FvH4_3g34690

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012947
Physical Location & Seq
Reverse (-)
30029399 .. 30032339
2941 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g34690.t1

Sequence Viewer

Length: 1398 bp
ATGGAGTTTCTGAGTATACTCTATGCTCTCTCAGCTTTGTATTTCATTTTCACTTTGTGGAAGCGTTATGATGAAAAAAGAGACCAACAATGTTACATCTTGGACTACGAATGCCACAAGCCCACAGATGACAGGAAGCTTGACACAGAGTATTCAGGGGAGATCATCAAGAGGACCAAGAATTTGGGTCTTCTAGAGTACCAGTTTCTCCTCAAAGCCATAGTGAGCTCCGGCATTGGCGAGCAAACCTATGCGCCGAGGATCATGTTCGACTGTAGGGAATCATGTCCTACATTGGAAGATTCAATCACAGAGATGGAGGAGTTCTTCCACGACAGCATTCAGAAGGTGTTGGACAAACAAGGCCTTTCCCCTTCCCAGATTGATGTGCTTGTGGTCAATGTGTCAATGTTTGCTTCTGTGCCTTCTTTAGCCTCGAGGATCATAAACCACTACAAGATGAGAGAGGACATAAAGGTCTATAACCTCAGCGGGATGGGTTGCTCAGCTAGTCTGATCTCAGTTGGCATAGTCAGGAGCATTTTCAAAACAAACAAGAACATTTATGCACTTGTGGTTACTTCAGAGTCCCTGAGCCCCAACTGGTACACAGGTAATGCCAGATCCATGATTCTTGCAAATTGTTTGTTTAGGTCTGGTGGATGTGCTATCCTTTTGACTAACAAAAGGGCCTTGAAGAACAAAGCCATGTTCAAACTCAACTGTTTGGTTAGAACTCATCATGGGGCCAAAGATGAGTCCTATGGTTGTTGCATACAACAAGAAGATGAGCAAGGGAGGCTTGGATTTCACCTTGCCAAGCATTTGCCCAAGGCTGCCACAAGAGCTTTTGTGGACAACCTTAGGGAAATTTCTCCCAAGATATTGCCCATCGGGGAATTGGTTAGGTTTATGCTTGCAACCTTTTTGAGGAAACTGAGACAAAGCTCATCCAACAAGCTAGGGACTACTCTCCCAAAACCTGTAATCAACTTCAAGACTGGGGTTGATCACTTCTGCATTCACACCGGCGGAAAGGCGGTGATAGATGGAATTGGCAAGAGTCTTGGACTCACTGAACATGACCTAGAGCCTGCAAGGATGACACTCCACAGATTTGGGAATACTTCTGCAAGTAGTCTCTGGTATGTCTTGGCTTACATGGAGGCCAAAAAGAGGCTAAAGAAGGGTGATACAGTGTTGATGATAAGCTTTGGTGCTGGCTTTAAATGCAACAGCTGTTTTCTGGAGGTGGTGAGAGATTTGGAGGATGGCAATGTGTGGAAAGATGAAATTGCTATGTACCCACCAAAGTCCTTGACGAACCCTTTCATGGAGAAATTTGGTTGGCTCCAACAAGAGGACCCAAGCACATTCAAGCTTGAGAATTTACTGTAA

Protein Analysis

466

Amino Acids

52.68

Weight (kDa)

