51, 196, 322, 454, 1234
TseFI GTSAC 4 cut(s) 46, 313, 1133, 1144
TseI GCWGC 3 cut(s) 35, 407, 758
Tsp45I GTSAC 4 cut(s) 46, 313, 1133, 1144
TspDTI ATGAA 4 cut(s) 624, 1057, 1064, 1103
TspRI CASTG 5 cut(s) 51, 196, 322, 454, 1234
VpaK11BI GGWCC 1 cut(s) 1127
XapI RAATTY 4 cut(s) 201, 973, 1184, 1211
XspI CTAG 2 cut(s) 296, 507
Zsp2I ATGCAT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.