8.72

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 25 - 312 7.6e-117 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 336 - 417 5.3e-15 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 339 - 417 3.3e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012947)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07720 AT1G07720 AT2G28630 AT2G28630
fragaria_vesca FvH4_3g34690
malus_domestica MD03G1102500.v1.1 MD11G1116500.v1.1
prunus_persica Prupe.6G086800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0062381
rosa_laevigata RLG00000035554
rosa_multiflora Rmu_sc0005798.1_g000006
rosa_roxburghii Rroxscaffold_1G00018280
rosa_rugosa Rorug05G0349200
rosa_samantha Rh5AG408700 Rh5BG423100 Rh5CG447000 Rh5DG437800
rosa_wichuraiana Rw5G038550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 476
AbsI CCTCGAGG 1 cut(s) 436
AccI GTMKAC 1 cut(s) 16
AciI CCGC 3 cut(s) 492, 1032, 1040
AclWI GGATC 3 cut(s) 269, 449, 618
AcsI RAATTY 4 cut(s) 181, 870, 1340, 1387
AcuI CTGAAG 1 cut(s) 567
AdeI CACNNNGTG 1 cut(s) 57
AfaI GTAC 3 cut(s) 200, 608, 1304
AfiI CCNNNNNNNGG 4 cut(s) 930, 1176, 1333, 1360
AgsI TTSAA 6 cut(s) 306, 547, 697, 715, 997, 1378
Alw21I GWGCWC 1 cut(s) 230
Alw26I GTCTC 3 cut(s) 75, 934, 1145
AlwI GGATC 3 cut(s) 269, 449, 618
Ama87I CYCGRG 1 cut(s) 436
AoxI GGCC 4 cut(s) 364, 690, 747, 1167
ApeKI GCWGC 1 cut(s) 836
ApoI RAATTY 4 cut(s) 181, 870, 1340, 1387
ArsI GACNNNNNNTTYG 2 cut(s) 166, 198
Asp700I GAANNNNTTC 1 cut(s) 1328
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 4 cut(s) 174, 690, 747, 1363
AsuHPI GGTGA 4 cut(s) 803, 1054, 1202, 1267
AvaI CYCGRG 1 cut(s) 436
AvaII GGWCC 2 cut(s) 174, 1363
AxyI CCTNAGG 1 cut(s) 863
BaeI ACNNNNGTAYC 2 cut(s) 598, 631
BanII GRGCYC 2 cut(s) 230, 599
BbsI GAAGAC 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 230
BbvCI CCTCAGC 1 cut(s) 488
BbvI GCAGC 1 cut(s) 823
BccI CCATC 5 cut(s) 310, 490, 899, 1043, 1265
BclI TGATCA 1 cut(s) 1009
BcoDI GTCTC 3 cut(s) 75, 934, 1145
BfaI CTAG 4 cut(s) 194, 510, 962, 1088
BfmI CTRYAG 1 cut(s) 274
BisI GCNGC 1 cut(s) 837
BlpI GCTNAGC 1 cut(s) 505
BlsI GCNGC 1 cut(s) 838
Bme18I GGWCC 2 cut(s) 174, 1363
BmeT110I CYCGRG 1 cut(s) 436
BmgT120I GGNCC 4 cut(s) 174, 690, 747, 1363
BmiI GGNNCC 3 cut(s) 748, 1352, 1365
BmrI ACTGGG 1 cut(s) 1011
BmuI ACTGGG 1 cut(s) 1011
BpiI GAAGAC 1 cut(s) 182
BpmI CTGGAG 1 cut(s) 1268
Bpu10I CCTNAGC 2 cut(s) 488, 593
Bpu1102I GCTNAGC 1 cut(s) 505
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 257, 831
Bsc4I CCNNNNNNNGG 4 cut(s) 930, 1176, 1333, 1360
Bse118I RCCGGY 1 cut(s) 1028
Bse1I ACTGG 3 cut(s) 202, 608, 1006
Bse21I CCTNAGG 1 cut(s) 863
Bse3DI GCAATG 1 cut(s) 1282
BseDI CCNNGG 2 cut(s) 257, 831
BseGI GGATG 5 cut(s) 501, 668, 950, 1107, 1276
BseLI CCNNNNNNNGG 4 cut(s) 930, 1176, 1333, 1360
BseMI GCAATG 1 cut(s) 1282
BseMII CTCAG 6 cut(s) 45, 502, 519, 534, 584, 929
BseNI ACTGG 3 cut(s) 202, 608, 1006
BseRI GAGGAG 2 cut(s) 200, 335
BseXI GCAGC 1 cut(s) 823
BshFI GGCC 4 cut(s) 366, 692, 749, 1169
BsiHKAI GWGCWC 1 cut(s) 230
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 2 cut(s) 231, 1029
BslFI GGGAC 2 cut(s) 574, 979
BslI CCNNNNNNNGG 4 cut(s) 930, 1176, 1333, 1360
BsmAI GTCTC 3 cut(s) 75, 934, 1145
BsmFI GGGAC 2 cut(s) 574, 979
BsmI GAATGC 3 cut(s) 116, 339, 1020
BsnI GGCC 4 cut(s) 366, 692, 749, 1169
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 1 cut(s) 436
Bsp1286I GDGCHC 2 cut(s) 230, 599
Bsp143I GATC 6 cut(s) 162, 261, 441, 516, 623, 1009
Bsp1720I GCTNAGC 1 cut(s) 505
BspACI CCGC 3 cut(s) 492, 1032, 1040
BspANI GGCC 4 cut(s) 366, 692, 749, 1169
BspCNI CTCAG 6 cut(s) 44, 501, 518, 533, 585, 930
BspLI GGNNCC 3 cut(s) 748, 1352, 1365
BspPI GGATC 3 cut(s) 269, 449, 618
BspTNI GGTCTC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 1282
BsrFI RCCGGY 1 cut(s) 1028
BsrI ACTGG 3 cut(s) 202, 608, 1006
BssAI RCCGGY 1 cut(s) 1028
BssECI CCNNGG 2 cut(s) 257, 831
BssMI GATC 6 cut(s) 162, 261, 441, 516, 623, 1009
BssNAI GTATAC 1 cut(s) 17
BssT1I CCWWGG 1 cut(s) 831
Bst1107I GTATAC 1 cut(s) 17
Bst4CI ACNGT 4 cut(s) 275, 725, 1198, 1395
BstC8I GCNNGC 4 cut(s) 242, 918, 1095, 1222
BstDEI CTNAG 8 cut(s) 11, 31, 488, 505, 520, 593, 863, 938
BstENI CCTNNNNNAGG 1 cut(s) 928
BstF5I GGATG 5 cut(s) 501, 668, 950, 1107, 1276
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 6 cut(s) 165, 264, 444, 519, 626, 1012
BstMAI GTCTC 3 cut(s) 75, 934, 1145
BstMBI GATC 6 cut(s) 162, 261, 441, 516, 623, 1009
BstMWI GCNNNNNNNGC 4 cut(s) 32, 799, 845, 1230
BstSFI CTRYAG 1 cut(s) 274
BstV1I GCAGC 1 cut(s) 823
BstV2I GAAGAC 1 cut(s) 182
BstX2I RGATCY 1 cut(s) 623
BstXI CCANNNNNNTGG 2 cut(s) 184, 1118
BstYI RGATCY 1 cut(s) 623
BstZ17I GTATAC 1 cut(s) 17
Bsu36I CCTNAGG 1 cut(s) 863
BsuRI GGCC 4 cut(s) 366, 692, 749, 1169
BtsCI GGATG 5 cut(s) 501, 668, 950, 1107, 1276
BtsIMutI CAGTG 2 cut(s) 1074, 1203
Cac8I GCNNGC 4 cut(s) 242, 918, 1095, 1222
CfoI GCGC 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 1028
Cfr13I GGNCC 4 cut(s) 174, 690, 747, 1363
Csp6I GTAC 3 cut(s) 199, 607, 1303
CviAII CATG 8 cut(s) 265, 285, 628, 709, 743, 1082, 1162, 1333
CviQI GTAC 3 cut(s) 199, 607, 1303
DdeI CTNAG 8 cut(s) 11, 31, 488, 505, 520, 593, 863, 938
DpnI GATC 6 cut(s) 164, 263, 443, 518, 625, 1011
DpnII GATC 6 cut(s) 162, 261, 441, 516, 623, 1009
DraI TTTAAA 1 cut(s) 1228
DraIII CACNNNGTG 1 cut(s) 57
DrdI GACNNNNNNGTC 1 cut(s) 476
DseDI GACNNNNNNGTC 1 cut(s) 476
EciI GGCGGA 1 cut(s) 1047
Ecl136II GAGCTC 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 831
Eco147I AGGCCT 1 cut(s) 366
Eco24I GRGCYC 2 cut(s) 230, 599
Eco31I GGTCTC 1 cut(s) 75
Eco47I GGWCC 2 cut(s) 174, 1363
Eco53kI GAGCTC 1 cut(s) 228
Eco57I CTGAAG 1 cut(s) 567
Eco81I CCTNAGG 1 cut(s) 863
Eco88I CYCGRG 1 cut(s) 436
EcoICRI GAGCTC 1 cut(s) 228
EcoNI CCTNNNNNAGG 1 cut(s) 928
EcoO109I RGGNCCY 2 cut(s) 690, 1363
EcoT14I CCWWGG 1 cut(s) 831
EcoT38I GRGCYC 2 cut(s) 230, 599
ErhI CCWWGG 1 cut(s) 831
FaeI CATG 8 cut(s) 268, 288, 631, 712, 746, 1085, 1165, 1336
FalI AAGNNNNNCTT 2 cut(s) 786, 818
FaqI GGGAC 2 cut(s) 574, 979
FatI CATG 8 cut(s) 264, 284, 627, 708, 742, 1081, 1161, 1332
FauI CCCGC 1 cut(s) 485
FbaI TGATCA 1 cut(s) 1009
FblI GTMKAC 1 cut(s) 16
Fnu4HI GCNGC 1 cut(s) 837
FokI GGATG 5 cut(s) 508, 675, 937, 1114, 1283
FriOI GRGCYC 2 cut(s) 230, 599
Fsp4HI GCNGC 1 cut(s) 837
FspBI CTAG 4 cut(s) 194, 510, 962, 1088
GlaI GCGC 1 cut(s) 255
GluI GCNGC 1 cut(s) 837
GsuI CTGGAG 1 cut(s) 1268
HaeIII GGCC 4 cut(s) 366, 692, 749, 1169
HapII CCGG 2 cut(s) 231, 1029
HhaI GCGC 1 cut(s) 256
Hin1II CATG 8 cut(s) 268, 288, 631, 712, 746, 1085, 1165, 1336
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HindIII AAGCTT 3 cut(s) 137, 1210, 1379
HinfI GANTC 7 cut(s) 281, 302, 587, 631, 758, 1063, 1071
HpaII CCGG 2 cut(s) 231, 1029
HphI GGTGA 4 cut(s) 803, 1054, 1202, 1267
Hpy166II GTNNAC 3 cut(s) 17, 609, 856
Hpy188I TCNGA 4 cut(s) 12, 345, 516, 586
Hpy188III TCNNGA 5 cut(s) 169, 194, 535, 997, 1247
Hpy8I GTNNAC 3 cut(s) 17, 609, 856
HpyAV CCTTC 4 cut(s) 340, 384, 435, 1180
HpyCH4III ACNGT 4 cut(s) 275, 725, 1198, 1395
HpyCH4V TGCA 8 cut(s) 569, 638, 774, 920, 1020, 1097, 1133, 1233
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 799, 845, 1230
HpyF3I CTNAG 8 cut(s) 11, 31, 488, 505, 520, 593, 863, 938
Hsp92II CATG 8 cut(s) 268, 288, 631, 712, 746, 1085, 1165, 1336
HspAI GCGC 1 cut(s) 254
Ksp22I TGATCA 1 cut(s) 1009
Kzo9I GATC 6 cut(s) 162, 261, 441, 516, 623, 1009
LmnI GCTCC 3 cut(s) 233, 537, 1356
Lsp1109I GCAGC 1 cut(s) 823
MaeI CTAG 4 cut(s) 194, 510, 962, 1088
MaeIII GTNAC 2 cut(s) 92, 577
MalI GATC 6 cut(s) 164, 263, 443, 518, 625, 1011
MboI GATC 6 cut(s) 162, 261, 441, 516, 623, 1009
MboII GAAGA 5 cut(s) 182, 311, 319, 709, 797
MflI RGATCY 1 cut(s) 623
MhlI GDGCHC 2 cut(s) 230, 599
MluCI AATT 8 cut(s) 181, 640, 870, 899, 1053, 1293, 1340, 1387
MlyI GAGTC 4 cut(s) 596, 767, 1065, 1072
MmeI TCCRAC 3 cut(s) 333, 978, 1378
MroXI GAANNNNTTC 1 cut(s) 1328
MseI TTAA 1 cut(s) 1227
MslI CAYNNNNRTG 1 cut(s) 314
MspA1I CMGCKG 2 cut(s) 492, 1239
MspI CCGG 2 cut(s) 231, 1029
Mva1269I GAATGC 3 cut(s) 116, 339, 1020
MwoI GCNNNNNNNGC 4 cut(s) 32, 799, 845, 1230
NdeII GATC 6 cut(s) 162, 261, 441, 516, 623, 1009
NlaIII CATG 8 cut(s) 268, 288, 631, 712, 746, 1085, 1165, 1336
NlaIV GGNNCC 3 cut(s) 748, 1352, 1365
NmeAIII GCCGAG 1 cut(s) 282
PaeR7I CTCGAG 1 cut(s) 436
PceI AGGCCT 1 cut(s) 366
PctI GAATGC 3 cut(s) 116, 339, 1020
PdmI GAANNNNTTC 1 cut(s) 1328
PfeI GAWTC 3 cut(s) 281, 302, 631
PkrI GCNGC 1 cut(s) 838
PleI GAGTC 4 cut(s) 595, 766, 1065, 1071
PpsI GAGTC 4 cut(s) 595, 766, 1065, 1071
PpuMI RGGWCCY 1 cut(s) 1363
Psp124BI GAGCTC 1 cut(s) 230
Psp5II RGGWCCY 1 cut(s) 1363
PspN4I GGNNCC 3 cut(s) 748, 1352, 1365
PspPI GGNCC 4 cut(s) 174, 690, 747, 1363
PspPPI RGGWCCY 1 cut(s) 1363
PspXI VCTCGAGB 1 cut(s) 436
PsuI RGATCY 1 cut(s) 623
PvuII CAGCTG 1 cut(s) 1239
RsaI GTAC 3 cut(s) 200, 608, 1304
RsaNI GTAC 3 cut(s) 199, 607, 1303
RseI CAYNNNNRTG 1 cut(s) 314
SacI GAGCTC 1 cut(s) 230
SaqAI TTAA 1 cut(s) 1227
SatI GCNGC 1 cut(s) 837
Sau3AI GATC 6 cut(s) 162, 261, 441, 516, 623, 1009
Sau96I GGNCC 4 cut(s) 174, 690, 747, 1363
SchI GAGTC 4 cut(s) 596, 767, 1065, 1072
SduI GDGCHC 2 cut(s) 230, 599
SfcI CTRYAG 1 cut(s) 274
Sfr274I CTCGAG 1 cut(s) 436
SgrAI CRCCGGYG 1 cut(s) 1028
SinI GGWCC 2 cut(s) 174, 1363
SlaI CTCGAG 1 cut(s) 436
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 2 cut(s) 436, 1382
SmoI CTYRAG 2 cut(s) 436, 1382
Sse9I AATT 8 cut(s) 181, 640, 870, 899, 1053, 1293, 1340, 1387
SseBI AGGCCT 1 cut(s) 366
SsiI CCGC 3 cut(s) 492, 1032, 1040
SspMI CTAG 4 cut(s) 194, 510, 962, 1088
SstI GAGCTC 1 cut(s) 230
StuI AGGCCT 1 cut(s) 366
StyI CCWWGG 1 cut(s) 831
TaaI ACNGT 4 cut(s) 275, 725, 1198, 1395
TaqI TCGA 2 cut(s) 270, 437
TasI AATT 8 cut(s) 181, 640, 870, 899, 1053, 1293, 1340, 1387
TfiI GAWTC 3 cut(s) 281, 302, 631
Tru1I TTAA 1 cut(s) 1227
Tru9I TTAA 1 cut(s) 1227
TscAI CASTG 2 cut(s) 1081, 1203
TseI GCWGC 1 cut(s) 836
TspDTI ATGAA 4 cut(s) 34, 87, 1305, 1321
TspRI CASTG 2 cut(s) 1081, 1203
VpaK11BI GGWCC 2 cut(s) 174, 1363
XagI CCTNNNNNAGG 1 cut(s) 928
XapI RAATTY 4 cut(s) 181, 870, 1340, 1387
XbaI TCTAGA 1 cut(s) 193
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XhoI CTCGAG 1 cut(s) 436
XmiI GTMKAC 1 cut(s) 16
XmnI GAANNNNTTC 1 cut(s) 1328
XspI CTAG 4 cut(s) 194, 510, 962, 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.